Ly96 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ly96
Entrez Gene ID	448830
Full Name	lymphocyte antigen 96
Synonyms	MD-2,MD2
General protein information	Preferred Names lymphocyte antigen 96 Names lymphocyte antigen 96 LPS co-receptor MD-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017593673.1	XP_017449162.1	lymphocyte antigen 96 isoform X1
XM_017602947.1	XP_017458436.1	lymphocyte antigen 96 isoform X2
NM_001024279.1	NP_001019450.1	lymphocyte antigen 96 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	LY96	NP_056179.3
Mus musculus (house mouse)	Ly96	NP_058619.1
Rattus norvegicus (Norway rat)	Ly96	XP_006237788.1
	LY96	NP_001123904.1
Canis lupus familiaris (dog)	LY96	XP_853138.1
Pan troglodytes (chimpanzee)	LY96	NP_001123946.1
Bos taurus (cattle)	LY96	NP_001039982.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of myeloid differentiation factor-2 attenuates obesity-induced cardiomyopathy and fibrosis.
Fang Q, Wang J, Zhang Y, Wang L, Li W, Han J, Huang W, Liang G, Wang Y
Biochimica et biophysica acta1864(1)252-262(2018 Jan)

Plasminogen activator inhibitor-1 regulates LPS-induced TLR4/MD-2 pathway activation and inflammation in alveolar macrophages.
Ren W, Wang Z, Hua F, Zhu L
Inflammation38(1)384-93(2015 Feb)

The attenuated inflammation of MPL is due to the lack of CD14-dependent tight dimerization of the TLR4/MD2 complex at the plasma membrane.
Tanimura N, Saitoh S, Ohto U, Akashi-Takamura S, Fujimoto Y, Fukase K, Shimizu T, Miyake K
International immunology26(6)307-14(2014 Jun)

Rifampin inhibits Toll-like receptor 4 signaling by targeting myeloid differentiation protein 2 and attenuates neuropathic pain.
Wang X, Grace PM, Pham MN, Cheng K, Strand KA, Smith C, Li J, Watkins LR, Yin H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(7)2713-22(2013 Jul)

Innate immune regulation of Serratia marcescens-induced corneal inflammation and infection.
Zhou R, Zhang R, Sun Y, Platt S, Szczotka-Flynn L, Pearlman E
Investigative ophthalmology & visual science53(11)7382-8(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Up-regulation of MD-2 in alveolar macrophages was attenuated after PAI-1 gene knockdown.
Title: Plasminogen activator inhibitor-1 regulates LPS-induced TLR4/MD-2 pathway activation and inflammation in alveolar macrophages.

Data show that rifampin binds to myeloid differentiation protein 2 (MD-2), the key coreceptor for innate immune TLR4.
Title: Rifampin inhibits Toll-like receptor 4 signaling by targeting myeloid differentiation protein 2 and attenuates neuropathic pain.

LPS induced increases in TNF-alpha, IL-1 beta and IL-6 protein levels in NR8383 cell supernatants was significantly inhibited by MD-2 silencing.
Title: Myeloid differentiation protein 2 silencing decreases LPS-induced cytokine production and TLR4/MyD88 pathway activity in alveolar macrophages.

Higher dose LPS can up-regulate the gene expression of TLR4 and MD-2 in rat alveolar macrophage NR8383 cell line, and promote the secretion of TNF-alpha, IL-6 and IL-1beta.
Title: [The effect of lipopolysaccharide on gene expression of TLR4 and MD-2 in rat alveolar macrophage and its secretion of inflammation cytokines].

high affinity interactions between the palmitoyl-oleoyl-phosphatidylglycerol and the Toll-like receptor 4-interacting proteins CD14 and MD-2.
Title: Anionic pulmonary surfactant phospholipids inhibit inflammatory responses from alveolar macrophages and U937 cells by binding the lipopolysaccharide-interacting proteins CD14 and MD-2.

The following Ly96 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ly96 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06148	NM_001024279.1 Latest version!	Rattus norvegicus lymphocyte antigen 96 (Ly96), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa55418	XM_017602947.1 Latest version!	Rattus norvegicus lymphocyte antigen 96 (Ly96), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ORa55418	XM_017593673.1 Latest version!	Rattus norvegicus lymphocyte antigen 96 (Ly96), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ly96 ( NM_001024279.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001019450.1 Rattus norvegicus lymphocyte antigen 96 (Ly96), mRNA.

Price & Availability↑

CloneID	ORa06148
Clone ID Related Accession (Same CDS sequence)	NM_001024279.1
Accession Version	NM_001024279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-04
Organism	Rattus norvegicus(Norway rat)
Product	lymphocyte antigen 96 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY963291.1. On or before Aug 24, 2014 this sequence version replaced XM_008763483.1, XM_006225164.2, XM_006237726.1, XM_003753993.1, XM_003749879.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY963291.1, FQ234779.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTTGCCAT TTTTTCTCTT TTCGACGCTG CTTTCTCCCA TATTTACTGA ATCTGAGAGG 
CAACAGTGGA TCTGCAACTC CTCCGATGCA ATTATTTCCT ACAGTTACTG TGATCACATG 
AAAATCCCTA TTTCAATTAG TTCTGAACCC TGTATAAGAT TGAAGGGAAC CAATGGATTT 
GTGCATGTTG AGTTCATTCC AAGAGGAAAC TTAAAAAATT TATATTTCAA CCTGTTCATC 
AATATCAACT CCATAGAATT GCCGAAGCGC AAGGAAATTG TTTGCCATGG ATATGATGAT 
GATTATTCTT TTTGCAGAGC TCTGAAAGGG GAGGCTGTCA ACACAGCAAT ACCATTTTCC 
TTCGACGGAA TACTATTTCC TAAGGGCCAT CACAGATGTG TTGCAGAAGC CATTGCTGGG 
GATACAGAAG AAAAGCTGTT TTGTTTGAAT TTCACCATCA TTCACCACCA TAATGTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019450.1
CDS	10..492
Translation	MLPFFLFSTLLSPIFTESERQQWICNSSDAIISYSYCDHMKIPISISSEPCIRLKGTNGFVHVEFIPRGNLKNLYFNLFININSIELPKRKEIVCHGYDDDYSFCRALKGEAVNTAIPFSFDGILFPKGHHRCVAEAIAGDTEEKLFCLNFTIIHHHNVN

Target ORF information:

RefSeq Version	NM_001024279.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lymphocyte antigen 96 (Ly96), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024279.1

ORF Insert Sequence:

ATGTTGCCAT TTTTTCTCTT TTCGACGCTG CTTTCTCCCA TATTTACTGA ATCTGAGAGG 
CAACAGTGGA TCTGCAACTC CTCCGATGCA ATTATTTCCT ACAGTTACTG TGATCACATG 
AAAATCCCTA TTTCAATTAG TTCTGAACCC TGTATAAGAT TGAAGGGAAC CAATGGATTT 
GTGCATGTTG AGTTCATTCC AAGAGGAAAC TTAAAAAATT TATATTTCAA CCTGTTCATC 
AATATCAACT CCATAGAATT GCCGAAGCGC AAGGAAATTG TTTGCCATGG ATATGATGAT 
GATTATTCTT TTTGCAGAGC TCTGAAAGGG GAGGCTGTCA ACACAGCAAT ACCATTTTCC 
TTCGACGGAA TACTATTTCC TAAGGGCCAT CACAGATGTG TTGCAGAAGC CATTGCTGGG 
GATACAGAAG AAAAGCTGTT TTGTTTGAAT TTCACCATCA TTCACCACCA TAATGTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ly96 ( XM_017593673.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449162.1 Rattus norvegicus lymphocyte antigen 96 (Ly96), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa55418
Clone ID Related Accession (Same CDS sequence)	XM_017593673.1 , XM_017602947.1
Accession Version	XM_017593673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lymphocyte antigen 96 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) and transcript sequence (FQ234779.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 242 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTGCCAT TTTTTCTCTT TTCGACGCTG CTTTCTCCCA TATTTACTGA ATCTGAGAGG 
CAACAGTGGA TCTGCAACTC CTCCGATGCA ATTATTTCCT ACAGTTACTG TGATCACATG 
AAAATCCCTA TTTCAATTAG TTCTGAACCC TGTATAAGAT TGAAGGGAAC CAATGGATTT 
GTGCATGTTG AGTTCATTCC AAGAGGAAAC TTAAAAAATT TATATTTCAA CCTGTTCATC 
AATATCAACT CCATAGAATT GCCGAAGCGC AAGGAAATTG TTTGCCATGG ATATGATGAT 
GATTATTCTT TTTGCAGAGC TCTGAAAGGG GAGGCTGTCA ACACAGCAAT ACCATTTTCC 
TTCGACGGAA TACTATTTCC TAAGGGCCAT CACAGATGTG TTGCAGAAGC CATTGCTGGG 
GATACAGAGG AAAAGCTGTT TTGTTTGAAT TTCACCATCA TTCACCACCA TAATGTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449162.1
CDS	41..523
Translation	MLPFFLFSTLLSPIFTESERQQWICNSSDAIISYSYCDHMKIPISISSEPCIRLKGTNGFVHVEFIPRGNLKNLYFNLFININSIELPKRKEIVCHGYDDDYSFCRALKGEAVNTAIPFSFDGILFPKGHHRCVAEAIAGDTEEKLFCLNFTIIHHHNVN

Target ORF information:

RefSeq Version	XM_017593673.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lymphocyte antigen 96 (Ly96), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593673.1

ORF Insert Sequence:

ATGTTGCCAT TTTTTCTCTT TTCGACGCTG CTTTCTCCCA TATTTACTGA ATCTGAGAGG 
CAACAGTGGA TCTGCAACTC CTCCGATGCA ATTATTTCCT ACAGTTACTG TGATCACATG 
AAAATCCCTA TTTCAATTAG TTCTGAACCC TGTATAAGAT TGAAGGGAAC CAATGGATTT 
GTGCATGTTG AGTTCATTCC AAGAGGAAAC TTAAAAAATT TATATTTCAA CCTGTTCATC 
AATATCAACT CCATAGAATT GCCGAAGCGC AAGGAAATTG TTTGCCATGG ATATGATGAT 
GATTATTCTT TTTGCAGAGC TCTGAAAGGG GAGGCTGTCA ACACAGCAAT ACCATTTTCC 
TTCGACGGAA TACTATTTCC TAAGGGCCAT CACAGATGTG TTGCAGAAGC CATTGCTGGG 
GATACAGAGG AAAAGCTGTT TTGTTTGAAT TTCACCATCA TTCACCACCA TAATGTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ly96 ( XM_017602947.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458436.1 Rattus norvegicus lymphocyte antigen 96 (Ly96), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa55418
Clone ID Related Accession (Same CDS sequence)	XM_017593673.1 , XM_017602947.1
Accession Version	XM_017602947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lymphocyte antigen 96 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000073.1) and transcript sequence (AY963291.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 121 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTGCCAT TTTTTCTCTT TTCGACGCTG CTTTCTCCCA TATTTACTGA ATCTGAGAGG 
CAACAGTGGA TCTGCAACTC CTCCGATGCA ATTATTTCCT ACAGTTACTG TGATCACATG 
AAAATCCCTA TTTCAATTAG TTCTGAACCC TGTATAAGAT TGAAGGGAAC CAATGGATTT 
GTGCATGTTG AGTTCATTCC AAGAGGAAAC TTAAAAAATT TATATTTCAA CCTGTTCATC 
AATATCAACT CCATAGAATT GCCGAAGCGC AAGGAAATTG TTTGCCATGG ATATGATGAT 
GATTATTCTT TTTGCAGAGC TCTGAAAGGG GAGGCTGTCA ACACAGCAAT ACCATTTTCC 
TTCGACGGAA TACTATTTCC TAAGGGCCAT CACAGATGTG TTGCAGAAGC CATTGCTGGG 
GATACAGAGG AAAAGCTGTT TTGTTTGAAT TTCACCATCA TTCACCACCA TAATGTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458436.1
CDS	10..492
Translation	MLPFFLFSTLLSPIFTESERQQWICNSSDAIISYSYCDHMKIPISISSEPCIRLKGTNGFVHVEFIPRGNLKNLYFNLFININSIELPKRKEIVCHGYDDDYSFCRALKGEAVNTAIPFSFDGILFPKGHHRCVAEAIAGDTEEKLFCLNFTIIHHHNVN

Target ORF information:

RefSeq Version	XM_017602947.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lymphocyte antigen 96 (Ly96), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017602947.1

ORF Insert Sequence:

ATGTTGCCAT TTTTTCTCTT TTCGACGCTG CTTTCTCCCA TATTTACTGA ATCTGAGAGG 
CAACAGTGGA TCTGCAACTC CTCCGATGCA ATTATTTCCT ACAGTTACTG TGATCACATG 
AAAATCCCTA TTTCAATTAG TTCTGAACCC TGTATAAGAT TGAAGGGAAC CAATGGATTT 
GTGCATGTTG AGTTCATTCC AAGAGGAAAC TTAAAAAATT TATATTTCAA CCTGTTCATC 
AATATCAACT CCATAGAATT GCCGAAGCGC AAGGAAATTG TTTGCCATGG ATATGATGAT 
GATTATTCTT TTTGCAGAGC TCTGAAAGGG GAGGCTGTCA ACACAGCAAT ACCATTTTCC 
TTCGACGGAA TACTATTTCC TAAGGGCCAT CACAGATGTG TTGCAGAAGC CATTGCTGGG 
GATACAGAGG AAAAGCTGTT TTGTTTGAAT TTCACCATCA TTCACCACCA TAATGTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of myeloid differentiation factor-2 attenuates obesity-induced cardiomyopathy and fibrosis.
Biochimica et biophysica acta1864(1)252-262(2018 Jan)
Fang Q,Wang J,Zhang Y,Wang L,Li W,Han J,Huang W,Liang G,Wang Y

Plasminogen activator inhibitor-1 regulates LPS-induced TLR4/MD-2 pathway activation and inflammation in alveolar macrophages.
Inflammation38(1)384-93(2015 Feb)
Ren W,Wang Z,Hua F,Zhu L

The attenuated inflammation of MPL is due to the lack of CD14-dependent tight dimerization of the TLR4/MD2 complex at the plasma membrane.
International immunology26(6)307-14(2014 Jun)
Tanimura N,Saitoh S,Ohto U,Akashi-Takamura S,Fujimoto Y,Fukase K,Shimizu T,Miyake K

Rifampin inhibits Toll-like receptor 4 signaling by targeting myeloid differentiation protein 2 and attenuates neuropathic pain.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(7)2713-22(2013 Jul)
Wang X,Grace PM,Pham MN,Cheng K,Strand KA,Smith C,Li J,Watkins LR,Yin H

Innate immune regulation of Serratia marcescens-induced corneal inflammation and infection.
Investigative ophthalmology & visual science53(11)7382-8(2012 Oct)
Zhou R,Zhang R,Sun Y,Platt S,Szczotka-Flynn L,Pearlman E

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Ly96 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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