gRNAs
The following Eefsec gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eefsec cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa09430 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001109249.1 | |
| Accession Version | NM_001109249.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-27 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | selenocysteine-specific elongation factor | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473957.1. On or before Oct 4, 2007 this sequence version replaced XM_575603.2, XM_001075510.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCGGGCC GGCGGGTAAA TGTGAATGTG GGTGTGCTGG GTCATATCGA TAGCGGCAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001102719.1 |
| CDS | 44..1135 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001109249.1 |
1 | ATGGCGGGCC GGCGGGTAAA TGTGAATGTG GGTGTGCTGG GTCATATCGA TAGCGGCAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa27456 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006236864.3 | |
| Accession Version | XM_006236864.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | selenocysteine-specific elongation factor isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236864.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGGCC GGCGGGTAAA TGTGAATGTG GGTGTGCTGG GTCATATCGA TAGCGGCAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006236926.1 |
| CDS | 60..1811 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006236864.3 |
1 | ATGGCGGGCC GGCGGGTAAA TGTGAATGTG GGTGTGCTGG GTCATATCGA TAGCGGCAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa60986 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017592829.1 | |
| Accession Version | XM_017592829.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | selenocysteine-specific elongation factor isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAGACCG ATCCTCTCCA TTCAGGTGCC CAGATCATTG ATTTGATGAT GCTCGTGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017448318.1 |
| CDS | 2203..3705 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017592829.1 |
1 | ATGAAGACCG ATCCTCTCCA TTCAGGTGCC CAGATCATTG ATTTGATGAT GCTCGTGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The selenocysteine-specific elongation factor contains a novel and multi-functional domain. |
Supramolecular complexes mediate selenocysteine incorporation in vivo. |
Thymic lymphomas arising in Msh2 deficient mice display a large increase in mutation frequency and an altered mutational spectrum. |
Somatic Apc mutations are selected upon their capacity to inactivate the beta-catenin downregulating activity. |
Decoding apparatus for eukaryotic selenocysteine insertion. |
