Fhit cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fhit
Entrez Gene ID	60398
Full Name	fragile histidine triad
General protein information	Preferred Names fragile histidine triad Names bis(5'-adenosyl)-triphosphatase AP3A hydrolase AP3Aase diadenosine 5',5'''-P1,P3-triphosphate hydrolase dinucleosidetriphosphatase fragile histidine triad protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p15
Summary	may play a role in the development of hepatocellular carcinomas (HCCs) induced by N-nitrosodiethylamine (DEN) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021774.1	NP_068542.1	bis(5'-adenosyl)-triphosphatase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FHIT	XP_001156390.2
	FHIT	XP_001094642.1
Canis lupus familiaris (dog)	FHIT	NP_001135729.1
Danio rerio (zebrafish)	fhit	NP_957034.1
Magnaporthe oryzae (rice blast fungus)	MGG_01517	XP_003714490.1
Rattus norvegicus (Norway rat)	Fhit	NP_068542.1
Xenopus tropicalis (tropical clawed frog)	fhit	NP_001006883.1
Danio rerio (zebrafish)	LOC100331277	XP_002662597.1
Eremothecium gossypii	AGOS_AAL053C	NP_982489.2
Arabidopsis thaliana (thale cress)	FHIT	NP_200632.2
Bos taurus (cattle)	FHIT	NP_001035736.1
Mus musculus (house mouse)	Fhit	NP_034340.1
Oryza sativa (rice)	Os11g0120600	NP_001065609.2
Homo sapiens (human)	FHIT	NP_001159715.1
Gallus gallus (chicken)	FHIT	NP_001264731.1
Saccharomyces cerevisiae (baker's yeast)	HNT2	NP_010591.1
Kluyveromyces lactis	KLLA0C10142g	XP_452651.1
Schizosaccharomyces pombe (fission yeast)	aph1	NP_587836.1

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Corominas R, Yang X, Lin GN, Kang S, Shen Y, Ghamsari L, Broly M, Rodriguez M, Tam S, Trigg SA, Fan C, Yi S, Tasan M, Lemmens I, Kuang X, Zhao N, Malhotra D, Michaelson JJ, Vacic V, Calderwood MA, Roth FP, Tavernier J, Horvath S, Salehi-Ashtiani K, Korkin D, Sebat J, Hill DE, Hao T, Vidal M, Iakoucheva LM
Nature communications53650(2014 Apr)

The tumor suppressor Fhit acts as a repressor of beta-catenin transcriptional activity.
Weiske J, Albring KF, Huber O
Proceedings of the National Academy of Sciences of the United States of America104(51)20344-9(2007 Dec)

Association between germline variation in the FHIT gene and prostate cancer in Caucasians and African Americans.
Levin AM, Ray AM, Zuhlke KA, Douglas JA, Cooney KA
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology16(6)1294-7(2007 Jun)

Differential gene expression in nickel(II)-treated normal rat kidney cells.
Lee SH
Research communications in molecular pathology and pharmacology119(1-6)77-87(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nickel(II) treatment down-regulated Fhit expression.
Title: Differential gene expression in nickel(II)-treated normal rat kidney cells.

There is an overall association of Fhit expression with regulatory region methylation, and hypermethylation patterns did not vary with carcinogen.
Title: Hypermethylation patterns in the Fhit regulatory region are tissue specific.

These results suggest that Fhit alterations may play important roles in hepatocarcinogenesis due to choline deficiency in rats.
Title: Fhit gene alterations in hepatocarcinogenesis induced by a choline-deficient L-amino acid-defined diet in rats.

examination of alterations in N-nitrosodiethylamine-induced hepatocellular carcinoma
Title: Alterations of the Fhit gene in hepatocellular carcinomas induced by N-nitrosodiethylamine in rats.

Distribution of Fhit protein in rat tissues and its intracellular localization.
Title: Distribution of Fhit protein in rat tissues and its intracellular localization.

The following Fhit gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fhit cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05226	NM_021774.1 Latest version!	Rattus norvegicus fragile histidine triad diadenosine triphosphatase (Fhit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fhit ( NM_021774.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068542.1 Rattus norvegicus fragile histidine triad diadenosine triphosphatase (Fhit), mRNA.

Price & Availability↑

CloneID	ORa05226
Clone ID Related Accession (Same CDS sequence)	NM_021774.1
Accession Version	NM_021774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	bis(5'-adenosyl)-triphosphatase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF170064.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF170064.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCATTTA AATTCGGCCA ACATCTCATC AAGCCCTCTG TGGTTTTCCT CAAAACCGAA 
CTGTCCTTTG CCCTGGTGAA TAGGAAACCC GTTGTACCTG GCCATGTCCT CATGTGTCCA 
CTTAGGCCAG TGGAGCGCTT CCGTGACTTA CGTCCTGATG AAGTGGCCGA TTTGTTTCAA 
GTAACACAGA GAGTTGGGAC AGTGGTGGAG AAGCATTTCC AGGGGACCTC CATCACCTTC 
TCCATGCAAG ATGGTCCTGA AGCTGGACAG ACTGTGAAGC ATGTACATGT CCACATTCTT 
CCCAGGAAGT CAGGAGACTT CCGCAGGAAT GACAACATCT ATGATGAGCT TCAAAAACAT 
GACAGAGAAG AAGAGGACTC ACCAGCCTTT TGGAGATCTG AGGAGGAGAT GGCTGCAGAG 
GCGGAAGTTC TGAGGGCCTA CTTTCAGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068542.1
CDS	147..599
Translation	MSFKFGQHLIKPSVVFLKTELSFALVNRKPVVPGHVLMCPLRPVERFRDLRPDEVADLFQVTQRVGTVVEKHFQGTSITFSMQDGPEAGQTVKHVHVHILPRKSGDFRRNDNIYDELQKHDREEEDSPAFWRSEEEMAAEAEVLRAYFQA

Target ORF information:

RefSeq Version	NM_021774.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fragile histidine triad diadenosine triphosphatase (Fhit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021774.1

ORF Insert Sequence:

ATGTCATTTA AATTCGGCCA ACATCTCATC AAGCCCTCTG TGGTTTTCCT CAAAACCGAA 
CTGTCCTTTG CCCTGGTGAA TAGGAAACCC GTTGTACCTG GCCATGTCCT CATGTGTCCA 
CTTAGGCCAG TGGAGCGCTT CCGTGACTTA CGTCCTGATG AAGTGGCCGA TTTGTTTCAA 
GTAACACAGA GAGTTGGGAC AGTGGTGGAG AAGCATTTCC AGGGGACCTC CATCACCTTC 
TCCATGCAAG ATGGTCCTGA AGCTGGACAG ACTGTGAAGC ATGTACATGT CCACATTCTT 
CCCAGGAAGT CAGGAGACTT CCGCAGGAAT GACAACATCT ATGATGAGCT TCAAAAACAT 
GACAGAGAAG AAGAGGACTC ACCAGCCTTT TGGAGATCTG AGGAGGAGAT GGCTGCAGAG 
GCGGAAGTTC TGAGGGCCTA CTTTCAGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Nature communications53650(2014 Apr)
Corominas R,Yang X,Lin GN,Kang S,Shen Y,Ghamsari L,Broly M,Rodriguez M,Tam S,Trigg SA,Fan C,Yi S,Tasan M,Lemmens I,Kuang X,Zhao N,Malhotra D,Michaelson JJ,Vacic V,Calderwood MA,Roth FP,Tavernier J,Horvath S,Salehi-Ashtiani K,Korkin D,Sebat J,Hill DE,Hao T,Vidal M,Iakoucheva LM

The tumor suppressor Fhit acts as a repressor of beta-catenin transcriptional activity.
Proceedings of the National Academy of Sciences of the United States of America104(51)20344-9(2007 Dec)
Weiske J,Albring KF,Huber O

Association between germline variation in the FHIT gene and prostate cancer in Caucasians and African Americans.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology16(6)1294-7(2007 Jun)
Levin AM,Ray AM,Zuhlke KA,Douglas JA,Cooney KA

Differential gene expression in nickel(II)-treated normal rat kidney cells.
Research communications in molecular pathology and pharmacology119(1-6)77-87(2006)
Lee SH

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Fhit cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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