Cacna1e cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Cacna1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa58134 XM_017598910.1
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa58135 XM_017598911.1
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa58136 XM_017598912.1
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa58137 XM_017598913.1
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa58138 XM_017598914.1
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa58139 XM_017598915.1
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa58140 XM_017598916.1
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ORa04303 NM_019294.2
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1984.50
$2835.00
ORa35498 XM_006250069.3
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa35498 XM_017598909.1
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa35498 XM_017598908.1
Latest version!
Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa58134
    Clone ID Related Accession (Same CDS sequence) XM_017598910.1
    Accession Version XM_017598910.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GCGGCAGGAT CCCGTATAAG GATATGTACA AATTAGTGCG GGTGATCTCG
    CCTCCTCTGG GCCTGGGAGA GAACTGCCCC TACAGGGTGG CTTGCAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGAGCCAGA GGTTAGTGAA
    TTAAAAAGTG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTCCGGACAC CCAGGAGCAT
    GCGGGATCTG GGAGGGCATC CTCTATGCCA CGTCTGACTA TGGATCCCCA GGTGGTAACA
    GACCCTAGCT CTATGCGACG TTCATTTTCT ACAATTCGGG ATAAGCGTTC AAATTCCTCC
    TGGTTGGAGG AGTTCTCCAT GGAACGTAGC AGTGAAAACA CCTACAAGTC ACGACGCAGA
    AGTTACCATT CCTCCCTGAG GCTGTCAGCC CACCGCCTGA ATTCTGACTC TGGTCACAAG
    TCTGACACTC ACCGCTCAGG AGGCAGGGAG AGAGGCCGAT CCAAAGAACG AAAGCATCTC
    CTCTCTCCGG ATGTCTCCCG CTGCAATTCT GAGGAGCGAG GAACCCAGGC TGACTGGGAG
    TCCCCAGAAC GTCGCCAGTC CAGGTCACCC AGTGAGGGAA GGTCACAAAC CCCCAACAGA
    CAGGGTACAG GTTCACTGAG TGAGAGCTCC ATTCCCTCTA TCTCCGACAC CAGCACCCCA
    AGACGAAGTC GTCGCCAGCT CCCACCTGTG CCACCAAAGC CTCGGCCCCT CCTCTCCTAC
    AGCTCCCTGA TGAGACACAC TGGCGGCATC TCTCCACCTC CTGATGGAAG TGAGGGTGGA
    TCCCCACTGG CCTCTCAAGC TCTGGAAAGC AACAGCGCTT GCCTAACCGA GTCTTCCAAC
    TCCTTGCACC CCCAGCAGGG CCAGCACCCT TCCCCACAGC ACTACATCTC TGAGCCCTAT
    CTGGCCCTCC ATGAAGACTC CCACGCCTCA GACTGTGGCG AGGAGGAGAC ACTCACCTTT
    GAGGCAGCCG TAGCTACTAG CTTGGGCCGG TCCAATACCA TTGGCTCTGC CCCACCCCTA
    CGGCACAGCT GGCAGATGCC TAATGGGCAC TATCGGCGGC GGAGGCGTGG GGGGCCTGGG
    CCTGGCATGA TGTGTGGAGC TGTCAGTGAC CTCCTGAGTG ACACGGAAGA AGACGATAAG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454399.1
    CDS562..7947
    Translation

    Target ORF information:

    RefSeq Version XM_017598910.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GCGGCAGGAT CCCGTATAAG GATATGTACA AATTAGTGCG GGTGATCTCG
    CCTCCTCTGG GCCTGGGAGA GAACTGCCCC TACAGGGTGG CTTGCAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGAGCCAGA GGTTAGTGAA
    TTAAAAAGTG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTCCGGACAC CCAGGAGCAT
    GCGGGATCTG GGAGGGCATC CTCTATGCCA CGTCTGACTA TGGATCCCCA GGTGGTAACA
    GACCCTAGCT CTATGCGACG TTCATTTTCT ACAATTCGGG ATAAGCGTTC AAATTCCTCC
    TGGTTGGAGG AGTTCTCCAT GGAACGTAGC AGTGAAAACA CCTACAAGTC ACGACGCAGA
    AGTTACCATT CCTCCCTGAG GCTGTCAGCC CACCGCCTGA ATTCTGACTC TGGTCACAAG
    TCTGACACTC ACCGCTCAGG AGGCAGGGAG AGAGGCCGAT CCAAAGAACG AAAGCATCTC
    CTCTCTCCGG ATGTCTCCCG CTGCAATTCT GAGGAGCGAG GAACCCAGGC TGACTGGGAG
    TCCCCAGAAC GTCGCCAGTC CAGGTCACCC AGTGAGGGAA GGTCACAAAC CCCCAACAGA
    CAGGGTACAG GTTCACTGAG TGAGAGCTCC ATTCCCTCTA TCTCCGACAC CAGCACCCCA
    AGACGAAGTC GTCGCCAGCT CCCACCTGTG CCACCAAAGC CTCGGCCCCT CCTCTCCTAC
    AGCTCCCTGA TGAGACACAC TGGCGGCATC TCTCCACCTC CTGATGGAAG TGAGGGTGGA
    TCCCCACTGG CCTCTCAAGC TCTGGAAAGC AACAGCGCTT GCCTAACCGA GTCTTCCAAC
    TCCTTGCACC CCCAGCAGGG CCAGCACCCT TCCCCACAGC ACTACATCTC TGAGCCCTAT
    CTGGCCCTCC ATGAAGACTC CCACGCCTCA GACTGTGGCG AGGAGGAGAC ACTCACCTTT
    GAGGCAGCCG TAGCTACTAG CTTGGGCCGG TCCAATACCA TTGGCTCTGC CCCACCCCTA
    CGGCACAGCT GGCAGATGCC TAATGGGCAC TATCGGCGGC GGAGGCGTGG GGGGCCTGGG
    CCTGGCATGA TGTGTGGAGC TGTCAGTGAC CTCCTGAGTG ACACGGAAGA AGACGATAAG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58135
    Clone ID Related Accession (Same CDS sequence) XM_017598911.1
    Accession Version XM_017598911.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGAG GAGACGCCGA
    CACCACATGT CTGTGTGGGA ACAGCGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCACCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC ACTCAATGCT CATCCTAGCC TCTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTGGG CTTGGGTCTT GAAAAATGTG AAGAGGAGCG TATCAGCCGT
    GGAGGTTCTC TCAAGGGGGA CATAGGTGGC CTGACTAGCG TCCTGGACAA CCAGAGGAGC
    CCTTTGTCCC TGGGCAAACG GGAGCCACCG TGGCTGCCCA GATCCTGTCA TGGAAACTGT
    GACCCAACCC AGCAGGAGAC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAG
    TCTGCCTCTG CCTCCAGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCATCGATG GGGAGAAGGA ACACGAGCCT CAGAGCAGCC ACAGGAGCAA GGAGCCAACC
    ATCCATGAAG AAGAGAGAAC TCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCAGGAC TGGTAGGAGC TCTTGATGAG GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGCGAG
    CAAGCCCTGC TTGCGGATGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCT
    GACCTCTCCT GCATGACAAC CAACATGGAC AAGGCTACCA CTGAGAGCAC CAGTGTCACA
    GTCGCCATCC CTGACATGGA CCCCTTGGTG GACTCAACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAA GCAGAGACCA AAGAGGAAGA GGAAGAGGTG
    GAAAAGAAGA AGCAGAAGAA GGAGAAGCGG GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAAGG CTTGCCACTA CATTGTGAAT
    CTACGCTACT TTGAGATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCTGAGGACC CTGTACTAAC TAACTCAGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTGATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCGTACTT CCGAGACCTC TGGAATATCC TGGACTTTGT GGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GCGGGATATC
    AAGACGATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGAC CACTGAAAAC CATCAAGCGC
    TTGCCCAAAC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTTATGTTC ATCTTTGCCG TCATTGCTGT TCAGTTATTC
    AAGGGAAAGT TCTTTTATTG CACCGACAGT TCCAAGGACA CAGAGAAGGA ATGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAGTG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCG GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCCCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTAGAAAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAAAACAGGC ACACCTTCCA GTACCGCGTG
    TGGCACTTCG TAGTCTCGCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACCGTTGTGC TGATGATGAA GTACTATTCT GCTCCTTGGA CCTATGAGCT CGCCCTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTAGAAT GTGTCCTAAA GGTCATCGCT
    TTCGGCTTCT TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT TACCGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAC AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGCG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTCCC CTATGTCTGC
    CTTCTGATTG CCATGCTTTT CTTCATCTAC GCTATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCATATCAAC CGGCACAACA ACTTTCGGAG TTTCTTCGGG
    TCTCTCATGC TGCTTTTCAG GAGCGCCACA GGGGAGGCTT GGCAGGAGAT TATGTTGTCA
    TGTCTGGGCG AGAAGGGCTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAACGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT TGTGGCTGTT ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCGTCCA TCCTGGGGCC TCACCACTTG GATGAATTTG TCCGTGTCTG GGCAGAGTAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGTTGGTC
    CTGATGAATA TGCCAGTGGC TGAGGACATG ACAGTCCATT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCAGAGC TACAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTAG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATTA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CCTCACTGCC TCAGGAGATC
    ATTTCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAGCAAT CGCTGGAGCC AGAGGTTAGT
    GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG
    CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA
    ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC
    TCCTGGTTGG AGGAGTTCTC CATGGAACGT AGCAGTGAAA ACACCTACAA GTCACGACGC
    AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCCCACCGCC TGAATTCTGA CTCTGGTCAC
    AAGTCTGACA CTCACCGCTC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAT
    CTCCTCTCTC CGGATGTCTC CCGCTGCAAT TCTGAGGAGC GAGGAACCCA GGCTGACTGG
    GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAGG GAAGGTCACA AACCCCCAAC
    AGACAGGGTA CAGGTTCACT GAGTGAGAGC TCCATTCCCT CTATCTCCGA CACCAGCACC
    CCAAGACGAA GTCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGGCC CCTCCTCTCC
    TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT
    GGATCCCCAC TGGCCTCTCA AGCTCTGGAA AGCAACAGCG CTTGCCTAAC CGAGTCTTCC
    AACTCCTTGC ACCCCCAGCA GGGCCAGCAC CCTTCCCCAC AGCACTACAT CTCTGAGCCC
    TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC
    TTTGAGGCAG CCGTAGCTAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCCCCACCC
    CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT
    GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT
    AAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454400.1
    CDS561..7889
    Translation

    Target ORF information:

    RefSeq Version XM_017598911.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598911.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGAG GAGACGCCGA
    CACCACATGT CTGTGTGGGA ACAGCGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCACCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC ACTCAATGCT CATCCTAGCC TCTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTGGG CTTGGGTCTT GAAAAATGTG AAGAGGAGCG TATCAGCCGT
    GGAGGTTCTC TCAAGGGGGA CATAGGTGGC CTGACTAGCG TCCTGGACAA CCAGAGGAGC
    CCTTTGTCCC TGGGCAAACG GGAGCCACCG TGGCTGCCCA GATCCTGTCA TGGAAACTGT
    GACCCAACCC AGCAGGAGAC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAG
    TCTGCCTCTG CCTCCAGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCATCGATG GGGAGAAGGA ACACGAGCCT CAGAGCAGCC ACAGGAGCAA GGAGCCAACC
    ATCCATGAAG AAGAGAGAAC TCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCAGGAC TGGTAGGAGC TCTTGATGAG GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGCGAG
    CAAGCCCTGC TTGCGGATGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCT
    GACCTCTCCT GCATGACAAC CAACATGGAC AAGGCTACCA CTGAGAGCAC CAGTGTCACA
    GTCGCCATCC CTGACATGGA CCCCTTGGTG GACTCAACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAA GCAGAGACCA AAGAGGAAGA GGAAGAGGTG
    GAAAAGAAGA AGCAGAAGAA GGAGAAGCGG GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAAGG CTTGCCACTA CATTGTGAAT
    CTACGCTACT TTGAGATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCTGAGGACC CTGTACTAAC TAACTCAGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTGATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCGTACTT CCGAGACCTC TGGAATATCC TGGACTTTGT GGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GCGGGATATC
    AAGACGATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGAC CACTGAAAAC CATCAAGCGC
    TTGCCCAAAC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTTATGTTC ATCTTTGCCG TCATTGCTGT TCAGTTATTC
    AAGGGAAAGT TCTTTTATTG CACCGACAGT TCCAAGGACA CAGAGAAGGA ATGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAGTG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCG GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCCCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTAGAAAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAAAACAGGC ACACCTTCCA GTACCGCGTG
    TGGCACTTCG TAGTCTCGCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACCGTTGTGC TGATGATGAA GTACTATTCT GCTCCTTGGA CCTATGAGCT CGCCCTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTAGAAT GTGTCCTAAA GGTCATCGCT
    TTCGGCTTCT TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT TACCGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAC AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGCG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTCCC CTATGTCTGC
    CTTCTGATTG CCATGCTTTT CTTCATCTAC GCTATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCATATCAAC CGGCACAACA ACTTTCGGAG TTTCTTCGGG
    TCTCTCATGC TGCTTTTCAG GAGCGCCACA GGGGAGGCTT GGCAGGAGAT TATGTTGTCA
    TGTCTGGGCG AGAAGGGCTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAACGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT TGTGGCTGTT ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCGTCCA TCCTGGGGCC TCACCACTTG GATGAATTTG TCCGTGTCTG GGCAGAGTAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGTTGGTC
    CTGATGAATA TGCCAGTGGC TGAGGACATG ACAGTCCATT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCAGAGC TACAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTAG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATTA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CCTCACTGCC TCAGGAGATC
    ATTTCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAGCAAT CGCTGGAGCC AGAGGTTAGT
    GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG
    CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA
    ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC
    TCCTGGTTGG AGGAGTTCTC CATGGAACGT AGCAGTGAAA ACACCTACAA GTCACGACGC
    AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCCCACCGCC TGAATTCTGA CTCTGGTCAC
    AAGTCTGACA CTCACCGCTC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAT
    CTCCTCTCTC CGGATGTCTC CCGCTGCAAT TCTGAGGAGC GAGGAACCCA GGCTGACTGG
    GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAGG GAAGGTCACA AACCCCCAAC
    AGACAGGGTA CAGGTTCACT GAGTGAGAGC TCCATTCCCT CTATCTCCGA CACCAGCACC
    CCAAGACGAA GTCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGGCC CCTCCTCTCC
    TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT
    GGATCCCCAC TGGCCTCTCA AGCTCTGGAA AGCAACAGCG CTTGCCTAAC CGAGTCTTCC
    AACTCCTTGC ACCCCCAGCA GGGCCAGCAC CCTTCCCCAC AGCACTACAT CTCTGAGCCC
    TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC
    TTTGAGGCAG CCGTAGCTAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCCCCACCC
    CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT
    GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT
    AAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58136
    Clone ID Related Accession (Same CDS sequence) XM_017598912.1
    Accession Version XM_017598912.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGGCAC ACCTCTTGCC
    AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT GGAGCCTCCT ATTTCCGGCA CAAGGAACGG
    CTTCTGCGTA TCTCTATCCG ACACATGGTC AAGTCGCAAG TGTTCTACTG GATCGTCCTG
    AGTGTTGTGG CTCTCAATAC TGCCTGTGTG GCCATCGTTC ATCACAACCA GCCACAGTGG
    CTCACTCACC TCCTCTACTA TGCAGAATTT TTGTTTCTGG GACTCTTCCT GTTGGAGATG
    TCCCTGAAGA TGTATGGCAT GGGGCCACGC CTTTATTTTC ACTCATCCTT CAACTGCTTT
    GATTTTGGGG TCACAGTGGG CAGCATCTTT GAGGTCGTTT GGGCAATCTT CCGACCTGGC
    ACATCTTTTG GAATCAGTGT CTTACGAGCT CTCCGGCTCC TAAGGATATT TAAAATAACC
    AAGTATTGGG CATCCCTCCG GAATCTGGTT GTTTCCTTGA TGAGCTCCAT GAAGTCTATC
    ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC ATTGTTGTCT TTGCTCTCCT GGGAATGCAG
    CTCTTTGGAG GCAGGTTTAA CTTTAATGAT GGGACTCCTT CGGCTAATTT TGATACCTTC
    CCTGCAGCTA TCATGACAGT GTTCCAGATC CTGACGGGGG AAGACTGGAA TGAAGTGATG
    TATAACGGCA TCCGCTCCCA GGGTGGGGTG AGCTCAGGCA TGTGGTCTGC CATCTACTTC
    ATTGTGCTCA CCTTGTTTGG AAACTACACA CTGCTGAATG TATTCTTGGC TATCGCTGTG
    GACAATCTCG CCAATGCCCA GGAACTGACC AAGGATGAGC AGGAGGAAGA AGAAGCCTTC
    AACCAGAAAC ATGCACTGCA GAAGGCCAAG GAGGTCAGCC CGATGTCTGC TCCCAACATG
    CCTTCCATTG AAAGAGACAG AAGGAGAAGA CACCACATGT CGATGTGGGA GCCGCGCAGC
    AGCCACCTGA GGGAGAGGAG ACGCCGACAC CACATGTCTG TGTGGGAACA GCGCACCAGC
    CAGCTGAGGA GGCACATGCA AATGTCTAGC CAAGAAGCCC TCAACAAAGA GGAGGCCCCA
    CCAATGAACC CCCTCAATCC ACTCAACCCA CTGAGTCCTC TCAACCCACT CAATGCTCAT
    CCTAGCCTCT ATCGGAGGCC CAGGCCCATT GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA
    AAATGTGAAG AGGAGCGTAT CAGCCGTGGA GGTTCTCTCA AGGGGGACAT AGGTGGCCTG
    ACTAGCGTCC TGGACAACCA GAGGAGCCCT TTGTCCCTGG GCAAACGGGA GCCACCGTGG
    CTGCCCAGAT CCTGTCATGG AAACTGTGAC CCAACCCAGC AGGAGACTGG GGGAGGAGAG
    ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG CACAGGCAGA GTCAGAGGCG AAGCCGGCAT
    CGCCGAGTCA GGACTGAGGG CAAGGAGTCT GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC
    CAGGAGCGGA GTCTGGATGA AGGGGTGTCC ATCGATGGGG AGAAGGAACA CGAGCCTCAG
    AGCAGCCACA GGAGCAAGGA GCCAACCATC CATGAAGAAG AGAGAACTCA GGACTTAAGG
    AGGACCAACA GTCTGATGGT CCCCAGGGGC TCAGGACTGG TAGGAGCTCT TGATGAGGCA
    GAAACACCTC TAGTCCAGCC CCAACCTGAG CTGGAAGTGG GGAAGGATGC AGCTCTGACA
    GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA GCCCTGCTTG CGGATGTACA GCTGGATGTG
    GGCCGGGGCA TTAGCCAGAG TGAACCTGAC CTCTCCTGCA TGACAACCAA CATGGACAAG
    GCTACCACTG AGAGCACCAG TGTCACAGTC GCCATCCCTG ACATGGACCC CTTGGTGGAC
    TCAACAGTGG TGAACATTAG CAACAAGACG GATGGAGAAG CCAGTCCCTT GAAGGAAGCA
    GAGACCAAAG AGGAAGAGGA AGAGGTGGAA AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG
    ACTGGAAAAG CCATGGTGCC CCATAGCTCT ATGTTCATCT TCAGCACCAC TAACCCGATC
    CGCAAGGCTT GCCACTACAT TGTGAATCTA CGCTACTTTG AGATGTGCAT TCTCTTGGTG
    ATTGCGGCCA GCAGCATTGC CCTGGCAGCT GAGGACCCTG TACTAACTAA CTCAGAGCGT
    AACAAAGTCC TGAGATATTT CGACTATGTT TTCACAGGAG TGTTTACCTT TGAGATGGTG
    ATAAAGATGA TAGACCAAGG CTTGATCCTG CAGGATGGAT CGTACTTCCG AGACCTCTGG
    AATATCCTGG ACTTTGTGGT AGTGGTTGGT GCATTGGTAG CATTTGCTCT GGCGAACGCT
    TTGGGAACCA ACAAGGGGCG GGATATCAAG ACGATCAAGT CTCTGAGGGT ACTTCGAGTT
    CTGCGACCAC TGAAAACCAT CAAGCGCTTG CCCAAACTGA AGGCTGTGTT TGACTGCGTG
    GTGACCTCCT TGAAGAATGT CTTCAACATA CTCATTGTCT ACAAGCTCTT TATGTTCATC
    TTTGCCGTCA TTGCTGTTCA GTTATTCAAG GGAAAGTTCT TTTATTGCAC CGACAGTTCC
    AAGGACACAG AGAAGGAATG CATAGGCAAC TATGTAGACC ACGAGAAAAA CAAGATGGAG
    GTGAAGGGCC GGGAGTGGAA GCGTCATGAA TTCCACTATG ACAACATCAT CTGGGCCCTG
    CTGACCCTCT TCACCGTCTC CACGGGGGAA GGATGGCCTC AGGTTCTGCA GCACTCGGTA
    GATGTGACAG AGGAGGACAG AGGCCCAAGC CGCAGCAACC GCATGGAGAT GTCCATCTTC
    TATGTGGTCT ACTTTGTGGT CTTCCCTTTC TTCTTTGTCA ATATCTTCGT GGCCCTCATT
    ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAGG AGTGTAGCCT AGAAAAGAAT
    GAGCGGGCAT GCATCGACTT CGCCATTAGT GCCAAACCTC TCACCCGCTA CATGCCACAA
    AACAGGCACA CCTTCCAGTA CCGCGTGTGG CACTTCGTAG TCTCGCCATC CTTTGAGTAT
    ACCATCATGG CCATGATTGC CTTGAACACC GTTGTGCTGA TGATGAAGTA CTATTCTGCT
    CCTTGGACCT ATGAGCTCGC CCTAAAGTAC CTGAACATCG CCTTCACTAT GGTGTTTTCC
    CTAGAATGTG TCCTAAAGGT CATCGCTTTC GGCTTCTTGA ACTATTTCCG AGATACCTGG
    AACATCTTTG ACTTCATTAC CGTGATCGGC AGTATCACAG AAATTATCCT GACAGACAGC
    AAGCTGGTGA ACACCAGTGG CTTCAATATG AGCTTTCTGA AGCTCTTCCG AGCTGCACGG
    CTCATAAAGC TTCTGCGACA GGGCTACACC ATCCGCATCT TACTGTGGAC CTTCGTGCAG
    TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT CTGATTGCCA TGCTTTTCTT CATCTACGCT
    ATCATTGGGA TGCAGGTGTT TGGAAACATA AAGTTAGATG AGGAGAGTCA TATCAACCGG
    CACAACAACT TTCGGAGTTT CTTCGGGTCT CTCATGCTGC TTTTCAGGAG CGCCACAGGG
    GAGGCTTGGC AGGAGATTAT GTTGTCATGT CTGGGCGAGA AGGGCTGTGA GCCTGACACC
    ACTGCACCCT CAGGGCAGAA CGAGAGTGAG CGCTGTGGCA CTGACCTGGC CTACGTTTAT
    TTTGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTTGT GGCTGTTATC
    ATGGACAACT TTGAGTACCT GACTCGAGAT TCGTCCATCC TGGGGCCTCA CCACTTGGAT
    GAATTTGTCC GTGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT
    GAGATGTATG AAATGCTGAC TCTCATGTCA CCACCGCTAG GCCTCGGCAA GAGATGTCCC
    TCCAAAGTGG CCTATAAGAG GTTGGTCCTG ATGAATATGC CAGTGGCTGA GGACATGACA
    GTCCATTTCA CCTCCACACT TATGGCTCTG ATCCGGACAG CTCTGGACAT TAAAATCGCC
    AAAGGTGGTG CAGACAGACA GCAGCTAGAC TCAGAGCTAC AAAAAGAGAC CCTGGCCATT
    TGGCCTCACC TGTCCCAAAA GATGTTGGAT CTGCTAGTGC CCATGCCCAA AGCCTCTGAC
    CTGACTGTGG GCAAGATCTA TGCAGCAATG ATGATTATGG ACTACTATAA ACAGAGTAAG
    GTAAAGAAAC AGAGACAGCA GCTGGAAGAA CAGAAAAATG CACCCATGTT CCAGCGCATG
    GAGCCCTCCT CACTGCCTCA GGAGATCATT TCTAATGCCA AAGCCCTGCC TTATCTCCAG
    CAGGACCCTG TTTCTGGCCT GAGTGGTCGG AGTGGATACC CTTCAATGAG TCCACTCTCC
    CCTCAAGAAA TATTCCAGTT GGCTTGTATG GACCCAGCGG ATGATGGACA ATTCCAGGAA
    CAGCAATCGC TGGAGCCAGA GGTTAGTGAA TTAAAAAGTG TGCAGTCCTC TAACCATGGC
    ATCTACCTTC CTCCGGACAC CCAGGAGCAT GCGGGATCTG GGAGGGCATC CTCTATGCCA
    CGTCTGACTA TGGATCCCCA GGTGGTAACA GACCCTAGCT CTATGCGACG TTCATTTTCT
    ACAATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AGTTCTCCAT GGAACGTAGC
    AGTGAAAACA CCTACAAGTC ACGACGCAGA AGTTACCATT CCTCCCTGAG GCTGTCAGCC
    CACCGCCTGA ATTCTGACTC TGGTCACAAG TCTGACACTC ACCGCTCAGG AGGCAGGGAG
    AGAGGCCGAT CCAAAGAACG AAAGCATCTC CTCTCTCCGG ATGTCTCCCG CTGCAATTCT
    GAGGAGCGAG GAACCCAGGC TGACTGGGAG TCCCCAGAAC GTCGCCAGTC CAGGTCACCC
    AGTGAGGGAA GGTCACAAAC CCCCAACAGA CAGGGTACAG GTTCACTGAG TGAGAGCTCC
    ATTCCCTCTA TCTCCGACAC CAGCACCCCA AGACGAAGTC GTCGCCAGCT CCCACCTGTG
    CCACCAAAGC CTCGGCCCCT CCTCTCCTAC AGCTCCCTGA TGAGACACAC TGGCGGCATC
    TCTCCACCTC CTGATGGAAG TGAGGGTGGA TCCCCACTGG CCTCTCAAGC TCTGGAAAGC
    AACAGCGCTT GCCTAACCGA GTCTTCCAAC TCCTTGCACC CCCAGCAGGG CCAGCACCCT
    TCCCCACAGC ACTACATCTC TGAGCCCTAT CTGGCCCTCC ATGAAGACTC CCACGCCTCA
    GACTGTGGCG AGGAGGAGAC ACTCACCTTT GAGGCAGCCG TAGCTACTAG CTTGGGCCGG
    TCCAATACCA TTGGCTCTGC CCCACCCCTA CGGCACAGCT GGCAGATGCC TAATGGGCAC
    TATCGGCGGC GGAGGCGTGG GGGGCCTGGG CCTGGCATGA TGTGTGGAGC TGTCAGTGAC
    CTCCTGAGTG ACACGGAAGA AGACGATAAG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454401.1
    CDS561..7856
    Translation

    Target ORF information:

    RefSeq Version XM_017598912.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGGCAC ACCTCTTGCC
    AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT GGAGCCTCCT ATTTCCGGCA CAAGGAACGG
    CTTCTGCGTA TCTCTATCCG ACACATGGTC AAGTCGCAAG TGTTCTACTG GATCGTCCTG
    AGTGTTGTGG CTCTCAATAC TGCCTGTGTG GCCATCGTTC ATCACAACCA GCCACAGTGG
    CTCACTCACC TCCTCTACTA TGCAGAATTT TTGTTTCTGG GACTCTTCCT GTTGGAGATG
    TCCCTGAAGA TGTATGGCAT GGGGCCACGC CTTTATTTTC ACTCATCCTT CAACTGCTTT
    GATTTTGGGG TCACAGTGGG CAGCATCTTT GAGGTCGTTT GGGCAATCTT CCGACCTGGC
    ACATCTTTTG GAATCAGTGT CTTACGAGCT CTCCGGCTCC TAAGGATATT TAAAATAACC
    AAGTATTGGG CATCCCTCCG GAATCTGGTT GTTTCCTTGA TGAGCTCCAT GAAGTCTATC
    ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC ATTGTTGTCT TTGCTCTCCT GGGAATGCAG
    CTCTTTGGAG GCAGGTTTAA CTTTAATGAT GGGACTCCTT CGGCTAATTT TGATACCTTC
    CCTGCAGCTA TCATGACAGT GTTCCAGATC CTGACGGGGG AAGACTGGAA TGAAGTGATG
    TATAACGGCA TCCGCTCCCA GGGTGGGGTG AGCTCAGGCA TGTGGTCTGC CATCTACTTC
    ATTGTGCTCA CCTTGTTTGG AAACTACACA CTGCTGAATG TATTCTTGGC TATCGCTGTG
    GACAATCTCG CCAATGCCCA GGAACTGACC AAGGATGAGC AGGAGGAAGA AGAAGCCTTC
    AACCAGAAAC ATGCACTGCA GAAGGCCAAG GAGGTCAGCC CGATGTCTGC TCCCAACATG
    CCTTCCATTG AAAGAGACAG AAGGAGAAGA CACCACATGT CGATGTGGGA GCCGCGCAGC
    AGCCACCTGA GGGAGAGGAG ACGCCGACAC CACATGTCTG TGTGGGAACA GCGCACCAGC
    CAGCTGAGGA GGCACATGCA AATGTCTAGC CAAGAAGCCC TCAACAAAGA GGAGGCCCCA
    CCAATGAACC CCCTCAATCC ACTCAACCCA CTGAGTCCTC TCAACCCACT CAATGCTCAT
    CCTAGCCTCT ATCGGAGGCC CAGGCCCATT GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA
    AAATGTGAAG AGGAGCGTAT CAGCCGTGGA GGTTCTCTCA AGGGGGACAT AGGTGGCCTG
    ACTAGCGTCC TGGACAACCA GAGGAGCCCT TTGTCCCTGG GCAAACGGGA GCCACCGTGG
    CTGCCCAGAT CCTGTCATGG AAACTGTGAC CCAACCCAGC AGGAGACTGG GGGAGGAGAG
    ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG CACAGGCAGA GTCAGAGGCG AAGCCGGCAT
    CGCCGAGTCA GGACTGAGGG CAAGGAGTCT GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC
    CAGGAGCGGA GTCTGGATGA AGGGGTGTCC ATCGATGGGG AGAAGGAACA CGAGCCTCAG
    AGCAGCCACA GGAGCAAGGA GCCAACCATC CATGAAGAAG AGAGAACTCA GGACTTAAGG
    AGGACCAACA GTCTGATGGT CCCCAGGGGC TCAGGACTGG TAGGAGCTCT TGATGAGGCA
    GAAACACCTC TAGTCCAGCC CCAACCTGAG CTGGAAGTGG GGAAGGATGC AGCTCTGACA
    GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA GCCCTGCTTG CGGATGTACA GCTGGATGTG
    GGCCGGGGCA TTAGCCAGAG TGAACCTGAC CTCTCCTGCA TGACAACCAA CATGGACAAG
    GCTACCACTG AGAGCACCAG TGTCACAGTC GCCATCCCTG ACATGGACCC CTTGGTGGAC
    TCAACAGTGG TGAACATTAG CAACAAGACG GATGGAGAAG CCAGTCCCTT GAAGGAAGCA
    GAGACCAAAG AGGAAGAGGA AGAGGTGGAA AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG
    ACTGGAAAAG CCATGGTGCC CCATAGCTCT ATGTTCATCT TCAGCACCAC TAACCCGATC
    CGCAAGGCTT GCCACTACAT TGTGAATCTA CGCTACTTTG AGATGTGCAT TCTCTTGGTG
    ATTGCGGCCA GCAGCATTGC CCTGGCAGCT GAGGACCCTG TACTAACTAA CTCAGAGCGT
    AACAAAGTCC TGAGATATTT CGACTATGTT TTCACAGGAG TGTTTACCTT TGAGATGGTG
    ATAAAGATGA TAGACCAAGG CTTGATCCTG CAGGATGGAT CGTACTTCCG AGACCTCTGG
    AATATCCTGG ACTTTGTGGT AGTGGTTGGT GCATTGGTAG CATTTGCTCT GGCGAACGCT
    TTGGGAACCA ACAAGGGGCG GGATATCAAG ACGATCAAGT CTCTGAGGGT ACTTCGAGTT
    CTGCGACCAC TGAAAACCAT CAAGCGCTTG CCCAAACTGA AGGCTGTGTT TGACTGCGTG
    GTGACCTCCT TGAAGAATGT CTTCAACATA CTCATTGTCT ACAAGCTCTT TATGTTCATC
    TTTGCCGTCA TTGCTGTTCA GTTATTCAAG GGAAAGTTCT TTTATTGCAC CGACAGTTCC
    AAGGACACAG AGAAGGAATG CATAGGCAAC TATGTAGACC ACGAGAAAAA CAAGATGGAG
    GTGAAGGGCC GGGAGTGGAA GCGTCATGAA TTCCACTATG ACAACATCAT CTGGGCCCTG
    CTGACCCTCT TCACCGTCTC CACGGGGGAA GGATGGCCTC AGGTTCTGCA GCACTCGGTA
    GATGTGACAG AGGAGGACAG AGGCCCAAGC CGCAGCAACC GCATGGAGAT GTCCATCTTC
    TATGTGGTCT ACTTTGTGGT CTTCCCTTTC TTCTTTGTCA ATATCTTCGT GGCCCTCATT
    ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAGG AGTGTAGCCT AGAAAAGAAT
    GAGCGGGCAT GCATCGACTT CGCCATTAGT GCCAAACCTC TCACCCGCTA CATGCCACAA
    AACAGGCACA CCTTCCAGTA CCGCGTGTGG CACTTCGTAG TCTCGCCATC CTTTGAGTAT
    ACCATCATGG CCATGATTGC CTTGAACACC GTTGTGCTGA TGATGAAGTA CTATTCTGCT
    CCTTGGACCT ATGAGCTCGC CCTAAAGTAC CTGAACATCG CCTTCACTAT GGTGTTTTCC
    CTAGAATGTG TCCTAAAGGT CATCGCTTTC GGCTTCTTGA ACTATTTCCG AGATACCTGG
    AACATCTTTG ACTTCATTAC CGTGATCGGC AGTATCACAG AAATTATCCT GACAGACAGC
    AAGCTGGTGA ACACCAGTGG CTTCAATATG AGCTTTCTGA AGCTCTTCCG AGCTGCACGG
    CTCATAAAGC TTCTGCGACA GGGCTACACC ATCCGCATCT TACTGTGGAC CTTCGTGCAG
    TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT CTGATTGCCA TGCTTTTCTT CATCTACGCT
    ATCATTGGGA TGCAGGTGTT TGGAAACATA AAGTTAGATG AGGAGAGTCA TATCAACCGG
    CACAACAACT TTCGGAGTTT CTTCGGGTCT CTCATGCTGC TTTTCAGGAG CGCCACAGGG
    GAGGCTTGGC AGGAGATTAT GTTGTCATGT CTGGGCGAGA AGGGCTGTGA GCCTGACACC
    ACTGCACCCT CAGGGCAGAA CGAGAGTGAG CGCTGTGGCA CTGACCTGGC CTACGTTTAT
    TTTGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTTGT GGCTGTTATC
    ATGGACAACT TTGAGTACCT GACTCGAGAT TCGTCCATCC TGGGGCCTCA CCACTTGGAT
    GAATTTGTCC GTGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT
    GAGATGTATG AAATGCTGAC TCTCATGTCA CCACCGCTAG GCCTCGGCAA GAGATGTCCC
    TCCAAAGTGG CCTATAAGAG GTTGGTCCTG ATGAATATGC CAGTGGCTGA GGACATGACA
    GTCCATTTCA CCTCCACACT TATGGCTCTG ATCCGGACAG CTCTGGACAT TAAAATCGCC
    AAAGGTGGTG CAGACAGACA GCAGCTAGAC TCAGAGCTAC AAAAAGAGAC CCTGGCCATT
    TGGCCTCACC TGTCCCAAAA GATGTTGGAT CTGCTAGTGC CCATGCCCAA AGCCTCTGAC
    CTGACTGTGG GCAAGATCTA TGCAGCAATG ATGATTATGG ACTACTATAA ACAGAGTAAG
    GTAAAGAAAC AGAGACAGCA GCTGGAAGAA CAGAAAAATG CACCCATGTT CCAGCGCATG
    GAGCCCTCCT CACTGCCTCA GGAGATCATT TCTAATGCCA AAGCCCTGCC TTATCTCCAG
    CAGGACCCTG TTTCTGGCCT GAGTGGTCGG AGTGGATACC CTTCAATGAG TCCACTCTCC
    CCTCAAGAAA TATTCCAGTT GGCTTGTATG GACCCAGCGG ATGATGGACA ATTCCAGGAA
    CAGCAATCGC TGGAGCCAGA GGTTAGTGAA TTAAAAAGTG TGCAGTCCTC TAACCATGGC
    ATCTACCTTC CTCCGGACAC CCAGGAGCAT GCGGGATCTG GGAGGGCATC CTCTATGCCA
    CGTCTGACTA TGGATCCCCA GGTGGTAACA GACCCTAGCT CTATGCGACG TTCATTTTCT
    ACAATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AGTTCTCCAT GGAACGTAGC
    AGTGAAAACA CCTACAAGTC ACGACGCAGA AGTTACCATT CCTCCCTGAG GCTGTCAGCC
    CACCGCCTGA ATTCTGACTC TGGTCACAAG TCTGACACTC ACCGCTCAGG AGGCAGGGAG
    AGAGGCCGAT CCAAAGAACG AAAGCATCTC CTCTCTCCGG ATGTCTCCCG CTGCAATTCT
    GAGGAGCGAG GAACCCAGGC TGACTGGGAG TCCCCAGAAC GTCGCCAGTC CAGGTCACCC
    AGTGAGGGAA GGTCACAAAC CCCCAACAGA CAGGGTACAG GTTCACTGAG TGAGAGCTCC
    ATTCCCTCTA TCTCCGACAC CAGCACCCCA AGACGAAGTC GTCGCCAGCT CCCACCTGTG
    CCACCAAAGC CTCGGCCCCT CCTCTCCTAC AGCTCCCTGA TGAGACACAC TGGCGGCATC
    TCTCCACCTC CTGATGGAAG TGAGGGTGGA TCCCCACTGG CCTCTCAAGC TCTGGAAAGC
    AACAGCGCTT GCCTAACCGA GTCTTCCAAC TCCTTGCACC CCCAGCAGGG CCAGCACCCT
    TCCCCACAGC ACTACATCTC TGAGCCCTAT CTGGCCCTCC ATGAAGACTC CCACGCCTCA
    GACTGTGGCG AGGAGGAGAC ACTCACCTTT GAGGCAGCCG TAGCTACTAG CTTGGGCCGG
    TCCAATACCA TTGGCTCTGC CCCACCCCTA CGGCACAGCT GGCAGATGCC TAATGGGCAC
    TATCGGCGGC GGAGGCGTGG GGGGCCTGGG CCTGGCATGA TGTGTGGAGC TGTCAGTGAC
    CTCCTGAGTG ACACGGAAGA AGACGATAAG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58137
    Clone ID Related Accession (Same CDS sequence) XM_017598913.1
    Accession Version XM_017598913.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GTGGCCGCAT CCATTACACT GAGATGTATG AAATGCTGAC TCTCATGTCA
    CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CCTATAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGTGGTAAC AGACCCTAGC
    TCTATGCGAC GTTCATTTTC TACAATTCGG GATAAGCGTT CAAATTCCTC CTGGTTGGAG
    GAGTTCTCCA TGGAACGTAG CAGTGAAAAC ACCTACAAGT CACGACGCAG AAGTTACCAT
    TCCTCCCTGA GGCTGTCAGC CCACCGCCTG AATTCTGACT CTGGTCACAA GTCTGACACT
    CACCGCTCAG GAGGCAGGGA GAGAGGCCGA TCCAAAGAAC GAAAGCATCT CCTCTCTCCG
    GATGTCTCCC GCTGCAATTC TGAGGAGCGA GGAACCCAGG CTGACTGGGA GTCCCCAGAA
    CGTCGCCAGT CCAGGTCACC CAGTGAGGGA AGGTCACAAA CCCCCAACAG ACAGGGTACA
    GGTTCACTGA GTGAGAGCTC CATTCCCTCT ATCTCCGACA CCAGCACCCC AAGACGAAGT
    CGTCGCCAGC TCCCACCTGT GCCACCAAAG CCTCGGCCCC TCCTCTCCTA CAGCTCCCTG
    ATGAGACACA CTGGCGGCAT CTCTCCACCT CCTGATGGAA GTGAGGGTGG ATCCCCACTG
    GCCTCTCAAG CTCTGGAAAG CAACAGCGCT TGCCTAACCG AGTCTTCCAA CTCCTTGCAC
    CCCCAGCAGG GCCAGCACCC TTCCCCACAG CACTACATCT CTGAGCCCTA TCTGGCCCTC
    CATGAAGACT CCCACGCCTC AGACTGTGGC GAGGAGGAGA CACTCACCTT TGAGGCAGCC
    GTAGCTACTA GCTTGGGCCG GTCCAATACC ATTGGCTCTG CCCCACCCCT ACGGCACAGC
    TGGCAGATGC CTAATGGGCA CTATCGGCGG CGGAGGCGTG GGGGGCCTGG GCCTGGCATG
    ATGTGTGGAG CTGTCAGTGA CCTCCTGAGT GACACGGAAG AAGACGATAA GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454402.1
    CDS560..7816
    Translation

    Target ORF information:

    RefSeq Version XM_017598913.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598913.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GTGGCCGCAT CCATTACACT GAGATGTATG AAATGCTGAC TCTCATGTCA
    CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CCTATAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGTGGTAAC AGACCCTAGC
    TCTATGCGAC GTTCATTTTC TACAATTCGG GATAAGCGTT CAAATTCCTC CTGGTTGGAG
    GAGTTCTCCA TGGAACGTAG CAGTGAAAAC ACCTACAAGT CACGACGCAG AAGTTACCAT
    TCCTCCCTGA GGCTGTCAGC CCACCGCCTG AATTCTGACT CTGGTCACAA GTCTGACACT
    CACCGCTCAG GAGGCAGGGA GAGAGGCCGA TCCAAAGAAC GAAAGCATCT CCTCTCTCCG
    GATGTCTCCC GCTGCAATTC TGAGGAGCGA GGAACCCAGG CTGACTGGGA GTCCCCAGAA
    CGTCGCCAGT CCAGGTCACC CAGTGAGGGA AGGTCACAAA CCCCCAACAG ACAGGGTACA
    GGTTCACTGA GTGAGAGCTC CATTCCCTCT ATCTCCGACA CCAGCACCCC AAGACGAAGT
    CGTCGCCAGC TCCCACCTGT GCCACCAAAG CCTCGGCCCC TCCTCTCCTA CAGCTCCCTG
    ATGAGACACA CTGGCGGCAT CTCTCCACCT CCTGATGGAA GTGAGGGTGG ATCCCCACTG
    GCCTCTCAAG CTCTGGAAAG CAACAGCGCT TGCCTAACCG AGTCTTCCAA CTCCTTGCAC
    CCCCAGCAGG GCCAGCACCC TTCCCCACAG CACTACATCT CTGAGCCCTA TCTGGCCCTC
    CATGAAGACT CCCACGCCTC AGACTGTGGC GAGGAGGAGA CACTCACCTT TGAGGCAGCC
    GTAGCTACTA GCTTGGGCCG GTCCAATACC ATTGGCTCTG CCCCACCCCT ACGGCACAGC
    TGGCAGATGC CTAATGGGCA CTATCGGCGG CGGAGGCGTG GGGGGCCTGG GCCTGGCATG
    ATGTGTGGAG CTGTCAGTGA CCTCCTGAGT GACACGGAAG AAGACGATAA GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58138
    Clone ID Related Accession (Same CDS sequence) XM_017598914.1
    Accession Version XM_017598914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGAG GAGACGCCGA
    CACCACATGT CTGTGTGGGA ACAGCGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCACCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC ACTCAATGCT CATCCTAGCC TCTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTGGG CTTGGGTCTT GAAAAATGTG AAGAGGAGCG TATCAGCCGT
    GGAGGTTCTC TCAAGGGGGA CATAGGTGGC CTGACTAGCG TCCTGGACAA CCAGAGGAGC
    CCTTTGTCCC TGGGCAAACG GGAGCCACCG TGGCTGCCCA GATCCTGTCA TGGAAACTGT
    GACCCAACCC AGCAGGAGAC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAG
    TCTGCCTCTG CCTCCAGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCATCGATG GGGAGAAGGA ACACGAGCCT CAGAGCAGCC ACAGGAGCAA GGAGCCAACC
    ATCCATGAAG AAGAGAGAAC TCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCAGGAC TGGTAGGAGC TCTTGATGAG GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGCGAG
    CAAGCCCTGC TTGCGGATGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCT
    GACCTCTCCT GCATGACAAC CAACATGGAC AAGGCTACCA CTGAGAGCAC CAGTGTCACA
    GTCGCCATCC CTGACATGGA CCCCTTGGTG GACTCAACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAA GCAGAGACCA AAGAGGAAGA GGAAGAGGTG
    GAAAAGAAGA AGCAGAAGAA GGAGAAGCGG GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAAGG CTTGCCACTA CATTGTGAAT
    CTACGCTACT TTGAGATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCTGAGGACC CTGTACTAAC TAACTCAGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTGATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCGTACTT CCGAGACCTC TGGAATATCC TGGACTTTGT GGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GCGGGATATC
    AAGACGATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGAC CACTGAAAAC CATCAAGCGC
    TTGCCCAAAC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTTATGTTC ATCTTTGCCG TCATTGCTGT TCAGTTATTC
    AAGGGAAAGT TCTTTTATTG CACCGACAGT TCCAAGGACA CAGAGAAGGA ATGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAGTG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCG GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCCCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTAGAAAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAAAACAGGC ACACCTTCCA GTACCGCGTG
    TGGCACTTCG TAGTCTCGCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACCGTTGTGC TGATGATGAA GTACTATTCT GCTCCTTGGA CCTATGAGCT CGCCCTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTAGAAT GTGTCCTAAA GGTCATCGCT
    TTCGGCTTCT TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT TACCGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAC AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGCG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTCCC CTATGTCTGC
    CTTCTGATTG CCATGCTTTT CTTCATCTAC GCTATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCATATCAAC CGGCACAACA ACTTTCGGAG TTTCTTCGGG
    TCTCTCATGC TGCTTTTCAG GAGCGCCACA GGGGAGGCTT GGCAGGAGAT TATGTTGTCA
    TGTCTGGGCG AGAAGGGCTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAACGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT TGTGGCTGTT ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCGTCCA TCCTGGGGCC TCACCACTTG GATGAATTTG TCCGTGTCTG GGCAGAGTAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGTTGGTC
    CTGATGAATA TGCCAGTGGC TGAGGACATG ACAGTCCATT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCAGAGC TACAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTAG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATTA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CCTCACTGCC TCAGGAGATC
    ATTTCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAGCAAT CGCTGGTGGT AACAGACCCT
    AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG
    GAGGAGTTCT CCATGGAACG TAGCAGTGAA AACACCTACA AGTCACGACG CAGAAGTTAC
    CATTCCTCCC TGAGGCTGTC AGCCCACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC
    ACTCACCGCT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA TCTCCTCTCT
    CCGGATGTCT CCCGCTGCAA TTCTGAGGAG CGAGGAACCC AGGCTGACTG GGAGTCCCCA
    GAACGTCGCC AGTCCAGGTC ACCCAGTGAG GGAAGGTCAC AAACCCCCAA CAGACAGGGT
    ACAGGTTCAC TGAGTGAGAG CTCCATTCCC TCTATCTCCG ACACCAGCAC CCCAAGACGA
    AGTCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGGC CCCTCCTCTC CTACAGCTCC
    CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA
    CTGGCCTCTC AAGCTCTGGA AAGCAACAGC GCTTGCCTAA CCGAGTCTTC CAACTCCTTG
    CACCCCCAGC AGGGCCAGCA CCCTTCCCCA CAGCACTACA TCTCTGAGCC CTATCTGGCC
    CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA
    GCCGTAGCTA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCCCCACC CCTACGGCAC
    AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC
    ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454403.1
    CDS560..7759
    Translation

    Target ORF information:

    RefSeq Version XM_017598914.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGAG GAGACGCCGA
    CACCACATGT CTGTGTGGGA ACAGCGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCACCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC ACTCAATGCT CATCCTAGCC TCTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTGGG CTTGGGTCTT GAAAAATGTG AAGAGGAGCG TATCAGCCGT
    GGAGGTTCTC TCAAGGGGGA CATAGGTGGC CTGACTAGCG TCCTGGACAA CCAGAGGAGC
    CCTTTGTCCC TGGGCAAACG GGAGCCACCG TGGCTGCCCA GATCCTGTCA TGGAAACTGT
    GACCCAACCC AGCAGGAGAC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAG
    TCTGCCTCTG CCTCCAGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCATCGATG GGGAGAAGGA ACACGAGCCT CAGAGCAGCC ACAGGAGCAA GGAGCCAACC
    ATCCATGAAG AAGAGAGAAC TCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCAGGAC TGGTAGGAGC TCTTGATGAG GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGCGAG
    CAAGCCCTGC TTGCGGATGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCT
    GACCTCTCCT GCATGACAAC CAACATGGAC AAGGCTACCA CTGAGAGCAC CAGTGTCACA
    GTCGCCATCC CTGACATGGA CCCCTTGGTG GACTCAACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAA GCAGAGACCA AAGAGGAAGA GGAAGAGGTG
    GAAAAGAAGA AGCAGAAGAA GGAGAAGCGG GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAAGG CTTGCCACTA CATTGTGAAT
    CTACGCTACT TTGAGATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCTGAGGACC CTGTACTAAC TAACTCAGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTGATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCGTACTT CCGAGACCTC TGGAATATCC TGGACTTTGT GGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GCGGGATATC
    AAGACGATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGAC CACTGAAAAC CATCAAGCGC
    TTGCCCAAAC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTTATGTTC ATCTTTGCCG TCATTGCTGT TCAGTTATTC
    AAGGGAAAGT TCTTTTATTG CACCGACAGT TCCAAGGACA CAGAGAAGGA ATGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAGTG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCG GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCCCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTAGAAAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAAAACAGGC ACACCTTCCA GTACCGCGTG
    TGGCACTTCG TAGTCTCGCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACCGTTGTGC TGATGATGAA GTACTATTCT GCTCCTTGGA CCTATGAGCT CGCCCTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTAGAAT GTGTCCTAAA GGTCATCGCT
    TTCGGCTTCT TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT TACCGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAC AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGCG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTCCC CTATGTCTGC
    CTTCTGATTG CCATGCTTTT CTTCATCTAC GCTATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCATATCAAC CGGCACAACA ACTTTCGGAG TTTCTTCGGG
    TCTCTCATGC TGCTTTTCAG GAGCGCCACA GGGGAGGCTT GGCAGGAGAT TATGTTGTCA
    TGTCTGGGCG AGAAGGGCTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAACGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT TGTGGCTGTT ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCGTCCA TCCTGGGGCC TCACCACTTG GATGAATTTG TCCGTGTCTG GGCAGAGTAT
    GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG
    TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGTTGGTC
    CTGATGAATA TGCCAGTGGC TGAGGACATG ACAGTCCATT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCAGAGC TACAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTAG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATTA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CCTCACTGCC TCAGGAGATC
    ATTTCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAGCAAT CGCTGGTGGT AACAGACCCT
    AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG
    GAGGAGTTCT CCATGGAACG TAGCAGTGAA AACACCTACA AGTCACGACG CAGAAGTTAC
    CATTCCTCCC TGAGGCTGTC AGCCCACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC
    ACTCACCGCT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA TCTCCTCTCT
    CCGGATGTCT CCCGCTGCAA TTCTGAGGAG CGAGGAACCC AGGCTGACTG GGAGTCCCCA
    GAACGTCGCC AGTCCAGGTC ACCCAGTGAG GGAAGGTCAC AAACCCCCAA CAGACAGGGT
    ACAGGTTCAC TGAGTGAGAG CTCCATTCCC TCTATCTCCG ACACCAGCAC CCCAAGACGA
    AGTCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGGC CCCTCCTCTC CTACAGCTCC
    CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA
    CTGGCCTCTC AAGCTCTGGA AAGCAACAGC GCTTGCCTAA CCGAGTCTTC CAACTCCTTG
    CACCCCCAGC AGGGCCAGCA CCCTTCCCCA CAGCACTACA TCTCTGAGCC CTATCTGGCC
    CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA
    GCCGTAGCTA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCCCCACC CCTACGGCAC
    AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC
    ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58139
    Clone ID Related Accession (Same CDS sequence) XM_017598915.1
    Accession Version XM_017598915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGAG GAGACGCCGA
    CACCACATGT CTGTGTGGGA ACAGCGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCACCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC ACTCAATGCT CATCCTAGCC TCTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTGGG CTTGGGTCTT GAAAAATGTG AAGAGGAGCG TATCAGCCGT
    GGAGGTTCTC TCAAGGGGGA CATAGGTGGC CTGACTAGCG TCCTGGACAA CCAGAGGAGC
    CCTTTGTCCC TGGGCAAACG GGAGCCACCG TGGCTGCCCA GATCCTGTCA TGGAAACTGT
    GACCCAACCC AGCAGGAGAC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAG
    TCTGCCTCTG CCTCCAGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCATCGATG GGGAGAAGGA ACACGAGCCT CAGAGCAGCC ACAGGAGCAA GGAGCCAACC
    ATCCATGAAG AAGAGAGAAC TCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCAGGAC TGGTAGGAGC TCTTGATGAG GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGCGAG
    CAAGCCCTGC TTGCGGATGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCT
    GACCTCTCCT GCATGACAAC CAACATGGAC AAGGCTACCA CTGAGAGCAC CAGTGTCACA
    GTCGCCATCC CTGACATGGA CCCCTTGGTG GACTCAACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAA GCAGAGACCA AAGAGGAAGA GGAAGAGGTG
    GAAAAGAAGA AGCAGAAGAA GGAGAAGCGG GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAAGG CTTGCCACTA CATTGTGAAT
    CTACGCTACT TTGAGATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCTGAGGACC CTGTACTAAC TAACTCAGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTGATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCGTACTT CCGAGACCTC TGGAATATCC TGGACTTTGT GGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GCGGGATATC
    AAGACGATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGAC CACTGAAAAC CATCAAGCGC
    TTGCCCAAAC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTTATGTTC ATCTTTGCCG TCATTGCTGT TCAGTTATTC
    AAGGGAAAGT TCTTTTATTG CACCGACAGT TCCAAGGACA CAGAGAAGGA ATGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAGTG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCG GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCCCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTAGAAAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAAAACAGGC ACACCTTCCA GTACCGCGTG
    TGGCACTTCG TAGTCTCGCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACCGTTGTGC TGATGATGAA GTACTATTCT GCTCCTTGGA CCTATGAGCT CGCCCTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTAGAAT GTGTCCTAAA GGTCATCGCT
    TTCGGCTTCT TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT TACCGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAC AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGCG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTCCC CTATGTCTGC
    CTTCTGATTG CCATGCTTTT CTTCATCTAC GCTATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCATATCAAC CGGCACAACA ACTTTCGGAG TTTCTTCGGG
    TCTCTCATGC TGCTTTTCAG GAGCGCCACA GGGGAGGCTT GGCAGGAGAT TATGTTGTCA
    TGTCTGGGCG AGAAGGGCTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAACGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT TGTGGCTGTT ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCGTCCA TCCTGGGGCC TCACCACTTG GATGAATTTG TCCGTGTCTG GGCAGAGTAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGTTGGTC
    CTGATGAATA TGCCAGTGGC TGAGGACATG ACAGTCCATT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCAGAGC TACAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTAG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATTA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CCTCACTGCC TCAGGAGATC
    ATTTCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAGCAAT CGCTGGTGGT AACAGACCCT
    AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG
    GAGGAGTTCT CCATGGAACG TAGCAGTGAA AACACCTACA AGTCACGACG CAGAAGTTAC
    CATTCCTCCC TGAGGCTGTC AGCCCACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC
    ACTCACCGCT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA TCTCCTCTCT
    CCGGATGTCT CCCGCTGCAA TTCTGAGGAG CGAGGAACCC AGGCTGACTG GGAGTCCCCA
    GAACGTCGCC AGTCCAGGTC ACCCAGTGAG GGAAGGTCAC AAACCCCCAA CAGACAGGGT
    ACAGGTTCAC TGAGTGAGAG CTCCATTCCC TCTATCTCCG ACACCAGCAC CCCAAGACGA
    AGTCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGGC CCCTCCTCTC CTACAGCTCC
    CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA
    CTGGCCTCTC AAGCTCTGGA AAGCAACAGC GCTTGCCTAA CCGAGTCTTC CAACTCCTTG
    CACCCCCAGC AGGGCCAGCA CCCTTCCCCA CAGCACTACA TCTCTGAGCC CTATCTGGCC
    CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA
    GCCGTAGCTA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCCCCACC CCTACGGCAC
    AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC
    ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454404.1
    CDS561..7760
    Translation

    Target ORF information:

    RefSeq Version XM_017598915.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGAG GAGACGCCGA
    CACCACATGT CTGTGTGGGA ACAGCGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT
    AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCACCAATGA ACCCCCTCAA TCCACTCAAC
    CCACTGAGTC CTCTCAACCC ACTCAATGCT CATCCTAGCC TCTATCGGAG GCCCAGGCCC
    ATTGAGGGCC TAGCCCTGGG CTTGGGTCTT GAAAAATGTG AAGAGGAGCG TATCAGCCGT
    GGAGGTTCTC TCAAGGGGGA CATAGGTGGC CTGACTAGCG TCCTGGACAA CCAGAGGAGC
    CCTTTGTCCC TGGGCAAACG GGAGCCACCG TGGCTGCCCA GATCCTGTCA TGGAAACTGT
    GACCCAACCC AGCAGGAGAC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC
    AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAG
    TCTGCCTCTG CCTCCAGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG
    TCCATCGATG GGGAGAAGGA ACACGAGCCT CAGAGCAGCC ACAGGAGCAA GGAGCCAACC
    ATCCATGAAG AAGAGAGAAC TCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG
    GGCTCAGGAC TGGTAGGAGC TCTTGATGAG GCAGAAACAC CTCTAGTCCA GCCCCAACCT
    GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGCGAG
    CAAGCCCTGC TTGCGGATGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCT
    GACCTCTCCT GCATGACAAC CAACATGGAC AAGGCTACCA CTGAGAGCAC CAGTGTCACA
    GTCGCCATCC CTGACATGGA CCCCTTGGTG GACTCAACAG TGGTGAACAT TAGCAACAAG
    ACGGATGGAG AAGCCAGTCC CTTGAAGGAA GCAGAGACCA AAGAGGAAGA GGAAGAGGTG
    GAAAAGAAGA AGCAGAAGAA GGAGAAGCGG GAGACTGGAA AAGCCATGGT GCCCCATAGC
    TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAAGG CTTGCCACTA CATTGTGAAT
    CTACGCTACT TTGAGATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA
    GCTGAGGACC CTGTACTAAC TAACTCAGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT
    GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTGATAAAGA TGATAGACCA AGGCTTGATC
    CTGCAGGATG GATCGTACTT CCGAGACCTC TGGAATATCC TGGACTTTGT GGTAGTGGTT
    GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GCGGGATATC
    AAGACGATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGAC CACTGAAAAC CATCAAGCGC
    TTGCCCAAAC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC
    ATACTCATTG TCTACAAGCT CTTTATGTTC ATCTTTGCCG TCATTGCTGT TCAGTTATTC
    AAGGGAAAGT TCTTTTATTG CACCGACAGT TCCAAGGACA CAGAGAAGGA ATGCATAGGC
    AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAGTG GAAGCGTCAT
    GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTGACCC TCTTCACCGT CTCCACGGGG
    GAAGGATGGC CTCAGGTTCT GCAGCACTCG GTAGATGTGA CAGAGGAGGA CAGAGGCCCA
    AGCCGCAGCA ACCGCATGGA GATGTCCATC TTCTATGTGG TCTACTTTGT GGTCTTCCCT
    TTCTTCTTTG TCAATATCTT CGTGGCCCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC
    AAGATGATGG AGGAGTGTAG CCTAGAAAAG AATGAGCGGG CATGCATCGA CTTCGCCATT
    AGTGCCAAAC CTCTCACCCG CTACATGCCA CAAAACAGGC ACACCTTCCA GTACCGCGTG
    TGGCACTTCG TAGTCTCGCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC
    ACCGTTGTGC TGATGATGAA GTACTATTCT GCTCCTTGGA CCTATGAGCT CGCCCTAAAG
    TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTAGAAT GTGTCCTAAA GGTCATCGCT
    TTCGGCTTCT TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT TACCGTGATC
    GGCAGTATCA CAGAAATTAT CCTGACAGAC AGCAAGCTGG TGAACACCAG TGGCTTCAAT
    ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGCG ACAGGGCTAC
    ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTCCC CTATGTCTGC
    CTTCTGATTG CCATGCTTTT CTTCATCTAC GCTATCATTG GGATGCAGGT GTTTGGAAAC
    ATAAAGTTAG ATGAGGAGAG TCATATCAAC CGGCACAACA ACTTTCGGAG TTTCTTCGGG
    TCTCTCATGC TGCTTTTCAG GAGCGCCACA GGGGAGGCTT GGCAGGAGAT TATGTTGTCA
    TGTCTGGGCG AGAAGGGCTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAACGAGAGT
    GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC
    TTCCTGATGC TCAACCTGTT TGTGGCTGTT ATCATGGACA ACTTTGAGTA CCTGACTCGA
    GATTCGTCCA TCCTGGGGCC TCACCACTTG GATGAATTTG TCCGTGTCTG GGCAGAGTAT
    GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC
    TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGTTGGTC
    CTGATGAATA TGCCAGTGGC TGAGGACATG ACAGTCCATT TCACCTCCAC ACTTATGGCT
    CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA
    GACTCAGAGC TACAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG
    GATCTGCTAG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA
    ATGATGATTA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA
    GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CCTCACTGCC TCAGGAGATC
    ATTTCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT
    CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT
    ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAGCAAT CGCTGGTGGT AACAGACCCT
    AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG
    GAGGAGTTCT CCATGGAACG TAGCAGTGAA AACACCTACA AGTCACGACG CAGAAGTTAC
    CATTCCTCCC TGAGGCTGTC AGCCCACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC
    ACTCACCGCT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA TCTCCTCTCT
    CCGGATGTCT CCCGCTGCAA TTCTGAGGAG CGAGGAACCC AGGCTGACTG GGAGTCCCCA
    GAACGTCGCC AGTCCAGGTC ACCCAGTGAG GGAAGGTCAC AAACCCCCAA CAGACAGGGT
    ACAGGTTCAC TGAGTGAGAG CTCCATTCCC TCTATCTCCG ACACCAGCAC CCCAAGACGA
    AGTCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGGC CCCTCCTCTC CTACAGCTCC
    CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA
    CTGGCCTCTC AAGCTCTGGA AAGCAACAGC GCTTGCCTAA CCGAGTCTTC CAACTCCTTG
    CACCCCCAGC AGGGCCAGCA CCCTTCCCCA CAGCACTACA TCTCTGAGCC CTATCTGGCC
    CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA
    GCCGTAGCTA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCCCCACC CCTACGGCAC
    AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC
    ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58140
    Clone ID Related Accession (Same CDS sequence) XM_017598916.1
    Accession Version XM_017598916.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGACCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTACCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATATGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCCCGAA GACTGGAGAA GACAGAACCA TATTTTATTG GAATCTTCTG CTTTGAAGCT
    GGGATCAAAA TCGTGGCTCT AGGATTCATC TTCCATAAGG GTTCGTACCT CCGTAACGGC
    TGGAATGTCA TGGACTTCAT TGTGGTCCTC AGTGGCATCC TGGCCACTGC AGGAACCCAT
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCCTTAAAG
    CTCGTGTCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CCATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATTC TCATGTTTGC CATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGATTTG ATCCTCCTCA CCCGTGTGGT GTACAGGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCTGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CCCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTTGT CCTGGGCGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC ATTCATGAAG CTCCGGCGTC AGCAACAGAT TGAGCGCGAG
    TTGAATGGCT ACCGCGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGAGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG TATCTCTATC CGACACATGG TCAAGTCGCA AGTGTTCTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTCAAT ACTGCCTGTG TGGCCATCGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAAT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTTTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTCGT TTGGGCAATC
    TTCCGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTTTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTC CTCTTCCTCT TCATTGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCGGCTAAT
    TTTGATACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACGGG GGAAGACTGG
    AATGAAGTGA TGTATAACGG CATCCGCTCC CAGGGTGGGG TGAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CACTGCTGAA TGTATTCTTG
    GCTATCGCTG TGGACAATCT CGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCGCGCA GCAGCCACCT GAGGGAGAGG AGACGCCGAC ACCACATGTC TGTGTGGGAA
    CAGCGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CACCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCA
    CTCAATGCTC ATCCTAGCCT CTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTGGGC
    TTGGGTCTTG AAAAATGTGA AGAGGAGCGT ATCAGCCGTG GAGGTTCTCT CAAGGGGGAC
    ATAGGTGGCC TGACTAGCGT CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG
    GAGCCACCGT GGCTGCCCAG ATCCTGTCAT GGAAACTGTG ACCCAACCCA GCAGGAGACT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGAGT CTGCCTCTGC CTCCAGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCATCGATGG GGAGAAGGAA
    CACGAGCCTC AGAGCAGCCA CAGGAGCAAG GAGCCAACCA TCCATGAAGA AGAGAGAACT
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCAGGACT GGTAGGAGCT
    CTTGATGAGG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGCGAGC AAGCCCTGCT TGCGGATGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCTG ACCTCTCCTG CATGACAACC
    AACATGGACA AGGCTACCAC TGAGAGCACC AGTGTCACAG TCGCCATCCC TGACATGGAC
    CCCTTGGTGG ACTCAACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAAG CAGAGACCAA AGAGGAAGAG GAAGAGGTGG AAAAGAAGAA GCAGAAGAAG
    GAGAAGCGGG AGACTGGAAA AGCCATGGTG CCCCATAGCT CTATGTTCAT CTTCAGCACC
    ACTAACCCGA TCCGCAAGGC TTGCCACTAC ATTGTGAATC TACGCTACTT TGAGATGTGC
    ATTCTCTTGG TGATTGCGGC CAGCAGCATT GCCCTGGCAG CTGAGGACCC TGTACTAACT
    AACTCAGAGC GTAACAAAGT CCTGAGATAT TTCGACTATG TTTTCACAGG AGTGTTTACC
    TTTGAGATGG TGATAAAGAT GATAGACCAA GGCTTGATCC TGCAGGATGG ATCGTACTTC
    CGAGACCTCT GGAATATCCT GGACTTTGTG GTAGTGGTTG GTGCATTGGT AGCATTTGCT
    CTGGCGAACG CTTTGGGAAC CAACAAGGGG CGGGATATCA AGACGATCAA GTCTCTGAGG
    GTACTTCGAG TTCTGCGACC ACTGAAAACC ATCAAGCGCT TGCCCAAACT GAAGGCTGTG
    TTTGACTGCG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTTATGTTCA TCTTTGCCGT CATTGCTGTT CAGTTATTCA AGGGAAAGTT CTTTTATTGC
    ACCGACAGTT CCAAGGACAC AGAGAAGGAA TGCATAGGCA ACTATGTAGA CCACGAGAAA
    AACAAGATGG AGGTGAAGGG CCGGGAGTGG AAGCGTCATG AATTCCACTA TGACAACATC
    ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAGGTTCTG
    CAGCACTCGG TAGATGTGAC AGAGGAGGAC AGAGGCCCAA GCCGCAGCAA CCGCATGGAG
    ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTC
    GTGGCCCTCA TTATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA GGAGTGTAGC
    CTAGAAAAGA ATGAGCGGGC ATGCATCGAC TTCGCCATTA GTGCCAAACC TCTCACCCGC
    TACATGCCAC AAAACAGGCA CACCTTCCAG TACCGCGTGT GGCACTTCGT AGTCTCGCCA
    TCCTTTGAGT ATACCATCAT GGCCATGATT GCCTTGAACA CCGTTGTGCT GATGATGAAG
    TACTATTCTG CTCCTTGGAC CTATGAGCTC GCCCTAAAGT ACCTGAACAT CGCCTTCACT
    ATGGTGTTTT CCCTAGAATG TGTCCTAAAG GTCATCGCTT TCGGCTTCTT GAACTATTTC
    CGAGATACCT GGAACATCTT TGACTTCATT ACCGTGATCG GCAGTATCAC AGAAATTATC
    CTGACAGACA GCAAGCTGGT GAACACCAGT GGCTTCAATA TGAGCTTTCT GAAGCTCTTC
    CGAGCTGCAC GGCTCATAAA GCTTCTGCGA CAGGGCTACA CCATCCGCAT CTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCTCTCCCC TATGTCTGCC TTCTGATTGC CATGCTTTTC
    TTCATCTACG CTATCATTGG GATGCAGGTG TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CATATCAACC GGCACAACAA CTTTCGGAGT TTCTTCGGGT CTCTCATGCT GCTTTTCAGG
    AGCGCCACAG GGGAGGCTTG GCAGGAGATT ATGTTGTCAT GTCTGGGCGA GAAGGGCTGT
    GAGCCTGACA CCACTGCACC CTCAGGGCAG AACGAGAGTG AGCGCTGTGG CACTGACCTG
    GCCTACGTTT ATTTTGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTT
    GTGGCTGTTA TCATGGACAA CTTTGAGTAC CTGACTCGAG ATTCGTCCAT CCTGGGGCCT
    CACCACTTGG ATGAATTTGT CCGTGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCACCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAAGT GGCCTATAAG AGGTTGGTCC TGATGAATAT GCCAGTGGCT
    GAGGACATGA CAGTCCATTT CACCTCCACA CTTATGGCTC TGATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGTGG TGCAGACAGA CAGCAGCTAG ACTCAGAGCT ACAAAAAGAG
    ACCCTGGCCA TTTGGCCTCA CCTGTCCCAA AAGATGTTGG ATCTGCTAGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAGATC TATGCAGCAA TGATGATTAT GGACTACTAT
    AAACAGAGTA AGGTAAAGAA ACAGAGACAG CAGCTGGAAG AACAGAAAAA TGCACCCATG
    TTCCAGCGCA TGGAGCCCTC CTCACTGCCT CAGGAGATCA TTTCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC TGTTTCTGGC CTGAGTGGTC GGAGTGGATA CCCTTCAATG
    AGTCCACTCT CCCCTCAAGA AATATTCCAG TTGGCTTGTA TGGACCCAGC GGATGATGGA
    CAATTCCAGG AACAGCAATC GCTGGAGCCA GAGGTTAGTG AATTAAAAAG TGTGCAGTCC
    TCTAACCATG GCATCTACCT TCCTCCGGAC ACCCAGGAGC ATGCGGGATC TGGGAGGGCA
    TCCTCTATGC CACGTCTGAC TATGGATCCC CAGGTGGTAA CAGACCCTAG CTCTATGCGA
    CGTTCATTTT CTACAATTCG GGATAAGCGT TCAAATTCCT CCTGGTTGGA GGAGTTCTCC
    ATGGAACGTA GCAGTGAAAA CACCTACAAG TCACGACGCA GAAGTTACCA TTCCTCCCTG
    AGGCTGTCAG CCCACCGCCT GAATTCTGAC TCTGGTCACA AGTCTGACAC TCACCGCTCA
    GGAGGCAGGG AGAGAGGCCG ATCCAAAGAA CGAAAGCATC TCCTCTCTCC GGATGTCTCC
    CGCTGCAATT CTGAGGAGCG AGGAACCCAG GCTGACTGGG AGTCCCCAGA ACGTCGCCAG
    TCCAGGTCAC CCAGTGAGGG AAGGTCACAA ACCCCCAACA GACAGGGTAC AGGTTCACTG
    AGTGAGAGCT CCATTCCCTC TATCTCCGAC ACCAGCACCC CAAGACGAAG TCGTCGCCAG
    CTCCCACCTG TGCCACCAAA GCCTCGGCCC CTCCTCTCCT ACAGCTCCCT GATGAGACAC
    ACTGGCGGCA TCTCTCCACC TCCTGATGGA AGTGAGGGTG GATCCCCACT GGCCTCTCAA
    GCTCTGGAAA GCAACAGCGC TTGCCTAACC GAGTCTTCCA ACTCCTTGCA CCCCCAGCAG
    GGCCAGCACC CTTCCCCACA GCACTACATC TCTGAGCCCT ATCTGGCCCT CCATGAAGAC
    TCCCACGCCT CAGACTGTGG CGAGGAGGAG ACACTCACCT TTGAGGCAGC CGTAGCTACT
    AGCTTGGGCC GGTCCAATAC CATTGGCTCT GCCCCACCCC TACGGCACAG CTGGCAGATG
    CCTAATGGGC ACTATCGGCG GCGGAGGCGT GGGGGGCCTG GGCCTGGCAT GATGTGTGGA
    GCTGTCAGTG ACCTCCTGAG TGACACGGAA GAAGACGATA AGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454405.1
    CDS743..7690
    Translation

    Target ORF information:

    RefSeq Version XM_017598916.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGACCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTACCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG
    CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG
    AAATATGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC
    ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA
    ATGTCCCGAA GACTGGAGAA GACAGAACCA TATTTTATTG GAATCTTCTG CTTTGAAGCT
    GGGATCAAAA TCGTGGCTCT AGGATTCATC TTCCATAAGG GTTCGTACCT CCGTAACGGC
    TGGAATGTCA TGGACTTCAT TGTGGTCCTC AGTGGCATCC TGGCCACTGC AGGAACCCAT
    TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCCTTAAAG
    CTCGTGTCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CCATCATGAA GGCCATGGTG
    CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATTC TCATGTTTGC CATCATTGGC
    TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA
    GAGGGATTTG ATCCTCCTCA CCCGTGTGGT GTACAGGGCT GCCCGGCTGG TTATGAATGT
    AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCTGTG
    CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT
    GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CCCTCATCAT CATCGGATCC
    TTCTTTGTTC TCAACCTTGT CCTGGGCGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG
    AGAGTGGAGA ACCGAAGAGC ATTCATGAAG CTCCGGCGTC AGCAACAGAT TGAGCGCGAG
    TTGAATGGCT ACCGCGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT
    AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG
    ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC
    ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGAGCCTC CTATTTCCGG
    CACAAGGAAC GGCTTCTGCG TATCTCTATC CGACACATGG TCAAGTCGCA AGTGTTCTAC
    TGGATCGTCC TGAGTGTTGT GGCTCTCAAT ACTGCCTGTG TGGCCATCGT TCATCACAAC
    CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAAT TTTTGTTTCT GGGACTCTTC
    CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTTTATTT TCACTCATCC
    TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTCGT TTGGGCAATC
    TTCCGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA
    TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTTTCCTT GATGAGCTCC
    ATGAAGTCTA TCATCAGCTT ACTCTTCCTC CTCTTCCTCT TCATTGTTGT CTTTGCTCTC
    CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCGGCTAAT
    TTTGATACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACGGG GGAAGACTGG
    AATGAAGTGA TGTATAACGG CATCCGCTCC CAGGGTGGGG TGAGCTCAGG CATGTGGTCT
    GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CACTGCTGAA TGTATTCTTG
    GCTATCGCTG TGGACAATCT CGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA
    GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT
    GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG
    GAGCCGCGCA GCAGCCACCT GAGGGAGAGG AGACGCCGAC ACCACATGTC TGTGTGGGAA
    CAGCGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CACCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCA
    CTCAATGCTC ATCCTAGCCT CTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTGGGC
    TTGGGTCTTG AAAAATGTGA AGAGGAGCGT ATCAGCCGTG GAGGTTCTCT CAAGGGGGAC
    ATAGGTGGCC TGACTAGCGT CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG
    GAGCCACCGT GGCTGCCCAG ATCCTGTCAT GGAAACTGTG ACCCAACCCA GCAGGAGACT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGAGT CTGCCTCTGC CTCCAGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCATCGATGG GGAGAAGGAA
    CACGAGCCTC AGAGCAGCCA CAGGAGCAAG GAGCCAACCA TCCATGAAGA AGAGAGAACT
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCAGGACT GGTAGGAGCT
    CTTGATGAGG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGCGAGC AAGCCCTGCT TGCGGATGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCTG ACCTCTCCTG CATGACAACC
    AACATGGACA AGGCTACCAC TGAGAGCACC AGTGTCACAG TCGCCATCCC TGACATGGAC
    CCCTTGGTGG ACTCAACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAAG CAGAGACCAA AGAGGAAGAG GAAGAGGTGG AAAAGAAGAA GCAGAAGAAG
    GAGAAGCGGG AGACTGGAAA AGCCATGGTG CCCCATAGCT CTATGTTCAT CTTCAGCACC
    ACTAACCCGA TCCGCAAGGC TTGCCACTAC ATTGTGAATC TACGCTACTT TGAGATGTGC
    ATTCTCTTGG TGATTGCGGC CAGCAGCATT GCCCTGGCAG CTGAGGACCC TGTACTAACT
    AACTCAGAGC GTAACAAAGT CCTGAGATAT TTCGACTATG TTTTCACAGG AGTGTTTACC
    TTTGAGATGG TGATAAAGAT GATAGACCAA GGCTTGATCC TGCAGGATGG ATCGTACTTC
    CGAGACCTCT GGAATATCCT GGACTTTGTG GTAGTGGTTG GTGCATTGGT AGCATTTGCT
    CTGGCGAACG CTTTGGGAAC CAACAAGGGG CGGGATATCA AGACGATCAA GTCTCTGAGG
    GTACTTCGAG TTCTGCGACC ACTGAAAACC ATCAAGCGCT TGCCCAAACT GAAGGCTGTG
    TTTGACTGCG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTTATGTTCA TCTTTGCCGT CATTGCTGTT CAGTTATTCA AGGGAAAGTT CTTTTATTGC
    ACCGACAGTT CCAAGGACAC AGAGAAGGAA TGCATAGGCA ACTATGTAGA CCACGAGAAA
    AACAAGATGG AGGTGAAGGG CCGGGAGTGG AAGCGTCATG AATTCCACTA TGACAACATC
    ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAGGTTCTG
    CAGCACTCGG TAGATGTGAC AGAGGAGGAC AGAGGCCCAA GCCGCAGCAA CCGCATGGAG
    ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTC
    GTGGCCCTCA TTATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA GGAGTGTAGC
    CTAGAAAAGA ATGAGCGGGC ATGCATCGAC TTCGCCATTA GTGCCAAACC TCTCACCCGC
    TACATGCCAC AAAACAGGCA CACCTTCCAG TACCGCGTGT GGCACTTCGT AGTCTCGCCA
    TCCTTTGAGT ATACCATCAT GGCCATGATT GCCTTGAACA CCGTTGTGCT GATGATGAAG
    TACTATTCTG CTCCTTGGAC CTATGAGCTC GCCCTAAAGT ACCTGAACAT CGCCTTCACT
    ATGGTGTTTT CCCTAGAATG TGTCCTAAAG GTCATCGCTT TCGGCTTCTT GAACTATTTC
    CGAGATACCT GGAACATCTT TGACTTCATT ACCGTGATCG GCAGTATCAC AGAAATTATC
    CTGACAGACA GCAAGCTGGT GAACACCAGT GGCTTCAATA TGAGCTTTCT GAAGCTCTTC
    CGAGCTGCAC GGCTCATAAA GCTTCTGCGA CAGGGCTACA CCATCCGCAT CTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCTCTCCCC TATGTCTGCC TTCTGATTGC CATGCTTTTC
    TTCATCTACG CTATCATTGG GATGCAGGTG TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CATATCAACC GGCACAACAA CTTTCGGAGT TTCTTCGGGT CTCTCATGCT GCTTTTCAGG
    AGCGCCACAG GGGAGGCTTG GCAGGAGATT ATGTTGTCAT GTCTGGGCGA GAAGGGCTGT
    GAGCCTGACA CCACTGCACC CTCAGGGCAG AACGAGAGTG AGCGCTGTGG CACTGACCTG
    GCCTACGTTT ATTTTGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTT
    GTGGCTGTTA TCATGGACAA CTTTGAGTAC CTGACTCGAG ATTCGTCCAT CCTGGGGCCT
    CACCACTTGG ATGAATTTGT CCGTGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCACCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAAGT GGCCTATAAG AGGTTGGTCC TGATGAATAT GCCAGTGGCT
    GAGGACATGA CAGTCCATTT CACCTCCACA CTTATGGCTC TGATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGTGG TGCAGACAGA CAGCAGCTAG ACTCAGAGCT ACAAAAAGAG
    ACCCTGGCCA TTTGGCCTCA CCTGTCCCAA AAGATGTTGG ATCTGCTAGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAGATC TATGCAGCAA TGATGATTAT GGACTACTAT
    AAACAGAGTA AGGTAAAGAA ACAGAGACAG CAGCTGGAAG AACAGAAAAA TGCACCCATG
    TTCCAGCGCA TGGAGCCCTC CTCACTGCCT CAGGAGATCA TTTCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC TGTTTCTGGC CTGAGTGGTC GGAGTGGATA CCCTTCAATG
    AGTCCACTCT CCCCTCAAGA AATATTCCAG TTGGCTTGTA TGGACCCAGC GGATGATGGA
    CAATTCCAGG AACAGCAATC GCTGGAGCCA GAGGTTAGTG AATTAAAAAG TGTGCAGTCC
    TCTAACCATG GCATCTACCT TCCTCCGGAC ACCCAGGAGC ATGCGGGATC TGGGAGGGCA
    TCCTCTATGC CACGTCTGAC TATGGATCCC CAGGTGGTAA CAGACCCTAG CTCTATGCGA
    CGTTCATTTT CTACAATTCG GGATAAGCGT TCAAATTCCT CCTGGTTGGA GGAGTTCTCC
    ATGGAACGTA GCAGTGAAAA CACCTACAAG TCACGACGCA GAAGTTACCA TTCCTCCCTG
    AGGCTGTCAG CCCACCGCCT GAATTCTGAC TCTGGTCACA AGTCTGACAC TCACCGCTCA
    GGAGGCAGGG AGAGAGGCCG ATCCAAAGAA CGAAAGCATC TCCTCTCTCC GGATGTCTCC
    CGCTGCAATT CTGAGGAGCG AGGAACCCAG GCTGACTGGG AGTCCCCAGA ACGTCGCCAG
    TCCAGGTCAC CCAGTGAGGG AAGGTCACAA ACCCCCAACA GACAGGGTAC AGGTTCACTG
    AGTGAGAGCT CCATTCCCTC TATCTCCGAC ACCAGCACCC CAAGACGAAG TCGTCGCCAG
    CTCCCACCTG TGCCACCAAA GCCTCGGCCC CTCCTCTCCT ACAGCTCCCT GATGAGACAC
    ACTGGCGGCA TCTCTCCACC TCCTGATGGA AGTGAGGGTG GATCCCCACT GGCCTCTCAA
    GCTCTGGAAA GCAACAGCGC TTGCCTAACC GAGTCTTCCA ACTCCTTGCA CCCCCAGCAG
    GGCCAGCACC CTTCCCCACA GCACTACATC TCTGAGCCCT ATCTGGCCCT CCATGAAGAC
    TCCCACGCCT CAGACTGTGG CGAGGAGGAG ACACTCACCT TTGAGGCAGC CGTAGCTACT
    AGCTTGGGCC GGTCCAATAC CATTGGCTCT GCCCCACCCC TACGGCACAG CTGGCAGATG
    CCTAATGGGC ACTATCGGCG GCGGAGGCGT GGGGGGCCTG GGCCTGGCAT GATGTGTGGA
    GCTGTCAGTG ACCTCCTGAG TGACACGGAA GAAGACGATA AGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa04303
    Clone ID Related Accession (Same CDS sequence) NM_019294.2
    Accession Version NM_019294.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY029412.1. On Apr 20, 2007 this sequence version replaced NM_019294.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY029412.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGACCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTACCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGACTATG GCTTTGTACA ACCCCATACC AGTCCGGCAG
    AACTGTTTCA CGGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AACTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCTTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGAAGAC TGGAGAAGAC AGAACCATAT TTTATTGGAA TCTTCTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCTCTAGG ATTCATCTTC CATAAGGGTT CGTACCTCCG TAACGGCTGG
    AATGTCATGG ACTTCATTGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCATTTC
    AACACCCACG TGGACCTGCG GACCCTCCGG GCTGTGCGTG TCCTGAGGCC CTTAAAGCTC
    GTGTCAGGAA TACCTAGCCT GCAGATCGTG CTGAAGTCCA TCATGAAGGC CATGGTGCCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATTCTCA TGTTTGCCAT CATTGGCTTG
    GAGTTCTACA GTGGCAAGTT GCATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAG
    GGATTTGATC CTCCTCACCC GTGTGGTGTA CAGGGCTGCC CGGCTGGTTA TGAATGTAAG
    GACTGGATCG GCCCCAATGA CGGGATCACC CAGTTTGACA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AATGCATCAC TATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA CTGGCTGTAC TTCATCCCCC TCATCATCAT CGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGCGTGCTT TCCGGGGAAT TTGCCAAAGA AAGAGAGAGA
    GTGGAGAACC GAAGAGCATT CATGAAGCTC CGGCGTCAGC AACAGATTGA GCGCGAGTTG
    AATGGCTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAATAAA
    AACTCGGGAA CATCAGCCTT GGAAGTGCTT CGAAGGGCAA CCATCAAAAG GAGCCGGACG
    GAGGCCATGA CCCGAGACTC CAGCGATGAG CACTGCGTTG ATATCTCTTC AGTGGGCACA
    CCTCTTGCCA GAGCCAGTAT CAAGAGCACA AAAGTAGACG GAGCCTCCTA TTTCCGGCAC
    AAGGAACGGC TTCTGCGTAT CTCTATCCGA CACATGGTCA AGTCGCAAGT GTTCTACTGG
    ATCGTCCTGA GTGTTGTGGC TCTCAATACT GCCTGTGTGG CCATCGTTCA TCACAACCAG
    CCACAGTGGC TCACTCACCT CCTCTACTAT GCAGAATTTT TGTTTCTGGG ACTCTTCCTG
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCACGCC TTTATTTTCA CTCATCCTTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGCATCTTTG AGGTCGTTTG GGCAATCTTC
    CGACCTGGCA CATCTTTTGG AATCAGTGTC TTACGAGCTC TCCGGCTCCT AAGGATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATCTGGTTG TTTCCTTGAT GAGCTCCATG
    AAGTCTATCA TCAGCTTACT CTTCCTCCTC TTCCTCTTCA TTGTTGTCTT TGCTCTCCTG
    GGAATGCAGC TCTTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCTAATTTT
    GATACCTTCC CTGCAGCTAT CATGACAGTG TTCCAGATCC TGACGGGGGA AGACTGGAAT
    GAAGTGCTGT ATAACGGCAT CCGCTCCCAG GGTGGGGTGA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGA AACTACACAC TGCTGAATGT ATTCTTGGCT
    ATCGCTGTGG ACAATCTCGC CAATGCCCAG GAACTGACCA AGGATGAGCA GGAGGAAGAA
    GAAGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCT
    CCCAACATGC CTTCCATTGA GAGGGAGAGG AGACGCCGAC ACCACATGTC TGTGTGGGAA
    CAGCGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CACCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCA
    CTCAATGCTC ATCCTAGCCT CTATCGGAGG CCCAGGCCCA TTGAGGGCCT ACCCCTGGGC
    TTGGGTCTTG AAAAATGTGA AGAGGAGCGT ATCAGCCGTG GAGGTTCTCT CAAGGGGGAC
    ATAGGTGGCC TGACTAGCGT CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG
    GAGCCACCGT GGCTGCCCAG ATCCTGTCAT GGAAACTGTG ACCCAACCCA GCAGGAGACT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGAGT CTGCCTCTGC CTCCAGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCATCGATGG GGAGAAGGAA
    CACGAGCCTC AGAGCAGCCA CAGGAGCAAG GAGCCAACCA TCCATGAAGA AGAGAGAACT
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCAGGACT GGTAGGAGCT
    CTTGATGAGG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGCGAGC AAGCCCTGCT TGCGGATGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCTG ACCTCTCCTG CATGACAACC
    AACATGGACA AGGCTACCAC TGAGAGCACC AGTGTCACAG TCGCCATCCC TGACGTGGAC
    CCCTTGGTGG ACTCAACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAAG CAGAGACCAA AGAGGAAGAG GAAGAGGTGG AAAAGAAGAA GCAGAAGAAG
    GAGAAGCGGG AGACTGGAAA AGCCATGGTG CCCCATAGCT CTATGTTCAT CTTCAGCACC
    ACTAACCCGA TCCGCAAGGC TTGCCACTAC ATTGTGAATC TACGCTACTT TGAGATGTGC
    ATTCTCTTGG TGATTGCGGC CAGCAGCATT GCCCTGGCAG CTGAGGACCC TGTACTAACT
    AACTCAGAGC GTAACAAAGT CCTGAGATAT TTCGACTATG TTTTCACAGG AGTGTTTACC
    TTTGAGATGG TGATAAAGAT GATAGACCAA GGCTTGATCC TGCAGGATGG ATCGTACTTC
    CGAGACCTCT GGAATATCCT GGACTTTGTG GTAGTGGTTG GTGCATTGGT AGCATTTGCT
    CTGGCGAACG CTTTGGGAAC CAACAAGGGG CGGGATATCA AGACGATCAA GTCTCTGAGG
    GTACTTCGAG TTCTGCGACC ACTGAAAACC ATCAAGCGCT TGCCCAAACT GAAGGCTGTG
    TTTGACTGCG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTTATGTTCA TCTTTGCCGT CATTGCTGTT CAGTTATTCA AGGGAAAGTT CTTTTATTGC
    ACCGACAGTT CCAAGGACAC AGAGAAGGAA TGCATAGGCA ACTATGTAGA CCACGAGAAA
    AACAAGATGG AGGTGAAGGG CCGGGAGTGG AAGCGTCATG AATTCCACTA TGACAACATC
    ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAGGTTCTG
    CAGCACTCGG TAGATGTGAC AGAGGAGGAC AGAGGCCCAA GCCGCAGCAA CCGCATGGAG
    ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTC
    GTGGCCCTCA TTATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA GGAGTGTAGC
    CTAGAAAAGA ATGAGCGGGC ATGCATCGAC TTCGCCATTA GTGCCAAACC TCTCACCCGC
    TACATGCCAC AGAACAGGCA CACCTTCCAG TACCGCGTGT GGCACTTCGT AGTCTCGCCA
    TCCTTTGAGT ATACCATCAT GGCCATGATT GCCTTGAACA CCGTTGTGCT GATGATGAAG
    TACTATTCTG CTCCTTGGAC CTATGAGCTC GCCCTAAAGT ACCTGAACAT CGCCTTCACT
    ATGGTGTTTT CCCTAGAATG TGTCCTAAAG GTCATCGCTT TCGGCTTCTT GAACTATTTC
    CGAGATACCT GGAACATCTT TGACTTCATT ACCGTGATCG GCAGTATCAC AGAAATTATC
    CTGACAGACA GCAAGCTGGT GAACACCAGT GGCTTCAATA TGAGCTTTCT GGAGCTCTTC
    CGAGCTGCAC GGCTCATAAA GCTTCTGCGA CAGGGCTACA CCATCCGCAT CTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCTCTCCCC TATGTCTGCC TTCTGATTGC CATGCTTTTC
    TTCATCTACG CTATCATTGG GATGCAGGTG TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CATATCAACC GGCACAACAA CTTTCGGAGT TTCTTCGGGT CTCTCATGCT GCTTTTCAGG
    AGCGCCACAG GGGAGGCTTG GCAGGAGATT ATGTTGTCAT GTCTGGGCGA GAAGGGCTGT
    GAGCCTGACA CCACTGCACC CTCAGGGCAG AACGAGAGTG AGCGCTGTGG CACTGACCTG
    GCCTACGTTT ATTTTGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTT
    GTGGCTGTTA TCATGGACAA CTTTGAGTAC CTGACTCGAG ATTCGTCCAT CCTGGGGCCT
    CACCACTTGG ATGAATTTGT CCGTGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCACCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAAGT GGCCTATAAG AGGTTGGTCC TGATGAATAT GCCAGTGGCT
    GAGGACATGA CAGTCCATTT CACCTCCACA CTTATGGCTC TGATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGTGG TGCAGACAGA CAGCAGCTAG ACTCAGAGCT ACAAAAAGAG
    ACCCTGGCCA TTTGGCCTCA CCTGTCCCAA AAGATGTTGG ATCTGCTAGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAGATC TATGCAGCAA TGATGATTAT GGACTACTAT
    AAACAGAGTA AGGTAAAGAA ACAGAGACAG CAGCTGGAAG AACAGAAAAA TGCACCCATG
    TTCCAGCGCA TGGAGCCCTC CTCACTGCCT CAGGAGATCA TTTCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC TGTTTCTGGC CTGAGTGGTC GGAGTGGATA CCCTTCAATG
    AGTCCACTCT CCCCTCAAGA AATATTCCAG TTGGCTTGTA TGGACCCAGC GGATGATGGA
    CAATTCCAGG AACAGCAATC GCTGGAGCCA GAGGTTAGTG AATTAAAAAG TGTGCAGTCC
    TCTAACCATG GCATCTACCT TCCTCCGGAC ACCCAGGAGC ATGCGGGATC TGGGAGGGCA
    TCCTCTATGC CACGTCTGAC TATGGATCCC CAGGTGGTAA CAGACCCTAG CTCTATGCGA
    CGTTCATTTT CTACAATTCG GGATAAGCGT TCAAATTCCT CCTGGTTGGA GGAGTTCTCC
    ATGGAACGTA GCAGTGAAAA CACCTACAAG TCACGACGCA GAAGTTACCA TTCCTCCCTG
    AGGCTGTCAG CCCACCGCCT GAATTCTGAC TCTGGTCACA AGTCTGACAC TCACCGCTCA
    GGAGGCAGGG AGAGAGGCCG ATCCAAAGAA CGAAAGCATC TCCTCTCTCC GGATGTCTCC
    CGCTGCAATT CTGAGGAGCG AGGAACCCAG GCTGACTGGG AGTCCCCAGA ACGTCGCCAG
    TCCAGGTCAC CCAGTGAGGG AAGGTCACAA ACCCCCAACA GACAGGGTAC AGGTTCACTG
    AGTGAGAGCT CCATTCCCTC TATCTCCGAC ACCAGCACCC CAAGACGAAG TCGTCGCCAG
    CTCCCACCTG TGCCACCAAA GCCTCGGCCC CTCCTCTCCT ACAGCTCCCT GATGAGACAC
    ACTGGCGGCA TCTCTCCACC TCCTGATGGA AGTGAGGGTG GATCCCCACT GGCCTCTCAA
    GCTCTGGAAA GCAACAGCGC TTGCCTAACC GAGTCTTCCA ACTCCTTGCA CCCCCAGCAG
    GGCCAGCACC CTTCCCCACA GCACTACATC TCTGAGCCCT ATCTGGCCCT CCATGAAGAC
    TCCCACGCCT CAGACTGTGG CGAGGAGGAG ACACTCACCT TTGAGGCAGC TGTAGCTACT
    AGCTTGGGCC GGTCCAATAC CATTGGCTCT GCCCCACCCC TACGGCACAG CTGGCAGATG
    CCTAATGGGC ACTATCGGCG GCGGAGGCTG GGGGGCCTGG GCCTGGCCAT GATGTGTGGA
    GCTGTCAGTG ACCTCCTGAG TGACACGGAA GAAGACGATA AGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_062167.2
    CDS8..6895
    Translation

    Target ORF information:

    RefSeq Version NM_019294.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_019294.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGACCAG GCTCAGGCGA TGGAGACTCG 
    GACCAGAGCA GGAACCGACA AGGAACCCCC GTACCGGCCT CGGGGCCGGC GGCCGCCTAC
    AAGCAGTCAA AAGCGCAGAG GGCGACTATG GCTTTGTACA ACCCCATACC AGTCCGGCAG
    AACTGTTTCA CGGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AACTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCTTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGAAGAC TGGAGAAGAC AGAACCATAT TTTATTGGAA TCTTCTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCTCTAGG ATTCATCTTC CATAAGGGTT CGTACCTCCG TAACGGCTGG
    AATGTCATGG ACTTCATTGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCATTTC
    AACACCCACG TGGACCTGCG GACCCTCCGG GCTGTGCGTG TCCTGAGGCC CTTAAAGCTC
    GTGTCAGGAA TACCTAGCCT GCAGATCGTG CTGAAGTCCA TCATGAAGGC CATGGTGCCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATTCTCA TGTTTGCCAT CATTGGCTTG
    GAGTTCTACA GTGGCAAGTT GCATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAG
    GGATTTGATC CTCCTCACCC GTGTGGTGTA CAGGGCTGCC CGGCTGGTTA TGAATGTAAG
    GACTGGATCG GCCCCAATGA CGGGATCACC CAGTTTGACA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AATGCATCAC TATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA CTGGCTGTAC TTCATCCCCC TCATCATCAT CGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGCGTGCTT TCCGGGGAAT TTGCCAAAGA AAGAGAGAGA
    GTGGAGAACC GAAGAGCATT CATGAAGCTC CGGCGTCAGC AACAGATTGA GCGCGAGTTG
    AATGGCTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAATAAA
    AACTCGGGAA CATCAGCCTT GGAAGTGCTT CGAAGGGCAA CCATCAAAAG GAGCCGGACG
    GAGGCCATGA CCCGAGACTC CAGCGATGAG CACTGCGTTG ATATCTCTTC AGTGGGCACA
    CCTCTTGCCA GAGCCAGTAT CAAGAGCACA AAAGTAGACG GAGCCTCCTA TTTCCGGCAC
    AAGGAACGGC TTCTGCGTAT CTCTATCCGA CACATGGTCA AGTCGCAAGT GTTCTACTGG
    ATCGTCCTGA GTGTTGTGGC TCTCAATACT GCCTGTGTGG CCATCGTTCA TCACAACCAG
    CCACAGTGGC TCACTCACCT CCTCTACTAT GCAGAATTTT TGTTTCTGGG ACTCTTCCTG
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCACGCC TTTATTTTCA CTCATCCTTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGCATCTTTG AGGTCGTTTG GGCAATCTTC
    CGACCTGGCA CATCTTTTGG AATCAGTGTC TTACGAGCTC TCCGGCTCCT AAGGATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATCTGGTTG TTTCCTTGAT GAGCTCCATG
    AAGTCTATCA TCAGCTTACT CTTCCTCCTC TTCCTCTTCA TTGTTGTCTT TGCTCTCCTG
    GGAATGCAGC TCTTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCTAATTTT
    GATACCTTCC CTGCAGCTAT CATGACAGTG TTCCAGATCC TGACGGGGGA AGACTGGAAT
    GAAGTGCTGT ATAACGGCAT CCGCTCCCAG GGTGGGGTGA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGA AACTACACAC TGCTGAATGT ATTCTTGGCT
    ATCGCTGTGG ACAATCTCGC CAATGCCCAG GAACTGACCA AGGATGAGCA GGAGGAAGAA
    GAAGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCT
    CCCAACATGC CTTCCATTGA GAGGGAGAGG AGACGCCGAC ACCACATGTC TGTGTGGGAA
    CAGCGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA
    GAGGAGGCCC CACCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCA
    CTCAATGCTC ATCCTAGCCT CTATCGGAGG CCCAGGCCCA TTGAGGGCCT ACCCCTGGGC
    TTGGGTCTTG AAAAATGTGA AGAGGAGCGT ATCAGCCGTG GAGGTTCTCT CAAGGGGGAC
    ATAGGTGGCC TGACTAGCGT CCTGGACAAC CAGAGGAGCC CTTTGTCCCT GGGCAAACGG
    GAGCCACCGT GGCTGCCCAG ATCCTGTCAT GGAAACTGTG ACCCAACCCA GCAGGAGACT
    GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG
    CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGAGT CTGCCTCTGC CTCCAGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCATCGATGG GGAGAAGGAA
    CACGAGCCTC AGAGCAGCCA CAGGAGCAAG GAGCCAACCA TCCATGAAGA AGAGAGAACT
    CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCAGGACT GGTAGGAGCT
    CTTGATGAGG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT
    GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGCGAGC AAGCCCTGCT TGCGGATGTA
    CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCTG ACCTCTCCTG CATGACAACC
    AACATGGACA AGGCTACCAC TGAGAGCACC AGTGTCACAG TCGCCATCCC TGACGTGGAC
    CCCTTGGTGG ACTCAACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC
    TTGAAGGAAG CAGAGACCAA AGAGGAAGAG GAAGAGGTGG AAAAGAAGAA GCAGAAGAAG
    GAGAAGCGGG AGACTGGAAA AGCCATGGTG CCCCATAGCT CTATGTTCAT CTTCAGCACC
    ACTAACCCGA TCCGCAAGGC TTGCCACTAC ATTGTGAATC TACGCTACTT TGAGATGTGC
    ATTCTCTTGG TGATTGCGGC CAGCAGCATT GCCCTGGCAG CTGAGGACCC TGTACTAACT
    AACTCAGAGC GTAACAAAGT CCTGAGATAT TTCGACTATG TTTTCACAGG AGTGTTTACC
    TTTGAGATGG TGATAAAGAT GATAGACCAA GGCTTGATCC TGCAGGATGG ATCGTACTTC
    CGAGACCTCT GGAATATCCT GGACTTTGTG GTAGTGGTTG GTGCATTGGT AGCATTTGCT
    CTGGCGAACG CTTTGGGAAC CAACAAGGGG CGGGATATCA AGACGATCAA GTCTCTGAGG
    GTACTTCGAG TTCTGCGACC ACTGAAAACC ATCAAGCGCT TGCCCAAACT GAAGGCTGTG
    TTTGACTGCG TGGTGACCTC CTTGAAGAAT GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTTATGTTCA TCTTTGCCGT CATTGCTGTT CAGTTATTCA AGGGAAAGTT CTTTTATTGC
    ACCGACAGTT CCAAGGACAC AGAGAAGGAA TGCATAGGCA ACTATGTAGA CCACGAGAAA
    AACAAGATGG AGGTGAAGGG CCGGGAGTGG AAGCGTCATG AATTCCACTA TGACAACATC
    ATCTGGGCCC TGCTGACCCT CTTCACCGTC TCCACGGGGG AAGGATGGCC TCAGGTTCTG
    CAGCACTCGG TAGATGTGAC AGAGGAGGAC AGAGGCCCAA GCCGCAGCAA CCGCATGGAG
    ATGTCCATCT TCTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAATATCTTC
    GTGGCCCTCA TTATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA GGAGTGTAGC
    CTAGAAAAGA ATGAGCGGGC ATGCATCGAC TTCGCCATTA GTGCCAAACC TCTCACCCGC
    TACATGCCAC AGAACAGGCA CACCTTCCAG TACCGCGTGT GGCACTTCGT AGTCTCGCCA
    TCCTTTGAGT ATACCATCAT GGCCATGATT GCCTTGAACA CCGTTGTGCT GATGATGAAG
    TACTATTCTG CTCCTTGGAC CTATGAGCTC GCCCTAAAGT ACCTGAACAT CGCCTTCACT
    ATGGTGTTTT CCCTAGAATG TGTCCTAAAG GTCATCGCTT TCGGCTTCTT GAACTATTTC
    CGAGATACCT GGAACATCTT TGACTTCATT ACCGTGATCG GCAGTATCAC AGAAATTATC
    CTGACAGACA GCAAGCTGGT GAACACCAGT GGCTTCAATA TGAGCTTTCT GGAGCTCTTC
    CGAGCTGCAC GGCTCATAAA GCTTCTGCGA CAGGGCTACA CCATCCGCAT CTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCTCTCCCC TATGTCTGCC TTCTGATTGC CATGCTTTTC
    TTCATCTACG CTATCATTGG GATGCAGGTG TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CATATCAACC GGCACAACAA CTTTCGGAGT TTCTTCGGGT CTCTCATGCT GCTTTTCAGG
    AGCGCCACAG GGGAGGCTTG GCAGGAGATT ATGTTGTCAT GTCTGGGCGA GAAGGGCTGT
    GAGCCTGACA CCACTGCACC CTCAGGGCAG AACGAGAGTG AGCGCTGTGG CACTGACCTG
    GCCTACGTTT ATTTTGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTT
    GTGGCTGTTA TCATGGACAA CTTTGAGTAC CTGACTCGAG ATTCGTCCAT CCTGGGGCCT
    CACCACTTGG ATGAATTTGT CCGTGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCACCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAAGT GGCCTATAAG AGGTTGGTCC TGATGAATAT GCCAGTGGCT
    GAGGACATGA CAGTCCATTT CACCTCCACA CTTATGGCTC TGATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGTGG TGCAGACAGA CAGCAGCTAG ACTCAGAGCT ACAAAAAGAG
    ACCCTGGCCA TTTGGCCTCA CCTGTCCCAA AAGATGTTGG ATCTGCTAGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAGATC TATGCAGCAA TGATGATTAT GGACTACTAT
    AAACAGAGTA AGGTAAAGAA ACAGAGACAG CAGCTGGAAG AACAGAAAAA TGCACCCATG
    TTCCAGCGCA TGGAGCCCTC CTCACTGCCT CAGGAGATCA TTTCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC TGTTTCTGGC CTGAGTGGTC GGAGTGGATA CCCTTCAATG
    AGTCCACTCT CCCCTCAAGA AATATTCCAG TTGGCTTGTA TGGACCCAGC GGATGATGGA
    CAATTCCAGG AACAGCAATC GCTGGAGCCA GAGGTTAGTG AATTAAAAAG TGTGCAGTCC
    TCTAACCATG GCATCTACCT TCCTCCGGAC ACCCAGGAGC ATGCGGGATC TGGGAGGGCA
    TCCTCTATGC CACGTCTGAC TATGGATCCC CAGGTGGTAA CAGACCCTAG CTCTATGCGA
    CGTTCATTTT CTACAATTCG GGATAAGCGT TCAAATTCCT CCTGGTTGGA GGAGTTCTCC
    ATGGAACGTA GCAGTGAAAA CACCTACAAG TCACGACGCA GAAGTTACCA TTCCTCCCTG
    AGGCTGTCAG CCCACCGCCT GAATTCTGAC TCTGGTCACA AGTCTGACAC TCACCGCTCA
    GGAGGCAGGG AGAGAGGCCG ATCCAAAGAA CGAAAGCATC TCCTCTCTCC GGATGTCTCC
    CGCTGCAATT CTGAGGAGCG AGGAACCCAG GCTGACTGGG AGTCCCCAGA ACGTCGCCAG
    TCCAGGTCAC CCAGTGAGGG AAGGTCACAA ACCCCCAACA GACAGGGTAC AGGTTCACTG
    AGTGAGAGCT CCATTCCCTC TATCTCCGAC ACCAGCACCC CAAGACGAAG TCGTCGCCAG
    CTCCCACCTG TGCCACCAAA GCCTCGGCCC CTCCTCTCCT ACAGCTCCCT GATGAGACAC
    ACTGGCGGCA TCTCTCCACC TCCTGATGGA AGTGAGGGTG GATCCCCACT GGCCTCTCAA
    GCTCTGGAAA GCAACAGCGC TTGCCTAACC GAGTCTTCCA ACTCCTTGCA CCCCCAGCAG
    GGCCAGCACC CTTCCCCACA GCACTACATC TCTGAGCCCT ATCTGGCCCT CCATGAAGAC
    TCCCACGCCT CAGACTGTGG CGAGGAGGAG ACACTCACCT TTGAGGCAGC TGTAGCTACT
    AGCTTGGGCC GGTCCAATAC CATTGGCTCT GCCCCACCCC TACGGCACAG CTGGCAGATG
    CCTAATGGGC ACTATCGGCG GCGGAGGCTG GGGGGCCTGG GCCTGGCCAT GATGTGTGGA
    GCTGTCAGTG ACCTCCTGAG TGACACGGAA GAAGACGATA AGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa35498
    Clone ID Related Accession (Same CDS sequence) XM_017598908.1 , XM_017598909.1 , XM_006250069.3
    Accession Version XM_017598908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GTGGCCGCAT CCATTACACT GAGATGTATG AAATGCTGAC TCTCATGTCA
    CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CCTATAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGAGCCAGA GGTTAGTGAA
    TTAAAAAGTG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTCCGGACAC CCAGGAGCAT
    GCGGGATCTG GGAGGGCATC CTCTATGCCA CGTCTGACTA TGGATCCCCA GGTGGTAACA
    GACCCTAGCT CTATGCGACG TTCATTTTCT ACAATTCGGG ATAAGCGTTC AAATTCCTCC
    TGGTTGGAGG AGTTCTCCAT GGAACGTAGC AGTGAAAACA CCTACAAGTC ACGACGCAGA
    AGTTACCATT CCTCCCTGAG GCTGTCAGCC CACCGCCTGA ATTCTGACTC TGGTCACAAG
    TCTGACACTC ACCGCTCAGG AGGCAGGGAG AGAGGCCGAT CCAAAGAACG AAAGCATCTC
    CTCTCTCCGG ATGTCTCCCG CTGCAATTCT GAGGAGCGAG GAACCCAGGC TGACTGGGAG
    TCCCCAGAAC GTCGCCAGTC CAGGTCACCC AGTGAGGGAA GGTCACAAAC CCCCAACAGA
    CAGGGTACAG GTTCACTGAG TGAGAGCTCC ATTCCCTCTA TCTCCGACAC CAGCACCCCA
    AGACGAAGTC GTCGCCAGCT CCCACCTGTG CCACCAAAGC CTCGGCCCCT CCTCTCCTAC
    AGCTCCCTGA TGAGACACAC TGGCGGCATC TCTCCACCTC CTGATGGAAG TGAGGGTGGA
    TCCCCACTGG CCTCTCAAGC TCTGGAAAGC AACAGCGCTT GCCTAACCGA GTCTTCCAAC
    TCCTTGCACC CCCAGCAGGG CCAGCACCCT TCCCCACAGC ACTACATCTC TGAGCCCTAT
    CTGGCCCTCC ATGAAGACTC CCACGCCTCA GACTGTGGCG AGGAGGAGAC ACTCACCTTT
    GAGGCAGCCG TAGCTACTAG CTTGGGCCGG TCCAATACCA TTGGCTCTGC CCCACCCCTA
    CGGCACAGCT GGCAGATGCC TAATGGGCAC TATCGGCGGC GGAGGCGTGG GGGGCCTGGG
    CCTGGCATGA TGTGTGGAGC TGTCAGTGAC CTCCTGAGTG ACACGGAAGA AGACGATAAG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454397.1
    CDS561..7946
    Translation

    Target ORF information:

    RefSeq Version XM_017598908.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GTGGCCGCAT CCATTACACT GAGATGTATG AAATGCTGAC TCTCATGTCA
    CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CCTATAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGAGCCAGA GGTTAGTGAA
    TTAAAAAGTG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTCCGGACAC CCAGGAGCAT
    GCGGGATCTG GGAGGGCATC CTCTATGCCA CGTCTGACTA TGGATCCCCA GGTGGTAACA
    GACCCTAGCT CTATGCGACG TTCATTTTCT ACAATTCGGG ATAAGCGTTC AAATTCCTCC
    TGGTTGGAGG AGTTCTCCAT GGAACGTAGC AGTGAAAACA CCTACAAGTC ACGACGCAGA
    AGTTACCATT CCTCCCTGAG GCTGTCAGCC CACCGCCTGA ATTCTGACTC TGGTCACAAG
    TCTGACACTC ACCGCTCAGG AGGCAGGGAG AGAGGCCGAT CCAAAGAACG AAAGCATCTC
    CTCTCTCCGG ATGTCTCCCG CTGCAATTCT GAGGAGCGAG GAACCCAGGC TGACTGGGAG
    TCCCCAGAAC GTCGCCAGTC CAGGTCACCC AGTGAGGGAA GGTCACAAAC CCCCAACAGA
    CAGGGTACAG GTTCACTGAG TGAGAGCTCC ATTCCCTCTA TCTCCGACAC CAGCACCCCA
    AGACGAAGTC GTCGCCAGCT CCCACCTGTG CCACCAAAGC CTCGGCCCCT CCTCTCCTAC
    AGCTCCCTGA TGAGACACAC TGGCGGCATC TCTCCACCTC CTGATGGAAG TGAGGGTGGA
    TCCCCACTGG CCTCTCAAGC TCTGGAAAGC AACAGCGCTT GCCTAACCGA GTCTTCCAAC
    TCCTTGCACC CCCAGCAGGG CCAGCACCCT TCCCCACAGC ACTACATCTC TGAGCCCTAT
    CTGGCCCTCC ATGAAGACTC CCACGCCTCA GACTGTGGCG AGGAGGAGAC ACTCACCTTT
    GAGGCAGCCG TAGCTACTAG CTTGGGCCGG TCCAATACCA TTGGCTCTGC CCCACCCCTA
    CGGCACAGCT GGCAGATGCC TAATGGGCAC TATCGGCGGC GGAGGCGTGG GGGGCCTGGG
    CCTGGCATGA TGTGTGGAGC TGTCAGTGAC CTCCTGAGTG ACACGGAAGA AGACGATAAG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa35498
    Clone ID Related Accession (Same CDS sequence) XM_017598908.1 , XM_017598909.1 , XM_006250069.3
    Accession Version XM_017598909.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GTGGCCGCAT CCATTACACT GAGATGTATG AAATGCTGAC TCTCATGTCA
    CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CCTATAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGAGCCAGA GGTTAGTGAA
    TTAAAAAGTG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTCCGGACAC CCAGGAGCAT
    GCGGGATCTG GGAGGGCATC CTCTATGCCA CGTCTGACTA TGGATCCCCA GGTGGTAACA
    GACCCTAGCT CTATGCGACG TTCATTTTCT ACAATTCGGG ATAAGCGTTC AAATTCCTCC
    TGGTTGGAGG AGTTCTCCAT GGAACGTAGC AGTGAAAACA CCTACAAGTC ACGACGCAGA
    AGTTACCATT CCTCCCTGAG GCTGTCAGCC CACCGCCTGA ATTCTGACTC TGGTCACAAG
    TCTGACACTC ACCGCTCAGG AGGCAGGGAG AGAGGCCGAT CCAAAGAACG AAAGCATCTC
    CTCTCTCCGG ATGTCTCCCG CTGCAATTCT GAGGAGCGAG GAACCCAGGC TGACTGGGAG
    TCCCCAGAAC GTCGCCAGTC CAGGTCACCC AGTGAGGGAA GGTCACAAAC CCCCAACAGA
    CAGGGTACAG GTTCACTGAG TGAGAGCTCC ATTCCCTCTA TCTCCGACAC CAGCACCCCA
    AGACGAAGTC GTCGCCAGCT CCCACCTGTG CCACCAAAGC CTCGGCCCCT CCTCTCCTAC
    AGCTCCCTGA TGAGACACAC TGGCGGCATC TCTCCACCTC CTGATGGAAG TGAGGGTGGA
    TCCCCACTGG CCTCTCAAGC TCTGGAAAGC AACAGCGCTT GCCTAACCGA GTCTTCCAAC
    TCCTTGCACC CCCAGCAGGG CCAGCACCCT TCCCCACAGC ACTACATCTC TGAGCCCTAT
    CTGGCCCTCC ATGAAGACTC CCACGCCTCA GACTGTGGCG AGGAGGAGAC ACTCACCTTT
    GAGGCAGCCG TAGCTACTAG CTTGGGCCGG TCCAATACCA TTGGCTCTGC CCCACCCCTA
    CGGCACAGCT GGCAGATGCC TAATGGGCAC TATCGGCGGC GGAGGCGTGG GGGGCCTGGG
    CCTGGCATGA TGTGTGGAGC TGTCAGTGAC CTCCTGAGTG ACACGGAAGA AGACGATAAG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454398.1
    CDS34..7419
    Translation

    Target ORF information:

    RefSeq Version XM_017598909.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017598909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GTGGCCGCAT CCATTACACT GAGATGTATG AAATGCTGAC TCTCATGTCA
    CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CCTATAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGAGCCAGA GGTTAGTGAA
    TTAAAAAGTG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTCCGGACAC CCAGGAGCAT
    GCGGGATCTG GGAGGGCATC CTCTATGCCA CGTCTGACTA TGGATCCCCA GGTGGTAACA
    GACCCTAGCT CTATGCGACG TTCATTTTCT ACAATTCGGG ATAAGCGTTC AAATTCCTCC
    TGGTTGGAGG AGTTCTCCAT GGAACGTAGC AGTGAAAACA CCTACAAGTC ACGACGCAGA
    AGTTACCATT CCTCCCTGAG GCTGTCAGCC CACCGCCTGA ATTCTGACTC TGGTCACAAG
    TCTGACACTC ACCGCTCAGG AGGCAGGGAG AGAGGCCGAT CCAAAGAACG AAAGCATCTC
    CTCTCTCCGG ATGTCTCCCG CTGCAATTCT GAGGAGCGAG GAACCCAGGC TGACTGGGAG
    TCCCCAGAAC GTCGCCAGTC CAGGTCACCC AGTGAGGGAA GGTCACAAAC CCCCAACAGA
    CAGGGTACAG GTTCACTGAG TGAGAGCTCC ATTCCCTCTA TCTCCGACAC CAGCACCCCA
    AGACGAAGTC GTCGCCAGCT CCCACCTGTG CCACCAAAGC CTCGGCCCCT CCTCTCCTAC
    AGCTCCCTGA TGAGACACAC TGGCGGCATC TCTCCACCTC CTGATGGAAG TGAGGGTGGA
    TCCCCACTGG CCTCTCAAGC TCTGGAAAGC AACAGCGCTT GCCTAACCGA GTCTTCCAAC
    TCCTTGCACC CCCAGCAGGG CCAGCACCCT TCCCCACAGC ACTACATCTC TGAGCCCTAT
    CTGGCCCTCC ATGAAGACTC CCACGCCTCA GACTGTGGCG AGGAGGAGAC ACTCACCTTT
    GAGGCAGCCG TAGCTACTAG CTTGGGCCGG TCCAATACCA TTGGCTCTGC CCCACCCCTA
    CGGCACAGCT GGCAGATGCC TAATGGGCAC TATCGGCGGC GGAGGCGTGG GGGGCCTGGG
    CCTGGCATGA TGTGTGGAGC TGTCAGTGAC CTCCTGAGTG ACACGGAAGA AGACGATAAG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa35498
    Clone ID Related Accession (Same CDS sequence) XM_017598908.1 , XM_017598909.1 , XM_006250069.3
    Accession Version XM_006250069.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250069.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GTGGCCGCAT CCATTACACT GAGATGTATG AAATGCTGAC TCTCATGTCA
    CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CCTATAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGAGCCAGA GGTTAGTGAA
    TTAAAAAGTG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTCCGGACAC CCAGGAGCAT
    GCGGGATCTG GGAGGGCATC CTCTATGCCA CGTCTGACTA TGGATCCCCA GGTGGTAACA
    GACCCTAGCT CTATGCGACG TTCATTTTCT ACAATTCGGG ATAAGCGTTC AAATTCCTCC
    TGGTTGGAGG AGTTCTCCAT GGAACGTAGC AGTGAAAACA CCTACAAGTC ACGACGCAGA
    AGTTACCATT CCTCCCTGAG GCTGTCAGCC CACCGCCTGA ATTCTGACTC TGGTCACAAG
    TCTGACACTC ACCGCTCAGG AGGCAGGGAG AGAGGCCGAT CCAAAGAACG AAAGCATCTC
    CTCTCTCCGG ATGTCTCCCG CTGCAATTCT GAGGAGCGAG GAACCCAGGC TGACTGGGAG
    TCCCCAGAAC GTCGCCAGTC CAGGTCACCC AGTGAGGGAA GGTCACAAAC CCCCAACAGA
    CAGGGTACAG GTTCACTGAG TGAGAGCTCC ATTCCCTCTA TCTCCGACAC CAGCACCCCA
    AGACGAAGTC GTCGCCAGCT CCCACCTGTG CCACCAAAGC CTCGGCCCCT CCTCTCCTAC
    AGCTCCCTGA TGAGACACAC TGGCGGCATC TCTCCACCTC CTGATGGAAG TGAGGGTGGA
    TCCCCACTGG CCTCTCAAGC TCTGGAAAGC AACAGCGCTT GCCTAACCGA GTCTTCCAAC
    TCCTTGCACC CCCAGCAGGG CCAGCACCCT TCCCCACAGC ACTACATCTC TGAGCCCTAT
    CTGGCCCTCC ATGAAGACTC CCACGCCTCA GACTGTGGCG AGGAGGAGAC ACTCACCTTT
    GAGGCAGCCG TAGCTACTAG CTTGGGCCGG TCCAATACCA TTGGCTCTGC CCCACCCCTA
    CGGCACAGCT GGCAGATGCC TAATGGGCAC TATCGGCGGC GGAGGCGTGG GGGGCCTGGG
    CCTGGCATGA TGTGTGGAGC TGTCAGTGAC CTCCTGAGTG ACACGGAAGA AGACGATAAG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006250131.1
    CDS885..8270
    Translation

    Target ORF information:

    RefSeq Version XM_006250069.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus calcium voltage-gated channel subunit alpha1 E (Cacna1e), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006250069.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC TGCCCTGTGC 
    CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGTTGGCA GGCGCCGCCA CGTCCTGGCT
    CTGCCTGAGG AGGAGGCATG GCGGCCGCAG CAGTGTGCCC TGCTCGAGGC CGATGCCTCC
    GACGAGGTGG GCATGCTGCC TGCTTCCCCG CGCTCTGCGG TGTCCGGCTT CGACAACTTC
    TTCCCGGTGC AGGAGGGCGA GGGCCCAGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC
    TCCAGTCCCT TCCTCCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC
    GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC
    CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGACCAGGC
    TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT ACCGGCCTCG
    GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC
    AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA
    GAAGATAACA TTGTCAGGAA ATATGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC
    ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT
    GAGGATGACA AGACCCCAAT GTCCCGAAGA CTGGAGAAGA CAGAACCATA TTTTATTGGA
    ATCTTCTGCT TTGAAGCTGG GATCAAAATC GTGGCTCTAG GATTCATCTT CCATAAGGGT
    TCGTACCTCC GTAACGGCTG GAATGTCATG GACTTCATTG TGGTCCTCAG TGGCATCCTG
    GCCACTGCAG GAACCCATTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT
    GTCCTGAGGC CCTTAAAGCT CGTGTCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCC
    ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATTCTC
    ATGTTTGCCA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG
    AACAATTCAG GTATTCTAGA GGGATTTGAT CCTCCTCACC CGTGTGGTGT ACAGGGCTGC
    CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC
    AACATCCTTT TTGCTGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT
    GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCC
    CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTTGTCC TGGGCGTGCT TTCCGGGGAA
    TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCAT TCATGAAGCT CCGGCGTCAG
    CAACAGATTG AGCGCGAGTT GAATGGCTAC CGCGCCTGGA TAGACAAAGC AGAGGAAGTC
    ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA
    ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT
    GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT
    GGAGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGTA TCTCTATCCG ACACATGGTC
    AAGTCGCAAG TGTTCTACTG GATCGTCCTG AGTGTTGTGG CTCTCAATAC TGCCTGTGTG
    GCCATCGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAATTT
    TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC
    CTTTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT
    GAGGTCGTTT GGGCAATCTT CCGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT
    CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT
    GTTTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTCCT CTTCCTCTTC
    ATTGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT
    GGGACTCCTT CGGCTAATTT TGATACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC
    CTGACGGGGG AAGACTGGAA TGAAGTGATG TATAACGGCA TCCGCTCCCA GGGTGGGGTG
    AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACA
    CTGCTGAATG TATTCTTGGC TATCGCTGTG GACAATCTCG CCAATGCCCA GGAACTGACC
    AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG
    GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA
    CACCACATGT CGATGTGGGA GCCGCGCAGC AGCCACCTGA GGGAGAGGAG ACGCCGACAC
    CACATGTCTG TGTGGGAACA GCGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC
    CAAGAAGCCC TCAACAAAGA GGAGGCCCCA CCAATGAACC CCCTCAATCC ACTCAACCCA
    CTGAGTCCTC TCAACCCACT CAATGCTCAT CCTAGCCTCT ATCGGAGGCC CAGGCCCATT
    GAGGGCCTAG CCCTGGGCTT GGGTCTTGAA AAATGTGAAG AGGAGCGTAT CAGCCGTGGA
    GGTTCTCTCA AGGGGGACAT AGGTGGCCTG ACTAGCGTCC TGGACAACCA GAGGAGCCCT
    TTGTCCCTGG GCAAACGGGA GCCACCGTGG CTGCCCAGAT CCTGTCATGG AAACTGTGAC
    CCAACCCAGC AGGAGACTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG
    CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGAGTCT
    GCCTCTGCCT CCAGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC
    ATCGATGGGG AGAAGGAACA CGAGCCTCAG AGCAGCCACA GGAGCAAGGA GCCAACCATC
    CATGAAGAAG AGAGAACTCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC
    TCAGGACTGG TAGGAGCTCT TGATGAGGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG
    CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGCGAGCAA
    GCCCTGCTTG CGGATGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCTGAC
    CTCTCCTGCA TGACAACCAA CATGGACAAG GCTACCACTG AGAGCACCAG TGTCACAGTC
    GCCATCCCTG ACATGGACCC CTTGGTGGAC TCAACAGTGG TGAACATTAG CAACAAGACG
    GATGGAGAAG CCAGTCCCTT GAAGGAAGCA GAGACCAAAG AGGAAGAGGA AGAGGTGGAA
    AAGAAGAAGC AGAAGAAGGA GAAGCGGGAG ACTGGAAAAG CCATGGTGCC CCATAGCTCT
    ATGTTCATCT TCAGCACCAC TAACCCGATC CGCAAGGCTT GCCACTACAT TGTGAATCTA
    CGCTACTTTG AGATGTGCAT TCTCTTGGTG ATTGCGGCCA GCAGCATTGC CCTGGCAGCT
    GAGGACCCTG TACTAACTAA CTCAGAGCGT AACAAAGTCC TGAGATATTT CGACTATGTT
    TTCACAGGAG TGTTTACCTT TGAGATGGTG ATAAAGATGA TAGACCAAGG CTTGATCCTG
    CAGGATGGAT CGTACTTCCG AGACCTCTGG AATATCCTGG ACTTTGTGGT AGTGGTTGGT
    GCATTGGTAG CATTTGCTCT GGCGAACGCT TTGGGAACCA ACAAGGGGCG GGATATCAAG
    ACGATCAAGT CTCTGAGGGT ACTTCGAGTT CTGCGACCAC TGAAAACCAT CAAGCGCTTG
    CCCAAACTGA AGGCTGTGTT TGACTGCGTG GTGACCTCCT TGAAGAATGT CTTCAACATA
    CTCATTGTCT ACAAGCTCTT TATGTTCATC TTTGCCGTCA TTGCTGTTCA GTTATTCAAG
    GGAAAGTTCT TTTATTGCAC CGACAGTTCC AAGGACACAG AGAAGGAATG CATAGGCAAC
    TATGTAGACC ACGAGAAAAA CAAGATGGAG GTGAAGGGCC GGGAGTGGAA GCGTCATGAA
    TTCCACTATG ACAACATCAT CTGGGCCCTG CTGACCCTCT TCACCGTCTC CACGGGGGAA
    GGATGGCCTC AGGTTCTGCA GCACTCGGTA GATGTGACAG AGGAGGACAG AGGCCCAAGC
    CGCAGCAACC GCATGGAGAT GTCCATCTTC TATGTGGTCT ACTTTGTGGT CTTCCCTTTC
    TTCTTTGTCA ATATCTTCGT GGCCCTCATT ATCATCACCT TCCAGGAGCA AGGGGACAAG
    ATGATGGAGG AGTGTAGCCT AGAAAAGAAT GAGCGGGCAT GCATCGACTT CGCCATTAGT
    GCCAAACCTC TCACCCGCTA CATGCCACAA AACAGGCACA CCTTCCAGTA CCGCGTGTGG
    CACTTCGTAG TCTCGCCATC CTTTGAGTAT ACCATCATGG CCATGATTGC CTTGAACACC
    GTTGTGCTGA TGATGAAGTA CTATTCTGCT CCTTGGACCT ATGAGCTCGC CCTAAAGTAC
    CTGAACATCG CCTTCACTAT GGTGTTTTCC CTAGAATGTG TCCTAAAGGT CATCGCTTTC
    GGCTTCTTGA ACTATTTCCG AGATACCTGG AACATCTTTG ACTTCATTAC CGTGATCGGC
    AGTATCACAG AAATTATCCT GACAGACAGC AAGCTGGTGA ACACCAGTGG CTTCAATATG
    AGCTTTCTGA AGCTCTTCCG AGCTGCACGG CTCATAAAGC TTCTGCGACA GGGCTACACC
    ATCCGCATCT TACTGTGGAC CTTCGTGCAG TCCTTTAAGG CTCTCCCCTA TGTCTGCCTT
    CTGATTGCCA TGCTTTTCTT CATCTACGCT ATCATTGGGA TGCAGGTGTT TGGAAACATA
    AAGTTAGATG AGGAGAGTCA TATCAACCGG CACAACAACT TTCGGAGTTT CTTCGGGTCT
    CTCATGCTGC TTTTCAGGAG CGCCACAGGG GAGGCTTGGC AGGAGATTAT GTTGTCATGT
    CTGGGCGAGA AGGGCTGTGA GCCTGACACC ACTGCACCCT CAGGGCAGAA CGAGAGTGAG
    CGCTGTGGCA CTGACCTGGC CTACGTTTAT TTTGTCTCCT TCATCTTCTT CTGCTCCTTC
    CTGATGCTCA ACCTGTTTGT GGCTGTTATC ATGGACAACT TTGAGTACCT GACTCGAGAT
    TCGTCCATCC TGGGGCCTCA CCACTTGGAT GAATTTGTCC GTGTCTGGGC AGAGTATGAC
    AGAGCAGCAT GTGGCCGCAT CCATTACACT GAGATGTATG AAATGCTGAC TCTCATGTCA
    CCACCGCTAG GCCTCGGCAA GAGATGTCCC TCCAAAGTGG CCTATAAGAG GTTGGTCCTG
    ATGAATATGC CAGTGGCTGA GGACATGACA GTCCATTTCA CCTCCACACT TATGGCTCTG
    ATCCGGACAG CTCTGGACAT TAAAATCGCC AAAGGTGGTG CAGACAGACA GCAGCTAGAC
    TCAGAGCTAC AAAAAGAGAC CCTGGCCATT TGGCCTCACC TGTCCCAAAA GATGTTGGAT
    CTGCTAGTGC CCATGCCCAA AGCCTCTGAC CTGACTGTGG GCAAGATCTA TGCAGCAATG
    ATGATTATGG ACTACTATAA ACAGAGTAAG GTAAAGAAAC AGAGACAGCA GCTGGAAGAA
    CAGAAAAATG CACCCATGTT CCAGCGCATG GAGCCCTCCT CACTGCCTCA GGAGATCATT
    TCTAATGCCA AAGCCCTGCC TTATCTCCAG CAGGACCCTG TTTCTGGCCT GAGTGGTCGG
    AGTGGATACC CTTCAATGAG TCCACTCTCC CCTCAAGAAA TATTCCAGTT GGCTTGTATG
    GACCCAGCGG ATGATGGACA ATTCCAGGAA CAGCAATCGC TGGAGCCAGA GGTTAGTGAA
    TTAAAAAGTG TGCAGTCCTC TAACCATGGC ATCTACCTTC CTCCGGACAC CCAGGAGCAT
    GCGGGATCTG GGAGGGCATC CTCTATGCCA CGTCTGACTA TGGATCCCCA GGTGGTAACA
    GACCCTAGCT CTATGCGACG TTCATTTTCT ACAATTCGGG ATAAGCGTTC AAATTCCTCC
    TGGTTGGAGG AGTTCTCCAT GGAACGTAGC AGTGAAAACA CCTACAAGTC ACGACGCAGA
    AGTTACCATT CCTCCCTGAG GCTGTCAGCC CACCGCCTGA ATTCTGACTC TGGTCACAAG
    TCTGACACTC ACCGCTCAGG AGGCAGGGAG AGAGGCCGAT CCAAAGAACG AAAGCATCTC
    CTCTCTCCGG ATGTCTCCCG CTGCAATTCT GAGGAGCGAG GAACCCAGGC TGACTGGGAG
    TCCCCAGAAC GTCGCCAGTC CAGGTCACCC AGTGAGGGAA GGTCACAAAC CCCCAACAGA
    CAGGGTACAG GTTCACTGAG TGAGAGCTCC ATTCCCTCTA TCTCCGACAC CAGCACCCCA
    AGACGAAGTC GTCGCCAGCT CCCACCTGTG CCACCAAAGC CTCGGCCCCT CCTCTCCTAC
    AGCTCCCTGA TGAGACACAC TGGCGGCATC TCTCCACCTC CTGATGGAAG TGAGGGTGGA
    TCCCCACTGG CCTCTCAAGC TCTGGAAAGC AACAGCGCTT GCCTAACCGA GTCTTCCAAC
    TCCTTGCACC CCCAGCAGGG CCAGCACCCT TCCCCACAGC ACTACATCTC TGAGCCCTAT
    CTGGCCCTCC ATGAAGACTC CCACGCCTCA GACTGTGGCG AGGAGGAGAC ACTCACCTTT
    GAGGCAGCCG TAGCTACTAG CTTGGGCCGG TCCAATACCA TTGGCTCTGC CCCACCCCTA
    CGGCACAGCT GGCAGATGCC TAATGGGCAC TATCGGCGGC GGAGGCGTGG GGGGCCTGGG
    CCTGGCATGA TGTGTGGAGC TGTCAGTGAC CTCCTGAGTG ACACGGAAGA AGACGATAAG
    TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.