Nmu cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nmu
Entrez Gene ID	63887
Full Name	neuromedin U
General protein information	Preferred Names neuromedin U Names neuromedin-U neuromedin U precursor-related peptide/neuromedin U preproprotein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p11
Summary	may induce uterine smooth muscle contraction and selective vasoconstriction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006250872.3	XP_006250934.1	neuromedin-U isoform X1
NM_022239.2	NP_071575.1	neuromedin-U precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nmu	NP_062388.1
Danio rerio (zebrafish)	nmu	XP_005160570.1
Homo sapiens (human)	NMU	NP_006672.1
Canis lupus familiaris (dog)	NMU	XP_005628261.1
Bos taurus (cattle)	NMU	NP_001178082.1
Gallus gallus (chicken)	NMU	NP_001264850.1
Xenopus tropicalis (tropical clawed frog)	nmu	XP_004911525.1
Rattus norvegicus (Norway rat)	Nmu	NP_071575.1
Pan troglodytes (chimpanzee)	NMU	XP_001142975.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of neuromedin U precursor-related peptide and its possible role in the regulation of prolactin release.
Mori K, Ida T, Fudetani M, Mori M, Kaiya H, Hino J, Nakahara K, Murakami N, Miyazato M, Kangawa K
Scientific reports7(1)10468(2017 Sep)

PEGylated neuromedin U-8 shows long-lasting anorectic activity and anti-obesity effect in mice by peripheral administration.
Masuda Y, Kumano S, Noguchi J, Sakamoto K, Inooka H, Ohtaki T
Peptides9499-105(2017 Aug)

Involvement of endogenous neuromedin U and neuromedin S in thermoregulation.
Nakahara K, Akagi A, Shimizu S, Tateno S, Qattali AW, Mori K, Miyazato M, Kangawa K, Murakami N
Biochemical and biophysical research communications470(4)930-5(2016 Feb)

Mediators involved in the hyperthermic action of neuromedin U in rats.
Telegdy G, Adamik A
Regulatory peptides192-19324-9(2014 Jun-Aug)

Neuromedin U partly mimics thyroid-stimulating hormone and triggers Wnt/?-catenin signalling in the photoperiodic response of F344 rats.
Helfer G, Ross AW, Morgan PJ
Journal of neuroendocrinology25(12)1264-72(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that NMU and NMS are involved in thermoregulation via the prostaglandin E2 and beta3 adrenergic receptors, but that endogenous NMS might play a more predominant role than NMU.
Title: Involvement of endogenous neuromedin U and neuromedin S in thermoregulation.

The results demonstrated that, when injected into the lateral brain ventricle NmU increased the body temperature, mediated by CRHR1 and CRHR2, dopamine and muscarinic cholinergic receptors.
Title: Mediators involved in the hyperthermic action of neuromedin U in rats.

study of NMU signalling reveals the potential for a new player in the control of seasonal biology
Title: Neuromedin U partly mimics thyroid-stimulating hormone and triggers Wnt/?-catenin signalling in the photoperiodic response of F344 rats.

Functional evidence that NMU can have an important effect on the cardiovascular and reflex responses that are integrated in the rostral ventrolateral medulla.
Title: Neuromedin U causes biphasic cardiovascular effects and impairs baroreflex function in rostral ventrolateral medulla of spontaneously hypertensive rat.

Melatonin negatively regulates neuromedin U mRNA expression in the rat pars tuberalis.
Title: Negative regulation of neuromedin U mRNA expression in the rat pars tuberalis by melatonin.

The following Nmu gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nmu cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05511	NM_022239.2 Latest version!	Rattus norvegicus neuromedin U (Nmu), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa36003	XM_006250872.3 Latest version!	Rattus norvegicus neuromedin U (Nmu), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nmu ( NM_022239.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071575.1 Rattus norvegicus neuromedin U (Nmu), mRNA.

Price & Availability↑

CloneID	ORa05511
Clone ID Related Accession (Same CDS sequence)	NM_022239.2
Accession Version	NM_022239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	neuromedin-U precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M94555.1. On Jun 10, 2007 this sequence version replaced NM_022239.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M94555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCGAG CAGCTAATCG CCGCCCAGGG CTCTCCGCAG GACAATTGGC CGCTGCAACT 
GCATCTCCTC TCCTGTCACT GCTGCTGCTG CTCGCCTGCT GTGCGGACGC TTGCAGAGGT 
ACGCCAATAT CGCCTCAAAG ATTACCACCA GAACAAGAAC TACAATTGTG GAATGAGATT 
CCTGAAGCTT GTGCGTCCTT TCTGTCTGTT GATTCCCAGC CTCAGGCATC TGTTGCATTG 
AGGAAGCTTT GCCGTGTCCT GATGGAGATT TTCCAGAAGC CTCAGGAGCA AACTGAGAAA 
GATAATGCTA AGAGGTTCTT ATTTCATTAT TCAAAGACAC AGAAGTTGGG AAATTCAAAT 
GTTGTGTCGT CTGTCGTGCA TCCGTTGCTG CAGCTCGTTC CTCAACTGCA TGAGAGAAGA 
ATGAAGAGAT ACAAAGTGAA TGAATACCAG GGTCCTGTAG CTCCAAGTGG GGGATTCTTT 
TTATTTAGGC CACGCAATGG GAAGAGGTCA ACAAGTTTCA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071575.1
CDS	113..637
Translation	MSRAANRRPGLSAGQLAAATASPLLSLLLLLACCADACRGTPISPQRLPPEQELQLWNEIPEACASFLSVDSQPQASVALRKLCRVLMEIFQKPQEQTEKDNAKRFLFHYSKTQKLGNSNVVSSVVHPLLQLVPQLHERRMKRYKVNEYQGPVAPSGGFFLFRPRNGKRSTSFI

Target ORF information:

RefSeq Version	NM_022239.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuromedin U (Nmu), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022239.2

ORF Insert Sequence:

ATGTCGCGAG CAGCTAATCG CCGCCCAGGG CTCTCCGCAG GACAATTGGC CGCTGCAACT 
GCATCTCCTC TCCTGTCACT GCTGCTGCTG CTCGCCTGCT GTGCGGACGC TTGCAGAGGT 
ACGCCAATAT CGCCTCAAAG ATTACCACCA GAACAAGAAC TACAATTGTG GAATGAGATT 
CCTGAAGCTT GTGCGTCCTT TCTGTCTGTT GATTCCCAGC CTCAGGCATC TGTTGCATTG 
AGGAAGCTTT GCCGTGTCCT GATGGAGATT TTCCAGAAGC CTCAGGAGCA AACTGAGAAA 
GATAATGCTA AGAGGTTCTT ATTTCATTAT TCAAAGACAC AGAAGTTGGG AAATTCAAAT 
GTTGTGTCGT CTGTCGTGCA TCCGTTGCTG CAGCTCGTTC CTCAACTGCA TGAGAGAAGA 
ATGAAGAGAT ACAAAGTGAA TGAATACCAG GGTCCTGTAG CTCCAAGTGG GGGATTCTTT 
TTATTTAGGC CACGCAATGG GAAGAGGTCA ACAAGTTTCA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nmu ( XM_006250872.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250934.1 Rattus norvegicus neuromedin U (Nmu), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa36003
Clone ID Related Accession (Same CDS sequence)	XM_006250872.3
Accession Version	XM_006250872.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuromedin-U isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250872.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGAG CAGCTAATCG CCGCCCAGGG CTCTCCGCAG GACAATTGGC CGCTGCAACT 
GCATCTCCTC TCCTGTCACT GCTGCTGCTG CTCGCCTGCT GTGCGGACGC TTGCAGAGGT 
ACGCCAATAT CGCCTCAAAG ATTACCACCA GAACAAGAAC TACAATTGTG GAATGAGATT 
CCTGAAGCTT GTGCGTCCTT TCTGTCTGTT GATTCCCAGC CTCAGGCATC TGTTGCATTG 
AGGAAGCTTT GCCGTGTCCT GATGGAGATT TTCCAGAAGC CTCAGTTCTT ATTTCATTAT 
TCAAAGACAC AGAAGTTGGG AAATTCAAAT GTTGTGTCGT CTGTCGTGCA TCCGTTGCTG 
CAGCTCGTTC CTCAACTGCA TGAGAGAAGA ATGAAGAGAT ACAAAGTGAA TGAATACCAG 
GGTCCTGTAG CTCCAAGTGG GGGATTCTTT TTATTTAGGC CACGCAATGG GAAGAGGTCA 
ACAAGTTTCA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250934.1
CDS	933..1427
Translation	MSRAANRRPGLSAGQLAAATASPLLSLLLLLACCADACRGTPISPQRLPPEQELQLWNEIPEACASFLSVDSQPQASVALRKLCRVLMEIFQKPQFLFHYSKTQKLGNSNVVSSVVHPLLQLVPQLHERRMKRYKVNEYQGPVAPSGGFFLFRPRNGKRSTSFI

Target ORF information:

RefSeq Version	XM_006250872.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuromedin U (Nmu), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250872.3

ORF Insert Sequence:

ATGTCGCGAG CAGCTAATCG CCGCCCAGGG CTCTCCGCAG GACAATTGGC CGCTGCAACT 
GCATCTCCTC TCCTGTCACT GCTGCTGCTG CTCGCCTGCT GTGCGGACGC TTGCAGAGGT 
ACGCCAATAT CGCCTCAAAG ATTACCACCA GAACAAGAAC TACAATTGTG GAATGAGATT 
CCTGAAGCTT GTGCGTCCTT TCTGTCTGTT GATTCCCAGC CTCAGGCATC TGTTGCATTG 
AGGAAGCTTT GCCGTGTCCT GATGGAGATT TTCCAGAAGC CTCAGTTCTT ATTTCATTAT 
TCAAAGACAC AGAAGTTGGG AAATTCAAAT GTTGTGTCGT CTGTCGTGCA TCCGTTGCTG 
CAGCTCGTTC CTCAACTGCA TGAGAGAAGA ATGAAGAGAT ACAAAGTGAA TGAATACCAG 
GGTCCTGTAG CTCCAAGTGG GGGATTCTTT TTATTTAGGC CACGCAATGG GAAGAGGTCA 
ACAAGTTTCA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of neuromedin U precursor-related peptide and its possible role in the regulation of prolactin release.
Scientific reports7(1)10468(2017 Sep)
Mori K,Ida T,Fudetani M,Mori M,Kaiya H,Hino J,Nakahara K,Murakami N,Miyazato M,Kangawa K

PEGylated neuromedin U-8 shows long-lasting anorectic activity and anti-obesity effect in mice by peripheral administration.
Peptides9499-105(2017 Aug)
Masuda Y,Kumano S,Noguchi J,Sakamoto K,Inooka H,Ohtaki T

Involvement of endogenous neuromedin U and neuromedin S in thermoregulation.
Biochemical and biophysical research communications470(4)930-5(2016 Feb)
Nakahara K,Akagi A,Shimizu S,Tateno S,Qattali AW,Mori K,Miyazato M,Kangawa K,Murakami N

Mediators involved in the hyperthermic action of neuromedin U in rats.
Regulatory peptides192-19324-9(2014 Jun-Aug)
Telegdy G,Adamik A

Neuromedin U partly mimics thyroid-stimulating hormone and triggers Wnt/?-catenin signalling in the photoperiodic response of F344 rats.
Journal of neuroendocrinology25(12)1264-72(2013 Dec)
Helfer G,Ross AW,Morgan PJ