Hand2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hand2
Entrez Gene ID	64637
Full Name	heart and neural crest derivatives expressed 2
General protein information	Preferred Names heart and neural crest derivatives expressed 2 Names heart- and neural crest derivatives-expressed protein 2 dHand protein deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2 heart and neural crest derivatives expressed transcript 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p11
Summary	acts in complex with Gata4 to activate transcription of cardiac specific genes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022696.1	NP_073187.1	heart- and neural crest derivatives-expressed protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HAND2	XP_003310653.1
Homo sapiens (human)	HAND2	NP_068808.1
	HAND2	XP_001085842.1
Canis lupus familiaris (dog)	HAND2	XP_005635942.1
Xenopus tropicalis (tropical clawed frog)	hand2	NP_001093695.1
Bos taurus (cattle)	HAND2	XP_003586406.1
Rattus norvegicus (Norway rat)	Hand2	NP_073187.1
Mus musculus (house mouse)	Hand2	NP_034532.3
Danio rerio (zebrafish)	hand2	NP_571701.2

Gene Ontology
Bibliography

Related articles in PubMed

Nfat and miR-25 cooperate to reactivate the transcription factor Hand2 in heart failure.
Dirkx E, Gladka MM, Philippen LE, Armand AS, Kinet V, Leptidis S, El Azzouzi H, Salic K, Bourajjaj M, da Silva GJ, Olieslagers S, van der Nagel R, de Weger R, Bitsch N, Kisters N, Seyen S, Morikawa Y, Chanoine C, Heymans S, Volders PG, Thum T, Dimmeler S, Cserjesi P, Eschenhagen T, da Costa Martins PA, De Windt LJ
Nature cell biology15(11)1282-93(2013 Nov)

Characterization of shear-sensitive genes in the normal rat aorta identifies Hand2 as a major flow-responsive transcription factor.
Bj?rck HM, Renner J, Maleki S, Nilsson SF, Kihlberg J, Folkersen L, Karlsson M, Ebbers T, Eriksson P, L?nne T
PloS one7(12)e52227(2012)

Hand2 loss-of-function in Hand1-expressing cells reveals distinct roles in epicardial and coronary vessel development.
Barnes RM, Firulli BA, VanDusen NJ, Morikawa Y, Conway SJ, Cserjesi P, Vincentz JW, Firulli AB
Circulation research108(8)940-9(2011 Apr)

Cytokines inhibit norepinephrine transporter expression by decreasing Hand2.
Pellegrino MJ, Parrish DC, Zigmond RE, Habecker BA
Molecular and cellular neurosciences46(3)671-80(2011 Mar)

Targeted deletion of Hand2 in cardiac neural crest-derived cells influences cardiac gene expression and outflow tract development.
Holler KL, Hendershot TJ, Troy SE, Vincentz JW, Firulli AB, Howard MJ
Developmental biology341(1)291-304(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HAND2 protein was endothelial specific and showed higher expression in the regions exposed to low WSS with disturbed flow.
Title: Characterization of shear-sensitive genes in the normal rat aorta identifies Hand2 as a major flow-responsive transcription factor.

These data suggest that cytokines can inhibit norepinephrine transporter expression through downregulation of Hand2 or Gata3 in cultured sympathetic neurons, but axotomy in adult animals selectively suppresses Hand2 expression.
Title: Cytokines inhibit norepinephrine transporter expression by decreasing Hand2.

Hand2 is necessary for terminal differentiatiion of enteric neurons from crest-derived precursors.
Title: Hand2 is necessary for terminal differentiation of enteric neurons from crest-derived precursors but not for their migration into the gut or for formation of glia.

expressed in the adult-rodent heart and is modulated during cardiac hypertrophy
Title: HAND1 and HAND2 are expressed in the adult-rodent heart and are modulated during cardiac hypertrophy.

interacts with GATA4 to synergistically activate cardiac gene expression through a p300-dependent mechanism
Title: The transcription factors GATA4 and dHAND physically interact to synergistically activate cardiac gene expression through a p300-dependent mechanism.

The following Hand2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hand2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06277	NM_022696.1 Latest version!	Rattus norvegicus heart and neural crest derivatives expressed 2 (Hand2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hand2 ( NM_022696.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073187.1 Rattus norvegicus heart and neural crest derivatives expressed 2 (Hand2), mRNA.

Price & Availability↑

CloneID	ORa06277
Clone ID Related Accession (Same CDS sequence)	NM_022696.1
Accession Version	NM_022696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Rattus norvegicus(Norway rat)
Product	heart- and neural crest derivatives-expressed protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y08138.1. On or before Aug 27, 2014 this sequence version replaced XM_008771238.1, XM_579461.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y08138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906351 [ECO:0000348] ##Evidence-Data-END##

ATGAGTCTGG TGGGGGGCTT TCCCCACCAC CCCGTGGTGC ACCATGAGGG CTACCCGTTC 
GCCGCAGCCG CAGCCGCCGC TGCTGCTGCC GCCGCCAGCC GCTGCAGTCA CGAGGAGAAC 
CCCTATTTCC ACGGCTGGCT TATTGGCCAC CCGGAGATGT CGCCCCCCGA CTACAGCATG 
GCCCTGTCCT ACAGTCCCGA GTACGCCAGC GGTGCCGCGG GCCTGGACCA CTCCCATTAT 
GGGGGAGTGC CGCCCGGTGC CGGGCCTCCC GGCCTGGGGG GGCCGCGCCC GGTGAAGCGT 
CGGGGCACCG CCAACCGCAA GGAGCGGCGC AGGACTCAGA GCATCAACAG CGCCTTCGCC 
GAGCTGCGCG AGTGCATCCC CAACGTGCCC GCCGACACCA AACTCTCCAA AATCAAGACT 
CTGCGCCTGG CCACCAGCTA CATCGCCTAC CTCATGGATC TGCTGGCCAA GGACGACCAG 
AACGGAGAGG CGGAGGCCTT CAAGGCGGAG ATCAAGAAGA CCGACGTGAA AGAGGAGAAG 
AGGAAGAAAG AGCTGAATGA AATCTTGAAA AGTACAGTGA GCAGCAACGA CAAGAAAACC 
AAAGGCCGGA CAGGCTGGCC ACAGCACGTC TGGGCCCTGG AGCTCAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073187.1
CDS	30..683
Translation	MSLVGGFPHHPVVHHEGYPFAAAAAAAAAAAASRCSHEENPYFHGWLIGHPEMSPPDYSMALSYSPEYASGAAGLDHSHYGGVPPGAGPPGLGGPRPVKRRGTANRKERRRTQSINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDLLAKDDQNGEAEAFKAEIKKTDVKEEKRKKELNEILKSTVSSNDKKTKGRTGWPQHVWALELKQ

Target ORF information:

RefSeq Version	NM_022696.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heart and neural crest derivatives expressed 2 (Hand2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022696.1

ORF Insert Sequence:

ATGAGTCTGG TGGGGGGCTT TCCCCACCAC CCCGTGGTGC ACCATGAGGG CTACCCGTTC 
GCCGCAGCCG CAGCCGCCGC TGCTGCTGCC GCCGCCAGCC GCTGCAGTCA CGAGGAGAAC 
CCCTATTTCC ACGGCTGGCT TATTGGCCAC CCGGAGATGT CGCCCCCCGA CTACAGCATG 
GCCCTGTCCT ACAGTCCCGA GTACGCCAGC GGTGCCGCGG GCCTGGACCA CTCCCATTAT 
GGGGGAGTGC CGCCCGGTGC CGGGCCTCCC GGCCTGGGGG GGCCGCGCCC GGTGAAGCGT 
CGGGGCACCG CCAACCGCAA GGAGCGGCGC AGGACTCAGA GCATCAACAG CGCCTTCGCC 
GAGCTGCGCG AGTGCATCCC CAACGTGCCC GCCGACACCA AACTCTCCAA AATCAAGACT 
CTGCGCCTGG CCACCAGCTA CATCGCCTAC CTCATGGATC TGCTGGCCAA GGACGACCAG 
AACGGAGAGG CGGAGGCCTT CAAGGCGGAG ATCAAGAAGA CCGACGTGAA AGAGGAGAAG 
AGGAAGAAAG AGCTGAATGA AATCTTGAAA AGTACAGTGA GCAGCAACGA CAAGAAAACC 
AAAGGCCGGA CAGGCTGGCC ACAGCACGTC TGGGCCCTGG AGCTCAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nfat and miR-25 cooperate to reactivate the transcription factor Hand2 in heart failure.
Nature cell biology15(11)1282-93(2013 Nov)
Dirkx E,Gladka MM,Philippen LE,Armand AS,Kinet V,Leptidis S,El Azzouzi H,Salic K,Bourajjaj M,da Silva GJ,Olieslagers S,van der Nagel R,de Weger R,Bitsch N,Kisters N,Seyen S,Morikawa Y,Chanoine C,Heymans S,Volders PG,Thum T,Dimmeler S,Cserjesi P,Eschenhagen T,da Costa Martins PA,De Windt LJ

Characterization of shear-sensitive genes in the normal rat aorta identifies Hand2 as a major flow-responsive transcription factor.
PloS one7(12)e52227(2012)
Bj?rck HM,Renner J,Maleki S,Nilsson SF,Kihlberg J,Folkersen L,Karlsson M,Ebbers T,Eriksson P,L?nne T

Hand2 loss-of-function in Hand1-expressing cells reveals distinct roles in epicardial and coronary vessel development.
Circulation research108(8)940-9(2011 Apr)
Barnes RM,Firulli BA,VanDusen NJ,Morikawa Y,Conway SJ,Cserjesi P,Vincentz JW,Firulli AB

Cytokines inhibit norepinephrine transporter expression by decreasing Hand2.
Molecular and cellular neurosciences46(3)671-80(2011 Mar)
Pellegrino MJ,Parrish DC,Zigmond RE,Habecker BA

Targeted deletion of Hand2 in cardiac neural crest-derived cells influences cardiac gene expression and outflow tract development.
Developmental biology341(1)291-304(2010 May)
Holler KL,Hendershot TJ,Troy SE,Vincentz JW,Firulli AB,Howard MJ