A4galt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	A4galt
Entrez Gene ID	63888
Full Name	alpha 1,4-galactosyltransferase
Synonyms	Gb3
General protein information	Preferred Names alpha 1,4-galactosyltransferase Names lactosylceramide 4-alpha-galactosyltransferase Gb3 synthase UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase alpha-1,4-N-acetylglucosaminyltransferase alpha4Gal-T1 globotriaosylceramide synthase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	transfers a UDP-sugar in an alpha1, 4-linkage to a beta-linked Gal found in mucin [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017595078.1	XP_017450567.1	lactosylceramide 4-alpha-galactosyltransferase isoform X1
XM_017595079.1	XP_017450568.1	lactosylceramide 4-alpha-galactosyltransferase isoform X2
NM_022240.2	NP_071576.1	lactosylceramide 4-alpha-galactosyltransferase
XM_006242118.3	XP_006242180.1	lactosylceramide 4-alpha-galactosyltransferase isoform X2
XM_017595081.1	XP_017450570.1	lactosylceramide 4-alpha-galactosyltransferase isoform X2
XM_017595080.1	XP_017450569.1	lactosylceramide 4-alpha-galactosyltransferase isoform X2
XM_006242117.3	XP_006242179.1	lactosylceramide 4-alpha-galactosyltransferase isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	A4GALT	NP_059132.1
Bos taurus (cattle)	A4GALT	XP_005207512.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008259	XP_001237894.2
Pan troglodytes (chimpanzee)	A4GALT	NP_001009123.1
Drosophila melanogaster (fruit fly)	alpha4GT2	NP_651434.3
Rattus norvegicus (Norway rat)	A4galt	NP_071576.1
Mus musculus (house mouse)	A4galt	NP_001004150.1
	A4GALT	XP_002798424.1
Canis lupus familiaris (dog)	A4GALT	XP_005625846.1
Drosophila melanogaster (fruit fly)	alpha4GT1	NP_608737.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008258	XP_317211.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008260	XP_555205.1
Xenopus tropicalis (tropical clawed frog)	a4galt	XP_002942110.1

Gene Ontology
Bibliography

Related articles in PubMed

Globosides but not isoglobosides can impact the development of invariant NKT cells and their interaction with dendritic cells.
Porubsky S, Speak AO, Salio M, Jennemann R, Bonrouhi M, Zafarulla R, Singh Y, Dyson J, Luckow B, Lehuen A, Malle E, M?thing J, Platt FM, Cerundolo V, Gr?ne HJ
Journal of immunology (Baltimore, Md. : 1950)189(6)3007-17(2012 Sep)

Targeted disruption of Gb3/CD77 synthase gene resulted in the complete deletion of globo-series glycosphingolipids and loss of sensitivity to verotoxins.
Okuda T, Tokuda N, Numata S, Ito M, Ohta M, Kawamura K, Wiels J, Urano T, Tajima O, Furukawa K, Furukawa K
The Journal of biological chemistry281(15)10230-5(2006 Apr)

Cloning of Gb3 synthase, the key enzyme in globo-series glycosphingolipid synthesis, predicts a family of alpha 1, 4-glycosyltransferases conserved in plants, insects, and mammals.
Keusch JJ, Manzella SM, Nyame KA, Cummings RD, Baenziger JU
The Journal of biological chemistry275(33)25315-21(2000 Aug)

Cloning and expression of the histo-blood group Pk UDP-galactose: Ga1beta-4G1cbeta1-cer alpha1, 4-galactosyltransferase. Molecular genetic basis of the p phenotype.
Steffensen R, Carlier K, Wiels J, Levery SB, Stroud M, Cedergren B, Nilsson Sojka B, Bennett EP, Jersild C, Clausen H
The Journal of biological chemistry275(22)16723-9(2000 Jun)

Molecular cloning of globotriaosylceramide/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids.
Kojima Y, Fukumoto S, Furukawa K, Okajima T, Wiels J, Yokoyama K, Suzuki Y, Urano T, Ohta M, Furukawa K
The Journal of biological chemistry275(20)15152-6(2000 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following A4galt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the A4galt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05514	NM_022240.2 Latest version!	Rattus norvegicus alpha 1,4-galactosyltransferase (P blood group) (A4galt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa05514	XM_006242117.3 Latest version!	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa05514	XM_017595079.1 Latest version!	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa05514	XM_006242118.3 Latest version!	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa05514	XM_017595080.1 Latest version!	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa05514	XM_017595081.1 Latest version!	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa62138	XM_017595078.1 Latest version!	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

A4galt ( NM_022240.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071576.1 Rattus norvegicus alpha 1,4-galactosyltransferase (P blood group) (A4galt), mRNA.

Price & Availability↑

CloneID	ORa05514
Clone ID Related Accession (Same CDS sequence)	XM_017595079.1 , NM_022240.2 , XM_006242118.3 , XM_017595081.1 , XM_017595080.1 , XM_006242117.3
Accession Version	NM_022240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	lactosylceramide 4-alpha-galactosyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097323.1. On Jun 19, 2005 this sequence version replaced NM_022240.1. ##Evidence-Data-START## Transcript exon combination :: BC097323.1, AF248544.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071576.1
CDS	30..1112
Translation	MGISRSDLEETMSKPPDCLPRMLRGTPRQRVFTLFIISFKFTFLVSILIYWHTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL

Target ORF information:

RefSeq Version	NM_022240.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha 1,4-galactosyltransferase (P blood group) (A4galt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022240.2

ORF Insert Sequence:

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

A4galt ( XM_017595079.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450568.1 Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa05514
Clone ID Related Accession (Same CDS sequence)	XM_017595079.1 , NM_022240.2 , XM_006242118.3 , XM_017595081.1 , XM_017595080.1 , XM_006242117.3
Accession Version	XM_017595079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lactosylceramide 4-alpha-galactosyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450568.1
CDS	1050..2132
Translation	MGISRSDLEETMSKPPDCLPRMLRGTPRQRVFTLFIISFKFTFLVSILIYWHTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL

Target ORF information:

RefSeq Version	XM_017595079.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595079.1

ORF Insert Sequence:

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

A4galt ( XM_006242118.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242180.1 Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa05514
Clone ID Related Accession (Same CDS sequence)	XM_017595079.1 , NM_022240.2 , XM_006242118.3 , XM_017595081.1 , XM_017595080.1 , XM_006242117.3
Accession Version	XM_006242118.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lactosylceramide 4-alpha-galactosyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242118.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242180.1
CDS	991..2073
Translation	MGISRSDLEETMSKPPDCLPRMLRGTPRQRVFTLFIISFKFTFLVSILIYWHTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL

Target ORF information:

RefSeq Version	XM_006242118.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242118.3

ORF Insert Sequence:

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

A4galt ( XM_017595081.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450570.1 Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa05514
Clone ID Related Accession (Same CDS sequence)	XM_017595079.1 , NM_022240.2 , XM_006242118.3 , XM_017595081.1 , XM_017595080.1 , XM_006242117.3
Accession Version	XM_017595081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lactosylceramide 4-alpha-galactosyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450570.1
CDS	430..1512
Translation	MGISRSDLEETMSKPPDCLPRMLRGTPRQRVFTLFIISFKFTFLVSILIYWHTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL

Target ORF information:

RefSeq Version	XM_017595081.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595081.1

ORF Insert Sequence:

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

A4galt ( XM_017595080.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450569.1 Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa05514
Clone ID Related Accession (Same CDS sequence)	XM_017595079.1 , NM_022240.2 , XM_006242118.3 , XM_017595081.1 , XM_017595080.1 , XM_006242117.3
Accession Version	XM_017595080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lactosylceramide 4-alpha-galactosyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450569.1
CDS	908..1990
Translation	MGISRSDLEETMSKPPDCLPRMLRGTPRQRVFTLFIISFKFTFLVSILIYWHTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL

Target ORF information:

RefSeq Version	XM_017595080.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595080.1

ORF Insert Sequence:

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

A4galt ( XM_006242117.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242179.1 Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa05514
Clone ID Related Accession (Same CDS sequence)	XM_017595079.1 , NM_022240.2 , XM_006242118.3 , XM_017595081.1 , XM_017595080.1 , XM_006242117.3
Accession Version	XM_006242117.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lactosylceramide 4-alpha-galactosyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242117.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242179.1
CDS	573..1655
Translation	MGISRSDLEETMSKPPDCLPRMLRGTPRQRVFTLFIISFKFTFLVSILIYWHTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL

Target ORF information:

RefSeq Version	XM_006242117.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242117.3

ORF Insert Sequence:

ATGGGCATCT CCCGTTCGGA TCTGGAGGAG ACCATGTCCA AGCCCCCCGA CTGCCTGCCT 
CGGATGCTCA GGGGCACGCC CAGACAGCGG GTCTTCACCC TCTTCATTAT CAGCTTCAAG 
TTCACCTTCT TGGTCTCCAT CCTGATCTAT TGGCACACAG TGGGTGCACC CAAGGACCAA 
AGACGACAGT ACAGCCTGCC AGTGGACTTC CCCTGCCCCC AGCTGGCCTT TCCTAGAGTC 
TCTGCCCCAG GCAACATCTT CTTCCTAGAG ACCTCCGACA GGACCAACCC CAGCTTTCTG 
TTTATGTGTT CTGTGGAATC AGCTGCCAGG GCACACCCTG AGTCCCAGGT GGTGGTGCTC 
ATGAAAGGGC TGCCTAGGGA CACTACCGCA TGGCCCCGGA ACCTTGGCAT CTCTCTCCTG 
AGCTGCTTTC CCAATGTCCA GATACGACCT TTGGACCTGC AAGAACTGTT TGAGGACACG 
CCACTGGCAG CCTGGTACTT GGAGGCACAG CACAGGTGGG AACCCTACCT GTTGCCGGTG 
CTGTCTGACG CTTCCAGGAT CGCACTCCTC TGGAAGTTTG GTGGCATCTA CTTGGACACA 
GACTTCATCG TCCTCAAGAA CTTACGCAAC CTGACCAACA TGCTGGGCAT CCAGTCCCGC 
TACGTCCTCA ATGGCGCCTT CCTAGCCTTC GAGCGTAAGC ACGAGTTCCT GGCACTGTGC 
ATACGTGACT TTGTGGCCCA TTACAATGGT TGGATTTGGG GCCACCAGGG CCCCCAACTG 
CTTACCCGAG TCTTCAAGAA GTGGTGTTCC ATCCACAGCC TGAAGGAGAG CCGAGCTTGC 
CGCGGGGTCA CTGCCCTGCC TCCCGAGGCC TTTTACCCCA TCCCCTGGCA GAACTGGAAG 
AAATACTTTG AAGACGTCAG CCCCGAGGAG CTAGCCCAGC TGCTCAATGC CACCTATGCT 
GTTCATGTGT GGAACAAGAA AAGTCAGGGT ACACATCTAG AGGCCACATC CAGGGCTCTG 
CTGGCTCAGC TACATGCCCG CTACTGCCCT ACGACGCACA GAGCCATGAC GATGTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

A4galt ( XM_017595078.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450567.1 Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62138
Clone ID Related Accession (Same CDS sequence)	XM_017595078.1
Accession Version	XM_017595078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lactosylceramide 4-alpha-galactosyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGTG GGCTTGGCAG GCCTGAGCAT GAGAGGACAG TCCATAAATT CCTGTTCGCA 
CAGAAGATCA GCTGCTCACC CAGTGCACAT GTTCAGGATG CCTGCAGGCT GCTAGGACCC 
CCAACCTCCT TGTCATGGAC TGCCCAGGCT GACAAGTCAA TCCTTATGGG CATCTCCCGT 
TCGGATCTGG AGGAGACCAT GTCCAAGCCC CCCGACTGCC TGCCTCGGAT GCTCAGGGGC 
ACGCCCAGAC AGCGGGTCTT CACCCTCTTC ATTATCAGCT TCAAGTTCAC CTTCTTGGTC 
TCCATCCTGA TCTATTGGCA CACAGTGGGT GCACCCAAGG ACCAAAGACG ACAGTACAGC 
CTGCCAGTGG ACTTCCCCTG CCCCCAGCTG GCCTTTCCTA GAGTCTCTGC CCCAGGCAAC 
ATCTTCTTCC TAGAGACCTC CGACAGGACC AACCCCAGCT TTCTGTTTAT GTGTTCTGTG 
GAATCAGCTG CCAGGGCACA CCCTGAGTCC CAGGTGGTGG TGCTCATGAA AGGGCTGCCT 
AGGGACACTA CCGCATGGCC CCGGAACCTT GGCATCTCTC TCCTGAGCTG CTTTCCCAAT 
GTCCAGATAC GACCTTTGGA CCTGCAAGAA CTGTTTGAGG ACACGCCACT GGCAGCCTGG 
TACTTGGAGG CACAGCACAG GTGGGAACCC TACCTGTTGC CGGTGCTGTC TGACGCTTCC 
AGGATCGCAC TCCTCTGGAA GTTTGGTGGC ATCTACTTGG ACACAGACTT CATCGTCCTC 
AAGAACTTAC GCAACCTGAC CAACATGCTG GGCATCCAGT CCCGCTACGT CCTCAATGGC 
GCCTTCCTAG CCTTCGAGCG TAAGCACGAG TTCCTGGCAC TGTGCATACG TGACTTTGTG 
GCCCATTACA ATGGTTGGAT TTGGGGCCAC CAGGGCCCCC AACTGCTTAC CCGAGTCTTC 
AAGAAGTGGT GTTCCATCCA CAGCCTGAAG GAGAGCCGAG CTTGCCGCGG GGTCACTGCC 
CTGCCTCCCG AGGCCTTTTA CCCCATCCCC TGGCAGAACT GGAAGAAATA CTTTGAAGAC 
GTCAGCCCCG AGGAGCTAGC CCAGCTGCTC AATGCCACCT ATGCTGTTCA TGTGTGGAAC 
AAGAAAAGTC AGGGTACACA TCTAGAGGCC ACATCCAGGG CTCTGCTGGC TCAGCTACAT 
GCCCGCTACT GCCCTACGAC GCACAGAGCC ATGACGATGT ACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450567.1
CDS	541..1788
Translation	MKGGLGRPEHERTVHKFLFAQKISCSPSAHVQDACRLLGPPTSLSWTAQADKSILMGISRSDLEETMSKPPDCLPRMLRGTPRQRVFTLFIISFKFTFLVSILIYWHTVGAPKDQRRQYSLPVDFPCPQLAFPRVSAPGNIFFLETSDRTNPSFLFMCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTPLAAWYLEAQHRWEPYLLPVLSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGIQSRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCSIHSLKESRACRGVTALPPEAFYPIPWQNWKKYFEDVSPEELAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYCPTTHRAMTMYL

Target ORF information:

RefSeq Version	XM_017595078.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha 1,4-galactosyltransferase (A4galt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595078.1

ORF Insert Sequence:

ATGAAAGGTG GGCTTGGCAG GCCTGAGCAT GAGAGGACAG TCCATAAATT CCTGTTCGCA 
CAGAAGATCA GCTGCTCACC CAGTGCACAT GTTCAGGATG CCTGCAGGCT GCTAGGACCC 
CCAACCTCCT TGTCATGGAC TGCCCAGGCT GACAAGTCAA TCCTTATGGG CATCTCCCGT 
TCGGATCTGG AGGAGACCAT GTCCAAGCCC CCCGACTGCC TGCCTCGGAT GCTCAGGGGC 
ACGCCCAGAC AGCGGGTCTT CACCCTCTTC ATTATCAGCT TCAAGTTCAC CTTCTTGGTC 
TCCATCCTGA TCTATTGGCA CACAGTGGGT GCACCCAAGG ACCAAAGACG ACAGTACAGC 
CTGCCAGTGG ACTTCCCCTG CCCCCAGCTG GCCTTTCCTA GAGTCTCTGC CCCAGGCAAC 
ATCTTCTTCC TAGAGACCTC CGACAGGACC AACCCCAGCT TTCTGTTTAT GTGTTCTGTG 
GAATCAGCTG CCAGGGCACA CCCTGAGTCC CAGGTGGTGG TGCTCATGAA AGGGCTGCCT 
AGGGACACTA CCGCATGGCC CCGGAACCTT GGCATCTCTC TCCTGAGCTG CTTTCCCAAT 
GTCCAGATAC GACCTTTGGA CCTGCAAGAA CTGTTTGAGG ACACGCCACT GGCAGCCTGG 
TACTTGGAGG CACAGCACAG GTGGGAACCC TACCTGTTGC CGGTGCTGTC TGACGCTTCC 
AGGATCGCAC TCCTCTGGAA GTTTGGTGGC ATCTACTTGG ACACAGACTT CATCGTCCTC 
AAGAACTTAC GCAACCTGAC CAACATGCTG GGCATCCAGT CCCGCTACGT CCTCAATGGC 
GCCTTCCTAG CCTTCGAGCG TAAGCACGAG TTCCTGGCAC TGTGCATACG TGACTTTGTG 
GCCCATTACA ATGGTTGGAT TTGGGGCCAC CAGGGCCCCC AACTGCTTAC CCGAGTCTTC 
AAGAAGTGGT GTTCCATCCA CAGCCTGAAG GAGAGCCGAG CTTGCCGCGG GGTCACTGCC 
CTGCCTCCCG AGGCCTTTTA CCCCATCCCC TGGCAGAACT GGAAGAAATA CTTTGAAGAC 
GTCAGCCCCG AGGAGCTAGC CCAGCTGCTC AATGCCACCT ATGCTGTTCA TGTGTGGAAC 
AAGAAAAGTC AGGGTACACA TCTAGAGGCC ACATCCAGGG CTCTGCTGGC TCAGCTACAT 
GCCCGCTACT GCCCTACGAC GCACAGAGCC ATGACGATGT ACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Globosides but not isoglobosides can impact the development of invariant NKT cells and their interaction with dendritic cells.
Journal of immunology (Baltimore, Md. : 1950)189(6)3007-17(2012 Sep)
Porubsky S,Speak AO,Salio M,Jennemann R,Bonrouhi M,Zafarulla R,Singh Y,Dyson J,Luckow B,Lehuen A,Malle E,M?thing J,Platt FM,Cerundolo V,Gr?ne HJ

Targeted disruption of Gb3/CD77 synthase gene resulted in the complete deletion of globo-series glycosphingolipids and loss of sensitivity to verotoxins.
The Journal of biological chemistry281(15)10230-5(2006 Apr)
Okuda T,Tokuda N,Numata S,Ito M,Ohta M,Kawamura K,Wiels J,Urano T,Tajima O,Furukawa K,Furukawa K

Cloning of Gb3 synthase, the key enzyme in globo-series glycosphingolipid synthesis, predicts a family of alpha 1, 4-glycosyltransferases conserved in plants, insects, and mammals.
The Journal of biological chemistry275(33)25315-21(2000 Aug)
Keusch JJ,Manzella SM,Nyame KA,Cummings RD,Baenziger JU

Cloning and expression of the histo-blood group Pk UDP-galactose: Ga1beta-4G1cbeta1-cer alpha1, 4-galactosyltransferase. Molecular genetic basis of the p phenotype.
The Journal of biological chemistry275(22)16723-9(2000 Jun)
Steffensen R,Carlier K,Wiels J,Levery SB,Stroud M,Cedergren B,Nilsson Sojka B,Bennett EP,Jersild C,Clausen H

Molecular cloning of globotriaosylceramide/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids.
The Journal of biological chemistry275(20)15152-6(2000 May)
Kojima Y,Fukumoto S,Furukawa K,Okajima T,Wiels J,Yokoyama K,Suzuki Y,Urano T,Ohta M,Furukawa K

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A4galt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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