Stk39 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stk39
Entrez Gene ID	54348
Full Name	serine threonine kinase 39
Synonyms	Spak
General protein information	Preferred Names serine threonine kinase 39 Names STE20/SPS1-related proline-alanine-rich protein kinase PS/TK PSTK1 Ste-20 related kinase pancreatic serine/threonine-protein kinase serine/threonine-protein kinase 39 ste-20-related kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q21
Summary	serine-threonine kinase from the STE20/SPS1 protein kinase family; may mediate stress-activated signals [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019362.1	NP_062235.1	STE20/SPS1-related proline-alanine-rich protein kinase
XM_008761922.2	XP_008760144.1	STE20/SPS1-related proline-alanine-rich protein kinase isoform X1
XM_017591998.1	XP_017447487.1	STE20/SPS1-related proline-alanine-rich protein kinase isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	STK39	XP_005640330.1
Mus musculus (house mouse)	Stk39	NP_058562.1
Danio rerio (zebrafish)	oxsr1b	NP_957469.2
Homo sapiens (human)	STK39	NP_037365.2
	STK39	XP_001102299.1
Rattus norvegicus (Norway rat)	Stk39	NP_062235.1
Pan troglodytes (chimpanzee)	STK39	XP_515880.3
Danio rerio (zebrafish)	oxsr1a	NP_001092217.1
Gallus gallus (chicken)	STK39	XP_004942828.1
Xenopus tropicalis (tropical clawed frog)	LOC100488948	XP_002936797.2

Gene Ontology
Bibliography

Related articles in PubMed

WNK1 kinase balances T cell adhesion versus migration in vivo.
K?chl R, Thelen F, Vanes L, Braz?o TF, Fountain K, Xie J, Huang CL, Lyck R, Stein JV, Tybulewicz VL
Nature immunology17(9)1075-83(2016 09)

Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and human lenses.
Vorontsova I, Lam L, Delpire E, Lim J, Donaldson P
Investigative ophthalmology & visual science56(1)310-21(2014 Dec)

A novel Ste20-related proline/alanine-rich kinase (SPAK)-independent pathway involving calcium-binding protein 39 (Cab39) and serine threonine kinase with no lysine member 4 (WNK4) in the activation of Na-K-Cl cotransporters.
Ponce-Coria J, Markadieu N, Austin TM, Flammang L, Rios K, Welling PA, Delpire E
The Journal of biological chemistry289(25)17680-8(2014 Jun)

The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K+-Cl- co-transporters.
de Los Heros P, Alessi DR, Gourlay R, Campbell DG, Deak M, Macartney TJ, Kahle KT, Zhang J
The Biochemical journal458(3)559-73(2014 Mar)

Negative regulation of the creatine transporter SLC6A8 by SPAK and OSR1.
Fezai M, Elvira B, Borras J, Ben-Attia M, Hoseinzadeh Z, Lang F
Kidney & blood pressure research39(6)546-54(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in addition to SPAK and OSR1, WNK4 is able to anchor itself to the N-terminal domain of NKCC1 and to promote cotransporter activation.
Title: A novel Ste20-related proline/alanine-rich kinase (SPAK)-independent pathway involving calcium-binding protein 39 (Cab39) and serine threonine kinase with no lysine member 4 (WNK4) in the activation of Na-K-Cl cotransporters.

SPAK and oxidative stress response kinase (OSR1) may be critical factors in the regulation of depolarizing GABA-mediated processes in the developing brain.
Title: Kinases SPAK and OSR1 are upregulated by estradiol and activate NKCC1 in the developing hypothalamus.

Two Ste20-like kinases that bind and phosphorylate NKCC1: Ste20-related proline-alanine-rich kinase (SPAK) and oxidative-stress response 1 (OSR1), were identified.
Title: The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons.

WNK1 and WNK4 both interacted with SPAK as well as a closely related kinase, termed oxidative stress response kinase-1 (OSR1).
Title: The WNK1 and WNK4 protein kinases that are mutated in Gordon's hypertension syndrome phosphorylate and activate SPAK and OSR1 protein kinases.

roles in phosphorylation and activation of the Na-K-Cl cotransporter (NKCC1)
Title: PASK (proline-alanine-rich STE20-related kinase), a regulatory kinase of the Na-K-Cl cotransporter (NKCC1).

The following Stk39 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stk39 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa43436	XM_008761922.2 Latest version!	Rattus norvegicus serine threonine kinase 39 (Stk39), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa60516	XM_017591998.1 Latest version!	Rattus norvegicus serine threonine kinase 39 (Stk39), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa04555	NM_019362.1 Latest version!	Rattus norvegicus serine threonine kinase 39 (Stk39), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$328.30 $469.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stk39 ( XM_008761922.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760144.1 Rattus norvegicus serine threonine kinase 39 (Stk39), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43436
Clone ID Related Accession (Same CDS sequence)	XM_008761922.2
Accession Version	XM_008761922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	STE20/SPS1-related proline-alanine-rich protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCC CTCTGGGGGT CCCTCTGTGG CGGGGAAGAC CCCCTGGTCT GCTGTCTTCC 
ACCTGCAGTT ATTCTACAGT GTCCTGGAAG GGCAGTGGAG CGACCGCCGT GGTTCAGGCA 
GCCCTGTGCA AACCCAGGCA AGAACGCGTA GCCATAAAGC GGATCAACTT GGAAAAGTGC 
CAGACGAGTA TGGATGAACT CTTAAAAGAA ATTCAAGCCA TGAGCCAGTG CAGCCATCCC 
AACGTAGTGA CTTATTATAC TTCCTTTGTG GTCAAAGATG AACTTTGGCT GGTCATGAAA 
TTACTAAGTG GAGGTTCCAT GTTGGATATC ATCAAATACA TCGTCAATCG GGGAGAGCAT 
AAGAATGGTG TCCTGGAAGA GGCAATAATC GCAACAATCC TTAAGGAGGT TTTGGAAGGA 
TTAGACTATC TGCATAGAAA TGGTCAGATC CATAGGGATT TGAAAGCTGG CAATATTCTT 
CTGGGTGAGG ATGGATCAGT ACAGATAGCA GATTTTGGAG TAAGTGCATT CTTAGCAACA 
GGGGGTGATG TCACAAGGAA TAAAGTCAGA AAAACATTTG TTGGTACCCC ATGTTGGATG 
GCCCCTGAGG TCATGGAACA GGTGAGAGGC TATGACTTCA AGGCTGACAT GTGGAGTTTT 
GGAATAACAG CCATTGAATT AGCAACGGGA GCAGCGCCTT ACCACAAATA CCCTCCAATG 
AAAGTGCTAA TGTTGACTTT GCAAAATGAC CCGCCCACTT TAGAAACCGG CGTAGAGGAT 
AAAGAAATGA TGAAAAAATA CGGCAAGTCC TTCAGAAAGT TACTTTCACT GTGTCTCCAG 
AAAGATCCTT CCAAAAGGCC CACAGCAGCA GAACTTTTAA AATGCAAATT CTTCCAGAAA 
GCCAAGAACA GAGAGTACCT GATCGAGAAG TTGCTGACAC GAACACCAGA CATAGCCCAA 
AGAGCCAAGA AGGTCAGGCG AGTTCCTGGG TCAAGCGGTC ACCTTCACAA GACTGAAGAT 
GGCGACTGGG AGTGGAGTGA TGATGAGATG GATGAGAAGA GTGAGGAGGG GAAAGCGGCT 
GCCTCTCAAG AGAAGTCACG AAGAGTAAAA GAAGAGAACC CAGAGATCTC GGTGAACGCT 
GGTGGCATCC CCGAGCAAAT ACAGTCCCTC TCCGTGCACG ACTCTCAGGG CCAACCAAAT 
GCTAATGAAG ACTACAGAGA AGGTCCTTGT GCGGTCAACC TTGTTTTAAG ATTAAGAAAC 
TCCAGAAAGG AACTTAATGA CATACGATTT GAGTTTACTC CAGGAAGAGA TACAGCAGAT 
GGTGTGTCTC AGGAGCTCTT CTCTGCTGGC TTGGTTGACG GTCATGATGT AGTTATAGTG 
GCTGCTAATT TACAGAAGAT TGTAGATGAC CCCAAAGCTT TAAAAACGTT GACATTTAAG 
TTGGCTTCTG GCTGTGATGG GGCGGAGATT CCTGACGAAG TGAAGCTGAT CGGGTTCGCC 
CAGTTGAGTG TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760144.1
CDS	299..1816
Translation	MVFPLGVPLWRGRPPGLLSSTCSYSTVSWKGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAASQEKSRRVKEENPEISVNAGGIPEQIQSLSVHDSQGQPNANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGAEIPDEVKLIGFAQLSVS

Target ORF information:

RefSeq Version	XM_008761922.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine threonine kinase 39 (Stk39), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761922.2

ORF Insert Sequence:

ATGGTGTTCC CTCTGGGGGT CCCTCTGTGG CGGGGAAGAC CCCCTGGTCT GCTGTCTTCC 
ACCTGCAGTT ATTCTACAGT GTCCTGGAAG GGCAGTGGAG CGACCGCCGT GGTTCAGGCA 
GCCCTGTGCA AACCCAGGCA AGAACGCGTA GCCATAAAGC GGATCAACTT GGAAAAGTGC 
CAGACGAGTA TGGATGAACT CTTAAAAGAA ATTCAAGCCA TGAGCCAGTG CAGCCATCCC 
AACGTAGTGA CTTATTATAC TTCCTTTGTG GTCAAAGATG AACTTTGGCT GGTCATGAAA 
TTACTAAGTG GAGGTTCCAT GTTGGATATC ATCAAATACA TCGTCAATCG GGGAGAGCAT 
AAGAATGGTG TCCTGGAAGA GGCAATAATC GCAACAATCC TTAAGGAGGT TTTGGAAGGA 
TTAGACTATC TGCATAGAAA TGGTCAGATC CATAGGGATT TGAAAGCTGG CAATATTCTT 
CTGGGTGAGG ATGGATCAGT ACAGATAGCA GATTTTGGAG TAAGTGCATT CTTAGCAACA 
GGGGGTGATG TCACAAGGAA TAAAGTCAGA AAAACATTTG TTGGTACCCC ATGTTGGATG 
GCCCCTGAGG TCATGGAACA GGTGAGAGGC TATGACTTCA AGGCTGACAT GTGGAGTTTT 
GGAATAACAG CCATTGAATT AGCAACGGGA GCAGCGCCTT ACCACAAATA CCCTCCAATG 
AAAGTGCTAA TGTTGACTTT GCAAAATGAC CCGCCCACTT TAGAAACCGG CGTAGAGGAT 
AAAGAAATGA TGAAAAAATA CGGCAAGTCC TTCAGAAAGT TACTTTCACT GTGTCTCCAG 
AAAGATCCTT CCAAAAGGCC CACAGCAGCA GAACTTTTAA AATGCAAATT CTTCCAGAAA 
GCCAAGAACA GAGAGTACCT GATCGAGAAG TTGCTGACAC GAACACCAGA CATAGCCCAA 
AGAGCCAAGA AGGTCAGGCG AGTTCCTGGG TCAAGCGGTC ACCTTCACAA GACTGAAGAT 
GGCGACTGGG AGTGGAGTGA TGATGAGATG GATGAGAAGA GTGAGGAGGG GAAAGCGGCT 
GCCTCTCAAG AGAAGTCACG AAGAGTAAAA GAAGAGAACC CAGAGATCTC GGTGAACGCT 
GGTGGCATCC CCGAGCAAAT ACAGTCCCTC TCCGTGCACG ACTCTCAGGG CCAACCAAAT 
GCTAATGAAG ACTACAGAGA AGGTCCTTGT GCGGTCAACC TTGTTTTAAG ATTAAGAAAC 
TCCAGAAAGG AACTTAATGA CATACGATTT GAGTTTACTC CAGGAAGAGA TACAGCAGAT 
GGTGTGTCTC AGGAGCTCTT CTCTGCTGGC TTGGTTGACG GTCATGATGT AGTTATAGTG 
GCTGCTAATT TACAGAAGAT TGTAGATGAC CCCAAAGCTT TAAAAACGTT GACATTTAAG 
TTGGCTTCTG GCTGTGATGG GGCGGAGATT CCTGACGAAG TGAAGCTGAT CGGGTTCGCC 
CAGTTGAGTG TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stk39 ( XM_017591998.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447487.1 Rattus norvegicus serine threonine kinase 39 (Stk39), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60516
Clone ID Related Accession (Same CDS sequence)	XM_017591998.1
Accession Version	XM_017591998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	STE20/SPS1-related proline-alanine-rich protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCCA GTTTCATTTC AGAGCAGCAC AGCGATTACA TTAGGCAAGG TGCGAAAGGG 
CTCCTACCAG GCAGTGGAGC GACCGCCGTG GTTCAGGCAG CCCTGTGCAA ACCCAGGCAA 
GAACGCGTAG CCATAAAGCG GATCAACTTG GAAAAGTGCC AGACGAGTAT GGATGAACTC 
TTAAAAGAAA TTCAAGCCAT GAGCCAGTGC AGCCATCCCA ACGTAGTGAC TTATTATACT 
TCCTTTGTGG TCAAAGATGA ACTTTGGCTG GTCATGAAAT TACTAAGTGG AGGTTCCATG 
TTGGATATCA TCAAATACAT CGTCAATCGG GGAGAGCATA AGAATGGTGT CCTGGAAGAG 
GCAATAATCG CAACAATCCT TAAGGAGGTT TTGGAAGGAT TAGACTATCT GCATAGAAAT 
GGTCAGATCC ATAGGGATTT GAAAGCTGGC AATATTCTTC TGGGTGAGGA TGGATCAGTA 
CAGATAGCAG ATTTTGGAGT AAGTGCATTC TTAGCAACAG GGGGTGATGT CACAAGGAAT 
AAAGTCAGAA AAACATTTGT TGGTACCCCA TGTTGGATGG CCCCTGAGGT CATGGAACAG 
GTGAGAGGCT ATGACTTCAA GGCTGACATG TGGAGTTTTG GAATAACAGC CATTGAATTA 
GCAACGGGAG CAGCGCCTTA CCACAAATAC CCTCCAATGA AAGTGCTAAT GTTGACTTTG 
CAAAATGACC CGCCCACTTT AGAAACCGGC GTAGAGGATA AAGAAATGAT GAAAAAATAC 
GGCAAGTCCT TCAGAAAGTT ACTTTCACTG TGTCTCCAGA AAGATCCTTC CAAAAGGCCC 
ACAGCAGCAG AACTTTTAAA ATGCAAATTC TTCCAGAAAG CCAAGAACAG AGAGTACCTG 
ATCGAGAAGT TGCTGACACG AACACCAGAC ATAGCCCAAA GAGCCAAGAA GGTCAGGCGA 
GTTCCTGGGT CAAGCGGTCA CCTTCACAAG ACTGAAGATG GCGACTGGGA GTGGAGTGAT 
GATGAGATGG ATGAGAAGAG TGAGGAGGGG AAAGCGGCTG CCTCTCAAGA GAAGTCACGA 
AGAGTAAAAG AAGAGAACCC AGAGATCTCG GTGAACGCTG GTGGCATCCC CGAGCAAATA 
CAGTCCCTCT CCGTGCACGA CTCTCAGGGC CAACCAAATG CTAATGAAGA CTACAGAGAA 
GGTCCTTGTG CGGTCAACCT TGTTTTAAGA TTAAGAAACT CCAGAAAGGA ACTTAATGAC 
ATACGATTTG AGTTTACTCC AGGAAGAGAT ACAGCAGATG GTGTGTCTCA GGAGCTCTTC 
TCTGCTGGCT TGGTTGACGG TCATGATGTA GTTATAGTGG CTGCTAATTT ACAGAAGATT 
GTAGATGACC CCAAAGCTTT AAAAACGTTG ACATTTAAGT TGGCTTCTGG CTGTGATGGG 
GCGGAGATTC CTGACGAAGT GAAGCTGATC GGGTTCGCCC AGTTGAGTGT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447487.1
CDS	16..1512
Translation	MESSFISEQHSDYIRQGAKGLLPGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAASQEKSRRVKEENPEISVNAGGIPEQIQSLSVHDSQGQPNANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGAEIPDEVKLIGFAQLSVS

Target ORF information:

RefSeq Version	XM_017591998.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine threonine kinase 39 (Stk39), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591998.1

ORF Insert Sequence:

ATGGAGTCCA GTTTCATTTC AGAGCAGCAC AGCGATTACA TTAGGCAAGG TGCGAAAGGG 
CTCCTACCAG GCAGTGGAGC GACCGCCGTG GTTCAGGCAG CCCTGTGCAA ACCCAGGCAA 
GAACGCGTAG CCATAAAGCG GATCAACTTG GAAAAGTGCC AGACGAGTAT GGATGAACTC 
TTAAAAGAAA TTCAAGCCAT GAGCCAGTGC AGCCATCCCA ACGTAGTGAC TTATTATACT 
TCCTTTGTGG TCAAAGATGA ACTTTGGCTG GTCATGAAAT TACTAAGTGG AGGTTCCATG 
TTGGATATCA TCAAATACAT CGTCAATCGG GGAGAGCATA AGAATGGTGT CCTGGAAGAG 
GCAATAATCG CAACAATCCT TAAGGAGGTT TTGGAAGGAT TAGACTATCT GCATAGAAAT 
GGTCAGATCC ATAGGGATTT GAAAGCTGGC AATATTCTTC TGGGTGAGGA TGGATCAGTA 
CAGATAGCAG ATTTTGGAGT AAGTGCATTC TTAGCAACAG GGGGTGATGT CACAAGGAAT 
AAAGTCAGAA AAACATTTGT TGGTACCCCA TGTTGGATGG CCCCTGAGGT CATGGAACAG 
GTGAGAGGCT ATGACTTCAA GGCTGACATG TGGAGTTTTG GAATAACAGC CATTGAATTA 
GCAACGGGAG CAGCGCCTTA CCACAAATAC CCTCCAATGA AAGTGCTAAT GTTGACTTTG 
CAAAATGACC CGCCCACTTT AGAAACCGGC GTAGAGGATA AAGAAATGAT GAAAAAATAC 
GGCAAGTCCT TCAGAAAGTT ACTTTCACTG TGTCTCCAGA AAGATCCTTC CAAAAGGCCC 
ACAGCAGCAG AACTTTTAAA ATGCAAATTC TTCCAGAAAG CCAAGAACAG AGAGTACCTG 
ATCGAGAAGT TGCTGACACG AACACCAGAC ATAGCCCAAA GAGCCAAGAA GGTCAGGCGA 
GTTCCTGGGT CAAGCGGTCA CCTTCACAAG ACTGAAGATG GCGACTGGGA GTGGAGTGAT 
GATGAGATGG ATGAGAAGAG TGAGGAGGGG AAAGCGGCTG CCTCTCAAGA GAAGTCACGA 
AGAGTAAAAG AAGAGAACCC AGAGATCTCG GTGAACGCTG GTGGCATCCC CGAGCAAATA 
CAGTCCCTCT CCGTGCACGA CTCTCAGGGC CAACCAAATG CTAATGAAGA CTACAGAGAA 
GGTCCTTGTG CGGTCAACCT TGTTTTAAGA TTAAGAAACT CCAGAAAGGA ACTTAATGAC 
ATACGATTTG AGTTTACTCC AGGAAGAGAT ACAGCAGATG GTGTGTCTCA GGAGCTCTTC 
TCTGCTGGCT TGGTTGACGG TCATGATGTA GTTATAGTGG CTGCTAATTT ACAGAAGATT 
GTAGATGACC CCAAAGCTTT AAAAACGTTG ACATTTAAGT TGGCTTCTGG CTGTGATGGG 
GCGGAGATTC CTGACGAAGT GAAGCTGATC GGGTTCGCCC AGTTGAGTGT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stk39 ( NM_019362.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062235.1 Rattus norvegicus serine threonine kinase 39 (Stk39), mRNA.

Price & Availability↑

CloneID	ORa04555
Clone ID Related Accession (Same CDS sequence)	NM_019362.1
Accession Version	NM_019362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	STE20/SPS1-related proline-alanine-rich protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D88190.1. On Jun 25, 2006 this sequence version replaced XM_001057901.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D88190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGAGC CGAGCGGCTC GCCCGTGCAC GTCCAGCTTC CCCAGCAGGC GGCCCCGGTG 
ACAGCCGCGG CGGCGGCTCC GGCGGCCGCG ACATCAGCAC CGGCCCCGGC CCCGGCCCCG 
GCGGCGCCCG CAGCCCCGGC CCCGGCTCCA GCTGCGGCTC CAGCCCCGGC CCCGGCAGCT 
CAGGCGGTCG GCTGGCCCAT CTGCAGGGAC GCGTACGAGC TCCAGGAGGT TATCGGCAGT 
GGAGCGACCG CCGTGGTTCA GGCAGCCCTG TGCAAACCCA GGCAAGAACG CGTAGCCATA 
AAGCGGATCA ACTTGGAAAA GTGCCAGACG AGTATGGATG AACTCTTAAA AGAAATTCAA 
GCCATGAGCC AGTGCAGCCA TCCCAACGTA GTGACTTATT ATACTTCCTT TGTGGTCAAA 
GATGAACTTT GGCTGGTCAT GAAATTACTA AGTGGAGGTT CCATGTTGGA TATCATCAAA 
TACATCGTCA ATCGGGGAGA GCATAAGAAT GGTGTCCTGG AAGAGGCAAT AATCGCAACA 
ATCCTTAAGG AGGTTTTGGA AGGATTAGAC TATCTGCATA GAAATGGTCA GATCCATAGG 
GATTTGAAAG CTGGCAATAT TCTTCTGGGT GAGGATGGAT CAGTACAGAT AGCAGATTTT 
GGAGTAAGTG CATTCTTAGC AACAGGGGGT GATGTCACAA GGAATAAAGT CAGAAAAACA 
TTTGTTGGTA CCCCATGTTG GATGGCCCCT GAGGTCATGG AACAGGTGAG AGGCTATGAC 
TTCAAGGCTG ACATGTGGAG TTTTGGAATA ACAGCCATTG AATTAGCAAC GGGAGCAGCG 
CCTTACCACA AATACCCTCC AATGAAAGTG CTAATGTTGA CTTTGCAAAA TGACCCGCCC 
ACTTTAGAAA CCGGCGTAGA GGATAAAGAA ATGATGAAAA AATACGGCAA GTCCTTCAGA 
AAGTTACTTT CACTGTGTCT CCAGAAAGAT CCTTCCAAAA GGCCCACAGC AGCAGAACTT 
TTAAAATGCA AATTCTTCCA GAAAGCCAAG AACAGAGAGT ACCTGATCGA GAAGTTGCTG 
ACACGAACAC CAGACATAGC CCAAAGAGCC AAGAAGGTCA GGCGAGTTCC TGGGTCAAGC 
GGTCACCTTC ACAAGACTGA AGATGGCGAC TGGGAGTGGA GTGATGATGA GATGGATGAG 
AAGAGTGAGG AGGGGAAAGC GGCTGCCTCT CAAGAGAAGT CACGAAGAGT AAAAGAAGAG 
AACCCAGAGA TCTCGGTGAA CGCTGGTGGC ATCCCCGAGC AAATACAGTC CCTCTCCGTG 
CACGACTCTC AGGGCCAACC AAATGCTAAT GAAGACTACA GAGAAGGTCC TTGTGCGGTC 
AACCTTGTTT TAAGATTAAG AAACTCCAGA AAGGAACTTA ATGACATACG ATTTGAGTTT 
ACTCCAGGAA GAGATACAGC AGATGGTGTG TCTCAGGAGC TCTTCTCTGC TGGCTTGGTT 
GACGGTCATG ATGTAGTTAT AGTGGCTGCT AATTTACAGA AGATTGTAGA TGACCCCAAA 
GCTTTAAAAA CGTTGACATT TAAGTTGGCT TCTGGCTGTG ATGGGGCGGA GATTCCTGAC 
GAAGTGAAGC TGATCGGGTT CGCCCAGTTG AGTGTGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062235.1
CDS	417..2078
Translation	MAEPSGSPVHVQLPQQAAPVTAAAAAPAAATSAPAPAPAPAAPAAPAPAPAAAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAASQEKSRRVKEENPEISVNAGGIPEQIQSLSVHDSQGQPNANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGAEIPDEVKLIGFAQLSVS

Target ORF information:

RefSeq Version	NM_019362.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine threonine kinase 39 (Stk39), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019362.1

ORF Insert Sequence:

ATGGCGGAGC CGAGCGGCTC GCCCGTGCAC GTCCAGCTTC CCCAGCAGGC GGCCCCGGTG 
ACAGCCGCGG CGGCGGCTCC GGCGGCCGCG ACATCAGCAC CGGCCCCGGC CCCGGCCCCG 
GCGGCGCCCG CAGCCCCGGC CCCGGCTCCA GCTGCGGCTC CAGCCCCGGC CCCGGCAGCT 
CAGGCGGTCG GCTGGCCCAT CTGCAGGGAC GCGTACGAGC TCCAGGAGGT TATCGGCAGT 
GGAGCGACCG CCGTGGTTCA GGCAGCCCTG TGCAAACCCA GGCAAGAACG CGTAGCCATA 
AAGCGGATCA ACTTGGAAAA GTGCCAGACG AGTATGGATG AACTCTTAAA AGAAATTCAA 
GCCATGAGCC AGTGCAGCCA TCCCAACGTA GTGACTTATT ATACTTCCTT TGTGGTCAAA 
GATGAACTTT GGCTGGTCAT GAAATTACTA AGTGGAGGTT CCATGTTGGA TATCATCAAA 
TACATCGTCA ATCGGGGAGA GCATAAGAAT GGTGTCCTGG AAGAGGCAAT AATCGCAACA 
ATCCTTAAGG AGGTTTTGGA AGGATTAGAC TATCTGCATA GAAATGGTCA GATCCATAGG 
GATTTGAAAG CTGGCAATAT TCTTCTGGGT GAGGATGGAT CAGTACAGAT AGCAGATTTT 
GGAGTAAGTG CATTCTTAGC AACAGGGGGT GATGTCACAA GGAATAAAGT CAGAAAAACA 
TTTGTTGGTA CCCCATGTTG GATGGCCCCT GAGGTCATGG AACAGGTGAG AGGCTATGAC 
TTCAAGGCTG ACATGTGGAG TTTTGGAATA ACAGCCATTG AATTAGCAAC GGGAGCAGCG 
CCTTACCACA AATACCCTCC AATGAAAGTG CTAATGTTGA CTTTGCAAAA TGACCCGCCC 
ACTTTAGAAA CCGGCGTAGA GGATAAAGAA ATGATGAAAA AATACGGCAA GTCCTTCAGA 
AAGTTACTTT CACTGTGTCT CCAGAAAGAT CCTTCCAAAA GGCCCACAGC AGCAGAACTT 
TTAAAATGCA AATTCTTCCA GAAAGCCAAG AACAGAGAGT ACCTGATCGA GAAGTTGCTG 
ACACGAACAC CAGACATAGC CCAAAGAGCC AAGAAGGTCA GGCGAGTTCC TGGGTCAAGC 
GGTCACCTTC ACAAGACTGA AGATGGCGAC TGGGAGTGGA GTGATGATGA GATGGATGAG 
AAGAGTGAGG AGGGGAAAGC GGCTGCCTCT CAAGAGAAGT CACGAAGAGT AAAAGAAGAG 
AACCCAGAGA TCTCGGTGAA CGCTGGTGGC ATCCCCGAGC AAATACAGTC CCTCTCCGTG 
CACGACTCTC AGGGCCAACC AAATGCTAAT GAAGACTACA GAGAAGGTCC TTGTGCGGTC 
AACCTTGTTT TAAGATTAAG AAACTCCAGA AAGGAACTTA ATGACATACG ATTTGAGTTT 
ACTCCAGGAA GAGATACAGC AGATGGTGTG TCTCAGGAGC TCTTCTCTGC TGGCTTGGTT 
GACGGTCATG ATGTAGTTAT AGTGGCTGCT AATTTACAGA AGATTGTAGA TGACCCCAAA 
GCTTTAAAAA CGTTGACATT TAAGTTGGCT TCTGGCTGTG ATGGGGCGGA GATTCCTGAC 
GAAGTGAAGC TGATCGGGTT CGCCCAGTTG AGTGTGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WNK1 kinase balances T cell adhesion versus migration in vivo.
Nature immunology17(9)1075-83(2016 09)
K?chl R,Thelen F,Vanes L,Braz?o TF,Fountain K,Xie J,Huang CL,Lyck R,Stein JV,Tybulewicz VL

Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and human lenses.
Investigative ophthalmology & visual science56(1)310-21(2014 Dec)
Vorontsova I,Lam L,Delpire E,Lim J,Donaldson P

A novel Ste20-related proline/alanine-rich kinase (SPAK)-independent pathway involving calcium-binding protein 39 (Cab39) and serine threonine kinase with no lysine member 4 (WNK4) in the activation of Na-K-Cl cotransporters.
The Journal of biological chemistry289(25)17680-8(2014 Jun)
Ponce-Coria J,Markadieu N,Austin TM,Flammang L,Rios K,Welling PA,Delpire E

The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K+-Cl- co-transporters.
The Biochemical journal458(3)559-73(2014 Mar)
de Los Heros P,Alessi DR,Gourlay R,Campbell DG,Deak M,Macartney TJ,Kahle KT,Zhang J

Negative regulation of the creatine transporter SLC6A8 by SPAK and OSR1.
Kidney & blood pressure research39(6)546-54(2014)
Fezai M,Elvira B,Borras J,Ben-Attia M,Hoseinzadeh Z,Lang F

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Stk39 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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