Pum3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pum3
Entrez Gene ID	499339
Full Name	pumilio RNA-binding family member 3
General protein information	Preferred Names pumilio RNA-binding family member 3 Names pumilio homolog 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q52

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025036.1	NP_001020207.1	pumilio homolog 3
XM_006231246.3	XP_006231308.1	pumilio homolog 3 isoform X1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR342C	NP_985889.1
Kluyveromyces lactis	KLLA0C12331g	XP_452748.1
Rattus norvegicus (Norway rat)	RGD1308750	XP_215276.4
Schizosaccharomyces pombe (fission yeast)	puf6	NP_588564.1
Neurospora crassa	NCU09380	XP_963826.1
Rattus norvegicus (Norway rat)	LOC100912029	XP_003751172.1
Rattus norvegicus (Norway rat)	LOC499339	NP_001020207.1
Saccharomyces cerevisiae (baker's yeast)	PUF6	NP_010784.1
Magnaporthe oryzae (rice blast fungus)	MGG_13803	XP_003710911.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

hPuf-A/KIAA0020 modulates PARP-1 cleavage upon genotoxic stress.
Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, Chang MS
Cancer research71(3)1126-34(2011 Feb)

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Ohta S, Bukowski-Wills JC, Sanchez-Pulido L, Alves Fde L, Wood L, Chen ZA, Platani M, Fischer L, Hudson DF, Ponting CP, Fukagawa T, Earnshaw WC, Rappsilber J
Cell142(5)810-21(2010 Sep)

A visual intracellular classification strategy for uncharacterized human proteins.
Hoja MR, Wahlestedt C, H??g C
Experimental cell research259(1)239-46(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pum3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pum3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07115	NM_001025036.1 Latest version!	Rattus norvegicus pumilio RNA-binding family member 3 (Pum3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa07115	XM_006231246.3 Latest version!	Rattus norvegicus pumilio RNA-binding family member 3 (Pum3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pum3 ( NM_001025036.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020207.1 Rattus norvegicus pumilio RNA-binding family member 3 (Pum3), mRNA.

Price & Availability↑

CloneID	ORa07115
Clone ID Related Accession (Same CDS sequence)	NM_001025036.1 , XM_006231246.3
Accession Version	NM_001025036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	pumilio homolog 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC092579.1. On Jun 25, 2005 this sequence version replaced XM_574647.1. ##Evidence-Data-START## Transcript exon combination :: BC092579.1, BC098744.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGTCA AAGGGAAGAA AAAAATTACA GGAAAGAGTC CACAGACATC ACAAGGAAAG 
AATAAATTTC ATAAAAACAG TGAGTCTAGT TCTGCAAAGG CATTCCCAAG GAAAGCTGCT 
AAGGAAGGCG GGCCTAAAGT CACATCTAAG AACTTTGAGA AAGGTGCCAC AAAACCTGGC 
AAGAAGCGTG TGAAGCAGTT CAAGAACAAG CCACAAGGGG GCAAAGGACC GAAGGACAAA 
TTCCAGAAGG CAAATAAATT CAACAAGAAG CGGAAATTCC AGCCAGATGG GAAAAGTGAT 
GAATCAGCAG CCAAGAAACC CAAGTGGGAT GACTTCAAAA AGAAGAAGAA AGAGCTGAAG 
CAGAACAGAC AGCTCAGTGA TAAGACCAAT TACGACGTCG TGGTTCGAGC AAAGCACATT 
TGGGAGAGCT TGAGAAGAAA AGACTGTGAC AAAGAAAAAA GGGTGAAGCT CATGAGTGAT 
TTGCAGAAGC TGATTCAAGG AAAAATTAAA ACTATTGCCT TTGCGCATGA CTCAACTCGA 
GTGATCCAGT GCCTCATTCA GTATGGGAGC GAGGAGCAGA GGAAGTGGGC TTTCGAAGAA 
TTGCAAGGTG ACTTGGTTGA ATTAAGTAAA GCCAAATATT CCAGGAATAT CGTTAAGAAA 
TTTCTCATGT ATGGAAGTAA ACCACAGATT GCGGAGATAA TCAGAAGTTT TAAAGGTCAT 
GTGAGGAAGA TGCTGCGGCA TTCAGAGGCA TCGGCCATTG TGGAGTATGC GTACAACGAC 
AAAGCCATTT TGGAACAGAG GAACATGCTG ACAGAGGAGC TCTATGGGAA CACATTTCAG 
CTTTACAAGT CAGCAGATCA CCCAACGCTG GAAAAGGTGC TAGAGGTGCA GCCAGGGAAG 
CTAGAACTTA TCCTGGATGA GATGAAGCAG ATTCTGACTC CCATGGCCCA AAAGGAAGCT 
GTGATTAAGC ACTCACTGGT TCATAAAGTA TTCTTGGACT TTTTTACCTA TGCACCCCCA 
AAACTTCGAT CAGAACTAAT TGAAGCCATC CGGGAAGCAG TGGTGTACCT GGCCCACACA 
CACGATGGCG CCAGAGTGGC CATGCATTGC TTGTGGCATG GTACACCCAA GGACAGGAAA 
GTGATTGTGA AAACAATGAA GACGTATGTG GAGAAGATTG CTAATGGCCA GTACTCTCAC 
CTGGTTCTCC TGGCAGCGTT CGATTGTATT GATGATACCA AGCTTGTGAA GCAGATAATC 
ATATCAGAAG TCATCAGCTC CTTGCCCAGC ATCGTTAATG ACAAATATGG AAGGAAAGTG 
CTGCTGTACC TGCTGAGCCC CCGAGCCCCC GCCCACTTGG TGCCAGAGAT CATCCAGCTG 
CTGCAGAAGG GCGACGGCAA TGCACACAGT AAGAAGGACA CTGCAATCCG CCGGCGTGAG 
CTCTTGGAGT CCATCTCTCC AGCTTTGCTG AGCTACCTGC AAGGACACAC TCGGGAGGTG 
GTGCTGGACA AGTCCGTGTG TGTGCTGGTG TCTGACATCC TGGGATCTGC TACTGGGGAC 
GCTCAGCCTG CTATGGATGC CATCGCCGGT TTGGCAGCAG AGGAACTGTA TCCTGGGGGC 
AAGGACGGAG AGCTCCACAT TGCTGAGCAT CCTGCAGGAC ATCTGGTCCT CAAATGGTTA 
ATAGAGCAAG ATAAAAAGAT AAAAGAAAAT GGAAAAGAAG GTTGTTTTGC AAAAACCCTT 
GTAGAGTGCG TTGGTATGAA GAACCTCAAG TCCTGGGCCA GCATCAACCG AGGAGCCATC 
GTTCTTTCCA GCCTTCTTCA GAGTTGTGAC CAGGACGTTG TAAACAAAGT CAAAGCTGGA 
CTGAAAACCC TGATTCCTAC CTTGGAAAAA ACCAAAAGCA CCAGCAAAGG CATCCAGACT 
CTGCTTGAGA AGTTGACTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020207.1
CDS	45..1988
Translation	MEVKGKKKITGKSPQTSQGKNKFHKNSESSSAKAFPRKAAKEGGPKVTSKNFEKGATKPGKKRVKQFKNKPQGGKGPKDKFQKANKFNKKRKFQPDGKSDESAAKKPKWDDFKKKKKELKQNRQLSDKTNYDVVVRAKHIWESLRRKDCDKEKRVKLMSDLQKLIQGKIKTIAFAHDSTRVIQCLIQYGSEEQRKWAFEELQGDLVELSKAKYSRNIVKKFLMYGSKPQIAEIIRSFKGHVRKMLRHSEASAIVEYAYNDKAILEQRNMLTEELYGNTFQLYKSADHPTLEKVLEVQPGKLELILDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKLRSELIEAIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKIANGQYSHLVLLAAFDCIDDTKLVKQIIISEVISSLPSIVNDKYGRKVLLYLLSPRAPAHLVPEIIQLLQKGDGNAHSKKDTAIRRRELLESISPALLSYLQGHTREVVLDKSVCVLVSDILGSATGDAQPAMDAIAGLAAEELYPGGKDGELHIAEHPAGHLVLKWLIEQDKKIKENGKEGCFAKTLVECVGMKNLKSWASINRGAIVLSSLLQSCDQDVVNKVKAGLKTLIPTLEKTKSTSKGIQTLLEKLTA

Target ORF information:

RefSeq Version	NM_001025036.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pumilio RNA-binding family member 3 (Pum3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025036.1

ORF Insert Sequence:

ATGGAAGTCA AAGGGAAGAA AAAAATTACA GGAAAGAGTC CACAGACATC ACAAGGAAAG 
AATAAATTTC ATAAAAACAG TGAGTCTAGT TCTGCAAAGG CATTCCCAAG GAAAGCTGCT 
AAGGAAGGCG GGCCTAAAGT CACATCTAAG AACTTTGAGA AAGGTGCCAC AAAACCTGGC 
AAGAAGCGTG TGAAGCAGTT CAAGAACAAG CCACAAGGGG GCAAAGGACC GAAGGACAAA 
TTCCAGAAGG CAAATAAATT CAACAAGAAG CGGAAATTCC AGCCAGATGG GAAAAGTGAT 
GAATCAGCAG CCAAGAAACC CAAGTGGGAT GACTTCAAAA AGAAGAAGAA AGAGCTGAAG 
CAGAACAGAC AGCTCAGTGA TAAGACCAAT TACGACGTCG TGGTTCGAGC AAAGCACATT 
TGGGAGAGCT TGAGAAGAAA AGACTGTGAC AAAGAAAAAA GGGTGAAGCT CATGAGTGAT 
TTGCAGAAGC TGATTCAAGG AAAAATTAAA ACTATTGCCT TTGCGCATGA CTCAACTCGA 
GTGATCCAGT GCCTCATTCA GTATGGGAGC GAGGAGCAGA GGAAGTGGGC TTTCGAAGAA 
TTGCAAGGTG ACTTGGTTGA ATTAAGTAAA GCCAAATATT CCAGGAATAT CGTTAAGAAA 
TTTCTCATGT ATGGAAGTAA ACCACAGATT GCGGAGATAA TCAGAAGTTT TAAAGGTCAT 
GTGAGGAAGA TGCTGCGGCA TTCAGAGGCA TCGGCCATTG TGGAGTATGC GTACAACGAC 
AAAGCCATTT TGGAACAGAG GAACATGCTG ACAGAGGAGC TCTATGGGAA CACATTTCAG 
CTTTACAAGT CAGCAGATCA CCCAACGCTG GAAAAGGTGC TAGAGGTGCA GCCAGGGAAG 
CTAGAACTTA TCCTGGATGA GATGAAGCAG ATTCTGACTC CCATGGCCCA AAAGGAAGCT 
GTGATTAAGC ACTCACTGGT TCATAAAGTA TTCTTGGACT TTTTTACCTA TGCACCCCCA 
AAACTTCGAT CAGAACTAAT TGAAGCCATC CGGGAAGCAG TGGTGTACCT GGCCCACACA 
CACGATGGCG CCAGAGTGGC CATGCATTGC TTGTGGCATG GTACACCCAA GGACAGGAAA 
GTGATTGTGA AAACAATGAA GACGTATGTG GAGAAGATTG CTAATGGCCA GTACTCTCAC 
CTGGTTCTCC TGGCAGCGTT CGATTGTATT GATGATACCA AGCTTGTGAA GCAGATAATC 
ATATCAGAAG TCATCAGCTC CTTGCCCAGC ATCGTTAATG ACAAATATGG AAGGAAAGTG 
CTGCTGTACC TGCTGAGCCC CCGAGCCCCC GCCCACTTGG TGCCAGAGAT CATCCAGCTG 
CTGCAGAAGG GCGACGGCAA TGCACACAGT AAGAAGGACA CTGCAATCCG CCGGCGTGAG 
CTCTTGGAGT CCATCTCTCC AGCTTTGCTG AGCTACCTGC AAGGACACAC TCGGGAGGTG 
GTGCTGGACA AGTCCGTGTG TGTGCTGGTG TCTGACATCC TGGGATCTGC TACTGGGGAC 
GCTCAGCCTG CTATGGATGC CATCGCCGGT TTGGCAGCAG AGGAACTGTA TCCTGGGGGC 
AAGGACGGAG AGCTCCACAT TGCTGAGCAT CCTGCAGGAC ATCTGGTCCT CAAATGGTTA 
ATAGAGCAAG ATAAAAAGAT AAAAGAAAAT GGAAAAGAAG GTTGTTTTGC AAAAACCCTT 
GTAGAGTGCG TTGGTATGAA GAACCTCAAG TCCTGGGCCA GCATCAACCG AGGAGCCATC 
GTTCTTTCCA GCCTTCTTCA GAGTTGTGAC CAGGACGTTG TAAACAAAGT CAAAGCTGGA 
CTGAAAACCC TGATTCCTAC CTTGGAAAAA ACCAAAAGCA CCAGCAAAGG CATCCAGACT 
CTGCTTGAGA AGTTGACTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pum3 ( XM_006231246.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231308.1 Rattus norvegicus pumilio RNA-binding family member 3 (Pum3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07115
Clone ID Related Accession (Same CDS sequence)	NM_001025036.1 , XM_006231246.3
Accession Version	XM_006231246.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pumilio homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCA AAGGGAAGAA AAAAATTACA GGAAAGAGTC CACAGACATC ACAAGGAAAG 
AATAAATTTC ATAAAAACAG TGAGTCTAGT TCTGCAAAGG CATTCCCAAG GAAAGCTGCT 
AAGGAAGGCG GGCCTAAAGT CACATCTAAG AACTTTGAGA AAGGTGCCAC AAAACCTGGC 
AAGAAGCGTG TGAAGCAGTT CAAGAACAAG CCACAAGGGG GCAAAGGACC GAAGGACAAA 
TTCCAGAAGG CAAATAAATT CAACAAGAAG CGGAAATTCC AGCCAGATGG GAAAAGTGAT 
GAATCAGCAG CCAAGAAACC CAAGTGGGAT GACTTCAAAA AGAAGAAGAA AGAGCTGAAG 
CAGAACAGAC AGCTCAGTGA TAAGACCAAT TACGACGTCG TGGTTCGAGC AAAGCACATT 
TGGGAGAGCT TGAGAAGAAA AGACTGTGAC AAAGAAAAAA GGGTGAAGCT CATGAGTGAT 
TTGCAGAAGC TGATTCAAGG AAAAATTAAA ACTATTGCCT TTGCGCATGA CTCAACTCGA 
GTGATCCAGT GCCTCATTCA GTATGGGAGC GAGGAGCAGA GGAAGTGGGC TTTCGAAGAA 
TTGCAAGGTG ACTTGGTTGA ATTAAGTAAA GCCAAATATT CCAGGAATAT CGTTAAGAAA 
TTTCTCATGT ATGGAAGTAA ACCACAGATT GCGGAGATAA TCAGAAGTTT TAAAGGTCAT 
GTGAGGAAGA TGCTGCGGCA TTCAGAGGCA TCGGCCATTG TGGAGTATGC GTACAACGAC 
AAAGCCATTT TGGAACAGAG GAACATGCTG ACAGAGGAGC TCTATGGGAA CACATTTCAG 
CTTTACAAGT CAGCAGATCA CCCAACGCTG GAAAAGGTGC TAGAGGTGCA GCCAGGGAAG 
CTAGAACTTA TCCTGGATGA GATGAAGCAG ATTCTGACTC CCATGGCCCA AAAGGAAGCT 
GTGATTAAGC ACTCACTGGT TCATAAAGTA TTCTTGGACT TTTTTACCTA TGCACCCCCA 
AAACTTCGAT CAGAACTAAT TGAAGCCATC CGGGAAGCAG TGGTGTACCT GGCCCACACA 
CACGATGGCG CCAGAGTGGC CATGCATTGC TTGTGGCATG GTACACCCAA GGACAGGAAA 
GTGATTGTGA AAACAATGAA GACGTATGTG GAGAAGATTG CTAATGGCCA GTACTCTCAC 
CTGGTTCTCC TGGCAGCGTT CGATTGTATT GATGATACCA AGCTTGTGAA GCAGATAATC 
ATATCAGAAG TCATCAGCTC CTTGCCCAGC ATCGTTAATG ACAAATATGG AAGGAAAGTG 
CTGCTGTACC TGCTGAGCCC CCGAGCCCCC GCCCACTTGG TGCCAGAGAT CATCCAGCTG 
CTGCAGAAGG GCGACGGCAA TGCACACAGT AAGAAGGACA CTGCAATCCG CCGGCGTGAG 
CTCTTGGAGT CCATCTCTCC AGCTTTGCTG AGCTACCTGC AAGGACACAC TCGGGAGGTG 
GTGCTGGACA AGTCCGTGTG TGTGCTGGTG TCTGACATCC TGGGATCTGC TACTGGGGAC 
GCTCAGCCTG CTATGGATGC CATCGCCGGT TTGGCAGCAG AGGAACTGTA TCCTGGGGGC 
AAGGACGGAG AGCTCCACAT TGCTGAGCAT CCTGCAGGAC ATCTGGTCCT CAAATGGTTA 
ATAGAGCAAG ATAAAAAGAT AAAAGAAAAT GGAAAAGAAG GTTGTTTTGC AAAAACCCTT 
GTAGAGTGCG TTGGTATGAA GAACCTCAAG TCCTGGGCCA GCATCAACCG AGGAGCCATC 
GTTCTTTCCA GCCTTCTTCA GAGTTGTGAC CAGGACGTTG TAAACAAAGT CAAAGCTGGA 
CTGAAAACCC TGATTCCTAC CTTGGAAAAA ACCAAAAGCA CCAGCAAAGG CATCCAGACT 
CTGCTTGAGA AGTTGACTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231308.1
CDS	97..2040
Translation	MEVKGKKKITGKSPQTSQGKNKFHKNSESSSAKAFPRKAAKEGGPKVTSKNFEKGATKPGKKRVKQFKNKPQGGKGPKDKFQKANKFNKKRKFQPDGKSDESAAKKPKWDDFKKKKKELKQNRQLSDKTNYDVVVRAKHIWESLRRKDCDKEKRVKLMSDLQKLIQGKIKTIAFAHDSTRVIQCLIQYGSEEQRKWAFEELQGDLVELSKAKYSRNIVKKFLMYGSKPQIAEIIRSFKGHVRKMLRHSEASAIVEYAYNDKAILEQRNMLTEELYGNTFQLYKSADHPTLEKVLEVQPGKLELILDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKLRSELIEAIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKIANGQYSHLVLLAAFDCIDDTKLVKQIIISEVISSLPSIVNDKYGRKVLLYLLSPRAPAHLVPEIIQLLQKGDGNAHSKKDTAIRRRELLESISPALLSYLQGHTREVVLDKSVCVLVSDILGSATGDAQPAMDAIAGLAAEELYPGGKDGELHIAEHPAGHLVLKWLIEQDKKIKENGKEGCFAKTLVECVGMKNLKSWASINRGAIVLSSLLQSCDQDVVNKVKAGLKTLIPTLEKTKSTSKGIQTLLEKLTA

Target ORF information:

RefSeq Version	XM_006231246.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pumilio RNA-binding family member 3 (Pum3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231246.3

ORF Insert Sequence:

ATGGAAGTCA AAGGGAAGAA AAAAATTACA GGAAAGAGTC CACAGACATC ACAAGGAAAG 
AATAAATTTC ATAAAAACAG TGAGTCTAGT TCTGCAAAGG CATTCCCAAG GAAAGCTGCT 
AAGGAAGGCG GGCCTAAAGT CACATCTAAG AACTTTGAGA AAGGTGCCAC AAAACCTGGC 
AAGAAGCGTG TGAAGCAGTT CAAGAACAAG CCACAAGGGG GCAAAGGACC GAAGGACAAA 
TTCCAGAAGG CAAATAAATT CAACAAGAAG CGGAAATTCC AGCCAGATGG GAAAAGTGAT 
GAATCAGCAG CCAAGAAACC CAAGTGGGAT GACTTCAAAA AGAAGAAGAA AGAGCTGAAG 
CAGAACAGAC AGCTCAGTGA TAAGACCAAT TACGACGTCG TGGTTCGAGC AAAGCACATT 
TGGGAGAGCT TGAGAAGAAA AGACTGTGAC AAAGAAAAAA GGGTGAAGCT CATGAGTGAT 
TTGCAGAAGC TGATTCAAGG AAAAATTAAA ACTATTGCCT TTGCGCATGA CTCAACTCGA 
GTGATCCAGT GCCTCATTCA GTATGGGAGC GAGGAGCAGA GGAAGTGGGC TTTCGAAGAA 
TTGCAAGGTG ACTTGGTTGA ATTAAGTAAA GCCAAATATT CCAGGAATAT CGTTAAGAAA 
TTTCTCATGT ATGGAAGTAA ACCACAGATT GCGGAGATAA TCAGAAGTTT TAAAGGTCAT 
GTGAGGAAGA TGCTGCGGCA TTCAGAGGCA TCGGCCATTG TGGAGTATGC GTACAACGAC 
AAAGCCATTT TGGAACAGAG GAACATGCTG ACAGAGGAGC TCTATGGGAA CACATTTCAG 
CTTTACAAGT CAGCAGATCA CCCAACGCTG GAAAAGGTGC TAGAGGTGCA GCCAGGGAAG 
CTAGAACTTA TCCTGGATGA GATGAAGCAG ATTCTGACTC CCATGGCCCA AAAGGAAGCT 
GTGATTAAGC ACTCACTGGT TCATAAAGTA TTCTTGGACT TTTTTACCTA TGCACCCCCA 
AAACTTCGAT CAGAACTAAT TGAAGCCATC CGGGAAGCAG TGGTGTACCT GGCCCACACA 
CACGATGGCG CCAGAGTGGC CATGCATTGC TTGTGGCATG GTACACCCAA GGACAGGAAA 
GTGATTGTGA AAACAATGAA GACGTATGTG GAGAAGATTG CTAATGGCCA GTACTCTCAC 
CTGGTTCTCC TGGCAGCGTT CGATTGTATT GATGATACCA AGCTTGTGAA GCAGATAATC 
ATATCAGAAG TCATCAGCTC CTTGCCCAGC ATCGTTAATG ACAAATATGG AAGGAAAGTG 
CTGCTGTACC TGCTGAGCCC CCGAGCCCCC GCCCACTTGG TGCCAGAGAT CATCCAGCTG 
CTGCAGAAGG GCGACGGCAA TGCACACAGT AAGAAGGACA CTGCAATCCG CCGGCGTGAG 
CTCTTGGAGT CCATCTCTCC AGCTTTGCTG AGCTACCTGC AAGGACACAC TCGGGAGGTG 
GTGCTGGACA AGTCCGTGTG TGTGCTGGTG TCTGACATCC TGGGATCTGC TACTGGGGAC 
GCTCAGCCTG CTATGGATGC CATCGCCGGT TTGGCAGCAG AGGAACTGTA TCCTGGGGGC 
AAGGACGGAG AGCTCCACAT TGCTGAGCAT CCTGCAGGAC ATCTGGTCCT CAAATGGTTA 
ATAGAGCAAG ATAAAAAGAT AAAAGAAAAT GGAAAAGAAG GTTGTTTTGC AAAAACCCTT 
GTAGAGTGCG TTGGTATGAA GAACCTCAAG TCCTGGGCCA GCATCAACCG AGGAGCCATC 
GTTCTTTCCA GCCTTCTTCA GAGTTGTGAC CAGGACGTTG TAAACAAAGT CAAAGCTGGA 
CTGAAAACCC TGATTCCTAC CTTGGAAAAA ACCAAAAGCA CCAGCAAAGG CATCCAGACT 
CTGCTTGAGA AGTTGACTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

hPuf-A/KIAA0020 modulates PARP-1 cleavage upon genotoxic stress.
Cancer research71(3)1126-34(2011 Feb)
Chang HY,Fan CC,Chu PC,Hong BE,Lee HJ,Chang MS

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Cell142(5)810-21(2010 Sep)
Ohta S,Bukowski-Wills JC,Sanchez-Pulido L,Alves Fde L,Wood L,Chen ZA,Platani M,Fischer L,Hudson DF,Ponting CP,Fukagawa T,Earnshaw WC,Rappsilber J

A visual intracellular classification strategy for uncharacterized human proteins.
Experimental cell research259(1)239-46(2000 Aug)
Hoja MR,Wahlestedt C,H??g C

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Pum3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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