Gabarap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gabarap
Entrez Gene ID	58974
Full Name	GABA type A receptor-associated protein
General protein information	Preferred Names GABA type A receptor-associated protein Names gamma-aminobutyric acid receptor-associated protein GABA(A) receptor-associated protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24
Summary	putative microtubule binding protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172036.3	NP_742033.1	gamma-aminobutyric acid receptor-associated protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GABARAP	NP_009209.1
Rattus norvegicus (Norway rat)	Gabarap	NP_742033.1
	GABARAP	XP_001107079.1
Mus musculus (house mouse)	Gabarap	NP_062723.1
Danio rerio (zebrafish)	gabarapa	NP_001013278.1
Pan troglodytes (chimpanzee)	GABARAP	XP_003315392.1
Canis lupus familiaris (dog)	GABARAP	XP_536616.2
Bos taurus (cattle)	GABARAP	NP_001029220.1

Gene Ontology
Bibliography

Related articles in PubMed

Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs.
Szalai P, Hagen LK, S?tre F, Luhr M, Sponheim M, ?verbye A, Mills IG, Seglen PO, Engedal N
Experimental cell research333(1)21-38(2015 Apr)

TRIM proteins regulate autophagy and can target autophagic substrates by direct recognition.
Mandell MA, Jain A, Arko-Mensah J, Chauhan S, Kimura T, Dinkins C, Silvestri G, M?nch J, Kirchhoff F, Simonsen A, Wei Y, Levine B, Johansen T, Deretic V
Developmental cell30(4)394-409(2014 Aug)

Interaction of Bcl-2 with the autophagy-related GABAA receptor-associated protein (GABARAP): biophysical characterization and functional implications.
Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S, Mohrl?der J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergr?ber OH, Willbold D
The Journal of biological chemistry288(52)37204-15(2013 Dec)

Functional interaction between autophagy and ciliogenesis.
Pampliega O, Orhon I, Patel B, Sridhar S, D?az-Carretero A, Beau I, Codogno P, Satir BH, Satir P, Cuervo AM
Nature502(7470)194-200(2013 Oct)

Postfertilization autophagy of sperm organelles prevents paternal mitochondrial DNA transmission.
Al Rawi S, Louvet-Vall?e S, Djeddi A, Sachse M, Culetto E, Hajjar C, Boyd L, Legouis R, Galy V
Science (New York, N.Y.)334(6059)1144-7(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Macroautophagic sequestration does not require the LC3 subfamily but the GABARAP subfamily is essential.
Title: Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs.

These results support the regulatory role of Bcl-2 in autophagy and define GABARAP as a novel interaction partner involved in this intricate connection.
Title: Interaction of Bcl-2 with the autophagy-related GABAA receptor-associated protein (GABARAP): biophysical characterization and functional implications.

GABARAP and AT(1)R interact through discrete domains and this association regulates the cell-surface accumulation and, consequently, ligand-induced function of the receptor.
Title: C-terminal processing of GABARAP is not required for trafficking of the angiotensin II type 1A receptor.

Carboxyl-terminal processing of GABARAP is essential for the localization of GABARAP and its functions as a trafficking protein.
Title: C-terminal modification is required for GABARAP-mediated GABA(A) receptor trafficking.

Coexpression of GABARAP increases the steady-state expression of GABA-A receptors at the cell surface. Coexpression of GABARAP increases GABA currents by 2- to 3-fold.
Title: GABAA receptor-associated protein regulates GABAA receptor cell-surface number in Xenopus laevis oocytes.

The following Gabarap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gabarap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12232	NM_172036.3 Latest version!	Rattus norvegicus GABA type A receptor-associated protein (Gabarap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gabarap ( NM_172036.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742033.1 Rattus norvegicus GABA type A receptor-associated protein (Gabarap), mRNA.

Price & Availability↑

CloneID	ORa12232
Clone ID Related Accession (Same CDS sequence)	NM_172036.3
Accession Version	NM_172036.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	gamma-aminobutyric acid receptor-associated protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC058441.1. On Aug 30, 2012 this sequence version replaced NM_172036.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058441.1, FQ212169.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTCG TGTACAAAGA GGAGCATCCG TTCGAGAAGC GCCGCTCTGA GGGCGAGAAA 
ATCCGAAAGA AATACCCAGA CCGGGTCCCG GTGATAGTGG AAAAAGCTCC CAAAGCTCGG 
ATAGGGGACC TGGACAAAAA GAAATACTTG GTGCCTTCTG ATCTTACAGT TGGTCAATTC 
TACTTCTTGA TCCGGAAGCG AATTCATCTC CGTGCTGAAG ATGCCTTGTT TTTCTTTGTC 
AACAATGTCA TTCCACCCAC CAGTGCCACG ATGGGTCAGC TGTACCAGGA ACACCATGAA 
GAAGACTTCT TTCTATACAT TGCCTACAGT GATGAAAGCG TCTACGGTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742033.1
CDS	50..403
Translation	MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYGL

Target ORF information:

RefSeq Version	NM_172036.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GABA type A receptor-associated protein (Gabarap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172036.3

ORF Insert Sequence:

ATGAAGTTCG TGTACAAAGA GGAGCATCCG TTCGAGAAGC GCCGCTCTGA GGGCGAGAAA 
ATCCGAAAGA AATACCCAGA CCGGGTCCCG GTGATAGTGG AAAAAGCTCC CAAAGCTCGG 
ATAGGGGACC TGGACAAAAA GAAATACTTG GTGCCTTCTG ATCTTACAGT TGGTCAATTC 
TACTTCTTGA TCCGGAAGCG AATTCATCTC CGTGCTGAAG ATGCCTTGTT TTTCTTTGTC 
AACAATGTCA TTCCACCCAC CAGTGCCACG ATGGGTCAGC TGTACCAGGA ACACCATGAA 
GAAGACTTCT TTCTATACAT TGCCTACAGT GATGAAAGCG TCTACGGTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs.
Experimental cell research333(1)21-38(2015 Apr)
Szalai P,Hagen LK,S?tre F,Luhr M,Sponheim M,?verbye A,Mills IG,Seglen PO,Engedal N

TRIM proteins regulate autophagy and can target autophagic substrates by direct recognition.
Developmental cell30(4)394-409(2014 Aug)
Mandell MA,Jain A,Arko-Mensah J,Chauhan S,Kimura T,Dinkins C,Silvestri G,M?nch J,Kirchhoff F,Simonsen A,Wei Y,Levine B,Johansen T,Deretic V

Interaction of Bcl-2 with the autophagy-related GABAA receptor-associated protein (GABARAP): biophysical characterization and functional implications.
The Journal of biological chemistry288(52)37204-15(2013 Dec)
Ma P,Schwarten M,Schneider L,Boeske A,Henke N,Lisak D,Weber S,Mohrl?der J,Stoldt M,Strodel B,Methner A,Hoffmann S,Weiergr?ber OH,Willbold D

Functional interaction between autophagy and ciliogenesis.
Nature502(7470)194-200(2013 Oct)
Pampliega O,Orhon I,Patel B,Sridhar S,D?az-Carretero A,Beau I,Codogno P,Satir BH,Satir P,Cuervo AM

Postfertilization autophagy of sperm organelles prevents paternal mitochondrial DNA transmission.
Science (New York, N.Y.)334(6059)1144-7(2011 Nov)
Al Rawi S,Louvet-Vall?e S,Djeddi A,Sachse M,Culetto E,Hajjar C,Boyd L,Legouis R,Galy V

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Gabarap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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