Robo1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Robo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Robo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa05387 NM_022188.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa57708 XM_017598056.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57709 XM_017598057.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57710 XM_017598058.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa57711 XM_017598059.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa57712 XM_017598060.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa57713 XM_017598061.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa57714 XM_017598062.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa57715 XM_017598063.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa57716 XM_017598064.1
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ORa43464 XM_008768533.2
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
ORa43465 XM_008768534.2
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
ORa43467 XM_008768536.2
Latest version!
Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa05387
    Clone ID Related Accession (Same CDS sequence) NM_022188.1
    Accession Version NM_022188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF041082.1. On Jun 25, 2006 this sequence version replaced XM_001066378.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF041082.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATCTTCC TCTTCTGGTC ATGATATCTC TCCTCACTCT ATCAAAAAAG 
    CATCTCCTTC TGGCCCAGCT TATTCCAGAC CCTGAGGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAG CCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTCCC ACCGCGCATT GTTGAGCACC CTTCAGACCT GATCGTCTCT
    AAAGGGGAAC CCGCCACCCT CAACTGTAAA GCTGAAGGCC GCCCTACCCC GACTATAGAA
    TGGTACAAGG GCGGCGAAAG AGTGGAAACG GACAAAGACG ACCCTCGCTC ACACCGAATG
    CTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGCATAGTGC ATGGACGGAA GAGTAGGCCT
    GACGAAGGGG TCTACATCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGCCACAAT
    GCATCGCTGG AGGTAGCTAT ACTACGGGAT GACTTCAGAC AAAACCCTTC AGATGTTATG
    GTGGCGGTGG GCGAGCCTGC AGTAATGGAA TGCCAGCCTC CTCGGGGCCA CCCAGAGCCC
    ACCATCTCCT GGAAAAAGGA TGGCTCACCC CTGGATGATA AAGACGAGCG AATCACTATC
    CGAGGCGGGA AGCTCATGAT CACGTACACC CGTAAAAGCG ATGCTGGCAA GTATGTTTGT
    GTTGGTACCA ACATGGTAGG GGAGCGGGAG AGCAAAGTCG CGGACGTGAC TGTCTTAGAG
    AGACCGTCCT TTGTAAAGAG ACCCAGTAAT TTGGCAGTGA CTGTGGATGA CAGTGCTGAG
    TTTAAGTGTG AGGCCCGAGG AGACCCCGTG CCTACGTTCG GGTGGAGGAA AGATGACGGA
    GAGCTGCCCA AATCCAGATA TGAGATCCGA GATGATCATA CTTTGAAAAT TCGGAAGGTG
    ACAGCTGGCG ACATGGGATC ATACACTTGT GTTGCAGAAA ATATGGTTGG GAAAGCCGAA
    GCATCAGCCA CTTTGACAGT TCAAGAGCCA CCACATTTTG TCGTGAAGCC TCGAGACCAG
    GTGGTTGCCT TGGGGCGAAC TGTGACTTTT CAGTGTGAAG CAACTGGAAA TCCTCAACCA
    GCTATCTTTT GGCGGAGAGA GGGGAGTCAG AATCTGCTTT TCTCATACCA GCCTCCACAG
    TCCTCTAGCC GGTTTTCAGT TTCCCAGACG GGGGACCTCA CTGTTACGAA CGTCCAGAGG
    TCTGATGTCG GTTACTATAT CTGTCAGACT TTGAACGTGG CTGGAAGCAT CATCACAAAG
    GCATATTTGG AAGTTACTGA TGTGATTGCG GACCGGCCTC CCCCAGTCAT CCGACAAGGT
    CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAACTC TCAGCTGTGT GGCCACAGGC
    AGTCCCGTGC CCACAATTCT GTGGAGGAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT
    CGGATCAAAC AGCTGGAGAG CGGGGTCCTG CAGATCCGCT ACGCTAAGCT GGGTGACACC
    GGTCGGTACA CCTGCACTGC TTCGACCCCC AGTGGTGAGG CCACGTGGAG CGCTTACATC
    GAAGTCCAGG AATTTGGAGT TCCAGTTCAA CCTCCAAGAC CCACCGACCC CAATTTAATC
    CCCAGTGCGC CTTCAAAGCC TGAAGTCACA GATGTGAGCA AAAACACAGT CACTTTATTG
    TGGCAGCCTA ACCTGAACTC AGGAGCAACC CCAACGTCTT ATATTATAGA AGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAGACCGTG GCAGAAAATG TGAAAACAGA AACATTCGCC
    ATCAAAGGGC TCAAACCTAA TGCGATCTAC CTTTTCCTGG TGAGGGCAGC AAATGCCTAT
    GGGATTAGCG ATCCAAGCCA AATATCCGAT CCAGTGAAAA CACAAGATGT CCCACCAACC
    ACCCAAGGGG TGGACCACAA GCAGGTCCAG AGAGAACTGG GCAATGTTGT CTTGCATCTC
    CACAACCCTA CCATCCTTTC TTCCTCTTCT GTCGAAGTGC ACTGGACGGT GGACCAACAA
    TCTCAATATA TTCAAGGTTA TAAAATTCTC TACCGACCAT CTGGAGCCAG CCATGGCGAA
    TCGGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ATAGTGTGGT GATCCCCGAT
    CTGAGAAAAG GCGTCAACTA CGAGATTAAG GCCCGCCCAT TTTTTAATGA GTTCCAAGGA
    GCAGATAGTG AAATCAAATT TGCCAAAACC TTAGAAGAAC GACCAAGTGC CCCTCCCCGA
    AGTGTCACTG TATCCAAGAA TGATGGAAAT GGGACAGCAA TTCTGGTCAC TTGGCAACCG
    CCTCCTGAAG ACACACAGAA TGGAATGGTC CAAGAATATA AGGTTTGGTG TCTCGGGAAT
    GAGACTAGGT ATCACATAAA CAAGACAGTA GATGGCTCTA CCTTTTCGGT GGTCATTCCC
    TTTCTTGTTC CTGGAATCCG CTACAGTGTA GAAGTGGCTG CAAGCACTGG GGCTGGCCCT
    GGGGTGAAGA GTGAGCCCCA GTTCATCCAG TTGGATTCCC ATGGAAACCC CGTGTCTCCC
    GAGGACCAAG TGAGCCTGGC TCAGCAGATC TCCGACGTGG TGAAGCAGCC AGCCTTCATT
    GCGGGCATCG GGGCCGCCTG TTGGATCATT CTCATGGTCT TCAGTATCTG GCTTTACCGA
    CACCGCAAGA AGAGAAACGG GCTCAGCAGC ACGTACGCGG GGATCCGGAA AGTCCCGTCC
    TTTACCTTCA CACCAACAGT AACTTATCAA AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG
    AGGCCGGGAC TCCTCAACAT CAGTGAACCT GCCACACAGC CATGGCTGGC GGACACGTGG
    CCCAACACAG GCAACAGTCA CAATGACTGC TCCATCAACT GCTGCACAGC CAGCAACGGC
    AACAGCGACA GCAACCTGAC CACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC
    AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT
    GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT
    GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCCTACG CCACCACGCA GCTCATCCAG
    GCAAATCTCA TCAACAATAT GAATAACGGC GGCGGGGACT CCAGCGAGAA ACACTGGAAG
    CCTCCGGGGC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAGTTGAACA AAGATTATCG GGCAAATGAC ACAATCCTTC CAACCATCCC GTACAACCAT
    TCNTATGACC AGAATACAGG AGGATCCTAC AACAGCTCAG ATCGGGGCAG TAGTACATCT
    GGGAGTCAAG GCCACAAGAA AGGGGCACGG ACACCAAAGG CACCCAAGCA GGGTGGCATG
    AACTGGGCAG ACTTGCTGCC CCCTCCCCCA GCGCATCCTC CCCCACACAG CAACAGCGAG
    GAATACAGCA TGTCTGTAGA TGAAAGCTAT GATCAAGAAA TGCCGTGCCC TGTTCCGCCT
    GCACGGATGT ATTTGCAACA GGATGAATTA GAAGAAGAGG AAGCTGAAAG AGGCCCCACG
    CCCCCTGTCC GGGGGGCAGC TTCGTCCCCA GCTGCTGTGT CCTACAGCCA TCAGTCCACG
    GCCACTCTCA CCCCGTCTCC CCAGGAAGAG CTCCAGCCTA TGTTACAGGA TTGTCCGGAG
    GACCTCGGCC ACATGCCCCA CCCACCAGAC AGGAGGCGGC AGCCGGTGAG TCCTCCTCCA
    CCACCACGGC CAATTTCCCC ACCACATACT TACGGCTACA TTTCAGGACC CCTGGTCTCA
    GACATGGATA CGGATGCCCC AGAAGAGGAG GAAGACGAAG CTGACATGGA AGTTGCTAAG
    ATGCAGACCC GAAGGCTGTT GTTACGTGGG CTTGAACAGA CCCCAGCATC TAGTGTTGGG
    GACCTCGAGA GCTCGGTCAC TGGCTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAAGAG
    GACAACATTT CCAGTGGGCG CTCCAGTGTC AGTTCATCGG ATGGTTCCTT TTTCACTGAC
    GCGGATTTTG CCCAGGCAGT TGCAGCAGCC GCCGAGTATG CGGGCCTGAA GGTGGCTCGC
    CGCCAGATGC AGGATGCTGC TGGCCGCCGA CACTTCCATG CCTCCCAGTG CCCTAGGCCC
    ACGAGTCCTG TGTCTACAGA CAGCAACATG AGTGCTGCTG TAATCCAGAA AGCCAGACCC
    ACCAAGAAAC AGAAGCACCA GCCAGGACAT CTGCGCAGAG AGGCCTACAC AGATGATCTT
    CCACCACCTC CAGTGCCACC GCCTGCTATA AAATCGCCCA GTGTCCAGTC CAAGGCACAG
    CTGGAGGCAC GGCCTATAAT GGGGCCAAAG CTCGCTTCTA TAGAAGCGAG AGCAGACAGA
    TCATCAGACA GAAAAGGCGG CAGTTACAAG GGGAGAGAAG CTCTGGACGG AAGACAAGTC
    ACTGACCTGC GAACAAGCCC AGGTGACCCC AGAGAAGCAC AGGAGCAGCC AAACGAAGGG
    AAAGCACGAG GGACGAAGAC AGCAAAGCGA GACCTTCCAC CAGCGAAGAC ACACCTCATC
    CCAGAGGATA TCCTTCCGTA CTGTAGGCCT ACTTTCCCAA CGTCAAACAA CCCTAGGGAT
    CCCAGTTCTT CAAGCTCAAT GTCATCAAGA GGCTCAGGAA GCCGACAGAG AGAGCAAGCA
    AATGTGGGCC GAAGAAATAT GGCAGAAATG CAAGTACTTG GGGGATTTGA AAGAGGAGAT
    GAGAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_071524.1
    CDS1..4956
    Translation

    Target ORF information:

    RefSeq Version NM_022188.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATCTTCC TCTTCTGGTC ATGATATCTC TCCTCACTCT ATCAAAAAAG 
    CATCTCCTTC TGGCCCAGCT TATTCCAGAC CCTGAGGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAG CCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTCCC ACCGCGCATT GTTGAGCACC CTTCAGACCT GATCGTCTCT
    AAAGGGGAAC CCGCCACCCT CAACTGTAAA GCTGAAGGCC GCCCTACCCC GACTATAGAA
    TGGTACAAGG GCGGCGAAAG AGTGGAAACG GACAAAGACG ACCCTCGCTC ACACCGAATG
    CTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGCATAGTGC ATGGACGGAA GAGTAGGCCT
    GACGAAGGGG TCTACATCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGCCACAAT
    GCATCGCTGG AGGTAGCTAT ACTACGGGAT GACTTCAGAC AAAACCCTTC AGATGTTATG
    GTGGCGGTGG GCGAGCCTGC AGTAATGGAA TGCCAGCCTC CTCGGGGCCA CCCAGAGCCC
    ACCATCTCCT GGAAAAAGGA TGGCTCACCC CTGGATGATA AAGACGAGCG AATCACTATC
    CGAGGCGGGA AGCTCATGAT CACGTACACC CGTAAAAGCG ATGCTGGCAA GTATGTTTGT
    GTTGGTACCA ACATGGTAGG GGAGCGGGAG AGCAAAGTCG CGGACGTGAC TGTCTTAGAG
    AGACCGTCCT TTGTAAAGAG ACCCAGTAAT TTGGCAGTGA CTGTGGATGA CAGTGCTGAG
    TTTAAGTGTG AGGCCCGAGG AGACCCCGTG CCTACGTTCG GGTGGAGGAA AGATGACGGA
    GAGCTGCCCA AATCCAGATA TGAGATCCGA GATGATCATA CTTTGAAAAT TCGGAAGGTG
    ACAGCTGGCG ACATGGGATC ATACACTTGT GTTGCAGAAA ATATGGTTGG GAAAGCCGAA
    GCATCAGCCA CTTTGACAGT TCAAGAGCCA CCACATTTTG TCGTGAAGCC TCGAGACCAG
    GTGGTTGCCT TGGGGCGAAC TGTGACTTTT CAGTGTGAAG CAACTGGAAA TCCTCAACCA
    GCTATCTTTT GGCGGAGAGA GGGGAGTCAG AATCTGCTTT TCTCATACCA GCCTCCACAG
    TCCTCTAGCC GGTTTTCAGT TTCCCAGACG GGGGACCTCA CTGTTACGAA CGTCCAGAGG
    TCTGATGTCG GTTACTATAT CTGTCAGACT TTGAACGTGG CTGGAAGCAT CATCACAAAG
    GCATATTTGG AAGTTACTGA TGTGATTGCG GACCGGCCTC CCCCAGTCAT CCGACAAGGT
    CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAACTC TCAGCTGTGT GGCCACAGGC
    AGTCCCGTGC CCACAATTCT GTGGAGGAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT
    CGGATCAAAC AGCTGGAGAG CGGGGTCCTG CAGATCCGCT ACGCTAAGCT GGGTGACACC
    GGTCGGTACA CCTGCACTGC TTCGACCCCC AGTGGTGAGG CCACGTGGAG CGCTTACATC
    GAAGTCCAGG AATTTGGAGT TCCAGTTCAA CCTCCAAGAC CCACCGACCC CAATTTAATC
    CCCAGTGCGC CTTCAAAGCC TGAAGTCACA GATGTGAGCA AAAACACAGT CACTTTATTG
    TGGCAGCCTA ACCTGAACTC AGGAGCAACC CCAACGTCTT ATATTATAGA AGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAGACCGTG GCAGAAAATG TGAAAACAGA AACATTCGCC
    ATCAAAGGGC TCAAACCTAA TGCGATCTAC CTTTTCCTGG TGAGGGCAGC AAATGCCTAT
    GGGATTAGCG ATCCAAGCCA AATATCCGAT CCAGTGAAAA CACAAGATGT CCCACCAACC
    ACCCAAGGGG TGGACCACAA GCAGGTCCAG AGAGAACTGG GCAATGTTGT CTTGCATCTC
    CACAACCCTA CCATCCTTTC TTCCTCTTCT GTCGAAGTGC ACTGGACGGT GGACCAACAA
    TCTCAATATA TTCAAGGTTA TAAAATTCTC TACCGACCAT CTGGAGCCAG CCATGGCGAA
    TCGGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ATAGTGTGGT GATCCCCGAT
    CTGAGAAAAG GCGTCAACTA CGAGATTAAG GCCCGCCCAT TTTTTAATGA GTTCCAAGGA
    GCAGATAGTG AAATCAAATT TGCCAAAACC TTAGAAGAAC GACCAAGTGC CCCTCCCCGA
    AGTGTCACTG TATCCAAGAA TGATGGAAAT GGGACAGCAA TTCTGGTCAC TTGGCAACCG
    CCTCCTGAAG ACACACAGAA TGGAATGGTC CAAGAATATA AGGTTTGGTG TCTCGGGAAT
    GAGACTAGGT ATCACATAAA CAAGACAGTA GATGGCTCTA CCTTTTCGGT GGTCATTCCC
    TTTCTTGTTC CTGGAATCCG CTACAGTGTA GAAGTGGCTG CAAGCACTGG GGCTGGCCCT
    GGGGTGAAGA GTGAGCCCCA GTTCATCCAG TTGGATTCCC ATGGAAACCC CGTGTCTCCC
    GAGGACCAAG TGAGCCTGGC TCAGCAGATC TCCGACGTGG TGAAGCAGCC AGCCTTCATT
    GCGGGCATCG GGGCCGCCTG TTGGATCATT CTCATGGTCT TCAGTATCTG GCTTTACCGA
    CACCGCAAGA AGAGAAACGG GCTCAGCAGC ACGTACGCGG GGATCCGGAA AGTCCCGTCC
    TTTACCTTCA CACCAACAGT AACTTATCAA AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG
    AGGCCGGGAC TCCTCAACAT CAGTGAACCT GCCACACAGC CATGGCTGGC GGACACGTGG
    CCCAACACAG GCAACAGTCA CAATGACTGC TCCATCAACT GCTGCACAGC CAGCAACGGC
    AACAGCGACA GCAACCTGAC CACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC
    AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT
    GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAT
    GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCCTACG CCACCACGCA GCTCATCCAG
    GCAAATCTCA TCAACAATAT GAATAACGGC GGCGGGGACT CCAGCGAGAA ACACTGGAAG
    CCTCCGGGGC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAGTTGAACA AAGATTATCG GGCAAATGAC ACAATCCTTC CAACCATCCC GTACAACCAT
    TCNTATGACC AGAATACAGG AGGATCCTAC AACAGCTCAG ATCGGGGCAG TAGTACATCT
    GGGAGTCAAG GCCACAAGAA AGGGGCACGG ACACCAAAGG CACCCAAGCA GGGTGGCATG
    AACTGGGCAG ACTTGCTGCC CCCTCCCCCA GCGCATCCTC CCCCACACAG CAACAGCGAG
    GAATACAGCA TGTCTGTAGA TGAAAGCTAT GATCAAGAAA TGCCGTGCCC TGTTCCGCCT
    GCACGGATGT ATTTGCAACA GGATGAATTA GAAGAAGAGG AAGCTGAAAG AGGCCCCACG
    CCCCCTGTCC GGGGGGCAGC TTCGTCCCCA GCTGCTGTGT CCTACAGCCA TCAGTCCACG
    GCCACTCTCA CCCCGTCTCC CCAGGAAGAG CTCCAGCCTA TGTTACAGGA TTGTCCGGAG
    GACCTCGGCC ACATGCCCCA CCCACCAGAC AGGAGGCGGC AGCCGGTGAG TCCTCCTCCA
    CCACCACGGC CAATTTCCCC ACCACATACT TACGGCTACA TTTCAGGACC CCTGGTCTCA
    GACATGGATA CGGATGCCCC AGAAGAGGAG GAAGACGAAG CTGACATGGA AGTTGCTAAG
    ATGCAGACCC GAAGGCTGTT GTTACGTGGG CTTGAACAGA CCCCAGCATC TAGTGTTGGG
    GACCTCGAGA GCTCGGTCAC TGGCTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAAGAG
    GACAACATTT CCAGTGGGCG CTCCAGTGTC AGTTCATCGG ATGGTTCCTT TTTCACTGAC
    GCGGATTTTG CCCAGGCAGT TGCAGCAGCC GCCGAGTATG CGGGCCTGAA GGTGGCTCGC
    CGCCAGATGC AGGATGCTGC TGGCCGCCGA CACTTCCATG CCTCCCAGTG CCCTAGGCCC
    ACGAGTCCTG TGTCTACAGA CAGCAACATG AGTGCTGCTG TAATCCAGAA AGCCAGACCC
    ACCAAGAAAC AGAAGCACCA GCCAGGACAT CTGCGCAGAG AGGCCTACAC AGATGATCTT
    CCACCACCTC CAGTGCCACC GCCTGCTATA AAATCGCCCA GTGTCCAGTC CAAGGCACAG
    CTGGAGGCAC GGCCTATAAT GGGGCCAAAG CTCGCTTCTA TAGAAGCGAG AGCAGACAGA
    TCATCAGACA GAAAAGGCGG CAGTTACAAG GGGAGAGAAG CTCTGGACGG AAGACAAGTC
    ACTGACCTGC GAACAAGCCC AGGTGACCCC AGAGAAGCAC AGGAGCAGCC AAACGAAGGG
    AAAGCACGAG GGACGAAGAC AGCAAAGCGA GACCTTCCAC CAGCGAAGAC ACACCTCATC
    CCAGAGGATA TCCTTCCGTA CTGTAGGCCT ACTTTCCCAA CGTCAAACAA CCCTAGGGAT
    CCCAGTTCTT CAAGCTCAAT GTCATCAAGA GGCTCAGGAA GCCGACAGAG AGAGCAAGCA
    AATGTGGGCC GAAGAAATAT GGCAGAAATG CAAGTACTTG GGGGATTTGA AAGAGGAGAT
    GAGAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57708
    Clone ID Related Accession (Same CDS sequence) XM_017598056.1
    Accession Version XM_017598056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCACC ACATTTTGTC GTGAAGCCTC GAGACCAGGT GGTTGCCTTG
    GGGCGAACTG TGACTTTTCA GTGTGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    CGGAGAGAGG GGAGTCAGAA TCTGCTTTTC TCATACCAGC CTCCACAGTC CTCTAGCCGG
    TTTTCAGTTT CCCAGACGGG GGACCTCACT GTTACGAACG TCCAGAGGTC TGATGTCGGT
    TACTATATCT GTCAGACTTT GAACGTGGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA
    GTTACTGATG TGATTGCGGA CCGGCCTCCC CCAGTCATCC GACAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGTAC TTTAACTCTC AGCTGTGTGG CCACAGGCAG TCCCGTGCCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGCG GGGTCCTGCA GATCCGCTAC GCTAAGCTGG GTGACACCGG TCGGTACACC
    TGCACTGCTT CGACCCCCAG TGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGAGTTC CAGTTCAACC TCCAAGACCC ACCGACCCCA ATTTAATCCC CAGTGCGCCT
    TCAAAGCCTG AAGTCACAGA TGTGAGCAAA AACACAGTCA CTTTATTGTG GCAGCCTAAC
    CTGAACTCAG GAGCAACCCC AACGTCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACCGTGGC AGAAAATGTG AAAACAGAAA CATTCGCCAT CAAAGGGCTC
    AAACCTAATG CGATCTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG GATTAGCGAT
    CCAAGCCAAA TATCCGATCC AGTGAAAACA CAAGATGTCC CACCAACCAC CCAAGGGGTG
    GACCACAAGC AGGTCCAGAG AGAACTGGGC AATGTTGTCT TGCATCTCCA CAACCCTACC
    ATCCTTTCTT CCTCTTCTGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGTTATA AAATTCTCTA CCGACCATCT GGAGCCAGCC ATGGCGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACCCC AACCAAAAAT AGTGTGGTGA TCCCCGATCT GAGAAAAGGC
    GTCAACTACG AGATTAAGGC CCGCCCATTT TTTAATGAGT TCCAAGGAGC AGATAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTCACTGTA
    TCCAAGAATG ATGGAAATGG GACAGCAATT CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAGAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGGAATGA GACTAGGTAT
    CACATAAACA AGACAGTAGA TGGCTCTACC TTTTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGCT ACAGTGTAGA AGTGGCTGCA AGCACTGGGG CTGGCCCTGG GGTGAAGAGT
    GAACCCCAGT TCATCCAGTT AGATTCCCAT GGAAACCCCG TGTCTCCCGA GGACCAAGTG
    AGCCTGGCTC AGCAGATCTC CGACGTGGTG AAGCAGCCAG CCTTCATTGC GGGCATCGGG
    GCCGCCTGTT GGATCATTCT CATGGTCTTC AGTATCTGGC TTTACCGACA CCGCAAGAAG
    AGAAACGGGC TCAGCAGCAC GTACGCGGGG ATCCGGAAAG TCCCGTCCTT TACCTTCACA
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATCA GTGAACCTGC CACACAGCCA TGGCTGGCGG ACACGTGGCC CAACACAGGC
    AACAGTCACA ATGACTGCTC CATCAACTGC TGCACAGCCA GCAACGGCAA CAGCGACAGC
    AACCTGACCA CCTACAGTCG CCCAGCTGAT TGTATAGCAA ATTATAACAA CCAACTGGAT
    AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT
    AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGACGG GCGTTTTGTC
    AATCCATCAG GGCAGCCCAC TCCCTACGCC ACCACGCAGC TCATCCAGGC AAATCTCAGC
    AACAATATGA ATAACGGCGG CGGGGACTCC AGCGAGAAAC ACTGGAAGCC TCCGGGGCAG
    CAGAAACAGG AAGTGGCACC AATTCAGTAT AACATCATGG AGCAAAACAA GCTGAACAAA
    GATTATCGGG CAAATGACAC AATCCCTCCA ACCATCCCGT ACAACCAATC CTATGACCAG
    AATACAGGAG GATCTTACAA CAGCTCAGAT CGGGGCAGTA GTACATCTGG GAGTCAAGGC
    CACAAGAAAG GGGCACGGAC ACCAAAGGCA CCCAAGCAGG GTGGCATGAA CTGGGCAGAC
    TTGCTGCCCC CTCCCCCAGC GCATCCTCCC CCACACAGCA ACAGCGAGGA ATACAGCATG
    TCTGTAGATG AAAGCTATGA TCAAGAAATG CCGTGCCCTG TTCCGCCTGC ACGGATGTAT
    TTGCAACAGG ATGAATTAGA AGAAGAGGAA GCTGAAAGAG GCCCCACGCC CCCTGTCCGG
    GGGGCAGCTT CGTCCCCAGC TGCTGTGTCC TACAGCCATC AGTCCACGGC CACTCTCACC
    CCGTCTCCCC AGGAAGAGCT CCAGCCTATG TTACAGGATT GTCCGGAGGA CCTCGGCCAC
    ATGCCCCACC CACCAGACAG GAGGCGGCAG CCGGTGAGTC CTCCTCCACC ACCACGGCCA
    ATTTCCCCAC CACATACTTA CGGCTACATT TCAGGACCCC TGGTCTCAGA CATGGATACG
    GATGCCCCAG AAGAGGAGGA AGACGAAGCT GACATGGAAG TTGCTAAGAT GCAGACCCGA
    AGGCTGTTGT TACGTGGGCT TGAACAGACC CCAGCATCTA GTGTTGGGGA CCTCGAGAGC
    TCGGTCACTG GCTCCATGAT CAATGGCTGG GGCTCAGCCT CAGAAGAGGA CAACATTTCC
    AGTGGGCGCT CCAGTGTCAG TTCATCGGAT GGTTCCTTTT TCACTGACGC TGATTTTGCC
    CAGGCAGTTG CCGCAGCCGC CGAGTATGCG GGCCTGAAGG TGGCTCGCCG CCAGATGCAG
    GATGCTGCTG GCCGCCGACA CTTCCATGCC TCCCAGTGCC CTAGGCCCAC GAGTCCTGTG
    TCTACAGACA GCAACATGAG TGCTGCTGTA ATCCAGAAAG CCAGACCCAC CAAGAAACAG
    AAGCACCAGC CAGGACATCT GCGCAGAGAG GCCTACACAG ATGATCTTCC ACCCCCTCCA
    GTGCCACCAC CTGCTATAAA ATCGCCCAGT GTCCAGTCCA AGGCACAGCT GGAGGCACGG
    CCTATAATGG GGCCAAAGCT CGCTTCTATA GAAGCGAGAG CAGACAGATC ATCAGACAGA
    AAAGGCGGCA GTTACAAGGG GAGAGAAGCT CTGGACGGAA GACAAGTCAC TGACCTGCGA
    ACAAGCCCAG GTGACCCCAG AGAAGCACAG GAGCAGCCAA ACGAAGGGAA AGCACGAGGG
    ACGAAGACAG CAAAGCGAGA CCTTCCACCA GCGAAGACAC ACCTCATCCC AGAGGATATC
    CTTCCGTACT GTAGGCCTAC TTTCCCAACG TCAAACAACC CTAGGGATCC CAGTTCTTCA
    AGCTCAATGT CATCAAGAGG CTCAGGAAGC CGACAGAGAG AGCAAGCAAA TGTGGGCCGA
    AGAAATATGG CAGAAATGCA AGTACTTGGG GGATTTGAAA GAGGAGATGA GAATAATGAA
    GAATTAGAGG AAACTGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453545.1
    CDS241..5304
    Translation

    Target ORF information:

    RefSeq Version XM_017598056.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCACC ACATTTTGTC GTGAAGCCTC GAGACCAGGT GGTTGCCTTG
    GGGCGAACTG TGACTTTTCA GTGTGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    CGGAGAGAGG GGAGTCAGAA TCTGCTTTTC TCATACCAGC CTCCACAGTC CTCTAGCCGG
    TTTTCAGTTT CCCAGACGGG GGACCTCACT GTTACGAACG TCCAGAGGTC TGATGTCGGT
    TACTATATCT GTCAGACTTT GAACGTGGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA
    GTTACTGATG TGATTGCGGA CCGGCCTCCC CCAGTCATCC GACAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGTAC TTTAACTCTC AGCTGTGTGG CCACAGGCAG TCCCGTGCCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGCG GGGTCCTGCA GATCCGCTAC GCTAAGCTGG GTGACACCGG TCGGTACACC
    TGCACTGCTT CGACCCCCAG TGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGAGTTC CAGTTCAACC TCCAAGACCC ACCGACCCCA ATTTAATCCC CAGTGCGCCT
    TCAAAGCCTG AAGTCACAGA TGTGAGCAAA AACACAGTCA CTTTATTGTG GCAGCCTAAC
    CTGAACTCAG GAGCAACCCC AACGTCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACCGTGGC AGAAAATGTG AAAACAGAAA CATTCGCCAT CAAAGGGCTC
    AAACCTAATG CGATCTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG GATTAGCGAT
    CCAAGCCAAA TATCCGATCC AGTGAAAACA CAAGATGTCC CACCAACCAC CCAAGGGGTG
    GACCACAAGC AGGTCCAGAG AGAACTGGGC AATGTTGTCT TGCATCTCCA CAACCCTACC
    ATCCTTTCTT CCTCTTCTGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGTTATA AAATTCTCTA CCGACCATCT GGAGCCAGCC ATGGCGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACCCC AACCAAAAAT AGTGTGGTGA TCCCCGATCT GAGAAAAGGC
    GTCAACTACG AGATTAAGGC CCGCCCATTT TTTAATGAGT TCCAAGGAGC AGATAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTCACTGTA
    TCCAAGAATG ATGGAAATGG GACAGCAATT CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAGAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGGAATGA GACTAGGTAT
    CACATAAACA AGACAGTAGA TGGCTCTACC TTTTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGCT ACAGTGTAGA AGTGGCTGCA AGCACTGGGG CTGGCCCTGG GGTGAAGAGT
    GAACCCCAGT TCATCCAGTT AGATTCCCAT GGAAACCCCG TGTCTCCCGA GGACCAAGTG
    AGCCTGGCTC AGCAGATCTC CGACGTGGTG AAGCAGCCAG CCTTCATTGC GGGCATCGGG
    GCCGCCTGTT GGATCATTCT CATGGTCTTC AGTATCTGGC TTTACCGACA CCGCAAGAAG
    AGAAACGGGC TCAGCAGCAC GTACGCGGGG ATCCGGAAAG TCCCGTCCTT TACCTTCACA
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATCA GTGAACCTGC CACACAGCCA TGGCTGGCGG ACACGTGGCC CAACACAGGC
    AACAGTCACA ATGACTGCTC CATCAACTGC TGCACAGCCA GCAACGGCAA CAGCGACAGC
    AACCTGACCA CCTACAGTCG CCCAGCTGAT TGTATAGCAA ATTATAACAA CCAACTGGAT
    AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT
    AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGACGG GCGTTTTGTC
    AATCCATCAG GGCAGCCCAC TCCCTACGCC ACCACGCAGC TCATCCAGGC AAATCTCAGC
    AACAATATGA ATAACGGCGG CGGGGACTCC AGCGAGAAAC ACTGGAAGCC TCCGGGGCAG
    CAGAAACAGG AAGTGGCACC AATTCAGTAT AACATCATGG AGCAAAACAA GCTGAACAAA
    GATTATCGGG CAAATGACAC AATCCCTCCA ACCATCCCGT ACAACCAATC CTATGACCAG
    AATACAGGAG GATCTTACAA CAGCTCAGAT CGGGGCAGTA GTACATCTGG GAGTCAAGGC
    CACAAGAAAG GGGCACGGAC ACCAAAGGCA CCCAAGCAGG GTGGCATGAA CTGGGCAGAC
    TTGCTGCCCC CTCCCCCAGC GCATCCTCCC CCACACAGCA ACAGCGAGGA ATACAGCATG
    TCTGTAGATG AAAGCTATGA TCAAGAAATG CCGTGCCCTG TTCCGCCTGC ACGGATGTAT
    TTGCAACAGG ATGAATTAGA AGAAGAGGAA GCTGAAAGAG GCCCCACGCC CCCTGTCCGG
    GGGGCAGCTT CGTCCCCAGC TGCTGTGTCC TACAGCCATC AGTCCACGGC CACTCTCACC
    CCGTCTCCCC AGGAAGAGCT CCAGCCTATG TTACAGGATT GTCCGGAGGA CCTCGGCCAC
    ATGCCCCACC CACCAGACAG GAGGCGGCAG CCGGTGAGTC CTCCTCCACC ACCACGGCCA
    ATTTCCCCAC CACATACTTA CGGCTACATT TCAGGACCCC TGGTCTCAGA CATGGATACG
    GATGCCCCAG AAGAGGAGGA AGACGAAGCT GACATGGAAG TTGCTAAGAT GCAGACCCGA
    AGGCTGTTGT TACGTGGGCT TGAACAGACC CCAGCATCTA GTGTTGGGGA CCTCGAGAGC
    TCGGTCACTG GCTCCATGAT CAATGGCTGG GGCTCAGCCT CAGAAGAGGA CAACATTTCC
    AGTGGGCGCT CCAGTGTCAG TTCATCGGAT GGTTCCTTTT TCACTGACGC TGATTTTGCC
    CAGGCAGTTG CCGCAGCCGC CGAGTATGCG GGCCTGAAGG TGGCTCGCCG CCAGATGCAG
    GATGCTGCTG GCCGCCGACA CTTCCATGCC TCCCAGTGCC CTAGGCCCAC GAGTCCTGTG
    TCTACAGACA GCAACATGAG TGCTGCTGTA ATCCAGAAAG CCAGACCCAC CAAGAAACAG
    AAGCACCAGC CAGGACATCT GCGCAGAGAG GCCTACACAG ATGATCTTCC ACCCCCTCCA
    GTGCCACCAC CTGCTATAAA ATCGCCCAGT GTCCAGTCCA AGGCACAGCT GGAGGCACGG
    CCTATAATGG GGCCAAAGCT CGCTTCTATA GAAGCGAGAG CAGACAGATC ATCAGACAGA
    AAAGGCGGCA GTTACAAGGG GAGAGAAGCT CTGGACGGAA GACAAGTCAC TGACCTGCGA
    ACAAGCCCAG GTGACCCCAG AGAAGCACAG GAGCAGCCAA ACGAAGGGAA AGCACGAGGG
    ACGAAGACAG CAAAGCGAGA CCTTCCACCA GCGAAGACAC ACCTCATCCC AGAGGATATC
    CTTCCGTACT GTAGGCCTAC TTTCCCAACG TCAAACAACC CTAGGGATCC CAGTTCTTCA
    AGCTCAATGT CATCAAGAGG CTCAGGAAGC CGACAGAGAG AGCAAGCAAA TGTGGGCCGA
    AGAAATATGG CAGAAATGCA AGTACTTGGG GGATTTGAAA GAGGAGATGA GAATAATGAA
    GAATTAGAGG AAACTGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57709
    Clone ID Related Accession (Same CDS sequence) XM_017598057.1
    Accession Version XM_017598057.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGAGCCAC CACATTTTGT CGTGAAGCCT CGAGACCAGG TGGTTGCCTT GGGGCGAACT
    GTGACTTTTC AGTGTGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GCGGAGAGAG
    GGGAGTCAGA ATCTGCTTTT CTCATACCAG CCTCCACAGT CCTCTAGCCG GTTTTCAGTT
    TCCCAGACGG GGGACCTCAC TGTTACGAAC GTCCAGAGGT CTGATGTCGG TTACTATATC
    TGTCAGACTT TGAACGTGGC TGGAAGCATC ATCACAAAGG CATATTTGGA AGTTACTGAT
    GTGATTGCGG ACCGGCCTCC CCCAGTCATC CGACAAGGTC CTGTGAATCA GACTGTAGCA
    GTGGATGGTA CTTTAACTCT CAGCTGTGTG GCCACAGGCA GTCCCGTGCC CACAATTCTG
    TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGC
    GGGGTCCTGC AGATCCGCTA CGCTAAGCTG GGTGACACCG GTCGGTACAC CTGCACTGCT
    TCGACCCCCA GTGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGAGTT
    CCAGTTCAAC CTCCAAGACC CACCGACCCC AATTTAATCC CCAGTGCGCC TTCAAAGCCT
    GAAGTCACAG ATGTGAGCAA AAACACAGTC ACTTTATTGT GGCAGCCTAA CCTGAACTCA
    GGAGCAACCC CAACGTCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG
    CAGACCGTGG CAGAAAATGT GAAAACAGAA ACATTCGCCA TCAAAGGGCT CAAACCTAAT
    GCGATCTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GGATTAGCGA TCCAAGCCAA
    ATATCCGATC CAGTGAAAAC ACAAGATGTC CCACCAACCA CCCAAGGGGT GGACCACAAG
    CAGGTCCAGA GAGAACTGGG CAATGTTGTC TTGCATCTCC ACAACCCTAC CATCCTTTCT
    TCCTCTTCTG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGTTAT
    AAAATTCTCT ACCGACCATC TGGAGCCAGC CATGGCGAAT CGGAGTGGTT AGTTTTTGAA
    GTGAGGACCC CAACCAAAAA TAGTGTGGTG ATCCCCGATC TGAGAAAAGG CGTCAACTAC
    GAGATTAAGG CCCGCCCATT TTTTAATGAG TTCCAAGGAG CAGATAGTGA AATCAAATTT
    GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTCACTGT ATCCAAGAAT
    GATGGAAATG GGACAGCAAT TCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAGAAT
    GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGGAATG AGACTAGGTA TCACATAAAC
    AAGACAGTAG ATGGCTCTAC CTTTTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGC
    TACAGTGTAG AAGTGGCTGC AAGCACTGGG GCTGGCCCTG GGGTGAAGAG TGAACCCCAG
    TTCATCCAGT TAGATTCCCA TGGAAACCCC GTGTCTCCCG AGGACCAAGT GAGCCTGGCT
    CAGCAGATCT CCGACGTGGT GAAGCAGCCA GCCTTCATTG CGGGCATCGG GGCCGCCTGT
    TGGATCATTC TCATGGTCTT CAGTATCTGG CTTTACCGAC ACCGCAAGAA GAGAAACGGG
    CTCAGCAGCA CGTACGCGGG GATCCGGAAA GTCCCGTCCT TTACCTTCAC ACCAACAGTA
    ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATC
    AGTGAACCTG CCACACAGCC ATGGCTGGCG GACACGTGGC CCAACACAGG CAACAGTCAC
    AATGACTGCT CCATCAACTG CTGCACAGCC AGCAACGGCA ACAGCGACAG CAACCTGACC
    ACCTACAGTC GCCCAGCTGA TTGTATAGCA AATTATAACA ACCAACTGGA TAACAAACAA
    ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC
    AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGACG GGCGTTTTGT CAATCCATCA
    GGGCAGCCCA CTCCCTACGC CACCACGCAG CTCATCCAGG CAAATCTCAG CAACAATATG
    AATAACGGCG GCGGGGACTC CAGCGAGAAA CACTGGAAGC CTCCGGGGCA GCAGAAACAG
    GAAGTGGCAC CAATTCAGTA TAACATCATG GAGCAAAACA AGCTGAACAA AGATTATCGG
    GCAAATGACA CAATCCCTCC AACCATCCCG TACAACCAAT CCTATGACCA GAATACAGGA
    GGATCTTACA ACAGCTCAGA TCGGGGCAGT AGTACATCTG GGAGTCAAGG CCACAAGAAA
    GGGGCACGGA CACCAAAGGC ACCCAAGCAG GGTGGCATGA ACTGGGCAGA CTTGCTGCCC
    CCTCCCCCAG CGCATCCTCC CCCACACAGC AACAGCGAGG AATACAGCAT GTCTGTAGAT
    GAAAGCTATG ATCAAGAAAT GCCGTGCCCT GTTCCGCCTG CACGGATGTA TTTGCAACAG
    GATGAATTAG AAGAAGAGGA AGCTGAAAGA GGCCCCACGC CCCCTGTCCG GGGGGCAGCT
    TCGTCCCCAG CTGCTGTGTC CTACAGCCAT CAGTCCACGG CCACTCTCAC CCCGTCTCCC
    CAGGAAGAGC TCCAGCCTAT GTTACAGGAT TGTCCGGAGG ACCTCGGCCA CATGCCCCAC
    CCACCAGACA GGAGGCGGCA GCCGGTGAGT CCTCCTCCAC CACCACGGCC AATTTCCCCA
    CCACATACTT ACGGCTACAT TTCAGGACCC CTGGTCTCAG ACATGGATAC GGATGCCCCA
    GAAGAGGAGG AAGACGAAGC TGACATGGAA GTTGCTAAGA TGCAGACCCG AAGGCTGTTG
    TTACGTGGGC TTGAACAGAC CCCAGCATCT AGTGTTGGGG ACCTCGAGAG CTCGGTCACT
    GGCTCCATGA TCAATGGCTG GGGCTCAGCC TCAGAAGAGG ACAACATTTC CAGTGGGCGC
    TCCAGTGTCA GTTCATCGGA TGGTTCCTTT TTCACTGACG CTGATTTTGC CCAGGCAGTT
    GCCGCAGCCG CCGAGTATGC GGGCCTGAAG GTGGCTCGCC GCCAGATGCA GGATGCTGCT
    GGCCGCCGAC ACTTCCATGC CTCCCAGTGC CCTAGGCCCA CGAGTCCTGT GTCTACAGAC
    AGCAACATGA GTGCTGCTGT AATCCAGAAA GCCAGACCCA CCAAGAAACA GAAGCACCAG
    CCAGGACATC TGCGCAGAGA GGCCTACACA GATGATCTTC CACCCCCTCC AGTGCCACCA
    CCTGCTATAA AATCGCCCAG TGTCCAGTCC AAGGCACAGC TGGAGGCACG GCCTATAATG
    GGGCCAAAGC TCGCTTCTAT AGAAGCGAGA GCAGACAGAT CATCAGACAG AAAAGGCGGC
    AGTTACAAGG GGAGAGAAGC TCTGGACGGA AGACAAGTCA CTGACCTGCG AACAAGCCCA
    GGTGACCCCA GAGAAGCACA GGAGCAGCCA AACGAAGGGA AAGCACGAGG GACGAAGACA
    GCAAAGCGAG ACCTTCCACC AGCGAAGACA CACCTCATCC CAGAGGATAT CCTTCCGTAC
    TGTAGGCCTA CTTTCCCAAC GTCAAACAAC CCTAGGGATC CCAGTTCTTC AAGCTCAATG
    TCATCAAGAG GCTCAGGAAG CCGACAGAGA GAGCAAGCAA ATGTGGGCCG AAGAAATATG
    GCAGAAATGC AAGTACTTGG GGGATTTGAA AGAGGAGATG AGAATAATGA AGAATTAGAG
    GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453546.1
    CDS241..5295
    Translation

    Target ORF information:

    RefSeq Version XM_017598057.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598057.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGAGCCAC CACATTTTGT CGTGAAGCCT CGAGACCAGG TGGTTGCCTT GGGGCGAACT
    GTGACTTTTC AGTGTGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GCGGAGAGAG
    GGGAGTCAGA ATCTGCTTTT CTCATACCAG CCTCCACAGT CCTCTAGCCG GTTTTCAGTT
    TCCCAGACGG GGGACCTCAC TGTTACGAAC GTCCAGAGGT CTGATGTCGG TTACTATATC
    TGTCAGACTT TGAACGTGGC TGGAAGCATC ATCACAAAGG CATATTTGGA AGTTACTGAT
    GTGATTGCGG ACCGGCCTCC CCCAGTCATC CGACAAGGTC CTGTGAATCA GACTGTAGCA
    GTGGATGGTA CTTTAACTCT CAGCTGTGTG GCCACAGGCA GTCCCGTGCC CACAATTCTG
    TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGC
    GGGGTCCTGC AGATCCGCTA CGCTAAGCTG GGTGACACCG GTCGGTACAC CTGCACTGCT
    TCGACCCCCA GTGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGAGTT
    CCAGTTCAAC CTCCAAGACC CACCGACCCC AATTTAATCC CCAGTGCGCC TTCAAAGCCT
    GAAGTCACAG ATGTGAGCAA AAACACAGTC ACTTTATTGT GGCAGCCTAA CCTGAACTCA
    GGAGCAACCC CAACGTCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG
    CAGACCGTGG CAGAAAATGT GAAAACAGAA ACATTCGCCA TCAAAGGGCT CAAACCTAAT
    GCGATCTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GGATTAGCGA TCCAAGCCAA
    ATATCCGATC CAGTGAAAAC ACAAGATGTC CCACCAACCA CCCAAGGGGT GGACCACAAG
    CAGGTCCAGA GAGAACTGGG CAATGTTGTC TTGCATCTCC ACAACCCTAC CATCCTTTCT
    TCCTCTTCTG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGTTAT
    AAAATTCTCT ACCGACCATC TGGAGCCAGC CATGGCGAAT CGGAGTGGTT AGTTTTTGAA
    GTGAGGACCC CAACCAAAAA TAGTGTGGTG ATCCCCGATC TGAGAAAAGG CGTCAACTAC
    GAGATTAAGG CCCGCCCATT TTTTAATGAG TTCCAAGGAG CAGATAGTGA AATCAAATTT
    GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTCACTGT ATCCAAGAAT
    GATGGAAATG GGACAGCAAT TCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAGAAT
    GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGGAATG AGACTAGGTA TCACATAAAC
    AAGACAGTAG ATGGCTCTAC CTTTTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGC
    TACAGTGTAG AAGTGGCTGC AAGCACTGGG GCTGGCCCTG GGGTGAAGAG TGAACCCCAG
    TTCATCCAGT TAGATTCCCA TGGAAACCCC GTGTCTCCCG AGGACCAAGT GAGCCTGGCT
    CAGCAGATCT CCGACGTGGT GAAGCAGCCA GCCTTCATTG CGGGCATCGG GGCCGCCTGT
    TGGATCATTC TCATGGTCTT CAGTATCTGG CTTTACCGAC ACCGCAAGAA GAGAAACGGG
    CTCAGCAGCA CGTACGCGGG GATCCGGAAA GTCCCGTCCT TTACCTTCAC ACCAACAGTA
    ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATC
    AGTGAACCTG CCACACAGCC ATGGCTGGCG GACACGTGGC CCAACACAGG CAACAGTCAC
    AATGACTGCT CCATCAACTG CTGCACAGCC AGCAACGGCA ACAGCGACAG CAACCTGACC
    ACCTACAGTC GCCCAGCTGA TTGTATAGCA AATTATAACA ACCAACTGGA TAACAAACAA
    ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC
    AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGACG GGCGTTTTGT CAATCCATCA
    GGGCAGCCCA CTCCCTACGC CACCACGCAG CTCATCCAGG CAAATCTCAG CAACAATATG
    AATAACGGCG GCGGGGACTC CAGCGAGAAA CACTGGAAGC CTCCGGGGCA GCAGAAACAG
    GAAGTGGCAC CAATTCAGTA TAACATCATG GAGCAAAACA AGCTGAACAA AGATTATCGG
    GCAAATGACA CAATCCCTCC AACCATCCCG TACAACCAAT CCTATGACCA GAATACAGGA
    GGATCTTACA ACAGCTCAGA TCGGGGCAGT AGTACATCTG GGAGTCAAGG CCACAAGAAA
    GGGGCACGGA CACCAAAGGC ACCCAAGCAG GGTGGCATGA ACTGGGCAGA CTTGCTGCCC
    CCTCCCCCAG CGCATCCTCC CCCACACAGC AACAGCGAGG AATACAGCAT GTCTGTAGAT
    GAAAGCTATG ATCAAGAAAT GCCGTGCCCT GTTCCGCCTG CACGGATGTA TTTGCAACAG
    GATGAATTAG AAGAAGAGGA AGCTGAAAGA GGCCCCACGC CCCCTGTCCG GGGGGCAGCT
    TCGTCCCCAG CTGCTGTGTC CTACAGCCAT CAGTCCACGG CCACTCTCAC CCCGTCTCCC
    CAGGAAGAGC TCCAGCCTAT GTTACAGGAT TGTCCGGAGG ACCTCGGCCA CATGCCCCAC
    CCACCAGACA GGAGGCGGCA GCCGGTGAGT CCTCCTCCAC CACCACGGCC AATTTCCCCA
    CCACATACTT ACGGCTACAT TTCAGGACCC CTGGTCTCAG ACATGGATAC GGATGCCCCA
    GAAGAGGAGG AAGACGAAGC TGACATGGAA GTTGCTAAGA TGCAGACCCG AAGGCTGTTG
    TTACGTGGGC TTGAACAGAC CCCAGCATCT AGTGTTGGGG ACCTCGAGAG CTCGGTCACT
    GGCTCCATGA TCAATGGCTG GGGCTCAGCC TCAGAAGAGG ACAACATTTC CAGTGGGCGC
    TCCAGTGTCA GTTCATCGGA TGGTTCCTTT TTCACTGACG CTGATTTTGC CCAGGCAGTT
    GCCGCAGCCG CCGAGTATGC GGGCCTGAAG GTGGCTCGCC GCCAGATGCA GGATGCTGCT
    GGCCGCCGAC ACTTCCATGC CTCCCAGTGC CCTAGGCCCA CGAGTCCTGT GTCTACAGAC
    AGCAACATGA GTGCTGCTGT AATCCAGAAA GCCAGACCCA CCAAGAAACA GAAGCACCAG
    CCAGGACATC TGCGCAGAGA GGCCTACACA GATGATCTTC CACCCCCTCC AGTGCCACCA
    CCTGCTATAA AATCGCCCAG TGTCCAGTCC AAGGCACAGC TGGAGGCACG GCCTATAATG
    GGGCCAAAGC TCGCTTCTAT AGAAGCGAGA GCAGACAGAT CATCAGACAG AAAAGGCGGC
    AGTTACAAGG GGAGAGAAGC TCTGGACGGA AGACAAGTCA CTGACCTGCG AACAAGCCCA
    GGTGACCCCA GAGAAGCACA GGAGCAGCCA AACGAAGGGA AAGCACGAGG GACGAAGACA
    GCAAAGCGAG ACCTTCCACC AGCGAAGACA CACCTCATCC CAGAGGATAT CCTTCCGTAC
    TGTAGGCCTA CTTTCCCAAC GTCAAACAAC CCTAGGGATC CCAGTTCTTC AAGCTCAATG
    TCATCAAGAG GCTCAGGAAG CCGACAGAGA GAGCAAGCAA ATGTGGGCCG AAGAAATATG
    GCAGAAATGC AAGTACTTGG GGGATTTGAA AGAGGAGATG AGAATAATGA AGAATTAGAG
    GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57710
    Clone ID Related Accession (Same CDS sequence) XM_017598058.1
    Accession Version XM_017598058.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCACC ACATTTTGTC GTGAAGCCTC GAGACCAGGT GGTTGCCTTG
    GGGCGAACTG TGACTTTTCA GTGTGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    CGGAGAGAGG GGAGTCAGAA TCTGCTTTTC TCATACCAGC CTCCACAGTC CTCTAGCCGG
    TTTTCAGTTT CCCAGACGGG GGACCTCACT GTTACGAACG TCCAGAGGTC TGATGTCGGT
    TACTATATCT GTCAGACTTT GAACGTGGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA
    GTTACTGATG TGATTGCGGA CCGGCCTCCC CCAGTCATCC GACAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGTAC TTTAACTCTC AGCTGTGTGG CCACAGGCAG TCCCGTGCCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGCG GGGTCCTGCA GATCCGCTAC GCTAAGCTGG GTGACACCGG TCGGTACACC
    TGCACTGCTT CGACCCCCAG TGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGAGTTC CAGTTCAACC TCCAAGACCC ACCGACCCCA ATTTAATCCC CAGTGCGCCT
    TCAAAGCCTG AAGTCACAGA TGTGAGCAAA AACACAGTCA CTTTATTGTG GCAGCCTAAC
    CTGAACTCAG GAGCAACCCC AACGTCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACCGTGGC AGAAAATGTG AAAACAGAAA CATTCGCCAT CAAAGGGCTC
    AAACCTAATG CGATCTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG GATTAGCGAT
    CCAAGCCAAA TATCCGATCC AGTGAAAACA CAAGATGTCC CACCAACCAC CCAAGGGGTG
    GACCACAAGC AGGTCCAGAG AGAACTGGGC AATGTTGTCT TGCATCTCCA CAACCCTACC
    ATCCTTTCTT CCTCTTCTGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGTTATA AAATTCTCTA CCGACCATCT GGAGCCAGCC ATGGCGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACCCC AACCAAAAAT AGTGTGGTGA TCCCCGATCT GAGAAAAGGC
    GTCAACTACG AGATTAAGGC CCGCCCATTT TTTAATGAGT TCCAAGGAGC AGATAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTCACTGTA
    TCCAAGAATG ATGGAAATGG GACAGCAATT CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAGAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGGAATGA GACTAGGTAT
    CACATAAACA AGACAGTAGA TGGCTCTACC TTTTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGCT ACAGTGTAGA AGTGGCTGCA AGCACTGGGG CTGGCCCTGG GGTGAAGAGT
    GAACCCCAGT TCATCCAGTT AGATTCCCAT GGAAACCCCG TGTCTCCCGA GGACCAAGTG
    AGCCTGGCTC AGCAGATCTC CGACGTGGTG AAGCAGCCAG CCTTCATTGC GGGCATCGGG
    GCCGCCTGTT GGATCATTCT CATGGTCTTC AGTATCTGGC TTTACCGACA CCGCAAGAAG
    AGAAACGGGC TCAGCAGCAC GTACGCGGGG ATCCGGAAAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TCAGTGAACC TGCCACACAG
    CCATGGCTGG CGGACACGTG GCCCAACACA GGCAACAGTC ACAATGACTG CTCCATCAAC
    TGCTGCACAG CCAGCAACGG CAACAGCGAC AGCAACCTGA CCACCTACAG TCGCCCAGCT
    GATTGTATAG CAAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT
    GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC
    AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAC
    GCCACCACGC AGCTCATCCA GGCAAATCTC AGCAACAATA TGAATAACGG CGGCGGGGAC
    TCCAGCGAGA AACACTGGAA GCCTCCGGGG CAGCAGAAAC AGGAAGTGGC ACCAATTCAG
    TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACAATCCCT
    CCAACCATCC CGTACAACCA ATCCTATGAC CAGAATACAG GAGGATCTTA CAACAGCTCA
    GATCGGGGCA GTAGTACATC TGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG
    GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCCCC AGCGCATCCT
    CCCCCACACA GCAACAGCGA GGAATACAGC ATGTCTGTAG ATGAAAGCTA TGATCAAGAA
    ATGCCGTGCC CTGTTCCGCC TGCACGGATG TATTTGCAAC AGGATGAATT AGAAGAAGAG
    GAAGCTGAAA GAGGCCCCAC GCCCCCTGTC CGGGGGGCAG CTTCGTCCCC AGCTGCTGTG
    TCCTACAGCC ATCAGTCCAC GGCCACTCTC ACCCCGTCTC CCCAGGAAGA GCTCCAGCCT
    ATGTTACAGG ATTGTCCGGA GGACCTCGGC CACATGCCCC ACCCACCAGA CAGGAGGCGG
    CAGCCGGTGA GTCCTCCTCC ACCACCACGG CCAATTTCCC CACCACATAC TTACGGCTAC
    ATTTCAGGAC CCCTGGTCTC AGACATGGAT ACGGATGCCC CAGAAGAGGA GGAAGACGAA
    GCTGACATGG AAGTTGCTAA GATGCAGACC CGAAGGCTGT TGTTACGTGG GCTTGAACAG
    ACCCCAGCAT CTAGTGTTGG GGACCTCGAG AGCTCGGTCA CTGGCTCCAT GATCAATGGC
    TGGGGCTCAG CCTCAGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCG
    GATGGTTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCCGCAGC CGCCGAGTAT
    GCGGGCCTGA AGGTGGCTCG CCGCCAGATG CAGGATGCTG CTGGCCGCCG ACACTTCCAT
    GCCTCCCAGT GCCCTAGGCC CACGAGTCCT GTGTCTACAG ACAGCAACAT GAGTGCTGCT
    GTAATCCAGA AAGCCAGACC CACCAAGAAA CAGAAGCACC AGCCAGGACA TCTGCGCAGA
    GAGGCCTACA CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC
    AGTGTCCAGT CCAAGGCACA GCTGGAGGCA CGGCCTATAA TGGGGCCAAA GCTCGCTTCT
    ATAGAAGCGA GAGCAGACAG ATCATCAGAC AGAAAAGGCG GCAGTTACAA GGGGAGAGAA
    GCTCTGGACG GAAGACAAGT CACTGACCTG CGAACAAGCC CAGGTGACCC CAGAGAAGCA
    CAGGAGCAGC CAAACGAAGG GAAAGCACGA GGGACGAAGA CAGCAAAGCG AGACCTTCCA
    CCAGCGAAGA CACACCTCAT CCCAGAGGAT ATCCTTCCGT ACTGTAGGCC TACTTTCCCA
    ACGTCAAACA ACCCTAGGGA TCCCAGTTCT TCAAGCTCAA TGTCATCAAG AGGCTCAGGA
    AGCCGACAGA GAGAGCAAGC AAATGTGGGC CGAAGAAATA TGGCAGAAAT GCAAGTACTT
    GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453547.1
    CDS241..5277
    Translation

    Target ORF information:

    RefSeq Version XM_017598058.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598058.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCACC ACATTTTGTC GTGAAGCCTC GAGACCAGGT GGTTGCCTTG
    GGGCGAACTG TGACTTTTCA GTGTGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    CGGAGAGAGG GGAGTCAGAA TCTGCTTTTC TCATACCAGC CTCCACAGTC CTCTAGCCGG
    TTTTCAGTTT CCCAGACGGG GGACCTCACT GTTACGAACG TCCAGAGGTC TGATGTCGGT
    TACTATATCT GTCAGACTTT GAACGTGGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA
    GTTACTGATG TGATTGCGGA CCGGCCTCCC CCAGTCATCC GACAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGTAC TTTAACTCTC AGCTGTGTGG CCACAGGCAG TCCCGTGCCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGCG GGGTCCTGCA GATCCGCTAC GCTAAGCTGG GTGACACCGG TCGGTACACC
    TGCACTGCTT CGACCCCCAG TGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGAGTTC CAGTTCAACC TCCAAGACCC ACCGACCCCA ATTTAATCCC CAGTGCGCCT
    TCAAAGCCTG AAGTCACAGA TGTGAGCAAA AACACAGTCA CTTTATTGTG GCAGCCTAAC
    CTGAACTCAG GAGCAACCCC AACGTCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACCGTGGC AGAAAATGTG AAAACAGAAA CATTCGCCAT CAAAGGGCTC
    AAACCTAATG CGATCTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG GATTAGCGAT
    CCAAGCCAAA TATCCGATCC AGTGAAAACA CAAGATGTCC CACCAACCAC CCAAGGGGTG
    GACCACAAGC AGGTCCAGAG AGAACTGGGC AATGTTGTCT TGCATCTCCA CAACCCTACC
    ATCCTTTCTT CCTCTTCTGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGTTATA AAATTCTCTA CCGACCATCT GGAGCCAGCC ATGGCGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACCCC AACCAAAAAT AGTGTGGTGA TCCCCGATCT GAGAAAAGGC
    GTCAACTACG AGATTAAGGC CCGCCCATTT TTTAATGAGT TCCAAGGAGC AGATAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTCACTGTA
    TCCAAGAATG ATGGAAATGG GACAGCAATT CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAGAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGGAATGA GACTAGGTAT
    CACATAAACA AGACAGTAGA TGGCTCTACC TTTTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGCT ACAGTGTAGA AGTGGCTGCA AGCACTGGGG CTGGCCCTGG GGTGAAGAGT
    GAACCCCAGT TCATCCAGTT AGATTCCCAT GGAAACCCCG TGTCTCCCGA GGACCAAGTG
    AGCCTGGCTC AGCAGATCTC CGACGTGGTG AAGCAGCCAG CCTTCATTGC GGGCATCGGG
    GCCGCCTGTT GGATCATTCT CATGGTCTTC AGTATCTGGC TTTACCGACA CCGCAAGAAG
    AGAAACGGGC TCAGCAGCAC GTACGCGGGG ATCCGGAAAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TCAGTGAACC TGCCACACAG
    CCATGGCTGG CGGACACGTG GCCCAACACA GGCAACAGTC ACAATGACTG CTCCATCAAC
    TGCTGCACAG CCAGCAACGG CAACAGCGAC AGCAACCTGA CCACCTACAG TCGCCCAGCT
    GATTGTATAG CAAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT
    GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC
    AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAC
    GCCACCACGC AGCTCATCCA GGCAAATCTC AGCAACAATA TGAATAACGG CGGCGGGGAC
    TCCAGCGAGA AACACTGGAA GCCTCCGGGG CAGCAGAAAC AGGAAGTGGC ACCAATTCAG
    TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACAATCCCT
    CCAACCATCC CGTACAACCA ATCCTATGAC CAGAATACAG GAGGATCTTA CAACAGCTCA
    GATCGGGGCA GTAGTACATC TGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG
    GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCCCC AGCGCATCCT
    CCCCCACACA GCAACAGCGA GGAATACAGC ATGTCTGTAG ATGAAAGCTA TGATCAAGAA
    ATGCCGTGCC CTGTTCCGCC TGCACGGATG TATTTGCAAC AGGATGAATT AGAAGAAGAG
    GAAGCTGAAA GAGGCCCCAC GCCCCCTGTC CGGGGGGCAG CTTCGTCCCC AGCTGCTGTG
    TCCTACAGCC ATCAGTCCAC GGCCACTCTC ACCCCGTCTC CCCAGGAAGA GCTCCAGCCT
    ATGTTACAGG ATTGTCCGGA GGACCTCGGC CACATGCCCC ACCCACCAGA CAGGAGGCGG
    CAGCCGGTGA GTCCTCCTCC ACCACCACGG CCAATTTCCC CACCACATAC TTACGGCTAC
    ATTTCAGGAC CCCTGGTCTC AGACATGGAT ACGGATGCCC CAGAAGAGGA GGAAGACGAA
    GCTGACATGG AAGTTGCTAA GATGCAGACC CGAAGGCTGT TGTTACGTGG GCTTGAACAG
    ACCCCAGCAT CTAGTGTTGG GGACCTCGAG AGCTCGGTCA CTGGCTCCAT GATCAATGGC
    TGGGGCTCAG CCTCAGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCG
    GATGGTTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCCGCAGC CGCCGAGTAT
    GCGGGCCTGA AGGTGGCTCG CCGCCAGATG CAGGATGCTG CTGGCCGCCG ACACTTCCAT
    GCCTCCCAGT GCCCTAGGCC CACGAGTCCT GTGTCTACAG ACAGCAACAT GAGTGCTGCT
    GTAATCCAGA AAGCCAGACC CACCAAGAAA CAGAAGCACC AGCCAGGACA TCTGCGCAGA
    GAGGCCTACA CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC
    AGTGTCCAGT CCAAGGCACA GCTGGAGGCA CGGCCTATAA TGGGGCCAAA GCTCGCTTCT
    ATAGAAGCGA GAGCAGACAG ATCATCAGAC AGAAAAGGCG GCAGTTACAA GGGGAGAGAA
    GCTCTGGACG GAAGACAAGT CACTGACCTG CGAACAAGCC CAGGTGACCC CAGAGAAGCA
    CAGGAGCAGC CAAACGAAGG GAAAGCACGA GGGACGAAGA CAGCAAAGCG AGACCTTCCA
    CCAGCGAAGA CACACCTCAT CCCAGAGGAT ATCCTTCCGT ACTGTAGGCC TACTTTCCCA
    ACGTCAAACA ACCCTAGGGA TCCCAGTTCT TCAAGCTCAA TGTCATCAAG AGGCTCAGGA
    AGCCGACAGA GAGAGCAAGC AAATGTGGGC CGAAGAAATA TGGCAGAAAT GCAAGTACTT
    GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57711
    Clone ID Related Accession (Same CDS sequence) XM_017598059.1
    Accession Version XM_017598059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATCTTCC TCTTCTGGTC ATGATATCTC TCCTCACTCT ATCAAAAAAG 
    CATCTTCTTC TGGCCCAGCT TATTCCAGAC CCTGAGGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAG CCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTCCC ACCGCGCATT GTTGAGCACC CTTCAGACCT GATCGTCTCT
    AAAGGGGAAC CCGCCACCCT CAACTGTAAA GCTGAAGGCC GCCCTACCCC GACTATAGAA
    TGGTACAAGG GCGGCGAAAG AGTGGAAACG GACAAAGACG ACCCTCGCTC ACACCGAATG
    CTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGCATAGTGC ATGGACGGAA GAGTAGGCCT
    GACGAAGGGG TCTACATCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGCCACAAT
    GCATCGCTGG AGGTAGCTAT ACTACGGGAT GACTTCAGAC AAAACCCTTC AGATGTTATG
    GTGGCGGTGG GCGAGCCTGC AGTAATGGAA TGCCAGCCTC CTCGGGGCCA CCCAGAGCCC
    ACCATCTCCT GGAAAAAGGA TGGCTCACCC CTGGATGATA AAGACGAGCG AATCACTATC
    CGAGGCGGGA AGCTCATGAT CACGTACACC CGTAAAAGCG ATGCTGGCAA GTATGTTTGT
    GTTGGTACCA ACATGGTAGG GGAGCGGGAG AGCGAAGTCG CGGAGCTGAC TGTCTTAGAG
    AGACCGTCCT TTGTAAAGAG ACCCAGTAAT TTGGCAGTGA CTGTGGATGA CAGTGCTGAG
    TTTAAGTGTG AGGCCCGAGG AGACCCCGTG CCTACGGTTC GGTGGAGGAA AGATGACGGA
    GAGCTGCCCA AATCCAGATA TGAGATCCGA GATGATCATA CTTTGAAAAT TCGGAAGGTG
    ACAGCTGGCG ACATGGGATC ATACACTTGT GTTGCAGAAA ATATGGTTGG GAAAGCCGAA
    GCATCAGCCA CTTTGACAGT TCAAGTTGGG TCTGAGCCAC CACATTTTGT CGTGAAGCCT
    CGAGACCAGG TGGTTGCCTT GGGGCGAACT GTGACTTTTC AGTGTGAAGC AACTGGAAAT
    CCTCAACCAG CTATCTTTTG GCGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATACCAG
    CCTCCACAGT CCTCTAGCCG GTTTTCAGTT TCCCAGACGG GGGACCTCAC TGTTACGAAC
    GTCCAGAGGT CTGATGTCGG TTACTATATC TGTCAGACTT TGAACGTGGC TGGAAGCATC
    ATCACAAAGG CATATTTGGA AGTTACTGAT GTGATTGCGG ACCGGCCTCC CCCAGTCATC
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAACTCT CAGCTGTGTG
    GCCACAGGCA GTCCCGTGCC CACAATTCTG TGGAGGAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GGATCAAACA GCTGGAGAGC GGGGTCCTGC AGATCCGCTA CGCTAAGCTG
    GGTGACACCG GTCGGTACAC CTGCACTGCT TCGACCCCCA GTGGTGAGGC CACGTGGAGC
    GCTTACATCG AAGTCCAGGA ATTTGGAGTT CCAGTTCAAC CTCCAAGACC CACCGACCCC
    AATTTAATCC CCAGTGCGCC TTCAAAGCCT GAAGTCACAG ATGTGAGCAA AAACACAGTC
    ACTTTATTGT GGCAGCCTAA CCTGAACTCA GGAGCAACCC CAACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTGG CAGAAAATGT GAAAACAGAA
    ACATTCGCCA TCAAAGGGCT CAAACCTAAT GCGATCTACC TTTTCCTGGT GAGGGCAGCA
    AATGCCTATG GGATTAGCGA TCCAAGCCAA ATATCCGATC CAGTGAAAAC ACAAGATGTC
    CCACCAACCA CCCAAGGGGT GGACCACAAG CAGGTCCAGA GAGAACTGGG CAATGTTGTC
    TTGCATCTCC ACAACCCTAC CATCCTTTCT TCCTCTTCTG TCGAAGTGCA CTGGACGGTG
    GACCAACAAT CTCAATATAT TCAAGGTTAT AAAATTCTCT ACCGACCATC TGGAGCCAGC
    CATGGCGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA TAGTGTGGTG
    ATCCCCGATC TGAGAAAAGG CGTCAACTAC GAGATTAAGG CCCGCCCATT TTTTAATGAG
    TTCCAAGGAG CAGATAGTGA AATCAAATTT GCCAAAACCT TAGAAGAAGC ACCAAGTGCC
    CCTCCCCGAA GTGTCACTGT ATCCAAGAAT GATGGAAATG GGACAGCAAT TCTGGTCACT
    TGGCAACCGC CTCCTGAAGA CACACAGAAT GGAATGGTCC AAGAATATAA GGTTTGGTGT
    CTCGGGAATG AGACTAGGTA TCACATAAAC AAGACAGTAG ATGGCTCTAC CTTTTCGGTG
    GTCATCCCCT CTCTGGTTCC TGGGATCCGC TACAGTGTAG AAGTGGCTGC AAGCACTGGG
    GCTGGCCCTG GGGTGAAGAG TGAACCCCAG TTCATCCAGT TAGATTCCCA TGGAAACCCC
    GTGTCTCCCG AGGACCAAGT GAGCCTGGCT CAGCAGATCT CCGACGTGGT GAAGCAGCCA
    GCCTTCATTG CGGGCATCGG GGCCGCCTGT TGGATCATTC TCATGGTCTT CAGTATCTGG
    CTTTACCGAC ACCGCAAGAA GAGAAACGGG CTCAGCAGCA CGTACGCGGG GATCCGGAAA
    GTCCCGTCCT TTACCTTCAC ACCAACAGTA ACTTATCAAA GAGGAGGCGA AGCCGTCAGC
    AGTGGAGGGA GGCCGGGACT CCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTGGCG
    GACACGTGGC CCAACACAGG CAACAGTCAC AATGACTGCT CCATCAACTG CTGCACAGCC
    AGCAACGGCA ACAGCGACAG CAACCTGACC ACCTACAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGACG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CTCCCTACGC CACCACGCAG
    CTCATCCAGG CAAATCTCAG CAACAATATG AATAACGGCG GCGGGGACTC CAGCGAGAAA
    CACTGGAAGC CTCCGGGGCA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AGCTGAACAA AGATTATCGG GCAAATGACA CAATCCCTCC AACCATCCCG
    TACAACCAAT CCTATGACCA GAATACAGGA GGATCTTACA ACAGCTCAGA TCGGGGCAGT
    AGTACATCTG GGAGTCAAGG CCACAAGAAA GGGGCACGGA CACCAAAGGC ACCCAAGCAG
    GGTGGCATGA ACTGGGCAGA CTTGCTGCCC CCTCCCCCAG CGCATCCTCC CCCACACAGC
    AACAGCGAGG AATACAGCAT GTCTGTAGAT GAAAGCTATG ATCAAGAAAT GCCGTGCCCT
    GTTCCGCCTG CACGGATGTA TTTGCAACAG GATGAATTAG AAGAAGAGGA AGCTGAAAGA
    GGCCCCACGC CCCCTGTCCG GGGGGCAGCT TCGTCCCCAG CTGCTGTGTC CTACAGCCAT
    CAGTCCACGG CCACTCTCAC CCCGTCTCCC CAGGAAGAGC TCCAGCCTAT GTTACAGGAT
    TGTCCGGAGG ACCTCGGCCA CATGCCCCAC CCACCAGACA GGAGGCGGCA GCCGGTGAGT
    CCTCCTCCAC CACCACGGCC AATTTCCCCA CCACATACTT ACGGCTACAT TTCAGGACCC
    CTGGTCTCAG ACATGGATAC GGATGCCCCA GAAGAGGAGG AAGACGAAGC TGACATGGAA
    GTTGCTAAGA TGCAGACCCG AAGGCTGTTG TTACGTGGGC TTGAACAGAC CCCAGCATCT
    AGTGTTGGGG ACCTCGAGAG CTCGGTCACT GGCTCCATGA TCAATGGCTG GGGCTCAGCC
    TCAGAAGAGG ACAACATTTC CAGTGGGCGC TCCAGTGTCA GTTCATCGGA TGGTTCCTTT
    TTCACTGACG CTGATTTTGC CCAGGCAGTT GCCGCAGCCG CCGAGTATGC GGGCCTGAAG
    GTGGCTCGCC GCCAGATGCA GGATGCTGCT GGCCGCCGAC ACTTCCATGC CTCCCAGTGC
    CCTAGGCCCA CGAGTCCTGT GTCTACAGAC AGCAACATGA GTGCTGCTGT AATCCAGAAA
    GCCAGACCCA CCAAGAAACA GAAGCACCAG CCAGGACATC TGCGCAGAGA GGCCTACACA
    GATGATCTTC CACCCCCTCC AGTGCCACCA CCTGCTATAA AATCGCCCAG TGTCCAGTCC
    AAGGCACAGC TGGAGGCACG GCCTATAATG GGGCCAAAGC TCGCTTCTAT AGAAGCGAGA
    GCAGACAGAT CATCAGACAG AAAAGGCGGC AGTTACAAGG GGAGAGAAGC TCTGGACGGA
    AGACAAGTCA CTGACCTGCG AACAAGCCCA GGTGACCCCA GAGAAGCACA GGAGCAGCCA
    AACGAAGGGA AAGCACGAGG GACGAAGACA GCAAAGCGAG ACCTTCCACC AGCGAAGACA
    CACCTCATCC CAGAGGATAT CCTTCCGTAC TGTAGGCCTA CTTTCCCAAC GTCAAACAAC
    CCTAGGGATC CCAGTTCTTC AAGCTCAATG TCATCAAGAG GCTCAGGAAG CCGACAGAGA
    GAGCAAGCAA ATGTGGGCCG AAGAAATATG GCAGAAATGC AAGTACTTGG GGGATTTGAA
    AGAGGAGATG AGAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453548.1
    CDS371..5335
    Translation

    Target ORF information:

    RefSeq Version XM_017598059.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATCTTCC TCTTCTGGTC ATGATATCTC TCCTCACTCT ATCAAAAAAG 
    CATCTTCTTC TGGCCCAGCT TATTCCAGAC CCTGAGGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAG CCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTCCC ACCGCGCATT GTTGAGCACC CTTCAGACCT GATCGTCTCT
    AAAGGGGAAC CCGCCACCCT CAACTGTAAA GCTGAAGGCC GCCCTACCCC GACTATAGAA
    TGGTACAAGG GCGGCGAAAG AGTGGAAACG GACAAAGACG ACCCTCGCTC ACACCGAATG
    CTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGCATAGTGC ATGGACGGAA GAGTAGGCCT
    GACGAAGGGG TCTACATCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGCCACAAT
    GCATCGCTGG AGGTAGCTAT ACTACGGGAT GACTTCAGAC AAAACCCTTC AGATGTTATG
    GTGGCGGTGG GCGAGCCTGC AGTAATGGAA TGCCAGCCTC CTCGGGGCCA CCCAGAGCCC
    ACCATCTCCT GGAAAAAGGA TGGCTCACCC CTGGATGATA AAGACGAGCG AATCACTATC
    CGAGGCGGGA AGCTCATGAT CACGTACACC CGTAAAAGCG ATGCTGGCAA GTATGTTTGT
    GTTGGTACCA ACATGGTAGG GGAGCGGGAG AGCGAAGTCG CGGAGCTGAC TGTCTTAGAG
    AGACCGTCCT TTGTAAAGAG ACCCAGTAAT TTGGCAGTGA CTGTGGATGA CAGTGCTGAG
    TTTAAGTGTG AGGCCCGAGG AGACCCCGTG CCTACGGTTC GGTGGAGGAA AGATGACGGA
    GAGCTGCCCA AATCCAGATA TGAGATCCGA GATGATCATA CTTTGAAAAT TCGGAAGGTG
    ACAGCTGGCG ACATGGGATC ATACACTTGT GTTGCAGAAA ATATGGTTGG GAAAGCCGAA
    GCATCAGCCA CTTTGACAGT TCAAGTTGGG TCTGAGCCAC CACATTTTGT CGTGAAGCCT
    CGAGACCAGG TGGTTGCCTT GGGGCGAACT GTGACTTTTC AGTGTGAAGC AACTGGAAAT
    CCTCAACCAG CTATCTTTTG GCGGAGAGAG GGGAGTCAGA ATCTGCTTTT CTCATACCAG
    CCTCCACAGT CCTCTAGCCG GTTTTCAGTT TCCCAGACGG GGGACCTCAC TGTTACGAAC
    GTCCAGAGGT CTGATGTCGG TTACTATATC TGTCAGACTT TGAACGTGGC TGGAAGCATC
    ATCACAAAGG CATATTTGGA AGTTACTGAT GTGATTGCGG ACCGGCCTCC CCCAGTCATC
    CGACAAGGTC CTGTGAATCA GACTGTAGCA GTGGATGGTA CTTTAACTCT CAGCTGTGTG
    GCCACAGGCA GTCCCGTGCC CACAATTCTG TGGAGGAAGG ATGGAGTCCT TGTTTCAACC
    CAAGATTCTC GGATCAAACA GCTGGAGAGC GGGGTCCTGC AGATCCGCTA CGCTAAGCTG
    GGTGACACCG GTCGGTACAC CTGCACTGCT TCGACCCCCA GTGGTGAGGC CACGTGGAGC
    GCTTACATCG AAGTCCAGGA ATTTGGAGTT CCAGTTCAAC CTCCAAGACC CACCGACCCC
    AATTTAATCC CCAGTGCGCC TTCAAAGCCT GAAGTCACAG ATGTGAGCAA AAACACAGTC
    ACTTTATTGT GGCAGCCTAA CCTGAACTCA GGAGCAACCC CAACGTCTTA TATTATAGAA
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAGACCGTGG CAGAAAATGT GAAAACAGAA
    ACATTCGCCA TCAAAGGGCT CAAACCTAAT GCGATCTACC TTTTCCTGGT GAGGGCAGCA
    AATGCCTATG GGATTAGCGA TCCAAGCCAA ATATCCGATC CAGTGAAAAC ACAAGATGTC
    CCACCAACCA CCCAAGGGGT GGACCACAAG CAGGTCCAGA GAGAACTGGG CAATGTTGTC
    TTGCATCTCC ACAACCCTAC CATCCTTTCT TCCTCTTCTG TCGAAGTGCA CTGGACGGTG
    GACCAACAAT CTCAATATAT TCAAGGTTAT AAAATTCTCT ACCGACCATC TGGAGCCAGC
    CATGGCGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA TAGTGTGGTG
    ATCCCCGATC TGAGAAAAGG CGTCAACTAC GAGATTAAGG CCCGCCCATT TTTTAATGAG
    TTCCAAGGAG CAGATAGTGA AATCAAATTT GCCAAAACCT TAGAAGAAGC ACCAAGTGCC
    CCTCCCCGAA GTGTCACTGT ATCCAAGAAT GATGGAAATG GGACAGCAAT TCTGGTCACT
    TGGCAACCGC CTCCTGAAGA CACACAGAAT GGAATGGTCC AAGAATATAA GGTTTGGTGT
    CTCGGGAATG AGACTAGGTA TCACATAAAC AAGACAGTAG ATGGCTCTAC CTTTTCGGTG
    GTCATCCCCT CTCTGGTTCC TGGGATCCGC TACAGTGTAG AAGTGGCTGC AAGCACTGGG
    GCTGGCCCTG GGGTGAAGAG TGAACCCCAG TTCATCCAGT TAGATTCCCA TGGAAACCCC
    GTGTCTCCCG AGGACCAAGT GAGCCTGGCT CAGCAGATCT CCGACGTGGT GAAGCAGCCA
    GCCTTCATTG CGGGCATCGG GGCCGCCTGT TGGATCATTC TCATGGTCTT CAGTATCTGG
    CTTTACCGAC ACCGCAAGAA GAGAAACGGG CTCAGCAGCA CGTACGCGGG GATCCGGAAA
    GTCCCGTCCT TTACCTTCAC ACCAACAGTA ACTTATCAAA GAGGAGGCGA AGCCGTCAGC
    AGTGGAGGGA GGCCGGGACT CCTCAACATC AGTGAACCTG CCACACAGCC ATGGCTGGCG
    GACACGTGGC CCAACACAGG CAACAGTCAC AATGACTGCT CCATCAACTG CTGCACAGCC
    AGCAACGGCA ACAGCGACAG CAACCTGACC ACCTACAGTC GCCCAGCTGA TTGTATAGCA
    AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT
    TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT
    CTGAAGGACG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CTCCCTACGC CACCACGCAG
    CTCATCCAGG CAAATCTCAG CAACAATATG AATAACGGCG GCGGGGACTC CAGCGAGAAA
    CACTGGAAGC CTCCGGGGCA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG
    GAGCAAAACA AGCTGAACAA AGATTATCGG GCAAATGACA CAATCCCTCC AACCATCCCG
    TACAACCAAT CCTATGACCA GAATACAGGA GGATCTTACA ACAGCTCAGA TCGGGGCAGT
    AGTACATCTG GGAGTCAAGG CCACAAGAAA GGGGCACGGA CACCAAAGGC ACCCAAGCAG
    GGTGGCATGA ACTGGGCAGA CTTGCTGCCC CCTCCCCCAG CGCATCCTCC CCCACACAGC
    AACAGCGAGG AATACAGCAT GTCTGTAGAT GAAAGCTATG ATCAAGAAAT GCCGTGCCCT
    GTTCCGCCTG CACGGATGTA TTTGCAACAG GATGAATTAG AAGAAGAGGA AGCTGAAAGA
    GGCCCCACGC CCCCTGTCCG GGGGGCAGCT TCGTCCCCAG CTGCTGTGTC CTACAGCCAT
    CAGTCCACGG CCACTCTCAC CCCGTCTCCC CAGGAAGAGC TCCAGCCTAT GTTACAGGAT
    TGTCCGGAGG ACCTCGGCCA CATGCCCCAC CCACCAGACA GGAGGCGGCA GCCGGTGAGT
    CCTCCTCCAC CACCACGGCC AATTTCCCCA CCACATACTT ACGGCTACAT TTCAGGACCC
    CTGGTCTCAG ACATGGATAC GGATGCCCCA GAAGAGGAGG AAGACGAAGC TGACATGGAA
    GTTGCTAAGA TGCAGACCCG AAGGCTGTTG TTACGTGGGC TTGAACAGAC CCCAGCATCT
    AGTGTTGGGG ACCTCGAGAG CTCGGTCACT GGCTCCATGA TCAATGGCTG GGGCTCAGCC
    TCAGAAGAGG ACAACATTTC CAGTGGGCGC TCCAGTGTCA GTTCATCGGA TGGTTCCTTT
    TTCACTGACG CTGATTTTGC CCAGGCAGTT GCCGCAGCCG CCGAGTATGC GGGCCTGAAG
    GTGGCTCGCC GCCAGATGCA GGATGCTGCT GGCCGCCGAC ACTTCCATGC CTCCCAGTGC
    CCTAGGCCCA CGAGTCCTGT GTCTACAGAC AGCAACATGA GTGCTGCTGT AATCCAGAAA
    GCCAGACCCA CCAAGAAACA GAAGCACCAG CCAGGACATC TGCGCAGAGA GGCCTACACA
    GATGATCTTC CACCCCCTCC AGTGCCACCA CCTGCTATAA AATCGCCCAG TGTCCAGTCC
    AAGGCACAGC TGGAGGCACG GCCTATAATG GGGCCAAAGC TCGCTTCTAT AGAAGCGAGA
    GCAGACAGAT CATCAGACAG AAAAGGCGGC AGTTACAAGG GGAGAGAAGC TCTGGACGGA
    AGACAAGTCA CTGACCTGCG AACAAGCCCA GGTGACCCCA GAGAAGCACA GGAGCAGCCA
    AACGAAGGGA AAGCACGAGG GACGAAGACA GCAAAGCGAG ACCTTCCACC AGCGAAGACA
    CACCTCATCC CAGAGGATAT CCTTCCGTAC TGTAGGCCTA CTTTCCCAAC GTCAAACAAC
    CCTAGGGATC CCAGTTCTTC AAGCTCAATG TCATCAAGAG GCTCAGGAAG CCGACAGAGA
    GAGCAAGCAA ATGTGGGCCG AAGAAATATG GCAGAAATGC AAGTACTTGG GGGATTTGAA
    AGAGGAGATG AGAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57712
    Clone ID Related Accession (Same CDS sequence) XM_017598060.1
    Accession Version XM_017598060.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATCTTCC TCTTCTGGTC ATGATATCTC TCCTCACTCT ATCAAAAAAG 
    CATCTTCTTC TGGCCCAGCT TATTCCAGAC CCTGAGGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAG CCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTCCC ACCGCGCATT GTTGAGCACC CTTCAGACCT GATCGTCTCT
    AAAGGGGAAC CCGCCACCCT CAACTGTAAA GCTGAAGGCC GCCCTACCCC GACTATAGAA
    TGGTACAAGG GCGGCGAAAG AGTGGAAACG GACAAAGACG ACCCTCGCTC ACACCGAATG
    CTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGCATAGTGC ATGGACGGAA GAGTAGGCCT
    GACGAAGGGG TCTACATCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGCCACAAT
    GCATCGCTGG AGGTAGCTAT ACTACGGGAT GACTTCAGAC AAAACCCTTC AGATGTTATG
    GTGGCGGTGG GCGAGCCTGC AGTAATGGAA TGCCAGCCTC CTCGGGGCCA CCCAGAGCCC
    ACCATCTCCT GGAAAAAGGA TGGCTCACCC CTGGATGATA AAGACGAGCG AATCACTATC
    CGAGGCGGGA AGCTCATGAT CACGTACACC CGTAAAAGCG ATGCTGGCAA GTATGTTTGT
    GTTGGTACCA ACATGGTAGG GGAGCGGGAG AGCGAAGTCG CGGAGCTGAC TGTCTTAGAG
    AGACCGTCCT TTGTAAAGAG ACCCAGTAAT TTGGCAGTGA CTGTGGATGA CAGTGCTGAG
    TTTAAGTGTG AGGCCCGAGG AGACCCCGTG CCTACGGTTC GGTGGAGGAA AGATGACGGA
    GAGCTGCCCA AATCCAGATA TGAGATCCGA GATGATCATA CTTTGAAAAT TCGGAAGGTG
    ACAGCTGGCG ACATGGGATC ATACACTTGT GTTGCAGAAA ATATGGTTGG GAAAGCCGAA
    GCATCAGCCA CTTTGACAGT TCAAGAGCCA CCACATTTTG TCGTGAAGCC TCGAGACCAG
    GTGGTTGCCT TGGGGCGAAC TGTGACTTTT CAGTGTGAAG CAACTGGAAA TCCTCAACCA
    GCTATCTTTT GGCGGAGAGA GGGGAGTCAG AATCTGCTTT TCTCATACCA GCCTCCACAG
    TCCTCTAGCC GGTTTTCAGT TTCCCAGACG GGGGACCTCA CTGTTACGAA CGTCCAGAGG
    TCTGATGTCG GTTACTATAT CTGTCAGACT TTGAACGTGG CTGGAAGCAT CATCACAAAG
    GCATATTTGG AAGTTACTGA TGTGATTGCG GACCGGCCTC CCCCAGTCAT CCGACAAGGT
    CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAACTC TCAGCTGTGT GGCCACAGGC
    AGTCCCGTGC CCACAATTCT GTGGAGGAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT
    CGGATCAAAC AGCTGGAGAG CGGGGTCCTG CAGATCCGCT ACGCTAAGCT GGGTGACACC
    GGTCGGTACA CCTGCACTGC TTCGACCCCC AGTGGTGAGG CCACGTGGAG CGCTTACATC
    GAAGTCCAGG AATTTGGAGT TCCAGTTCAA CCTCCAAGAC CCACCGACCC CAATTTAATC
    CCCAGTGCGC CTTCAAAGCC TGAAGTCACA GATGTGAGCA AAAACACAGT CACTTTATTG
    TGGCAGCCTA ACCTGAACTC AGGAGCAACC CCAACGTCTT ATATTATAGA AGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAGACCGTG GCAGAAAATG TGAAAACAGA AACATTCGCC
    ATCAAAGGGC TCAAACCTAA TGCGATCTAC CTTTTCCTGG TGAGGGCAGC AAATGCCTAT
    GGGATTAGCG ATCCAAGCCA AATATCCGAT CCAGTGAAAA CACAAGATGT CCCACCAACC
    ACCCAAGGGG TGGACCACAA GCAGGTCCAG AGAGAACTGG GCAATGTTGT CTTGCATCTC
    CACAACCCTA CCATCCTTTC TTCCTCTTCT GTCGAAGTGC ACTGGACGGT GGACCAACAA
    TCTCAATATA TTCAAGGTTA TAAAATTCTC TACCGACCAT CTGGAGCCAG CCATGGCGAA
    TCGGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ATAGTGTGGT GATCCCCGAT
    CTGAGAAAAG GCGTCAACTA CGAGATTAAG GCCCGCCCAT TTTTTAATGA GTTCCAAGGA
    GCAGATAGTG AAATCAAATT TGCCAAAACC TTAGAAGAAG CACCAAGTGC CCCTCCCCGA
    AGTGTCACTG TATCCAAGAA TGATGGAAAT GGGACAGCAA TTCTGGTCAC TTGGCAACCG
    CCTCCTGAAG ACACACAGAA TGGAATGGTC CAAGAATATA AGGTTTGGTG TCTCGGGAAT
    GAGACTAGGT ATCACATAAA CAAGACAGTA GATGGCTCTA CCTTTTCGGT GGTCATCCCC
    TCTCTGGTTC CTGGGATCCG CTACAGTGTA GAAGTGGCTG CAAGCACTGG GGCTGGCCCT
    GGGGTGAAGA GTGAACCCCA GTTCATCCAG TTAGATTCCC ATGGAAACCC CGTGTCTCCC
    GAGGACCAAG TGAGCCTGGC TCAGCAGATC TCCGACGTGG TGAAGCAGCC AGCCTTCATT
    GCGGGCATCG GGGCCGCCTG TTGGATCATT CTCATGGTCT TCAGTATCTG GCTTTACCGA
    CACCGCAAGA AGAGAAACGG GCTCAGCAGC ACGTACGCGG GGATCCGGAA AGTCCCGTCC
    TTTACCTTCA CACCAACAGT AACTTATCAA AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG
    AGGCCGGGAC TCCTCAACAT CAGTGAACCT GCCACACAGC CATGGCTGGC GGACACGTGG
    CCCAACACAG GCAACAGTCA CAATGACTGC TCCATCAACT GCTGCACAGC CAGCAACGGC
    AACAGCGACA GCAACCTGAC CACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC
    AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT
    GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAC
    GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCCTACG CCACCACGCA GCTCATCCAG
    GCAAATCTCA GCAACAATAT GAATAACGGC GGCGGGGACT CCAGCGAGAA ACACTGGAAG
    CCTCCGGGGC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAGCTGAACA AAGATTATCG GGCAAATGAC ACAATCCCTC CAACCATCCC GTACAACCAA
    TCCTATGACC AGAATACAGG AGGATCTTAC AACAGCTCAG ATCGGGGCAG TAGTACATCT
    GGGAGTCAAG GCCACAAGAA AGGGGCACGG ACACCAAAGG CACCCAAGCA GGGTGGCATG
    AACTGGGCAG ACTTGCTGCC CCCTCCCCCA GCGCATCCTC CCCCACACAG CAACAGCGAG
    GAATACAGCA TGTCTGTAGA TGAAAGCTAT GATCAAGAAA TGCCGTGCCC TGTTCCGCCT
    GCACGGATGT ATTTGCAACA GGATGAATTA GAAGAAGAGG AAGCTGAAAG AGGCCCCACG
    CCCCCTGTCC GGGGGGCAGC TTCGTCCCCA GCTGCTGTGT CCTACAGCCA TCAGTCCACG
    GCCACTCTCA CCCCGTCTCC CCAGGAAGAG CTCCAGCCTA TGTTACAGGA TTGTCCGGAG
    GACCTCGGCC ACATGCCCCA CCCACCAGAC AGGAGGCGGC AGCCGGTGAG TCCTCCTCCA
    CCACCACGGC CAATTTCCCC ACCACATACT TACGGCTACA TTTCAGGACC CCTGGTCTCA
    GACATGGATA CGGATGCCCC AGAAGAGGAG GAAGACGAAG CTGACATGGA AGTTGCTAAG
    ATGCAGACCC GAAGGCTGTT GTTACGTGGG CTTGAACAGA CCCCAGCATC TAGTGTTGGG
    GACCTCGAGA GCTCGGTCAC TGGCTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAAGAG
    GACAACATTT CCAGTGGGCG CTCCAGTGTC AGTTCATCGG ATGGTTCCTT TTTCACTGAC
    GCTGATTTTG CCCAGGCAGT TGCCGCAGCC GCCGAGTATG CGGGCCTGAA GGTGGCTCGC
    CGCCAGATGC AGGATGCTGC TGGCCGCCGA CACTTCCATG CCTCCCAGTG CCCTAGGCCC
    ACGAGTCCTG TGTCTACAGA CAGCAACATG AGTGCTGCTG TAATCCAGAA AGCCAGACCC
    ACCAAGAAAC AGAAGCACCA GCCAGGACAT CTGCGCAGAG AGGCCTACAC AGATGATCTT
    CCACCCCCTC CAGTGCCACC ACCTGCTATA AAATCGCCCA GTGTCCAGTC CAAGGCACAG
    CTGGAGGCAC GGCCTATAAT GGGGCCAAAG CTCGCTTCTA TAGAAGCGAG AGCAGACAGA
    TCATCAGACA GAAAAGGCGG CAGTTACAAG GGGAGAGAAG CTCTGGACGG AAGACAAGTC
    ACTGACCTGC GAACAAGCCC AGGTGACCCC AGAGAAGCAC AGGAGCAGCC AAACGAAGGG
    AAAGCACGAG GGACGAAGAC AGCAAAGCGA GACCTTCCAC CAGCGAAGAC ACACCTCATC
    CCAGAGGATA TCCTTCCGTA CTGTAGGCCT ACTTTCCCAA CGTCAAACAA CCCTAGGGAT
    CCCAGTTCTT CAAGCTCAAT GTCATCAAGA GGCTCAGGAA GCCGACAGAG AGAGCAAGCA
    AATGTGGGCC GAAGAAATAT GGCAGAAATG CAAGTACTTG GGGGATTTGA AAGAGGAGAT
    GAGAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453549.1
    CDS371..5326
    Translation

    Target ORF information:

    RefSeq Version XM_017598060.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598060.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATCTTCC TCTTCTGGTC ATGATATCTC TCCTCACTCT ATCAAAAAAG 
    CATCTTCTTC TGGCCCAGCT TATTCCAGAC CCTGAGGACC TAGAGAGGGG GAACGACAAC
    GGGACGCCAG CCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTCCGTCAGG AAGATTTCCC ACCGCGCATT GTTGAGCACC CTTCAGACCT GATCGTCTCT
    AAAGGGGAAC CCGCCACCCT CAACTGTAAA GCTGAAGGCC GCCCTACCCC GACTATAGAA
    TGGTACAAGG GCGGCGAAAG AGTGGAAACG GACAAAGACG ACCCTCGCTC ACACCGAATG
    CTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGCATAGTGC ATGGACGGAA GAGTAGGCCT
    GACGAAGGGG TCTACATCTG TGTAGCAAGG AATTACCTCG GAGAGGCTGT GAGCCACAAT
    GCATCGCTGG AGGTAGCTAT ACTACGGGAT GACTTCAGAC AAAACCCTTC AGATGTTATG
    GTGGCGGTGG GCGAGCCTGC AGTAATGGAA TGCCAGCCTC CTCGGGGCCA CCCAGAGCCC
    ACCATCTCCT GGAAAAAGGA TGGCTCACCC CTGGATGATA AAGACGAGCG AATCACTATC
    CGAGGCGGGA AGCTCATGAT CACGTACACC CGTAAAAGCG ATGCTGGCAA GTATGTTTGT
    GTTGGTACCA ACATGGTAGG GGAGCGGGAG AGCGAAGTCG CGGAGCTGAC TGTCTTAGAG
    AGACCGTCCT TTGTAAAGAG ACCCAGTAAT TTGGCAGTGA CTGTGGATGA CAGTGCTGAG
    TTTAAGTGTG AGGCCCGAGG AGACCCCGTG CCTACGGTTC GGTGGAGGAA AGATGACGGA
    GAGCTGCCCA AATCCAGATA TGAGATCCGA GATGATCATA CTTTGAAAAT TCGGAAGGTG
    ACAGCTGGCG ACATGGGATC ATACACTTGT GTTGCAGAAA ATATGGTTGG GAAAGCCGAA
    GCATCAGCCA CTTTGACAGT TCAAGAGCCA CCACATTTTG TCGTGAAGCC TCGAGACCAG
    GTGGTTGCCT TGGGGCGAAC TGTGACTTTT CAGTGTGAAG CAACTGGAAA TCCTCAACCA
    GCTATCTTTT GGCGGAGAGA GGGGAGTCAG AATCTGCTTT TCTCATACCA GCCTCCACAG
    TCCTCTAGCC GGTTTTCAGT TTCCCAGACG GGGGACCTCA CTGTTACGAA CGTCCAGAGG
    TCTGATGTCG GTTACTATAT CTGTCAGACT TTGAACGTGG CTGGAAGCAT CATCACAAAG
    GCATATTTGG AAGTTACTGA TGTGATTGCG GACCGGCCTC CCCCAGTCAT CCGACAAGGT
    CCTGTGAATC AGACTGTAGC AGTGGATGGT ACTTTAACTC TCAGCTGTGT GGCCACAGGC
    AGTCCCGTGC CCACAATTCT GTGGAGGAAG GATGGAGTCC TTGTTTCAAC CCAAGATTCT
    CGGATCAAAC AGCTGGAGAG CGGGGTCCTG CAGATCCGCT ACGCTAAGCT GGGTGACACC
    GGTCGGTACA CCTGCACTGC TTCGACCCCC AGTGGTGAGG CCACGTGGAG CGCTTACATC
    GAAGTCCAGG AATTTGGAGT TCCAGTTCAA CCTCCAAGAC CCACCGACCC CAATTTAATC
    CCCAGTGCGC CTTCAAAGCC TGAAGTCACA GATGTGAGCA AAAACACAGT CACTTTATTG
    TGGCAGCCTA ACCTGAACTC AGGAGCAACC CCAACGTCTT ATATTATAGA AGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAGACCGTG GCAGAAAATG TGAAAACAGA AACATTCGCC
    ATCAAAGGGC TCAAACCTAA TGCGATCTAC CTTTTCCTGG TGAGGGCAGC AAATGCCTAT
    GGGATTAGCG ATCCAAGCCA AATATCCGAT CCAGTGAAAA CACAAGATGT CCCACCAACC
    ACCCAAGGGG TGGACCACAA GCAGGTCCAG AGAGAACTGG GCAATGTTGT CTTGCATCTC
    CACAACCCTA CCATCCTTTC TTCCTCTTCT GTCGAAGTGC ACTGGACGGT GGACCAACAA
    TCTCAATATA TTCAAGGTTA TAAAATTCTC TACCGACCAT CTGGAGCCAG CCATGGCGAA
    TCGGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ATAGTGTGGT GATCCCCGAT
    CTGAGAAAAG GCGTCAACTA CGAGATTAAG GCCCGCCCAT TTTTTAATGA GTTCCAAGGA
    GCAGATAGTG AAATCAAATT TGCCAAAACC TTAGAAGAAG CACCAAGTGC CCCTCCCCGA
    AGTGTCACTG TATCCAAGAA TGATGGAAAT GGGACAGCAA TTCTGGTCAC TTGGCAACCG
    CCTCCTGAAG ACACACAGAA TGGAATGGTC CAAGAATATA AGGTTTGGTG TCTCGGGAAT
    GAGACTAGGT ATCACATAAA CAAGACAGTA GATGGCTCTA CCTTTTCGGT GGTCATCCCC
    TCTCTGGTTC CTGGGATCCG CTACAGTGTA GAAGTGGCTG CAAGCACTGG GGCTGGCCCT
    GGGGTGAAGA GTGAACCCCA GTTCATCCAG TTAGATTCCC ATGGAAACCC CGTGTCTCCC
    GAGGACCAAG TGAGCCTGGC TCAGCAGATC TCCGACGTGG TGAAGCAGCC AGCCTTCATT
    GCGGGCATCG GGGCCGCCTG TTGGATCATT CTCATGGTCT TCAGTATCTG GCTTTACCGA
    CACCGCAAGA AGAGAAACGG GCTCAGCAGC ACGTACGCGG GGATCCGGAA AGTCCCGTCC
    TTTACCTTCA CACCAACAGT AACTTATCAA AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG
    AGGCCGGGAC TCCTCAACAT CAGTGAACCT GCCACACAGC CATGGCTGGC GGACACGTGG
    CCCAACACAG GCAACAGTCA CAATGACTGC TCCATCAACT GCTGCACAGC CAGCAACGGC
    AACAGCGACA GCAACCTGAC CACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC
    AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT
    GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAC
    GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCCTACG CCACCACGCA GCTCATCCAG
    GCAAATCTCA GCAACAATAT GAATAACGGC GGCGGGGACT CCAGCGAGAA ACACTGGAAG
    CCTCCGGGGC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAGCTGAACA AAGATTATCG GGCAAATGAC ACAATCCCTC CAACCATCCC GTACAACCAA
    TCCTATGACC AGAATACAGG AGGATCTTAC AACAGCTCAG ATCGGGGCAG TAGTACATCT
    GGGAGTCAAG GCCACAAGAA AGGGGCACGG ACACCAAAGG CACCCAAGCA GGGTGGCATG
    AACTGGGCAG ACTTGCTGCC CCCTCCCCCA GCGCATCCTC CCCCACACAG CAACAGCGAG
    GAATACAGCA TGTCTGTAGA TGAAAGCTAT GATCAAGAAA TGCCGTGCCC TGTTCCGCCT
    GCACGGATGT ATTTGCAACA GGATGAATTA GAAGAAGAGG AAGCTGAAAG AGGCCCCACG
    CCCCCTGTCC GGGGGGCAGC TTCGTCCCCA GCTGCTGTGT CCTACAGCCA TCAGTCCACG
    GCCACTCTCA CCCCGTCTCC CCAGGAAGAG CTCCAGCCTA TGTTACAGGA TTGTCCGGAG
    GACCTCGGCC ACATGCCCCA CCCACCAGAC AGGAGGCGGC AGCCGGTGAG TCCTCCTCCA
    CCACCACGGC CAATTTCCCC ACCACATACT TACGGCTACA TTTCAGGACC CCTGGTCTCA
    GACATGGATA CGGATGCCCC AGAAGAGGAG GAAGACGAAG CTGACATGGA AGTTGCTAAG
    ATGCAGACCC GAAGGCTGTT GTTACGTGGG CTTGAACAGA CCCCAGCATC TAGTGTTGGG
    GACCTCGAGA GCTCGGTCAC TGGCTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAAGAG
    GACAACATTT CCAGTGGGCG CTCCAGTGTC AGTTCATCGG ATGGTTCCTT TTTCACTGAC
    GCTGATTTTG CCCAGGCAGT TGCCGCAGCC GCCGAGTATG CGGGCCTGAA GGTGGCTCGC
    CGCCAGATGC AGGATGCTGC TGGCCGCCGA CACTTCCATG CCTCCCAGTG CCCTAGGCCC
    ACGAGTCCTG TGTCTACAGA CAGCAACATG AGTGCTGCTG TAATCCAGAA AGCCAGACCC
    ACCAAGAAAC AGAAGCACCA GCCAGGACAT CTGCGCAGAG AGGCCTACAC AGATGATCTT
    CCACCCCCTC CAGTGCCACC ACCTGCTATA AAATCGCCCA GTGTCCAGTC CAAGGCACAG
    CTGGAGGCAC GGCCTATAAT GGGGCCAAAG CTCGCTTCTA TAGAAGCGAG AGCAGACAGA
    TCATCAGACA GAAAAGGCGG CAGTTACAAG GGGAGAGAAG CTCTGGACGG AAGACAAGTC
    ACTGACCTGC GAACAAGCCC AGGTGACCCC AGAGAAGCAC AGGAGCAGCC AAACGAAGGG
    AAAGCACGAG GGACGAAGAC AGCAAAGCGA GACCTTCCAC CAGCGAAGAC ACACCTCATC
    CCAGAGGATA TCCTTCCGTA CTGTAGGCCT ACTTTCCCAA CGTCAAACAA CCCTAGGGAT
    CCCAGTTCTT CAAGCTCAAT GTCATCAAGA GGCTCAGGAA GCCGACAGAG AGAGCAAGCA
    AATGTGGGCC GAAGAAATAT GGCAGAAATG CAAGTACTTG GGGGATTTGA AAGAGGAGAT
    GAGAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57713
    Clone ID Related Accession (Same CDS sequence) XM_017598061.1
    Accession Version XM_017598061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTTACTGAT ACTGCTCTTC TCAATGGCTG AAATAACTTC TCCTGTGGGT 
    GTCTCCGGCA GAATGAAAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTC
    CGTCAGGAAG ATTTCCCACC GCGCATTGTT GAGCACCCTT CAGACCTGAT CGTCTCTAAA
    GGGGAACCCG CCACCCTCAA CTGTAAAGCT GAAGGCCGCC CTACCCCGAC TATAGAATGG
    TACAAGGGCG GCGAAAGAGT GGAAACGGAC AAAGACGACC CTCGCTCACA CCGAATGCTG
    CTGCCAAGTG GATCTTTATT TTTCTTACGC ATAGTGCATG GACGGAAGAG TAGGCCTGAC
    GAAGGGGTCT ACATCTGTGT AGCAAGGAAT TACCTCGGAG AGGCTGTGAG CCACAATGCA
    TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTTATGGTG
    GCGGTGGGCG AGCCTGCAGT AATGGAATGC CAGCCTCCTC GGGGCCACCC AGAGCCCACC
    ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ACGAGCGAAT CACTATCCGA
    GGCGGGAAGC TCATGATCAC GTACACCCGT AAAAGCGATG CTGGCAAGTA TGTTTGTGTT
    GGTACCAACA TGGTAGGGGA GCGGGAGAGC GAAGTCGCGG AGCTGACTGT CTTAGAGAGA
    CCGTCCTTTG TAAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCTGAGTTT
    AAGTGTGAGG CCCGAGGAGA CCCCGTGCCT ACGGTTCGGT GGAGGAAAGA TGACGGAGAG
    CTGCCCAAAT CCAGATATGA GATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACA
    GCTGGCGACA TGGGATCATA CACTTGTGTT GCAGAAAATA TGGTTGGGAA AGCCGAAGCA
    TCAGCCACTT TGACAGTTCA AGTTGGGTCT GAGCCACCAC ATTTTGTCGT GAAGCCTCGA
    GACCAGGTGG TTGCCTTGGG GCGAACTGTG ACTTTTCAGT GTGAAGCAAC TGGAAATCCT
    CAACCAGCTA TCTTTTGGCG GAGAGAGGGG AGTCAGAATC TGCTTTTCTC ATACCAGCCT
    CCACAGTCCT CTAGCCGGTT TTCAGTTTCC CAGACGGGGG ACCTCACTGT TACGAACGTC
    CAGAGGTCTG ATGTCGGTTA CTATATCTGT CAGACTTTGA ACGTGGCTGG AAGCATCATC
    ACAAAGGCAT ATTTGGAAGT TACTGATGTG ATTGCGGACC GGCCTCCCCC AGTCATCCGA
    CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGTACTT TAACTCTCAG CTGTGTGGCC
    ACAGGCAGTC CCGTGCCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA
    GATTCTCGGA TCAAACAGCT GGAGAGCGGG GTCCTGCAGA TCCGCTACGC TAAGCTGGGT
    GACACCGGTC GGTACACCTG CACTGCTTCG ACCCCCAGTG GTGAGGCCAC GTGGAGCGCT
    TACATCGAAG TCCAGGAATT TGGAGTTCCA GTTCAACCTC CAAGACCCAC CGACCCCAAT
    TTAATCCCCA GTGCGCCTTC AAAGCCTGAA GTCACAGATG TGAGCAAAAA CACAGTCACT
    TTATTGTGGC AGCCTAACCT GAACTCAGGA GCAACCCCAA CGTCTTATAT TATAGAAGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACCGTGGCAG AAAATGTGAA AACAGAAACA
    TTCGCCATCA AAGGGCTCAA ACCTAATGCG ATCTACCTTT TCCTGGTGAG GGCAGCAAAT
    GCCTATGGGA TTAGCGATCC AAGCCAAATA TCCGATCCAG TGAAAACACA AGATGTCCCA
    CCAACCACCC AAGGGGTGGA CCACAAGCAG GTCCAGAGAG AACTGGGCAA TGTTGTCTTG
    CATCTCCACA ACCCTACCAT CCTTTCTTCC TCTTCTGTCG AAGTGCACTG GACGGTGGAC
    CAACAATCTC AATATATTCA AGGTTATAAA ATTCTCTACC GACCATCTGG AGCCAGCCAT
    GGCGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACCCCAA CCAAAAATAG TGTGGTGATC
    CCCGATCTGA GAAAAGGCGT CAACTACGAG ATTAAGGCCC GCCCATTTTT TAATGAGTTC
    CAAGGAGCAG ATAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT
    CCCCGAAGTG TCACTGTATC CAAGAATGAT GGAAATGGGA CAGCAATTCT GGTCACTTGG
    CAACCGCCTC CTGAAGACAC ACAGAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC
    GGGAATGAGA CTAGGTATCA CATAAACAAG ACAGTAGATG GCTCTACCTT TTCGGTGGTC
    ATCCCCTCTC TGGTTCCTGG GATCCGCTAC AGTGTAGAAG TGGCTGCAAG CACTGGGGCT
    GGCCCTGGGG TGAAGAGTGA ACCCCAGTTC ATCCAGTTAG ATTCCCATGG AAACCCCGTG
    TCTCCCGAGG ACCAAGTGAG CCTGGCTCAG CAGATCTCCG ACGTGGTGAA GCAGCCAGCC
    TTCATTGCGG GCATCGGGGC CGCCTGTTGG ATCATTCTCA TGGTCTTCAG TATCTGGCTT
    TACCGACACC GCAAGAAGAG AAACGGGCTC AGCAGCACGT ACGCGGGGAT CCGGAAAGTC
    CCGTCCTTTA CCTTCACACC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT
    GGAGGGAGGC CGGGACTCCT CAACATCAGT GAACCTGCCA CACAGCCATG GCTGGCGGAC
    ACGTGGCCCA ACACAGGCAA CAGTCACAAT GACTGCTCCA TCAACTGCTG CACAGCCAGC
    AACGGCAACA GCGACAGCAA CCTGACCACC TACAGTCGCC CAGCTGATTG TATAGCAAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTACGCCAC CACGCAGCTC
    ATCCAGGCAA ATCTCAGCAA CAATATGAAT AACGGCGGCG GGGACTCCAG CGAGAAACAC
    TGGAAGCCTC CGGGGCAGCA GAAACAGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG
    CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACAA TCCCTCCAAC CATCCCGTAC
    AACCAATCCT ATGACCAGAA TACAGGAGGA TCTTACAACA GCTCAGATCG GGGCAGTAGT
    ACATCTGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT
    GGCATGAACT GGGCAGACTT GCTGCCCCCT CCCCCAGCGC ATCCTCCCCC ACACAGCAAC
    AGCGAGGAAT ACAGCATGTC TGTAGATGAA AGCTATGATC AAGAAATGCC GTGCCCTGTT
    CCGCCTGCAC GGATGTATTT GCAACAGGAT GAATTAGAAG AAGAGGAAGC TGAAAGAGGC
    CCCACGCCCC CTGTCCGGGG GGCAGCTTCG TCCCCAGCTG CTGTGTCCTA CAGCCATCAG
    TCCACGGCCA CTCTCACCCC GTCTCCCCAG GAAGAGCTCC AGCCTATGTT ACAGGATTGT
    CCGGAGGACC TCGGCCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC GGTGAGTCCT
    CCTCCACCAC CACGGCCAAT TTCCCCACCA CATACTTACG GCTACATTTC AGGACCCCTG
    GTCTCAGACA TGGATACGGA TGCCCCAGAA GAGGAGGAAG ACGAAGCTGA CATGGAAGTT
    GCTAAGATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTTG AACAGACCCC AGCATCTAGT
    GTTGGGGACC TCGAGAGCTC GGTCACTGGC TCCATGATCA ATGGCTGGGG CTCAGCCTCA
    GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCGGATGG TTCCTTTTTC
    ACTGACGCTG ATTTTGCCCA GGCAGTTGCC GCAGCCGCCG AGTATGCGGG CCTGAAGGTG
    GCTCGCCGCC AGATGCAGGA TGCTGCTGGC CGCCGACACT TCCATGCCTC CCAGTGCCCT
    AGGCCCACGA GTCCTGTGTC TACAGACAGC AACATGAGTG CTGCTGTAAT CCAGAAAGCC
    AGACCCACCA AGAAACAGAA GCACCAGCCA GGACATCTGC GCAGAGAGGC CTACACAGAT
    GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCAGTGT CCAGTCCAAG
    GCACAGCTGG AGGCACGGCC TATAATGGGG CCAAAGCTCG CTTCTATAGA AGCGAGAGCA
    GACAGATCAT CAGACAGAAA AGGCGGCAGT TACAAGGGGA GAGAAGCTCT GGACGGAAGA
    CAAGTCACTG ACCTGCGAAC AAGCCCAGGT GACCCCAGAG AAGCACAGGA GCAGCCAAAC
    GAAGGGAAAG CACGAGGGAC GAAGACAGCA AAGCGAGACC TTCCACCAGC GAAGACACAC
    CTCATCCCAG AGGATATCCT TCCGTACTGT AGGCCTACTT TCCCAACGTC AAACAACCCT
    AGGGATCCCA GTTCTTCAAG CTCAATGTCA TCAAGAGGCT CAGGAAGCCG ACAGAGAGAG
    CAAGCAAATG TGGGCCGAAG AAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA
    GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453550.1
    CDS1645..6546
    Translation

    Target ORF information:

    RefSeq Version XM_017598061.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTTACTGAT ACTGCTCTTC TCAATGGCTG AAATAACTTC TCCTGTGGGT 
    GTCTCCGGCA GAATGAAAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTC
    CGTCAGGAAG ATTTCCCACC GCGCATTGTT GAGCACCCTT CAGACCTGAT CGTCTCTAAA
    GGGGAACCCG CCACCCTCAA CTGTAAAGCT GAAGGCCGCC CTACCCCGAC TATAGAATGG
    TACAAGGGCG GCGAAAGAGT GGAAACGGAC AAAGACGACC CTCGCTCACA CCGAATGCTG
    CTGCCAAGTG GATCTTTATT TTTCTTACGC ATAGTGCATG GACGGAAGAG TAGGCCTGAC
    GAAGGGGTCT ACATCTGTGT AGCAAGGAAT TACCTCGGAG AGGCTGTGAG CCACAATGCA
    TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTTATGGTG
    GCGGTGGGCG AGCCTGCAGT AATGGAATGC CAGCCTCCTC GGGGCCACCC AGAGCCCACC
    ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ACGAGCGAAT CACTATCCGA
    GGCGGGAAGC TCATGATCAC GTACACCCGT AAAAGCGATG CTGGCAAGTA TGTTTGTGTT
    GGTACCAACA TGGTAGGGGA GCGGGAGAGC GAAGTCGCGG AGCTGACTGT CTTAGAGAGA
    CCGTCCTTTG TAAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCTGAGTTT
    AAGTGTGAGG CCCGAGGAGA CCCCGTGCCT ACGGTTCGGT GGAGGAAAGA TGACGGAGAG
    CTGCCCAAAT CCAGATATGA GATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACA
    GCTGGCGACA TGGGATCATA CACTTGTGTT GCAGAAAATA TGGTTGGGAA AGCCGAAGCA
    TCAGCCACTT TGACAGTTCA AGTTGGGTCT GAGCCACCAC ATTTTGTCGT GAAGCCTCGA
    GACCAGGTGG TTGCCTTGGG GCGAACTGTG ACTTTTCAGT GTGAAGCAAC TGGAAATCCT
    CAACCAGCTA TCTTTTGGCG GAGAGAGGGG AGTCAGAATC TGCTTTTCTC ATACCAGCCT
    CCACAGTCCT CTAGCCGGTT TTCAGTTTCC CAGACGGGGG ACCTCACTGT TACGAACGTC
    CAGAGGTCTG ATGTCGGTTA CTATATCTGT CAGACTTTGA ACGTGGCTGG AAGCATCATC
    ACAAAGGCAT ATTTGGAAGT TACTGATGTG ATTGCGGACC GGCCTCCCCC AGTCATCCGA
    CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGTACTT TAACTCTCAG CTGTGTGGCC
    ACAGGCAGTC CCGTGCCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA
    GATTCTCGGA TCAAACAGCT GGAGAGCGGG GTCCTGCAGA TCCGCTACGC TAAGCTGGGT
    GACACCGGTC GGTACACCTG CACTGCTTCG ACCCCCAGTG GTGAGGCCAC GTGGAGCGCT
    TACATCGAAG TCCAGGAATT TGGAGTTCCA GTTCAACCTC CAAGACCCAC CGACCCCAAT
    TTAATCCCCA GTGCGCCTTC AAAGCCTGAA GTCACAGATG TGAGCAAAAA CACAGTCACT
    TTATTGTGGC AGCCTAACCT GAACTCAGGA GCAACCCCAA CGTCTTATAT TATAGAAGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACCGTGGCAG AAAATGTGAA AACAGAAACA
    TTCGCCATCA AAGGGCTCAA ACCTAATGCG ATCTACCTTT TCCTGGTGAG GGCAGCAAAT
    GCCTATGGGA TTAGCGATCC AAGCCAAATA TCCGATCCAG TGAAAACACA AGATGTCCCA
    CCAACCACCC AAGGGGTGGA CCACAAGCAG GTCCAGAGAG AACTGGGCAA TGTTGTCTTG
    CATCTCCACA ACCCTACCAT CCTTTCTTCC TCTTCTGTCG AAGTGCACTG GACGGTGGAC
    CAACAATCTC AATATATTCA AGGTTATAAA ATTCTCTACC GACCATCTGG AGCCAGCCAT
    GGCGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACCCCAA CCAAAAATAG TGTGGTGATC
    CCCGATCTGA GAAAAGGCGT CAACTACGAG ATTAAGGCCC GCCCATTTTT TAATGAGTTC
    CAAGGAGCAG ATAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT
    CCCCGAAGTG TCACTGTATC CAAGAATGAT GGAAATGGGA CAGCAATTCT GGTCACTTGG
    CAACCGCCTC CTGAAGACAC ACAGAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC
    GGGAATGAGA CTAGGTATCA CATAAACAAG ACAGTAGATG GCTCTACCTT TTCGGTGGTC
    ATCCCCTCTC TGGTTCCTGG GATCCGCTAC AGTGTAGAAG TGGCTGCAAG CACTGGGGCT
    GGCCCTGGGG TGAAGAGTGA ACCCCAGTTC ATCCAGTTAG ATTCCCATGG AAACCCCGTG
    TCTCCCGAGG ACCAAGTGAG CCTGGCTCAG CAGATCTCCG ACGTGGTGAA GCAGCCAGCC
    TTCATTGCGG GCATCGGGGC CGCCTGTTGG ATCATTCTCA TGGTCTTCAG TATCTGGCTT
    TACCGACACC GCAAGAAGAG AAACGGGCTC AGCAGCACGT ACGCGGGGAT CCGGAAAGTC
    CCGTCCTTTA CCTTCACACC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT
    GGAGGGAGGC CGGGACTCCT CAACATCAGT GAACCTGCCA CACAGCCATG GCTGGCGGAC
    ACGTGGCCCA ACACAGGCAA CAGTCACAAT GACTGCTCCA TCAACTGCTG CACAGCCAGC
    AACGGCAACA GCGACAGCAA CCTGACCACC TACAGTCGCC CAGCTGATTG TATAGCAAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTACGCCAC CACGCAGCTC
    ATCCAGGCAA ATCTCAGCAA CAATATGAAT AACGGCGGCG GGGACTCCAG CGAGAAACAC
    TGGAAGCCTC CGGGGCAGCA GAAACAGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG
    CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACAA TCCCTCCAAC CATCCCGTAC
    AACCAATCCT ATGACCAGAA TACAGGAGGA TCTTACAACA GCTCAGATCG GGGCAGTAGT
    ACATCTGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT
    GGCATGAACT GGGCAGACTT GCTGCCCCCT CCCCCAGCGC ATCCTCCCCC ACACAGCAAC
    AGCGAGGAAT ACAGCATGTC TGTAGATGAA AGCTATGATC AAGAAATGCC GTGCCCTGTT
    CCGCCTGCAC GGATGTATTT GCAACAGGAT GAATTAGAAG AAGAGGAAGC TGAAAGAGGC
    CCCACGCCCC CTGTCCGGGG GGCAGCTTCG TCCCCAGCTG CTGTGTCCTA CAGCCATCAG
    TCCACGGCCA CTCTCACCCC GTCTCCCCAG GAAGAGCTCC AGCCTATGTT ACAGGATTGT
    CCGGAGGACC TCGGCCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC GGTGAGTCCT
    CCTCCACCAC CACGGCCAAT TTCCCCACCA CATACTTACG GCTACATTTC AGGACCCCTG
    GTCTCAGACA TGGATACGGA TGCCCCAGAA GAGGAGGAAG ACGAAGCTGA CATGGAAGTT
    GCTAAGATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTTG AACAGACCCC AGCATCTAGT
    GTTGGGGACC TCGAGAGCTC GGTCACTGGC TCCATGATCA ATGGCTGGGG CTCAGCCTCA
    GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCGGATGG TTCCTTTTTC
    ACTGACGCTG ATTTTGCCCA GGCAGTTGCC GCAGCCGCCG AGTATGCGGG CCTGAAGGTG
    GCTCGCCGCC AGATGCAGGA TGCTGCTGGC CGCCGACACT TCCATGCCTC CCAGTGCCCT
    AGGCCCACGA GTCCTGTGTC TACAGACAGC AACATGAGTG CTGCTGTAAT CCAGAAAGCC
    AGACCCACCA AGAAACAGAA GCACCAGCCA GGACATCTGC GCAGAGAGGC CTACACAGAT
    GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCAGTGT CCAGTCCAAG
    GCACAGCTGG AGGCACGGCC TATAATGGGG CCAAAGCTCG CTTCTATAGA AGCGAGAGCA
    GACAGATCAT CAGACAGAAA AGGCGGCAGT TACAAGGGGA GAGAAGCTCT GGACGGAAGA
    CAAGTCACTG ACCTGCGAAC AAGCCCAGGT GACCCCAGAG AAGCACAGGA GCAGCCAAAC
    GAAGGGAAAG CACGAGGGAC GAAGACAGCA AAGCGAGACC TTCCACCAGC GAAGACACAC
    CTCATCCCAG AGGATATCCT TCCGTACTGT AGGCCTACTT TCCCAACGTC AAACAACCCT
    AGGGATCCCA GTTCTTCAAG CTCAATGTCA TCAAGAGGCT CAGGAAGCCG ACAGAGAGAG
    CAAGCAAATG TGGGCCGAAG AAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA
    GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57714
    Clone ID Related Accession (Same CDS sequence) XM_017598062.1
    Accession Version XM_017598062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCACC ACATTTTGTC GTGAAGCCTC GAGACCAGGT GGTTGCCTTG
    GGGCGAACTG TGACTTTTCA GTGTGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    CGGAGAGAGG GGAGTCAGAA TCTGCTTTTC TCATACCAGC CTCCACAGTC CTCTAGCCGG
    TTTTCAGTTT CCCAGACGGG GGACCTCACT GTTACGAACG TCCAGAGGTC TGATGTCGGT
    TACTATATCT GTCAGACTTT GAACGTGGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA
    GTTACTGATG TGATTGCGGA CCGGCCTCCC CCAGTCATCC GACAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGTAC TTTAACTCTC AGCTGTGTGG CCACAGGCAG TCCCGTGCCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGCG GGGTCCTGCA GATCCGCTAC GCTAAGCTGG GTGACACCGG TCGGTACACC
    TGCACTGCTT CGACCCCCAG TGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGAGTTC CAGTTCAACC TCCAAGACCC ACCGACCCCA ATTTAATCCC CAGTGCGCCT
    TCAAAGCCTG AAGTCACAGA TGTGAGCAAA AACACAGTCA CTTTATTGTG GCAGCCTAAC
    CTGAACTCAG GAGCAACCCC AACGTCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACCGTGGC AGAAAATGTG AAAACAGAAA CATTCGCCAT CAAAGGGCTC
    AAACCTAATG CGATCTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG GATTAGCGAT
    CCAAGCCAAA TATCCGATCC AGTGAAAACA CAAGATGTCC CACCAACCAC CCAAGGGGTG
    GACCACAAGC AGGTCCAGAG AGAACTGGGC AATGTTGTCT TGCATCTCCA CAACCCTACC
    ATCCTTTCTT CCTCTTCTGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGTTATA AAATTCTCTA CCGACCATCT GGAGCCAGCC ATGGCGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACCCC AACCAAAAAT AGTGTGGTGA TCCCCGATCT GAGAAAAGGC
    GTCAACTACG AGATTAAGGC CCGCCCATTT TTTAATGAGT TCCAAGGAGC AGATAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTCACTGTA
    TCCAAGAATG ATGGAAATGG GACAGCAATT CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAGAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGGAATGA GACTAGGTAT
    CACATAAACA AGACAGTAGA TGGCTCTACC TTTTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGCT ACAGTGTAGA AGTGGCTGCA AGCACTGGGG CTGGCCCTGG GGTGAAGAGT
    GAACCCCAGT TCATCCAGTT AGATTCCCAT GGAAACCCCG TGTCTCCCGA GGACCAAGTG
    AGCCTGGCTC AGCAGATCTC CGACGTGGTG AAGCAGCCAG CCTTCATTGC GGGCATCGGG
    GCCGCCTGTT GGATCATTCT CATGGTCTTC AGTATCTGGC TTTACCGACA CCGCAAGAAG
    AGAAACGGGC TCAGCAGCAC GTACGCGGGG ATCCGGAAAG TCCCGTCCTT TACCTTCACA
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATCA GTGAACCTGC CACACAGCCA TGGCTGGCGG ACACGTGGCC CAACACAGGC
    AACAGTCACA ATGACTGCTC CATCAACTGC TGCACAGCCA GCAACGGCAA CAGCGACAGC
    AACCTGACCA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ACGCCACCAC GCAGCTCATC
    CAGGCAAATC TCAGCAACAA TATGAATAAC GGCGGCGGGG ACTCCAGCGA GAAACACTGG
    AAGCCTCCGG GGCAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACAATCC CTCCAACCAT CCCGTACAAC
    CAATCCTATG ACCAGAATAC AGGAGGATCT TACAACAGCT CAGATCGGGG CAGTAGTACA
    TCTGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCC CCAGCGCATC CTCCCCCACA CAGCAACAGC
    GAGGAATACA GCATGTCTGT AGATGAAAGC TATGATCAAG AAATGCCGTG CCCTGTTCCG
    CCTGCACGGA TGTATTTGCA ACAGGATGAA TTAGAAGAAG AGGAAGCTGA AAGAGGCCCC
    ACGCCCCCTG TCCGGGGGGC AGCTTCGTCC CCAGCTGCTG TGTCCTACAG CCATCAGTCC
    ACGGCCACTC TCACCCCGTC TCCCCAGGAA GAGCTCCAGC CTATGTTACA GGATTGTCCG
    GAGGACCTCG GCCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCGGT GAGTCCTCCT
    CCACCACCAC GGCCAATTTC CCCACCACAT ACTTACGGCT ACATTTCAGG ACCCCTGGTC
    TCAGACATGG ATACGGATGC CCCAGAAGAG GAGGAAGACG AAGCTGACAT GGAAGTTGCT
    AAGATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTTGAAC AGACCCCAGC ATCTAGTGTT
    GGGGACCTCG AGAGCTCGGT CACTGGCTCC ATGATCAATG GCTGGGGCTC AGCCTCAGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CGGATGGTTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCCGCA GCCGCCGAGT ATGCGGGCCT GAAGGTGGCT
    CGCCGCCAGA TGCAGGATGC TGCTGGCCGC CGACACTTCC ATGCCTCCCA GTGCCCTAGG
    CCCACGAGTC CTGTGTCTAC AGACAGCAAC ATGAGTGCTG CTGTAATCCA GAAAGCCAGA
    CCCACCAAGA AACAGAAGCA CCAGCCAGGA CATCTGCGCA GAGAGGCCTA CACAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCAGTGTCCA GTCCAAGGCA
    CAGCTGGAGG CACGGCCTAT AATGGGGCCA AAGCTCGCTT CTATAGAAGC GAGAGCAGAC
    AGATCATCAG ACAGAAAAGG CGGCAGTTAC AAGGGGAGAG AAGCTCTGGA CGGAAGACAA
    GTCACTGACC TGCGAACAAG CCCAGGTGAC CCCAGAGAAG CACAGGAGCA GCCAAACGAA
    GGGAAAGCAC GAGGGACGAA GACAGCAAAG CGAGACCTTC CACCAGCGAA GACACACCTC
    ATCCCAGAGG ATATCCTTCC GTACTGTAGG CCTACTTTCC CAACGTCAAA CAACCCTAGG
    GATCCCAGTT CTTCAAGCTC AATGTCATCA AGAGGCTCAG GAAGCCGACA GAGAGAGCAA
    GCAAATGTGG GCCGAAGAAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453551.1
    CDS241..5139
    Translation

    Target ORF information:

    RefSeq Version XM_017598062.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCACC ACATTTTGTC GTGAAGCCTC GAGACCAGGT GGTTGCCTTG
    GGGCGAACTG TGACTTTTCA GTGTGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    CGGAGAGAGG GGAGTCAGAA TCTGCTTTTC TCATACCAGC CTCCACAGTC CTCTAGCCGG
    TTTTCAGTTT CCCAGACGGG GGACCTCACT GTTACGAACG TCCAGAGGTC TGATGTCGGT
    TACTATATCT GTCAGACTTT GAACGTGGCT GGAAGCATCA TCACAAAGGC ATATTTGGAA
    GTTACTGATG TGATTGCGGA CCGGCCTCCC CCAGTCATCC GACAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGTAC TTTAACTCTC AGCTGTGTGG CCACAGGCAG TCCCGTGCCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGCG GGGTCCTGCA GATCCGCTAC GCTAAGCTGG GTGACACCGG TCGGTACACC
    TGCACTGCTT CGACCCCCAG TGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGAGTTC CAGTTCAACC TCCAAGACCC ACCGACCCCA ATTTAATCCC CAGTGCGCCT
    TCAAAGCCTG AAGTCACAGA TGTGAGCAAA AACACAGTCA CTTTATTGTG GCAGCCTAAC
    CTGAACTCAG GAGCAACCCC AACGTCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACCGTGGC AGAAAATGTG AAAACAGAAA CATTCGCCAT CAAAGGGCTC
    AAACCTAATG CGATCTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG GATTAGCGAT
    CCAAGCCAAA TATCCGATCC AGTGAAAACA CAAGATGTCC CACCAACCAC CCAAGGGGTG
    GACCACAAGC AGGTCCAGAG AGAACTGGGC AATGTTGTCT TGCATCTCCA CAACCCTACC
    ATCCTTTCTT CCTCTTCTGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGTTATA AAATTCTCTA CCGACCATCT GGAGCCAGCC ATGGCGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACCCC AACCAAAAAT AGTGTGGTGA TCCCCGATCT GAGAAAAGGC
    GTCAACTACG AGATTAAGGC CCGCCCATTT TTTAATGAGT TCCAAGGAGC AGATAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTCACTGTA
    TCCAAGAATG ATGGAAATGG GACAGCAATT CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAGAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGGAATGA GACTAGGTAT
    CACATAAACA AGACAGTAGA TGGCTCTACC TTTTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGCT ACAGTGTAGA AGTGGCTGCA AGCACTGGGG CTGGCCCTGG GGTGAAGAGT
    GAACCCCAGT TCATCCAGTT AGATTCCCAT GGAAACCCCG TGTCTCCCGA GGACCAAGTG
    AGCCTGGCTC AGCAGATCTC CGACGTGGTG AAGCAGCCAG CCTTCATTGC GGGCATCGGG
    GCCGCCTGTT GGATCATTCT CATGGTCTTC AGTATCTGGC TTTACCGACA CCGCAAGAAG
    AGAAACGGGC TCAGCAGCAC GTACGCGGGG ATCCGGAAAG TCCCGTCCTT TACCTTCACA
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATCA GTGAACCTGC CACACAGCCA TGGCTGGCGG ACACGTGGCC CAACACAGGC
    AACAGTCACA ATGACTGCTC CATCAACTGC TGCACAGCCA GCAACGGCAA CAGCGACAGC
    AACCTGACCA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ACGCCACCAC GCAGCTCATC
    CAGGCAAATC TCAGCAACAA TATGAATAAC GGCGGCGGGG ACTCCAGCGA GAAACACTGG
    AAGCCTCCGG GGCAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACAATCC CTCCAACCAT CCCGTACAAC
    CAATCCTATG ACCAGAATAC AGGAGGATCT TACAACAGCT CAGATCGGGG CAGTAGTACA
    TCTGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCC CCAGCGCATC CTCCCCCACA CAGCAACAGC
    GAGGAATACA GCATGTCTGT AGATGAAAGC TATGATCAAG AAATGCCGTG CCCTGTTCCG
    CCTGCACGGA TGTATTTGCA ACAGGATGAA TTAGAAGAAG AGGAAGCTGA AAGAGGCCCC
    ACGCCCCCTG TCCGGGGGGC AGCTTCGTCC CCAGCTGCTG TGTCCTACAG CCATCAGTCC
    ACGGCCACTC TCACCCCGTC TCCCCAGGAA GAGCTCCAGC CTATGTTACA GGATTGTCCG
    GAGGACCTCG GCCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCGGT GAGTCCTCCT
    CCACCACCAC GGCCAATTTC CCCACCACAT ACTTACGGCT ACATTTCAGG ACCCCTGGTC
    TCAGACATGG ATACGGATGC CCCAGAAGAG GAGGAAGACG AAGCTGACAT GGAAGTTGCT
    AAGATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTTGAAC AGACCCCAGC ATCTAGTGTT
    GGGGACCTCG AGAGCTCGGT CACTGGCTCC ATGATCAATG GCTGGGGCTC AGCCTCAGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CGGATGGTTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCCGCA GCCGCCGAGT ATGCGGGCCT GAAGGTGGCT
    CGCCGCCAGA TGCAGGATGC TGCTGGCCGC CGACACTTCC ATGCCTCCCA GTGCCCTAGG
    CCCACGAGTC CTGTGTCTAC AGACAGCAAC ATGAGTGCTG CTGTAATCCA GAAAGCCAGA
    CCCACCAAGA AACAGAAGCA CCAGCCAGGA CATCTGCGCA GAGAGGCCTA CACAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCAGTGTCCA GTCCAAGGCA
    CAGCTGGAGG CACGGCCTAT AATGGGGCCA AAGCTCGCTT CTATAGAAGC GAGAGCAGAC
    AGATCATCAG ACAGAAAAGG CGGCAGTTAC AAGGGGAGAG AAGCTCTGGA CGGAAGACAA
    GTCACTGACC TGCGAACAAG CCCAGGTGAC CCCAGAGAAG CACAGGAGCA GCCAAACGAA
    GGGAAAGCAC GAGGGACGAA GACAGCAAAG CGAGACCTTC CACCAGCGAA GACACACCTC
    ATCCCAGAGG ATATCCTTCC GTACTGTAGG CCTACTTTCC CAACGTCAAA CAACCCTAGG
    GATCCCAGTT CTTCAAGCTC AATGTCATCA AGAGGCTCAG GAAGCCGACA GAGAGAGCAA
    GCAAATGTGG GCCGAAGAAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57715
    Clone ID Related Accession (Same CDS sequence) XM_017598063.1
    Accession Version XM_017598063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT CCCACCGCGC ATTGTTGAGC ACCCTTCAGA CCTGATCGTC
    TCTAAAGGGG AACCCGCCAC CCTCAACTGT AAAGCTGAAG GCCGCCCTAC CCCGACTATA
    GAATGGTACA AGGGCGGCGA AAGAGTGGAA ACGGACAAAG ACGACCCTCG CTCACACCGA
    ATGCTGCTGC CAAGTGGATC TTTATTTTTC TTACGCATAG TGCATGGACG GAAGAGTAGG
    CCTGACGAAG GGGTCTACAT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AATGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTT
    ATGGTGGCGG TGGGCGAGCC TGCAGTAATG GAATGCCAGC CTCCTCGGGG CCACCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGACGA GCGAATCACT
    ATCCGAGGCG GGAAGCTCAT GATCACGTAC ACCCGTAAAA GCGATGCTGG CAAGTATGTT
    TGTGTTGGTA CCAACATGGT AGGGGAGCGG GAGAGCGAAG TCGCGGAGCT GACTGTCTTA
    GAGAGACCGT CCTTTGTAAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCT
    GAGTTTAAGT GTGAGGCCCG AGGAGACCCC GTGCCTACGG TTCGGTGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAGATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACAGCTG GCGACATGGG ATCATACACT TGTGTTGCAG AAAATATGGT TGGGAAAGCC
    GAAGCATCAG CCACTTTGAC AGTTCAAGAG CCACCACATT TTGTCGTGAA GCCTCGAGAC
    CAGGTGGTTG CCTTGGGGCG AACTGTGACT TTTCAGTGTG AAGCAACTGG AAATCCTCAA
    CCAGCTATCT TTTGGCGGAG AGAGGGGAGT CAGAATCTGC TTTTCTCATA CCAGCCTCCA
    CAGTCCTCTA GCCGGTTTTC AGTTTCCCAG ACGGGGGACC TCACTGTTAC GAACGTCCAG
    AGGTCTGATG TCGGTTACTA TATCTGTCAG ACTTTGAACG TGGCTGGAAG CATCATCACA
    AAGGCATATT TGGAAGTTAC TGATGTGATT GCGGACCGGC CTCCCCCAGT CATCCGACAA
    GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGTACTTTAA CTCTCAGCTG TGTGGCCACA
    GGCAGTCCCG TGCCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT
    TCTCGGATCA AACAGCTGGA GAGCGGGGTC CTGCAGATCC GCTACGCTAA GCTGGGTGAC
    ACCGGTCGGT ACACCTGCAC TGCTTCGACC CCCAGTGGTG AGGCCACGTG GAGCGCTTAC
    ATCGAAGTCC AGGAATTTGG AGTTCCAGTT CAACCTCCAA GACCCACCGA CCCCAATTTA
    ATCCCCAGTG CGCCTTCAAA GCCTGAAGTC ACAGATGTGA GCAAAAACAC AGTCACTTTA
    TTGTGGCAGC CTAACCTGAA CTCAGGAGCA ACCCCAACGT CTTATATTAT AGAAGCCTTC
    AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTGGCAGAAA ATGTGAAAAC AGAAACATTC
    GCCATCAAAG GGCTCAAACC TAATGCGATC TACCTTTTCC TGGTGAGGGC AGCAAATGCC
    TATGGGATTA GCGATCCAAG CCAAATATCC GATCCAGTGA AAACACAAGA TGTCCCACCA
    ACCACCCAAG GGGTGGACCA CAAGCAGGTC CAGAGAGAAC TGGGCAATGT TGTCTTGCAT
    CTCCACAACC CTACCATCCT TTCTTCCTCT TCTGTCGAAG TGCACTGGAC GGTGGACCAA
    CAATCTCAAT ATATTCAAGG TTATAAAATT CTCTACCGAC CATCTGGAGC CAGCCATGGC
    GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACCCCAACCA AAAATAGTGT GGTGATCCCC
    GATCTGAGAA AAGGCGTCAA CTACGAGATT AAGGCCCGCC CATTTTTTAA TGAGTTCCAA
    GGAGCAGATA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC
    CGAAGTGTCA CTGTATCCAA GAATGATGGA AATGGGACAG CAATTCTGGT CACTTGGCAA
    CCGCCTCCTG AAGACACACA GAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGG
    AATGAGACTA GGTATCACAT AAACAAGACA GTAGATGGCT CTACCTTTTC GGTGGTCATC
    CCCTCTCTGG TTCCTGGGAT CCGCTACAGT GTAGAAGTGG CTGCAAGCAC TGGGGCTGGC
    CCTGGGGTGA AGAGTGAACC CCAGTTCATC CAGTTAGATT CCCATGGAAA CCCCGTGTCT
    CCCGAGGACC AAGTGAGCCT GGCTCAGCAG ATCTCCGACG TGGTGAAGCA GCCAGCCTTC
    ATTGCGGGCA TCGGGGCCGC CTGTTGGATC ATTCTCATGG TCTTCAGTAT CTGGCTTTAC
    CGACACCGCA AGAAGAGAAA CGGGCTCAGC AGCACGTACG CGGGGATCCG GAAAGTCCCG
    TCCTTTACCT TCACACCAAC AGTAACTTAT CAAAGAGGAG GCGAAGCCGT CAGCAGTGGA
    GGGAGGCCGG GACTCCTCAA CATCAGTGAA CCTGCCACAC AGCCATGGCT GGCGGACACG
    TGGCCCAACA CAGGCAACAG TCACAATGAC TGCTCCATCA ACTGCTGCAC AGCCAGCAAC
    GGCAACAGCG ACAGCAACCT GACCACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT
    AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT
    GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG
    GACGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACTCCCT ACGCCACCAC GCAGCTCATC
    CAGGCAAATC TCAGCAACAA TATGAATAAC GGCGGCGGGG ACTCCAGCGA GAAACACTGG
    AAGCCTCCGG GGCAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACAATCC CTCCAACCAT CCCGTACAAC
    CAATCCTATG ACCAGAATAC AGGAGGATCT TACAACAGCT CAGATCGGGG CAGTAGTACA
    TCTGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCC CCAGCGCATC CTCCCCCACA CAGCAACAGC
    GAGGAATACA GCATGTCTGT AGATGAAAGC TATGATCAAG AAATGCCGTG CCCTGTTCCG
    CCTGCACGGA TGTATTTGCA ACAGGATGAA TTAGAAGAAG AGGAAGCTGA AAGAGGCCCC
    ACGCCCCCTG TCCGGGGGGC AGCTTCGTCC CCAGCTGCTG TGTCCTACAG CCATCAGTCC
    ACGGCCACTC TCACCCCGTC TCCCCAGGAA GAGCTCCAGC CTATGTTACA GGATTGTCCG
    GAGGACCTCG GCCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCGGT GAGTCCTCCT
    CCACCACCAC GGCCAATTTC CCCACCACAT ACTTACGGCT ACATTTCAGG ACCCCTGGTC
    TCAGACATGG ATACGGATGC CCCAGAAGAG GAGGAAGACG AAGCTGACAT GGAAGTTGCT
    AAGATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTTGAAC AGACCCCAGC ATCTAGTGTT
    GGGGACCTCG AGAGCTCGGT CACTGGCTCC ATGATCAATG GCTGGGGCTC AGCCTCAGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CGGATGGTTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCCGCA GCCGCCGAGT ATGCGGGCCT GAAGGTGGCT
    CGCCGCCAGA TGCAGGATGC TGCTGGCCGC CGACACTTCC ATGCCTCCCA GTGCCCTAGG
    CCCACGAGTC CTGTGTCTAC AGACAGCAAC ATGAGTGCTG CTGTAATCCA GAAAGCCAGA
    CCCACCAAGA AACAGAAGCA CCAGCCAGGA CATCTGCGCA GAGAGGCCTA CACAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCAGTGTCCA GTCCAAGGCA
    CAGCTGGAGG CACGGCCTAT AATGGGGCCA AAGCTCGCTT CTATAGAAGC GAGAGCAGAC
    AGATCATCAG ACAGAAAAGG CGGCAGTTAC AAGGGGAGAG AAGCTCTGGA CGGAAGACAA
    GTCACTGACC TGCGAACAAG CCCAGGTGAC CCCAGAGAAG CACAGGAGCA GCCAAACGAA
    GGGAAAGCAC GAGGGACGAA GACAGCAAAG CGAGACCTTC CACCAGCGAA GACACACCTC
    ATCCCAGAGG ATATCCTTCC GTACTGTAGG CCTACTTTCC CAACGTCAAA CAACCCTAGG
    GATCCCAGTT CTTCAAGCTC AATGTCATCA AGAGGCTCAG GAAGCCGACA GAGAGAGCAA
    GCAAATGTGG GCCGAAGAAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453552.1
    CDS960..5798
    Translation

    Target ORF information:

    RefSeq Version XM_017598063.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT CCCACCGCGC ATTGTTGAGC ACCCTTCAGA CCTGATCGTC
    TCTAAAGGGG AACCCGCCAC CCTCAACTGT AAAGCTGAAG GCCGCCCTAC CCCGACTATA
    GAATGGTACA AGGGCGGCGA AAGAGTGGAA ACGGACAAAG ACGACCCTCG CTCACACCGA
    ATGCTGCTGC CAAGTGGATC TTTATTTTTC TTACGCATAG TGCATGGACG GAAGAGTAGG
    CCTGACGAAG GGGTCTACAT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AATGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTT
    ATGGTGGCGG TGGGCGAGCC TGCAGTAATG GAATGCCAGC CTCCTCGGGG CCACCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGACGA GCGAATCACT
    ATCCGAGGCG GGAAGCTCAT GATCACGTAC ACCCGTAAAA GCGATGCTGG CAAGTATGTT
    TGTGTTGGTA CCAACATGGT AGGGGAGCGG GAGAGCGAAG TCGCGGAGCT GACTGTCTTA
    GAGAGACCGT CCTTTGTAAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCT
    GAGTTTAAGT GTGAGGCCCG AGGAGACCCC GTGCCTACGG TTCGGTGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAGATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACAGCTG GCGACATGGG ATCATACACT TGTGTTGCAG AAAATATGGT TGGGAAAGCC
    GAAGCATCAG CCACTTTGAC AGTTCAAGAG CCACCACATT TTGTCGTGAA GCCTCGAGAC
    CAGGTGGTTG CCTTGGGGCG AACTGTGACT TTTCAGTGTG AAGCAACTGG AAATCCTCAA
    CCAGCTATCT TTTGGCGGAG AGAGGGGAGT CAGAATCTGC TTTTCTCATA CCAGCCTCCA
    CAGTCCTCTA GCCGGTTTTC AGTTTCCCAG ACGGGGGACC TCACTGTTAC GAACGTCCAG
    AGGTCTGATG TCGGTTACTA TATCTGTCAG ACTTTGAACG TGGCTGGAAG CATCATCACA
    AAGGCATATT TGGAAGTTAC TGATGTGATT GCGGACCGGC CTCCCCCAGT CATCCGACAA
    GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGTACTTTAA CTCTCAGCTG TGTGGCCACA
    GGCAGTCCCG TGCCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT
    TCTCGGATCA AACAGCTGGA GAGCGGGGTC CTGCAGATCC GCTACGCTAA GCTGGGTGAC
    ACCGGTCGGT ACACCTGCAC TGCTTCGACC CCCAGTGGTG AGGCCACGTG GAGCGCTTAC
    ATCGAAGTCC AGGAATTTGG AGTTCCAGTT CAACCTCCAA GACCCACCGA CCCCAATTTA
    ATCCCCAGTG CGCCTTCAAA GCCTGAAGTC ACAGATGTGA GCAAAAACAC AGTCACTTTA
    TTGTGGCAGC CTAACCTGAA CTCAGGAGCA ACCCCAACGT CTTATATTAT AGAAGCCTTC
    AGCCATGCAT CTGGTAGCAG CTGGCAGACC GTGGCAGAAA ATGTGAAAAC AGAAACATTC
    GCCATCAAAG GGCTCAAACC TAATGCGATC TACCTTTTCC TGGTGAGGGC AGCAAATGCC
    TATGGGATTA GCGATCCAAG CCAAATATCC GATCCAGTGA AAACACAAGA TGTCCCACCA
    ACCACCCAAG GGGTGGACCA CAAGCAGGTC CAGAGAGAAC TGGGCAATGT TGTCTTGCAT
    CTCCACAACC CTACCATCCT TTCTTCCTCT TCTGTCGAAG TGCACTGGAC GGTGGACCAA
    CAATCTCAAT ATATTCAAGG TTATAAAATT CTCTACCGAC CATCTGGAGC CAGCCATGGC
    GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACCCCAACCA AAAATAGTGT GGTGATCCCC
    GATCTGAGAA AAGGCGTCAA CTACGAGATT AAGGCCCGCC CATTTTTTAA TGAGTTCCAA
    GGAGCAGATA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC
    CGAAGTGTCA CTGTATCCAA GAATGATGGA AATGGGACAG CAATTCTGGT CACTTGGCAA
    CCGCCTCCTG AAGACACACA GAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGG
    AATGAGACTA GGTATCACAT AAACAAGACA GTAGATGGCT CTACCTTTTC GGTGGTCATC
    CCCTCTCTGG TTCCTGGGAT CCGCTACAGT GTAGAAGTGG CTGCAAGCAC TGGGGCTGGC
    CCTGGGGTGA AGAGTGAACC CCAGTTCATC CAGTTAGATT CCCATGGAAA CCCCGTGTCT
    CCCGAGGACC AAGTGAGCCT GGCTCAGCAG ATCTCCGACG TGGTGAAGCA GCCAGCCTTC
    ATTGCGGGCA TCGGGGCCGC CTGTTGGATC ATTCTCATGG TCTTCAGTAT CTGGCTTTAC
    CGACACCGCA AGAAGAGAAA CGGGCTCAGC AGCACGTACG CGGGGATCCG GAAAGTCCCG
    TCCTTTACCT TCACACCAAC AGTAACTTAT CAAAGAGGAG GCGAAGCCGT CAGCAGTGGA
    GGGAGGCCGG GACTCCTCAA CATCAGTGAA CCTGCCACAC AGCCATGGCT GGCGGACACG
    TGGCCCAACA CAGGCAACAG TCACAATGAC TGCTCCATCA ACTGCTGCAC AGCCAGCAAC
    GGCAACAGCG ACAGCAACCT GACCACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT
    AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT
    GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG
    GACGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACTCCCT ACGCCACCAC GCAGCTCATC
    CAGGCAAATC TCAGCAACAA TATGAATAAC GGCGGCGGGG ACTCCAGCGA GAAACACTGG
    AAGCCTCCGG GGCAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACAATCC CTCCAACCAT CCCGTACAAC
    CAATCCTATG ACCAGAATAC AGGAGGATCT TACAACAGCT CAGATCGGGG CAGTAGTACA
    TCTGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCC CCAGCGCATC CTCCCCCACA CAGCAACAGC
    GAGGAATACA GCATGTCTGT AGATGAAAGC TATGATCAAG AAATGCCGTG CCCTGTTCCG
    CCTGCACGGA TGTATTTGCA ACAGGATGAA TTAGAAGAAG AGGAAGCTGA AAGAGGCCCC
    ACGCCCCCTG TCCGGGGGGC AGCTTCGTCC CCAGCTGCTG TGTCCTACAG CCATCAGTCC
    ACGGCCACTC TCACCCCGTC TCCCCAGGAA GAGCTCCAGC CTATGTTACA GGATTGTCCG
    GAGGACCTCG GCCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCGGT GAGTCCTCCT
    CCACCACCAC GGCCAATTTC CCCACCACAT ACTTACGGCT ACATTTCAGG ACCCCTGGTC
    TCAGACATGG ATACGGATGC CCCAGAAGAG GAGGAAGACG AAGCTGACAT GGAAGTTGCT
    AAGATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTTGAAC AGACCCCAGC ATCTAGTGTT
    GGGGACCTCG AGAGCTCGGT CACTGGCTCC ATGATCAATG GCTGGGGCTC AGCCTCAGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CGGATGGTTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCCGCA GCCGCCGAGT ATGCGGGCCT GAAGGTGGCT
    CGCCGCCAGA TGCAGGATGC TGCTGGCCGC CGACACTTCC ATGCCTCCCA GTGCCCTAGG
    CCCACGAGTC CTGTGTCTAC AGACAGCAAC ATGAGTGCTG CTGTAATCCA GAAAGCCAGA
    CCCACCAAGA AACAGAAGCA CCAGCCAGGA CATCTGCGCA GAGAGGCCTA CACAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCAGTGTCCA GTCCAAGGCA
    CAGCTGGAGG CACGGCCTAT AATGGGGCCA AAGCTCGCTT CTATAGAAGC GAGAGCAGAC
    AGATCATCAG ACAGAAAAGG CGGCAGTTAC AAGGGGAGAG AAGCTCTGGA CGGAAGACAA
    GTCACTGACC TGCGAACAAG CCCAGGTGAC CCCAGAGAAG CACAGGAGCA GCCAAACGAA
    GGGAAAGCAC GAGGGACGAA GACAGCAAAG CGAGACCTTC CACCAGCGAA GACACACCTC
    ATCCCAGAGG ATATCCTTCC GTACTGTAGG CCTACTTTCC CAACGTCAAA CAACCCTAGG
    GATCCCAGTT CTTCAAGCTC AATGTCATCA AGAGGCTCAG GAAGCCGACA GAGAGAGCAA
    GCAAATGTGG GCCGAAGAAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa57716
    Clone ID Related Accession (Same CDS sequence) XM_017598064.1
    Accession Version XM_017598064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5028bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGAGCCAC CACATTTTGT CGTGAAGCCT CGAGACCAGG TGGTTGCCTT GGGGCGAACT
    GTGACTTTTC AGTGTGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GCGGAGAGAG
    GGGAGTCAGA ATCTGCTTTT CTCATACCAG CCTCCACAGT CCTCTAGCCG GTTTTCAGTT
    TCCCAGACGG GGGACCTCAC TGTTACGAAC GTCCAGAGGT CTGATGTCGG TTACTATATC
    TGTCAGACTT TGAACGTGGC TGGAAGCATC ATCACAAAGG CATATTTGGA AGTTACTGAT
    GTGATTGCGG ACCGGCCTCC CCCAGTCATC CGACAAGGTC CTGTGAATCA GACTGTAGCA
    GTGGATGGTA CTTTAACTCT CAGCTGTGTG GCCACAGGCA GTCCCGTGCC CACAATTCTG
    TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGC
    GGGGTCCTGC AGATCCGCTA CGCTAAGCTG GGTGACACCG GTCGGTACAC CTGCACTGCT
    TCGACCCCCA GTGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGAGTT
    CCAGTTCAAC CTCCAAGACC CACCGACCCC AATTTAATCC CCAGTGCGCC TTCAAAGCCT
    GAAGTCACAG ATGTGAGCAA AAACACAGTC ACTTTATTGT GGCAGCCTAA CCTGAACTCA
    GGAGCAACCC CAACGTCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG
    CAGACCGTGG CAGAAAATGT GAAAACAGAA ACATTCGCCA TCAAAGGGCT CAAACCTAAT
    GCGATCTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GGATTAGCGA TCCAAGCCAA
    ATATCCGATC CAGTGAAAAC ACAAGATGTC CCACCAACCA CCCAAGGGGT GGACCACAAG
    CAGGTCCAGA GAGAACTGGG CAATGTTGTC TTGCATCTCC ACAACCCTAC CATCCTTTCT
    TCCTCTTCTG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGTTAT
    AAAATTCTCT ACCGACCATC TGGAGCCAGC CATGGCGAAT CGGAGTGGTT AGTTTTTGAA
    GTGAGGACCC CAACCAAAAA TAGTGTGGTG ATCCCCGATC TGAGAAAAGG CGTCAACTAC
    GAGATTAAGG CCCGCCCATT TTTTAATGAG TTCCAAGGAG CAGATAGTGA AATCAAATTT
    GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTCACTGT ATCCAAGAAT
    GATGGAAATG GGACAGCAAT TCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAGAAT
    GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGGAATG AGACTAGGTA TCACATAAAC
    AAGACAGTAG ATGGCTCTAC CTTTTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGC
    TACAGTGTAG AAGTGGCTGC AAGCACTGGG GCTGGCCCTG GGGTGAAGAG TGAACCCCAG
    TTCATCCAGT TAGATTCCCA TGGAAACCCC GTGTCTCCCG AGGACCAAGT GAGCCTGGCT
    CAGCAGATCT CCGACGTGGT GAAGCAGCCA GCCTTCATTG CGGGCATCGG GGCCGCCTGT
    TGGATCATTC TCATGGTCTT CAGTATCTGG CTTTACCGAC ACCGCAAGAA GAGAAACGGG
    CTCAGCAGCA CGTACGCGGG GATCCGGAAA GTAACTTATC AAAGAGGAGG CGAAGCCGTC
    AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTG
    GCGGACACGT GGCCCAACAC AGGCAACAGT CACAATGACT GCTCCATCAA CTGCTGCACA
    GCCAGCAACG GCAACAGCGA CAGCAACCTG ACCACCTACA GTCGCCCAGC TGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA CGCCACCACG
    CAGCTCATCC AGGCAAATCT CAGCAACAAT ATGAATAACG GCGGCGGGGA CTCCAGCGAG
    AAACACTGGA AGCCTCCGGG GCAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACAATCCC TCCAACCATC
    CCGTACAACC AATCCTATGA CCAGAATACA GGAGGATCTT ACAACAGCTC AGATCGGGGC
    AGTAGTACAT CTGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG
    CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCCC CAGCGCATCC TCCCCCACAC
    AGCAACAGCG AGGAATACAG CATGTCTGTA GATGAAAGCT ATGATCAAGA AATGCCGTGC
    CCTGTTCCGC CTGCACGGAT GTATTTGCAA CAGGATGAAT TAGAAGAAGA GGAAGCTGAA
    AGAGGCCCCA CGCCCCCTGT CCGGGGGGCA GCTTCGTCCC CAGCTGCTGT GTCCTACAGC
    CATCAGTCCA CGGCCACTCT CACCCCGTCT CCCCAGGAAG AGCTCCAGCC TATGTTACAG
    GATTGTCCGG AGGACCTCGG CCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCGGTG
    AGTCCTCCTC CACCACCACG GCCAATTTCC CCACCACATA CTTACGGCTA CATTTCAGGA
    CCCCTGGTCT CAGACATGGA TACGGATGCC CCAGAAGAGG AGGAAGACGA AGCTGACATG
    GAAGTTGCTA AGATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTTGAACA GACCCCAGCA
    TCTAGTGTTG GGGACCTCGA GAGCTCGGTC ACTGGCTCCA TGATCAATGG CTGGGGCTCA
    GCCTCAGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC GGATGGTTCC
    TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCCGCAG CCGCCGAGTA TGCGGGCCTG
    AAGGTGGCTC GCCGCCAGAT GCAGGATGCT GCTGGCCGCC GACACTTCCA TGCCTCCCAG
    TGCCCTAGGC CCACGAGTCC TGTGTCTACA GACAGCAACA TGAGTGCTGC TGTAATCCAG
    AAAGCCAGAC CCACCAAGAA ACAGAAGCAC CAGCCAGGAC ATCTGCGCAG AGAGGCCTAC
    ACAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CAGTGTCCAG
    TCCAAGGCAC AGCTGGAGGC ACGGCCTATA ATGGGGCCAA AGCTCGCTTC TATAGAAGCG
    AGAGCAGACA GATCATCAGA CAGAAAAGGC GGCAGTTACA AGGGGAGAGA AGCTCTGGAC
    GGAAGACAAG TCACTGACCT GCGAACAAGC CCAGGTGACC CCAGAGAAGC ACAGGAGCAG
    CCAAACGAAG GGAAAGCACG AGGGACGAAG ACAGCAAAGC GAGACCTTCC ACCAGCGAAG
    ACACACCTCA TCCCAGAGGA TATCCTTCCG TACTGTAGGC CTACTTTCCC AACGTCAAAC
    AACCCTAGGG ATCCCAGTTC TTCAAGCTCA ATGTCATCAA GAGGCTCAGG AAGCCGACAG
    AGAGAGCAAG CAAATGTGGG CCGAAGAAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT
    GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017453553.1
    CDS241..5268
    Translation

    Target ORF information:

    RefSeq Version XM_017598064.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTGCTGA GAGCTGCACA GGGCTCAGTG TACTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCA
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GGACGCCAGC CCCCACCTCT
    GATAATGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TCCGTCAGGA AGATTTCCCA
    CCGCGCATTG TTGAGCACCC TTCAGACCTG ATCGTCTCTA AAGGGGAACC CGCCACCCTC
    AACTGTAAAG CTGAAGGCCG CCCTACCCCG ACTATAGAAT GGTACAAGGG CGGCGAAAGA
    GTGGAAACGG ACAAAGACGA CCCTCGCTCA CACCGAATGC TGCTGCCAAG TGGATCTTTA
    TTTTTCTTAC GCATAGTGCA TGGACGGAAG AGTAGGCCTG ACGAAGGGGT CTACATCTGT
    GTAGCAAGGA ATTACCTCGG AGAGGCTGTG AGCCACAATG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTTATGG TGGCGGTGGG CGAGCCTGCA
    GTAATGGAAT GCCAGCCTCC TCGGGGCCAC CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGACGAGCGA ATCACTATCC GAGGCGGGAA GCTCATGATC
    ACGTACACCC GTAAAAGCGA TGCTGGCAAG TATGTTTGTG TTGGTACCAA CATGGTAGGG
    GAGCGGGAGA GCGAAGTCGC GGAGCTGACT GTCTTAGAGA GACCGTCCTT TGTAAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCTGAGT TTAAGTGTGA GGCCCGAGGA
    GACCCCGTGC CTACGGTTCG GTGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAGATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CAGCTGGCGA CATGGGATCA
    TACACTTGTG TTGCAGAAAA TATGGTTGGG AAAGCCGAAG CATCAGCCAC TTTGACAGTT
    CAAGAGCCAC CACATTTTGT CGTGAAGCCT CGAGACCAGG TGGTTGCCTT GGGGCGAACT
    GTGACTTTTC AGTGTGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GCGGAGAGAG
    GGGAGTCAGA ATCTGCTTTT CTCATACCAG CCTCCACAGT CCTCTAGCCG GTTTTCAGTT
    TCCCAGACGG GGGACCTCAC TGTTACGAAC GTCCAGAGGT CTGATGTCGG TTACTATATC
    TGTCAGACTT TGAACGTGGC TGGAAGCATC ATCACAAAGG CATATTTGGA AGTTACTGAT
    GTGATTGCGG ACCGGCCTCC CCCAGTCATC CGACAAGGTC CTGTGAATCA GACTGTAGCA
    GTGGATGGTA CTTTAACTCT CAGCTGTGTG GCCACAGGCA GTCCCGTGCC CACAATTCTG
    TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGC
    GGGGTCCTGC AGATCCGCTA CGCTAAGCTG GGTGACACCG GTCGGTACAC CTGCACTGCT
    TCGACCCCCA GTGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGAGTT
    CCAGTTCAAC CTCCAAGACC CACCGACCCC AATTTAATCC CCAGTGCGCC TTCAAAGCCT
    GAAGTCACAG ATGTGAGCAA AAACACAGTC ACTTTATTGT GGCAGCCTAA CCTGAACTCA
    GGAGCAACCC CAACGTCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG
    CAGACCGTGG CAGAAAATGT GAAAACAGAA ACATTCGCCA TCAAAGGGCT CAAACCTAAT
    GCGATCTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GGATTAGCGA TCCAAGCCAA
    ATATCCGATC CAGTGAAAAC ACAAGATGTC CCACCAACCA CCCAAGGGGT GGACCACAAG
    CAGGTCCAGA GAGAACTGGG CAATGTTGTC TTGCATCTCC ACAACCCTAC CATCCTTTCT
    TCCTCTTCTG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGTTAT
    AAAATTCTCT ACCGACCATC TGGAGCCAGC CATGGCGAAT CGGAGTGGTT AGTTTTTGAA
    GTGAGGACCC CAACCAAAAA TAGTGTGGTG ATCCCCGATC TGAGAAAAGG CGTCAACTAC
    GAGATTAAGG CCCGCCCATT TTTTAATGAG TTCCAAGGAG CAGATAGTGA AATCAAATTT
    GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTCACTGT ATCCAAGAAT
    GATGGAAATG GGACAGCAAT TCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAGAAT
    GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGGAATG AGACTAGGTA TCACATAAAC
    AAGACAGTAG ATGGCTCTAC CTTTTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGC
    TACAGTGTAG AAGTGGCTGC AAGCACTGGG GCTGGCCCTG GGGTGAAGAG TGAACCCCAG
    TTCATCCAGT TAGATTCCCA TGGAAACCCC GTGTCTCCCG AGGACCAAGT GAGCCTGGCT
    CAGCAGATCT CCGACGTGGT GAAGCAGCCA GCCTTCATTG CGGGCATCGG GGCCGCCTGT
    TGGATCATTC TCATGGTCTT CAGTATCTGG CTTTACCGAC ACCGCAAGAA GAGAAACGGG
    CTCAGCAGCA CGTACGCGGG GATCCGGAAA GTAACTTATC AAAGAGGAGG CGAAGCCGTC
    AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTG
    GCGGACACGT GGCCCAACAC AGGCAACAGT CACAATGACT GCTCCATCAA CTGCTGCACA
    GCCAGCAACG GCAACAGCGA CAGCAACCTG ACCACCTACA GTCGCCCAGC TGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA CGCCACCACG
    CAGCTCATCC AGGCAAATCT CAGCAACAAT ATGAATAACG GCGGCGGGGA CTCCAGCGAG
    AAACACTGGA AGCCTCCGGG GCAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACAATCCC TCCAACCATC
    CCGTACAACC AATCCTATGA CCAGAATACA GGAGGATCTT ACAACAGCTC AGATCGGGGC
    AGTAGTACAT CTGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG
    CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCCC CAGCGCATCC TCCCCCACAC
    AGCAACAGCG AGGAATACAG CATGTCTGTA GATGAAAGCT ATGATCAAGA AATGCCGTGC
    CCTGTTCCGC CTGCACGGAT GTATTTGCAA CAGGATGAAT TAGAAGAAGA GGAAGCTGAA
    AGAGGCCCCA CGCCCCCTGT CCGGGGGGCA GCTTCGTCCC CAGCTGCTGT GTCCTACAGC
    CATCAGTCCA CGGCCACTCT CACCCCGTCT CCCCAGGAAG AGCTCCAGCC TATGTTACAG
    GATTGTCCGG AGGACCTCGG CCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCGGTG
    AGTCCTCCTC CACCACCACG GCCAATTTCC CCACCACATA CTTACGGCTA CATTTCAGGA
    CCCCTGGTCT CAGACATGGA TACGGATGCC CCAGAAGAGG AGGAAGACGA AGCTGACATG
    GAAGTTGCTA AGATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTTGAACA GACCCCAGCA
    TCTAGTGTTG GGGACCTCGA GAGCTCGGTC ACTGGCTCCA TGATCAATGG CTGGGGCTCA
    GCCTCAGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC GGATGGTTCC
    TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCCGCAG CCGCCGAGTA TGCGGGCCTG
    AAGGTGGCTC GCCGCCAGAT GCAGGATGCT GCTGGCCGCC GACACTTCCA TGCCTCCCAG
    TGCCCTAGGC CCACGAGTCC TGTGTCTACA GACAGCAACA TGAGTGCTGC TGTAATCCAG
    AAAGCCAGAC CCACCAAGAA ACAGAAGCAC CAGCCAGGAC ATCTGCGCAG AGAGGCCTAC
    ACAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CAGTGTCCAG
    TCCAAGGCAC AGCTGGAGGC ACGGCCTATA ATGGGGCCAA AGCTCGCTTC TATAGAAGCG
    AGAGCAGACA GATCATCAGA CAGAAAAGGC GGCAGTTACA AGGGGAGAGA AGCTCTGGAC
    GGAAGACAAG TCACTGACCT GCGAACAAGC CCAGGTGACC CCAGAGAAGC ACAGGAGCAG
    CCAAACGAAG GGAAAGCACG AGGGACGAAG ACAGCAAAGC GAGACCTTCC ACCAGCGAAG
    ACACACCTCA TCCCAGAGGA TATCCTTCCG TACTGTAGGC CTACTTTCCC AACGTCAAAC
    AACCCTAGGG ATCCCAGTTC TTCAAGCTCA ATGTCATCAA GAGGCTCAGG AAGCCGACAG
    AGAGAGCAAG CAAATGTGGG CCGAAGAAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT
    GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43464
    Clone ID Related Accession (Same CDS sequence) XM_008768533.2
    Accession Version XM_008768533.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT CCCACCGCGC ATTGTTGAGC ACCCTTCAGA CCTGATCGTC
    TCTAAAGGGG AACCCGCCAC CCTCAACTGT AAAGCTGAAG GCCGCCCTAC CCCGACTATA
    GAATGGTACA AGGGCGGCGA AAGAGTGGAA ACGGACAAAG ACGACCCTCG CTCACACCGA
    ATGCTGCTGC CAAGTGGATC TTTATTTTTC TTACGCATAG TGCATGGACG GAAGAGTAGG
    CCTGACGAAG GGGTCTACAT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AATGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTT
    ATGGTGGCGG TGGGCGAGCC TGCAGTAATG GAATGCCAGC CTCCTCGGGG CCACCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGACGA GCGAATCACT
    ATCCGAGGCG GGAAGCTCAT GATCACGTAC ACCCGTAAAA GCGATGCTGG CAAGTATGTT
    TGTGTTGGTA CCAACATGGT AGGGGAGCGG GAGAGCGAAG TCGCGGAGCT GACTGTCTTA
    GAGAGACCGT CCTTTGTAAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCT
    GAGTTTAAGT GTGAGGCCCG AGGAGACCCC GTGCCTACGG TTCGGTGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAGATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACAGCTG GCGACATGGG ATCATACACT TGTGTTGCAG AAAATATGGT TGGGAAAGCC
    GAAGCATCAG CCACTTTGAC AGTTCAAGTT GGGTCTGAGC CACCACATTT TGTCGTGAAG
    CCTCGAGACC AGGTGGTTGC CTTGGGGCGA ACTGTGACTT TTCAGTGTGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGCGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAC
    CAGCCTCCAC AGTCCTCTAG CCGGTTTTCA GTTTCCCAGA CGGGGGACCT CACTGTTACG
    AACGTCCAGA GGTCTGATGT CGGTTACTAT ATCTGTCAGA CTTTGAACGT GGCTGGAAGC
    ATCATCACAA AGGCATATTT GGAAGTTACT GATGTGATTG CGGACCGGCC TCCCCCAGTC
    ATCCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAAC TCTCAGCTGT
    GTGGCCACAG GCAGTCCCGT GCCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGCGGGGTCC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CCGGTCGGTA CACCTGCACT GCTTCGACCC CCAGTGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGA GTTCCAGTTC AACCTCCAAG ACCCACCGAC
    CCCAATTTAA TCCCCAGTGC GCCTTCAAAG CCTGAAGTCA CAGATGTGAG CAAAAACACA
    GTCACTTTAT TGTGGCAGCC TAACCTGAAC TCAGGAGCAA CCCCAACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TGGCAGAAAA TGTGAAAACA
    GAAACATTCG CCATCAAAGG GCTCAAACCT AATGCGATCT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGGATTAG CGATCCAAGC CAAATATCCG ATCCAGTGAA AACACAAGAT
    GTCCCACCAA CCACCCAAGG GGTGGACCAC AAGCAGGTCC AGAGAGAACT GGGCAATGTT
    GTCTTGCATC TCCACAACCC TACCATCCTT TCTTCCTCTT CTGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGT TATAAAATTC TCTACCGACC ATCTGGAGCC
    AGCCATGGCG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG
    GTGATCCCCG ATCTGAGAAA AGGCGTCAAC TACGAGATTA AGGCCCGCCC ATTTTTTAAT
    GAGTTCCAAG GAGCAGATAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTCAC TGTATCCAAG AATGATGGAA ATGGGACAGC AATTCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAG AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGGA ATGAGACTAG GTATCACATA AACAAGACAG TAGATGGCTC TACCTTTTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGCTACAGTG TAGAAGTGGC TGCAAGCACT
    GGGGCTGGCC CTGGGGTGAA GAGTGAACCC CAGTTCATCC AGTTAGATTC CCATGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTGAGCCTG GCTCAGCAGA TCTCCGACGT GGTGAAGCAG
    CCAGCCTTCA TTGCGGGCAT CGGGGCCGCC TGTTGGATCA TTCTCATGGT CTTCAGTATC
    TGGCTTTACC GACACCGCAA GAAGAGAAAC GGGCTCAGCA GCACGTACGC GGGGATCCGG
    AAAGTCCCGT CCTTTACCTT CACACCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC
    AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTG
    GCGGACACGT GGCCCAACAC AGGCAACAGT CACAATGACT GCTCCATCAA CTGCTGCACA
    GCCAGCAACG GCAACAGCGA CAGCAACCTG ACCACCTACA GTCGCCCAGC TGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA CGCCACCACG
    CAGCTCATCC AGGCAAATCT CAGCAACAAT ATGAATAACG GCGGCGGGGA CTCCAGCGAG
    AAACACTGGA AGCCTCCGGG GCAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACAATCCC TCCAACCATC
    CCGTACAACC AATCCTATGA CCAGAATACA GGAGGATCTT ACAACAGCTC AGATCGGGGC
    AGTAGTACAT CTGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG
    CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCCC CAGCGCATCC TCCCCCACAC
    AGCAACAGCG AGGAATACAG CATGTCTGTA GATGAAAGCT ATGATCAAGA AATGCCGTGC
    CCTGTTCCGC CTGCACGGAT GTATTTGCAA CAGGATGAAT TAGAAGAAGA GGAAGCTGAA
    AGAGGCCCCA CGCCCCCTGT CCGGGGGGCA GCTTCGTCCC CAGCTGCTGT GTCCTACAGC
    CATCAGTCCA CGGCCACTCT CACCCCGTCT CCCCAGGAAG AGCTCCAGCC TATGTTACAG
    GATTGTCCGG AGGACCTCGG CCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCGGTG
    AGTCCTCCTC CACCACCACG GCCAATTTCC CCACCACATA CTTACGGCTA CATTTCAGGA
    CCCCTGGTCT CAGACATGGA TACGGATGCC CCAGAAGAGG AGGAAGACGA AGCTGACATG
    GAAGTTGCTA AGATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTTGAACA GACCCCAGCA
    TCTAGTGTTG GGGACCTCGA GAGCTCGGTC ACTGGCTCCA TGATCAATGG CTGGGGCTCA
    GCCTCAGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC GGATGGTTCC
    TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCCGCAG CCGCCGAGTA TGCGGGCCTG
    AAGGTGGCTC GCCGCCAGAT GCAGGATGCT GCTGGCCGCC GACACTTCCA TGCCTCCCAG
    TGCCCTAGGC CCACGAGTCC TGTGTCTACA GACAGCAACA TGAGTGCTGC TGTAATCCAG
    AAAGCCAGAC CCACCAAGAA ACAGAAGCAC CAGCCAGGAC ATCTGCGCAG AGAGGCCTAC
    ACAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CAGTGTCCAG
    TCCAAGGCAC AGCTGGAGGC ACGGCCTATA ATGGGGCCAA AGCTCGCTTC TATAGAAGCG
    AGAGCAGACA GATCATCAGA CAGAAAAGGC GGCAGTTACA AGGGGAGAGA AGCTCTGGAC
    GGAAGACAAG TCACTGACCT GCGAACAAGC CCAGGTGACC CCAGAGAAGC ACAGGAGCAG
    CCAAACGAAG GGAAAGCACG AGGGACGAAG ACAGCAAAGC GAGACCTTCC ACCAGCGAAG
    ACACACCTCA TCCCAGAGGA TATCCTTCCG TACTGTAGGC CTACTTTCCC AACGTCAAAC
    AACCCTAGGG ATCCCAGTTC TTCAAGCTCA ATGTCATCAA GAGGCTCAGG AAGCCGACAG
    AGAGAGCAAG CAAATGTGGG CCGAAGAAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT
    GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008766755.1
    CDS960..5807
    Translation

    Target ORF information:

    RefSeq Version XM_008768533.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008768533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT CCCACCGCGC ATTGTTGAGC ACCCTTCAGA CCTGATCGTC
    TCTAAAGGGG AACCCGCCAC CCTCAACTGT AAAGCTGAAG GCCGCCCTAC CCCGACTATA
    GAATGGTACA AGGGCGGCGA AAGAGTGGAA ACGGACAAAG ACGACCCTCG CTCACACCGA
    ATGCTGCTGC CAAGTGGATC TTTATTTTTC TTACGCATAG TGCATGGACG GAAGAGTAGG
    CCTGACGAAG GGGTCTACAT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AATGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTT
    ATGGTGGCGG TGGGCGAGCC TGCAGTAATG GAATGCCAGC CTCCTCGGGG CCACCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGACGA GCGAATCACT
    ATCCGAGGCG GGAAGCTCAT GATCACGTAC ACCCGTAAAA GCGATGCTGG CAAGTATGTT
    TGTGTTGGTA CCAACATGGT AGGGGAGCGG GAGAGCGAAG TCGCGGAGCT GACTGTCTTA
    GAGAGACCGT CCTTTGTAAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCT
    GAGTTTAAGT GTGAGGCCCG AGGAGACCCC GTGCCTACGG TTCGGTGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAGATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACAGCTG GCGACATGGG ATCATACACT TGTGTTGCAG AAAATATGGT TGGGAAAGCC
    GAAGCATCAG CCACTTTGAC AGTTCAAGTT GGGTCTGAGC CACCACATTT TGTCGTGAAG
    CCTCGAGACC AGGTGGTTGC CTTGGGGCGA ACTGTGACTT TTCAGTGTGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGCGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAC
    CAGCCTCCAC AGTCCTCTAG CCGGTTTTCA GTTTCCCAGA CGGGGGACCT CACTGTTACG
    AACGTCCAGA GGTCTGATGT CGGTTACTAT ATCTGTCAGA CTTTGAACGT GGCTGGAAGC
    ATCATCACAA AGGCATATTT GGAAGTTACT GATGTGATTG CGGACCGGCC TCCCCCAGTC
    ATCCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAAC TCTCAGCTGT
    GTGGCCACAG GCAGTCCCGT GCCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGCGGGGTCC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CCGGTCGGTA CACCTGCACT GCTTCGACCC CCAGTGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGA GTTCCAGTTC AACCTCCAAG ACCCACCGAC
    CCCAATTTAA TCCCCAGTGC GCCTTCAAAG CCTGAAGTCA CAGATGTGAG CAAAAACACA
    GTCACTTTAT TGTGGCAGCC TAACCTGAAC TCAGGAGCAA CCCCAACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TGGCAGAAAA TGTGAAAACA
    GAAACATTCG CCATCAAAGG GCTCAAACCT AATGCGATCT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGGATTAG CGATCCAAGC CAAATATCCG ATCCAGTGAA AACACAAGAT
    GTCCCACCAA CCACCCAAGG GGTGGACCAC AAGCAGGTCC AGAGAGAACT GGGCAATGTT
    GTCTTGCATC TCCACAACCC TACCATCCTT TCTTCCTCTT CTGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGT TATAAAATTC TCTACCGACC ATCTGGAGCC
    AGCCATGGCG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG
    GTGATCCCCG ATCTGAGAAA AGGCGTCAAC TACGAGATTA AGGCCCGCCC ATTTTTTAAT
    GAGTTCCAAG GAGCAGATAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTCAC TGTATCCAAG AATGATGGAA ATGGGACAGC AATTCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAG AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGGA ATGAGACTAG GTATCACATA AACAAGACAG TAGATGGCTC TACCTTTTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGCTACAGTG TAGAAGTGGC TGCAAGCACT
    GGGGCTGGCC CTGGGGTGAA GAGTGAACCC CAGTTCATCC AGTTAGATTC CCATGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTGAGCCTG GCTCAGCAGA TCTCCGACGT GGTGAAGCAG
    CCAGCCTTCA TTGCGGGCAT CGGGGCCGCC TGTTGGATCA TTCTCATGGT CTTCAGTATC
    TGGCTTTACC GACACCGCAA GAAGAGAAAC GGGCTCAGCA GCACGTACGC GGGGATCCGG
    AAAGTCCCGT CCTTTACCTT CACACCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC
    AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATCAGTGAAC CTGCCACACA GCCATGGCTG
    GCGGACACGT GGCCCAACAC AGGCAACAGT CACAATGACT GCTCCATCAA CTGCTGCACA
    GCCAGCAACG GCAACAGCGA CAGCAACCTG ACCACCTACA GTCGCCCAGC TGATTGTATA
    GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA CGCCACCACG
    CAGCTCATCC AGGCAAATCT CAGCAACAAT ATGAATAACG GCGGCGGGGA CTCCAGCGAG
    AAACACTGGA AGCCTCCGGG GCAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACAATCCC TCCAACCATC
    CCGTACAACC AATCCTATGA CCAGAATACA GGAGGATCTT ACAACAGCTC AGATCGGGGC
    AGTAGTACAT CTGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG
    CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCCC CAGCGCATCC TCCCCCACAC
    AGCAACAGCG AGGAATACAG CATGTCTGTA GATGAAAGCT ATGATCAAGA AATGCCGTGC
    CCTGTTCCGC CTGCACGGAT GTATTTGCAA CAGGATGAAT TAGAAGAAGA GGAAGCTGAA
    AGAGGCCCCA CGCCCCCTGT CCGGGGGGCA GCTTCGTCCC CAGCTGCTGT GTCCTACAGC
    CATCAGTCCA CGGCCACTCT CACCCCGTCT CCCCAGGAAG AGCTCCAGCC TATGTTACAG
    GATTGTCCGG AGGACCTCGG CCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCGGTG
    AGTCCTCCTC CACCACCACG GCCAATTTCC CCACCACATA CTTACGGCTA CATTTCAGGA
    CCCCTGGTCT CAGACATGGA TACGGATGCC CCAGAAGAGG AGGAAGACGA AGCTGACATG
    GAAGTTGCTA AGATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTTGAACA GACCCCAGCA
    TCTAGTGTTG GGGACCTCGA GAGCTCGGTC ACTGGCTCCA TGATCAATGG CTGGGGCTCA
    GCCTCAGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC GGATGGTTCC
    TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCCGCAG CCGCCGAGTA TGCGGGCCTG
    AAGGTGGCTC GCCGCCAGAT GCAGGATGCT GCTGGCCGCC GACACTTCCA TGCCTCCCAG
    TGCCCTAGGC CCACGAGTCC TGTGTCTACA GACAGCAACA TGAGTGCTGC TGTAATCCAG
    AAAGCCAGAC CCACCAAGAA ACAGAAGCAC CAGCCAGGAC ATCTGCGCAG AGAGGCCTAC
    ACAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CAGTGTCCAG
    TCCAAGGCAC AGCTGGAGGC ACGGCCTATA ATGGGGCCAA AGCTCGCTTC TATAGAAGCG
    AGAGCAGACA GATCATCAGA CAGAAAAGGC GGCAGTTACA AGGGGAGAGA AGCTCTGGAC
    GGAAGACAAG TCACTGACCT GCGAACAAGC CCAGGTGACC CCAGAGAAGC ACAGGAGCAG
    CCAAACGAAG GGAAAGCACG AGGGACGAAG ACAGCAAAGC GAGACCTTCC ACCAGCGAAG
    ACACACCTCA TCCCAGAGGA TATCCTTCCG TACTGTAGGC CTACTTTCCC AACGTCAAAC
    AACCCTAGGG ATCCCAGTTC TTCAAGCTCA ATGTCATCAA GAGGCTCAGG AAGCCGACAG
    AGAGAGCAAG CAAATGTGGG CCGAAGAAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT
    GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43465
    Clone ID Related Accession (Same CDS sequence) XM_008768534.2
    Accession Version XM_008768534.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT CCCACCGCGC ATTGTTGAGC ACCCTTCAGA CCTGATCGTC
    TCTAAAGGGG AACCCGCCAC CCTCAACTGT AAAGCTGAAG GCCGCCCTAC CCCGACTATA
    GAATGGTACA AGGGCGGCGA AAGAGTGGAA ACGGACAAAG ACGACCCTCG CTCACACCGA
    ATGCTGCTGC CAAGTGGATC TTTATTTTTC TTACGCATAG TGCATGGACG GAAGAGTAGG
    CCTGACGAAG GGGTCTACAT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AATGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTT
    ATGGTGGCGG TGGGCGAGCC TGCAGTAATG GAATGCCAGC CTCCTCGGGG CCACCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGACGA GCGAATCACT
    ATCCGAGGCG GGAAGCTCAT GATCACGTAC ACCCGTAAAA GCGATGCTGG CAAGTATGTT
    TGTGTTGGTA CCAACATGGT AGGGGAGCGG GAGAGCGAAG TCGCGGAGCT GACTGTCTTA
    GAGAGACCGT CCTTTGTAAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCT
    GAGTTTAAGT GTGAGGCCCG AGGAGACCCC GTGCCTACGG TTCGGTGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAGATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACAGCTG GCGACATGGG ATCATACACT TGTGTTGCAG AAAATATGGT TGGGAAAGCC
    GAAGCATCAG CCACTTTGAC AGTTCAAGTT GGGTCTGAGC CACCACATTT TGTCGTGAAG
    CCTCGAGACC AGGTGGTTGC CTTGGGGCGA ACTGTGACTT TTCAGTGTGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGCGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAC
    CAGCCTCCAC AGTCCTCTAG CCGGTTTTCA GTTTCCCAGA CGGGGGACCT CACTGTTACG
    AACGTCCAGA GGTCTGATGT CGGTTACTAT ATCTGTCAGA CTTTGAACGT GGCTGGAAGC
    ATCATCACAA AGGCATATTT GGAAGTTACT GATGTGATTG CGGACCGGCC TCCCCCAGTC
    ATCCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAAC TCTCAGCTGT
    GTGGCCACAG GCAGTCCCGT GCCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGCGGGGTCC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CCGGTCGGTA CACCTGCACT GCTTCGACCC CCAGTGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGA GTTCCAGTTC AACCTCCAAG ACCCACCGAC
    CCCAATTTAA TCCCCAGTGC GCCTTCAAAG CCTGAAGTCA CAGATGTGAG CAAAAACACA
    GTCACTTTAT TGTGGCAGCC TAACCTGAAC TCAGGAGCAA CCCCAACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TGGCAGAAAA TGTGAAAACA
    GAAACATTCG CCATCAAAGG GCTCAAACCT AATGCGATCT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGGATTAG CGATCCAAGC CAAATATCCG ATCCAGTGAA AACACAAGAT
    GTCCCACCAA CCACCCAAGG GGTGGACCAC AAGCAGGTCC AGAGAGAACT GGGCAATGTT
    GTCTTGCATC TCCACAACCC TACCATCCTT TCTTCCTCTT CTGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGT TATAAAATTC TCTACCGACC ATCTGGAGCC
    AGCCATGGCG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG
    GTGATCCCCG ATCTGAGAAA AGGCGTCAAC TACGAGATTA AGGCCCGCCC ATTTTTTAAT
    GAGTTCCAAG GAGCAGATAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTCAC TGTATCCAAG AATGATGGAA ATGGGACAGC AATTCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAG AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGGA ATGAGACTAG GTATCACATA AACAAGACAG TAGATGGCTC TACCTTTTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGCTACAGTG TAGAAGTGGC TGCAAGCACT
    GGGGCTGGCC CTGGGGTGAA GAGTGAACCC CAGTTCATCC AGTTAGATTC CCATGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTGAGCCTG GCTCAGCAGA TCTCCGACGT GGTGAAGCAG
    CCAGCCTTCA TTGCGGGCAT CGGGGCCGCC TGTTGGATCA TTCTCATGGT CTTCAGTATC
    TGGCTTTACC GACACCGCAA GAAGAGAAAC GGGCTCAGCA GCACGTACGC GGGGATCCGG
    AAAGTAACTT ATCAAAGAGG AGGCGAAGCC GTCAGCAGTG GAGGGAGGCC GGGACTCCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTGGCGGACA CGTGGCCCAA CACAGGCAAC
    AGTCACAATG ACTGCTCCAT CAACTGCTGC ACAGCCAGCA ACGGCAACAG CGACAGCAAC
    CTGACCACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGACGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACTCC CTACGCCACC ACGCAGCTCA TCCAGGCAAA TCTCAGCAAC
    AATATGAATA ACGGCGGCGG GGACTCCAGC GAGAAACACT GGAAGCCTCC GGGGCAGCAG
    AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAGCT GAACAAAGAT
    TATCGGGCAA ATGACACAAT CCCTCCAACC ATCCCGTACA ACCAATCCTA TGACCAGAAT
    ACAGGAGGAT CTTACAACAG CTCAGATCGG GGCAGTAGTA CATCTGGGAG TCAAGGCCAC
    AAGAAAGGGG CACGGACACC AAAGGCACCC AAGCAGGGTG GCATGAACTG GGCAGACTTG
    CTGCCCCCTC CCCCAGCGCA TCCTCCCCCA CACAGCAACA GCGAGGAATA CAGCATGTCT
    GTAGATGAAA GCTATGATCA AGAAATGCCG TGCCCTGTTC CGCCTGCACG GATGTATTTG
    CAACAGGATG AATTAGAAGA AGAGGAAGCT GAAAGAGGCC CCACGCCCCC TGTCCGGGGG
    GCAGCTTCGT CCCCAGCTGC TGTGTCCTAC AGCCATCAGT CCACGGCCAC TCTCACCCCG
    TCTCCCCAGG AAGAGCTCCA GCCTATGTTA CAGGATTGTC CGGAGGACCT CGGCCACATG
    CCCCACCCAC CAGACAGGAG GCGGCAGCCG GTGAGTCCTC CTCCACCACC ACGGCCAATT
    TCCCCACCAC ATACTTACGG CTACATTTCA GGACCCCTGG TCTCAGACAT GGATACGGAT
    GCCCCAGAAG AGGAGGAAGA CGAAGCTGAC ATGGAAGTTG CTAAGATGCA GACCCGAAGG
    CTGTTGTTAC GTGGGCTTGA ACAGACCCCA GCATCTAGTG TTGGGGACCT CGAGAGCTCG
    GTCACTGGCT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AAGAGGACAA CATTTCCAGT
    GGGCGCTCCA GTGTCAGTTC ATCGGATGGT TCCTTTTTCA CTGACGCTGA TTTTGCCCAG
    GCAGTTGCCG CAGCCGCCGA GTATGCGGGC CTGAAGGTGG CTCGCCGCCA GATGCAGGAT
    GCTGCTGGCC GCCGACACTT CCATGCCTCC CAGTGCCCTA GGCCCACGAG TCCTGTGTCT
    ACAGACAGCA ACATGAGTGC TGCTGTAATC CAGAAAGCCA GACCCACCAA GAAACAGAAG
    CACCAGCCAG GACATCTGCG CAGAGAGGCC TACACAGATG ATCTTCCACC CCCTCCAGTG
    CCACCACCTG CTATAAAATC GCCCAGTGTC CAGTCCAAGG CACAGCTGGA GGCACGGCCT
    ATAATGGGGC CAAAGCTCGC TTCTATAGAA GCGAGAGCAG ACAGATCATC AGACAGAAAA
    GGCGGCAGTT ACAAGGGGAG AGAAGCTCTG GACGGAAGAC AAGTCACTGA CCTGCGAACA
    AGCCCAGGTG ACCCCAGAGA AGCACAGGAG CAGCCAAACG AAGGGAAAGC ACGAGGGACG
    AAGACAGCAA AGCGAGACCT TCCACCAGCG AAGACACACC TCATCCCAGA GGATATCCTT
    CCGTACTGTA GGCCTACTTT CCCAACGTCA AACAACCCTA GGGATCCCAG TTCTTCAAGC
    TCAATGTCAT CAAGAGGCTC AGGAAGCCGA CAGAGAGAGC AAGCAAATGT GGGCCGAAGA
    AATATGGCAG AAATGCAAGT ACTTGGGGGA TTTGAAAGAG GAGATGAGAA TAATGAAGAA
    TTAGAGGAAA CTGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008766756.1
    CDS960..5780
    Translation

    Target ORF information:

    RefSeq Version XM_008768534.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008768534.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT CCCACCGCGC ATTGTTGAGC ACCCTTCAGA CCTGATCGTC
    TCTAAAGGGG AACCCGCCAC CCTCAACTGT AAAGCTGAAG GCCGCCCTAC CCCGACTATA
    GAATGGTACA AGGGCGGCGA AAGAGTGGAA ACGGACAAAG ACGACCCTCG CTCACACCGA
    ATGCTGCTGC CAAGTGGATC TTTATTTTTC TTACGCATAG TGCATGGACG GAAGAGTAGG
    CCTGACGAAG GGGTCTACAT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AATGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTT
    ATGGTGGCGG TGGGCGAGCC TGCAGTAATG GAATGCCAGC CTCCTCGGGG CCACCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGACGA GCGAATCACT
    ATCCGAGGCG GGAAGCTCAT GATCACGTAC ACCCGTAAAA GCGATGCTGG CAAGTATGTT
    TGTGTTGGTA CCAACATGGT AGGGGAGCGG GAGAGCGAAG TCGCGGAGCT GACTGTCTTA
    GAGAGACCGT CCTTTGTAAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCT
    GAGTTTAAGT GTGAGGCCCG AGGAGACCCC GTGCCTACGG TTCGGTGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAGATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACAGCTG GCGACATGGG ATCATACACT TGTGTTGCAG AAAATATGGT TGGGAAAGCC
    GAAGCATCAG CCACTTTGAC AGTTCAAGTT GGGTCTGAGC CACCACATTT TGTCGTGAAG
    CCTCGAGACC AGGTGGTTGC CTTGGGGCGA ACTGTGACTT TTCAGTGTGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGCGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAC
    CAGCCTCCAC AGTCCTCTAG CCGGTTTTCA GTTTCCCAGA CGGGGGACCT CACTGTTACG
    AACGTCCAGA GGTCTGATGT CGGTTACTAT ATCTGTCAGA CTTTGAACGT GGCTGGAAGC
    ATCATCACAA AGGCATATTT GGAAGTTACT GATGTGATTG CGGACCGGCC TCCCCCAGTC
    ATCCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAAC TCTCAGCTGT
    GTGGCCACAG GCAGTCCCGT GCCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGCGGGGTCC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CCGGTCGGTA CACCTGCACT GCTTCGACCC CCAGTGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGA GTTCCAGTTC AACCTCCAAG ACCCACCGAC
    CCCAATTTAA TCCCCAGTGC GCCTTCAAAG CCTGAAGTCA CAGATGTGAG CAAAAACACA
    GTCACTTTAT TGTGGCAGCC TAACCTGAAC TCAGGAGCAA CCCCAACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TGGCAGAAAA TGTGAAAACA
    GAAACATTCG CCATCAAAGG GCTCAAACCT AATGCGATCT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGGATTAG CGATCCAAGC CAAATATCCG ATCCAGTGAA AACACAAGAT
    GTCCCACCAA CCACCCAAGG GGTGGACCAC AAGCAGGTCC AGAGAGAACT GGGCAATGTT
    GTCTTGCATC TCCACAACCC TACCATCCTT TCTTCCTCTT CTGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGT TATAAAATTC TCTACCGACC ATCTGGAGCC
    AGCCATGGCG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG
    GTGATCCCCG ATCTGAGAAA AGGCGTCAAC TACGAGATTA AGGCCCGCCC ATTTTTTAAT
    GAGTTCCAAG GAGCAGATAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTCAC TGTATCCAAG AATGATGGAA ATGGGACAGC AATTCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAG AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGGA ATGAGACTAG GTATCACATA AACAAGACAG TAGATGGCTC TACCTTTTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGCTACAGTG TAGAAGTGGC TGCAAGCACT
    GGGGCTGGCC CTGGGGTGAA GAGTGAACCC CAGTTCATCC AGTTAGATTC CCATGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTGAGCCTG GCTCAGCAGA TCTCCGACGT GGTGAAGCAG
    CCAGCCTTCA TTGCGGGCAT CGGGGCCGCC TGTTGGATCA TTCTCATGGT CTTCAGTATC
    TGGCTTTACC GACACCGCAA GAAGAGAAAC GGGCTCAGCA GCACGTACGC GGGGATCCGG
    AAAGTAACTT ATCAAAGAGG AGGCGAAGCC GTCAGCAGTG GAGGGAGGCC GGGACTCCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTGGCGGACA CGTGGCCCAA CACAGGCAAC
    AGTCACAATG ACTGCTCCAT CAACTGCTGC ACAGCCAGCA ACGGCAACAG CGACAGCAAC
    CTGACCACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC
    AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGACGGGCG TTTTGTCAAT
    CCATCAGGGC AGCCCACTCC CTACGCCACC ACGCAGCTCA TCCAGGCAAA TCTCAGCAAC
    AATATGAATA ACGGCGGCGG GGACTCCAGC GAGAAACACT GGAAGCCTCC GGGGCAGCAG
    AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAGCT GAACAAAGAT
    TATCGGGCAA ATGACACAAT CCCTCCAACC ATCCCGTACA ACCAATCCTA TGACCAGAAT
    ACAGGAGGAT CTTACAACAG CTCAGATCGG GGCAGTAGTA CATCTGGGAG TCAAGGCCAC
    AAGAAAGGGG CACGGACACC AAAGGCACCC AAGCAGGGTG GCATGAACTG GGCAGACTTG
    CTGCCCCCTC CCCCAGCGCA TCCTCCCCCA CACAGCAACA GCGAGGAATA CAGCATGTCT
    GTAGATGAAA GCTATGATCA AGAAATGCCG TGCCCTGTTC CGCCTGCACG GATGTATTTG
    CAACAGGATG AATTAGAAGA AGAGGAAGCT GAAAGAGGCC CCACGCCCCC TGTCCGGGGG
    GCAGCTTCGT CCCCAGCTGC TGTGTCCTAC AGCCATCAGT CCACGGCCAC TCTCACCCCG
    TCTCCCCAGG AAGAGCTCCA GCCTATGTTA CAGGATTGTC CGGAGGACCT CGGCCACATG
    CCCCACCCAC CAGACAGGAG GCGGCAGCCG GTGAGTCCTC CTCCACCACC ACGGCCAATT
    TCCCCACCAC ATACTTACGG CTACATTTCA GGACCCCTGG TCTCAGACAT GGATACGGAT
    GCCCCAGAAG AGGAGGAAGA CGAAGCTGAC ATGGAAGTTG CTAAGATGCA GACCCGAAGG
    CTGTTGTTAC GTGGGCTTGA ACAGACCCCA GCATCTAGTG TTGGGGACCT CGAGAGCTCG
    GTCACTGGCT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AAGAGGACAA CATTTCCAGT
    GGGCGCTCCA GTGTCAGTTC ATCGGATGGT TCCTTTTTCA CTGACGCTGA TTTTGCCCAG
    GCAGTTGCCG CAGCCGCCGA GTATGCGGGC CTGAAGGTGG CTCGCCGCCA GATGCAGGAT
    GCTGCTGGCC GCCGACACTT CCATGCCTCC CAGTGCCCTA GGCCCACGAG TCCTGTGTCT
    ACAGACAGCA ACATGAGTGC TGCTGTAATC CAGAAAGCCA GACCCACCAA GAAACAGAAG
    CACCAGCCAG GACATCTGCG CAGAGAGGCC TACACAGATG ATCTTCCACC CCCTCCAGTG
    CCACCACCTG CTATAAAATC GCCCAGTGTC CAGTCCAAGG CACAGCTGGA GGCACGGCCT
    ATAATGGGGC CAAAGCTCGC TTCTATAGAA GCGAGAGCAG ACAGATCATC AGACAGAAAA
    GGCGGCAGTT ACAAGGGGAG AGAAGCTCTG GACGGAAGAC AAGTCACTGA CCTGCGAACA
    AGCCCAGGTG ACCCCAGAGA AGCACAGGAG CAGCCAAACG AAGGGAAAGC ACGAGGGACG
    AAGACAGCAA AGCGAGACCT TCCACCAGCG AAGACACACC TCATCCCAGA GGATATCCTT
    CCGTACTGTA GGCCTACTTT CCCAACGTCA AACAACCCTA GGGATCCCAG TTCTTCAAGC
    TCAATGTCAT CAAGAGGCTC AGGAAGCCGA CAGAGAGAGC AAGCAAATGT GGGCCGAAGA
    AATATGGCAG AAATGCAAGT ACTTGGGGGA TTTGAAAGAG GAGATGAGAA TAATGAAGAA
    TTAGAGGAAA CTGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa43467
    Clone ID Related Accession (Same CDS sequence) XM_008768536.2
    Accession Version XM_008768536.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product roundabout homolog 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT CCCACCGCGC ATTGTTGAGC ACCCTTCAGA CCTGATCGTC
    TCTAAAGGGG AACCCGCCAC CCTCAACTGT AAAGCTGAAG GCCGCCCTAC CCCGACTATA
    GAATGGTACA AGGGCGGCGA AAGAGTGGAA ACGGACAAAG ACGACCCTCG CTCACACCGA
    ATGCTGCTGC CAAGTGGATC TTTATTTTTC TTACGCATAG TGCATGGACG GAAGAGTAGG
    CCTGACGAAG GGGTCTACAT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AATGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTT
    ATGGTGGCGG TGGGCGAGCC TGCAGTAATG GAATGCCAGC CTCCTCGGGG CCACCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGACGA GCGAATCACT
    ATCCGAGGCG GGAAGCTCAT GATCACGTAC ACCCGTAAAA GCGATGCTGG CAAGTATGTT
    TGTGTTGGTA CCAACATGGT AGGGGAGCGG GAGAGCGAAG TCGCGGAGCT GACTGTCTTA
    GAGAGACCGT CCTTTGTAAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCT
    GAGTTTAAGT GTGAGGCCCG AGGAGACCCC GTGCCTACGG TTCGGTGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAGATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACAGCTG GCGACATGGG ATCATACACT TGTGTTGCAG AAAATATGGT TGGGAAAGCC
    GAAGCATCAG CCACTTTGAC AGTTCAAGTT GGGTCTGAGC CACCACATTT TGTCGTGAAG
    CCTCGAGACC AGGTGGTTGC CTTGGGGCGA ACTGTGACTT TTCAGTGTGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGCGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAC
    CAGCCTCCAC AGTCCTCTAG CCGGTTTTCA GTTTCCCAGA CGGGGGACCT CACTGTTACG
    AACGTCCAGA GGTCTGATGT CGGTTACTAT ATCTGTCAGA CTTTGAACGT GGCTGGAAGC
    ATCATCACAA AGGCATATTT GGAAGTTACT GATGTGATTG CGGACCGGCC TCCCCCAGTC
    ATCCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAAC TCTCAGCTGT
    GTGGCCACAG GCAGTCCCGT GCCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGCGGGGTCC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CCGGTCGGTA CACCTGCACT GCTTCGACCC CCAGTGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGA GTTCCAGTTC AACCTCCAAG ACCCACCGAC
    CCCAATTTAA TCCCCAGTGC GCCTTCAAAG CCTGAAGTCA CAGATGTGAG CAAAAACACA
    GTCACTTTAT TGTGGCAGCC TAACCTGAAC TCAGGAGCAA CCCCAACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TGGCAGAAAA TGTGAAAACA
    GAAACATTCG CCATCAAAGG GCTCAAACCT AATGCGATCT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGGATTAG CGATCCAAGC CAAATATCCG ATCCAGTGAA AACACAAGAT
    GTCCCACCAA CCACCCAAGG GGTGGACCAC AAGCAGGTCC AGAGAGAACT GGGCAATGTT
    GTCTTGCATC TCCACAACCC TACCATCCTT TCTTCCTCTT CTGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGT TATAAAATTC TCTACCGACC ATCTGGAGCC
    AGCCATGGCG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG
    GTGATCCCCG ATCTGAGAAA AGGCGTCAAC TACGAGATTA AGGCCCGCCC ATTTTTTAAT
    GAGTTCCAAG GAGCAGATAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTCAC TGTATCCAAG AATGATGGAA ATGGGACAGC AATTCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAG AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGGA ATGAGACTAG GTATCACATA AACAAGACAG TAGATGGCTC TACCTTTTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGCTACAGTG TAGAAGTGGC TGCAAGCACT
    GGGGCTGGCC CTGGGGTGAA GAGTGAACCC CAGTTCATCC AGTTAGATTC CCATGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTGAGCCTG GCTCAGCAGA TCTCCGACGT GGTGAAGCAG
    CCAGCCTTCA TTGCGGGCAT CGGGGCCGCC TGTTGGATCA TTCTCATGGT CTTCAGTATC
    TGGCTTTACC GACACCGCAA GAAGAGAAAC GGGCTCAGCA GCACGTACGC GGGGATCCGG
    AAAGTAACTT ATCAAAGAGG AGGCGAAGCC GTCAGCAGTG GAGGGAGGCC GGGACTCCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTGGCGGACA CGTGGCCCAA CACAGGCAAC
    AGTCACAATG ACTGCTCCAT CAACTGCTGC ACAGCCAGCA ACGGCAACAG CGACAGCAAC
    CTGACCACCT ACAGTCGCCC AGGGCAGCCC ACTCCCTACG CCACCACGCA GCTCATCCAG
    GCAAATCTCA GCAACAATAT GAATAACGGC GGCGGGGACT CCAGCGAGAA ACACTGGAAG
    CCTCCGGGGC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAGCTGAACA AAGATTATCG GGCAAATGAC ACAATCCCTC CAACCATCCC GTACAACCAA
    TCCTATGACC AGAATACAGG AGGATCTTAC AACAGCTCAG ATCGGGGCAG TAGTACATCT
    GGGAGTCAAG GCCACAAGAA AGGGGCACGG ACACCAAAGG CACCCAAGCA GGGTGGCATG
    AACTGGGCAG ACTTGCTGCC CCCTCCCCCA GCGCATCCTC CCCCACACAG CAACAGCGAG
    GAATACAGCA TGTCTGTAGA TGAAAGCTAT GATCAAGAAA TGCCGTGCCC TGTTCCGCCT
    GCACGGATGT ATTTGCAACA GGATGAATTA GAAGAAGAGG AAGCTGAAAG AGGCCCCACG
    CCCCCTGTCC GGGGGGCAGC TTCGTCCCCA GCTGCTGTGT CCTACAGCCA TCAGTCCACG
    GCCACTCTCA CCCCGTCTCC CCAGGAAGAG CTCCAGCCTA TGTTACAGGA TTGTCCGGAG
    GACCTCGGCC ACATGCCCCA CCCACCAGAC AGGAGGCGGC AGCCGGTGAG TCCTCCTCCA
    CCACCACGGC CAATTTCCCC ACCACATACT TACGGCTACA TTTCAGGACC CCTGGTCTCA
    GACATGGATA CGGATGCCCC AGAAGAGGAG GAAGACGAAG CTGACATGGA AGTTGCTAAG
    ATGCAGACCC GAAGGCTGTT GTTACGTGGG CTTGAACAGA CCCCAGCATC TAGTGTTGGG
    GACCTCGAGA GCTCGGTCAC TGGCTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAAGAG
    GACAACATTT CCAGTGGGCG CTCCAGTGTC AGTTCATCGG ATGGTTCCTT TTTCACTGAC
    GCTGATTTTG CCCAGGCAGT TGCCGCAGCC GCCGAGTATG CGGGCCTGAA GGTGGCTCGC
    CGCCAGATGC AGGATGCTGC TGGCCGCCGA CACTTCCATG CCTCCCAGTG CCCTAGGCCC
    ACGAGTCCTG TGTCTACAGA CAGCAACATG AGTGCTGCTG TAATCCAGAA AGCCAGACCC
    ACCAAGAAAC AGAAGCACCA GCCAGGACAT CTGCGCAGAG AGGCCTACAC AGATGATCTT
    CCACCCCCTC CAGTGCCACC ACCTGCTATA AAATCGCCCA GTGTCCAGTC CAAGGCACAG
    CTGGAGGCAC GGCCTATAAT GGGGCCAAAG CTCGCTTCTA TAGAAGCGAG AGCAGACAGA
    TCATCAGACA GAAAAGGCGG CAGTTACAAG GGGAGAGAAG CTCTGGACGG AAGACAAGTC
    ACTGACCTGC GAACAAGCCC AGGTGACCCC AGAGAAGCAC AGGAGCAGCC AAACGAAGGG
    AAAGCACGAG GGACGAAGAC AGCAAAGCGA GACCTTCCAC CAGCGAAGAC ACACCTCATC
    CCAGAGGATA TCCTTCCGTA CTGTAGGCCT ACTTTCCCAA CGTCAAACAA CCCTAGGGAT
    CCCAGTTCTT CAAGCTCAAT GTCATCAAGA GGCTCAGGAA GCCGACAGAG AGAGCAAGCA
    AATGTGGGCC GAAGAAATAT GGCAGAAATG CAAGTACTTG GGGGATTTGA AAGAGGAGAT
    GAGAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008766758.1
    CDS960..5615
    Translation

    Target ORF information:

    RefSeq Version XM_008768536.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus roundabout guidance receptor 1 (Robo1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008768536.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTCCGTC AGGAAGATTT CCCACCGCGC ATTGTTGAGC ACCCTTCAGA CCTGATCGTC
    TCTAAAGGGG AACCCGCCAC CCTCAACTGT AAAGCTGAAG GCCGCCCTAC CCCGACTATA
    GAATGGTACA AGGGCGGCGA AAGAGTGGAA ACGGACAAAG ACGACCCTCG CTCACACCGA
    ATGCTGCTGC CAAGTGGATC TTTATTTTTC TTACGCATAG TGCATGGACG GAAGAGTAGG
    CCTGACGAAG GGGTCTACAT CTGTGTAGCA AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AATGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTT
    ATGGTGGCGG TGGGCGAGCC TGCAGTAATG GAATGCCAGC CTCCTCGGGG CCACCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGACGA GCGAATCACT
    ATCCGAGGCG GGAAGCTCAT GATCACGTAC ACCCGTAAAA GCGATGCTGG CAAGTATGTT
    TGTGTTGGTA CCAACATGGT AGGGGAGCGG GAGAGCGAAG TCGCGGAGCT GACTGTCTTA
    GAGAGACCGT CCTTTGTAAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCT
    GAGTTTAAGT GTGAGGCCCG AGGAGACCCC GTGCCTACGG TTCGGTGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAGATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACAGCTG GCGACATGGG ATCATACACT TGTGTTGCAG AAAATATGGT TGGGAAAGCC
    GAAGCATCAG CCACTTTGAC AGTTCAAGTT GGGTCTGAGC CACCACATTT TGTCGTGAAG
    CCTCGAGACC AGGTGGTTGC CTTGGGGCGA ACTGTGACTT TTCAGTGTGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGCGGAGA GAGGGGAGTC AGAATCTGCT TTTCTCATAC
    CAGCCTCCAC AGTCCTCTAG CCGGTTTTCA GTTTCCCAGA CGGGGGACCT CACTGTTACG
    AACGTCCAGA GGTCTGATGT CGGTTACTAT ATCTGTCAGA CTTTGAACGT GGCTGGAAGC
    ATCATCACAA AGGCATATTT GGAAGTTACT GATGTGATTG CGGACCGGCC TCCCCCAGTC
    ATCCGACAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GTACTTTAAC TCTCAGCTGT
    GTGGCCACAG GCAGTCCCGT GCCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGCGGGGTCC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CCGGTCGGTA CACCTGCACT GCTTCGACCC CCAGTGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGA GTTCCAGTTC AACCTCCAAG ACCCACCGAC
    CCCAATTTAA TCCCCAGTGC GCCTTCAAAG CCTGAAGTCA CAGATGTGAG CAAAAACACA
    GTCACTTTAT TGTGGCAGCC TAACCTGAAC TCAGGAGCAA CCCCAACGTC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACCG TGGCAGAAAA TGTGAAAACA
    GAAACATTCG CCATCAAAGG GCTCAAACCT AATGCGATCT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGGATTAG CGATCCAAGC CAAATATCCG ATCCAGTGAA AACACAAGAT
    GTCCCACCAA CCACCCAAGG GGTGGACCAC AAGCAGGTCC AGAGAGAACT GGGCAATGTT
    GTCTTGCATC TCCACAACCC TACCATCCTT TCTTCCTCTT CTGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGT TATAAAATTC TCTACCGACC ATCTGGAGCC
    AGCCATGGCG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG
    GTGATCCCCG ATCTGAGAAA AGGCGTCAAC TACGAGATTA AGGCCCGCCC ATTTTTTAAT
    GAGTTCCAAG GAGCAGATAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTCAC TGTATCCAAG AATGATGGAA ATGGGACAGC AATTCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAG AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGGA ATGAGACTAG GTATCACATA AACAAGACAG TAGATGGCTC TACCTTTTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGCTACAGTG TAGAAGTGGC TGCAAGCACT
    GGGGCTGGCC CTGGGGTGAA GAGTGAACCC CAGTTCATCC AGTTAGATTC CCATGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTGAGCCTG GCTCAGCAGA TCTCCGACGT GGTGAAGCAG
    CCAGCCTTCA TTGCGGGCAT CGGGGCCGCC TGTTGGATCA TTCTCATGGT CTTCAGTATC
    TGGCTTTACC GACACCGCAA GAAGAGAAAC GGGCTCAGCA GCACGTACGC GGGGATCCGG
    AAAGTAACTT ATCAAAGAGG AGGCGAAGCC GTCAGCAGTG GAGGGAGGCC GGGACTCCTC
    AACATCAGTG AACCTGCCAC ACAGCCATGG CTGGCGGACA CGTGGCCCAA CACAGGCAAC
    AGTCACAATG ACTGCTCCAT CAACTGCTGC ACAGCCAGCA ACGGCAACAG CGACAGCAAC
    CTGACCACCT ACAGTCGCCC AGGGCAGCCC ACTCCCTACG CCACCACGCA GCTCATCCAG
    GCAAATCTCA GCAACAATAT GAATAACGGC GGCGGGGACT CCAGCGAGAA ACACTGGAAG
    CCTCCGGGGC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC
    AAGCTGAACA AAGATTATCG GGCAAATGAC ACAATCCCTC CAACCATCCC GTACAACCAA
    TCCTATGACC AGAATACAGG AGGATCTTAC AACAGCTCAG ATCGGGGCAG TAGTACATCT
    GGGAGTCAAG GCCACAAGAA AGGGGCACGG ACACCAAAGG CACCCAAGCA GGGTGGCATG
    AACTGGGCAG ACTTGCTGCC CCCTCCCCCA GCGCATCCTC CCCCACACAG CAACAGCGAG
    GAATACAGCA TGTCTGTAGA TGAAAGCTAT GATCAAGAAA TGCCGTGCCC TGTTCCGCCT
    GCACGGATGT ATTTGCAACA GGATGAATTA GAAGAAGAGG AAGCTGAAAG AGGCCCCACG
    CCCCCTGTCC GGGGGGCAGC TTCGTCCCCA GCTGCTGTGT CCTACAGCCA TCAGTCCACG
    GCCACTCTCA CCCCGTCTCC CCAGGAAGAG CTCCAGCCTA TGTTACAGGA TTGTCCGGAG
    GACCTCGGCC ACATGCCCCA CCCACCAGAC AGGAGGCGGC AGCCGGTGAG TCCTCCTCCA
    CCACCACGGC CAATTTCCCC ACCACATACT TACGGCTACA TTTCAGGACC CCTGGTCTCA
    GACATGGATA CGGATGCCCC AGAAGAGGAG GAAGACGAAG CTGACATGGA AGTTGCTAAG
    ATGCAGACCC GAAGGCTGTT GTTACGTGGG CTTGAACAGA CCCCAGCATC TAGTGTTGGG
    GACCTCGAGA GCTCGGTCAC TGGCTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAAGAG
    GACAACATTT CCAGTGGGCG CTCCAGTGTC AGTTCATCGG ATGGTTCCTT TTTCACTGAC
    GCTGATTTTG CCCAGGCAGT TGCCGCAGCC GCCGAGTATG CGGGCCTGAA GGTGGCTCGC
    CGCCAGATGC AGGATGCTGC TGGCCGCCGA CACTTCCATG CCTCCCAGTG CCCTAGGCCC
    ACGAGTCCTG TGTCTACAGA CAGCAACATG AGTGCTGCTG TAATCCAGAA AGCCAGACCC
    ACCAAGAAAC AGAAGCACCA GCCAGGACAT CTGCGCAGAG AGGCCTACAC AGATGATCTT
    CCACCCCCTC CAGTGCCACC ACCTGCTATA AAATCGCCCA GTGTCCAGTC CAAGGCACAG
    CTGGAGGCAC GGCCTATAAT GGGGCCAAAG CTCGCTTCTA TAGAAGCGAG AGCAGACAGA
    TCATCAGACA GAAAAGGCGG CAGTTACAAG GGGAGAGAAG CTCTGGACGG AAGACAAGTC
    ACTGACCTGC GAACAAGCCC AGGTGACCCC AGAGAAGCAC AGGAGCAGCC AAACGAAGGG
    AAAGCACGAG GGACGAAGAC AGCAAAGCGA GACCTTCCAC CAGCGAAGAC ACACCTCATC
    CCAGAGGATA TCCTTCCGTA CTGTAGGCCT ACTTTCCCAA CGTCAAACAA CCCTAGGGAT
    CCCAGTTCTT CAAGCTCAAT GTCATCAAGA GGCTCAGGAA GCCGACAGAG AGAGCAAGCA
    AATGTGGGCC GAAGAAATAT GGCAGAAATG CAAGTACTTG GGGGATTTGA AAGAGGAGAT
    GAGAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.