Hspa2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspa2
Entrez Gene ID	60460
Full Name	heat shock protein family A member 2
Synonyms	Hspt70,Hst70
General protein information	Preferred Names heat shock protein family A member 2 Names heat shock-related 70 kDa protein 2 HST heat shock 70kDa protein 2 heat shock protein 2 heat shock protein 70.2 heat shock protein alpha 2 testis-specific heat shock protein-related gene hst70
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24
Summary	involved in repeated binding and release of substrate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021863.3	NP_068635.1	heat shock-related 70 kDa protein 2
XM_006240247.3	XP_006240309.1	heat shock-related 70 kDa protein 2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HSPA2	XP_510002.3
Mus musculus (house mouse)	Hspa2	NP_032327.2
Arabidopsis thaliana (thale cress)	ERD2	NP_176036.1
Rattus norvegicus (Norway rat)	Hspa2	NP_068635.1
Xenopus tropicalis (tropical clawed frog)	hspa2	XP_004917261.1
Homo sapiens (human)	HSPA2	NP_068814.2
Canis lupus familiaris (dog)	HSPA2	XP_537479.1
Bos taurus (cattle)	HSPA2	NP_776769.1
Gallus gallus (chicken)	HSPA2	NP_001006686.1

Gene Ontology
Bibliography

Related articles in PubMed

Evaluation of heat-shock protein A2 (HSPA2) in male rats before and after varicocele induction.
Afiyani AA, Deemeh MR, Tavalaee M, Razi M, Bahadorani M, Shokrollahi B, Nasr-Esfahani MH
Molecular reproduction and development81(8)766-76(2014 Aug)

SHCBP1L, a conserved protein in mammals, is predominantly expressed in male germ cells and maintains spindle stability during meiosis in testis.
Liu M, Shi X, Bi Y, Qi L, Guo X, Wang L, Zhou Z, Sha J
Molecular human reproduction20(6)463-75(2014 Jun)

Identification and characterization of a novel human methyltransferase modulating Hsp70 protein function through lysine methylation.
Jakobsson ME, Moen A, Bousset L, Egge-Jacobsen W, Kernstock S, Melki R, Falnes P?
The Journal of biological chemistry288(39)27752-63(2013 Sep)

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Bastos-Amador P, Royo F, Gonzalez E, Conde-Vancells J, Palomo-Diez L, Borras FE, Falcon-Perez JM
Journal of proteomics75(12)3574-84(2012 Jun)

HSP70-2 is required for desynapsis of synaptonemal complexes during meiotic prophase in juvenile and adult mouse spermatocytes.
Dix DJ, Allen JW, Collins BW, Poorman-Allen P, Mori C, Blizard DR, Brown PR, Goulding EH, Strong BD, Eddy EM
Development (Cambridge, England)124(22)4595-603(1997 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HSPA2 abundance increased whereas ubiquitin expression reduced in the affected testis of a rat varicocele model.
Title: Evaluation of heat-shock protein A2 (HSPA2) in male rats before and after varicocele induction.

high-level testis-specific gene expression requires additional sequences localized upstream of the T2 transcription start site
Title: Location of promoter elements necessary and sufficient to direct testis-specific expression of the Hst70/Hsp70.2 gene.

The following Hspa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa29428	XM_006240247.3 Latest version!	Rattus norvegicus heat shock protein family A member 2 (Hspa2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa42673	NM_021863.3 Latest version!	Rattus norvegicus heat shock protein family A (Hsp70) member 2 (Hspa2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspa2 ( XM_006240247.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240309.1 Rattus norvegicus heat shock protein family A member 2 (Hspa2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29428
Clone ID Related Accession (Same CDS sequence)	XM_006240247.3
Accession Version	XM_006240247.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	heat shock-related 70 kDa protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240247.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCC GCGGCCCGGC TATCGGCATC GACCTGGGCA CCACTTACTC GTGCGTGGGG 
GTCTTCCAAC ATGGCAAGGT GGAGATCATC GCCAACGACC AGGGTAACCG GACCACCCCC 
AGCTACGTGG CCTTCACCGA CACCGAGCGC CTTATCGGCG ACGCCGCCAA GAACCAAGTG 
GCCATGAACC CCACAAACAC CATCTTCGAC GCCAAGCGGC TGATCGGACG GAAGTTCGAA 
GACGCCACGG TGCAGTCGGA TATGAAGCAC TGGCCGTTCA GAGTGGTGAG CGAAGGTGGG 
AAGCCCAAAG TGCAGGTGGA ATATAAAGGG GAGATGAAGA CCTTCTTCCC TGAGGAGATT 
TCTTCCATGG TCCTCACTAA GATGAAGGAG ATCGCCGAAG CCTACCTGGG GGGCAAGGTG 
CAGAGCGCAG TCATCACTGT TCCTGCCTAT TTCAACGACT CGCAGCGCCA GGCCACCAAG 
GATGCGGGCA CCATCACCGG TCTCAATGTA TTGCGTATCA TCAACGAACC CACAGCAGCG 
GCCATCGCTT ATGGCCTGGA TAAGAAGGGC TGCGCGGGCG GCGAGAAGAA CGTGCTCATC 
TTTGACCTGG GCGGGGGCAC CTTCGATGTG TCCATCCTCA CCATCGAGGA TGGCATCTTC 
GAGGTCAAGT CCACGGCCGG CGACACCCAC CTGGGTGGCG AAGACTTCGA CAACCGTATG 
GTCAGCCACC TGGCGGAGGA GTTCAAGCGC AAACACAAGA AGGACATTGG GCCCAACAAG 
CGCGCCGTGC GCCGGCTGCG CACCGCCTGC GAGCGCGCTA AGCGCACCCT GAGCTCGTCC 
ACGCAGGCCA GCATCGAGAT CGACTCGCTG TACGAGGGCG TGGATTTCTA CACGTCCATC 
ACCCGCGCCC GCTTCGAGGA GCTCAACGCC GATCTCTTTC GAGGGACTCT GGAGCCGGTG 
GAAAAGGCGC TGCGCGATGC CAAGCTAGAC AAGGGCCAGA TCCAGGAGAT AGTGCTGGTG 
GGCGGCTCTA CCCGCATCCC TAAGATCCAG AAGCTCCTGC AGGATTTCTT CAACGGCAAG 
GAGCTGAACA AGAGCATCAA TCCCGACGAG GCGGTGGCCT ACGGCGCCGC CGTGCAGGCG 
GCTATCCTCA TTGGCGACAA GTCTGAGAAC GTGCAGGATC TGCTGTTACT CGACGTGACT 
CCCTTGTCGC TTGGCATTGA AACAGCTGGC GGTGTCATGA CCCCACTCAT AAAGAGAAAC 
ACCACCATCC CTACCAAGCA GACGCAGACC TTCACTACCT ACTCAGACAA CCAGAGCAGC 
GTGCTGGTGC AAGTGTACGA GGGCGAACGG GCCATGACCA AGGACAATAA CCTCTTGGGC 
AAGTTCGACC TAACTGGGAT CCCTCCAGCA CCCCGCGGGG TCCCCCAGAT CGAGGTCACC 
TTTGACATCG ATGCCAACGG CATTCTTAAC GTCACTGCTG CAGACAAGAG CACCGGCAAA 
GAAAATAAAA TCACCATCAC CAACGACAAG GGTCGTCTGA GCAAAGACGA CATTGACCGG 
ATGGTGCAGG AGGCAGAGCG GTACAAATCG GAAGATGAAG CTAATCGCGA TCGCGTGGCA 
GCCAAAAACG CGGTTGAGTC CTATACCTAC AACATCAAGC AGACGGTGGA AGACGAGAAA 
CTGAGGGGCA AGATTAGCGA GCAGGACAAA AACAAGATTC TCGACAAGTG TCAGGAGGTG 
ATCAACTGGC TTGACCGAAA CCAGATGGCA GAGAAAGATG AGTACGAACA CAAGCAGAAA 
GAGCTTGAGA GAGTGTGCAA CCCCATCATC AGCAAACTTT ACCAAGGCGG TCCAGGCGGC 
GGCGGCTCCT CTGGAGGACC CACCATTGAG GAAGTGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240309.1
CDS	85..1986
Translation	MSARGPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFDAKRLIGRKFEDATVQSDMKHWPFRVVSEGGKPKVQVEYKGEMKTFFPEEISSMVLTKMKEIAEAYLGGKVQSAVITVPAYFNDSQRQATKDAGTITGLNVLRIINEPTAAAIAYGLDKKGCAGGEKNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLAEEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNAVESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGGSSGGPTIEEVD

Target ORF information:

RefSeq Version	XM_006240247.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heat shock protein family A member 2 (Hspa2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240247.3

ORF Insert Sequence:

ATGTCCGCCC GCGGCCCGGC TATCGGCATC GACCTGGGCA CCACTTACTC GTGCGTGGGG 
GTCTTCCAAC ATGGCAAGGT GGAGATCATC GCCAACGACC AGGGTAACCG GACCACCCCC 
AGCTACGTGG CCTTCACCGA CACCGAGCGC CTTATCGGCG ACGCCGCCAA GAACCAAGTG 
GCCATGAACC CCACAAACAC CATCTTCGAC GCCAAGCGGC TGATCGGACG GAAGTTCGAA 
GACGCCACGG TGCAGTCGGA TATGAAGCAC TGGCCGTTCA GAGTGGTGAG CGAAGGTGGG 
AAGCCCAAAG TGCAGGTGGA ATATAAAGGG GAGATGAAGA CCTTCTTCCC TGAGGAGATT 
TCTTCCATGG TCCTCACTAA GATGAAGGAG ATCGCCGAAG CCTACCTGGG GGGCAAGGTG 
CAGAGCGCAG TCATCACTGT TCCTGCCTAT TTCAACGACT CGCAGCGCCA GGCCACCAAG 
GATGCGGGCA CCATCACCGG TCTCAATGTA TTGCGTATCA TCAACGAACC CACAGCAGCG 
GCCATCGCTT ATGGCCTGGA TAAGAAGGGC TGCGCGGGCG GCGAGAAGAA CGTGCTCATC 
TTTGACCTGG GCGGGGGCAC CTTCGATGTG TCCATCCTCA CCATCGAGGA TGGCATCTTC 
GAGGTCAAGT CCACGGCCGG CGACACCCAC CTGGGTGGCG AAGACTTCGA CAACCGTATG 
GTCAGCCACC TGGCGGAGGA GTTCAAGCGC AAACACAAGA AGGACATTGG GCCCAACAAG 
CGCGCCGTGC GCCGGCTGCG CACCGCCTGC GAGCGCGCTA AGCGCACCCT GAGCTCGTCC 
ACGCAGGCCA GCATCGAGAT CGACTCGCTG TACGAGGGCG TGGATTTCTA CACGTCCATC 
ACCCGCGCCC GCTTCGAGGA GCTCAACGCC GATCTCTTTC GAGGGACTCT GGAGCCGGTG 
GAAAAGGCGC TGCGCGATGC CAAGCTAGAC AAGGGCCAGA TCCAGGAGAT AGTGCTGGTG 
GGCGGCTCTA CCCGCATCCC TAAGATCCAG AAGCTCCTGC AGGATTTCTT CAACGGCAAG 
GAGCTGAACA AGAGCATCAA TCCCGACGAG GCGGTGGCCT ACGGCGCCGC CGTGCAGGCG 
GCTATCCTCA TTGGCGACAA GTCTGAGAAC GTGCAGGATC TGCTGTTACT CGACGTGACT 
CCCTTGTCGC TTGGCATTGA AACAGCTGGC GGTGTCATGA CCCCACTCAT AAAGAGAAAC 
ACCACCATCC CTACCAAGCA GACGCAGACC TTCACTACCT ACTCAGACAA CCAGAGCAGC 
GTGCTGGTGC AAGTGTACGA GGGCGAACGG GCCATGACCA AGGACAATAA CCTCTTGGGC 
AAGTTCGACC TAACTGGGAT CCCTCCAGCA CCCCGCGGGG TCCCCCAGAT CGAGGTCACC 
TTTGACATCG ATGCCAACGG CATTCTTAAC GTCACTGCTG CAGACAAGAG CACCGGCAAA 
GAAAATAAAA TCACCATCAC CAACGACAAG GGTCGTCTGA GCAAAGACGA CATTGACCGG 
ATGGTGCAGG AGGCAGAGCG GTACAAATCG GAAGATGAAG CTAATCGCGA TCGCGTGGCA 
GCCAAAAACG CGGTTGAGTC CTATACCTAC AACATCAAGC AGACGGTGGA AGACGAGAAA 
CTGAGGGGCA AGATTAGCGA GCAGGACAAA AACAAGATTC TCGACAAGTG TCAGGAGGTG 
ATCAACTGGC TTGACCGAAA CCAGATGGCA GAGAAAGATG AGTACGAACA CAAGCAGAAA 
GAGCTTGAGA GAGTGTGCAA CCCCATCATC AGCAAACTTT ACCAAGGCGG TCCAGGCGGC 
GGCGGCTCCT CTGGAGGACC CACCATTGAG GAAGTGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa2 ( NM_021863.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068635.1 Rattus norvegicus heat shock protein family A (Hsp70) member 2 (Hspa2), mRNA.

Price & Availability↑

CloneID	ORa42673
Clone ID Related Accession (Same CDS sequence)	NM_021863.3
Accession Version	NM_021863.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	heat shock-related 70 kDa protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X15705.2. On Apr 20, 2006 this sequence version replaced NM_021863.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGTCCGCCC GCGGCCCGGC TATCGGCATC GACCTGGGCA CCACTTACTC GTGCGTGGGG 
GTCTTCCAAC ATGGCAAGGT GGAGATCATC GCCAACGACC AGGGTAACCG GACCACCCCC 
AGCTACGTGG CCTTCACCGA CACCGAGCGC CTTATCGGCG ACGCCGCCAA GAACCAAGTG 
GCCATGAACC CCACAAACAC CATCTTCGAC GCCAAGCGGC TGATCGGACG GAAGTTCGAA 
GACGCCACGG TGCAGTCGGA TATGAAGCAC TGGCCGTTCA GAGTGGTGAG CGAAGGTGGG 
AAGCCCAAAG TGCAGGTGGA ATATAAAGGG GAGATGAAGA CCTTCTTCCC TGAGGAGATT 
TCTTCCATGG TCCTCACTAA GATGAAGGAG ATCGCCGAAG CCTACCTGGG GGGCAAGGTG 
CAGAGCGCAG TCATCACTGT TCCTGCCTAT TTCAACGACT CGCAGCGCCA GGCCACCAAG 
GATGCGGGCA CCATCACCGG TCTCAATGTA TTGCGTATCA TCAACGAACC CACAGCAGCG 
GCCATCGCTT ATGGCCTGGA TAAGAAGGGC TGCGCGGGCG GCGAGAAGAA CGTGCTCATC 
TTTGACCTGG GCGGGGGCAC CTTCGATGTG TCCATCCTCA CCATCGAGGA TGGCATCTTC 
GAGGTCAAGT CCACGGCCGG CGACACCCAC CTGGGTGGCG AAGACTTCGA CAACCGTATG 
GTCAGCCACC TGGCGGAGGA GTTCAAGCGC AAACACAAGA AGGACATTGG GCCCAACAAG 
CGCGCTGTGC GCCGGCTGCG CACCGCCTGC GAGCGCGCTA AGCGCACCCT GAGCTCGTCC 
ACGCAGGCCA GCATCGAGAT CGACTCGCTG TACGAGGGCG TGGATTTCTA CACGTCCATC 
ACCCGCGCCC GCTTCGAGGA GCTCAACGCC GATCTCTTTC GAGGGACTCT GGAGCCGGTG 
GAAAAGGCGC TGCGCGATGC CAAGCTAGAC AAGGGCCAGA TCCAGGAGAT AGTGCTGGTG 
GGCGGCTCTA CCCGCATCCC TAAGATCCAG AAGCTCCTGC AGGATTTCTT CAACGGCAAG 
GAGCTGAACA AGAGCATCAA TCCCGACGAG GCGGTGGCCT ACGGCGCCGC TGTGCAGGCG 
GCTATCCTCA TTGGCGACAA GTCTGAGAAC GTGCAGGATC TGCTGTTACT CGACGTGACT 
CCCTTGTCGC TTGGCATTGA AACAGCTGGC GGTGTCATGA CCCCACTCAT AAAGAGAAAC 
ACCACCATCC CTACCAAGCA GACGCAGACC TTCACTACCT ACTCAGACAA CCAGAGCAGC 
GTGCTGGTGC AAGTGTACGA GGGCGAACGG GCCATGACCA AGGACAATAA CCTCTTGGGC 
AAGTTCGACC TAACTGGGAT CCCTCCAGCA CCCCGCGGCG TCCCCCAGAT CGAGGTCACC 
TTTGACATCG ATGCCAACGG CATTCTTAAC GTCACTGCTG CAGACAAGAG CACCGGCAAA 
GAAAATAAAA TCACCATCAC CAACGACAAG GGTCGTCTGA GCAAAGACGA CATTGACCGG 
ATGGTGCAGG AGGCAGACGG GTACAAATCG GAAGATGAAG CTAATCGCGA TCGCGTGGCA 
GCCAAAAACG CGGTTGAGTC CTATACCTAC AACATCAAGC AGACGGTGGA AGACGAGAAA 
CTGAGGGGCA AGATTAGCGA GCAGGACAAA AACAAGATTC TCGACAAGTG TCAGGAGGTG 
ATCAACTGGC TTGACCGAAA CCAGATGGCA GAGAAAGATG AGTACGAACA CAAGCAGAAA 
GAGCTTGAGA GAGTGTGCAA CCCCATCATC AGCAAACTTT ACCAAGGCGG TCCAGGCGGC 
GGCGGCTCCT CTGGAGGACC CACCATTGAG GAAGTGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068635.1
CDS	117..2018
Translation	MSARGPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFDAKRLIGRKFEDATVQSDMKHWPFRVVSEGGKPKVQVEYKGEMKTFFPEEISSMVLTKMKEIAEAYLGGKVQSAVITVPAYFNDSQRQATKDAGTITGLNVLRIINEPTAAAIAYGLDKKGCAGGEKNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLAEEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEADGYKSEDEANRDRVAAKNAVESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGGSSGGPTIEEVD

Target ORF information:

RefSeq Version	NM_021863.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heat shock protein family A (Hsp70) member 2 (Hspa2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021863.3

ORF Insert Sequence:

ATGTCCGCCC GCGGCCCGGC TATCGGCATC GACCTGGGCA CCACTTACTC GTGCGTGGGG 
GTCTTCCAAC ATGGCAAGGT GGAGATCATC GCCAACGACC AGGGTAACCG GACCACCCCC 
AGCTACGTGG CCTTCACCGA CACCGAGCGC CTTATCGGCG ACGCCGCCAA GAACCAAGTG 
GCCATGAACC CCACAAACAC CATCTTCGAC GCCAAGCGGC TGATCGGACG GAAGTTCGAA 
GACGCCACGG TGCAGTCGGA TATGAAGCAC TGGCCGTTCA GAGTGGTGAG CGAAGGTGGG 
AAGCCCAAAG TGCAGGTGGA ATATAAAGGG GAGATGAAGA CCTTCTTCCC TGAGGAGATT 
TCTTCCATGG TCCTCACTAA GATGAAGGAG ATCGCCGAAG CCTACCTGGG GGGCAAGGTG 
CAGAGCGCAG TCATCACTGT TCCTGCCTAT TTCAACGACT CGCAGCGCCA GGCCACCAAG 
GATGCGGGCA CCATCACCGG TCTCAATGTA TTGCGTATCA TCAACGAACC CACAGCAGCG 
GCCATCGCTT ATGGCCTGGA TAAGAAGGGC TGCGCGGGCG GCGAGAAGAA CGTGCTCATC 
TTTGACCTGG GCGGGGGCAC CTTCGATGTG TCCATCCTCA CCATCGAGGA TGGCATCTTC 
GAGGTCAAGT CCACGGCCGG CGACACCCAC CTGGGTGGCG AAGACTTCGA CAACCGTATG 
GTCAGCCACC TGGCGGAGGA GTTCAAGCGC AAACACAAGA AGGACATTGG GCCCAACAAG 
CGCGCTGTGC GCCGGCTGCG CACCGCCTGC GAGCGCGCTA AGCGCACCCT GAGCTCGTCC 
ACGCAGGCCA GCATCGAGAT CGACTCGCTG TACGAGGGCG TGGATTTCTA CACGTCCATC 
ACCCGCGCCC GCTTCGAGGA GCTCAACGCC GATCTCTTTC GAGGGACTCT GGAGCCGGTG 
GAAAAGGCGC TGCGCGATGC CAAGCTAGAC AAGGGCCAGA TCCAGGAGAT AGTGCTGGTG 
GGCGGCTCTA CCCGCATCCC TAAGATCCAG AAGCTCCTGC AGGATTTCTT CAACGGCAAG 
GAGCTGAACA AGAGCATCAA TCCCGACGAG GCGGTGGCCT ACGGCGCCGC TGTGCAGGCG 
GCTATCCTCA TTGGCGACAA GTCTGAGAAC GTGCAGGATC TGCTGTTACT CGACGTGACT 
CCCTTGTCGC TTGGCATTGA AACAGCTGGC GGTGTCATGA CCCCACTCAT AAAGAGAAAC 
ACCACCATCC CTACCAAGCA GACGCAGACC TTCACTACCT ACTCAGACAA CCAGAGCAGC 
GTGCTGGTGC AAGTGTACGA GGGCGAACGG GCCATGACCA AGGACAATAA CCTCTTGGGC 
AAGTTCGACC TAACTGGGAT CCCTCCAGCA CCCCGCGGCG TCCCCCAGAT CGAGGTCACC 
TTTGACATCG ATGCCAACGG CATTCTTAAC GTCACTGCTG CAGACAAGAG CACCGGCAAA 
GAAAATAAAA TCACCATCAC CAACGACAAG GGTCGTCTGA GCAAAGACGA CATTGACCGG 
ATGGTGCAGG AGGCAGACGG GTACAAATCG GAAGATGAAG CTAATCGCGA TCGCGTGGCA 
GCCAAAAACG CGGTTGAGTC CTATACCTAC AACATCAAGC AGACGGTGGA AGACGAGAAA 
CTGAGGGGCA AGATTAGCGA GCAGGACAAA AACAAGATTC TCGACAAGTG TCAGGAGGTG 
ATCAACTGGC TTGACCGAAA CCAGATGGCA GAGAAAGATG AGTACGAACA CAAGCAGAAA 
GAGCTTGAGA GAGTGTGCAA CCCCATCATC AGCAAACTTT ACCAAGGCGG TCCAGGCGGC 
GGCGGCTCCT CTGGAGGACC CACCATTGAG GAAGTGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evaluation of heat-shock protein A2 (HSPA2) in male rats before and after varicocele induction.
Molecular reproduction and development81(8)766-76(2014 Aug)
Afiyani AA,Deemeh MR,Tavalaee M,Razi M,Bahadorani M,Shokrollahi B,Nasr-Esfahani MH

SHCBP1L, a conserved protein in mammals, is predominantly expressed in male germ cells and maintains spindle stability during meiosis in testis.
Molecular human reproduction20(6)463-75(2014 Jun)
Liu M,Shi X,Bi Y,Qi L,Guo X,Wang L,Zhou Z,Sha J

Identification and characterization of a novel human methyltransferase modulating Hsp70 protein function through lysine methylation.
The Journal of biological chemistry288(39)27752-63(2013 Sep)
Jakobsson ME,Moen A,Bousset L,Egge-Jacobsen W,Kernstock S,Melki R,Falnes P?

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Journal of proteomics75(12)3574-84(2012 Jun)
Bastos-Amador P,Royo F,Gonzalez E,Conde-Vancells J,Palomo-Diez L,Borras FE,Falcon-Perez JM

HSP70-2 is required for desynapsis of synaptonemal complexes during meiotic prophase in juvenile and adult mouse spermatocytes.
Development (Cambridge, England)124(22)4595-603(1997 Nov)
Dix DJ,Allen JW,Collins BW,Poorman-Allen P,Mori C,Blizard DR,Brown PR,Goulding EH,Strong BD,Eddy EM

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Hspa2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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