Isl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Isl1
Entrez Gene ID	64444
Full Name	ISL LIM homeobox 1
Synonyms	Isl-1,isl-1=homeobox
General protein information	Preferred Names ISL LIM homeobox 1 Names insulin gene enhancer protein ISL-1 ISL1 transcription factor LIM/homeodomain (islet-1) ISL1 transcription factor, LIM/homeodomain 1 isl-1 homeobox islet-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q14
Summary	transcription factor; may regulate insulin gene expression or islet cell development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017339.3	NP_059035.3	insulin gene enhancer protein ISL-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ISL1	NP_002193.2
Pan troglodytes (chimpanzee)	ISL1	XP_001150633.2
Canis lupus familiaris (dog)	LOC612954	XP_853721.2
Rattus norvegicus (Norway rat)	Isl1	NP_059035.3
Gallus gallus (chicken)	ISL1	NP_990745.1
Caenorhabditis elegans (roundworm)	lim-7	NP_491668.1
Bos taurus (cattle)	ISL1	NP_001092600.1
Drosophila melanogaster (fruit fly)	tup	NP_476774.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010209	XP_319393.4
Mus musculus (house mouse)	Isl1	NP_067434.3
Danio rerio (zebrafish)	isl1	NP_571037.1

Gene Ontology
Bibliography

Related articles in PubMed

An in vitro model for the assessment of stem cell fate following implantation within the infarct microenvironment identifies ISL-1 expression as the strongest predictor of c-Kit(+) cardiac progenitor cells' therapeutic potential.
Sullivan KE, Burns LJ, Black LD 3rd
Journal of molecular and cellular cardiology8891-100(2015 Nov)

Two transcription factors, Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate.
Wu F, Kaczynski TJ, Sethuramanujam S, Li R, Jain V, Slaughter M, Mu X
Proceedings of the National Academy of Sciences of the United States of America112(13)E1559-68(2015 Mar)

Nf2-Yap signaling controls the expansion of DRG progenitors and glia during DRG development.
Serinagaoglu Y, Par? J, Giovannini M, Cao X
Developmental biology398(1)97-109(2015 Feb)

Prdm1 functions in the mesoderm of the second heart field, where it interacts genetically with Tbx1, during outflow tract morphogenesis in the mouse embryo.
Vincent SD, Mayeuf-Louchart A, Watanabe Y, Brzezinski JA 4th, Miyagawa-Tomita S, Kelly RG, Buckingham M
Human molecular genetics23(19)5087-101(2014 Oct)

Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells.
Li R, Wu F, Ruonala R, Sapkota D, Hu Z, Mu X
PloS one9(3)e92105(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An in vitro model for the assessment of stem cell fate following implantation within the infarct microenvironment identifies ISL-1 expression as the strongest predictor of c-Kit(+) cardiac progenitor cells' therapeutic potential.
Title: An in vitro model for the assessment of stem cell fate following implantation within the infarct microenvironment identifies ISL-1 expression as the strongest predictor of c-Kit(+) cardiac progenitor cells' therapeutic potential.

Overexpression of LMX1B was associated with reduced cell apoptosis, whilst downregulation of LMX1B was associated with increased cell apoptosis in H-R RTEC.
Title: LIM homeobox transcription factor 1B is associated with pro-fibrotic components and apoptosis in hypoxia/reoxygenation renal tubular epithelial cells.

Isl1 is a key regulatory molecule in adult cardiac epithelial-to-mesenchymal transition.
Title: Islet-1 is a dual regulator of fibrogenic epithelial-to-mesenchymal transition in epicardial mesothelial cells.

Isl1-Lhx3, a LIM-complex mimicking fusion, induces a signature of motor neuron transcriptome & suppresses interneuron differentiation programs. An equimolar ratio of Isl1 & Lhx3 & the LIM domain of Lhx3 are crucial for this.
Title: Fusion protein Isl1-Lhx3 specifies motor neuron fate by inducing motor neuron genes and concomitantly suppressing the interneuron programs.

Subventricular zone neurons can be redirected from their normal migration route and directed towards other brain regions when they are infected with retroviruses encoding the developmentally important transcription factors islet-1 and neurogenin-2.
Title: Reprogramming of neonatal SVZ progenitors by islet-1 and neurogenin-2.

The following Isl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Isl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03689	NM_017339.3 Latest version!	Rattus norvegicus ISL LIM homeobox 1 (Isl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Isl1 ( NM_017339.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059035.3 Rattus norvegicus ISL LIM homeobox 1 (Isl1), mRNA.

Price & Availability↑

CloneID	ORa03689
Clone ID Related Accession (Same CDS sequence)	NM_017339.3
Accession Version	NM_017339.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	insulin gene enhancer protein ISL-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S69329.1. On Apr 28, 2006 this sequence version replaced NM_017339.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S69329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGACA TGGGCGATCC ACCAAAAAAA AAACGTCTGA TTTCCCTATG TGTTGGTTGC 
GGTAATCAAA TTCACGATCA GTATATTCTG AGGGTTTCTC CGGATTTGGA ATGGCATGCG 
GCATGTTTGA AATGTGCGGA GTGTAATCAG TATTTGGACG AAAGCTGTAC CTGCTTTGTT 
AGGGACGGGA AAACCTACTG TAAAAGAGAT TATATCAGGT TGTACGGGAT CAAATGCGCC 
AAGTGCAGCA TAGGCTTCAG CAAGAACGAC TTCGTGATGC GCGCCCGCTC TAAGGTGTAC 
CACATCGAAT GTTTCCGCTG TGTAGCATGC AGCCGACAGC TCATCCCGGG AGACGAATTC 
GCGCTGCGGG AGGATGGGCT TTTCTGCCGC GCGGACCACG ATGTAGTGGA GAGGGCCAGC 
CTAGGAGCTG GAGACCCTCT CAGTCCCTTG CATCCAGCGC GGCCTCTGCA AATGGCAGCC 
GAGCCCATCT CCGCTAGGCA GCCAGCTCTG CGGCCGCACG TCCACAAACA GCCCGAGAAG 
ACCACCCGAG TGCGGACTGT GCTCAACGAA AAGCAGCTGC ACACCTTGCG GACCTGCTAC 
GCAGCCAACC CTCGGCCAGA TGCGCTCATG AAGGAGCAAC TAGTGGAGAT GACCGGCCTC 
AGTCCCCGAG TCATCCGGGT CTGGTTTCAA AACAAGAGGT GCAAGGACAA GAAACGCAGC 
ATCATGATGA AGCAGCTCCA GCAGCAGCAA CCCAACGACA AAACTAATAT CCAGGGGATG 
ACAGGAACTC CCATGGTGGC TGCTAGTCCG GAGAGACATG ATGGTGGTTT ACAGGCTAAC 
CCAGTTGAGG TGCAAAGTTA CCAGCCGCCC TGGAAAGTAC TGAGTGACTT CGCCTTGCAA 
AGTGACATAG ATCAGCCTGC TTTTCAGCAA CTGGTCAATT TTTCAGAAGG AGGACCAGGC 
TCTAATTCCA CTGGCAGTGA AGTAGCATCG ATGTCCTCTC AGCTCCCAGA TACACCCAAC 
AGCATGGTAG CCAGTCCTAT AGAGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059035.3
CDS	6..1055
Translation	MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKCAECNQYLDESCTCFVRDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPNSMVASPIEA

Target ORF information:

RefSeq Version	NM_017339.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ISL LIM homeobox 1 (Isl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017339.3

ORF Insert Sequence:

ATGGGAGACA TGGGCGATCC ACCAAAAAAA AAACGTCTGA TTTCCCTATG TGTTGGTTGC 
GGTAATCAAA TTCACGATCA GTATATTCTG AGGGTTTCTC CGGATTTGGA ATGGCATGCG 
GCATGTTTGA AATGTGCGGA GTGTAATCAG TATTTGGACG AAAGCTGTAC CTGCTTTGTT 
AGGGACGGGA AAACCTACTG TAAAAGAGAT TATATCAGGT TGTACGGGAT CAAATGCGCC 
AAGTGCAGCA TAGGCTTCAG CAAGAACGAC TTCGTGATGC GCGCCCGCTC TAAGGTGTAC 
CACATCGAAT GTTTCCGCTG TGTAGCATGC AGCCGACAGC TCATCCCGGG AGACGAATTC 
GCGCTGCGGG AGGATGGGCT TTTCTGCCGC GCGGACCACG ATGTAGTGGA GAGGGCCAGC 
CTAGGAGCTG GAGACCCTCT CAGTCCCTTG CATCCAGCGC GGCCTCTGCA AATGGCAGCC 
GAGCCCATCT CCGCTAGGCA GCCAGCTCTG CGGCCGCACG TCCACAAACA GCCCGAGAAG 
ACCACCCGAG TGCGGACTGT GCTCAACGAA AAGCAGCTGC ACACCTTGCG GACCTGCTAC 
GCAGCCAACC CTCGGCCAGA TGCGCTCATG AAGGAGCAAC TAGTGGAGAT GACCGGCCTC 
AGTCCCCGAG TCATCCGGGT CTGGTTTCAA AACAAGAGGT GCAAGGACAA GAAACGCAGC 
ATCATGATGA AGCAGCTCCA GCAGCAGCAA CCCAACGACA AAACTAATAT CCAGGGGATG 
ACAGGAACTC CCATGGTGGC TGCTAGTCCG GAGAGACATG ATGGTGGTTT ACAGGCTAAC 
CCAGTTGAGG TGCAAAGTTA CCAGCCGCCC TGGAAAGTAC TGAGTGACTT CGCCTTGCAA 
AGTGACATAG ATCAGCCTGC TTTTCAGCAA CTGGTCAATT TTTCAGAAGG AGGACCAGGC 
TCTAATTCCA CTGGCAGTGA AGTAGCATCG ATGTCCTCTC AGCTCCCAGA TACACCCAAC 
AGCATGGTAG CCAGTCCTAT AGAGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An in vitro model for the assessment of stem cell fate following implantation within the infarct microenvironment identifies ISL-1 expression as the strongest predictor of c-Kit(+) cardiac progenitor cells' therapeutic potential.
Journal of molecular and cellular cardiology8891-100(2015 Nov)
Sullivan KE,Burns LJ,Black LD 3rd

Two transcription factors, Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate.
Proceedings of the National Academy of Sciences of the United States of America112(13)E1559-68(2015 Mar)
Wu F,Kaczynski TJ,Sethuramanujam S,Li R,Jain V,Slaughter M,Mu X

Nf2-Yap signaling controls the expansion of DRG progenitors and glia during DRG development.
Developmental biology398(1)97-109(2015 Feb)
Serinagaoglu Y,Par? J,Giovannini M,Cao X

Prdm1 functions in the mesoderm of the second heart field, where it interacts genetically with Tbx1, during outflow tract morphogenesis in the mouse embryo.
Human molecular genetics23(19)5087-101(2014 Oct)
Vincent SD,Mayeuf-Louchart A,Watanabe Y,Brzezinski JA 4th,Miyagawa-Tomita S,Kelly RG,Buckingham M

Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells.
PloS one9(3)e92105(2014)
Li R,Wu F,Ruonala R,Sapkota D,Hu Z,Mu X

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Isl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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