Plcz1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plcz1
Entrez Gene ID	497197
Full Name	phospholipase C, zeta 1
General protein information	Preferred Names phospholipase C, zeta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 PLC-zeta-1 phosphoinositide phospholipase C-zeta-1 plcz1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q44

mRNA and Protein(s)

mRNA	Protein	Name
XM_006237597.3	XP_006237659.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1
XM_006237600.3	XP_006237662.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2
XM_017592770.1	XP_017448259.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1
XM_017592771.1	XP_017448260.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2
NM_001012234.1	NP_001012234.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PLCZ1	XP_005637152.1
Homo sapiens (human)	PLCZ1	NP_149114.2
Mus musculus (house mouse)	Plcz1	NP_473407.2
Rattus norvegicus (Norway rat)	Plcz1	NP_001012234.1
Bos taurus (cattle)	PLCZ1	NP_001011680.2
Gallus gallus (chicken)	PLCZ1	NP_001034362.1
Pan troglodytes (chimpanzee)	PLCZ1	XP_001160159.1
	PLCZ1	XP_001095846.2

Gene Ontology
Bibliography

Related articles in PubMed

Male infertility-linked point mutation reveals a vital binding role for the C2 domain of sperm PLC?.
Nomikos M, Stamatiadis P, Sanders JR, Beck K, Calver BL, Buntwal L, Lofty M, Sideratou Z, Swann K, Lai FA
The Biochemical journal474(6)1003-1016(2017 03)

Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.
Escoffier J, Lee HC, Yassine S, Zouari R, Martinez G, Karaouz?ne T, Coutton C, Kherraf ZE, Halouani L, Triki C, Nef S, Thierry-Mieg N, Savinov SN, Fissore R, Ray PF, Arnoult C
Human molecular genetics25(5)878-91(2016 Mar)

Functional disparity between human PAWP and PLC? in the generation of Ca2+ oscillations for oocyte activation.
Nomikos M, Sanders JR, Kashir J, Sanusi R, Buntwal L, Love D, Ashley P, Sanders D, Knaggs P, Bunkheila A, Swann K, Lai FA
Molecular human reproduction21(9)702-10(2015 Sep)

Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish.
Ito M, Shikano T, Oda S, Horiguchi T, Tanimoto S, Awaji T, Mitani H, Miyazaki S
Biology of reproduction78(6)1081-90(2008 Jun)

Recombinant phospholipase Czeta has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggs.
Kouchi Z, Fukami K, Shikano T, Oda S, Nakamura Y, Takenawa T, Miyazaki S
The Journal of biological chemistry279(11)10408-12(2004 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

All PLCZ1 proteins including fish could induce Ca(2+) oscillations in mouse eggs, but the activity was variable in the order of human >> mouse > medaka >> rat, estimated from minimal RNA concentration to induce Ca(2+) spikes.
Title: Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish.

The following Plcz1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plcz1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02804	NM_001012234.1 Latest version!	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$377.30 $539.00
ORa27868	XM_017592770.1 Latest version!	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa27868	XM_006237597.3 Latest version!	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa27869	XM_017592771.1 Latest version!	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa27869	XM_006237600.3 Latest version!	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plcz1 ( NM_001012234.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012234.1 Rattus norvegicus phospholipase C, zeta 1 (Plcz1), mRNA.

Price & Availability↑

CloneID	ORa02804
Clone ID Related Accession (Same CDS sequence)	NM_001012234.1
Accession Version	NM_001012234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY885259.1. On Apr 20, 2005 this sequence version replaced XM_575711.1. ##Evidence-Data-START## Transcript exon combination :: AY885259.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGCC ATTATGAGCT CGCAGAAGCA AGATGGTTTA TGTCAAAGAT TCAGGATTAT 
TTTAGAGGTG GAAAAATCAG CGCTGGAATT ACTCACAAAC TGCTCGAGAA ACTTGATTTC 
CCATGCCATT TTGCTCATGT GAAACGTATT TTTAAGGAAA ATGACAGACA TAACCAAGGA 
AGAATCACCA CCGAAGATTT TAGAACCATC TATCGGTGTA TTGTACATAG AGAAGAGATC 
GTTGAGATTT TCAACACGTA TACTGAAAAC AGGAAAATTC TCCCCGAGGA CAGTCTGATT 
GAATTTCTAA CCCAAGAGCA GTATGAAATG GAGATGGATG AGTCCAGTTC AGTGGAGATC 
ATCCAGAAGT ACGAGCCCAT TGCAGAAGTA AAGAACGAGC GGCAGATGTC AATTGAAGGT 
TTTGCAAGAT ACATGTTTTC TTCAGAATGT CTACTGTTTA AAGAGACGTG TAACACAGTG 
TACCAAGATA TGAATAAGCC ACTAAATGAT TACTATATTT CATCGTCTCA CAACACATAT 
TTGATATCTG ATCAAATATT GGGACCAAGT GACATTTGGG GATATATAAG TGCCCTCGTG 
AAAGGCTGCC GCTGTCTGGA AATTGACTGC TGGGATGGAG CACAAAATGA ACCCATTGTG 
TACCATGGCT ACACTCTCAC CAGCAAGCTT CTCTTCAAAA CCGTTATCCA AGCAATAAAC 
AAGTACGCCT TCGTGACGTC TGATTACCCA GTGGTGCTGT CCTTAGAGAA TCACTGCTCC 
CCTGGTCAAC AGGAAGTGAT GACCGACATT CTGCAGAGTA CCTTTGGAGA CTTTCTGCTC 
TCAGACATAC TTGACGAGTT TCCAGACAGT TTGCCATCTC CAGAGGCACT AAAATTCAAA 
ATATTAGTGA AAAATAAGAA AGTTGGAACC TTATCTGAAA CCCGCGAGAG GCTGGGGACT 
GACAAAAGGG GCATAGCGCT AGACTTGGAA GAAGAAATCT ATGAAAATGA AGACGAAGAC 
TCAGGAAAGG AGCCAGAAAC GTGGGATGAT TTCCTGTCAC GGGTTAAGGA GGAGCAGGAG 
GCAGACCCCT CAACGTTGAG CGGAATAGCA GATGCCAAGA AAAAGATCAG GAAGCTAAGA 
GTAGCTCTGG CCTTATCTGA TCTTGTCATT TATACCAAAG CTGAGAAGTT CCGAAACTTC 
CAATATTCAA GAGTCTATCA GCAGTTTAAT GAGACCACTT CTATGGGAGA GTCTCGAGCT 
CGAAAACTTT CAAAATTGAG AGCCCATGAG TTTATTTTCC ACACTGCAGC GTTCATCACC 
AGAGTGTACC CCAAGTTCAC GAGAGCAGAC TCTTCTAATT TTAATCCTCA AGAGTTTTGG 
AATGTGGGCT GTCAGATGGT GGCCTTGAAT TTTCAAACCC CTGGACTGCC TATGGATTTG 
CAAAACGGGA AATTTTTGGA TAATGGAGGC TCTGGATATG TTTTGAAGCC AGACTTTCTT 
AGAGACACAA CTTTGGGCTT TAACCCAAAT GAACCAGAAG GAGATGGCCA TCCGGTTACC 
CTCACGATCC GACTCATCAG TGGGATCCAG TTGCCTGTTA ACGTGCCCTC AAATACATCT 
GACATAATAG TGATCATAGA AGTCTACGGT GTCCCAAACG ACCACATGAA GCAGCAGAGT 
CGTGCCGTTA AGAACAATGC TTTTAGTCCA AGGTGGAATG AAACATTCAC ATTTCTTATT 
CAAGTGCCAG AATTGGCACT GATACGTTTC GTTGTTGAAA CTCAAGGCTT CCTATCGGGA 
AATGAATTAC TTGGGCAGTA CACTTTACCC GTTCTTTGCA TGAACAAAGG TTATCGTCGC 
GTTCCTCTGT TTTCCAAATC CGGTGCGAAC CTTGAACCTT CCTCTCTGTT TATTTACGTT 
TGGTACTACA GAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012234.1
CDS	1..1938
Translation	MESHYELAEARWFMSKIQDYFRGGKISAGITHKLLEKLDFPCHFAHVKRIFKENDRHNQGRITTEDFRTIYRCIVHREEIVEIFNTYTENRKILPEDSLIEFLTQEQYEMEMDESSSVEIIQKYEPIAEVKNERQMSIEGFARYMFSSECLLFKETCNTVYQDMNKPLNDYYISSSHNTYLISDQILGPSDIWGYISALVKGCRCLEIDCWDGAQNEPIVYHGYTLTSKLLFKTVIQAINKYAFVTSDYPVVLSLENHCSPGQQEVMTDILQSTFGDFLLSDILDEFPDSLPSPEALKFKILVKNKKVGTLSETRERLGTDKRGIALDLEEEIYENEDEDSGKEPETWDDFLSRVKEEQEADPSTLSGIADAKKKIRKLRVALALSDLVIYTKAEKFRNFQYSRVYQQFNETTSMGESRARKLSKLRAHEFIFHTAAFITRVYPKFTRADSSNFNPQEFWNVGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYVLKPDFLRDTTLGFNPNEPEGDGHPVTLTIRLISGIQLPVNVPSNTSDIIVIIEVYGVPNDHMKQQSRAVKNNAFSPRWNETFTFLIQVPELALIRFVVETQGFLSGNELLGQYTLPVLCMNKGYRRVPLFSKSGANLEPSSLFIYVWYYRE

Target ORF information:

RefSeq Version	NM_001012234.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012234.1

ORF Insert Sequence:

ATGGAGAGCC ATTATGAGCT CGCAGAAGCA AGATGGTTTA TGTCAAAGAT TCAGGATTAT 
TTTAGAGGTG GAAAAATCAG CGCTGGAATT ACTCACAAAC TGCTCGAGAA ACTTGATTTC 
CCATGCCATT TTGCTCATGT GAAACGTATT TTTAAGGAAA ATGACAGACA TAACCAAGGA 
AGAATCACCA CCGAAGATTT TAGAACCATC TATCGGTGTA TTGTACATAG AGAAGAGATC 
GTTGAGATTT TCAACACGTA TACTGAAAAC AGGAAAATTC TCCCCGAGGA CAGTCTGATT 
GAATTTCTAA CCCAAGAGCA GTATGAAATG GAGATGGATG AGTCCAGTTC AGTGGAGATC 
ATCCAGAAGT ACGAGCCCAT TGCAGAAGTA AAGAACGAGC GGCAGATGTC AATTGAAGGT 
TTTGCAAGAT ACATGTTTTC TTCAGAATGT CTACTGTTTA AAGAGACGTG TAACACAGTG 
TACCAAGATA TGAATAAGCC ACTAAATGAT TACTATATTT CATCGTCTCA CAACACATAT 
TTGATATCTG ATCAAATATT GGGACCAAGT GACATTTGGG GATATATAAG TGCCCTCGTG 
AAAGGCTGCC GCTGTCTGGA AATTGACTGC TGGGATGGAG CACAAAATGA ACCCATTGTG 
TACCATGGCT ACACTCTCAC CAGCAAGCTT CTCTTCAAAA CCGTTATCCA AGCAATAAAC 
AAGTACGCCT TCGTGACGTC TGATTACCCA GTGGTGCTGT CCTTAGAGAA TCACTGCTCC 
CCTGGTCAAC AGGAAGTGAT GACCGACATT CTGCAGAGTA CCTTTGGAGA CTTTCTGCTC 
TCAGACATAC TTGACGAGTT TCCAGACAGT TTGCCATCTC CAGAGGCACT AAAATTCAAA 
ATATTAGTGA AAAATAAGAA AGTTGGAACC TTATCTGAAA CCCGCGAGAG GCTGGGGACT 
GACAAAAGGG GCATAGCGCT AGACTTGGAA GAAGAAATCT ATGAAAATGA AGACGAAGAC 
TCAGGAAAGG AGCCAGAAAC GTGGGATGAT TTCCTGTCAC GGGTTAAGGA GGAGCAGGAG 
GCAGACCCCT CAACGTTGAG CGGAATAGCA GATGCCAAGA AAAAGATCAG GAAGCTAAGA 
GTAGCTCTGG CCTTATCTGA TCTTGTCATT TATACCAAAG CTGAGAAGTT CCGAAACTTC 
CAATATTCAA GAGTCTATCA GCAGTTTAAT GAGACCACTT CTATGGGAGA GTCTCGAGCT 
CGAAAACTTT CAAAATTGAG AGCCCATGAG TTTATTTTCC ACACTGCAGC GTTCATCACC 
AGAGTGTACC CCAAGTTCAC GAGAGCAGAC TCTTCTAATT TTAATCCTCA AGAGTTTTGG 
AATGTGGGCT GTCAGATGGT GGCCTTGAAT TTTCAAACCC CTGGACTGCC TATGGATTTG 
CAAAACGGGA AATTTTTGGA TAATGGAGGC TCTGGATATG TTTTGAAGCC AGACTTTCTT 
AGAGACACAA CTTTGGGCTT TAACCCAAAT GAACCAGAAG GAGATGGCCA TCCGGTTACC 
CTCACGATCC GACTCATCAG TGGGATCCAG TTGCCTGTTA ACGTGCCCTC AAATACATCT 
GACATAATAG TGATCATAGA AGTCTACGGT GTCCCAAACG ACCACATGAA GCAGCAGAGT 
CGTGCCGTTA AGAACAATGC TTTTAGTCCA AGGTGGAATG AAACATTCAC ATTTCTTATT 
CAAGTGCCAG AATTGGCACT GATACGTTTC GTTGTTGAAA CTCAAGGCTT CCTATCGGGA 
AATGAATTAC TTGGGCAGTA CACTTTACCC GTTCTTTGCA TGAACAAAGG TTATCGTCGC 
GTTCCTCTGT TTTCCAAATC CGGTGCGAAC CTTGAACCTT CCTCTCTGTT TATTTACGTT 
TGGTACTACA GAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcz1 ( XM_006237597.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237659.1 Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27868
Clone ID Related Accession (Same CDS sequence)	XM_006237597.3 , XM_017592770.1
Accession Version	XM_006237597.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCC ATTATGAGCT CGCAGAAGCA AGATGGTTTA TGTCAAAGAT TCAGGATTAT 
TTTAGAGGTG GAAAAATCAG CGCTGGAATT ACTCACAAAC TGCTCGAGAA ACTTGATTTC 
CCATGCCATT TTGCTCATGT GAAACGTATT TTTAAGGAAA ATGACAGACA TAACCAAGGA 
AGAATCACCA CCGAAGATTT TAGAACCATC TATCGGTGTA TTGTACATAG AGAAGAGATC 
GTTGAGATTT TCAACACGTA TACTGAAAAC AGGAAAATTC TCCCCGAGGA CAGTCTGATT 
GAATTTCTAA CCCAAGAGCA GTATGAAATG GAGATGGATG AGTCCAGTTC AGTGGAGATC 
ATCCAGAAGT ACGAGCCCAT TGCAGAAGTA AAGAACGAGC GGCAGATGTC AATTGAAGGT 
TTTGCAAGAT ACATGTTTTC TTCAGAATGT CTACTGTTTA AAGAGACGTG TAACACAGTG 
TACCAAGATA TGAATAAGCC ACTAAATGAT TACTATATTT CATCGTCTCA CAACACATAT 
TTGATATCTG ATCAAATATT GGGACCAAGT GACATTTGGG GATATATAAG TGCCCTCGTG 
AAAGGCTGCC GCTGTCTGGA AATTGACTGC TGGGATGGAG CACAAAATGA ACCCATTGTG 
TACCATGGCT ACACTCTCAC CAGCAAGCTT CTCTTCAAAA CCGTTATCCA AGCAATAAAC 
AAGTACGCCT TCGTGACGTC TGATTACCCA GTGGTGCTGT CCTTAGAGAA TCACTGCTCC 
CCTGGTCAAC AGGAAGTGAT GACCGACATT CTGCAGAGTA CCTTTGGAGA CTTTCTGCTC 
TCAGACATAC TTGACGAGTT TCCAGACAGT TTGCCATCTC CAGAGGCACT GAAATTTAAA 
ATATTAGTGA AAAATAAGAA AGTTGGAACC TTATCTGAAA CCCGCGAGAG GCTGGGGACT 
GACAAAAGGG GCATAGCGCT AGACTTGGAA GAAGAAATCT ATGAAAATGA AGACGAAGAC 
TCAGGAAAGG AGCCAGAAAC GTGGGATGAT TTCCTGTCAC GGGTTAAGGA GGAGCAGGAG 
GCAGACCCCT CAACGTTGAG CGGAATAGCA GATGCCAAGA AAAAGATCAG GAAGCTAAGA 
GTAGCTCTGG CCTTATCTGA TCTTGTCATT TATACCAAAG CTGAGAAGTT CCGAAACTTC 
CAATATTCAA GAGTCTATCA GCAGTTTAAT GAGACCACTT CTATGGGAGA GTCTCGAGCT 
CGAAAACTTT CAAAATTGAG AGCCCATGAG TTTATTTTCC ACACTGCAGC GTTCATCACC 
AGAGTGTACC CCAAGTTCAC GAGAGCAGAC TCTTCTAATT TTAATCCTCA AGAGTTTTGG 
AATGTGGGCT GTCAGATGGT GGCCTTGAAT TTTCAAACCC CTGGACTGCC TATGGATTTG 
CAAAACGGGA AATTTTTGGA TAATGGAGGC TCTGGATATG TTTTGAAGCC AGACTTTCTT 
AGAGACACAA CTTTGGGCTT TAACCCAAAT GAACCAGAAG GAGATGGCCA TCCGGTTACC 
CTCACGATCC GACTCATCAG TGGGATCCAG TTGCCTGTTA ACGTGCCCTC AAATACATCT 
GACATAATAG TGATCATAGA AGTCTACGGT GTCCCAAACG ACCACATGAA GCAGCAGAGT 
CGTGCCGTTA AGAACAATGC TTTTAGTCCA AGGTGGAATG AAACATTCAC ATTTCTTATT 
CAAGTGCCAG AATTGGCACT GATACGTTTC GTTGTTGAAA CTCAAGGCTT CCTATCGGGA 
AATGAATTAC TTGGGCAGTA CACTTTACCC GTTCTTTGCA TGAACAAAGG TTATCGTCGC 
GTTCCTCTGT TTTCCAAATC CGGTGCGAAC CTTGAACCTT CCTCTCTGTT TATTTACGTT 
TGGTACTACA GAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237659.1
CDS	229..2166
Translation	MESHYELAEARWFMSKIQDYFRGGKISAGITHKLLEKLDFPCHFAHVKRIFKENDRHNQGRITTEDFRTIYRCIVHREEIVEIFNTYTENRKILPEDSLIEFLTQEQYEMEMDESSSVEIIQKYEPIAEVKNERQMSIEGFARYMFSSECLLFKETCNTVYQDMNKPLNDYYISSSHNTYLISDQILGPSDIWGYISALVKGCRCLEIDCWDGAQNEPIVYHGYTLTSKLLFKTVIQAINKYAFVTSDYPVVLSLENHCSPGQQEVMTDILQSTFGDFLLSDILDEFPDSLPSPEALKFKILVKNKKVGTLSETRERLGTDKRGIALDLEEEIYENEDEDSGKEPETWDDFLSRVKEEQEADPSTLSGIADAKKKIRKLRVALALSDLVIYTKAEKFRNFQYSRVYQQFNETTSMGESRARKLSKLRAHEFIFHTAAFITRVYPKFTRADSSNFNPQEFWNVGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYVLKPDFLRDTTLGFNPNEPEGDGHPVTLTIRLISGIQLPVNVPSNTSDIIVIIEVYGVPNDHMKQQSRAVKNNAFSPRWNETFTFLIQVPELALIRFVVETQGFLSGNELLGQYTLPVLCMNKGYRRVPLFSKSGANLEPSSLFIYVWYYRE

Target ORF information:

RefSeq Version	XM_006237597.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237597.3

ORF Insert Sequence:

ATGGAGAGCC ATTATGAGCT CGCAGAAGCA AGATGGTTTA TGTCAAAGAT TCAGGATTAT 
TTTAGAGGTG GAAAAATCAG CGCTGGAATT ACTCACAAAC TGCTCGAGAA ACTTGATTTC 
CCATGCCATT TTGCTCATGT GAAACGTATT TTTAAGGAAA ATGACAGACA TAACCAAGGA 
AGAATCACCA CCGAAGATTT TAGAACCATC TATCGGTGTA TTGTACATAG AGAAGAGATC 
GTTGAGATTT TCAACACGTA TACTGAAAAC AGGAAAATTC TCCCCGAGGA CAGTCTGATT 
GAATTTCTAA CCCAAGAGCA GTATGAAATG GAGATGGATG AGTCCAGTTC AGTGGAGATC 
ATCCAGAAGT ACGAGCCCAT TGCAGAAGTA AAGAACGAGC GGCAGATGTC AATTGAAGGT 
TTTGCAAGAT ACATGTTTTC TTCAGAATGT CTACTGTTTA AAGAGACGTG TAACACAGTG 
TACCAAGATA TGAATAAGCC ACTAAATGAT TACTATATTT CATCGTCTCA CAACACATAT 
TTGATATCTG ATCAAATATT GGGACCAAGT GACATTTGGG GATATATAAG TGCCCTCGTG 
AAAGGCTGCC GCTGTCTGGA AATTGACTGC TGGGATGGAG CACAAAATGA ACCCATTGTG 
TACCATGGCT ACACTCTCAC CAGCAAGCTT CTCTTCAAAA CCGTTATCCA AGCAATAAAC 
AAGTACGCCT TCGTGACGTC TGATTACCCA GTGGTGCTGT CCTTAGAGAA TCACTGCTCC 
CCTGGTCAAC AGGAAGTGAT GACCGACATT CTGCAGAGTA CCTTTGGAGA CTTTCTGCTC 
TCAGACATAC TTGACGAGTT TCCAGACAGT TTGCCATCTC CAGAGGCACT GAAATTTAAA 
ATATTAGTGA AAAATAAGAA AGTTGGAACC TTATCTGAAA CCCGCGAGAG GCTGGGGACT 
GACAAAAGGG GCATAGCGCT AGACTTGGAA GAAGAAATCT ATGAAAATGA AGACGAAGAC 
TCAGGAAAGG AGCCAGAAAC GTGGGATGAT TTCCTGTCAC GGGTTAAGGA GGAGCAGGAG 
GCAGACCCCT CAACGTTGAG CGGAATAGCA GATGCCAAGA AAAAGATCAG GAAGCTAAGA 
GTAGCTCTGG CCTTATCTGA TCTTGTCATT TATACCAAAG CTGAGAAGTT CCGAAACTTC 
CAATATTCAA GAGTCTATCA GCAGTTTAAT GAGACCACTT CTATGGGAGA GTCTCGAGCT 
CGAAAACTTT CAAAATTGAG AGCCCATGAG TTTATTTTCC ACACTGCAGC GTTCATCACC 
AGAGTGTACC CCAAGTTCAC GAGAGCAGAC TCTTCTAATT TTAATCCTCA AGAGTTTTGG 
AATGTGGGCT GTCAGATGGT GGCCTTGAAT TTTCAAACCC CTGGACTGCC TATGGATTTG 
CAAAACGGGA AATTTTTGGA TAATGGAGGC TCTGGATATG TTTTGAAGCC AGACTTTCTT 
AGAGACACAA CTTTGGGCTT TAACCCAAAT GAACCAGAAG GAGATGGCCA TCCGGTTACC 
CTCACGATCC GACTCATCAG TGGGATCCAG TTGCCTGTTA ACGTGCCCTC AAATACATCT 
GACATAATAG TGATCATAGA AGTCTACGGT GTCCCAAACG ACCACATGAA GCAGCAGAGT 
CGTGCCGTTA AGAACAATGC TTTTAGTCCA AGGTGGAATG AAACATTCAC ATTTCTTATT 
CAAGTGCCAG AATTGGCACT GATACGTTTC GTTGTTGAAA CTCAAGGCTT CCTATCGGGA 
AATGAATTAC TTGGGCAGTA CACTTTACCC GTTCTTTGCA TGAACAAAGG TTATCGTCGC 
GTTCCTCTGT TTTCCAAATC CGGTGCGAAC CTTGAACCTT CCTCTCTGTT TATTTACGTT 
TGGTACTACA GAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcz1 ( XM_017592770.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448259.1 Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa27868
Clone ID Related Accession (Same CDS sequence)	XM_006237597.3 , XM_017592770.1
Accession Version	XM_017592770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCC ATTATGAGCT CGCAGAAGCA AGATGGTTTA TGTCAAAGAT TCAGGATTAT 
TTTAGAGGTG GAAAAATCAG CGCTGGAATT ACTCACAAAC TGCTCGAGAA ACTTGATTTC 
CCATGCCATT TTGCTCATGT GAAACGTATT TTTAAGGAAA ATGACAGACA TAACCAAGGA 
AGAATCACCA CCGAAGATTT TAGAACCATC TATCGGTGTA TTGTACATAG AGAAGAGATC 
GTTGAGATTT TCAACACGTA TACTGAAAAC AGGAAAATTC TCCCCGAGGA CAGTCTGATT 
GAATTTCTAA CCCAAGAGCA GTATGAAATG GAGATGGATG AGTCCAGTTC AGTGGAGATC 
ATCCAGAAGT ACGAGCCCAT TGCAGAAGTA AAGAACGAGC GGCAGATGTC AATTGAAGGT 
TTTGCAAGAT ACATGTTTTC TTCAGAATGT CTACTGTTTA AAGAGACGTG TAACACAGTG 
TACCAAGATA TGAATAAGCC ACTAAATGAT TACTATATTT CATCGTCTCA CAACACATAT 
TTGATATCTG ATCAAATATT GGGACCAAGT GACATTTGGG GATATATAAG TGCCCTCGTG 
AAAGGCTGCC GCTGTCTGGA AATTGACTGC TGGGATGGAG CACAAAATGA ACCCATTGTG 
TACCATGGCT ACACTCTCAC CAGCAAGCTT CTCTTCAAAA CCGTTATCCA AGCAATAAAC 
AAGTACGCCT TCGTGACGTC TGATTACCCA GTGGTGCTGT CCTTAGAGAA TCACTGCTCC 
CCTGGTCAAC AGGAAGTGAT GACCGACATT CTGCAGAGTA CCTTTGGAGA CTTTCTGCTC 
TCAGACATAC TTGACGAGTT TCCAGACAGT TTGCCATCTC CAGAGGCACT GAAATTTAAA 
ATATTAGTGA AAAATAAGAA AGTTGGAACC TTATCTGAAA CCCGCGAGAG GCTGGGGACT 
GACAAAAGGG GCATAGCGCT AGACTTGGAA GAAGAAATCT ATGAAAATGA AGACGAAGAC 
TCAGGAAAGG AGCCAGAAAC GTGGGATGAT TTCCTGTCAC GGGTTAAGGA GGAGCAGGAG 
GCAGACCCCT CAACGTTGAG CGGAATAGCA GATGCCAAGA AAAAGATCAG GAAGCTAAGA 
GTAGCTCTGG CCTTATCTGA TCTTGTCATT TATACCAAAG CTGAGAAGTT CCGAAACTTC 
CAATATTCAA GAGTCTATCA GCAGTTTAAT GAGACCACTT CTATGGGAGA GTCTCGAGCT 
CGAAAACTTT CAAAATTGAG AGCCCATGAG TTTATTTTCC ACACTGCAGC GTTCATCACC 
AGAGTGTACC CCAAGTTCAC GAGAGCAGAC TCTTCTAATT TTAATCCTCA AGAGTTTTGG 
AATGTGGGCT GTCAGATGGT GGCCTTGAAT TTTCAAACCC CTGGACTGCC TATGGATTTG 
CAAAACGGGA AATTTTTGGA TAATGGAGGC TCTGGATATG TTTTGAAGCC AGACTTTCTT 
AGAGACACAA CTTTGGGCTT TAACCCAAAT GAACCAGAAG GAGATGGCCA TCCGGTTACC 
CTCACGATCC GACTCATCAG TGGGATCCAG TTGCCTGTTA ACGTGCCCTC AAATACATCT 
GACATAATAG TGATCATAGA AGTCTACGGT GTCCCAAACG ACCACATGAA GCAGCAGAGT 
CGTGCCGTTA AGAACAATGC TTTTAGTCCA AGGTGGAATG AAACATTCAC ATTTCTTATT 
CAAGTGCCAG AATTGGCACT GATACGTTTC GTTGTTGAAA CTCAAGGCTT CCTATCGGGA 
AATGAATTAC TTGGGCAGTA CACTTTACCC GTTCTTTGCA TGAACAAAGG TTATCGTCGC 
GTTCCTCTGT TTTCCAAATC CGGTGCGAAC CTTGAACCTT CCTCTCTGTT TATTTACGTT 
TGGTACTACA GAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448259.1
CDS	222..2159
Translation	MESHYELAEARWFMSKIQDYFRGGKISAGITHKLLEKLDFPCHFAHVKRIFKENDRHNQGRITTEDFRTIYRCIVHREEIVEIFNTYTENRKILPEDSLIEFLTQEQYEMEMDESSSVEIIQKYEPIAEVKNERQMSIEGFARYMFSSECLLFKETCNTVYQDMNKPLNDYYISSSHNTYLISDQILGPSDIWGYISALVKGCRCLEIDCWDGAQNEPIVYHGYTLTSKLLFKTVIQAINKYAFVTSDYPVVLSLENHCSPGQQEVMTDILQSTFGDFLLSDILDEFPDSLPSPEALKFKILVKNKKVGTLSETRERLGTDKRGIALDLEEEIYENEDEDSGKEPETWDDFLSRVKEEQEADPSTLSGIADAKKKIRKLRVALALSDLVIYTKAEKFRNFQYSRVYQQFNETTSMGESRARKLSKLRAHEFIFHTAAFITRVYPKFTRADSSNFNPQEFWNVGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYVLKPDFLRDTTLGFNPNEPEGDGHPVTLTIRLISGIQLPVNVPSNTSDIIVIIEVYGVPNDHMKQQSRAVKNNAFSPRWNETFTFLIQVPELALIRFVVETQGFLSGNELLGQYTLPVLCMNKGYRRVPLFSKSGANLEPSSLFIYVWYYRE

Target ORF information:

RefSeq Version	XM_017592770.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592770.1

ORF Insert Sequence:

ATGGAGAGCC ATTATGAGCT CGCAGAAGCA AGATGGTTTA TGTCAAAGAT TCAGGATTAT 
TTTAGAGGTG GAAAAATCAG CGCTGGAATT ACTCACAAAC TGCTCGAGAA ACTTGATTTC 
CCATGCCATT TTGCTCATGT GAAACGTATT TTTAAGGAAA ATGACAGACA TAACCAAGGA 
AGAATCACCA CCGAAGATTT TAGAACCATC TATCGGTGTA TTGTACATAG AGAAGAGATC 
GTTGAGATTT TCAACACGTA TACTGAAAAC AGGAAAATTC TCCCCGAGGA CAGTCTGATT 
GAATTTCTAA CCCAAGAGCA GTATGAAATG GAGATGGATG AGTCCAGTTC AGTGGAGATC 
ATCCAGAAGT ACGAGCCCAT TGCAGAAGTA AAGAACGAGC GGCAGATGTC AATTGAAGGT 
TTTGCAAGAT ACATGTTTTC TTCAGAATGT CTACTGTTTA AAGAGACGTG TAACACAGTG 
TACCAAGATA TGAATAAGCC ACTAAATGAT TACTATATTT CATCGTCTCA CAACACATAT 
TTGATATCTG ATCAAATATT GGGACCAAGT GACATTTGGG GATATATAAG TGCCCTCGTG 
AAAGGCTGCC GCTGTCTGGA AATTGACTGC TGGGATGGAG CACAAAATGA ACCCATTGTG 
TACCATGGCT ACACTCTCAC CAGCAAGCTT CTCTTCAAAA CCGTTATCCA AGCAATAAAC 
AAGTACGCCT TCGTGACGTC TGATTACCCA GTGGTGCTGT CCTTAGAGAA TCACTGCTCC 
CCTGGTCAAC AGGAAGTGAT GACCGACATT CTGCAGAGTA CCTTTGGAGA CTTTCTGCTC 
TCAGACATAC TTGACGAGTT TCCAGACAGT TTGCCATCTC CAGAGGCACT GAAATTTAAA 
ATATTAGTGA AAAATAAGAA AGTTGGAACC TTATCTGAAA CCCGCGAGAG GCTGGGGACT 
GACAAAAGGG GCATAGCGCT AGACTTGGAA GAAGAAATCT ATGAAAATGA AGACGAAGAC 
TCAGGAAAGG AGCCAGAAAC GTGGGATGAT TTCCTGTCAC GGGTTAAGGA GGAGCAGGAG 
GCAGACCCCT CAACGTTGAG CGGAATAGCA GATGCCAAGA AAAAGATCAG GAAGCTAAGA 
GTAGCTCTGG CCTTATCTGA TCTTGTCATT TATACCAAAG CTGAGAAGTT CCGAAACTTC 
CAATATTCAA GAGTCTATCA GCAGTTTAAT GAGACCACTT CTATGGGAGA GTCTCGAGCT 
CGAAAACTTT CAAAATTGAG AGCCCATGAG TTTATTTTCC ACACTGCAGC GTTCATCACC 
AGAGTGTACC CCAAGTTCAC GAGAGCAGAC TCTTCTAATT TTAATCCTCA AGAGTTTTGG 
AATGTGGGCT GTCAGATGGT GGCCTTGAAT TTTCAAACCC CTGGACTGCC TATGGATTTG 
CAAAACGGGA AATTTTTGGA TAATGGAGGC TCTGGATATG TTTTGAAGCC AGACTTTCTT 
AGAGACACAA CTTTGGGCTT TAACCCAAAT GAACCAGAAG GAGATGGCCA TCCGGTTACC 
CTCACGATCC GACTCATCAG TGGGATCCAG TTGCCTGTTA ACGTGCCCTC AAATACATCT 
GACATAATAG TGATCATAGA AGTCTACGGT GTCCCAAACG ACCACATGAA GCAGCAGAGT 
CGTGCCGTTA AGAACAATGC TTTTAGTCCA AGGTGGAATG AAACATTCAC ATTTCTTATT 
CAAGTGCCAG AATTGGCACT GATACGTTTC GTTGTTGAAA CTCAAGGCTT CCTATCGGGA 
AATGAATTAC TTGGGCAGTA CACTTTACCC GTTCTTTGCA TGAACAAAGG TTATCGTCGC 
GTTCCTCTGT TTTCCAAATC CGGTGCGAAC CTTGAACCTT CCTCTCTGTT TATTTACGTT 
TGGTACTACA GAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcz1 ( XM_006237600.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237662.1 Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa27869
Clone ID Related Accession (Same CDS sequence)	XM_006237600.3 , XM_017592771.1
Accession Version	XM_006237600.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237600.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTGTG AACACAGGAT CATGGCCTGC CTGGTCCAGT TTATGGAAGG AACTGAAGTG 
GAATGGACCA CACAAAGGAA ATACGAAAAT GACAGACATA ACCAAGGAAG AATCACCACC 
GAAGATTTTA GAACCATCTA TCGGTGTATT GTACATAGAG AAGAGATCGT TGAGATTTTC 
AACACGTATA CTGAAAACAG GAAAATTCTC CCCGAGGACA GTCTGATTGA ATTTCTAACC 
CAAGAGCAGT ATGAAATGGA GATGGATGAG TCCAGTTCAG TGGAGATCAT CCAGAAGTAC 
GAGCCCATTG CAGAAGTAAA GAACGAGCGG CAGATGTCAA TTGAAGGTTT TGCAAGATAC 
ATGTTTTCTT CAGAATGTCT ACTGTTTAAA GAGACGTGTA ACACAGTGTA CCAAGATATG 
AATAAGCCAC TAAATGATTA CTATATTTCA TCGTCTCACA ACACATATTT GATATCTGAT 
CAAATATTGG GACCAAGTGA CATTTGGGGA TATATAAGTG CCCTCGTGAA AGGCTGCCGC 
TGTCTGGAAA TTGACTGCTG GGATGGAGCA CAAAATGAAC CCATTGTGTA CCATGGCTAC 
ACTCTCACCA GCAAGCTTCT CTTCAAAACC GTTATCCAAG CAATAAACAA GTACGCCTTC 
GTGACGTCTG ATTACCCAGT GGTGCTGTCC TTAGAGAATC ACTGCTCCCC TGGTCAACAG 
GAAGTGATGA CCGACATTCT GCAGAGTACC TTTGGAGACT TTCTGCTCTC AGACATACTT 
GACGAGTTTC CAGACAGTTT GCCATCTCCA GAGGCACTGA AATTTAAAAT ATTAGTGAAA 
AATAAGAAAG TTGGAACCTT ATCTGAAACC CGCGAGAGGC TGGGGACTGA CAAAAGGGGC 
ATAGCGCTAG ACTTGGAAGA AGAAATCTAT GAAAATGAAG ACGAAGACTC AGGAAAGGAG 
CCAGAAACGT GGGATGATTT CCTGTCACGG GTTAAGGAGG AGCAGGAGGC AGACCCCTCA 
ACGTTGAGCG GAATAGCAGA TGCCAAGAAA AAGATCAGGA AGCTAAGAGT AGCTCTGGCC 
TTATCTGATC TTGTCATTTA TACCAAAGCT GAGAAGTTCC GAAACTTCCA ATATTCAAGA 
GTCTATCAGC AGTTTAATGA GACCACTTCT ATGGGAGAGT CTCGAGCTCG AAAACTTTCA 
AAATTGAGAG CCCATGAGTT TATTTTCCAC ACTGCAGCGT TCATCACCAG AGTGTACCCC 
AAGTTCACGA GAGCAGACTC TTCTAATTTT AATCCTCAAG AGTTTTGGAA TGTGGGCTGT 
CAGATGGTGG CCTTGAATTT TCAAACCCCT GGACTGCCTA TGGATTTGCA AAACGGGAAA 
TTTTTGGATA ATGGAGGCTC TGGATATGTT TTGAAGCCAG ACTTTCTTAG AGACACAACT 
TTGGGCTTTA ACCCAAATGA ACCAGAAGGA GATGGCCATC CGGTTACCCT CACGATCCGA 
CTCATCAGTG GGATCCAGTT GCCTGTTAAC GTGCCCTCAA ATACATCTGA CATAATAGTG 
ATCATAGAAG TCTACGGTGT CCCAAACGAC CACATGAAGC AGCAGAGTCG TGCCGTTAAG 
AACAATGCTT TTAGTCCAAG GTGGAATGAA ACATTCACAT TTCTTATTCA AGTGCCAGAA 
TTGGCACTGA TACGTTTCGT TGTTGAAACT CAAGGCTTCC TATCGGGAAA TGAATTACTT 
GGGCAGTACA CTTTACCCGT TCTTTGCATG AACAAAGGTT ATCGTCGCGT TCCTCTGTTT 
TCCAAATCCG GTGCGAACCT TGAACCTTCC TCTCTGTTTA TTTACGTTTG GTACTACAGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237662.1
CDS	172..2037
Translation	MNCEHRIMACLVQFMEGTEVEWTTQRKYENDRHNQGRITTEDFRTIYRCIVHREEIVEIFNTYTENRKILPEDSLIEFLTQEQYEMEMDESSSVEIIQKYEPIAEVKNERQMSIEGFARYMFSSECLLFKETCNTVYQDMNKPLNDYYISSSHNTYLISDQILGPSDIWGYISALVKGCRCLEIDCWDGAQNEPIVYHGYTLTSKLLFKTVIQAINKYAFVTSDYPVVLSLENHCSPGQQEVMTDILQSTFGDFLLSDILDEFPDSLPSPEALKFKILVKNKKVGTLSETRERLGTDKRGIALDLEEEIYENEDEDSGKEPETWDDFLSRVKEEQEADPSTLSGIADAKKKIRKLRVALALSDLVIYTKAEKFRNFQYSRVYQQFNETTSMGESRARKLSKLRAHEFIFHTAAFITRVYPKFTRADSSNFNPQEFWNVGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYVLKPDFLRDTTLGFNPNEPEGDGHPVTLTIRLISGIQLPVNVPSNTSDIIVIIEVYGVPNDHMKQQSRAVKNNAFSPRWNETFTFLIQVPELALIRFVVETQGFLSGNELLGQYTLPVLCMNKGYRRVPLFSKSGANLEPSSLFIYVWYYRE

Target ORF information:

RefSeq Version	XM_006237600.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237600.3

ORF Insert Sequence:

ATGAATTGTG AACACAGGAT CATGGCCTGC CTGGTCCAGT TTATGGAAGG AACTGAAGTG 
GAATGGACCA CACAAAGGAA ATACGAAAAT GACAGACATA ACCAAGGAAG AATCACCACC 
GAAGATTTTA GAACCATCTA TCGGTGTATT GTACATAGAG AAGAGATCGT TGAGATTTTC 
AACACGTATA CTGAAAACAG GAAAATTCTC CCCGAGGACA GTCTGATTGA ATTTCTAACC 
CAAGAGCAGT ATGAAATGGA GATGGATGAG TCCAGTTCAG TGGAGATCAT CCAGAAGTAC 
GAGCCCATTG CAGAAGTAAA GAACGAGCGG CAGATGTCAA TTGAAGGTTT TGCAAGATAC 
ATGTTTTCTT CAGAATGTCT ACTGTTTAAA GAGACGTGTA ACACAGTGTA CCAAGATATG 
AATAAGCCAC TAAATGATTA CTATATTTCA TCGTCTCACA ACACATATTT GATATCTGAT 
CAAATATTGG GACCAAGTGA CATTTGGGGA TATATAAGTG CCCTCGTGAA AGGCTGCCGC 
TGTCTGGAAA TTGACTGCTG GGATGGAGCA CAAAATGAAC CCATTGTGTA CCATGGCTAC 
ACTCTCACCA GCAAGCTTCT CTTCAAAACC GTTATCCAAG CAATAAACAA GTACGCCTTC 
GTGACGTCTG ATTACCCAGT GGTGCTGTCC TTAGAGAATC ACTGCTCCCC TGGTCAACAG 
GAAGTGATGA CCGACATTCT GCAGAGTACC TTTGGAGACT TTCTGCTCTC AGACATACTT 
GACGAGTTTC CAGACAGTTT GCCATCTCCA GAGGCACTGA AATTTAAAAT ATTAGTGAAA 
AATAAGAAAG TTGGAACCTT ATCTGAAACC CGCGAGAGGC TGGGGACTGA CAAAAGGGGC 
ATAGCGCTAG ACTTGGAAGA AGAAATCTAT GAAAATGAAG ACGAAGACTC AGGAAAGGAG 
CCAGAAACGT GGGATGATTT CCTGTCACGG GTTAAGGAGG AGCAGGAGGC AGACCCCTCA 
ACGTTGAGCG GAATAGCAGA TGCCAAGAAA AAGATCAGGA AGCTAAGAGT AGCTCTGGCC 
TTATCTGATC TTGTCATTTA TACCAAAGCT GAGAAGTTCC GAAACTTCCA ATATTCAAGA 
GTCTATCAGC AGTTTAATGA GACCACTTCT ATGGGAGAGT CTCGAGCTCG AAAACTTTCA 
AAATTGAGAG CCCATGAGTT TATTTTCCAC ACTGCAGCGT TCATCACCAG AGTGTACCCC 
AAGTTCACGA GAGCAGACTC TTCTAATTTT AATCCTCAAG AGTTTTGGAA TGTGGGCTGT 
CAGATGGTGG CCTTGAATTT TCAAACCCCT GGACTGCCTA TGGATTTGCA AAACGGGAAA 
TTTTTGGATA ATGGAGGCTC TGGATATGTT TTGAAGCCAG ACTTTCTTAG AGACACAACT 
TTGGGCTTTA ACCCAAATGA ACCAGAAGGA GATGGCCATC CGGTTACCCT CACGATCCGA 
CTCATCAGTG GGATCCAGTT GCCTGTTAAC GTGCCCTCAA ATACATCTGA CATAATAGTG 
ATCATAGAAG TCTACGGTGT CCCAAACGAC CACATGAAGC AGCAGAGTCG TGCCGTTAAG 
AACAATGCTT TTAGTCCAAG GTGGAATGAA ACATTCACAT TTCTTATTCA AGTGCCAGAA 
TTGGCACTGA TACGTTTCGT TGTTGAAACT CAAGGCTTCC TATCGGGAAA TGAATTACTT 
GGGCAGTACA CTTTACCCGT TCTTTGCATG AACAAAGGTT ATCGTCGCGT TCCTCTGTTT 
TCCAAATCCG GTGCGAACCT TGAACCTTCC TCTCTGTTTA TTTACGTTTG GTACTACAGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcz1 ( XM_017592771.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448260.1 Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa27869
Clone ID Related Accession (Same CDS sequence)	XM_006237600.3 , XM_017592771.1
Accession Version	XM_017592771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTGTG AACACAGGAT CATGGCCTGC CTGGTCCAGT TTATGGAAGG AACTGAAGTG 
GAATGGACCA CACAAAGGAA ATACGAAAAT GACAGACATA ACCAAGGAAG AATCACCACC 
GAAGATTTTA GAACCATCTA TCGGTGTATT GTACATAGAG AAGAGATCGT TGAGATTTTC 
AACACGTATA CTGAAAACAG GAAAATTCTC CCCGAGGACA GTCTGATTGA ATTTCTAACC 
CAAGAGCAGT ATGAAATGGA GATGGATGAG TCCAGTTCAG TGGAGATCAT CCAGAAGTAC 
GAGCCCATTG CAGAAGTAAA GAACGAGCGG CAGATGTCAA TTGAAGGTTT TGCAAGATAC 
ATGTTTTCTT CAGAATGTCT ACTGTTTAAA GAGACGTGTA ACACAGTGTA CCAAGATATG 
AATAAGCCAC TAAATGATTA CTATATTTCA TCGTCTCACA ACACATATTT GATATCTGAT 
CAAATATTGG GACCAAGTGA CATTTGGGGA TATATAAGTG CCCTCGTGAA AGGCTGCCGC 
TGTCTGGAAA TTGACTGCTG GGATGGAGCA CAAAATGAAC CCATTGTGTA CCATGGCTAC 
ACTCTCACCA GCAAGCTTCT CTTCAAAACC GTTATCCAAG CAATAAACAA GTACGCCTTC 
GTGACGTCTG ATTACCCAGT GGTGCTGTCC TTAGAGAATC ACTGCTCCCC TGGTCAACAG 
GAAGTGATGA CCGACATTCT GCAGAGTACC TTTGGAGACT TTCTGCTCTC AGACATACTT 
GACGAGTTTC CAGACAGTTT GCCATCTCCA GAGGCACTGA AATTTAAAAT ATTAGTGAAA 
AATAAGAAAG TTGGAACCTT ATCTGAAACC CGCGAGAGGC TGGGGACTGA CAAAAGGGGC 
ATAGCGCTAG ACTTGGAAGA AGAAATCTAT GAAAATGAAG ACGAAGACTC AGGAAAGGAG 
CCAGAAACGT GGGATGATTT CCTGTCACGG GTTAAGGAGG AGCAGGAGGC AGACCCCTCA 
ACGTTGAGCG GAATAGCAGA TGCCAAGAAA AAGATCAGGA AGCTAAGAGT AGCTCTGGCC 
TTATCTGATC TTGTCATTTA TACCAAAGCT GAGAAGTTCC GAAACTTCCA ATATTCAAGA 
GTCTATCAGC AGTTTAATGA GACCACTTCT ATGGGAGAGT CTCGAGCTCG AAAACTTTCA 
AAATTGAGAG CCCATGAGTT TATTTTCCAC ACTGCAGCGT TCATCACCAG AGTGTACCCC 
AAGTTCACGA GAGCAGACTC TTCTAATTTT AATCCTCAAG AGTTTTGGAA TGTGGGCTGT 
CAGATGGTGG CCTTGAATTT TCAAACCCCT GGACTGCCTA TGGATTTGCA AAACGGGAAA 
TTTTTGGATA ATGGAGGCTC TGGATATGTT TTGAAGCCAG ACTTTCTTAG AGACACAACT 
TTGGGCTTTA ACCCAAATGA ACCAGAAGGA GATGGCCATC CGGTTACCCT CACGATCCGA 
CTCATCAGTG GGATCCAGTT GCCTGTTAAC GTGCCCTCAA ATACATCTGA CATAATAGTG 
ATCATAGAAG TCTACGGTGT CCCAAACGAC CACATGAAGC AGCAGAGTCG TGCCGTTAAG 
AACAATGCTT TTAGTCCAAG GTGGAATGAA ACATTCACAT TTCTTATTCA AGTGCCAGAA 
TTGGCACTGA TACGTTTCGT TGTTGAAACT CAAGGCTTCC TATCGGGAAA TGAATTACTT 
GGGCAGTACA CTTTACCCGT TCTTTGCATG AACAAAGGTT ATCGTCGCGT TCCTCTGTTT 
TCCAAATCCG GTGCGAACCT TGAACCTTCC TCTCTGTTTA TTTACGTTTG GTACTACAGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448260.1
CDS	159..2024
Translation	MNCEHRIMACLVQFMEGTEVEWTTQRKYENDRHNQGRITTEDFRTIYRCIVHREEIVEIFNTYTENRKILPEDSLIEFLTQEQYEMEMDESSSVEIIQKYEPIAEVKNERQMSIEGFARYMFSSECLLFKETCNTVYQDMNKPLNDYYISSSHNTYLISDQILGPSDIWGYISALVKGCRCLEIDCWDGAQNEPIVYHGYTLTSKLLFKTVIQAINKYAFVTSDYPVVLSLENHCSPGQQEVMTDILQSTFGDFLLSDILDEFPDSLPSPEALKFKILVKNKKVGTLSETRERLGTDKRGIALDLEEEIYENEDEDSGKEPETWDDFLSRVKEEQEADPSTLSGIADAKKKIRKLRVALALSDLVIYTKAEKFRNFQYSRVYQQFNETTSMGESRARKLSKLRAHEFIFHTAAFITRVYPKFTRADSSNFNPQEFWNVGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYVLKPDFLRDTTLGFNPNEPEGDGHPVTLTIRLISGIQLPVNVPSNTSDIIVIIEVYGVPNDHMKQQSRAVKNNAFSPRWNETFTFLIQVPELALIRFVVETQGFLSGNELLGQYTLPVLCMNKGYRRVPLFSKSGANLEPSSLFIYVWYYRE

Target ORF information:

RefSeq Version	XM_017592771.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C, zeta 1 (Plcz1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592771.1

ORF Insert Sequence:

ATGAATTGTG AACACAGGAT CATGGCCTGC CTGGTCCAGT TTATGGAAGG AACTGAAGTG 
GAATGGACCA CACAAAGGAA ATACGAAAAT GACAGACATA ACCAAGGAAG AATCACCACC 
GAAGATTTTA GAACCATCTA TCGGTGTATT GTACATAGAG AAGAGATCGT TGAGATTTTC 
AACACGTATA CTGAAAACAG GAAAATTCTC CCCGAGGACA GTCTGATTGA ATTTCTAACC 
CAAGAGCAGT ATGAAATGGA GATGGATGAG TCCAGTTCAG TGGAGATCAT CCAGAAGTAC 
GAGCCCATTG CAGAAGTAAA GAACGAGCGG CAGATGTCAA TTGAAGGTTT TGCAAGATAC 
ATGTTTTCTT CAGAATGTCT ACTGTTTAAA GAGACGTGTA ACACAGTGTA CCAAGATATG 
AATAAGCCAC TAAATGATTA CTATATTTCA TCGTCTCACA ACACATATTT GATATCTGAT 
CAAATATTGG GACCAAGTGA CATTTGGGGA TATATAAGTG CCCTCGTGAA AGGCTGCCGC 
TGTCTGGAAA TTGACTGCTG GGATGGAGCA CAAAATGAAC CCATTGTGTA CCATGGCTAC 
ACTCTCACCA GCAAGCTTCT CTTCAAAACC GTTATCCAAG CAATAAACAA GTACGCCTTC 
GTGACGTCTG ATTACCCAGT GGTGCTGTCC TTAGAGAATC ACTGCTCCCC TGGTCAACAG 
GAAGTGATGA CCGACATTCT GCAGAGTACC TTTGGAGACT TTCTGCTCTC AGACATACTT 
GACGAGTTTC CAGACAGTTT GCCATCTCCA GAGGCACTGA AATTTAAAAT ATTAGTGAAA 
AATAAGAAAG TTGGAACCTT ATCTGAAACC CGCGAGAGGC TGGGGACTGA CAAAAGGGGC 
ATAGCGCTAG ACTTGGAAGA AGAAATCTAT GAAAATGAAG ACGAAGACTC AGGAAAGGAG 
CCAGAAACGT GGGATGATTT CCTGTCACGG GTTAAGGAGG AGCAGGAGGC AGACCCCTCA 
ACGTTGAGCG GAATAGCAGA TGCCAAGAAA AAGATCAGGA AGCTAAGAGT AGCTCTGGCC 
TTATCTGATC TTGTCATTTA TACCAAAGCT GAGAAGTTCC GAAACTTCCA ATATTCAAGA 
GTCTATCAGC AGTTTAATGA GACCACTTCT ATGGGAGAGT CTCGAGCTCG AAAACTTTCA 
AAATTGAGAG CCCATGAGTT TATTTTCCAC ACTGCAGCGT TCATCACCAG AGTGTACCCC 
AAGTTCACGA GAGCAGACTC TTCTAATTTT AATCCTCAAG AGTTTTGGAA TGTGGGCTGT 
CAGATGGTGG CCTTGAATTT TCAAACCCCT GGACTGCCTA TGGATTTGCA AAACGGGAAA 
TTTTTGGATA ATGGAGGCTC TGGATATGTT TTGAAGCCAG ACTTTCTTAG AGACACAACT 
TTGGGCTTTA ACCCAAATGA ACCAGAAGGA GATGGCCATC CGGTTACCCT CACGATCCGA 
CTCATCAGTG GGATCCAGTT GCCTGTTAAC GTGCCCTCAA ATACATCTGA CATAATAGTG 
ATCATAGAAG TCTACGGTGT CCCAAACGAC CACATGAAGC AGCAGAGTCG TGCCGTTAAG 
AACAATGCTT TTAGTCCAAG GTGGAATGAA ACATTCACAT TTCTTATTCA AGTGCCAGAA 
TTGGCACTGA TACGTTTCGT TGTTGAAACT CAAGGCTTCC TATCGGGAAA TGAATTACTT 
GGGCAGTACA CTTTACCCGT TCTTTGCATG AACAAAGGTT ATCGTCGCGT TCCTCTGTTT 
TCCAAATCCG GTGCGAACCT TGAACCTTCC TCTCTGTTTA TTTACGTTTG GTACTACAGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Male infertility-linked point mutation reveals a vital binding role for the C2 domain of sperm PLC?.
The Biochemical journal474(6)1003-1016(2017 03)
Nomikos M,Stamatiadis P,Sanders JR,Beck K,Calver BL,Buntwal L,Lofty M,Sideratou Z,Swann K,Lai FA

Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.
Human molecular genetics25(5)878-91(2016 Mar)
Escoffier J,Lee HC,Yassine S,Zouari R,Martinez G,Karaouz?ne T,Coutton C,Kherraf ZE,Halouani L,Triki C,Nef S,Thierry-Mieg N,Savinov SN,Fissore R,Ray PF,Arnoult C

Functional disparity between human PAWP and PLC? in the generation of Ca2+ oscillations for oocyte activation.
Molecular human reproduction21(9)702-10(2015 Sep)
Nomikos M,Sanders JR,Kashir J,Sanusi R,Buntwal L,Love D,Ashley P,Sanders D,Knaggs P,Bunkheila A,Swann K,Lai FA

Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish.
Biology of reproduction78(6)1081-90(2008 Jun)
Ito M,Shikano T,Oda S,Horiguchi T,Tanimoto S,Awaji T,Mitani H,Miyazaki S

Recombinant phospholipase Czeta has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggs.
The Journal of biological chemistry279(11)10408-12(2004 Mar)
Kouchi Z,Fukami K,Shikano T,Oda S,Nakamura Y,Takenawa T,Miyazaki S

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Plcz1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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