Slit1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slit1
Entrez Gene ID	65047
Full Name	slit guidance ligand 1
Synonyms	MEGF4
General protein information	Preferred Names slit guidance ligand 1 Names slit homolog 1 protein multiple EGF-like domains protein 4 multiple epidermal growth factor-like domains 4 multiple epidermal growth factor-like domains protein 4 slit-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54
Summary	homolog of Drosophila slit; may play a role in protein-protein interactions during midline formation in the central nervous system; specifically expressed in fetal and adult forebrain neurons [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589694.1	XP_017445183.1	slit homolog 1 protein isoform X1
NM_022953.2	NP_075242.1	slit homolog 1 protein precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLIT1	XP_003312806.1
Homo sapiens (human)	SLIT1	NP_003052.2
Bos taurus (cattle)	SLIT1	XP_002698459.1
Xenopus tropicalis (tropical clawed frog)	slit1	NP_001090702.1
	SLIT1	XP_001094231.2
Canis lupus familiaris (dog)	SLIT1	XP_005637640.1
Mus musculus (house mouse)	Slit1	NP_056563.2
Rattus norvegicus (Norway rat)	Slit1	NP_075242.1
Gallus gallus (chicken)	SLIT1	NP_001264265.1
Danio rerio (zebrafish)	slit1a	NP_001030140.1

Gene Ontology
Bibliography

Related articles in PubMed

Slit1 promotes regenerative neurite outgrowth of adult dorsal root ganglion neurons in vitro via binding to the Robo receptor.
Zhang HY, Zheng LF, Yi XN, Chen ZB, He ZP, Zhao D, Zhang XF, Ma ZJ
Journal of chemical neuroanatomy39(4)256-61(2010 Jul)

mRNA expression changes of slit proteins following peripheral nerve injury in the rat model.
Fujiwara T, Kubo T, Koyama Y, Tomita K, Yano K, Tohyama M, Hosokawa K
Journal of chemical neuroanatomy36(3-4)170-6(2008 Dec)

Axonal growth regulation of fetal and embryonic stem cell-derived dopaminergic neurons by Netrin-1 and Slits.
Lin L, Isacson O
Stem cells (Dayton, Ohio)24(11)2504-13(2006 Nov)

Slit proteins regulate distinct aspects of retinal ganglion cell axon guidance within dorsal and ventral retina.
Thompson H, Camand O, Barker D, Erskine L
The Journal of neuroscience : the official journal of the Society for Neuroscience26(31)8082-91(2006 Aug)

Slits contribute to the guidance of retinal ganglion cell axons in the mammalian optic tract.
Thompson H, Barker D, Camand O, Erskine L
Developmental biology296(2)476-84(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicate that Slit1/Robo2 signaling may have a role in regulating peripheral nerve regeneration.
Title: Slit1 promotes regenerative neurite outgrowth of adult dorsal root ganglion neurons in vitro via binding to the Robo receptor.

The difference in expression pattern of Slit family mRNA in the neurons during peripheral nerve regeneration suggests that it plays a different role in axonal regeneration after axotomy of peripheral nerves
Title: mRNA expression changes of slit proteins following peripheral nerve injury in the rat model.

The novel splicing product, Slit1alpha, arises through alternative splicing at the C-terminus of Slit1, causing defects in the cysteine knot domain.
Title: Expression and function of Slit1alpha, a novel alternative splicing product for slit1.

Ig domains 1 and 2 of Robo1 are important for Robo-Slit interaction and provide functional data using the Slit-mediated olfactory bulb repulsion assay.
Title: Extracellular Ig domains 1 and 2 of Robo are important for ligand (Slit) binding.

The following Slit1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slit1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa56831	XM_017589694.1 Latest version!	Rattus norvegicus slit guidance ligand 1 (Slit1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa06527	NM_022953.2 Latest version!	Rattus norvegicus slit guidance ligand 1 (Slit1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1126.30 $1609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slit1 ( XM_017589694.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445183.1 Rattus norvegicus slit guidance ligand 1 (Slit1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56831
Clone ID Related Accession (Same CDS sequence)	XM_017589694.1
Accession Version	XM_017589694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	slit homolog 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAGC TTCGGACCTT CCGGCTGCAC TCCAACCACC TGTTCTGTGA CTGTCACCTG 
GCCTGGCTCT CGCAGTGGCT GAGACAGAGG CCCACCATCG GGCTCTTCAC CCAGTGCTCT 
GGGCCCGCCA GCCTTCGGGG CCTCAATGTG GCAGAGGTGC AAAAGAGTGA GTTCAGCTGC 
TCAGGCCAGG GGGAGGCTGC ACAAGTACCT GCCTGCACCC TCTCATCCGG TTCCTGCCCA 
GCCATGTGCA GCTGTAGCAA TGGCATCGTG GACTGCCGTG GCAAAGGCCT CACTGCCATC 
CCTGCTAACC TTCCGGAGAC GATGACCGAG ATCCGCCTGG AGCTGAACGG TATCAAATCC 
ATCCCACCAG GAGCCTTCTC TCCCTACAGA AAGCTGCGCA GGATAGACCT GAGTAACAAC 
CAGATTGCTG AGATTGCACC CGATGCCTTC CAAGGCCTCC GCTCCTTGAA TTCCCTGGTC 
CTCTATGGCA ACAAGATCAC AGACTTGCCC CGAGGTGTAT TTGGAGGCCT GTACACCCTC 
CAGCTGCTGC TTCTGAATGC CAACAAGATC AACTGCATCC GGCCTGATGC CTTCCAGGAC 
CTGCAGAACC TCTCTCTGCT CTCCCTATAT GACAACAAGA TCCAGAGTCT CGCCAAGGGC 
ACCTTCACCT CCCTGCGGGC CATCCAGACA CTGCATCTGG CCCAGAACCC TTTCATCTGT 
GACTGTAACC TCAAGTGGCT AGCAGACTTC TTACGTACCA ACCCCATTGA GACAACTGGG 
GCCCGCTGTG CCAGCCCCCG GCGCCTTGCC AACAAGCGTA TCGGGCAGAT CAAAAGCAAG 
AAGTTCCGGT GCTCAGCCAA AGAGCAGTAC TTCATTCCAG GGACGGAGGA TTACCACCTG 
AACAGCGAGT GTACGAGTGA CGTGGCCTGT CCCCACAAGT GCCGCTGTGA GGCCAGTGTG 
GTGGAGTGCT CTGGCCTGAA GCTCTCTAAG ATCCCTGAGC GAATCCCCCA GTCCACGACT 
GAGCTACGAC TGAACAACAA TGAGATTTCA ATCCTAGAGG CCACTGGGTT GTTTAAGAAG 
CTCTCACATC TGAAGAAAAT AAACCTGAGC AACAACAAGG TGTCAGAGAT TGAAGACGGG 
ACGTTTGAAG GCGCCACCTC CGTCAGCGAG CTGCACCTGA CCGCCAACCA GCTGGAGTCT 
GTCAGAAGCG GCATGTTCCG GGGTCTAGAT GGCTTGCGGA CCCTGATGCT GAGGAACAAC 
CGTATCAGCT GCATCCACAA TGACAGCTTC ACTGGGCTGC GTAACGTGCG GCTCCTGTCT 
CTTTACGACA ACCACATCAC CACCATCTCC CCTGGAGCCT TCGACACCCT CCAGGCCCTG 
TCCACACTAA ACCTCCTGGC CAACCCATTC AACTGCAACT GCCAGTTGGC CTGGCTGGGG 
GACTGGCTTC GGAAGAGGAA AATTGTGACA GGGAACCCTC GCTGTCAGAA CCCAGACTTC 
CTGCGGCAGA TTCCTCTGCA AGATGTGGCC TTCCCTGACT TCAGGTGTGA GGAAGGCCAG 
GAGGAGGTGG GCTGCCTGCC CCGCCCACAG TGCCCCCAAG AGTGCGCCTG CCTGGACACC 
GTGGTCCGGT GTAGCAACAA GCACCTCCAG GCTCTGCCCA AGGGCATCCC CAAGAATGTC 
ACAGAACTCT ACTTAGATGG GAACCAGTTC ACACTGGTTC CGGGACAGCT GTCTACCTTC 
AAGTATCTGC AGCTTGTCGA CCTGAGCAAC AACAAGATCA GCTCCTTAAG CAACTCTTCC 
TTCACCAACA TGAGTCAGCT GACCACACTG ATCCTCAGCT ACAACGCCCT CCAGTGCATC 
CCCCCTCTGG CCTTCCAAGG ACTTCGTTCA CTGCGCTTGC TGTCTTTGCA TGGCAATGAT 
GTCTCCACCC TCCAAGAGGG AATCTTTGCA GACGTGACGT CTCTGTCTCA CCTGGCCATT 
GGTGCCAACC CCCTGTACGA CTGCCACCTC CGATGGCTCT CCAGCTGGGT GAAGACTGGC 
TACAAGGAAC CTGGCATTGC CCGCTGTGCT GGACCCCCAG AGATGGAGGG CAAACTGTTG 
CTCACCACAC CTGCCAAGAA GTTTGAATGC CAAGGTCCCC CCAGCCTGGC GGTTCAGGCC 
AAGTGTGATC CCTGCTTGTC CAGCCCATGC CAGAATCAGG GCACTTGTCA CAATGACCCT 
CTTGAGGTAT ACAGGTGTAC CTGCCCCAGT GGCTACAAGG GCCGAAACTG TGAGGTGTCT 
CTGGACAGCT GTTCCAGCAA CCCATGTGGA AATGGGGGAA CCTGCCATGC CCAGGAGGGC 
GAGGATGCTG GCTTCACGTG CTCCTGTCCT TCCGGCTTTG AAGGGCTGAC GTGTGGAATG 
AACACAGATG ATTGTGTGAA GCACGACTGT GTCAATGGGG GTGTCTGTGT GGATGGCATA 
GGGAATTACA CATGTCAGTG CCCCCTGCAG TACACAGGAA GGGCCTGTGA ACAGCTAGTG 
GACTTCTGCT CTCCGGATCT GAACCCATGT CAGCATGAGG CCCAATGTGT TGGCACCCCA 
GAGGGGCCCA GGTGTGAGTG TGTGCCAGGC TACACGGGTG ACAATTGCAG CAAAAACCAG 
GACGACTGCA AGGACCACCA GTGTCAGAAC GGGGCTCAGT GTGTGGATGA GATCAACAGC 
TACGCCTGTC TCTGTGCTGA GGGCTACAGC GGACAGCTCT GTGAGATCCC ACCTGCCCCC 
AGGAACTCCT GTGAAGGGAC TGAGTGCCAG AATGGAGCCA ACTGTGTAGA CCAGGGCAGC 
CGGCCCGTAT GCCAGTGCCT ACCAGGCTTT GGAGGTCCCG AATGTGAGAA GTTGCTCAGT 
GTCAACTTTG TGGACCGGGA CACTTACCTG CAGTTCACTG ACCTGCAGAA CTGGCCTCGG 
GCCAACATCA CTCTTCAGGT CTCCACAGCA GAGGACAATG GGATCCTCCT CTACAATGGG 
GATAATGACC ACATTGCAGT TGAGCTGTAC CAGGGCCATG TCCGTGTTAG CTACGACCCA 
GGCAGCTACC CCAGCTCTGC TATCTACAGT GCTGAAACAA TCAACGATGG GCAGTTCCAC 
ACAGTTGAGC TGGTGACCTT TGACCAGATG GTGAACCTCT CCATTGATGG TGGCAGCCCC 
ATGACCATGG ACAACTTTGG AAAGCACTAC ACACTCAACA GTGAGGCCCC CCTCTATGTG 
GGAGGGATGC CCGTGGATGT GAACTCAGCT GCCTTCCGCC TGTGGCAGAT CCTCAATGGC 
ACCAGCTTCC ACGGTTGCAT CCGGAATCTA TACATCAACA ACGAACTGCA GGACTTCACC 
AAGACACAGA TGAAGCCGGG CGTGGTGCCC GGCTGCGAGC CCTGCCGAAA ACTCTACTGT 
CTACATGGCA TTTGCCAGCC CAACGCCACC CCAGGGCCCG TGTGCCACTG CGAGGCTGGC 
TGGGGGGGCC TGCACTGTGA CCAGCCAGTG GACGGCCCCT GCCATGGCCA CAAGTGTGTC 
CATGGGAAAT GCGTGCCCCT CGATGCACTT GCCTACAGCT GCCAATGCCA GGATGGATAC 
TCGGGGGCTC TGTGCAACCA GGTCGGGGCT GTGGCAGAGC CCTGTGGGGG CTTACAGTGC 
CTGCATGGTC ACTGCCAGGC CTCGGCCACC AGAGGGGCAC ACTGTGTGTG CAGCCCAGGT 
TTTTCAGGCG AGCTGTGTGA GCAAGAGTCC GAGTGCCGGG GGGACCCTGT CCGGGACTTT 
CACCGGGTCC AGAGGGGCTA TGCCATCTGC CAGACCACGC GCCCACTGTC ATGGGTGGAA 
TGCCGGGGCG CGTGCCCGGG CCAGGGCTGC TGCCAGGGCC TGCGGCTGAA GCGGAGGAAG 
CTCACCTTCG AGTGCAGCGA TGGGACCTCG TTTGCTGAGG AGGTGGAGAA GCCCACCAAG 
TGCGGCTGTG CCCCGTGTGC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445183.1
CDS	564..4547
Translation	MPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAAQVPACTLSSGSCPAMCSCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSGLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGATSVSELHLTANQLESVRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCQLAWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYDCHLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFECQGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRNCEVSLDSCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGLTCGMNTDDCVKHDCVNGGVCVDGIGNYTCQCPLQYTGRACEQLVDFCSPDLNPCQHEAQCVGTPEGPRCECVPGYTGDNCSKNQDDCKDHQCQNGAQCVDEINSYACLCAEGYSGQLCEIPPAPRNSCEGTECQNGANCVDQGSRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKPGVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQCQDGYSGALCNQVGAVAEPCGGLQCLHGHCQASATRGAHCVCSPGFSGELCEQESECRGDPVRDFHRVQRGYAICQTTRPLSWVECRGACPGQGCCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGCAPCA

Target ORF information:

RefSeq Version	XM_017589694.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus slit guidance ligand 1 (Slit1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589694.1

ORF Insert Sequence:

ATGCCCAAGC TTCGGACCTT CCGGCTGCAC TCCAACCACC TGTTCTGTGA CTGTCACCTG 
GCCTGGCTCT CGCAGTGGCT GAGACAGAGG CCCACCATCG GGCTCTTCAC CCAGTGCTCT 
GGGCCCGCCA GCCTTCGGGG CCTCAATGTG GCAGAGGTGC AAAAGAGTGA GTTCAGCTGC 
TCAGGCCAGG GGGAGGCTGC ACAAGTACCT GCCTGCACCC TCTCATCCGG TTCCTGCCCA 
GCCATGTGCA GCTGTAGCAA TGGCATCGTG GACTGCCGTG GCAAAGGCCT CACTGCCATC 
CCTGCTAACC TTCCGGAGAC GATGACCGAG ATCCGCCTGG AGCTGAACGG TATCAAATCC 
ATCCCACCAG GAGCCTTCTC TCCCTACAGA AAGCTGCGCA GGATAGACCT GAGTAACAAC 
CAGATTGCTG AGATTGCACC CGATGCCTTC CAAGGCCTCC GCTCCTTGAA TTCCCTGGTC 
CTCTATGGCA ACAAGATCAC AGACTTGCCC CGAGGTGTAT TTGGAGGCCT GTACACCCTC 
CAGCTGCTGC TTCTGAATGC CAACAAGATC AACTGCATCC GGCCTGATGC CTTCCAGGAC 
CTGCAGAACC TCTCTCTGCT CTCCCTATAT GACAACAAGA TCCAGAGTCT CGCCAAGGGC 
ACCTTCACCT CCCTGCGGGC CATCCAGACA CTGCATCTGG CCCAGAACCC TTTCATCTGT 
GACTGTAACC TCAAGTGGCT AGCAGACTTC TTACGTACCA ACCCCATTGA GACAACTGGG 
GCCCGCTGTG CCAGCCCCCG GCGCCTTGCC AACAAGCGTA TCGGGCAGAT CAAAAGCAAG 
AAGTTCCGGT GCTCAGCCAA AGAGCAGTAC TTCATTCCAG GGACGGAGGA TTACCACCTG 
AACAGCGAGT GTACGAGTGA CGTGGCCTGT CCCCACAAGT GCCGCTGTGA GGCCAGTGTG 
GTGGAGTGCT CTGGCCTGAA GCTCTCTAAG ATCCCTGAGC GAATCCCCCA GTCCACGACT 
GAGCTACGAC TGAACAACAA TGAGATTTCA ATCCTAGAGG CCACTGGGTT GTTTAAGAAG 
CTCTCACATC TGAAGAAAAT AAACCTGAGC AACAACAAGG TGTCAGAGAT TGAAGACGGG 
ACGTTTGAAG GCGCCACCTC CGTCAGCGAG CTGCACCTGA CCGCCAACCA GCTGGAGTCT 
GTCAGAAGCG GCATGTTCCG GGGTCTAGAT GGCTTGCGGA CCCTGATGCT GAGGAACAAC 
CGTATCAGCT GCATCCACAA TGACAGCTTC ACTGGGCTGC GTAACGTGCG GCTCCTGTCT 
CTTTACGACA ACCACATCAC CACCATCTCC CCTGGAGCCT TCGACACCCT CCAGGCCCTG 
TCCACACTAA ACCTCCTGGC CAACCCATTC AACTGCAACT GCCAGTTGGC CTGGCTGGGG 
GACTGGCTTC GGAAGAGGAA AATTGTGACA GGGAACCCTC GCTGTCAGAA CCCAGACTTC 
CTGCGGCAGA TTCCTCTGCA AGATGTGGCC TTCCCTGACT TCAGGTGTGA GGAAGGCCAG 
GAGGAGGTGG GCTGCCTGCC CCGCCCACAG TGCCCCCAAG AGTGCGCCTG CCTGGACACC 
GTGGTCCGGT GTAGCAACAA GCACCTCCAG GCTCTGCCCA AGGGCATCCC CAAGAATGTC 
ACAGAACTCT ACTTAGATGG GAACCAGTTC ACACTGGTTC CGGGACAGCT GTCTACCTTC 
AAGTATCTGC AGCTTGTCGA CCTGAGCAAC AACAAGATCA GCTCCTTAAG CAACTCTTCC 
TTCACCAACA TGAGTCAGCT GACCACACTG ATCCTCAGCT ACAACGCCCT CCAGTGCATC 
CCCCCTCTGG CCTTCCAAGG ACTTCGTTCA CTGCGCTTGC TGTCTTTGCA TGGCAATGAT 
GTCTCCACCC TCCAAGAGGG AATCTTTGCA GACGTGACGT CTCTGTCTCA CCTGGCCATT 
GGTGCCAACC CCCTGTACGA CTGCCACCTC CGATGGCTCT CCAGCTGGGT GAAGACTGGC 
TACAAGGAAC CTGGCATTGC CCGCTGTGCT GGACCCCCAG AGATGGAGGG CAAACTGTTG 
CTCACCACAC CTGCCAAGAA GTTTGAATGC CAAGGTCCCC CCAGCCTGGC GGTTCAGGCC 
AAGTGTGATC CCTGCTTGTC CAGCCCATGC CAGAATCAGG GCACTTGTCA CAATGACCCT 
CTTGAGGTAT ACAGGTGTAC CTGCCCCAGT GGCTACAAGG GCCGAAACTG TGAGGTGTCT 
CTGGACAGCT GTTCCAGCAA CCCATGTGGA AATGGGGGAA CCTGCCATGC CCAGGAGGGC 
GAGGATGCTG GCTTCACGTG CTCCTGTCCT TCCGGCTTTG AAGGGCTGAC GTGTGGAATG 
AACACAGATG ATTGTGTGAA GCACGACTGT GTCAATGGGG GTGTCTGTGT GGATGGCATA 
GGGAATTACA CATGTCAGTG CCCCCTGCAG TACACAGGAA GGGCCTGTGA ACAGCTAGTG 
GACTTCTGCT CTCCGGATCT GAACCCATGT CAGCATGAGG CCCAATGTGT TGGCACCCCA 
GAGGGGCCCA GGTGTGAGTG TGTGCCAGGC TACACGGGTG ACAATTGCAG CAAAAACCAG 
GACGACTGCA AGGACCACCA GTGTCAGAAC GGGGCTCAGT GTGTGGATGA GATCAACAGC 
TACGCCTGTC TCTGTGCTGA GGGCTACAGC GGACAGCTCT GTGAGATCCC ACCTGCCCCC 
AGGAACTCCT GTGAAGGGAC TGAGTGCCAG AATGGAGCCA ACTGTGTAGA CCAGGGCAGC 
CGGCCCGTAT GCCAGTGCCT ACCAGGCTTT GGAGGTCCCG AATGTGAGAA GTTGCTCAGT 
GTCAACTTTG TGGACCGGGA CACTTACCTG CAGTTCACTG ACCTGCAGAA CTGGCCTCGG 
GCCAACATCA CTCTTCAGGT CTCCACAGCA GAGGACAATG GGATCCTCCT CTACAATGGG 
GATAATGACC ACATTGCAGT TGAGCTGTAC CAGGGCCATG TCCGTGTTAG CTACGACCCA 
GGCAGCTACC CCAGCTCTGC TATCTACAGT GCTGAAACAA TCAACGATGG GCAGTTCCAC 
ACAGTTGAGC TGGTGACCTT TGACCAGATG GTGAACCTCT CCATTGATGG TGGCAGCCCC 
ATGACCATGG ACAACTTTGG AAAGCACTAC ACACTCAACA GTGAGGCCCC CCTCTATGTG 
GGAGGGATGC CCGTGGATGT GAACTCAGCT GCCTTCCGCC TGTGGCAGAT CCTCAATGGC 
ACCAGCTTCC ACGGTTGCAT CCGGAATCTA TACATCAACA ACGAACTGCA GGACTTCACC 
AAGACACAGA TGAAGCCGGG CGTGGTGCCC GGCTGCGAGC CCTGCCGAAA ACTCTACTGT 
CTACATGGCA TTTGCCAGCC CAACGCCACC CCAGGGCCCG TGTGCCACTG CGAGGCTGGC 
TGGGGGGGCC TGCACTGTGA CCAGCCAGTG GACGGCCCCT GCCATGGCCA CAAGTGTGTC 
CATGGGAAAT GCGTGCCCCT CGATGCACTT GCCTACAGCT GCCAATGCCA GGATGGATAC 
TCGGGGGCTC TGTGCAACCA GGTCGGGGCT GTGGCAGAGC CCTGTGGGGG CTTACAGTGC 
CTGCATGGTC ACTGCCAGGC CTCGGCCACC AGAGGGGCAC ACTGTGTGTG CAGCCCAGGT 
TTTTCAGGCG AGCTGTGTGA GCAAGAGTCC GAGTGCCGGG GGGACCCTGT CCGGGACTTT 
CACCGGGTCC AGAGGGGCTA TGCCATCTGC CAGACCACGC GCCCACTGTC ATGGGTGGAA 
TGCCGGGGCG CGTGCCCGGG CCAGGGCTGC TGCCAGGGCC TGCGGCTGAA GCGGAGGAAG 
CTCACCTTCG AGTGCAGCGA TGGGACCTCG TTTGCTGAGG AGGTGGAGAA GCCCACCAAG 
TGCGGCTGTG CCCCGTGTGC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit1 ( NM_022953.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_075242.1 Rattus norvegicus slit guidance ligand 1 (Slit1), mRNA.

Price & Availability↑

CloneID	ORa06527
Clone ID Related Accession (Same CDS sequence)	NM_022953.2
Accession Version	NM_022953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	slit homolog 1 protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB011530.1, EV772160.1 and BF410201.1. On Oct 17, 2007 this sequence version replaced NM_022953.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB011530.1, AF133730.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCAGA GCTGTGGCTG 
CTGCTATGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CAGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAATGGT AACAATATCA CTCGGATCCA TAAGAATGAC 
TTTGCTGGGC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT TGGAGCTGTG 
GAACGGGGAG CTTTTGATGA CATGAAGGAG TTGGAGCGGC TACGACTGAA CCGCAACCAG 
CTGCAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTGTCCAG ACTGGACCTG 
AGTGAGAATT CTCTCCAGGC TGTGCCCAGA AAGGCTTTCC GAGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCAGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGTCACCTGG CCTGGCTCTC GCAGTGGCTG AGACAGAGGC CCACCATCGG GCTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAATGTGG CAGAGGTGCA AAAGAGTGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCA CAAGTACCTG CCTGCACCCT CTCATCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAAT GGCATCGTGG ACTGCCGTGG CAAAGGCCTC 
ACTGCCATCC CTGCTAACCT TCCGGAGACG ATGACCGAGA TCCGCCTGGA GCTGAACGGT 
ATCAAATCCA TCCCACCAGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGTAACAACC AGATTGCTGA GATTGCACCC GATGCCTTCC AAGGCCTCCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATCACA GACTTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACCCTCC AGCTGCTGCT TCTGAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTATATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTGCGGGCC ATCCAGACAC TGCATCTGGC CCAGAACCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATTGAG 
ACAACTGGGG CCCGCTGTGC CAGCCCCCGG CGCCTTGCCA ACAAGCGTAT CGGGCAGATC 
AAAAGCAAGA AGTTCCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACGGAGGAT 
TACCACCTGA ACAGCGAGTG TACGAGTGAC GTGGCCTGTC CCCACAAGTG CCGCTGTGAG 
GCCAGTGTGG TGGAGTGCTC TGGCCTGAAG CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCAA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ACAACAAGGT GTCAGAGATT 
GAAGACGGGA CGTTTGAAGG CGCCACCTCC GTCAGCGAGC TGCACCTGAC CGCCAACCAG 
CTGGAGTCTG TCAGAAGCGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG CATCCACAAT GACAGCTTCA CTGGGCTGCG TAACGTGCGG 
CTCCTGTCTC TTTACGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT CGACACCCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCATTCA ACTGCAACTG CCAGTTGGCC 
TGGCTGGGGG ACTGGCTTCG GAAGAGGAAA ATTGTGACAG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAA GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAAGA GTGCGCCTGC 
CTGGACACCG TGGTCCGGTG TAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA CTTGGATGGG AACCAGTTCA CACTGGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTTGTCGAC CTGAGCAACA ACAAGATCAG CTCCTTAAGC 
AACTCTTCCT TCACCAACAT GAGTCAGCTG ACCACACTGA TCCTCAGCTA CAACGCCCTC 
CAGTGCATCC CCCCTCTGGC CTTCCAAGGA CTTCGTTCAC TGCGCTTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGA ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCACC TCCGATGGCT CTCCAGCTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TGCTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCGGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAATCA GGGCACTTGT 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGAAAC 
TGTGAGGTGT CTCTGGACAG CTGTTCCAGC AACCCATGTG GAAATGGGGG AACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CTTCCGGCTT TGAAGGGCTG 
ACGTGTGGAA TGAACACAGA TGATTGTGTG AAGCACGACT GTGTCAATGG GGGTGTCTGT 
GTGGATGGCA TAGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAACAGCTAG TGGACTTCTG CTCTCCGGAT CTGAACCCAT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CAGAGGGGCC CAGGTGTGAG TGTGTGCCAG GCTACACGGG TGACAATTGC 
AGCAAAAACC AGGACGACTG CAAGGACCAC CAGTGTCAGA ACGGGGCTCA GTGTGTGGAT 
GAGATCAACA GCTACGCCTG TCTCTGTGCT GAGGGCTACA GCGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAACTC CTGTGAAGGG ACTGAGTGCC AGAATGGAGC CAACTGTGTA 
GACCAGGGCA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGAGGTCC CGAATGTGAG 
AAGTTGCTCA GTGTCAACTT TGTGGACCGG GACACTTACC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATCCTC 
CTCTACAATG GGGATAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGCTACGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GTGCTGAAAC AATCAACGAT 
GGGCAGTTCC ACACAGTTGA GCTGGTGACC TTTGACCAGA TGGTGAACCT CTCCATTGAT 
GGTGGCAGCC CCATGACCAT GGACAACTTT GGAAAGCACT ACACACTCAA CAGTGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCCGTGGAT GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GCACCAGCTT CCACGGTTGC ATCCGGAATC TATACATCAA CAACGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCG GGCGTGGTGC CCGGCTGCGA GCCCTGCCGA 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC CGTGTGCCAC 
TGCGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGACGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCC CTCGATGCAC TTGCCTACAG CTGCCAATGC 
CAGGATGGAT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTACAGT GCCTGCATGG TCACTGCCAG GCCTCGGCCA CCAGAGGGGC ACACTGTGTG 
TGCAGCCCAG GTTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC GCGCCCACTG 
TCATGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACCT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGCGGCTG TGCCCCGTGT GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075242.1
CDS	160..4755
Translation	MALTPQRGSSSGLSRPELWLLLWAAAWRLGATACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENSLQAVPRKAFRGATDLKNLQLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAAQVPACTLSSGSCPAMCSCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSGLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGATSVSELHLTANQLESVRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCQLAWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYCDCHLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFECQGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRNCEVSLDSCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGLTCGMNTDDCVKHDCVNGGVCVDGIGNYTCQCPLQYTGRACEQLVDFCSPDLNPCQHEAQCVGTPEGPRCECVPGYTGDNCSKNQDDCKDHQCQNGAQCVDEINSYACLCAEGYSGQLCEIPPAPRNSCEGTECQNGANCVDQGSRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKPGVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQCQDGYSGALCNQVGAVAEPCGGLQCLHGHCQASATRGAHCVCSPGFSGELCEQESECRGDPVRDFHRVQRGYAICQTTRPLSWVECRGACPGQGCCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGCAPCA

Target ORF information:

RefSeq Version	NM_022953.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus slit guidance ligand 1 (Slit1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022953.2

ORF Insert Sequence:

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCAGA GCTGTGGCTG 
CTGCTATGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CAGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAATGGT AACAATATCA CTCGGATCCA TAAGAATGAC 
TTTGCTGGGC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT TGGAGCTGTG 
GAACGGGGAG CTTTTGATGA CATGAAGGAG TTGGAGCGGC TACGACTGAA CCGCAACCAG 
CTGCAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTGTCCAG ACTGGACCTG 
AGTGAGAATT CTCTCCAGGC TGTGCCCAGA AAGGCTTTCC GAGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCAGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGTCACCTGG CCTGGCTCTC GCAGTGGCTG AGACAGAGGC CCACCATCGG GCTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAATGTGG CAGAGGTGCA AAAGAGTGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCA CAAGTACCTG CCTGCACCCT CTCATCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAAT GGCATCGTGG ACTGCCGTGG CAAAGGCCTC 
ACTGCCATCC CTGCTAACCT TCCGGAGACG ATGACCGAGA TCCGCCTGGA GCTGAACGGT 
ATCAAATCCA TCCCACCAGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGTAACAACC AGATTGCTGA GATTGCACCC GATGCCTTCC AAGGCCTCCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATCACA GACTTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACCCTCC AGCTGCTGCT TCTGAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTATATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTGCGGGCC ATCCAGACAC TGCATCTGGC CCAGAACCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATTGAG 
ACAACTGGGG CCCGCTGTGC CAGCCCCCGG CGCCTTGCCA ACAAGCGTAT CGGGCAGATC 
AAAAGCAAGA AGTTCCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACGGAGGAT 
TACCACCTGA ACAGCGAGTG TACGAGTGAC GTGGCCTGTC CCCACAAGTG CCGCTGTGAG 
GCCAGTGTGG TGGAGTGCTC TGGCCTGAAG CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCAA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ACAACAAGGT GTCAGAGATT 
GAAGACGGGA CGTTTGAAGG CGCCACCTCC GTCAGCGAGC TGCACCTGAC CGCCAACCAG 
CTGGAGTCTG TCAGAAGCGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG CATCCACAAT GACAGCTTCA CTGGGCTGCG TAACGTGCGG 
CTCCTGTCTC TTTACGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT CGACACCCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCATTCA ACTGCAACTG CCAGTTGGCC 
TGGCTGGGGG ACTGGCTTCG GAAGAGGAAA ATTGTGACAG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAA GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAAGA GTGCGCCTGC 
CTGGACACCG TGGTCCGGTG TAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA CTTGGATGGG AACCAGTTCA CACTGGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTTGTCGAC CTGAGCAACA ACAAGATCAG CTCCTTAAGC 
AACTCTTCCT TCACCAACAT GAGTCAGCTG ACCACACTGA TCCTCAGCTA CAACGCCCTC 
CAGTGCATCC CCCCTCTGGC CTTCCAAGGA CTTCGTTCAC TGCGCTTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGA ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCACC TCCGATGGCT CTCCAGCTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TGCTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCGGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAATCA GGGCACTTGT 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGAAAC 
TGTGAGGTGT CTCTGGACAG CTGTTCCAGC AACCCATGTG GAAATGGGGG AACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CTTCCGGCTT TGAAGGGCTG 
ACGTGTGGAA TGAACACAGA TGATTGTGTG AAGCACGACT GTGTCAATGG GGGTGTCTGT 
GTGGATGGCA TAGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAACAGCTAG TGGACTTCTG CTCTCCGGAT CTGAACCCAT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CAGAGGGGCC CAGGTGTGAG TGTGTGCCAG GCTACACGGG TGACAATTGC 
AGCAAAAACC AGGACGACTG CAAGGACCAC CAGTGTCAGA ACGGGGCTCA GTGTGTGGAT 
GAGATCAACA GCTACGCCTG TCTCTGTGCT GAGGGCTACA GCGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAACTC CTGTGAAGGG ACTGAGTGCC AGAATGGAGC CAACTGTGTA 
GACCAGGGCA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGAGGTCC CGAATGTGAG 
AAGTTGCTCA GTGTCAACTT TGTGGACCGG GACACTTACC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATCCTC 
CTCTACAATG GGGATAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGCTACGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GTGCTGAAAC AATCAACGAT 
GGGCAGTTCC ACACAGTTGA GCTGGTGACC TTTGACCAGA TGGTGAACCT CTCCATTGAT 
GGTGGCAGCC CCATGACCAT GGACAACTTT GGAAAGCACT ACACACTCAA CAGTGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCCGTGGAT GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GCACCAGCTT CCACGGTTGC ATCCGGAATC TATACATCAA CAACGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCG GGCGTGGTGC CCGGCTGCGA GCCCTGCCGA 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC CGTGTGCCAC 
TGCGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGACGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCC CTCGATGCAC TTGCCTACAG CTGCCAATGC 
CAGGATGGAT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTACAGT GCCTGCATGG TCACTGCCAG GCCTCGGCCA CCAGAGGGGC ACACTGTGTG 
TGCAGCCCAG GTTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC GCGCCCACTG 
TCATGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACCT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGCGGCTG TGCCCCGTGT GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Slit1 promotes regenerative neurite outgrowth of adult dorsal root ganglion neurons in vitro via binding to the Robo receptor.
Journal of chemical neuroanatomy39(4)256-61(2010 Jul)
Zhang HY,Zheng LF,Yi XN,Chen ZB,He ZP,Zhao D,Zhang XF,Ma ZJ

mRNA expression changes of slit proteins following peripheral nerve injury in the rat model.
Journal of chemical neuroanatomy36(3-4)170-6(2008 Dec)
Fujiwara T,Kubo T,Koyama Y,Tomita K,Yano K,Tohyama M,Hosokawa K

Axonal growth regulation of fetal and embryonic stem cell-derived dopaminergic neurons by Netrin-1 and Slits.
Stem cells (Dayton, Ohio)24(11)2504-13(2006 Nov)
Lin L,Isacson O

Slit proteins regulate distinct aspects of retinal ganglion cell axon guidance within dorsal and ventral retina.
The Journal of neuroscience : the official journal of the Society for Neuroscience26(31)8082-91(2006 Aug)
Thompson H,Camand O,Barker D,Erskine L

Slits contribute to the guidance of retinal ganglion cell axons in the mammalian optic tract.
Developmental biology296(2)476-84(2006 Aug)
Thompson H,Barker D,Camand O,Erskine L

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Slit1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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