Cpa3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpa3
Entrez Gene ID	54242
Full Name	carboxypeptidase A3
General protein information	Preferred Names carboxypeptidase A3 Names mast cell carboxypeptidase A R-CPA RMC-CP carboxypeptidase A3, mast cell
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q24
Summary	protease expressed in mast cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019300.1	NP_062173.1	mast cell carboxypeptidase A precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CPA3	NP_001861.2
Bos taurus (cattle)	CPA3	NP_001179589.1
Magnaporthe oryzae (rice blast fungus)	MGG_06906	XP_003709640.1
	CPA3	XP_001110081.2
Canis lupus familiaris (dog)	CPA3	XP_542828.2
Kluyveromyces lactis	KLLA0F27929g	XP_456322.1
Neurospora crassa	NCU01464	XP_956741.1
Pan troglodytes (chimpanzee)	CPA3	XP_526465.2
Rattus norvegicus (Norway rat)	Cpa3	NP_062173.1
Saccharomyces cerevisiae (baker's yeast)	ECM14	NP_012000.1
Xenopus tropicalis (tropical clawed frog)	cpb1	XP_002937384.2
Mus musculus (house mouse)	Cpa3	NP_031779.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013181	XP_003437177.1
Eremothecium gossypii	AGOS_AFR045W	NP_985592.1
Schizosaccharomyces pombe (fission yeast)	SPBC337.07c	NP_595408.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia-reperfusion injury in rats after ischemic preconditioning.
Xing D, Zhang R, Li S, Huang P, Luo C, Hei Z, Xia Z, Gan X
The Journal of surgical research192(1)177-86(2014 Nov)

Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis.
Taketomi Y, Ueno N, Kojima T, Sato H, Murase R, Yamamoto K, Tanaka S, Sakanaka M, Nakamura M, Nishito Y, Kawana M, Kambe N, Ikeda K, Taguchi R, Nakamizo S, Kabashima K, Gelb MH, Arita M, Yokomizo T, Nakamura M, Watanabe K, Hirai H, Nakamura M, Okayama Y, Ra C, Aritake K, Urade Y, Morimoto K, Sugimoto Y, Shimizu T, Narumiya S, Hara S, Murakami M
Nature immunology14(6)554-63(2013 Jun)

Cooperation between mast cell carboxypeptidase A and the chymase mouse mast cell protease 4 in the formation and degradation of angiotensin II.
Lundequist A, Tchougounova E, Abrink M, Pejler G
The Journal of biological chemistry279(31)32339-44(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that ischemic preconditioning protected against reperfusion injury via the mast cell degranulation-mediated release of MC-CPA.
Title: Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia-reperfusion injury in rats after ischemic preconditioning.

The following Cpa3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpa3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04333	NM_019300.1 Latest version!	Rattus norvegicus carboxypeptidase A3 (Cpa3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpa3 ( NM_019300.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062173.1 Rattus norvegicus carboxypeptidase A3 (Cpa3), mRNA.

Price & Availability↑

CloneID	ORa04333
Clone ID Related Accession (Same CDS sequence)	NM_019300.1
Accession Version	NM_019300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	mast cell carboxypeptidase A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM101110.1 and U67914.1. On or before Nov 21, 2008 this sequence version replaced XM_342219.3, XM_001056274.1, XM_001056211.1. Sequence Note: This RefSeq record was created from transcripts from mixed strains because no data from the same strain was available for the full length of the gene. The extent of the transcript record is supported by alignment data. ##Evidence-Data-START## Transcript exon combination :: U67914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906357 [ECO:0000348] ##Evidence-Data-END##

ATGAGGTCCT TCTTGCTTAT GGGGGTCATC TATTCCACAC TTGCAATTGC TCCTGTTCAG 
TTTGACAGGG AGAAGGTATT CCGTGTGAAA CTCCAGGATG AAAAGCAAGC AAGTATCTTG 
AAGAACTTGA CCCAAACCAT TGAGCTTGAC TTCTGGTATC CAGATGCTAT CCATGATATT 
GCTGTTAATA TGACTGTGGA TTTCCGAGTT ACTGAGAAGG AATCTCAGAC TATCCAGTCT 
ACCTTGGAAC AACATAAAAT GGACTATGAA ATTCTGATAA ATGACCTACA AGAAGAGATA 
GACAAACAGT TTGATGTGAA AGAGGAAATC GCAGGCAGGC ACAGTTATGC AAAATACAAT 
GACTGGAACA AGATTGTTTC TTGGACTGAA AAAATGGTAG AAAAACATCC TGAAATGGTT 
TCAAGGATTA AAATTGGATC AACTGTTGAA GATAATCCAT TATATGTTCT CAAGATTGGG 
AGAAAAGATG GAGAAAGAAA GGCTATCTTT ATGGACTGTG GCATCCATGC TCGTGAATGG 
GTTTCCCCAG CTTTCTGTCA GTGGTTTGTC TATCAGGCAG CCAAGAGTTA TGGAAAAAAT 
AAAATCATGA CCAAACTTTT GGACCGGATG AATTTTTATG TTCTGCCCGT ATTCAATGTA 
GATGGATACA TTTGGTCATG GACAAAGGAT CGAATGTGGA GAAAAAACCG TTCCAAGAAC 
CCAAATTCAA CCTGCATTGG CACTGACCTC AACAGGAACT TCGATGTCTC ATGGGACTCT 
TCCCCAAACA CCGACAACCC ATGCCTCAGT GTCTACAGGG GACCTGCACC AGAGTCCGAG 
AAAGAGACAA AAGCTGTCAC TAACTTCATT CGAAGTCATC TGAACTCAAT CAAGGCTTAT 
ATCACCTTCC ATTCCTACTC TCAGATGCTG TTATTTCCTT ATGGCTACAC AATCAAACTG 
CCACCCAACC ACCAGGACCT GCTGAAGGTT GCGAGGATTG CCACTGATGT TCTCTCAAGT 
AGATATGAAA CCCGTTATAT CTACGGCCCA ATAGCATCCA CAATTTACAA GACATCAGGT 
TCTTCTTTAG ACTGGGCTTA TGACCTGGGC ATCAAACACA CATTTGCCTT TGAGCTTCGT 
GATAAAGGAA AATCTGGTTT CCTTCTTCCA GAATCTCGGA TAAAGCCAAC ATGTAAAGAA 
ACCATGCTTT CTGTCAAATT TATTGCCAAG TATATCCTCA AGCATACTTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062173.1
CDS	2..1255
Translation	MRSFLLMGVIYSTLAIAPVQFDREKVFRVKLQDEKQASILKNLTQTIELDFWYPDAIHDIAVNMTVDFRVTEKESQTIQSTLEQHKMDYEILINDLQEEIDKQFDVKEEIAGRHSYAKYNDWNKIVSWTEKMVEKHPEMVSRIKIGSTVEDNPLYVLKIGRKDGERKAIFMDCGIHAREWVSPAFCQWFVYQAAKSYGKNKIMTKLLDRMNFYVLPVFNVDGYIWSWTKDRMWRKNRSKNPNSTCIGTDLNRNFDVSWDSSPNTDNPCLSVYRGPAPESEKETKAVTNFIRSHLNSIKAYITFHSYSQMLLFPYGYTIKLPPNHQDLLKVARIATDVLSSRYETRYIYGPIASTIYKTSGSSLDWAYDLGIKHTFAFELRDKGKSGFLLPESRIKPTCKETMLSVKFIAKYILKHTS

Target ORF information:

RefSeq Version	NM_019300.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carboxypeptidase A3 (Cpa3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019300.1

ORF Insert Sequence:

ATGAGGTCCT TCTTGCTTAT GGGGGTCATC TATTCCACAC TTGCAATTGC TCCTGTTCAG 
TTTGACAGGG AGAAGGTATT CCGTGTGAAA CTCCAGGATG AAAAGCAAGC AAGTATCTTG 
AAGAACTTGA CCCAAACCAT TGAGCTTGAC TTCTGGTATC CAGATGCTAT CCATGATATT 
GCTGTTAATA TGACTGTGGA TTTCCGAGTT ACTGAGAAGG AATCTCAGAC TATCCAGTCT 
ACCTTGGAAC AACATAAAAT GGACTATGAA ATTCTGATAA ATGACCTACA AGAAGAGATA 
GACAAACAGT TTGATGTGAA AGAGGAAATC GCAGGCAGGC ACAGTTATGC AAAATACAAT 
GACTGGAACA AGATTGTTTC TTGGACTGAA AAAATGGTAG AAAAACATCC TGAAATGGTT 
TCAAGGATTA AAATTGGATC AACTGTTGAA GATAATCCAT TATATGTTCT CAAGATTGGG 
AGAAAAGATG GAGAAAGAAA GGCTATCTTT ATGGACTGTG GCATCCATGC TCGTGAATGG 
GTTTCCCCAG CTTTCTGTCA GTGGTTTGTC TATCAGGCAG CCAAGAGTTA TGGAAAAAAT 
AAAATCATGA CCAAACTTTT GGACCGGATG AATTTTTATG TTCTGCCCGT ATTCAATGTA 
GATGGATACA TTTGGTCATG GACAAAGGAT CGAATGTGGA GAAAAAACCG TTCCAAGAAC 
CCAAATTCAA CCTGCATTGG CACTGACCTC AACAGGAACT TCGATGTCTC ATGGGACTCT 
TCCCCAAACA CCGACAACCC ATGCCTCAGT GTCTACAGGG GACCTGCACC AGAGTCCGAG 
AAAGAGACAA AAGCTGTCAC TAACTTCATT CGAAGTCATC TGAACTCAAT CAAGGCTTAT 
ATCACCTTCC ATTCCTACTC TCAGATGCTG TTATTTCCTT ATGGCTACAC AATCAAACTG 
CCACCCAACC ACCAGGACCT GCTGAAGGTT GCGAGGATTG CCACTGATGT TCTCTCAAGT 
AGATATGAAA CCCGTTATAT CTACGGCCCA ATAGCATCCA CAATTTACAA GACATCAGGT 
TCTTCTTTAG ACTGGGCTTA TGACCTGGGC ATCAAACACA CATTTGCCTT TGAGCTTCGT 
GATAAAGGAA AATCTGGTTT CCTTCTTCCA GAATCTCGGA TAAAGCCAAC ATGTAAAGAA 
ACCATGCTTT CTGTCAAATT TATTGCCAAG TATATCCTCA AGCATACTTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia-reperfusion injury in rats after ischemic preconditioning.
The Journal of surgical research192(1)177-86(2014 Nov)
Xing D,Zhang R,Li S,Huang P,Luo C,Hei Z,Xia Z,Gan X

Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis.
Nature immunology14(6)554-63(2013 Jun)
Taketomi Y,Ueno N,Kojima T,Sato H,Murase R,Yamamoto K,Tanaka S,Sakanaka M,Nakamura M,Nishito Y,Kawana M,Kambe N,Ikeda K,Taguchi R,Nakamizo S,Kabashima K,Gelb MH,Arita M,Yokomizo T,Nakamura M,Watanabe K,Hirai H,Nakamura M,Okayama Y,Ra C,Aritake K,Urade Y,Morimoto K,Sugimoto Y,Shimizu T,Narumiya S,Hara S,Murakami M

Cooperation between mast cell carboxypeptidase A and the chymase mouse mast cell protease 4 in the formation and degradation of angiotensin II.
The Journal of biological chemistry279(31)32339-44(2004 Jul)
Lundequist A,Tchougounova E,Abrink M,Pejler G