Nphs1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Nphs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nphs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa06172 NM_022628.1
Latest version!
Rattus norvegicus NPHS1 adhesion molecule, nephrin (Nphs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa43609 XM_008759186.2
Latest version!
Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa43610 XM_008759187.2
Latest version!
Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa06172
    Clone ID Related Accession (Same CDS sequence) NM_022628.1
    Accession Version NM_022628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Rattus norvegicus(Norway rat)
    Product nephrin precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF161715.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF161715.1, AF172255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642745, SAMN02642747 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGCTA AGAGAGTCAC TGTCAGAGGT GCCCGGACAA GCCCAATACA CAGAACGTCC 
    AGTTTGACTC CCCTGCTGCT CATGGGAATG CTGACCTCAG GCCTGGCCGA GTCGCCAGTC
    CCCACCTCAG CACCTCAAGG CTTCTGGGCT CTGTCTGAAA ACCTGACTGC GGTGGAAGGG
    ACAACAGTTA AGCTATGGTG CGGTGTCAGG GCCCCTGGCA GTGTGGTGCA GTGGGCTAAG
    GATGGGCTGC TTCTGGGTCC AAACCCGAAG ATGCCAGGCT TCCCGAGGTA CAGCCTGGAA
    GGAGATCGTG CTAAAGGCGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGTGATGAC
    GCAGAGTATG AATGCCAAGT CGGCCGCTCA GAGTTGGGTC CCGAGCTTGT GTCTCCTAAA
    GTAATCCTCT CCATTCTAGT TTCCCCCAAG GTGCTTCTGT TGACCCCCGA GGCAGGAAGC
    ACAGTGACCT GGGTAGCTGG GCAGGAGTAT GTGGTCACCT GTGTGTCTGG AGATGCAAAA
    CCAGCACCTG ACATCACCTT CATCCAGAGT GGACGAACTA TATTGGACGT CTCCTCCAAT
    GTGAATGAGG GATCAGAGGA GAAACTCTGC ATCACAGAGG CCGAAGCCAG GGTGATACCC
    CAGAGCTCGG ATAACGGGCA GTTACTGGTC TGTGAGGGTT CCAACCCAGC TTTGGACACT
    CCCATAAAGG CTTCATTCAC CATGAATATT CTGTTTCCCC CAGGACCTCC TGTCATTGAT
    TGGCCAGGCC TGAATGAGGG GCATGTGAGG GCAGGGGAGA ACCTGGAGCT GCCCTGCACA
    GCCAGAGGTG GCAATCCACC TGCTACCCTG CAGTGGCTGA AGAACGGTAA ACCAGTGTCC
    ACAGCCTGGG GCACCGAGCA TGCCCAGGCA GTGGCCCACA GCGTGCTGGT GATGACTGTA
    CGACCTGAAG ACCATGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGCGT GTCTGCAGGG
    ACCCAGGAGA GAAGCATCAC ACTACAGGTC ACCTTTCCCC CAAGCGCCAT TACCATCCTG
    GGATCTGTAT CACAATCGGA GAACAAGAAC GTGACCCTTT GCTGCCTGAC CAAGTCCAGT
    CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGCTGCTGCC CACAGATGAG
    ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTCAC ATTCTTGGTG
    CGGAGAGAAG ACAATGGCCT GCCCCTCACC TGTGAAGCCT TCAGTGACGC CTTCAGCAAG
    GAGACCTTCA AGAAGTCACT CACCTTGAAT GTGAAATACC CTGCCCAGAA GCTGTGGATT
    GAGGGGCCCC CAGAGGGACA GTACATCCGG ACTGGGACTC GGGTGAGGCT GGTATGCTTG
    GCCATCGGAG GCAACCCAGA CCCCTCCCTC ATCTGGTTTA AGGATTCACG TCCGGTGAGC
    GAGCCCCGGC AGCCCCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGG
    AGCACTTTCT CCCGCGAGCT GGTGTTGATC ATAGGTCCGC CGGACAACCG AGCCAAGTTC
    TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTT CCCCCCAACC
    AACCTGACCA TCCTGGCCAA CTCGTCCGCG CTGCGCCCAG GCGACGCCTT GAACTTGACC
    TGCGTCAGCA TCAGCAGCAA CCCCCCAGTC AACTTGTCTT GGGACAAGGA AGGAGAGAGG
    CTGGAAGATG TGGCTGCAAA ACCCCAGAGT GCACCGTTCA AAGGCTCCGC TGCATCCAGG
    AGTGTTTTTC TCAGAGTGTC ATCCCGAGAC CACGGTCAAC GGGTCACCTG CCGGGCCCAC
    AGCGAGGCAC TCCGTGAAAC CGTGAGCTCC TTCTACCGCT TCAATGTGCT GTATCCTCCA
    GAATTCCTGG GGGAGCAAGT CCGGGCAGTG ACCGTGGTGG AGCAGGGCCA GGTGCTGCTG
    CCGGTGTCGG TGTCCGCTAA CCCCGCCCCC GAGGCCTTCA ACTGGACCTT CCGAGGCTAC
    CGCCTCAGCC CAGCTGGGGG TCCCCGGCAC CGTATCCTGT CTGGAGGGGC TCTGCAGCTG
    TGGAATGTGA CCCGAGCTGA CGATGGCTTT TATCAGCTGC ACTGCCAGAA CTCAGAGGGC
    ACCGCTGAGG CGCTGTTGAA GCTGGACGTG CATTATGCTC CCACCATCCG TGCCCTCCGG
    GACCCTACTG AGGTGAATGT TGGGGGTTCT GTGGACATAG TCTGCACCGT TGACGCCAAT
    CCCATCCTCC CAGAGATGTT CAGCTGGGAG AGACTGGGAG AAGAAGAGGA GGATCTGAAC
    CTGGACGACA TGGAGAAAGT TTCCAAGGGA TCCACGGGGC GTCTGCGGAT TCGCCAAGCC
    AAGCTATCCC AGGCTGGTGC CTACCAGTGC ATCGTGGACA ATGGGGTGGC TCCTGCAGCC
    AGAGGACTGG TTCGTCTTGT CGTCCGATTT GCTCCCCAGG TGGATCAGCC TACTCCCCTA
    ACAAAAGTGG CTGCCGCTGG GGACAGCACC AGCTCAGCCA CACTGCACTG CCGTGCCCGG
    GGTGTCCCCA ACATCGACTT CACTTGGACC AAAAACGGGG TCCCTCTGGA TCTCCAAGAC
    CCCAGGTACA CAGAGCACAG GTACCACCAG GGTGTTGTCC ACAGCAGCCT CTTGACCATC
    GCTAATGTGT CTGCGGCCCA GGACTATGCC CTCTTCAAAT GCACGGCCAC CAATGCCCTT
    GGCTCTGACC ACACCAACAT CCAGCTCGTC AGCATCAGCC GCCCTGACCC TCCACTGGGA
    CTGAAGGTTG TCAGCATAAG CCCTCACTCG GTGGGGCTGG AGTGGAAGCC TGGCTTTGAT
    GGGGGTCTGC CTCAGAGGTT CCAAATCAGG TACGAGGCCC TCGAGACCCC AGGATTCCTC
    CACGTGGATG TCCTACCTAC ACAGGCCACT ACCTTCACGC TGACTGGGCT GAAGCCTTCT
    ACACGATATA GGATCTGGCT GTTGGCCAGC AATGCCCTGG GGGACAGTGG ATTGACGGAC
    AAGGGGATCC AGGTCTCCGT CACTACCCCA GGCCCCGACC AGGCTCCTGA AGACACAGAC
    CACCAGCTGC CCACAGAGCT GCCTCCAGGA CCCCCAAGGC TGCCCCTGCT GCCTGTGCTC
    TTTGCAGTTG GTGGTCTTCT GCTGCTCTCC AATGCCTCCT GTGTTGGGGG TCTCCTCTGG
    CGGAGAAGAC TGAGGCGCCT TGCTGAGGAG ATCTCAGAGA AGACAGAGGC AGGGTCGGAG
    GACAGGATCA GGAATGAATA TGAGGAGAGT CAGTGGACTG GGGACCGGGA CACGAGAAGC
    TCCACGGTTA GCACAGCAGA AGTGGACCCA AATTACTACT CCATGAGGGA CTTCAGCCCC
    CAGCTTCCCC CAACACTGGA GGAGGTGCTG TATCACCAAG GTGCTGAAGG CGAGGACATG
    GCCTTCCCCG GACACCTGCA TGATGAAGTG GAGAGAGCCT ATGGCCCGCC TGGGGCCTGG
    GGACCCCTCT ATGATGAAGT ACGAATGGAC CCCTATGACC TTCGCTGGCC TGAGGTCCAG
    TGTGAGGATC CGAGGGGAAT CTACGACCAG GTGGCAGCAG ACATGGATGC TGTGGAAGCT
    AGCTCTCTGC CGTTTGAGCT GAGGGGACAT CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_072150.1
    CDS404..4162
    Translation

    Target ORF information:

    RefSeq Version NM_022628.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus NPHS1 adhesion molecule, nephrin (Nphs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGCGCTA AGAGAGTCAC TGTCAGAGGT GCCCGGACAA GCCCAATACA CAGAACGTCC 
    AGTTTGACTC CCCTGCTGCT CATGGGAATG CTGACCTCAG GCCTGGCCGA GTCGCCAGTC
    CCCACCTCAG CACCTCAAGG CTTCTGGGCT CTGTCTGAAA ACCTGACTGC GGTGGAAGGG
    ACAACAGTTA AGCTATGGTG CGGTGTCAGG GCCCCTGGCA GTGTGGTGCA GTGGGCTAAG
    GATGGGCTGC TTCTGGGTCC AAACCCGAAG ATGCCAGGCT TCCCGAGGTA CAGCCTGGAA
    GGAGATCGTG CTAAAGGCGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGTGATGAC
    GCAGAGTATG AATGCCAAGT CGGCCGCTCA GAGTTGGGTC CCGAGCTTGT GTCTCCTAAA
    GTAATCCTCT CCATTCTAGT TTCCCCCAAG GTGCTTCTGT TGACCCCCGA GGCAGGAAGC
    ACAGTGACCT GGGTAGCTGG GCAGGAGTAT GTGGTCACCT GTGTGTCTGG AGATGCAAAA
    CCAGCACCTG ACATCACCTT CATCCAGAGT GGACGAACTA TATTGGACGT CTCCTCCAAT
    GTGAATGAGG GATCAGAGGA GAAACTCTGC ATCACAGAGG CCGAAGCCAG GGTGATACCC
    CAGAGCTCGG ATAACGGGCA GTTACTGGTC TGTGAGGGTT CCAACCCAGC TTTGGACACT
    CCCATAAAGG CTTCATTCAC CATGAATATT CTGTTTCCCC CAGGACCTCC TGTCATTGAT
    TGGCCAGGCC TGAATGAGGG GCATGTGAGG GCAGGGGAGA ACCTGGAGCT GCCCTGCACA
    GCCAGAGGTG GCAATCCACC TGCTACCCTG CAGTGGCTGA AGAACGGTAA ACCAGTGTCC
    ACAGCCTGGG GCACCGAGCA TGCCCAGGCA GTGGCCCACA GCGTGCTGGT GATGACTGTA
    CGACCTGAAG ACCATGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGCGT GTCTGCAGGG
    ACCCAGGAGA GAAGCATCAC ACTACAGGTC ACCTTTCCCC CAAGCGCCAT TACCATCCTG
    GGATCTGTAT CACAATCGGA GAACAAGAAC GTGACCCTTT GCTGCCTGAC CAAGTCCAGT
    CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGCTGCTGCC CACAGATGAG
    ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTCAC ATTCTTGGTG
    CGGAGAGAAG ACAATGGCCT GCCCCTCACC TGTGAAGCCT TCAGTGACGC CTTCAGCAAG
    GAGACCTTCA AGAAGTCACT CACCTTGAAT GTGAAATACC CTGCCCAGAA GCTGTGGATT
    GAGGGGCCCC CAGAGGGACA GTACATCCGG ACTGGGACTC GGGTGAGGCT GGTATGCTTG
    GCCATCGGAG GCAACCCAGA CCCCTCCCTC ATCTGGTTTA AGGATTCACG TCCGGTGAGC
    GAGCCCCGGC AGCCCCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGG
    AGCACTTTCT CCCGCGAGCT GGTGTTGATC ATAGGTCCGC CGGACAACCG AGCCAAGTTC
    TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTT CCCCCCAACC
    AACCTGACCA TCCTGGCCAA CTCGTCCGCG CTGCGCCCAG GCGACGCCTT GAACTTGACC
    TGCGTCAGCA TCAGCAGCAA CCCCCCAGTC AACTTGTCTT GGGACAAGGA AGGAGAGAGG
    CTGGAAGATG TGGCTGCAAA ACCCCAGAGT GCACCGTTCA AAGGCTCCGC TGCATCCAGG
    AGTGTTTTTC TCAGAGTGTC ATCCCGAGAC CACGGTCAAC GGGTCACCTG CCGGGCCCAC
    AGCGAGGCAC TCCGTGAAAC CGTGAGCTCC TTCTACCGCT TCAATGTGCT GTATCCTCCA
    GAATTCCTGG GGGAGCAAGT CCGGGCAGTG ACCGTGGTGG AGCAGGGCCA GGTGCTGCTG
    CCGGTGTCGG TGTCCGCTAA CCCCGCCCCC GAGGCCTTCA ACTGGACCTT CCGAGGCTAC
    CGCCTCAGCC CAGCTGGGGG TCCCCGGCAC CGTATCCTGT CTGGAGGGGC TCTGCAGCTG
    TGGAATGTGA CCCGAGCTGA CGATGGCTTT TATCAGCTGC ACTGCCAGAA CTCAGAGGGC
    ACCGCTGAGG CGCTGTTGAA GCTGGACGTG CATTATGCTC CCACCATCCG TGCCCTCCGG
    GACCCTACTG AGGTGAATGT TGGGGGTTCT GTGGACATAG TCTGCACCGT TGACGCCAAT
    CCCATCCTCC CAGAGATGTT CAGCTGGGAG AGACTGGGAG AAGAAGAGGA GGATCTGAAC
    CTGGACGACA TGGAGAAAGT TTCCAAGGGA TCCACGGGGC GTCTGCGGAT TCGCCAAGCC
    AAGCTATCCC AGGCTGGTGC CTACCAGTGC ATCGTGGACA ATGGGGTGGC TCCTGCAGCC
    AGAGGACTGG TTCGTCTTGT CGTCCGATTT GCTCCCCAGG TGGATCAGCC TACTCCCCTA
    ACAAAAGTGG CTGCCGCTGG GGACAGCACC AGCTCAGCCA CACTGCACTG CCGTGCCCGG
    GGTGTCCCCA ACATCGACTT CACTTGGACC AAAAACGGGG TCCCTCTGGA TCTCCAAGAC
    CCCAGGTACA CAGAGCACAG GTACCACCAG GGTGTTGTCC ACAGCAGCCT CTTGACCATC
    GCTAATGTGT CTGCGGCCCA GGACTATGCC CTCTTCAAAT GCACGGCCAC CAATGCCCTT
    GGCTCTGACC ACACCAACAT CCAGCTCGTC AGCATCAGCC GCCCTGACCC TCCACTGGGA
    CTGAAGGTTG TCAGCATAAG CCCTCACTCG GTGGGGCTGG AGTGGAAGCC TGGCTTTGAT
    GGGGGTCTGC CTCAGAGGTT CCAAATCAGG TACGAGGCCC TCGAGACCCC AGGATTCCTC
    CACGTGGATG TCCTACCTAC ACAGGCCACT ACCTTCACGC TGACTGGGCT GAAGCCTTCT
    ACACGATATA GGATCTGGCT GTTGGCCAGC AATGCCCTGG GGGACAGTGG ATTGACGGAC
    AAGGGGATCC AGGTCTCCGT CACTACCCCA GGCCCCGACC AGGCTCCTGA AGACACAGAC
    CACCAGCTGC CCACAGAGCT GCCTCCAGGA CCCCCAAGGC TGCCCCTGCT GCCTGTGCTC
    TTTGCAGTTG GTGGTCTTCT GCTGCTCTCC AATGCCTCCT GTGTTGGGGG TCTCCTCTGG
    CGGAGAAGAC TGAGGCGCCT TGCTGAGGAG ATCTCAGAGA AGACAGAGGC AGGGTCGGAG
    GACAGGATCA GGAATGAATA TGAGGAGAGT CAGTGGACTG GGGACCGGGA CACGAGAAGC
    TCCACGGTTA GCACAGCAGA AGTGGACCCA AATTACTACT CCATGAGGGA CTTCAGCCCC
    CAGCTTCCCC CAACACTGGA GGAGGTGCTG TATCACCAAG GTGCTGAAGG CGAGGACATG
    GCCTTCCCCG GACACCTGCA TGATGAAGTG GAGAGAGCCT ATGGCCCGCC TGGGGCCTGG
    GGACCCCTCT ATGATGAAGT ACGAATGGAC CCCTATGACC TTCGCTGGCC TGAGGTCCAG
    TGTGAGGATC CGAGGGGAAT CTACGACCAG GTGGCAGCAG ACATGGATGC TGTGGAAGCT
    AGCTCTCTGC CGTTTGAGCT GAGGGGACAT CTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43609
    Clone ID Related Accession (Same CDS sequence) XM_008759186.2
    Accession Version XM_008759186.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product nephrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGTGTC TGACGGCTTT GGCTTTCTGG GTTCATGGAA CTGCTGGACC CAGCACAGCT 
    GGCCTGGCCG AGTCGCCAGT CCCCACCTCA GCACCTCAAG GCTTCTGGGC TCTGTCTGAA
    AACCTGACTG CGGTGGAAGG GACAACAGTT AAGCTATGGT GCGGTGTCAG GGCCCCTGGC
    AGTGTGGTGC AGTGGGCTAA GGATGGGCTG CTTCTGGGTC CAAACCCGAA GATGCCAGGC
    TTCCCGAGGT ACAGCCTGGA AGGAGATCGT GCTAAAGGCG AGTTCCACCT GCTTATTGAA
    GCCTGTGACC TCAGTGATGA CGCAGAGTAT GAATGCCAAG TCGGCCGCTC AGAGTTGGGT
    CCCGAGCTTG TGTCTCCTAA AGTAATCCTC TCCATTCTAG TTTCCCCCAA GGTGCTTCTG
    TTGACCCCCG AGGCAGGAAG CACAGTGACC TGGGTAGCTG GGCAGGAGTA TGTGGTCACC
    TGTGTGTCTG GAGATGCAAA ACCAGCACCT GACATCACCT TCATCCAGAG TGGACGAACT
    ATATTGGACG TCTCCTCCAA TGTGAATGAG GGATCAGAGG AGAAACTCTG CATCACAGAG
    GCCGAAGCCA GGGTGATACC CCAGAGCTCG GATAACGGGC AGTTACTGGT CTGTGAGGGT
    TCCAACCCAG CTTTGGACAC TCCCATAAAG GCTTCATTCA CCATGAATAT TCTGTTTCCC
    CCAGGACCTC CTGTCATTGA TTGGCCAGGC CTGAATGAGG GGCATGTGAG GGCAGGGGAG
    AACCTGGAGC TGCCCTGCAC AGCCAGAGGT GGCAATCCAC CTGCTACCCT GCAGTGGCTG
    AAGAACGGTA AACCAGTGTC CACAGCCTGG GGCACCGAGC ATGCCCAGGC AGTGGCCCAC
    AGCGTGCTGG TGATGACTGT ACGACCTGAA GACCATGGAG CTCGGCTCAG CTGTCAGTCC
    TACAACAGCG TGTCTGCAGG GACCCAGGAG AGAAGCATCA CACTACAGGT CACCTTTCCC
    CCAAGCGCCA TTACCATCCT GGGATCTGTA TCACAATCGG AGAACAAGAA CGTGACCCTT
    TGCTGCCTGA CCAAGTCCAG TCGCCCACGG GTCCTGCTGC GATGGTGGTT GGGTGGACGG
    CAGCTGCTGC CCACAGATGA GACAGTCATG GATGGCCTGC ATGGTGGCCA CATCTCCATG
    TCCAATCTCA CATTCTTGGT GCGGAGAGAA GACAATGGCC TGCCCCTCAC CTGTGAAGCC
    TTCAGTGACG CCTTCAGCAA GGAGACCTTC AAGAAGTCAC TCACCTTGAA TGTGAAATAC
    CCTGCCCAGA AGCTGTGGAT TGAGGGGCCC CCAGAGGGAC AGTACATCCG GACTGGGACT
    CGGGTGAGGC TGGTATGCTT GGCCATCGGA GGCAACCCAG ACCCCTCCCT CATCTGGTTT
    AAGGATTCAC GTCCGGTGAG CGAGCCCCGG CAGCCCCAGG AGCCCCGGCG TGTGCAGCTG
    GGCAGTGTGG AGAAGTCCGG GAGCACTTTC TCCCGCGAGC TGGTGTTGAT CATAGGTCCG
    CCGGACAACC GAGCCAAGTT CTCCTGCAAG GCGGGTCAGC TCAGTGCGTC TACGCAGCTG
    GTGGTGCAGT TCCCCCCAAC CAACCTGACC ATCCTGGCCA ACTCGTCCGC GCTGCGCCCA
    GGCGACGCCT TGAACTTGAC CTGCGTCAGC ATCAGCAGCA ACCCCCCAGT CAACTTGTCT
    TGGGACAAGG AAGGAGAGAG GCTGGAAGAT GTGGCTGCAA AACCCCAGAG TGCACCGTTC
    AAAGGCTCCG CTGCATCCAG GAGTGTTTTT CTCAGAGTGT CATCCCGAGA CCACGGTCAA
    CGGGTCACCT GCCGGGCCCA CAGCGAGGCA CTCCGTGAAA CCGTGAGCTC CTTCTACCGC
    TTCAATGTGC TGTATCCTCC AGAATTCCTG GGGGAGCAAG TCCGGGCAGT GACCGTGGTG
    GAGCAGGGCC AGGTGCTGCT GCCGGTGTCG GTGTCCGCTA ACCCCGCCCC CGAGGCCTTC
    AACTGGACCT TCCGAGGCTA CCGCCTCAGC CCAGCTGGGG GTCCCCGGCA CCGTATCCTG
    TCTGGAGGGG CTCTGCAGCT GTGGAATGTG ACCCGAGCTG ACGATGGCTT TTATCAGCTG
    CACTGCCAGA ACTCAGAGGG CACCGCTGAG GCGCTGTTGA AGCTGGACGT GCATTATGCT
    CCCACCATCC GTGCCCTCCG GGACCCTACT GAGGTGAATG TTGGGGGTTC TGTGGACATA
    GTCTGCACCG TTGACGCCAA TCCCATCCTC CCAGAGATGT TCAGCTGGGA GAGACTGGGA
    GAAGAAGAGG AGGATCTGAA CCTGGACGAC ATGGAGAAAG TTTCCAAGGG ATCCACGGGG
    CGTCTGCGGA TTCGCCAAGC CAAGCTATCC CAGGCTGGTG CCTACCAGTG CATCGTGGAC
    AATGGGGTGG CTCCTGCAGC CAGAGGACTG GTTCGTCTTG TCGTCCGATT TGCTCCCCAG
    GTGGATCAGC CTACTCCCCT AACAAAAGTG GCTGCCGCTG GGGACAGCAC CAGCTCAGCC
    ACACTGCACT GCCGTGCCCG GGGTGTCCCC AACATCGACT TCACTTGGAC CAAAAACGGG
    GTCCCTCTGG ATCTCCAAGA CCCCAGGTAC ACAGAGCACA GGTACCACCA GGGTGTTGTC
    CACAGCAGCC TCTTGACCAT CGCTAATGTG TCTGCGGCCC AGGACTATGC CCTCTTCAAA
    TGCACGGCCA CCAATGCCCT TGGCTCTGAC CACACCAACA TCCAGCTCGT CAGCATCAGC
    CGCCCTGACC CTCCACTGGG ACTGAAGGTT GTCAGCATAA GCCCTCACTC GGTGGGGCTG
    GAGTGGAAGC CTGGCTTTGA TGGGGGTCTG CCTCAGAGGT TCCAAATCAG GTACGAGGCC
    CTCGAGACCC CAGGATTCCT CCACGTGGAT GTCCTACCTA CACAGGCCAC TACCTTCACG
    CTGACTGGGC TGAAGCCTTC TACACGATAT AGGATCTGGC TGTTGGCCAG CAATGCCCTG
    GGGGACAGTG GATTGACGGA CAAGGGGATC CAGGTCTCCG TCACTACCCC AGGCCCCGAC
    CAGGCTCCTG AAGACACAGA CCACCAGCTG CCCACAGAGC TGCCTCCAGG ACCCCCAAGG
    CTGCCCCTGC TGCCTGTGCT CTTTGCAGTT GGTGGTCTTC TGCTGCTCTC CAATGCCTCC
    TGTGTTGGGG GTCTCCTCTG GCGGAGAAGA CTGAGGCGCC TTGCTGAGGA GATCTCAGAG
    AAGACAGAGG CAGGGTCGGA GGACAGGATC AGGAATGAAT ATGAGGAGAG TCAGTGGACT
    GGGGACCGGG ACACGAGAAG CTCCACGGTT AGCACAGCAG AAGTGGACCC AAATTACTAC
    TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCAACACTGG AGGAGGTGCT GTATCACCAA
    GGTGCTGAAG GCGAGGACAT GGCCTTCCCC GGACACCTGC ATGATGAAGT GGAGAGAGCC
    TATGGCCCGC CTGGGGCCTG GGGACCCCTC TATGATGAAG TACGAATGGA CCCCTATGAC
    CTTCGCTGGC CTGAGGTCCA GTGTGAGGAT CCGAGGGGAA TCTACGACCA GGTGGCAGCA
    GACATGGATG CTGTGGAAGC TAGCTCTCTG CCGTTTGAGC TGAGGGGACA TCTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008757408.1
    CDS123..3842
    Translation

    Target ORF information:

    RefSeq Version XM_008759186.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008759186.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGTGTC TGACGGCTTT GGCTTTCTGG GTTCATGGAA CTGCTGGACC CAGCACAGCT 
    GGCCTGGCCG AGTCGCCAGT CCCCACCTCA GCACCTCAAG GCTTCTGGGC TCTGTCTGAA
    AACCTGACTG CGGTGGAAGG GACAACAGTT AAGCTATGGT GCGGTGTCAG GGCCCCTGGC
    AGTGTGGTGC AGTGGGCTAA GGATGGGCTG CTTCTGGGTC CAAACCCGAA GATGCCAGGC
    TTCCCGAGGT ACAGCCTGGA AGGAGATCGT GCTAAAGGCG AGTTCCACCT GCTTATTGAA
    GCCTGTGACC TCAGTGATGA CGCAGAGTAT GAATGCCAAG TCGGCCGCTC AGAGTTGGGT
    CCCGAGCTTG TGTCTCCTAA AGTAATCCTC TCCATTCTAG TTTCCCCCAA GGTGCTTCTG
    TTGACCCCCG AGGCAGGAAG CACAGTGACC TGGGTAGCTG GGCAGGAGTA TGTGGTCACC
    TGTGTGTCTG GAGATGCAAA ACCAGCACCT GACATCACCT TCATCCAGAG TGGACGAACT
    ATATTGGACG TCTCCTCCAA TGTGAATGAG GGATCAGAGG AGAAACTCTG CATCACAGAG
    GCCGAAGCCA GGGTGATACC CCAGAGCTCG GATAACGGGC AGTTACTGGT CTGTGAGGGT
    TCCAACCCAG CTTTGGACAC TCCCATAAAG GCTTCATTCA CCATGAATAT TCTGTTTCCC
    CCAGGACCTC CTGTCATTGA TTGGCCAGGC CTGAATGAGG GGCATGTGAG GGCAGGGGAG
    AACCTGGAGC TGCCCTGCAC AGCCAGAGGT GGCAATCCAC CTGCTACCCT GCAGTGGCTG
    AAGAACGGTA AACCAGTGTC CACAGCCTGG GGCACCGAGC ATGCCCAGGC AGTGGCCCAC
    AGCGTGCTGG TGATGACTGT ACGACCTGAA GACCATGGAG CTCGGCTCAG CTGTCAGTCC
    TACAACAGCG TGTCTGCAGG GACCCAGGAG AGAAGCATCA CACTACAGGT CACCTTTCCC
    CCAAGCGCCA TTACCATCCT GGGATCTGTA TCACAATCGG AGAACAAGAA CGTGACCCTT
    TGCTGCCTGA CCAAGTCCAG TCGCCCACGG GTCCTGCTGC GATGGTGGTT GGGTGGACGG
    CAGCTGCTGC CCACAGATGA GACAGTCATG GATGGCCTGC ATGGTGGCCA CATCTCCATG
    TCCAATCTCA CATTCTTGGT GCGGAGAGAA GACAATGGCC TGCCCCTCAC CTGTGAAGCC
    TTCAGTGACG CCTTCAGCAA GGAGACCTTC AAGAAGTCAC TCACCTTGAA TGTGAAATAC
    CCTGCCCAGA AGCTGTGGAT TGAGGGGCCC CCAGAGGGAC AGTACATCCG GACTGGGACT
    CGGGTGAGGC TGGTATGCTT GGCCATCGGA GGCAACCCAG ACCCCTCCCT CATCTGGTTT
    AAGGATTCAC GTCCGGTGAG CGAGCCCCGG CAGCCCCAGG AGCCCCGGCG TGTGCAGCTG
    GGCAGTGTGG AGAAGTCCGG GAGCACTTTC TCCCGCGAGC TGGTGTTGAT CATAGGTCCG
    CCGGACAACC GAGCCAAGTT CTCCTGCAAG GCGGGTCAGC TCAGTGCGTC TACGCAGCTG
    GTGGTGCAGT TCCCCCCAAC CAACCTGACC ATCCTGGCCA ACTCGTCCGC GCTGCGCCCA
    GGCGACGCCT TGAACTTGAC CTGCGTCAGC ATCAGCAGCA ACCCCCCAGT CAACTTGTCT
    TGGGACAAGG AAGGAGAGAG GCTGGAAGAT GTGGCTGCAA AACCCCAGAG TGCACCGTTC
    AAAGGCTCCG CTGCATCCAG GAGTGTTTTT CTCAGAGTGT CATCCCGAGA CCACGGTCAA
    CGGGTCACCT GCCGGGCCCA CAGCGAGGCA CTCCGTGAAA CCGTGAGCTC CTTCTACCGC
    TTCAATGTGC TGTATCCTCC AGAATTCCTG GGGGAGCAAG TCCGGGCAGT GACCGTGGTG
    GAGCAGGGCC AGGTGCTGCT GCCGGTGTCG GTGTCCGCTA ACCCCGCCCC CGAGGCCTTC
    AACTGGACCT TCCGAGGCTA CCGCCTCAGC CCAGCTGGGG GTCCCCGGCA CCGTATCCTG
    TCTGGAGGGG CTCTGCAGCT GTGGAATGTG ACCCGAGCTG ACGATGGCTT TTATCAGCTG
    CACTGCCAGA ACTCAGAGGG CACCGCTGAG GCGCTGTTGA AGCTGGACGT GCATTATGCT
    CCCACCATCC GTGCCCTCCG GGACCCTACT GAGGTGAATG TTGGGGGTTC TGTGGACATA
    GTCTGCACCG TTGACGCCAA TCCCATCCTC CCAGAGATGT TCAGCTGGGA GAGACTGGGA
    GAAGAAGAGG AGGATCTGAA CCTGGACGAC ATGGAGAAAG TTTCCAAGGG ATCCACGGGG
    CGTCTGCGGA TTCGCCAAGC CAAGCTATCC CAGGCTGGTG CCTACCAGTG CATCGTGGAC
    AATGGGGTGG CTCCTGCAGC CAGAGGACTG GTTCGTCTTG TCGTCCGATT TGCTCCCCAG
    GTGGATCAGC CTACTCCCCT AACAAAAGTG GCTGCCGCTG GGGACAGCAC CAGCTCAGCC
    ACACTGCACT GCCGTGCCCG GGGTGTCCCC AACATCGACT TCACTTGGAC CAAAAACGGG
    GTCCCTCTGG ATCTCCAAGA CCCCAGGTAC ACAGAGCACA GGTACCACCA GGGTGTTGTC
    CACAGCAGCC TCTTGACCAT CGCTAATGTG TCTGCGGCCC AGGACTATGC CCTCTTCAAA
    TGCACGGCCA CCAATGCCCT TGGCTCTGAC CACACCAACA TCCAGCTCGT CAGCATCAGC
    CGCCCTGACC CTCCACTGGG ACTGAAGGTT GTCAGCATAA GCCCTCACTC GGTGGGGCTG
    GAGTGGAAGC CTGGCTTTGA TGGGGGTCTG CCTCAGAGGT TCCAAATCAG GTACGAGGCC
    CTCGAGACCC CAGGATTCCT CCACGTGGAT GTCCTACCTA CACAGGCCAC TACCTTCACG
    CTGACTGGGC TGAAGCCTTC TACACGATAT AGGATCTGGC TGTTGGCCAG CAATGCCCTG
    GGGGACAGTG GATTGACGGA CAAGGGGATC CAGGTCTCCG TCACTACCCC AGGCCCCGAC
    CAGGCTCCTG AAGACACAGA CCACCAGCTG CCCACAGAGC TGCCTCCAGG ACCCCCAAGG
    CTGCCCCTGC TGCCTGTGCT CTTTGCAGTT GGTGGTCTTC TGCTGCTCTC CAATGCCTCC
    TGTGTTGGGG GTCTCCTCTG GCGGAGAAGA CTGAGGCGCC TTGCTGAGGA GATCTCAGAG
    AAGACAGAGG CAGGGTCGGA GGACAGGATC AGGAATGAAT ATGAGGAGAG TCAGTGGACT
    GGGGACCGGG ACACGAGAAG CTCCACGGTT AGCACAGCAG AAGTGGACCC AAATTACTAC
    TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCAACACTGG AGGAGGTGCT GTATCACCAA
    GGTGCTGAAG GCGAGGACAT GGCCTTCCCC GGACACCTGC ATGATGAAGT GGAGAGAGCC
    TATGGCCCGC CTGGGGCCTG GGGACCCCTC TATGATGAAG TACGAATGGA CCCCTATGAC
    CTTCGCTGGC CTGAGGTCCA GTGTGAGGAT CCGAGGGGAA TCTACGACCA GGTGGCAGCA
    GACATGGATG CTGTGGAAGC TAGCTCTCTG CCGTTTGAGC TGAGGGGACA TCTGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43610
    Clone ID Related Accession (Same CDS sequence) XM_008759187.2
    Accession Version XM_008759187.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product nephrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCGCTA AGAGAGTCAC TGTCAGAGGT GCCCGGACAA GCCCAATACA CAGAACGTCC 
    AGTTTGACTC CCCTGCTGCT CATGGGAATG CTGACCTCAG GCCTGGCCGA GTCGCCAGTC
    CCCACCTCAG CACCTCAAGG CTTCTGGGCT CTGTCTGAAA ACCTGACTGC GGTGGAAGGG
    ACAACAGTTA AGCTATGGTG CGGTGTCAGG GCCCCTGGCA GTGTGGTGCA GTGGGCTAAG
    GATGGGCTGC TTCTGGGTCC AAACCCGAAG ATGCCAGGCT TCCCGAGGTA CAGCCTGGAA
    GGAGATCGTG CTAAAGGCGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGTGATGAC
    GCAGAGTATG AATGCCAAGT CGGCCGCTCA GAGTTGGGTC CCGAGCTTGT GTCTCCTAAA
    GTAATCCTCT CCATTCTAGT TTCCCCCAAG GTGCTTCTGT TGACCCCCGA GGCAGGAAGC
    ACAGTGACCT GGGTAGCTGG GCAGGAGTAT GTGGTCACCT GTGTGTCTGG AGATGCAAAA
    CCAGCACCTG ACATCACCTT CATCCAGAGT GGACGAACTA TATTGGACGT CTCCTCCAAT
    GTGAATGAGG GATCAGAGGA GAAACTCTGC ATCACAGAGG CCGAAGCCAG GGTGATACCC
    CAGAGCTCGG ATAACGGGCA GTTACTGGTC TGTGAGGGTT CCAACCCAGC TTTGGACACT
    CCCATAAAGG CTTCATTCAC CATGAATATT CTGTTTCCCC CAGGACCTCC TGTCATTGAT
    TGGCCAGGCC TGAATGAGGG GCATGTGAGG GCAGGGGAGA ACCTGGAGCT GCCCTGCACA
    GCCAGAGGTG GCAATCCACC TGCTACCCTG CAGTGGCTGA AGAACGGTAA ACCAGTGTCC
    ACAGCCTGGG GCACCGAGCA TGCCCAGGCA GTGGCCCACA GCGTGCTGGT GATGACTGTA
    CGACCTGAAG ACCATGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGCGT GTCTGCAGGG
    ACCCAGGAGA GAAGCATCAC ACTACAGGTC ACCTTTCCCC CAAGCGCCAT TACCATCCTG
    GGATCTGTAT CACAATCGGA GAACAAGAAC GTGACCCTTT GCTGCCTGAC CAAGTCCAGT
    CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGCTGCTGCC CACAGATGAG
    ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTCAC ATTCTTGGTG
    CGGAGAGAAG ACAATGGCCT GCCCCTCACC TGTGAAGCCT TCAGTGACGC CTTCAGCAAG
    GAGACCTTCA AGAAGTCACT CACCTTGAAT GTGAAATACC CTGCCCAGAA GCTGTGGATT
    GAGGGGCCCC CAGAGGGACA GTACATCCGG ACTGGGACTC GGGTGAGGCT GGTATGCTTG
    GCCATCGGAG GCAACCCAGA CCCCTCCCTC ATCTGGTTTA AGGATTCACG TCCGGTGAGC
    GAGCCCCGGC AGCCCCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGG
    AGCACTTTCT CCCGCGAGCT GGTGTTGATC ATAGGTCCGC CGGACAACCG AGCCAAGTTC
    TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTG CGACGCCTTG
    AACTTGACCT GCGTCAGCAT CAGCAGCAAC CCCCCAGTCA ACTTGTCTTG GGACAAGGAA
    GGAGAGAGGC TGGAAGATGT GGCTGCAAAA CCCCAGAGTG CACCGTTCAA AGGCTCCGCT
    GCATCCAGGA GTGTTTTTCT CAGAGTGTCA TCCCGAGACC ACGGTCAACG GGTCACCTGC
    CGGGCCCACA GCGAGGCACT CCGTGAAACC GTGAGCTCCT TCTACCGCTT CAATGTGCTG
    TATCCTCCAG AATTCCTGGG GGAGCAAGTC CGGGCAGTGA CCGTGGTGGA GCAGGGCCAG
    GTGCTGCTGC CGGTGTCGGT GTCCGCTAAC CCCGCCCCCG AGGCCTTCAA CTGGACCTTC
    CGAGGCTACC GCCTCAGCCC AGCTGGGGGT CCCCGGCACC GTATCCTGTC TGGAGGGGCT
    CTGCAGCTGT GGAATGTGAC CCGAGCTGAC GATGGCTTTT ATCAGCTGCA CTGCCAGAAC
    TCAGAGGGCA CCGCTGAGGC GCTGTTGAAG CTGGACGTGC ATTATGCTCC CACCATCCGT
    GCCCTCCGGG ACCCTACTGA GGTGAATGTT GGGGGTTCTG TGGACATAGT CTGCACCGTT
    GACGCCAATC CCATCCTCCC AGAGATGTTC AGCTGGGAGA GACTGGGAGA AGAAGAGGAG
    GATCTGAACC TGGACGACAT GGAGAAAGTT TCCAAGGGAT CCACGGGGCG TCTGCGGATT
    CGCCAAGCCA AGCTATCCCA GGCTGGTGCC TACCAGTGCA TCGTGGACAA TGGGGTGGCT
    CCTGCAGCCA GAGGACTGGT TCGTCTTGTC GTCCGATTTG CTCCCCAGGT GGATCAGCCT
    ACTCCCCTAA CAAAAGTGGC TGCCGCTGGG GACAGCACCA GCTCAGCCAC ACTGCACTGC
    CGTGCCCGGG GTGTCCCCAA CATCGACTTC ACTTGGACCA AAAACGGGGT CCCTCTGGAT
    CTCCAAGACC CCAGGTACAC AGAGCACAGG TACCACCAGG GTGTTGTCCA CAGCAGCCTC
    TTGACCATCG CTAATGTGTC TGCGGCCCAG GACTATGCCC TCTTCAAATG CACGGCCACC
    AATGCCCTTG GCTCTGACCA CACCAACATC CAGCTCGTCA GCATCAGCCG CCCTGACCCT
    CCACTGGGAC TGAAGGTTGT CAGCATAAGC CCTCACTCGG TGGGGCTGGA GTGGAAGCCT
    GGCTTTGATG GGGGTCTGCC TCAGAGGTTC CAAATCAGGT ACGAGGCCCT CGAGACCCCA
    GGATTCCTCC ACGTGGATGT CCTACCTACA CAGGCCACTA CCTTCACGCT GACTGGGCTG
    AAGCCTTCTA CACGATATAG GATCTGGCTG TTGGCCAGCA ATGCCCTGGG GGACAGTGGA
    TTGACGGACA AGGGGATCCA GGTCTCCGTC ACTACCCCAG GCCCCGACCA GGCTCCTGAA
    GACACAGACC ACCAGCTGCC CACAGAGCTG CCTCCAGGAC CCCCAAGGCT GCCCCTGCTG
    CCTGTGCTCT TTGCAGTTGG TGGTCTTCTG CTGCTCTCCA ATGCCTCCTG TGTTGGGGGT
    CTCCTCTGGC GGAGAAGACT GAGGCGCCTT GCTGAGGAGA TCTCAGAGAA GACAGAGGCA
    GGGTCGGAGG ACAGGATCAG GAATGAATAT GAGGAGAGTC AGTGGACTGG GGACCGGGAC
    ACGAGAAGCT CCACGGTTAG CACAGCAGAA GTGGACCCAA ATTACTACTC CATGAGGGAC
    TTCAGCCCCC AGCTTCCCCC AACACTGGAG GAGGTGCTGT ATCACCAAGG TGCTGAAGGC
    GAGGACATGG CCTTCCCCGG ACACCTGCAT GATGAAGTGG AGAGAGCCTA TGGCCCGCCT
    GGGGCCTGGG GACCCCTCTA TGATGAAGTA CGAATGGACC CCTATGACCT TCGCTGGCCT
    GAGGTCCAGT GTGAGGATCC GAGGGGAATC TACGACCAGG TGGCAGCAGA CATGGATGCT
    GTGGAAGCTA GCTCTCTGCC GTTTGAGCTG AGGGGACATC TGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008757409.1
    CDS491..4198
    Translation

    Target ORF information:

    RefSeq Version XM_008759187.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008759187.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCGCTA AGAGAGTCAC TGTCAGAGGT GCCCGGACAA GCCCAATACA CAGAACGTCC 
    AGTTTGACTC CCCTGCTGCT CATGGGAATG CTGACCTCAG GCCTGGCCGA GTCGCCAGTC
    CCCACCTCAG CACCTCAAGG CTTCTGGGCT CTGTCTGAAA ACCTGACTGC GGTGGAAGGG
    ACAACAGTTA AGCTATGGTG CGGTGTCAGG GCCCCTGGCA GTGTGGTGCA GTGGGCTAAG
    GATGGGCTGC TTCTGGGTCC AAACCCGAAG ATGCCAGGCT TCCCGAGGTA CAGCCTGGAA
    GGAGATCGTG CTAAAGGCGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGTGATGAC
    GCAGAGTATG AATGCCAAGT CGGCCGCTCA GAGTTGGGTC CCGAGCTTGT GTCTCCTAAA
    GTAATCCTCT CCATTCTAGT TTCCCCCAAG GTGCTTCTGT TGACCCCCGA GGCAGGAAGC
    ACAGTGACCT GGGTAGCTGG GCAGGAGTAT GTGGTCACCT GTGTGTCTGG AGATGCAAAA
    CCAGCACCTG ACATCACCTT CATCCAGAGT GGACGAACTA TATTGGACGT CTCCTCCAAT
    GTGAATGAGG GATCAGAGGA GAAACTCTGC ATCACAGAGG CCGAAGCCAG GGTGATACCC
    CAGAGCTCGG ATAACGGGCA GTTACTGGTC TGTGAGGGTT CCAACCCAGC TTTGGACACT
    CCCATAAAGG CTTCATTCAC CATGAATATT CTGTTTCCCC CAGGACCTCC TGTCATTGAT
    TGGCCAGGCC TGAATGAGGG GCATGTGAGG GCAGGGGAGA ACCTGGAGCT GCCCTGCACA
    GCCAGAGGTG GCAATCCACC TGCTACCCTG CAGTGGCTGA AGAACGGTAA ACCAGTGTCC
    ACAGCCTGGG GCACCGAGCA TGCCCAGGCA GTGGCCCACA GCGTGCTGGT GATGACTGTA
    CGACCTGAAG ACCATGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGCGT GTCTGCAGGG
    ACCCAGGAGA GAAGCATCAC ACTACAGGTC ACCTTTCCCC CAAGCGCCAT TACCATCCTG
    GGATCTGTAT CACAATCGGA GAACAAGAAC GTGACCCTTT GCTGCCTGAC CAAGTCCAGT
    CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGCTGCTGCC CACAGATGAG
    ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTCAC ATTCTTGGTG
    CGGAGAGAAG ACAATGGCCT GCCCCTCACC TGTGAAGCCT TCAGTGACGC CTTCAGCAAG
    GAGACCTTCA AGAAGTCACT CACCTTGAAT GTGAAATACC CTGCCCAGAA GCTGTGGATT
    GAGGGGCCCC CAGAGGGACA GTACATCCGG ACTGGGACTC GGGTGAGGCT GGTATGCTTG
    GCCATCGGAG GCAACCCAGA CCCCTCCCTC ATCTGGTTTA AGGATTCACG TCCGGTGAGC
    GAGCCCCGGC AGCCCCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGG
    AGCACTTTCT CCCGCGAGCT GGTGTTGATC ATAGGTCCGC CGGACAACCG AGCCAAGTTC
    TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTG CGACGCCTTG
    AACTTGACCT GCGTCAGCAT CAGCAGCAAC CCCCCAGTCA ACTTGTCTTG GGACAAGGAA
    GGAGAGAGGC TGGAAGATGT GGCTGCAAAA CCCCAGAGTG CACCGTTCAA AGGCTCCGCT
    GCATCCAGGA GTGTTTTTCT CAGAGTGTCA TCCCGAGACC ACGGTCAACG GGTCACCTGC
    CGGGCCCACA GCGAGGCACT CCGTGAAACC GTGAGCTCCT TCTACCGCTT CAATGTGCTG
    TATCCTCCAG AATTCCTGGG GGAGCAAGTC CGGGCAGTGA CCGTGGTGGA GCAGGGCCAG
    GTGCTGCTGC CGGTGTCGGT GTCCGCTAAC CCCGCCCCCG AGGCCTTCAA CTGGACCTTC
    CGAGGCTACC GCCTCAGCCC AGCTGGGGGT CCCCGGCACC GTATCCTGTC TGGAGGGGCT
    CTGCAGCTGT GGAATGTGAC CCGAGCTGAC GATGGCTTTT ATCAGCTGCA CTGCCAGAAC
    TCAGAGGGCA CCGCTGAGGC GCTGTTGAAG CTGGACGTGC ATTATGCTCC CACCATCCGT
    GCCCTCCGGG ACCCTACTGA GGTGAATGTT GGGGGTTCTG TGGACATAGT CTGCACCGTT
    GACGCCAATC CCATCCTCCC AGAGATGTTC AGCTGGGAGA GACTGGGAGA AGAAGAGGAG
    GATCTGAACC TGGACGACAT GGAGAAAGTT TCCAAGGGAT CCACGGGGCG TCTGCGGATT
    CGCCAAGCCA AGCTATCCCA GGCTGGTGCC TACCAGTGCA TCGTGGACAA TGGGGTGGCT
    CCTGCAGCCA GAGGACTGGT TCGTCTTGTC GTCCGATTTG CTCCCCAGGT GGATCAGCCT
    ACTCCCCTAA CAAAAGTGGC TGCCGCTGGG GACAGCACCA GCTCAGCCAC ACTGCACTGC
    CGTGCCCGGG GTGTCCCCAA CATCGACTTC ACTTGGACCA AAAACGGGGT CCCTCTGGAT
    CTCCAAGACC CCAGGTACAC AGAGCACAGG TACCACCAGG GTGTTGTCCA CAGCAGCCTC
    TTGACCATCG CTAATGTGTC TGCGGCCCAG GACTATGCCC TCTTCAAATG CACGGCCACC
    AATGCCCTTG GCTCTGACCA CACCAACATC CAGCTCGTCA GCATCAGCCG CCCTGACCCT
    CCACTGGGAC TGAAGGTTGT CAGCATAAGC CCTCACTCGG TGGGGCTGGA GTGGAAGCCT
    GGCTTTGATG GGGGTCTGCC TCAGAGGTTC CAAATCAGGT ACGAGGCCCT CGAGACCCCA
    GGATTCCTCC ACGTGGATGT CCTACCTACA CAGGCCACTA CCTTCACGCT GACTGGGCTG
    AAGCCTTCTA CACGATATAG GATCTGGCTG TTGGCCAGCA ATGCCCTGGG GGACAGTGGA
    TTGACGGACA AGGGGATCCA GGTCTCCGTC ACTACCCCAG GCCCCGACCA GGCTCCTGAA
    GACACAGACC ACCAGCTGCC CACAGAGCTG CCTCCAGGAC CCCCAAGGCT GCCCCTGCTG
    CCTGTGCTCT TTGCAGTTGG TGGTCTTCTG CTGCTCTCCA ATGCCTCCTG TGTTGGGGGT
    CTCCTCTGGC GGAGAAGACT GAGGCGCCTT GCTGAGGAGA TCTCAGAGAA GACAGAGGCA
    GGGTCGGAGG ACAGGATCAG GAATGAATAT GAGGAGAGTC AGTGGACTGG GGACCGGGAC
    ACGAGAAGCT CCACGGTTAG CACAGCAGAA GTGGACCCAA ATTACTACTC CATGAGGGAC
    TTCAGCCCCC AGCTTCCCCC AACACTGGAG GAGGTGCTGT ATCACCAAGG TGCTGAAGGC
    GAGGACATGG CCTTCCCCGG ACACCTGCAT GATGAAGTGG AGAGAGCCTA TGGCCCGCCT
    GGGGCCTGGG GACCCCTCTA TGATGAAGTA CGAATGGACC CCTATGACCT TCGCTGGCCT
    GAGGTCCAGT GTGAGGATCC GAGGGGAATC TACGACCAGG TGGCAGCAGA CATGGATGCT
    GTGGAAGCTA GCTCTCTGCC GTTTGAGCTG AGGGGACATC TGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.