Stx5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stx5
Entrez Gene ID	65134
Full Name	syntaxin 5
Synonyms	Stx5a
General protein information	Preferred Names syntaxin 5 Names syntaxin-5 syntaxin 5a
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	component of the target-membrane associated SNARE (t-SNARE) complex; binds synaptobrevin/VAMP; plays a role in vesicular transport from the endoplasmic reticulum to the golgi [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031704.2	NP_113892.2	syntaxin-5
XM_006230997.2	XP_006231059.1	syntaxin-5 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	STX5	XP_005631656.1
Bos taurus (cattle)	STX5	NP_001068912.1
Drosophila melanogaster (fruit fly)	Syx5	NP_523582.4
Caenorhabditis elegans (roundworm)	syx-5	NP_505968.1
Xenopus tropicalis (tropical clawed frog)	stx5	NP_001007991.1
	STX5	XP_002799556.1
Mus musculus (house mouse)	Stx5a	NP_062803.4
Danio rerio (zebrafish)	stx5al	NP_955924.1
Schizosaccharomyces pombe (fission yeast)	sed5	NP_595576.1
Arabidopsis thaliana (thale cress)	SYP32	NP_001189961.1
Oryza sativa (rice)	Os01g0179200	NP_001042197.1
Homo sapiens (human)	STX5	NP_003155.2
Rattus norvegicus (Norway rat)	Stx5	NP_113892.2
Saccharomyces cerevisiae (baker's yeast)	SED5	NP_013126.1
Magnaporthe oryzae (rice blast fungus)	MGG_02920	XP_003720799.1
Neurospora crassa	NCU01907	XP_965538.2
Pan troglodytes (chimpanzee)	STX5	XP_508504.2
Kluyveromyces lactis	KLLA0F17798g	XP_455878.1
Danio rerio (zebrafish)	stx5a	NP_001002333.1
Eremothecium gossypii	AGOS_AFR210C	NP_985757.2

Gene Ontology
Bibliography

Related articles in PubMed

Syntaxin 5 Overexpression and ?-Amyloid 1-42 Accumulation in Endoplasmic Reticulum of Hippocampal Cells in Rat Brain Induced by Ozone Exposure.
Hern?ndez-Zimbr?n LF, Rivas-Arancibia S
BioMed research international20162125643(2016)

ER stress response in NG108-15 cells involves upregulation of syntaxin 5 expression and reduced amyloid ? peptide secretion.
Suga K, Saito A, Akagawa K
Experimental cell research332(1)11-23(2015 Mar)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.
Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC
Journal of cell science124(Pt 3)469-82(2011 Feb)

Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.
Laufman O, Kedan A, Hong W, Lev S
The EMBO journal28(14)2006-17(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

O3 exposure (0.25 ppm) induced a significant increase of Syntaxin 5 and accumulation of betaA42 peptide in the reticulum in cells of the dentate gyrus
Title: Syntaxin 5 Overexpression and ?-Amyloid 1-42 Accumulation in Endoplasmic Reticulum of Hippocampal Cells in Rat Brain Induced by Ozone Exposure.

Syx5 plays important roles in beta-amyloid precursor protein processing and in the endoplasmic reticulum stress response.
Title: ER stress response in NG108-15 cells involves upregulation of syntaxin 5 expression and reduced amyloid ? peptide secretion.

depletion of GM130 by RNA interference slows the rate of ER to Golgi trafficking in vivo; interactions of GM130 with syntaxin 5 and Rab1 are regulated by mitotic phosphorylation
Title: Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner.

elucidated the structure in solution of the isolated N-terminal domain of rat Sly1 and analyzed its complex with an N-terminal peptide of rat syntaxin 5 by NMR spectroscopy
Title: Three-dimensional structure of the rSly1 N-terminal domain reveals a conformational change induced by binding to syntaxin 5.

The following Stx5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stx5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08524	NM_031704.2 Latest version!	Rattus norvegicus syntaxin 5 (Stx5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa08524	XM_006230997.2 Latest version!	Rattus norvegicus syntaxin 5 (Stx5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stx5 ( NM_031704.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113892.2 Rattus norvegicus syntaxin 5 (Stx5), mRNA.

Price & Availability↑

CloneID	ORa08524
Clone ID Related Accession (Same CDS sequence)	NM_031704.2 , XM_006230997.2
Accession Version	NM_031704.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	syntaxin-5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC127489.1. On Apr 9, 2013 this sequence version replaced NM_031704.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC127489.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATCCCGC GGAAACGCTA CGGATCTAAG AACACGGATC AGGGTGTCTA CCTGGGTCTC 
TCAAAGACAC AGGTTCTGTC CCCTGCAACT GCTATCAATA GCAGCAGTGA CATCGCTCCT 
TTGCCCACCC CGGTGGCCCT GGTCCCCTCC CCTCCCGACA CTATGTCCTG CCGGGATCGG 
ACCCAGGAGT TCCTGTCTGC CTGCAAGTCG CTGCAGAGCC GTCAGAATGG AATCCAAACA 
AATAAACCAG CTCTGCACGC TACCCGGCAG TGCAGCGAGT TTACCCTCAT GGCCAGGCGC 
ATTGGGAAAG ATCTCAGCAA TACATTTGCC AAGCTGGAGA AGCTAACAAT CCTGGCAAAG 
CGCAAGTCCC TCTTTGATGA TAAAGCAGTA GAAATTGAGG AACTAACATA CATCATCAAA 
CAGGACATCA ATAGCCTCAA CAAACAAATT GCTCAGCTTC AAGATTTTGT GAGGGCCAAG 
GGCAGCCAGA GTGGCCGGCA TCTGCAGACC CATTCCAACA CCATTGTAGT TTCATTGCAG 
TCAAAACTGG CTTCCATGTC CAATGACTTC AAGTCTGTTT TGGAAGTGAG GACTGAAAAT 
CTGAAACAGC AGAGGAACCG TCGGGAACAG TTCTCCAGGG CGCCAGTGTC GGCACTGCCT 
CTGGCCCCCA ACAACCTTGG AGGTGGTCCC ATAGTTCTGG GAGGAGAGTC CCGAGCCTCC 
AGGGACGTGG CCATCGACAT GATGGACCCT AGAACAAGCC AGCAGCTTCA GCTCATTGAT 
GAGCAGGATT CCTACATCCA GAGTCGGGCA GACACCATGC AGAACATTGA GTCTACAATT 
GTTGAGCTGG GCTCCATTTT TCAACAATTG GCACACATGG TTAAAGAACA GGAGGAAACA 
ATTCAGAGGA TTGACGAGAA TGTGCTTGGA GCCCAGCTGG ATGTTGAGGC CGCCCATTCA 
GAGATCCTCA AGTACTTCCA GTCAGTCACC TCCAATCGGT GGCTCATGGT CAAAATCTTC 
CTCATCCTCA TTGTCTTCTT CATCATCTTT GTGGTCTTCC TTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113892.2
CDS	138..1205
Translation	MIPRKRYGSKNTDQGVYLGLSKTQVLSPATAINSSSDIAPLPTPVALVPSPPDTMSCRDRTQEFLSACKSLQSRQNGIQTNKPALHATRQCSEFTLMARRIGKDLSNTFAKLEKLTILAKRKSLFDDKAVEIEELTYIIKQDINSLNKQIAQLQDFVRAKGSQSGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRNRREQFSRAPVSALPLAPNNLGGGPIVLGGESRASRDVAIDMMDPRTSQQLQLIDEQDSYIQSRADTMQNIESTIVELGSIFQQLAHMVKEQEETIQRIDENVLGAQLDVEAAHSEILKYFQSVTSNRWLMVKIFLILIVFFIIFVVFLA

Target ORF information:

RefSeq Version	NM_031704.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus syntaxin 5 (Stx5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031704.2

ORF Insert Sequence:

ATGATCCCGC GGAAACGCTA CGGATCTAAG AACACGGATC AGGGTGTCTA CCTGGGTCTC 
TCAAAGACAC AGGTTCTGTC CCCTGCAACT GCTATCAATA GCAGCAGTGA CATCGCTCCT 
TTGCCCACCC CGGTGGCCCT GGTCCCCTCC CCTCCCGACA CTATGTCCTG CCGGGATCGG 
ACCCAGGAGT TCCTGTCTGC CTGCAAGTCG CTGCAGAGCC GTCAGAATGG AATCCAAACA 
AATAAACCAG CTCTGCACGC TACCCGGCAG TGCAGCGAGT TTACCCTCAT GGCCAGGCGC 
ATTGGGAAAG ATCTCAGCAA TACATTTGCC AAGCTGGAGA AGCTAACAAT CCTGGCAAAG 
CGCAAGTCCC TCTTTGATGA TAAAGCAGTA GAAATTGAGG AACTAACATA CATCATCAAA 
CAGGACATCA ATAGCCTCAA CAAACAAATT GCTCAGCTTC AAGATTTTGT GAGGGCCAAG 
GGCAGCCAGA GTGGCCGGCA TCTGCAGACC CATTCCAACA CCATTGTAGT TTCATTGCAG 
TCAAAACTGG CTTCCATGTC CAATGACTTC AAGTCTGTTT TGGAAGTGAG GACTGAAAAT 
CTGAAACAGC AGAGGAACCG TCGGGAACAG TTCTCCAGGG CGCCAGTGTC GGCACTGCCT 
CTGGCCCCCA ACAACCTTGG AGGTGGTCCC ATAGTTCTGG GAGGAGAGTC CCGAGCCTCC 
AGGGACGTGG CCATCGACAT GATGGACCCT AGAACAAGCC AGCAGCTTCA GCTCATTGAT 
GAGCAGGATT CCTACATCCA GAGTCGGGCA GACACCATGC AGAACATTGA GTCTACAATT 
GTTGAGCTGG GCTCCATTTT TCAACAATTG GCACACATGG TTAAAGAACA GGAGGAAACA 
ATTCAGAGGA TTGACGAGAA TGTGCTTGGA GCCCAGCTGG ATGTTGAGGC CGCCCATTCA 
GAGATCCTCA AGTACTTCCA GTCAGTCACC TCCAATCGGT GGCTCATGGT CAAAATCTTC 
CTCATCCTCA TTGTCTTCTT CATCATCTTT GTGGTCTTCC TTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stx5 ( XM_006230997.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231059.1 Rattus norvegicus syntaxin 5 (Stx5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa08524
Clone ID Related Accession (Same CDS sequence)	NM_031704.2 , XM_006230997.2
Accession Version	XM_006230997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	syntaxin-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006230997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCGC GGAAACGCTA CGGATCTAAG AACACGGATC AGGGTGTCTA CCTGGGTCTC 
TCAAAGACAC AGGTTCTGTC CCCTGCAACT GCTATCAATA GCAGCAGTGA CATCGCTCCT 
TTGCCCACCC CGGTGGCCCT GGTCCCCTCC CCTCCCGACA CTATGTCCTG CCGGGATCGG 
ACCCAGGAGT TCCTGTCTGC CTGCAAGTCG CTGCAGAGCC GTCAGAATGG AATCCAAACA 
AATAAACCAG CTCTGCACGC TACCCGGCAG TGCAGCGAGT TTACCCTCAT GGCCAGGCGC 
ATTGGGAAAG ATCTCAGCAA TACATTTGCC AAGCTGGAGA AGCTAACAAT CCTGGCAAAG 
CGCAAGTCCC TCTTTGATGA TAAAGCAGTA GAAATTGAGG AACTAACATA CATCATCAAA 
CAGGACATCA ATAGCCTCAA CAAACAAATT GCTCAGCTTC AAGATTTTGT GAGGGCCAAG 
GGCAGCCAGA GTGGCCGGCA TCTGCAGACC CATTCCAACA CCATTGTAGT TTCATTGCAG 
TCAAAACTGG CTTCCATGTC CAATGACTTC AAGTCTGTTT TGGAAGTGAG GACTGAAAAT 
CTGAAACAGC AGAGGAACCG TCGGGAACAG TTCTCCAGGG CGCCAGTGTC GGCACTGCCT 
CTGGCCCCCA ACAACCTTGG AGGTGGTCCC ATAGTTCTGG GAGGAGAGTC CCGAGCCTCC 
AGGGACGTGG CCATCGACAT GATGGACCCT AGAACAAGCC AGCAGCTTCA GCTCATTGAT 
GAGCAGGATT CCTACATCCA GAGTCGGGCA GACACCATGC AGAACATTGA GTCTACAATT 
GTTGAGCTGG GCTCCATTTT TCAACAATTG GCACACATGG TTAAAGAACA GGAGGAAACA 
ATTCAGAGGA TTGACGAGAA TGTGCTTGGA GCCCAGCTGG ATGTTGAGGC CGCCCATTCA 
GAGATCCTCA AGTACTTCCA GTCAGTCACC TCCAATCGGT GGCTCATGGT CAAAATCTTC 
CTCATCCTCA TTGTCTTCTT CATCATCTTT GTGGTCTTCC TTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231059.1
CDS	681..1748
Translation	MIPRKRYGSKNTDQGVYLGLSKTQVLSPATAINSSSDIAPLPTPVALVPSPPDTMSCRDRTQEFLSACKSLQSRQNGIQTNKPALHATRQCSEFTLMARRIGKDLSNTFAKLEKLTILAKRKSLFDDKAVEIEELTYIIKQDINSLNKQIAQLQDFVRAKGSQSGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRNRREQFSRAPVSALPLAPNNLGGGPIVLGGESRASRDVAIDMMDPRTSQQLQLIDEQDSYIQSRADTMQNIESTIVELGSIFQQLAHMVKEQEETIQRIDENVLGAQLDVEAAHSEILKYFQSVTSNRWLMVKIFLILIVFFIIFVVFLA

Target ORF information:

RefSeq Version	XM_006230997.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus syntaxin 5 (Stx5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230997.2

ORF Insert Sequence:

ATGATCCCGC GGAAACGCTA CGGATCTAAG AACACGGATC AGGGTGTCTA CCTGGGTCTC 
TCAAAGACAC AGGTTCTGTC CCCTGCAACT GCTATCAATA GCAGCAGTGA CATCGCTCCT 
TTGCCCACCC CGGTGGCCCT GGTCCCCTCC CCTCCCGACA CTATGTCCTG CCGGGATCGG 
ACCCAGGAGT TCCTGTCTGC CTGCAAGTCG CTGCAGAGCC GTCAGAATGG AATCCAAACA 
AATAAACCAG CTCTGCACGC TACCCGGCAG TGCAGCGAGT TTACCCTCAT GGCCAGGCGC 
ATTGGGAAAG ATCTCAGCAA TACATTTGCC AAGCTGGAGA AGCTAACAAT CCTGGCAAAG 
CGCAAGTCCC TCTTTGATGA TAAAGCAGTA GAAATTGAGG AACTAACATA CATCATCAAA 
CAGGACATCA ATAGCCTCAA CAAACAAATT GCTCAGCTTC AAGATTTTGT GAGGGCCAAG 
GGCAGCCAGA GTGGCCGGCA TCTGCAGACC CATTCCAACA CCATTGTAGT TTCATTGCAG 
TCAAAACTGG CTTCCATGTC CAATGACTTC AAGTCTGTTT TGGAAGTGAG GACTGAAAAT 
CTGAAACAGC AGAGGAACCG TCGGGAACAG TTCTCCAGGG CGCCAGTGTC GGCACTGCCT 
CTGGCCCCCA ACAACCTTGG AGGTGGTCCC ATAGTTCTGG GAGGAGAGTC CCGAGCCTCC 
AGGGACGTGG CCATCGACAT GATGGACCCT AGAACAAGCC AGCAGCTTCA GCTCATTGAT 
GAGCAGGATT CCTACATCCA GAGTCGGGCA GACACCATGC AGAACATTGA GTCTACAATT 
GTTGAGCTGG GCTCCATTTT TCAACAATTG GCACACATGG TTAAAGAACA GGAGGAAACA 
ATTCAGAGGA TTGACGAGAA TGTGCTTGGA GCCCAGCTGG ATGTTGAGGC CGCCCATTCA 
GAGATCCTCA AGTACTTCCA GTCAGTCACC TCCAATCGGT GGCTCATGGT CAAAATCTTC 
CTCATCCTCA TTGTCTTCTT CATCATCTTT GTGGTCTTCC TTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Syntaxin 5 Overexpression and ?-Amyloid 1-42 Accumulation in Endoplasmic Reticulum of Hippocampal Cells in Rat Brain Induced by Ozone Exposure.
BioMed research international20162125643(2016)
Hern?ndez-Zimbr?n LF,Rivas-Arancibia S

ER stress response in NG108-15 cells involves upregulation of syntaxin 5 expression and reduced amyloid ? peptide secretion.
Experimental cell research332(1)11-23(2015 Mar)
Suga K,Saito A,Akagawa K

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.
Journal of cell science124(Pt 3)469-82(2011 Feb)
Renna M,Schaffner C,Winslow AR,Menzies FM,Peden AA,Floto RA,Rubinsztein DC

Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.
The EMBO journal28(14)2006-17(2009 Jul)
Laufman O,Kedan A,Hong W,Lev S