Masp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Masp2
Entrez Gene ID	64459
Full Name	mannan-binding lectin serine peptidase 2
Synonyms	MASP-2,MAp19
General protein information	Preferred Names mannan-binding lectin serine peptidase 2 Names mannan-binding lectin serine protease 2 MBL-associated serine protease 2 mannose binding lectin-associated serine protease-2 mannose-binding protein-associated serine protease 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	member of the MBL/MASPs/Map19 complex of the mannan binding lectin pathway of complement activation; catalyzes the cleavage of complement component C4 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017593610.1	XP_017449099.1	mannan-binding lectin serine protease 2 isoform X1
NM_172043.1	NP_742040.1	mannan-binding lectin serine protease 2 precursor
XM_006239401.2	XP_006239463.1	mannan-binding lectin serine protease 2 isoform X3
XM_006239400.3	XP_006239462.1	mannan-binding lectin serine protease 2 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MASP2	XP_544572.4
Bos taurus (cattle)	MASP2	NP_001179001.1
Xenopus tropicalis (tropical clawed frog)	masp2	NP_001011037.1
Mus musculus (house mouse)	Masp2	NP_001003893.1
Rattus norvegicus (Norway rat)	Masp2	NP_742040.1
Homo sapiens (human)	MASP2	NP_006601.2
Pan troglodytes (chimpanzee)	MASP2	XP_001135757.2
Gallus gallus (chicken)	MASP2	NP_998752.1
Danio rerio (zebrafish)	masp2	NP_001116330.1

Gene Ontology
Bibliography

Related articles in PubMed

Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 Provides Insight on Lectin Pathway Activation.
Nan R, Furze CM, Wright DW, Gor J, Wallis R, Perkins SJ
Structure (London, England : 1993)25(2)364-375(2017 02)

Structural basis for activation of the complement system by component C4 cleavage.
Kidmose RT, Laursen NS, Dob? J, Kjaer TR, Sirotkina S, Yatime L, Sottrup-Jensen L, Thiel S, G?l P, Andersen GR
Proceedings of the National Academy of Sciences of the United States of America109(38)15425-30(2012 Sep)

Engineering novel complement activity into a pulmonary surfactant protein.
Venkatraman Girija U, Furze C, Toth J, Schwaeble WJ, Mitchell DA, Keeble AH, Wallis R
The Journal of biological chemistry285(14)10546-52(2010 Apr)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Localization and characterization of the mannose-binding lectin (MBL)-associated-serine protease-2 binding site in rat ficolin-A: equivalent binding sites within the collagenous domains of MBLs and ficolins.
Girija UV, Dodds AW, Roscher S, Reid KB, Wallis R
Journal of immunology (Baltimore, Md. : 1950)179(1)455-62(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Flexibility in MASP-1 and MASP-2 provides insight on lectin pathway activation.
Title: Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 Provides Insight on Lectin Pathway Activation.

A lysine residue (Lys56) within the conserved motif is essential for MASP-2 binding and probably mediates the specificity of the ficolin-MASP interaction.
Title: Localization and characterization of the mannose-binding lectin (MBL)-associated-serine protease-2 binding site in rat ficolin-A: equivalent binding sites within the collagenous domains of MBLs and ficolins.

MASP-2, C4, and C2 and their activation fragments have roles in complement activation via the lectin pathway
Title: Molecular interactions between MASP-2, C4, and C2 and their activation fragments leading to complement activation via the lectin pathway.

Analysis of mutant MBL . MASP-2 complexes assembled from recombinant components shows that reduced complement activation by mutant MBLs is caused by failure to activate MASP-2 efficiently on binding to a mannan-coated surface.
Title: Decoupling of carbohydrate binding and MASP-2 autoactivation in variant mannose-binding lectins associated with immunodeficiency.

Highly conserved intron sequences. Restricted expression contrasts with the ubiquitous expression of neighboring genes.
Title: Organization of the MASP2 locus and its expression profile in mouse and rat.

The following Masp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Masp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05961	NM_172043.1 Latest version!	Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa28904	XM_006239400.3 Latest version!	Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa28905	XM_006239401.2 Latest version!	Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa61373	XM_017593610.1 Latest version!	Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Masp2 ( NM_172043.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742040.1 Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), mRNA.

Price & Availability↑

CloneID	ORa05961
Clone ID Related Accession (Same CDS sequence)	NM_172043.1
Accession Version	NM_172043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	mannan-binding lectin serine protease 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CO557085.1 and AJ277747.1. On Oct 24, 2005 this sequence version replaced XM_342977.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC128700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCTAC TGATCGTCCT GGGTCTGCTT TGGAGTTTGG TGGCCACACT TTTGGGCTCC 
AAGTGGCCTG AGCCTGTATT CGGGCGCCTG GTGTCCCCTG GCTTCCCAGA GAAGTATGGC 
AACCATCAGG ATCGATCCTG GACGCTGACT GCACCCCCTG GCTTCCGCCT GCGCCTCTAC 
TTCACCCACT TCAACCTGGA ACTCTCTTAC CGCTGCGAGT ATGACTTTGT CAAGTTGACC 
TCAGGGACCA AGGTGCTAGC CACGCTGTGT GGGCAGGAGA GTACAGATAC TGAGCGGGCA 
CCTGGCAATG ACACCTTCTA CTCACTGGGT CCCAGCCTAA AGGTCACCTT CCACTCCGAC 
TACTCCAATG AGAAGCCATT CACAGGATTT GAGGCCTTCT ATGCAGCGGA GGATGTGGAT 
GAATGCAGAA CATCCCTGGG AGACTCAGTC CCTTGTGACC ATTATTGCCA CAACTACCTG 
GGCGGCTACT ACTGCTCCTG CCGAGTGGGC TACATTCTGC ACCAGAACAA GCATACCTGC 
TCAGCCCTTT GTTCAGGCCA GGTGTTCACT GGGAGGTCTG GCTTTCTCAG TAGCCCTGAG 
TACCCACAGC CATACCCCAA ACTCTCCAGC TGCGCCTACA ACATCCGCCT GGAGGAAGGC 
TTCAGTATCA CCCTGGACTT CGTGGAGTCC TTTGATGTGG AGATGCACCC TGAAGCCCAG 
TGCCCCTACG ACTCCCTCAA GATTCAAACA GACAAGAGGG AATACGGCCC GTTTTGTGGG 
AAGACGCTGC CCCCCAGGAT TGAAACTGAC AGCAACAAGG TGACCATTAC CTTTACCACC 
GACGAGTCAG GGAACCACAC AGGCTGGAAG ATACACTACA CAAGCACAGC ACAGCCCTGC 
CCTGATCCAA CGGCGCCACC TAATGGTCAC ATTTCACCTG TGCAAGCCAC GTATGTCCTG 
AAGGACAGCT TTTCTGTCTT CTGCAAGACT GGCTTCGAGC TTCTGCAAGG TTCTGTCCCC 
CTGAAGTCAT TCACTGCTGT CTGTCAGAAA GATGGATCTT GGGACCGGCC GATACCAGAG 
TGCAGCATTA TTGACTGTGG CCCTCCCGAT GACCTACCCA ATGGCCACGT GGACTATATC 
ACAGGCCCTG AAGTGACCAC CTACAAAGCT GTGATTCAGT ACAGCTGTGA AGAGACTTTC 
TACACAATGA GCAGCAATGG TAAATATGTG TGTGAGGCTG ATGGATTCTG GACGAGCTCC 
AAAGGAGAAA AATCCCTCCC GGTTTGCAAG CCTGTCTGTG GACTGTCCAC ACACACTTCA 
GGAGGCCGTA TAATTGGAGG ACAGCCTGCA AAGCCTGGTG ACTTTCCTTG GCAAGTCTTG 
TTACTGGGTG AAACTACAGC AGCAGGTGCT CTTATACATG ACGACTGGGT CCTAACAGCG 
GCTCATGCTG TATATGGGAA AACAGAGGCG ATGTCCTCCC TGGACATCCG CATGGGCATC 
CTCAAAAGGC TCTCCCCTCA TTACACTCAA GCCTGGCCAG AGGCTGTCTT TATCCATGAA 
GGCTACACTC ACGGAGCTGG TTTTGACAAT GATATAGCAC TGATTAAACT CAAGAACAAA 
GTCACAATCA ACAGAAACAT CATGCCGATT TGTCTACCAA GAAAAGAAGC TGCATCCTTA 
ATGAAAACAG ACTTCGTTGG AACTGTGGCT GGCTGGGGGT TAACCCAGAA GGGGTTTCTT 
GCTAGAAACC TAATGTTTGT GGACATACCA ATTGTTGACC ACCAAAAATG TGCTACTGCG 
TATACAAAGC AGCCCTACCC AGGAGCAAAA GTGACTGTTA ACATGCTCTG TGCTGGCCTA 
GACGCCGGTG GCAAGGACAG CTGCAGAGGT GACAGCGGAG GGGCATTAGT GTTTCTAGAC 
AATGAAACAC AGAGATGGTT TGTGGGAGGA ATAGTTTCCT GGGGTTCTAT TAACTGTGGG 
GGGTCAGAAC AGTATGGGGT CTACACGAAA GTCACGAACT ATATTCCCTG GATTGAGAAC 
ATAATAAATA ATTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742040.1
CDS	38..2095
Translation	MRLLIVLGLLWSLVATLLGSKWPEPVFGRLVSPGFPEKYGNHQDRSWTLTAPPGFRLRLYFTHFNLELSYRCEYDFVKLTSGTKVLATLCGQESTDTERAPGNDTFYSLGPSLKVTFHSDYSNEKPFTGFEAFYAAEDVDECRTSLGDSVPCDHYCHNYLGGYYCSCRVGYILHQNKHTCSALCSGQVFTGRSGFLSSPEYPQPYPKLSSCAYNIRLEEGFSITLDFVESFDVEMHPEAQCPYDSLKIQTDKREYGPFCGKTLPPRIETDSNKVTITFTTDESGNHTGWKIHYTSTAQPCPDPTAPPNGHISPVQATYVLKDSFSVFCKTGFELLQGSVPLKSFTAVCQKDGSWDRPIPECSIIDCGPPDDLPNGHVDYITGPEVTTYKAVIQYSCEETFYTMSSNGKYVCEADGFWTSSKGEKSLPVCKPVCGLSTHTSGGRIIGGQPAKPGDFPWQVLLLGETTAAGALIHDDWVLTAAHAVYGKTEAMSSLDIRMGILKRLSPHYTQAWPEAVFIHEGYTHGAGFDNDIALIKLKNKVTINRNIMPICLPRKEAASLMKTDFVGTVAGWGLTQKGFLARNLMFVDIPIVDHQKCATAYTKQPYPGAKVTVNMLCAGLDAGGKDSCRGDSGGALVFLDNETQRWFVGGIVSWGSINCGGSEQYGVYTKVTNYIPWIENIINNF

Target ORF information:

RefSeq Version	NM_172043.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172043.1

ORF Insert Sequence:

ATGAGGCTAC TGATCGTCCT GGGTCTGCTT TGGAGTTTGG TGGCCACACT TTTGGGCTCC 
AAGTGGCCTG AGCCTGTATT CGGGCGCCTG GTGTCCCCTG GCTTCCCAGA GAAGTATGGC 
AACCATCAGG ATCGATCCTG GACGCTGACT GCACCCCCTG GCTTCCGCCT GCGCCTCTAC 
TTCACCCACT TCAACCTGGA ACTCTCTTAC CGCTGCGAGT ATGACTTTGT CAAGTTGACC 
TCAGGGACCA AGGTGCTAGC CACGCTGTGT GGGCAGGAGA GTACAGATAC TGAGCGGGCA 
CCTGGCAATG ACACCTTCTA CTCACTGGGT CCCAGCCTAA AGGTCACCTT CCACTCCGAC 
TACTCCAATG AGAAGCCATT CACAGGATTT GAGGCCTTCT ATGCAGCGGA GGATGTGGAT 
GAATGCAGAA CATCCCTGGG AGACTCAGTC CCTTGTGACC ATTATTGCCA CAACTACCTG 
GGCGGCTACT ACTGCTCCTG CCGAGTGGGC TACATTCTGC ACCAGAACAA GCATACCTGC 
TCAGCCCTTT GTTCAGGCCA GGTGTTCACT GGGAGGTCTG GCTTTCTCAG TAGCCCTGAG 
TACCCACAGC CATACCCCAA ACTCTCCAGC TGCGCCTACA ACATCCGCCT GGAGGAAGGC 
TTCAGTATCA CCCTGGACTT CGTGGAGTCC TTTGATGTGG AGATGCACCC TGAAGCCCAG 
TGCCCCTACG ACTCCCTCAA GATTCAAACA GACAAGAGGG AATACGGCCC GTTTTGTGGG 
AAGACGCTGC CCCCCAGGAT TGAAACTGAC AGCAACAAGG TGACCATTAC CTTTACCACC 
GACGAGTCAG GGAACCACAC AGGCTGGAAG ATACACTACA CAAGCACAGC ACAGCCCTGC 
CCTGATCCAA CGGCGCCACC TAATGGTCAC ATTTCACCTG TGCAAGCCAC GTATGTCCTG 
AAGGACAGCT TTTCTGTCTT CTGCAAGACT GGCTTCGAGC TTCTGCAAGG TTCTGTCCCC 
CTGAAGTCAT TCACTGCTGT CTGTCAGAAA GATGGATCTT GGGACCGGCC GATACCAGAG 
TGCAGCATTA TTGACTGTGG CCCTCCCGAT GACCTACCCA ATGGCCACGT GGACTATATC 
ACAGGCCCTG AAGTGACCAC CTACAAAGCT GTGATTCAGT ACAGCTGTGA AGAGACTTTC 
TACACAATGA GCAGCAATGG TAAATATGTG TGTGAGGCTG ATGGATTCTG GACGAGCTCC 
AAAGGAGAAA AATCCCTCCC GGTTTGCAAG CCTGTCTGTG GACTGTCCAC ACACACTTCA 
GGAGGCCGTA TAATTGGAGG ACAGCCTGCA AAGCCTGGTG ACTTTCCTTG GCAAGTCTTG 
TTACTGGGTG AAACTACAGC AGCAGGTGCT CTTATACATG ACGACTGGGT CCTAACAGCG 
GCTCATGCTG TATATGGGAA AACAGAGGCG ATGTCCTCCC TGGACATCCG CATGGGCATC 
CTCAAAAGGC TCTCCCCTCA TTACACTCAA GCCTGGCCAG AGGCTGTCTT TATCCATGAA 
GGCTACACTC ACGGAGCTGG TTTTGACAAT GATATAGCAC TGATTAAACT CAAGAACAAA 
GTCACAATCA ACAGAAACAT CATGCCGATT TGTCTACCAA GAAAAGAAGC TGCATCCTTA 
ATGAAAACAG ACTTCGTTGG AACTGTGGCT GGCTGGGGGT TAACCCAGAA GGGGTTTCTT 
GCTAGAAACC TAATGTTTGT GGACATACCA ATTGTTGACC ACCAAAAATG TGCTACTGCG 
TATACAAAGC AGCCCTACCC AGGAGCAAAA GTGACTGTTA ACATGCTCTG TGCTGGCCTA 
GACGCCGGTG GCAAGGACAG CTGCAGAGGT GACAGCGGAG GGGCATTAGT GTTTCTAGAC 
AATGAAACAC AGAGATGGTT TGTGGGAGGA ATAGTTTCCT GGGGTTCTAT TAACTGTGGG 
GGGTCAGAAC AGTATGGGGT CTACACGAAA GTCACGAACT ATATTCCCTG GATTGAGAAC 
ATAATAAATA ATTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Masp2 ( XM_006239400.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239462.1 Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa28904
Clone ID Related Accession (Same CDS sequence)	XM_006239400.3
Accession Version	XM_006239400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mannan-binding lectin serine protease 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239400.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTAC TGATCGTCCT GGGTCTGCTT TGGAGTTTGG TGGCCACACT TTTGGGCTCC 
AAGTGGCCTG AGCCTGTATT CGGGCGCCTG GTGTCCCCTG GCTTCCCAGA GAAGTATGGC 
AACCATCAGG ATCGATCCTG GACGCTGACT GCACCCCCTG GCTTCCGCCT GCGCCTCTAC 
TTCACCCACT TCAACCTGGA ACTCTCTTAC CGCTGCGAGT ATGACTTTGT CAAGTTGACC 
TCAGGGACCA AGGTGCTAGC CACGCTGTGT GGGCAGGAGA GTACAGATAC TGAGCGGGCA 
CCTGGCAATG ACACCTTCTA CTCACTGGGT CCCAGCCTAA AGGTCACCTT CCACTCCGAC 
TACTCCAATG AGAAGCCATT CACAGGATTT GAGGCCTTCT ATGCAGCGGA GGATGTGGAT 
GAATGCAGAA CATCCCTGGG AGACTCAGTC CCTTGTGACC ATTATTGCCA CAACTACCTG 
GGCGGCTACT ACTGCTCCTG CCGAGTGGGC TACATTCTGC ACCAGAACAA GCATACCTGC 
TCAGCCCTTT GTTCAGGCCA GGTGTTCACT GGGAGGTCTG GCTTTCTCAG TAGCCCTGAG 
TACCCACAGC CATACCCCAA ACTCTCCAGC TGCGCCTACA ACATCCGCCT GGAGGAAGGC 
TTCAGTATCA CCCTGGACTT CGTGGAGTCC TTTGATGTGG AGATGCACCC TGAAGCCCAG 
TGCCCCTACG ACTCCCTCAA GATTCAAACA GACAAGAGGG AATACGGCCC GTTTTGTGGG 
AAGACGCTGC CCCCCAGGAT TGAAACTGAC AGCAACAAGG TGACCATTAC CTTTACCACC 
GACGAGTCAG GGAACCACAC AGGCTGGAAG ATACACTACA CAAGCACAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239462.1
CDS	94..987
Translation	MRLLIVLGLLWSLVATLLGSKWPEPVFGRLVSPGFPEKYGNHQDRSWTLTAPPGFRLRLYFTHFNLELSYRCEYDFVKLTSGTKVLATLCGQESTDTERAPGNDTFYSLGPSLKVTFHSDYSNEKPFTGFEAFYAAEDVDECRTSLGDSVPCDHYCHNYLGGYYCSCRVGYILHQNKHTCSALCSGQVFTGRSGFLSSPEYPQPYPKLSSCAYNIRLEEGFSITLDFVESFDVEMHPEAQCPYDSLKIQTDKREYGPFCGKTLPPRIETDSNKVTITFTTDESGNHTGWKIHYTSTD

Target ORF information:

RefSeq Version	XM_006239400.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239400.3

ORF Insert Sequence:

ATGAGGCTAC TGATCGTCCT GGGTCTGCTT TGGAGTTTGG TGGCCACACT TTTGGGCTCC 
AAGTGGCCTG AGCCTGTATT CGGGCGCCTG GTGTCCCCTG GCTTCCCAGA GAAGTATGGC 
AACCATCAGG ATCGATCCTG GACGCTGACT GCACCCCCTG GCTTCCGCCT GCGCCTCTAC 
TTCACCCACT TCAACCTGGA ACTCTCTTAC CGCTGCGAGT ATGACTTTGT CAAGTTGACC 
TCAGGGACCA AGGTGCTAGC CACGCTGTGT GGGCAGGAGA GTACAGATAC TGAGCGGGCA 
CCTGGCAATG ACACCTTCTA CTCACTGGGT CCCAGCCTAA AGGTCACCTT CCACTCCGAC 
TACTCCAATG AGAAGCCATT CACAGGATTT GAGGCCTTCT ATGCAGCGGA GGATGTGGAT 
GAATGCAGAA CATCCCTGGG AGACTCAGTC CCTTGTGACC ATTATTGCCA CAACTACCTG 
GGCGGCTACT ACTGCTCCTG CCGAGTGGGC TACATTCTGC ACCAGAACAA GCATACCTGC 
TCAGCCCTTT GTTCAGGCCA GGTGTTCACT GGGAGGTCTG GCTTTCTCAG TAGCCCTGAG 
TACCCACAGC CATACCCCAA ACTCTCCAGC TGCGCCTACA ACATCCGCCT GGAGGAAGGC 
TTCAGTATCA CCCTGGACTT CGTGGAGTCC TTTGATGTGG AGATGCACCC TGAAGCCCAG 
TGCCCCTACG ACTCCCTCAA GATTCAAACA GACAAGAGGG AATACGGCCC GTTTTGTGGG 
AAGACGCTGC CCCCCAGGAT TGAAACTGAC AGCAACAAGG TGACCATTAC CTTTACCACC 
GACGAGTCAG GGAACCACAC AGGCTGGAAG ATACACTACA CAAGCACAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Masp2 ( XM_006239401.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239463.1 Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa28905
Clone ID Related Accession (Same CDS sequence)	XM_006239401.2
Accession Version	XM_006239401.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mannan-binding lectin serine protease 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006239401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTAC TGATCGTCCT GGGTCTGCTT TGGAGTTTGG TGGCCACACT TTTGGGCTCC 
AAGTGGCCTG AGCCTGTATT CGGGCGCCTG GTGTCCCCTG GCTTCCCAGA GAAGTATGGC 
AACCATCAGG ATCGATCCTG GACGCTGACT GCACCCCCTG GCTTCCGCCT GCGCCTCTAC 
TTCACCCACT TCAACCTGGA ACTCTCTTAC CGCTGCGAGT ATGACTTTGT CAAGTTGACC 
TCAGGGACCA AGGTGCTAGC CACGCTGTGT GGGCAGGAGA GTACAGATAC TGAGCGGGCA 
CCTGGCAATG ACACCTTCTA CTCACTGGGT CCCAGCCTAA AGGTCACCTT CCACTCCGAC 
TACTCCAATG AGAAGCCATT CACAGGATTT GAGGCCTTCT ATGCAGCGGA GGATGTGGAT 
GAATGCAGAA CATCCCTGGG AGACTCAGTC CCTTGTGACC ATTATTGCCA CAACTACCTG 
GGCGGCTACT ACTGCTCCTG CCGAGTGGGC TACATTCTGC ACCAGAACAA GCATACCTGC 
TCAGAGCAGA GCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239463.1
CDS	93..650
Translation	MRLLIVLGLLWSLVATLLGSKWPEPVFGRLVSPGFPEKYGNHQDRSWTLTAPPGFRLRLYFTHFNLELSYRCEYDFVKLTSGTKVLATLCGQESTDTERAPGNDTFYSLGPSLKVTFHSDYSNEKPFTGFEAFYAAEDVDECRTSLGDSVPCDHYCHNYLGGYYCSCRVGYILHQNKHTCSEQSL

Target ORF information:

RefSeq Version	XM_006239401.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239401.2

ORF Insert Sequence:

ATGAGGCTAC TGATCGTCCT GGGTCTGCTT TGGAGTTTGG TGGCCACACT TTTGGGCTCC 
AAGTGGCCTG AGCCTGTATT CGGGCGCCTG GTGTCCCCTG GCTTCCCAGA GAAGTATGGC 
AACCATCAGG ATCGATCCTG GACGCTGACT GCACCCCCTG GCTTCCGCCT GCGCCTCTAC 
TTCACCCACT TCAACCTGGA ACTCTCTTAC CGCTGCGAGT ATGACTTTGT CAAGTTGACC 
TCAGGGACCA AGGTGCTAGC CACGCTGTGT GGGCAGGAGA GTACAGATAC TGAGCGGGCA 
CCTGGCAATG ACACCTTCTA CTCACTGGGT CCCAGCCTAA AGGTCACCTT CCACTCCGAC 
TACTCCAATG AGAAGCCATT CACAGGATTT GAGGCCTTCT ATGCAGCGGA GGATGTGGAT 
GAATGCAGAA CATCCCTGGG AGACTCAGTC CCTTGTGACC ATTATTGCCA CAACTACCTG 
GGCGGCTACT ACTGCTCCTG CCGAGTGGGC TACATTCTGC ACCAGAACAA GCATACCTGC 
TCAGAGCAGA GCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Masp2 ( XM_017593610.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449099.1 Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), transcript variant X1, mRNA.

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CloneID	ORa61373
Clone ID Related Accession (Same CDS sequence)	XM_017593610.1
Accession Version	XM_017593610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mannan-binding lectin serine protease 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTAC TGATCGTCCT GGGTCTGCTT TGGAGTTTGG TGGCCACACT TTTGGGCTCC 
AAGTGGCCTG AGCCTGTATT CGGGCGCCTG GTGTCCCCTG GCTTCCCAGA GAAGTATGGC 
AACCATCAGG ATCGATCCTG GACGCTGACT GCACCCCCTG GCTTCCGCCT GCGCCTCTAC 
TTCACCCACT TCAACCTGGA ACTCTCTTAC CGCTGCGAGT ATGACTTTGT CAAGTTGACC 
TCAGGGACCA AGGTGCTAGC CACGCTGTGT GGGCAGGAGA GTACAGATAC TGAGCGGGCA 
CCTGGCAATG ACACCTTCTA CTCACTGGGT CCCAGCCTAA AGGTCACCTT CCACTCCGAC 
TACTCCAATG AGAAGCCATT CACAGGATTT GAGGCCTTCT ATGCAGCGGA GGATGTGGAT 
GAATGCAGAA CATCCCTGGG AGACTCAGTC CCTTGTGACC ATTATTGCCA CAACTACCTG 
GGCGGCTACT ACTGCTCCTG CCGAGTGGGC TACATTCTGC ACCAGAACAA GCATACCTGC 
TCAGCCCTTT GTTCAGGCCA GGTGTTCACT GGGAGGTCTG GCTTTCTCAG TAGCCCTGAG 
TACCCACAGC CATACCCCAA ACTCTCCAGC TGCGCCTACA ACATCCGCCT GGAGGAAGGC 
TTCAGTATCA CCCTGGACTT CGTGGAGTCC TTTGATGTGG AGATGCACCC TGAAGCCCAG 
TGCCCCTACG ACTCCCTCAA GATTCAAACA GACAAGAGGG AATACGGCCC GTTTTGTGGG 
AAGACGCTGC CCCCCAGGAT TGAAACTGAC AGCAACAAGG TGACCATTAC CTTTACCACC 
GACGAGTCAG GGAACCACAC AGGCTGGAAG ATACACTACA CAAGCACAGC ACAGCCCTGC 
CCTGATCCAA CGGCGCCACC TAATGGTCAC ATTTCACCTG TGCAAGCCAC GTATGTCCTG 
AAGGACAGCT TTTCTGTCTT CTGCAAGACT GGCTTCGAGC TTCTGCAAGG TTCTGTCCCC 
CTGAAGTCAT TCACTGCTGT CTGTCAGAAA GATGGATCTT GGGACCGGCC GATACCAGAG 
TGCAGCATTA TTGACTGTGG CCCTCCCGAT GACCTACCCA ATGGCCACGT GGACTATATC 
ACAGGCCCTG AAGTGACCAC CTACAAAGCT GTGATTCAGT ACAGCTGTGA AGAGACTTTC 
TACACAATGA GCAGCAATGG TAAATATGTG TGTGAGGCTG ATGGATTCTG GACGAGCTCC 
AAAGGAGAAA AATCCCTCCC GGTTTGCAAG CCTGGTAATG GACGCATTTA TAAAAGAGAT 
TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449099.1
CDS	94..1419
Translation	MRLLIVLGLLWSLVATLLGSKWPEPVFGRLVSPGFPEKYGNHQDRSWTLTAPPGFRLRLYFTHFNLELSYRCEYDFVKLTSGTKVLATLCGQESTDTERAPGNDTFYSLGPSLKVTFHSDYSNEKPFTGFEAFYAAEDVDECRTSLGDSVPCDHYCHNYLGGYYCSCRVGYILHQNKHTCSALCSGQVFTGRSGFLSSPEYPQPYPKLSSCAYNIRLEEGFSITLDFVESFDVEMHPEAQCPYDSLKIQTDKREYGPFCGKTLPPRIETDSNKVTITFTTDESGNHTGWKIHYTSTAQPCPDPTAPPNGHISPVQATYVLKDSFSVFCKTGFELLQGSVPLKSFTAVCQKDGSWDRPIPECSIIDCGPPDDLPNGHVDYITGPEVTTYKAVIQYSCEETFYTMSSNGKYVCEADGFWTSSKGEKSLPVCKPGNGRIYKRDS

Target ORF information:

RefSeq Version	XM_017593610.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannan-binding lectin serine peptidase 2 (Masp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593610.1

ORF Insert Sequence:

ATGAGGCTAC TGATCGTCCT GGGTCTGCTT TGGAGTTTGG TGGCCACACT TTTGGGCTCC 
AAGTGGCCTG AGCCTGTATT CGGGCGCCTG GTGTCCCCTG GCTTCCCAGA GAAGTATGGC 
AACCATCAGG ATCGATCCTG GACGCTGACT GCACCCCCTG GCTTCCGCCT GCGCCTCTAC 
TTCACCCACT TCAACCTGGA ACTCTCTTAC CGCTGCGAGT ATGACTTTGT CAAGTTGACC 
TCAGGGACCA AGGTGCTAGC CACGCTGTGT GGGCAGGAGA GTACAGATAC TGAGCGGGCA 
CCTGGCAATG ACACCTTCTA CTCACTGGGT CCCAGCCTAA AGGTCACCTT CCACTCCGAC 
TACTCCAATG AGAAGCCATT CACAGGATTT GAGGCCTTCT ATGCAGCGGA GGATGTGGAT 
GAATGCAGAA CATCCCTGGG AGACTCAGTC CCTTGTGACC ATTATTGCCA CAACTACCTG 
GGCGGCTACT ACTGCTCCTG CCGAGTGGGC TACATTCTGC ACCAGAACAA GCATACCTGC 
TCAGCCCTTT GTTCAGGCCA GGTGTTCACT GGGAGGTCTG GCTTTCTCAG TAGCCCTGAG 
TACCCACAGC CATACCCCAA ACTCTCCAGC TGCGCCTACA ACATCCGCCT GGAGGAAGGC 
TTCAGTATCA CCCTGGACTT CGTGGAGTCC TTTGATGTGG AGATGCACCC TGAAGCCCAG 
TGCCCCTACG ACTCCCTCAA GATTCAAACA GACAAGAGGG AATACGGCCC GTTTTGTGGG 
AAGACGCTGC CCCCCAGGAT TGAAACTGAC AGCAACAAGG TGACCATTAC CTTTACCACC 
GACGAGTCAG GGAACCACAC AGGCTGGAAG ATACACTACA CAAGCACAGC ACAGCCCTGC 
CCTGATCCAA CGGCGCCACC TAATGGTCAC ATTTCACCTG TGCAAGCCAC GTATGTCCTG 
AAGGACAGCT TTTCTGTCTT CTGCAAGACT GGCTTCGAGC TTCTGCAAGG TTCTGTCCCC 
CTGAAGTCAT TCACTGCTGT CTGTCAGAAA GATGGATCTT GGGACCGGCC GATACCAGAG 
TGCAGCATTA TTGACTGTGG CCCTCCCGAT GACCTACCCA ATGGCCACGT GGACTATATC 
ACAGGCCCTG AAGTGACCAC CTACAAAGCT GTGATTCAGT ACAGCTGTGA AGAGACTTTC 
TACACAATGA GCAGCAATGG TAAATATGTG TGTGAGGCTG ATGGATTCTG GACGAGCTCC 
AAAGGAGAAA AATCCCTCCC GGTTTGCAAG CCTGGTAATG GACGCATTTA TAAAAGAGAT 
TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 Provides Insight on Lectin Pathway Activation.
Structure (London, England : 1993)25(2)364-375(2017 02)
Nan R,Furze CM,Wright DW,Gor J,Wallis R,Perkins SJ

Structural basis for activation of the complement system by component C4 cleavage.
Proceedings of the National Academy of Sciences of the United States of America109(38)15425-30(2012 Sep)
Kidmose RT,Laursen NS,Dob? J,Kjaer TR,Sirotkina S,Yatime L,Sottrup-Jensen L,Thiel S,G?l P,Andersen GR

Engineering novel complement activity into a pulmonary surfactant protein.
The Journal of biological chemistry285(14)10546-52(2010 Apr)
Venkatraman Girija U,Furze C,Toth J,Schwaeble WJ,Mitchell DA,Keeble AH,Wallis R

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Localization and characterization of the mannose-binding lectin (MBL)-associated-serine protease-2 binding site in rat ficolin-A: equivalent binding sites within the collagenous domains of MBLs and ficolins.
Journal of immunology (Baltimore, Md. : 1950)179(1)455-62(2007 Jul)
Girija UV,Dodds AW,Roscher S,Reid KB,Wallis R

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Masp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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