gRNAs
The following Plin5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plin5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa49510 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008766770.1 | |
| Accession Version | XM_008766770.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | perilipin-5 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGACCAGA GAGGCAAAGA CACCACCCTA GCTCTACACA GCAGGATGTC CGGTGATCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008764992.1 |
| CDS | 157..1548 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_008766770.1 |
1 | ATGGACCAGA GAGGCAAAGA CACCACCCTA GCTCTACACA GCAGGATGTC CGGTGATCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa13843 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001134637.1 | |
| Accession Version | NM_001134637.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | perilipin-5 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474092.1. On or before Sep 4, 2008 this sequence version replaced XM_001061544.1, XM_576698.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689600 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGACCAGA GAGGCAAAGA CACCACCCTA GCTCTACACA GCAGGATGTC CGGTGATCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001128109.1 |
| CDS | 114..1541 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001134637.1 |
1 | ATGGACCAGA GAGGCAAAGA CACCACCCTA GCTCTACACA GCAGGATGTC CGGTGATCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Perilipin 5 mediated lipid droplet remodelling revealed by coherent Raman imaging. |
A maternal high fat diet has long-lasting effects on skeletal muscle lipid and PLIN protein content in rat offspring at young adulthood. |
Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation. |
Overexpression of PLIN5 in skeletal muscle promotes oxidative gene expression and intramyocellular lipid content without compromising insulin sensitivity. |
Skeletal muscle PLIN proteins, ATGL and CGI-58, interactions at rest and following stimulated contraction. |
