Gucy1a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gucy1a3
Entrez Gene ID	497757
Full Name	guanylate cyclase 1 soluble subunit alpha 3
Synonyms	Gucy1a1,SGC
General protein information	Preferred Names guanylate cyclase 1 soluble subunit alpha 3 Names guanylate cyclase soluble subunit alpha-3 GCS-alpha-1 GCS-alpha-3 Guanylate cyclase, soluble, alpha 1 (GTP pyrophosphate - lyase) guanylate cyclase 1, soluble, alpha 3 soluble guanylate cyclase large subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	soluble guanylate cyclase subunit that forms a heterodimer with Gucy1b3; may also function as an intracellular nitric oxide receptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017090.2	NP_058786.2	guanylate cyclase soluble subunit alpha-1
XM_008761107.2	XP_008759329.1	guanylate cyclase soluble subunit alpha-3 isoform X1
XM_017591011.1	XP_017446500.1	guanylate cyclase soluble subunit alpha-3 isoform X1
XM_006232518.3	XP_006232580.1	guanylate cyclase soluble subunit alpha-3 isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gucy1a3	XP_004911212.1
Canis lupus familiaris (dog)	GUCY1A3	NP_001018045.1
Bos taurus (cattle)	GUCY1A3	NP_786972.1
Homo sapiens (human)	GUCY1A3	NP_001124154.1
Mus musculus (house mouse)	Gucy1a3	NP_068696.2
Rattus norvegicus (Norway rat)	Gucy1a3	NP_058786.2
Pan troglodytes (chimpanzee)	GUCY1A3	XP_003310599.1
	GUCY1A3	XP_002804280.1
Gallus gallus (chicken)	GUCY1A3	XP_420375.1
Danio rerio (zebrafish)	gucy1a3	NP_001017725.1

Gene Ontology
Bibliography

Related articles in PubMed

Nitric oxide/cGMP signaling via guanylyl cyclase isoform 1 modulates glutamate and GABA release in somatosensory cortex of mice.
Wang Q, Mergia E, Koesling D, Mittmann T
Neuroscience360180-189(2017 Sep)

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
Sang?esa G, Shaligram S, Akther F, Roglans N, Laguna JC, Rahimian R, Alegret M
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)

The vasodilatory effect of sulfur dioxide via SGC/cGMP/PKG pathway in association with sulfhydryl-dependent dimerization.
Yao Q, Huang Y, Liu AD, Zhu M, Liu J, Yan H, Zhang Q, Geng B, Gao Y, Du S, Huang P, Tang C, Du J, Jin H
American journal of physiology. Regulatory, integrative and comparative physiology310(11)R1073-80(2016 Jun)

Alteration of basilar artery rho-kinase and soluble guanylyl cyclase protein expression in a rat model of cerebral vasospasm following subarachnoid hemorrhage.
Wang CJ, Lee PY, Wu BN, Wu SC, Loh JK, Tsai HP, Chung CL, Kassell NF, Kwan AL
BioMed research international2014531508(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hence, SO2 exerted its vasodilatory effects at least partly by promoting disulfide-dependent dimerization of sGC and PKG, resulting in an activated sGC/cGMP/PKG pathway in blood vessels. These findings revealed a new mode of action and mechanisms by which SO2 regulated the vascular tone.
Title: The vasodilatory effect of sulfur dioxide via SGC/cGMP/PKG pathway in association with sulfhydryl-dependent dimerization.

rhoA/rho-kinase and sGC/cGMP/PKG pathways play pivotal roles in cerebral vasospasm after subarachnoid hemorrhage.
Title: Alteration of basilar artery rho-kinase and soluble guanylyl cyclase protein expression in a rat model of cerebral vasospasm following subarachnoid hemorrhage.

sGC undergoes a reductive nitrosylation reaction that is coupled to the S-nitrosation of sGC cysteines.
Title: Heme-assisted S-nitrosation desensitizes ferric soluble guanylate cyclase to nitric oxide.

The effect of glucagon-like peptide-2 on the expression of the alpha1, alpha2, and beta1 subunits of soluble guanylyl cyclase, was studied.
Title: Glucagon-like peptide-2 (GLP-2) modulates the cGMP signalling pathway by regulating the expression of the soluble guanylyl cyclase receptor subunits in cultured rat astrocytes.

Mechanisms of sGC activation are investigated by electronic absorption and resonance Raman spectroscopy, confirming that stoichiometrically bound 1-nitric oxide (1-NO) contains five-coordinate NO-heme cofactor.
Title: Soluble guanylate cyclase is activated differently by excess NO and by YC-1: resonance Raman spectroscopic evidence.

The following Gucy1a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gucy1a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02789	NM_017090.2 Latest version!	Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 1 (Gucy1a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa02789	XM_008761107.2 Latest version!	Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 3 (Gucy1a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa02789	XM_006232518.3 Latest version!	Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 3 (Gucy1a3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa02789	XM_017591011.1 Latest version!	Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 3 (Gucy1a3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gucy1a3 ( NM_017090.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058786.2 Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 1 (Gucy1a1), mRNA.

Price & Availability↑

CloneID	ORa02789
Clone ID Related Accession (Same CDS sequence)	NM_017090.2 , XM_008761107.2 , XM_017591011.1 , XM_006232518.3
Accession Version	NM_017090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit alpha-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085746.1. On Jun 10, 2007 this sequence version replaced NM_017090.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085746.1, U60835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTTCTGCA GGAAGTTCAA AGATCTCAAG ATCACGGGGG AGTGTCCTTT CTCCTTACTG 
GCTCCTGGTC AGGTTCCTAC GGAGCCAATA GAGGAGGTGG CCGGAGTCTC TGAGAGCTGC 
CAGGCCACTC TGCCCACCTG CCAGGAGTTT GCTGAGAATG CAGAAGGGAG TCACCCCCAA 
AGAAAGACAA GCCGCAACAG AGTCTACCTA CACACCCTGG CAGAGAGTAT TGGCAAGCTC 
ATTTTCCCAG AGTTTGAGAG ACTGAACCTT GCACTTCAGA GAACATTGGC AAAGCACAAA 
ATAAAGGAAA ACAGGAATTC TTCAGAAAAA GAAGACCTTG AAAGAATAAT TGCAGAAGAA 
GCAATTGCGG CAGGTGTCCC AGTGGAGGCG CTCAAAGACT CTCTGGGTGA GGAACTGTTC 
AAGATCTGTT ATGAGGAAGA TGAGCACATC CTGGGCGTGG TTGGCGGCAC CCTGAAGGAC 
TTTCTCAACA GCTTCAGCAC GCTCCTGAAG CAGAGCAGCC ACTGCCAGGA GGCGGAGAGG 
CGGGGGCGAC TGGAAGATGC CTCCATCTTG TGCCTGGACA AGGACCAGGA CTTTCTAAAT 
GTTTACTACT TCTTCCCGAA GAGAACCACA GCCCTGCTTC TCCCCGGTAT CATTAAAGCG 
GCTGCTCGCA TACTGTACGA AAGCCACGTG GAGGTGTCCC TGATGCCTCC CTGCTTCCGA 
AGTGAGTGTA CCGAATTCGT GAACCAGCCC TATTTGCTCT ACTCGGTCCA CGTGAAGAGC 
ACCAAGCCTT CTCTGTCCCC AGGCAAACCC CAGTCCTCGC TAGTGATCCC TACTTCGCTC 
TTCTGCAAAA CTTTCCCATT CCACTTCATG CTGGACCGTG ACCTGGCCAT TCTGCAGCTG 
GGTAATGGCA TCAGAAGGCT GGTGAACAAG AGGGACTTCC AAGGGAAGCC CAACTTTGAA 
GAGTTCTTTG AAATTCTAAC TCCCAAAATC AACCAGACAT TTAGCGGCAT CATGACAATG 
TTGAATATGC AGTTTGTCAT ACGGGTGAGG AGATGGGATA ACTTAGTGAA GAAATCTTCA 
AGGGTTATGG ACCTCAAAGG TCAAATGATC TACATCGTTG AATCCAGTGC CATCTTGTTC 
TTAGGGTCGC CGTGTGTGGA CAGATTGGAA GATTTCACAG GACGGGGTCT CTATCTGTCT 
GACATCCCGA TTCATAATGC CCTGAGGGAT GTTGTCTTGA TAGGGGAACA GGCACGGGCT 
CAAGATGGCC TCAAGAAGAG GTTGGGAAAG CTGAAGGCAA CCTTGGAGCA TGCCCACCAA 
GCCCTGGAGG AGGAGAAGAA GAAGACGGTA GATCTCCTGT GTTCTATCTT TCCCTCTGAG 
GTTGCTCAGC AGCTCTGGCA AGGACAAATT GTGCAAGCCA AGAAGTTCAA TGAGGTCACC 
ATGCTCTTCT CAGATATCGT AGGGTTCACT GCCATCTGCT CTCAGTGTTC ACCTCTGCAG 
GTCATCACCA TGCTCAACGC TCTCTATACT CGCTTTGACC AACAGTGTGG AGAGCTGGAT 
GTCTACAAGG TGGAGACCAT CGGAGATGCA TATTGTGTGG CAGGGGGATT GCACAGAGAG 
AGTGACACAC ATGCTGTCCA GATAGCACTG ATGGCCCTGA AGATGATGGA GCTCTCCAAT 
GAGGTCATGT CTCCCCACGG AGAACCTATC AAGATGCGAA TCGGACTGCA TTCTGGGTCA 
GTGTTTGCTG GAGTTGTTGG AGTGAAGATG CCCCGATATT GCCTGTTTGG AAACAATGTC 
ACTCTGGCTA ACAAATTTGA GTCCTGCAGT GTGCCTCGGA AAATCAATGT CAGCCCCACG 
ACATACAGGT TACTCAAAGA CTGTCCTGGC TTTGTGTTCA CCCCGAGATC GAGGGAGGAG 
CTTCCACCAA ACTTCCCTAG TGACATTCCT GGAATCTGTC ACTTTCTGGA TGCTTATCAA 
CATCAAGGAC CTAATTCTAA ACCATGGTTC CAGCAGAAAG ATGCAGAAGA TGGAAACGCT 
AACTTCTTAG GCAAAGCATC AGGGGTAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058786.2
CDS	138..2210
Translation	MFCRKFKDLKITGECPFSLLAPGQVPTEPIEEVAGVSESCQATLPTCQEFAENAEGSHPQRKTSRNRVYLHTLAESIGKLIFPEFERLNLALQRTLAKHKIKENRNSSEKEDLERIIAEEAIAAGVPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSECTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPTSLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNLVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKKTVDLLCSIFPSEVAQQLWQGQIVQAKKFNEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYQHQGPNSKPWFQQKDAEDGNANFLGKASGVD

Target ORF information:

RefSeq Version	NM_017090.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 1 (Gucy1a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017090.2

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA AGATCTCAAG ATCACGGGGG AGTGTCCTTT CTCCTTACTG 
GCTCCTGGTC AGGTTCCTAC GGAGCCAATA GAGGAGGTGG CCGGAGTCTC TGAGAGCTGC 
CAGGCCACTC TGCCCACCTG CCAGGAGTTT GCTGAGAATG CAGAAGGGAG TCACCCCCAA 
AGAAAGACAA GCCGCAACAG AGTCTACCTA CACACCCTGG CAGAGAGTAT TGGCAAGCTC 
ATTTTCCCAG AGTTTGAGAG ACTGAACCTT GCACTTCAGA GAACATTGGC AAAGCACAAA 
ATAAAGGAAA ACAGGAATTC TTCAGAAAAA GAAGACCTTG AAAGAATAAT TGCAGAAGAA 
GCAATTGCGG CAGGTGTCCC AGTGGAGGCG CTCAAAGACT CTCTGGGTGA GGAACTGTTC 
AAGATCTGTT ATGAGGAAGA TGAGCACATC CTGGGCGTGG TTGGCGGCAC CCTGAAGGAC 
TTTCTCAACA GCTTCAGCAC GCTCCTGAAG CAGAGCAGCC ACTGCCAGGA GGCGGAGAGG 
CGGGGGCGAC TGGAAGATGC CTCCATCTTG TGCCTGGACA AGGACCAGGA CTTTCTAAAT 
GTTTACTACT TCTTCCCGAA GAGAACCACA GCCCTGCTTC TCCCCGGTAT CATTAAAGCG 
GCTGCTCGCA TACTGTACGA AAGCCACGTG GAGGTGTCCC TGATGCCTCC CTGCTTCCGA 
AGTGAGTGTA CCGAATTCGT GAACCAGCCC TATTTGCTCT ACTCGGTCCA CGTGAAGAGC 
ACCAAGCCTT CTCTGTCCCC AGGCAAACCC CAGTCCTCGC TAGTGATCCC TACTTCGCTC 
TTCTGCAAAA CTTTCCCATT CCACTTCATG CTGGACCGTG ACCTGGCCAT TCTGCAGCTG 
GGTAATGGCA TCAGAAGGCT GGTGAACAAG AGGGACTTCC AAGGGAAGCC CAACTTTGAA 
GAGTTCTTTG AAATTCTAAC TCCCAAAATC AACCAGACAT TTAGCGGCAT CATGACAATG 
TTGAATATGC AGTTTGTCAT ACGGGTGAGG AGATGGGATA ACTTAGTGAA GAAATCTTCA 
AGGGTTATGG ACCTCAAAGG TCAAATGATC TACATCGTTG AATCCAGTGC CATCTTGTTC 
TTAGGGTCGC CGTGTGTGGA CAGATTGGAA GATTTCACAG GACGGGGTCT CTATCTGTCT 
GACATCCCGA TTCATAATGC CCTGAGGGAT GTTGTCTTGA TAGGGGAACA GGCACGGGCT 
CAAGATGGCC TCAAGAAGAG GTTGGGAAAG CTGAAGGCAA CCTTGGAGCA TGCCCACCAA 
GCCCTGGAGG AGGAGAAGAA GAAGACGGTA GATCTCCTGT GTTCTATCTT TCCCTCTGAG 
GTTGCTCAGC AGCTCTGGCA AGGACAAATT GTGCAAGCCA AGAAGTTCAA TGAGGTCACC 
ATGCTCTTCT CAGATATCGT AGGGTTCACT GCCATCTGCT CTCAGTGTTC ACCTCTGCAG 
GTCATCACCA TGCTCAACGC TCTCTATACT CGCTTTGACC AACAGTGTGG AGAGCTGGAT 
GTCTACAAGG TGGAGACCAT CGGAGATGCA TATTGTGTGG CAGGGGGATT GCACAGAGAG 
AGTGACACAC ATGCTGTCCA GATAGCACTG ATGGCCCTGA AGATGATGGA GCTCTCCAAT 
GAGGTCATGT CTCCCCACGG AGAACCTATC AAGATGCGAA TCGGACTGCA TTCTGGGTCA 
GTGTTTGCTG GAGTTGTTGG AGTGAAGATG CCCCGATATT GCCTGTTTGG AAACAATGTC 
ACTCTGGCTA ACAAATTTGA GTCCTGCAGT GTGCCTCGGA AAATCAATGT CAGCCCCACG 
ACATACAGGT TACTCAAAGA CTGTCCTGGC TTTGTGTTCA CCCCGAGATC GAGGGAGGAG 
CTTCCACCAA ACTTCCCTAG TGACATTCCT GGAATCTGTC ACTTTCTGGA TGCTTATCAA 
CATCAAGGAC CTAATTCTAA ACCATGGTTC CAGCAGAAAG ATGCAGAAGA TGGAAACGCT 
AACTTCTTAG GCAAAGCATC AGGGGTAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1a3 ( XM_008761107.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759329.1 Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 3 (Gucy1a3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02789
Clone ID Related Accession (Same CDS sequence)	NM_017090.2 , XM_008761107.2 , XM_017591011.1 , XM_006232518.3
Accession Version	XM_008761107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCA GGAAGTTCAA AGATCTCAAG ATCACGGGGG AGTGTCCTTT CTCCTTACTG 
GCTCCTGGTC AGGTTCCTAC GGAGCCAATA GAGGAGGTGG CCGGAGTCTC TGAGAGCTGC 
CAGGCCACTC TGCCCACCTG CCAGGAGTTT GCTGAGAATG CAGAAGGGAG TCACCCCCAA 
AGAAAGACAA GCCGCAACAG AGTCTACCTA CACACCCTGG CAGAGAGTAT TGGCAAGCTC 
ATTTTCCCAG AGTTTGAGAG ACTGAACCTT GCACTTCAGA GAACATTGGC AAAGCACAAA 
ATAAAGGAAA ACAGGAATTC TTCAGAAAAA GAAGACCTTG AAAGAATAAT TGCAGAAGAA 
GCAATTGCGG CAGGTGTCCC AGTGGAGGCG CTCAAAGACT CTCTGGGTGA GGAACTGTTC 
AAGATCTGTT ATGAGGAAGA TGAGCACATC CTGGGCGTGG TTGGCGGCAC CCTGAAGGAC 
TTTCTCAACA GCTTCAGCAC GCTCCTGAAG CAGAGCAGCC ACTGCCAGGA GGCGGAGAGG 
CGGGGGCGAC TGGAAGATGC CTCCATCTTG TGCCTGGACA AGGACCAGGA CTTTCTAAAT 
GTTTACTACT TCTTCCCGAA GAGAACCACA GCCCTGCTTC TCCCCGGTAT CATTAAAGCG 
GCTGCTCGCA TACTGTACGA AAGCCACGTG GAGGTGTCCC TGATGCCTCC CTGCTTCCGA 
AGTGAGTGTA CCGAATTCGT GAACCAGCCC TATTTGCTCT ACTCGGTCCA CGTGAAGAGC 
ACCAAGCCTT CTCTGTCCCC AGGCAAACCC CAGTCCTCGC TAGTGATCCC TACTTCGCTC 
TTCTGCAAAA CTTTCCCATT CCACTTCATG CTGGACCGTG ACCTGGCCAT TCTGCAGCTG 
GGTAATGGCA TCAGAAGGCT GGTGAACAAG AGGGACTTCC AAGGGAAGCC CAACTTTGAA 
GAGTTCTTTG AAATTCTAAC TCCCAAAATC AACCAGACAT TTAGCGGCAT CATGACAATG 
TTGAATATGC AGTTTGTCAT ACGGGTGAGG AGATGGGATA ACTTAGTGAA GAAATCTTCA 
AGGGTTATGG ACCTCAAAGG TCAAATGATC TACATCGTTG AATCCAGTGC CATCTTGTTC 
TTAGGGTCGC CGTGTGTGGA CAGATTGGAA GATTTCACAG GACGGGGTCT CTATCTGTCT 
GACATCCCGA TTCATAATGC CCTGAGGGAT GTTGTCTTGA TAGGGGAACA GGCACGGGCT 
CAAGATGGCC TCAAGAAGAG GTTGGGAAAG CTGAAGGCAA CCTTGGAGCA TGCCCACCAA 
GCCCTGGAGG AGGAGAAGAA GAAGACGGTA GATCTCCTGT GTTCTATCTT TCCCTCTGAG 
GTTGCTCAGC AGCTCTGGCA AGGACAAATT GTGCAAGCCA AGAAGTTCAA TGAGGTCACC 
ATGCTCTTCT CAGATATCGT AGGGTTCACT GCCATCTGCT CTCAGTGTTC ACCTCTGCAG 
GTCATCACCA TGCTCAACGC TCTCTATACT CGCTTTGACC AACAGTGTGG AGAGCTGGAT 
GTCTACAAGG TGGAGACCAT CGGAGATGCA TATTGTGTGG CAGGGGGATT GCACAGAGAG 
AGTGACACAC ATGCTGTCCA GATAGCACTG ATGGCCCTGA AGATGATGGA GCTCTCCAAT 
GAGGTCATGT CTCCCCACGG AGAACCTATC AAGATGCGAA TCGGACTGCA TTCTGGGTCA 
GTGTTTGCTG GAGTTGTTGG AGTGAAGATG CCCCGATATT GCCTGTTTGG AAACAATGTC 
ACTCTGGCTA ACAAATTTGA GTCCTGCAGT GTGCCTCGGA AAATCAATGT CAGCCCCACG 
ACATACAGGT TACTCAAAGA CTGTCCTGGC TTTGTGTTCA CCCCGAGATC GAGGGAGGAG 
CTTCCACCAA ACTTCCCTAG TGACATTCCT GGAATCTGTC ACTTTCTGGA TGCTTATCAA 
CATCAAGGAC CTAATTCTAA ACCATGGTTC CAGCAGAAAG ATGCAGAAGA TGGAAACGCT 
AACTTCTTAG GCAAAGCATC AGGGGTAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759329.1
CDS	758..2830
Translation	MFCRKFKDLKITGECPFSLLAPGQVPTEPIEEVAGVSESCQATLPTCQEFAENAEGSHPQRKTSRNRVYLHTLAESIGKLIFPEFERLNLALQRTLAKHKIKENRNSSEKEDLERIIAEEAIAAGVPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSECTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPTSLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNLVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKKTVDLLCSIFPSEVAQQLWQGQIVQAKKFNEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYQHQGPNSKPWFQQKDAEDGNANFLGKASGVD

Target ORF information:

RefSeq Version	XM_008761107.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 3 (Gucy1a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761107.2

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA AGATCTCAAG ATCACGGGGG AGTGTCCTTT CTCCTTACTG 
GCTCCTGGTC AGGTTCCTAC GGAGCCAATA GAGGAGGTGG CCGGAGTCTC TGAGAGCTGC 
CAGGCCACTC TGCCCACCTG CCAGGAGTTT GCTGAGAATG CAGAAGGGAG TCACCCCCAA 
AGAAAGACAA GCCGCAACAG AGTCTACCTA CACACCCTGG CAGAGAGTAT TGGCAAGCTC 
ATTTTCCCAG AGTTTGAGAG ACTGAACCTT GCACTTCAGA GAACATTGGC AAAGCACAAA 
ATAAAGGAAA ACAGGAATTC TTCAGAAAAA GAAGACCTTG AAAGAATAAT TGCAGAAGAA 
GCAATTGCGG CAGGTGTCCC AGTGGAGGCG CTCAAAGACT CTCTGGGTGA GGAACTGTTC 
AAGATCTGTT ATGAGGAAGA TGAGCACATC CTGGGCGTGG TTGGCGGCAC CCTGAAGGAC 
TTTCTCAACA GCTTCAGCAC GCTCCTGAAG CAGAGCAGCC ACTGCCAGGA GGCGGAGAGG 
CGGGGGCGAC TGGAAGATGC CTCCATCTTG TGCCTGGACA AGGACCAGGA CTTTCTAAAT 
GTTTACTACT TCTTCCCGAA GAGAACCACA GCCCTGCTTC TCCCCGGTAT CATTAAAGCG 
GCTGCTCGCA TACTGTACGA AAGCCACGTG GAGGTGTCCC TGATGCCTCC CTGCTTCCGA 
AGTGAGTGTA CCGAATTCGT GAACCAGCCC TATTTGCTCT ACTCGGTCCA CGTGAAGAGC 
ACCAAGCCTT CTCTGTCCCC AGGCAAACCC CAGTCCTCGC TAGTGATCCC TACTTCGCTC 
TTCTGCAAAA CTTTCCCATT CCACTTCATG CTGGACCGTG ACCTGGCCAT TCTGCAGCTG 
GGTAATGGCA TCAGAAGGCT GGTGAACAAG AGGGACTTCC AAGGGAAGCC CAACTTTGAA 
GAGTTCTTTG AAATTCTAAC TCCCAAAATC AACCAGACAT TTAGCGGCAT CATGACAATG 
TTGAATATGC AGTTTGTCAT ACGGGTGAGG AGATGGGATA ACTTAGTGAA GAAATCTTCA 
AGGGTTATGG ACCTCAAAGG TCAAATGATC TACATCGTTG AATCCAGTGC CATCTTGTTC 
TTAGGGTCGC CGTGTGTGGA CAGATTGGAA GATTTCACAG GACGGGGTCT CTATCTGTCT 
GACATCCCGA TTCATAATGC CCTGAGGGAT GTTGTCTTGA TAGGGGAACA GGCACGGGCT 
CAAGATGGCC TCAAGAAGAG GTTGGGAAAG CTGAAGGCAA CCTTGGAGCA TGCCCACCAA 
GCCCTGGAGG AGGAGAAGAA GAAGACGGTA GATCTCCTGT GTTCTATCTT TCCCTCTGAG 
GTTGCTCAGC AGCTCTGGCA AGGACAAATT GTGCAAGCCA AGAAGTTCAA TGAGGTCACC 
ATGCTCTTCT CAGATATCGT AGGGTTCACT GCCATCTGCT CTCAGTGTTC ACCTCTGCAG 
GTCATCACCA TGCTCAACGC TCTCTATACT CGCTTTGACC AACAGTGTGG AGAGCTGGAT 
GTCTACAAGG TGGAGACCAT CGGAGATGCA TATTGTGTGG CAGGGGGATT GCACAGAGAG 
AGTGACACAC ATGCTGTCCA GATAGCACTG ATGGCCCTGA AGATGATGGA GCTCTCCAAT 
GAGGTCATGT CTCCCCACGG AGAACCTATC AAGATGCGAA TCGGACTGCA TTCTGGGTCA 
GTGTTTGCTG GAGTTGTTGG AGTGAAGATG CCCCGATATT GCCTGTTTGG AAACAATGTC 
ACTCTGGCTA ACAAATTTGA GTCCTGCAGT GTGCCTCGGA AAATCAATGT CAGCCCCACG 
ACATACAGGT TACTCAAAGA CTGTCCTGGC TTTGTGTTCA CCCCGAGATC GAGGGAGGAG 
CTTCCACCAA ACTTCCCTAG TGACATTCCT GGAATCTGTC ACTTTCTGGA TGCTTATCAA 
CATCAAGGAC CTAATTCTAA ACCATGGTTC CAGCAGAAAG ATGCAGAAGA TGGAAACGCT 
AACTTCTTAG GCAAAGCATC AGGGGTAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1a3 ( XM_017591011.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446500.1 Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 3 (Gucy1a3), transcript variant X2, mRNA.

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CloneID	ORa02789
Clone ID Related Accession (Same CDS sequence)	NM_017090.2 , XM_008761107.2 , XM_017591011.1 , XM_006232518.3
Accession Version	XM_017591011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCA GGAAGTTCAA AGATCTCAAG ATCACGGGGG AGTGTCCTTT CTCCTTACTG 
GCTCCTGGTC AGGTTCCTAC GGAGCCAATA GAGGAGGTGG CCGGAGTCTC TGAGAGCTGC 
CAGGCCACTC TGCCCACCTG CCAGGAGTTT GCTGAGAATG CAGAAGGGAG TCACCCCCAA 
AGAAAGACAA GCCGCAACAG AGTCTACCTA CACACCCTGG CAGAGAGTAT TGGCAAGCTC 
ATTTTCCCAG AGTTTGAGAG ACTGAACCTT GCACTTCAGA GAACATTGGC AAAGCACAAA 
ATAAAGGAAA ACAGGAATTC TTCAGAAAAA GAAGACCTTG AAAGAATAAT TGCAGAAGAA 
GCAATTGCGG CAGGTGTCCC AGTGGAGGCG CTCAAAGACT CTCTGGGTGA GGAACTGTTC 
AAGATCTGTT ATGAGGAAGA TGAGCACATC CTGGGCGTGG TTGGCGGCAC CCTGAAGGAC 
TTTCTCAACA GCTTCAGCAC GCTCCTGAAG CAGAGCAGCC ACTGCCAGGA GGCGGAGAGG 
CGGGGGCGAC TGGAAGATGC CTCCATCTTG TGCCTGGACA AGGACCAGGA CTTTCTAAAT 
GTTTACTACT TCTTCCCGAA GAGAACCACA GCCCTGCTTC TCCCCGGTAT CATTAAAGCG 
GCTGCTCGCA TACTGTACGA AAGCCACGTG GAGGTGTCCC TGATGCCTCC CTGCTTCCGA 
AGTGAGTGTA CCGAATTCGT GAACCAGCCC TATTTGCTCT ACTCGGTCCA CGTGAAGAGC 
ACCAAGCCTT CTCTGTCCCC AGGCAAACCC CAGTCCTCGC TAGTGATCCC TACTTCGCTC 
TTCTGCAAAA CTTTCCCATT CCACTTCATG CTGGACCGTG ACCTGGCCAT TCTGCAGCTG 
GGTAATGGCA TCAGAAGGCT GGTGAACAAG AGGGACTTCC AAGGGAAGCC CAACTTTGAA 
GAGTTCTTTG AAATTCTAAC TCCCAAAATC AACCAGACAT TTAGCGGCAT CATGACAATG 
TTGAATATGC AGTTTGTCAT ACGGGTGAGG AGATGGGATA ACTTAGTGAA GAAATCTTCA 
AGGGTTATGG ACCTCAAAGG TCAAATGATC TACATCGTTG AATCCAGTGC CATCTTGTTC 
TTAGGGTCGC CGTGTGTGGA CAGATTGGAA GATTTCACAG GACGGGGTCT CTATCTGTCT 
GACATCCCGA TTCATAATGC CCTGAGGGAT GTTGTCTTGA TAGGGGAACA GGCACGGGCT 
CAAGATGGCC TCAAGAAGAG GTTGGGAAAG CTGAAGGCAA CCTTGGAGCA TGCCCACCAA 
GCCCTGGAGG AGGAGAAGAA GAAGACGGTA GATCTCCTGT GTTCTATCTT TCCCTCTGAG 
GTTGCTCAGC AGCTCTGGCA AGGACAAATT GTGCAAGCCA AGAAGTTCAA TGAGGTCACC 
ATGCTCTTCT CAGATATCGT AGGGTTCACT GCCATCTGCT CTCAGTGTTC ACCTCTGCAG 
GTCATCACCA TGCTCAACGC TCTCTATACT CGCTTTGACC AACAGTGTGG AGAGCTGGAT 
GTCTACAAGG TGGAGACCAT CGGAGATGCA TATTGTGTGG CAGGGGGATT GCACAGAGAG 
AGTGACACAC ATGCTGTCCA GATAGCACTG ATGGCCCTGA AGATGATGGA GCTCTCCAAT 
GAGGTCATGT CTCCCCACGG AGAACCTATC AAGATGCGAA TCGGACTGCA TTCTGGGTCA 
GTGTTTGCTG GAGTTGTTGG AGTGAAGATG CCCCGATATT GCCTGTTTGG AAACAATGTC 
ACTCTGGCTA ACAAATTTGA GTCCTGCAGT GTGCCTCGGA AAATCAATGT CAGCCCCACG 
ACATACAGGT TACTCAAAGA CTGTCCTGGC TTTGTGTTCA CCCCGAGATC GAGGGAGGAG 
CTTCCACCAA ACTTCCCTAG TGACATTCCT GGAATCTGTC ACTTTCTGGA TGCTTATCAA 
CATCAAGGAC CTAATTCTAA ACCATGGTTC CAGCAGAAAG ATGCAGAAGA TGGAAACGCT 
AACTTCTTAG GCAAAGCATC AGGGGTAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446500.1
CDS	1095..3167
Translation	MFCRKFKDLKITGECPFSLLAPGQVPTEPIEEVAGVSESCQATLPTCQEFAENAEGSHPQRKTSRNRVYLHTLAESIGKLIFPEFERLNLALQRTLAKHKIKENRNSSEKEDLERIIAEEAIAAGVPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSECTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPTSLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNLVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKKTVDLLCSIFPSEVAQQLWQGQIVQAKKFNEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYQHQGPNSKPWFQQKDAEDGNANFLGKASGVD

Target ORF information:

RefSeq Version	XM_017591011.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 3 (Gucy1a3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591011.1

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA AGATCTCAAG ATCACGGGGG AGTGTCCTTT CTCCTTACTG 
GCTCCTGGTC AGGTTCCTAC GGAGCCAATA GAGGAGGTGG CCGGAGTCTC TGAGAGCTGC 
CAGGCCACTC TGCCCACCTG CCAGGAGTTT GCTGAGAATG CAGAAGGGAG TCACCCCCAA 
AGAAAGACAA GCCGCAACAG AGTCTACCTA CACACCCTGG CAGAGAGTAT TGGCAAGCTC 
ATTTTCCCAG AGTTTGAGAG ACTGAACCTT GCACTTCAGA GAACATTGGC AAAGCACAAA 
ATAAAGGAAA ACAGGAATTC TTCAGAAAAA GAAGACCTTG AAAGAATAAT TGCAGAAGAA 
GCAATTGCGG CAGGTGTCCC AGTGGAGGCG CTCAAAGACT CTCTGGGTGA GGAACTGTTC 
AAGATCTGTT ATGAGGAAGA TGAGCACATC CTGGGCGTGG TTGGCGGCAC CCTGAAGGAC 
TTTCTCAACA GCTTCAGCAC GCTCCTGAAG CAGAGCAGCC ACTGCCAGGA GGCGGAGAGG 
CGGGGGCGAC TGGAAGATGC CTCCATCTTG TGCCTGGACA AGGACCAGGA CTTTCTAAAT 
GTTTACTACT TCTTCCCGAA GAGAACCACA GCCCTGCTTC TCCCCGGTAT CATTAAAGCG 
GCTGCTCGCA TACTGTACGA AAGCCACGTG GAGGTGTCCC TGATGCCTCC CTGCTTCCGA 
AGTGAGTGTA CCGAATTCGT GAACCAGCCC TATTTGCTCT ACTCGGTCCA CGTGAAGAGC 
ACCAAGCCTT CTCTGTCCCC AGGCAAACCC CAGTCCTCGC TAGTGATCCC TACTTCGCTC 
TTCTGCAAAA CTTTCCCATT CCACTTCATG CTGGACCGTG ACCTGGCCAT TCTGCAGCTG 
GGTAATGGCA TCAGAAGGCT GGTGAACAAG AGGGACTTCC AAGGGAAGCC CAACTTTGAA 
GAGTTCTTTG AAATTCTAAC TCCCAAAATC AACCAGACAT TTAGCGGCAT CATGACAATG 
TTGAATATGC AGTTTGTCAT ACGGGTGAGG AGATGGGATA ACTTAGTGAA GAAATCTTCA 
AGGGTTATGG ACCTCAAAGG TCAAATGATC TACATCGTTG AATCCAGTGC CATCTTGTTC 
TTAGGGTCGC CGTGTGTGGA CAGATTGGAA GATTTCACAG GACGGGGTCT CTATCTGTCT 
GACATCCCGA TTCATAATGC CCTGAGGGAT GTTGTCTTGA TAGGGGAACA GGCACGGGCT 
CAAGATGGCC TCAAGAAGAG GTTGGGAAAG CTGAAGGCAA CCTTGGAGCA TGCCCACCAA 
GCCCTGGAGG AGGAGAAGAA GAAGACGGTA GATCTCCTGT GTTCTATCTT TCCCTCTGAG 
GTTGCTCAGC AGCTCTGGCA AGGACAAATT GTGCAAGCCA AGAAGTTCAA TGAGGTCACC 
ATGCTCTTCT CAGATATCGT AGGGTTCACT GCCATCTGCT CTCAGTGTTC ACCTCTGCAG 
GTCATCACCA TGCTCAACGC TCTCTATACT CGCTTTGACC AACAGTGTGG AGAGCTGGAT 
GTCTACAAGG TGGAGACCAT CGGAGATGCA TATTGTGTGG CAGGGGGATT GCACAGAGAG 
AGTGACACAC ATGCTGTCCA GATAGCACTG ATGGCCCTGA AGATGATGGA GCTCTCCAAT 
GAGGTCATGT CTCCCCACGG AGAACCTATC AAGATGCGAA TCGGACTGCA TTCTGGGTCA 
GTGTTTGCTG GAGTTGTTGG AGTGAAGATG CCCCGATATT GCCTGTTTGG AAACAATGTC 
ACTCTGGCTA ACAAATTTGA GTCCTGCAGT GTGCCTCGGA AAATCAATGT CAGCCCCACG 
ACATACAGGT TACTCAAAGA CTGTCCTGGC TTTGTGTTCA CCCCGAGATC GAGGGAGGAG 
CTTCCACCAA ACTTCCCTAG TGACATTCCT GGAATCTGTC ACTTTCTGGA TGCTTATCAA 
CATCAAGGAC CTAATTCTAA ACCATGGTTC CAGCAGAAAG ATGCAGAAGA TGGAAACGCT 
AACTTCTTAG GCAAAGCATC AGGGGTAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy1a3 ( XM_006232518.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232580.1 Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 3 (Gucy1a3), transcript variant X3, mRNA.

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CloneID	ORa02789
Clone ID Related Accession (Same CDS sequence)	NM_017090.2 , XM_008761107.2 , XM_017591011.1 , XM_006232518.3
Accession Version	XM_006232518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanylate cyclase soluble subunit alpha-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCA GGAAGTTCAA AGATCTCAAG ATCACGGGGG AGTGTCCTTT CTCCTTACTG 
GCTCCTGGTC AGGTTCCTAC GGAGCCAATA GAGGAGGTGG CCGGAGTCTC TGAGAGCTGC 
CAGGCCACTC TGCCCACCTG CCAGGAGTTT GCTGAGAATG CAGAAGGGAG TCACCCCCAA 
AGAAAGACAA GCCGCAACAG AGTCTACCTA CACACCCTGG CAGAGAGTAT TGGCAAGCTC 
ATTTTCCCAG AGTTTGAGAG ACTGAACCTT GCACTTCAGA GAACATTGGC AAAGCACAAA 
ATAAAGGAAA ACAGGAATTC TTCAGAAAAA GAAGACCTTG AAAGAATAAT TGCAGAAGAA 
GCAATTGCGG CAGGTGTCCC AGTGGAGGCG CTCAAAGACT CTCTGGGTGA GGAACTGTTC 
AAGATCTGTT ATGAGGAAGA TGAGCACATC CTGGGCGTGG TTGGCGGCAC CCTGAAGGAC 
TTTCTCAACA GCTTCAGCAC GCTCCTGAAG CAGAGCAGCC ACTGCCAGGA GGCGGAGAGG 
CGGGGGCGAC TGGAAGATGC CTCCATCTTG TGCCTGGACA AGGACCAGGA CTTTCTAAAT 
GTTTACTACT TCTTCCCGAA GAGAACCACA GCCCTGCTTC TCCCCGGTAT CATTAAAGCG 
GCTGCTCGCA TACTGTACGA AAGCCACGTG GAGGTGTCCC TGATGCCTCC CTGCTTCCGA 
AGTGAGTGTA CCGAATTCGT GAACCAGCCC TATTTGCTCT ACTCGGTCCA CGTGAAGAGC 
ACCAAGCCTT CTCTGTCCCC AGGCAAACCC CAGTCCTCGC TAGTGATCCC TACTTCGCTC 
TTCTGCAAAA CTTTCCCATT CCACTTCATG CTGGACCGTG ACCTGGCCAT TCTGCAGCTG 
GGTAATGGCA TCAGAAGGCT GGTGAACAAG AGGGACTTCC AAGGGAAGCC CAACTTTGAA 
GAGTTCTTTG AAATTCTAAC TCCCAAAATC AACCAGACAT TTAGCGGCAT CATGACAATG 
TTGAATATGC AGTTTGTCAT ACGGGTGAGG AGATGGGATA ACTTAGTGAA GAAATCTTCA 
AGGGTTATGG ACCTCAAAGG TCAAATGATC TACATCGTTG AATCCAGTGC CATCTTGTTC 
TTAGGGTCGC CGTGTGTGGA CAGATTGGAA GATTTCACAG GACGGGGTCT CTATCTGTCT 
GACATCCCGA TTCATAATGC CCTGAGGGAT GTTGTCTTGA TAGGGGAACA GGCACGGGCT 
CAAGATGGCC TCAAGAAGAG GTTGGGAAAG CTGAAGGCAA CCTTGGAGCA TGCCCACCAA 
GCCCTGGAGG AGGAGAAGAA GAAGACGGTA GATCTCCTGT GTTCTATCTT TCCCTCTGAG 
GTTGCTCAGC AGCTCTGGCA AGGACAAATT GTGCAAGCCA AGAAGTTCAA TGAGGTCACC 
ATGCTCTTCT CAGATATCGT AGGGTTCACT GCCATCTGCT CTCAGTGTTC ACCTCTGCAG 
GTCATCACCA TGCTCAACGC TCTCTATACT CGCTTTGACC AACAGTGTGG AGAGCTGGAT 
GTCTACAAGG TGGAGACCAT CGGAGATGCA TATTGTGTGG CAGGGGGATT GCACAGAGAG 
AGTGACACAC ATGCTGTCCA GATAGCACTG ATGGCCCTGA AGATGATGGA GCTCTCCAAT 
GAGGTCATGT CTCCCCACGG AGAACCTATC AAGATGCGAA TCGGACTGCA TTCTGGGTCA 
GTGTTTGCTG GAGTTGTTGG AGTGAAGATG CCCCGATATT GCCTGTTTGG AAACAATGTC 
ACTCTGGCTA ACAAATTTGA GTCCTGCAGT GTGCCTCGGA AAATCAATGT CAGCCCCACG 
ACATACAGGT TACTCAAAGA CTGTCCTGGC TTTGTGTTCA CCCCGAGATC GAGGGAGGAG 
CTTCCACCAA ACTTCCCTAG TGACATTCCT GGAATCTGTC ACTTTCTGGA TGCTTATCAA 
CATCAAGGAC CTAATTCTAA ACCATGGTTC CAGCAGAAAG ATGCAGAAGA TGGAAACGCT 
AACTTCTTAG GCAAAGCATC AGGGGTAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232580.1
CDS	729..2801
Translation	MFCRKFKDLKITGECPFSLLAPGQVPTEPIEEVAGVSESCQATLPTCQEFAENAEGSHPQRKTSRNRVYLHTLAESIGKLIFPEFERLNLALQRTLAKHKIKENRNSSEKEDLERIIAEEAIAAGVPVEALKDSLGEELFKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGIIKAAARILYESHVEVSLMPPCFRSECTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPTSLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWDNLVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKKTVDLLCSIFPSEVAQQLWQGQIVQAKKFNEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYQHQGPNSKPWFQQKDAEDGNANFLGKASGVD

Target ORF information:

RefSeq Version	XM_006232518.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus guanylate cyclase 1 soluble subunit alpha 3 (Gucy1a3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232518.3

ORF Insert Sequence:

ATGTTCTGCA GGAAGTTCAA AGATCTCAAG ATCACGGGGG AGTGTCCTTT CTCCTTACTG 
GCTCCTGGTC AGGTTCCTAC GGAGCCAATA GAGGAGGTGG CCGGAGTCTC TGAGAGCTGC 
CAGGCCACTC TGCCCACCTG CCAGGAGTTT GCTGAGAATG CAGAAGGGAG TCACCCCCAA 
AGAAAGACAA GCCGCAACAG AGTCTACCTA CACACCCTGG CAGAGAGTAT TGGCAAGCTC 
ATTTTCCCAG AGTTTGAGAG ACTGAACCTT GCACTTCAGA GAACATTGGC AAAGCACAAA 
ATAAAGGAAA ACAGGAATTC TTCAGAAAAA GAAGACCTTG AAAGAATAAT TGCAGAAGAA 
GCAATTGCGG CAGGTGTCCC AGTGGAGGCG CTCAAAGACT CTCTGGGTGA GGAACTGTTC 
AAGATCTGTT ATGAGGAAGA TGAGCACATC CTGGGCGTGG TTGGCGGCAC CCTGAAGGAC 
TTTCTCAACA GCTTCAGCAC GCTCCTGAAG CAGAGCAGCC ACTGCCAGGA GGCGGAGAGG 
CGGGGGCGAC TGGAAGATGC CTCCATCTTG TGCCTGGACA AGGACCAGGA CTTTCTAAAT 
GTTTACTACT TCTTCCCGAA GAGAACCACA GCCCTGCTTC TCCCCGGTAT CATTAAAGCG 
GCTGCTCGCA TACTGTACGA AAGCCACGTG GAGGTGTCCC TGATGCCTCC CTGCTTCCGA 
AGTGAGTGTA CCGAATTCGT GAACCAGCCC TATTTGCTCT ACTCGGTCCA CGTGAAGAGC 
ACCAAGCCTT CTCTGTCCCC AGGCAAACCC CAGTCCTCGC TAGTGATCCC TACTTCGCTC 
TTCTGCAAAA CTTTCCCATT CCACTTCATG CTGGACCGTG ACCTGGCCAT TCTGCAGCTG 
GGTAATGGCA TCAGAAGGCT GGTGAACAAG AGGGACTTCC AAGGGAAGCC CAACTTTGAA 
GAGTTCTTTG AAATTCTAAC TCCCAAAATC AACCAGACAT TTAGCGGCAT CATGACAATG 
TTGAATATGC AGTTTGTCAT ACGGGTGAGG AGATGGGATA ACTTAGTGAA GAAATCTTCA 
AGGGTTATGG ACCTCAAAGG TCAAATGATC TACATCGTTG AATCCAGTGC CATCTTGTTC 
TTAGGGTCGC CGTGTGTGGA CAGATTGGAA GATTTCACAG GACGGGGTCT CTATCTGTCT 
GACATCCCGA TTCATAATGC CCTGAGGGAT GTTGTCTTGA TAGGGGAACA GGCACGGGCT 
CAAGATGGCC TCAAGAAGAG GTTGGGAAAG CTGAAGGCAA CCTTGGAGCA TGCCCACCAA 
GCCCTGGAGG AGGAGAAGAA GAAGACGGTA GATCTCCTGT GTTCTATCTT TCCCTCTGAG 
GTTGCTCAGC AGCTCTGGCA AGGACAAATT GTGCAAGCCA AGAAGTTCAA TGAGGTCACC 
ATGCTCTTCT CAGATATCGT AGGGTTCACT GCCATCTGCT CTCAGTGTTC ACCTCTGCAG 
GTCATCACCA TGCTCAACGC TCTCTATACT CGCTTTGACC AACAGTGTGG AGAGCTGGAT 
GTCTACAAGG TGGAGACCAT CGGAGATGCA TATTGTGTGG CAGGGGGATT GCACAGAGAG 
AGTGACACAC ATGCTGTCCA GATAGCACTG ATGGCCCTGA AGATGATGGA GCTCTCCAAT 
GAGGTCATGT CTCCCCACGG AGAACCTATC AAGATGCGAA TCGGACTGCA TTCTGGGTCA 
GTGTTTGCTG GAGTTGTTGG AGTGAAGATG CCCCGATATT GCCTGTTTGG AAACAATGTC 
ACTCTGGCTA ACAAATTTGA GTCCTGCAGT GTGCCTCGGA AAATCAATGT CAGCCCCACG 
ACATACAGGT TACTCAAAGA CTGTCCTGGC TTTGTGTTCA CCCCGAGATC GAGGGAGGAG 
CTTCCACCAA ACTTCCCTAG TGACATTCCT GGAATCTGTC ACTTTCTGGA TGCTTATCAA 
CATCAAGGAC CTAATTCTAA ACCATGGTTC CAGCAGAAAG ATGCAGAAGA TGGAAACGCT 
AACTTCTTAG GCAAAGCATC AGGGGTAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nitric oxide/cGMP signaling via guanylyl cyclase isoform 1 modulates glutamate and GABA release in somatosensory cortex of mice.
Neuroscience360180-189(2017 Sep)
Wang Q,Mergia E,Koesling D,Mittmann T

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)
Sang?esa G,Shaligram S,Akther F,Roglans N,Laguna JC,Rahimian R,Alegret M

The vasodilatory effect of sulfur dioxide via SGC/cGMP/PKG pathway in association with sulfhydryl-dependent dimerization.
American journal of physiology. Regulatory, integrative and comparative physiology310(11)R1073-80(2016 Jun)
Yao Q,Huang Y,Liu AD,Zhu M,Liu J,Yan H,Zhang Q,Geng B,Gao Y,Du S,Huang P,Tang C,Du J,Jin H

Alteration of basilar artery rho-kinase and soluble guanylyl cyclase protein expression in a rat model of cerebral vasospasm following subarachnoid hemorrhage.
BioMed research international2014531508(2014)
Wang CJ,Lee PY,Wu BN,Wu SC,Loh JK,Tsai HP,Chung CL,Kassell NF,Kwan AL

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Gucy1a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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