Mcpt4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcpt4
Entrez Gene ID	54270
Full Name	mast cell protease 4
General protein information	Preferred Names mast cell protease 4 Names mast cell protease 4 mast cell protease IV rMCP-4 rMCP-IV
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p13
Summary	mast cell protease that acts as a beta-chymase; has a strong preference for bulky/aromatic amino acid residues in both the P1 and P2 positions [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019321.2	NP_062194.1	mast cell protease 4 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mcpt4	NP_062194.1
Rattus norvegicus (Norway rat)	Mcpt3	NP_001163937.1
Rattus norvegicus (Norway rat)	Mcpt4l1	XP_006252101.1

Gene Ontology
Bibliography

Related articles in PubMed

Rat mast cell protease 4 is a beta-chymase with unusually stringent substrate recognition profile.
Karlson U, Pejler G, Froman G, Hellman L
The Journal of biological chemistry277(21)18579-85(2002 May)

Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations.
L?tzelschwab C, Pejler G, Aveskogh M, Hellman L
The Journal of experimental medicine185(1)13-29(1997 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

investigation of substrate recognition profile
Title: Rat mast cell protease 4 is a beta-chymase with unusually stringent substrate recognition profile.

The following Mcpt4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcpt4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04409	NM_019321.2 Latest version!	Rattus norvegicus mast cell protease 4 (Mcpt4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcpt4 ( NM_019321.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062194.1 Rattus norvegicus mast cell protease 4 (Mcpt4), mRNA.

Price & Availability↑

CloneID	ORa04409
Clone ID Related Accession (Same CDS sequence)	NM_019321.2
Accession Version	NM_019321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Rattus norvegicus(Norway rat)
Product	mast cell protease 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U67907.1. On Feb 21, 2013 this sequence version replaced NM_019321.1. ##Evidence-Data-START## Transcript exon combination :: U67907.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689603, SAMEA2689605 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGCCC TGCTATTCCT GATGGCTCTT CTCTTGCCTT CTGGAGCTGG AGCTGAGGAG 
ATTATCGGTG GTGTGGAGTC TATTCCTCAC TCCCGCCCTT ACATGGCCCT TCTGAAGATC 
GTCACTGAAG AAGGTCATGT GACCTTCTGT GGTGGGTTTC TCATAAGCCT TCAATTTGTG 
CTGACTGCTG CACACTGTCA TGGAAGGGAA ATCACTGTCA CCCTCGGAGC TCATGACATG 
AGCAAGAGAG AATCCACACA ACAAAAGATA AAAGTTGTAA AACAAATCTT TCCTCTAAAG 
TACAATTTAT TTTCCAATTT CCGTGACATC ATGTTACTGA AGCTTGAACA GAAAGCAGTG 
TTGACTCCGT CTGTGAATGT AATCCCCCTG CCTCAGTCCT CTGACATTAT CAAGCCTGGG 
ACAATGTGCT TGGCAGCTGG ATGGGGACAA ACTGGAGTGA AAGAACCGAA CTCAAATACA 
CTGAGGGAGG TCATGCTGAG AATCATGGAA ATGAAGGCCT GTAAGGACTA TAGGCATTAT 
GACAATAGAT TCCAGATCTG CGTGGGGATT CCTCAAATGT TGAAATTAGC ATACAAGGGA 
GACTCTGGAG GACCTCTAGT GTGTGCTGGA GTGGCCCATG GTATTGTATC TCATGGCCCT 
GGAAGAGGAA TCCCCCCTAT AATCTTCACC CGAATCTCCT CATATGTGTC CTGGATTAAT 
AGAGTCATAA GGGGAAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062194.1
CDS	14..754
Translation	MKALLFLMALLLPSGAGAEEIIGGVESIPHSRPYMALLKIVTEEGHVTFCGGFLISLQFVLTAAHCHGREITVTLGAHDMSKRESTQQKIKVVKQIFPLKYNLFSNFRDIMLLKLEQKAVLTPSVNVIPLPQSSDIIKPGTMCLAAGWGQTGVKEPNSNTLREVMLRIMEMKACKDYRHYDNRFQICVGIPQMLKLAYKGDSGGPLVCAGVAHGIVSHGPGRGIPPIIFTRISSYVSWINRVIRGN

Target ORF information:

RefSeq Version	NM_019321.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mast cell protease 4 (Mcpt4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019321.2

ORF Insert Sequence:

ATGAAGGCCC TGCTATTCCT GATGGCTCTT CTCTTGCCTT CTGGAGCTGG AGCTGAGGAG 
ATTATCGGTG GTGTGGAGTC TATTCCTCAC TCCCGCCCTT ACATGGCCCT TCTGAAGATC 
GTCACTGAAG AAGGTCATGT GACCTTCTGT GGTGGGTTTC TCATAAGCCT TCAATTTGTG 
CTGACTGCTG CACACTGTCA TGGAAGGGAA ATCACTGTCA CCCTCGGAGC TCATGACATG 
AGCAAGAGAG AATCCACACA ACAAAAGATA AAAGTTGTAA AACAAATCTT TCCTCTAAAG 
TACAATTTAT TTTCCAATTT CCGTGACATC ATGTTACTGA AGCTTGAACA GAAAGCAGTG 
TTGACTCCGT CTGTGAATGT AATCCCCCTG CCTCAGTCCT CTGACATTAT CAAGCCTGGG 
ACAATGTGCT TGGCAGCTGG ATGGGGACAA ACTGGAGTGA AAGAACCGAA CTCAAATACA 
CTGAGGGAGG TCATGCTGAG AATCATGGAA ATGAAGGCCT GTAAGGACTA TAGGCATTAT 
GACAATAGAT TCCAGATCTG CGTGGGGATT CCTCAAATGT TGAAATTAGC ATACAAGGGA 
GACTCTGGAG GACCTCTAGT GTGTGCTGGA GTGGCCCATG GTATTGTATC TCATGGCCCT 
GGAAGAGGAA TCCCCCCTAT AATCTTCACC CGAATCTCCT CATATGTGTC CTGGATTAAT 
AGAGTCATAA GGGGAAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rat mast cell protease 4 is a beta-chymase with unusually stringent substrate recognition profile.
The Journal of biological chemistry277(21)18579-85(2002 May)
Karlson U,Pejler G,Froman G,Hellman L

Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations.
The Journal of experimental medicine185(1)13-29(1997 Jan)
L?tzelschwab C,Pejler G,Aveskogh M,Hellman L