Ddx25 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx25
Entrez Gene ID	58856
Full Name	DEAD-box helicase 25
Synonyms	GRTH
General protein information	Preferred Names DEAD-box helicase 25 Names ATP-dependent RNA helicase DDX25 DEAD (Asp-Glu-Ala-Asp) box helicase 25 DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 25 DEAD box protein 25 gonadotropin-regulated testicular RNA helicase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q21
Summary	protein has ATPase and RNA helicase activities; may be involved in gametogenesis and reproductive processes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031630.2	NP_113818.2	ATP-dependent RNA helicase DDX25

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DDX25	NP_001033606.1
Mus musculus (house mouse)	Ddx25	NP_038960.2
Pan troglodytes (chimpanzee)	DDX25	NP_001233379.1
	DDX25	XP_001112142.1
Canis lupus familiaris (dog)	DDX25	XP_536532.3
Xenopus tropicalis (tropical clawed frog)	ddx25	NP_001016345.1
Gallus gallus (chicken)	DDX25	XP_424667.3
Homo sapiens (human)	DDX25	NP_037396.3
Rattus norvegicus (Norway rat)	Ddx25	NP_113818.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of DDX25 in nuage components of mammalian spermatogenic cells: immunofluorescence and immunoelectron microscopic study.
Onohara Y, Yokota S
Histochemistry and cell biology137(1)37-51(2012 Jan)

Gonadotropin-regulated testicular helicase (GRTH/DDX25): a master post-transcriptional regulator of spermatogenesis.
Dufau ML, Sato H, Gutti R, Tsai-Morris CH
Advances in experimental medicine and biology70723-9(2011)

Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a transport protein involved in gene-specific mRNA export and protein translation during spermatogenesis.
Sheng Y, Tsai-Morris CH, Gutti R, Maeda Y, Dufau ML
The Journal of biological chemistry281(46)35048-56(2006 Nov)

Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is essential for spermatid development and completion of spermatogenesis.
Tsai-Morris CH, Sheng Y, Lee E, Lei KJ, Dufau ML
Proceedings of the National Academy of Sciences of the United States of America101(17)6373-8(2004 Apr)

Cell-specific and hormone-regulated expression of gonadotropin-regulated testicular RNA helicase gene (GRTH/Ddx25) resulting from alternative utilization of translation initiation codons in the rat testis.
Sheng Y, Tsai-Morris CH, Dufau ML
The Journal of biological chemistry278(30)27796-803(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that DDX25 functions in meiotic cells,intermitochondrial cement,loose aggregates of particles,chromatoid bodies,late chromatoid bodies,satellite bodies,granulated bodies,mitochondria-associated granules and reticulated bodies.
Title: Expression of DDX25 in nuage components of mammalian spermatogenic cells: immunofluorescence and immunoelectron microscopic study.

studies on GRTH [proide insights into the intrinsic requirements of spermatogenesis and open new avenues for studies on regulating spermatogenesis, infertility and male contraception
Title: Gonadotropin-regulated testicular helicase (GRTH/DDX25): a master post-transcriptional regulator of spermatogenesis.

The results of this study indicate that the mammalian GRTH/Ddx25 gene is a multifunctional RNA helicase that is an essential regulator of sperm maturation.
Title: Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a transport protein involved in gene-specific mRNA export and protein translation during spermatogenesis.

expression is regulated by gonadotropin/androgen at the translational level
Title: Cell-specific and hormone-regulated expression of gonadotropin-regulated testicular RNA helicase gene (GRTH/Ddx25) resulting from alternative utilization of translation initiation codons in the rat testis.

The following Ddx25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08313	NM_031630.2 Latest version!	Rattus norvegicus DEAD-box helicase 25 (Ddx25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx25 ( NM_031630.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113818.2 Rattus norvegicus DEAD-box helicase 25 (Ddx25), mRNA.

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CloneID	ORa08313
Clone ID Related Accession (Same CDS sequence)	NM_031630.2
Accession Version	NM_031630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent RNA helicase DDX25
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF142629.2. On Jul 24, 2003 this sequence version replaced NM_031630.1. ##Evidence-Data-START## Transcript exon combination :: AF142629.2, BC078791.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCGT TACTTTGGGG AGGCGACGCT GGGGCGGCGG AGAGCGAGCG GCTGAACAGC 
CACTTTTCAA ATCTCGTCCA CCCCAGGAAG AACCTTCGTG GTATTAGGAG TACCACAGTC 
CCAAACATAG ACGGCTCTCT TAACACGGAA GAAGATGATG ACGAAGACGA TGTAGTGGAT 
TTGGCAGCCA ATTCACTCTT AAACAAGTTA ATCCGCCAGT CCTTAGTAGA ATCCAGCCAC 
CGAGTGGAAG TCTTACAAAA GGATCCCAGC TCTCCACTCT ACTCGGTGAA AACATTTGAA 
GAGCTACGAC TAAAGGAAGA GTTACTAAAA GGGATTTATG CAATGGGATT TAACCGACCA 
TCCAAAATCC AAGAGATGGC CCTCCCCATG ATGCTCGCAC ATCCTCCCCA GAACCTCATA 
GCACAGAGCC AGTCCGGCAC AGGAAAAACC GCTGCCTTTG TCTTAGCGAT GCTAAACAGA 
GTGAATGCCT TGGAATTGTT TCCACAGTGC CTCTGCCTGG CTCCCACCTA TGAACTGGCT 
CTGCAGACCG GCCGCGTGGT TGAAAGAATG GGGAAATTCT GTGTGGATGT TGAAGTGATG 
TACGCCATTC GAGGAAACCG AATTCCTAGG GGCACCGACG TCACCAAACA GATTGTCATC 
GGCACTCCTG GGACTGTCCT AGACTGGTGC TTCAAGCGGA AACTGATTGA CTTGACAAAG 
ATCCGTGTGT TTGTCCTGGA TGAAGCAGAT GTGATGATTG ACACCCAAGG ATTCTCCGAT 
CAGAGCATCC GTATCCAAAG AGCTTTACCC TCTGAATGCC AGATGCTCCT CTTTTCAGCA 
ACCTTTGAGG ACTCTGTGTG GCAGTTTGCT GAACGAATCA TTCCTGACCC CAATGTTATC 
AAGTTGCGAA AAGAGGAACT GACCCTGAAC AACATCCGGC AGTATTACGT GTTGTGTGAG 
AACAGGAAAG ACAAATACCA AGCCCTCTGT AATATCTACG GTGGCATCAC CATTGGCCAA 
GCCATCATCT TCTGCCAGAC GCGTCGGAAT GCCAAGTGGC TGACAGTGGA GATGATGCAG 
GATGGCCACC AGGTGTCCCT GCTAAGTGGA GAACTCACGG TGGAGCAGCG GGCTTCCATC 
ATTCAGAGGT TCCGAGATGG CAAAGAGAAG GTTCTCATAA CAACCAATGT CTGTGCTCGA 
GGAATCGATG TGAAGCAGGT CACCATCGTT GTGAACTTTG ACCTCCCTGT AAACCAGTCA 
GAAGAGCCAG ACTATGAGAC CTACCTCCAC CGTATAGGGC GGACGGGACG CTTTGGGAAA 
AAAGGTCTCG CCTTCAACAT GATTGAAGTG GATAAGCTGC CCCTGCTCAT GAAAATACAA 
GACCACTTCA ATAGCAGTAT TAAGCAGCTG GACCCAGAAG ACATGGATGA GATTGAAAAG 
ATCGAATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113818.2
CDS	26..1477
Translation	MASLLWGGDAGAAESERLNSHFSNLVHPRKNLRGIRSTTVPNIDGSLNTEEDDDEDDVVDLAANSLLNKLIRQSLVESSHRVEVLQKDPSSPLYSVKTFEELRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLNRVNALELFPQCLCLAPTYELALQTGRVVERMGKFCVDVEVMYAIRGNRIPRGTDVTKQIVIGTPGTVLDWCFKRKLIDLTKIRVFVLDEADVMIDTQGFSDQSIRIQRALPSECQMLLFSATFEDSVWQFAERIIPDPNVIKLRKEELTLNNIRQYYVLCENRKDKYQALCNIYGGITIGQAIIFCQTRRNAKWLTVEMMQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLITTNVCARGIDVKQVTIVVNFDLPVNQSEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDKLPLLMKIQDHFNSSIKQLDPEDMDEIEKIEY

Target ORF information:

RefSeq Version	NM_031630.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 25 (Ddx25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031630.2

ORF Insert Sequence:

ATGGCGTCGT TACTTTGGGG AGGCGACGCT GGGGCGGCGG AGAGCGAGCG GCTGAACAGC 
CACTTTTCAA ATCTCGTCCA CCCCAGGAAG AACCTTCGTG GTATTAGGAG TACCACAGTC 
CCAAACATAG ACGGCTCTCT TAACACGGAA GAAGATGATG ACGAAGACGA TGTAGTGGAT 
TTGGCAGCCA ATTCACTCTT AAACAAGTTA ATCCGCCAGT CCTTAGTAGA ATCCAGCCAC 
CGAGTGGAAG TCTTACAAAA GGATCCCAGC TCTCCACTCT ACTCGGTGAA AACATTTGAA 
GAGCTACGAC TAAAGGAAGA GTTACTAAAA GGGATTTATG CAATGGGATT TAACCGACCA 
TCCAAAATCC AAGAGATGGC CCTCCCCATG ATGCTCGCAC ATCCTCCCCA GAACCTCATA 
GCACAGAGCC AGTCCGGCAC AGGAAAAACC GCTGCCTTTG TCTTAGCGAT GCTAAACAGA 
GTGAATGCCT TGGAATTGTT TCCACAGTGC CTCTGCCTGG CTCCCACCTA TGAACTGGCT 
CTGCAGACCG GCCGCGTGGT TGAAAGAATG GGGAAATTCT GTGTGGATGT TGAAGTGATG 
TACGCCATTC GAGGAAACCG AATTCCTAGG GGCACCGACG TCACCAAACA GATTGTCATC 
GGCACTCCTG GGACTGTCCT AGACTGGTGC TTCAAGCGGA AACTGATTGA CTTGACAAAG 
ATCCGTGTGT TTGTCCTGGA TGAAGCAGAT GTGATGATTG ACACCCAAGG ATTCTCCGAT 
CAGAGCATCC GTATCCAAAG AGCTTTACCC TCTGAATGCC AGATGCTCCT CTTTTCAGCA 
ACCTTTGAGG ACTCTGTGTG GCAGTTTGCT GAACGAATCA TTCCTGACCC CAATGTTATC 
AAGTTGCGAA AAGAGGAACT GACCCTGAAC AACATCCGGC AGTATTACGT GTTGTGTGAG 
AACAGGAAAG ACAAATACCA AGCCCTCTGT AATATCTACG GTGGCATCAC CATTGGCCAA 
GCCATCATCT TCTGCCAGAC GCGTCGGAAT GCCAAGTGGC TGACAGTGGA GATGATGCAG 
GATGGCCACC AGGTGTCCCT GCTAAGTGGA GAACTCACGG TGGAGCAGCG GGCTTCCATC 
ATTCAGAGGT TCCGAGATGG CAAAGAGAAG GTTCTCATAA CAACCAATGT CTGTGCTCGA 
GGAATCGATG TGAAGCAGGT CACCATCGTT GTGAACTTTG ACCTCCCTGT AAACCAGTCA 
GAAGAGCCAG ACTATGAGAC CTACCTCCAC CGTATAGGGC GGACGGGACG CTTTGGGAAA 
AAAGGTCTCG CCTTCAACAT GATTGAAGTG GATAAGCTGC CCCTGCTCAT GAAAATACAA 
GACCACTTCA ATAGCAGTAT TAAGCAGCTG GACCCAGAAG ACATGGATGA GATTGAAAAG 
ATCGAATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of DDX25 in nuage components of mammalian spermatogenic cells: immunofluorescence and immunoelectron microscopic study.
Histochemistry and cell biology137(1)37-51(2012 Jan)
Onohara Y,Yokota S

Gonadotropin-regulated testicular helicase (GRTH/DDX25): a master post-transcriptional regulator of spermatogenesis.
Advances in experimental medicine and biology70723-9(2011)
Dufau ML,Sato H,Gutti R,Tsai-Morris CH

Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a transport protein involved in gene-specific mRNA export and protein translation during spermatogenesis.
The Journal of biological chemistry281(46)35048-56(2006 Nov)
Sheng Y,Tsai-Morris CH,Gutti R,Maeda Y,Dufau ML

Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is essential for spermatid development and completion of spermatogenesis.
Proceedings of the National Academy of Sciences of the United States of America101(17)6373-8(2004 Apr)
Tsai-Morris CH,Sheng Y,Lee E,Lei KJ,Dufau ML

Cell-specific and hormone-regulated expression of gonadotropin-regulated testicular RNA helicase gene (GRTH/Ddx25) resulting from alternative utilization of translation initiation codons in the rat testis.
The Journal of biological chemistry278(30)27796-803(2003 Jul)
Sheng Y,Tsai-Morris CH,Dufau ML

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Ddx25 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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