The following Smcr8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smcr8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa08028 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001191919.1 | |
| Accession Version | NM_001191919.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-08 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | Smith-Magenis syndrome chromosomal region candidate gene 8 protein | |
| Comment | Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AABR07072125.1. On Jul 17, 2010 this sequence version replaced XM_573105.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGATCAGCG CCCCTGATGT GGTGGCCTTT ACCAAGGAAG ACGAATACGA GGAAGAACCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001178848.1 |
| CDS | 123..2930 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001191919.1 |
1 | ATGATCAGCG CCCCTGATGT GGTGGCCTTT ACCAAGGAAG ACGAATACGA GGAAGAACCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. |
C9orf72 binds SMCR8, localizes to lysosomes, and regulates mTORC1 signaling. |
A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy. |
Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death. |
The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway. |
