Eml5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eml5
Entrez Gene ID	444982
Full Name	echinoderm microtubule associated protein like 5
General protein information	Preferred Names echinoderm microtubule associated protein like 5 Names echinoderm microtubule-associated protein-like 5 EMAP-5 EMAP-like protein 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764827.2	XP_008763049.1	echinoderm microtubule-associated protein-like 5 isoform X2
XM_017594265.1	XP_017449754.1	echinoderm microtubule-associated protein-like 5 isoform X4
XM_017594264.1	XP_017449753.1	echinoderm microtubule-associated protein-like 5 isoform X1
NM_001003402.2	NP_001003402.2	echinoderm microtubule-associated protein-like 5
XM_003750213.4	XP_003750261.1	echinoderm microtubule-associated protein-like 5 isoform X3

Pathways from BioSystems

Homologies

	EML5	XP_002805220.1
Canis lupus familiaris (dog)	EML5	XP_868412.1
Bos taurus (cattle)	EML5	NP_001193505.1
Pan troglodytes (chimpanzee)	EML5	XP_510110.4
Mus musculus (house mouse)	Eml5	NP_001074660.1
Xenopus tropicalis (tropical clawed frog)	eml5	XP_004917120.1
Homo sapiens (human)	EML5	NP_899243.1
Gallus gallus (chicken)	EML5	XP_421310.2
Rattus norvegicus (Norway rat)	Eml5	NP_001003402.2
Danio rerio (zebrafish)	si:dkey-1f12.3	XP_003200416.1

Gene Ontology
Bibliography

Related articles in PubMed

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Eml5, a novel WD40 domain protein expressed in rat brain.
O'Connor V, Houtman SH, De Zeeuw CI, Bliss TV, French PJ
Gene336(1)127-37(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eml5 may play a role in the regulation of cytoskeletal rearrangements during neuronal development and in adult brain.
Title: Eml5, a novel WD40 domain protein expressed in rat brain.

The following Eml5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eml5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa61692	XM_017594264.1 Latest version!	Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa61693	XM_017594265.1 Latest version!	Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa09849	NM_001003402.2 Latest version!	Rattus norvegicus EMAP like 5 (Eml5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa29540	XM_003750213.4 Latest version!	Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa48961	XM_008764827.2 Latest version!	Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eml5 ( XM_017594264.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449753.1 Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61692
Clone ID Related Accession (Same CDS sequence)	XM_017594264.1
Accession Version	XM_017594264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	echinoderm microtubule-associated protein-like 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGACAGGG GGTAGTAAGA 
AGAGGATCCA GGCCTCCAGT GAGTAGGGCC CCACCACAGC CCGAGAAACT TCAGTCAAAC 
AATGTAGGCA AGAAGAAGAG ACCCATAGAG GACCTTGTGT TGGAGCTCGC TTTTGGCTAT 
CGGGGCAGAG ACTGCAGGAA CAATGTGCAC TACTTAAATG ACGGTGATGA TATAATTTAT 
CACACCGCCT CTGTTGGAAT TCTGCACAAT GTTGCCACAG GGACTCAGAG TTTTTATCAA 
GAGCATAATG ATGATATTCT GTGCCTCACT GTAAATCAGC ACCCCAAGTT TATCAACATA 
GTGGCAACTG GCCAAGTAGG TGATTCTGCA GACATGTCAG CTACAGCCCC ATCTGTCCAC 
ATCTGGGATG CAGTGAACAA GCAGACATTG TCTATACTAA GATGCTCCCA TTCAAAGGGC 
GTGTGTTCTG TCAGCTTCAG TGCTACTGGG AAGCTGCTGC TGTCTGTAGG GCTAGACCCA 
GAGCACACTG TTACCATTTG GAGATGGCAG GAAGGGGCCA AAATTGCCAG CAGAGGTGGG 
CACAACCAAC GTATTTTTGT AGCAGAATTT CGACCAGATT CAGATACCCA GTTTGTCTCT 
GTGGGTATAA AGCACGTGAA GTTCTGGACC CTGGCAGGAA GGGCTCTTCT CAGCAAAAAG 
GGGCTTCTGA GCACCCTGGA GGATGCCCGG ATGCAGACGA TGCTCGCTGT TGCATTTGGT 
GCAAATAACT TGACGTTTAC AGGTACCATC AGTGGTGATG TCTGTGTGTG GAAAGATCAC 
ATATTGTGTA GAGTGGTGGC TAGAGCACAC AATGGGCCTG TGTTTGCCAT GTACACCACC 
CTGCGAGATG GACTGATTGT GACCGGTGGC AAAGAAAGGC CGTCAAAGGA AGGAGGTGCG 
GTTAAACTGT GGGATCAGGA ACTGCGGCGA TGCCGGGCCT TCAGGCTTGA GACGGGACAA 
GTCACAGATT GTGTTCGGTC TGTGTGCAGA GGCAAAGGCA AGATACTAGT TGGAACAAGG 
AATTCGGAAA TAATTGAAGT TGGGGAGAAA AATGCAGCAT GTAACATTTT AGTTAATGGT 
CATGTGGATG GACCAATCTG GGGACTAGCA ACACATCCTT CCAGGGATTT CTTCCTTTCT 
GCTGCTGAAG ATGGAACAGT GAGACTCTGG GACATTGCTG ATAAAAAGAT GCTAAACAAA 
GTGAATTTGG GACATGCTGC TCGGACAGTA TGTTACAGCC CCGAAGGCGA CATGGTGGCT 
ATTGGAATGA AAAATGGAGA ATTTATTATA TTACTTGTGA GTTCTCTAAA AATATGGGGA 
AAGAAGAGAG ACAGGCGATG TGCAATCCAT GATATCAGAT TTAGTCCAGA TTCCCGGTAT 
TTGGCAGTGG GTTCCAGTGA GAACTCGGTG GACTTTTATG ACCTGACATT GGGTCCCACC 
CTTAACAGAA TCAGCTACTG CAAAGACATT CCAAGCTTTG TCATTCAGAT GGACTTCTCT 
GCAGATAGCA GACATCTCCA GGTTTCTAGT GGCTGCTATA AACGGCATGT CTATGAAGTG 
CCTTCAGGAA AACACCTTGT GGATCATGCT GCCATTGACA GGATCACGTG GGCTACCTGG 
ACTAGTATTC TAGGTGATGA AGTTATGGGA ATCTGGTCCA GACATGCTGA GAAGGCAGAT 
GTCACCTGTG CCTGTGTGTC TCACTCAGGA ATCAGCCTTG TGACAGGAGA TGACTTTGGC 
ATGGTTAAAT TATATGACTT TCCATGCCCA GAAAAATTTG CAAAACACAA GAGGTTCTTG 
GGTCATTCCC CTCATGTGAC AAATATTCGA TTTACCAGTG GTGATCGACA TGTTGTCAGT 
GCTGGAGGCG ATGACTGCAG CTTGCTTGTC TGGAAATGTG TACACACGCC TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449753.1
CDS	428..6364
Translation	MAARSAPSCHLRLEWVYGYRGHQCRNNLYYTAAKEIVYFVAGVGVVYSPREHRQKFYRGHSDDIISLALHPERVLVATGQVGKEPYICVWDSYTVQTVSVLKDVHTHGIACLAFDLDGQRLVSVGLDSKNAVCVWDWKRGKMLSMAPGHTDRIFDISWDLYQPNKLVSCGVKHIKFWSLCGNALTPKRGVFGKTGDLQTILCLACARDELTYSGALNGDIYVWKGINLIRTIQGAHTAGIFSMNACEEGFATGGRDGCIRLWDLTFKPITVIDLRETEQGYKGLSVRSVCWRGDHILVGTQDSEIFEIVVHERNKPFLIMQGHCEGELWALAVHPTKPLAVTGSDDRSVRIWSLVDHALIARCNMDEPIRCAAVNADGVHLALGMKDGSLTVLRVRDMTEVVHIKDRKEAIHELKYSPDGTYLAVGCNDSSVDIYGVAQRYKKVGECVGSLSFITHLDWSSDSKYLQTNDGSGKRLLYKMPGGKEVTSKEEVKGMHWASWTCVAGLEVNGIWPKYSDINDINSVDGNYVGQVLVTADDYGVVKLFRYPCLRKGAKFRKYIGHSAHVTNVRWSHDYQWVISIGGADHSVFQWKFIPERKLKDALHIAPQESLTESNSDESDSDLSDVPELDSEIEQETQLTYHRQVYKEDLPQLKEQCKEKQKSATSKRRERAPGNSIRLHFIHGYRGYDCRSNLFYTQIGEIVYHVAAVGVIYNRQQNTQRFYLGHDDDILCLAIHPLKDYVATGQVGRDPSIHVWDTETIKPLSILKGYHQYGICAVDFSADGKRLASVGIDDSHTIVLWDWKKGEKLSVARGSKDKIFVVKMNPYVPDKLITAGIKHMKFWRRAGGGLIGRKGYVGTLGKNDTMMCAVYGWTEEMAFSGTSTGDVCIWRDVFLVKTVKAHDGPVFSMHALEKGFVTGGKDGVVALWDDSFERCLKTYAIKRADLAPGSKGLLLEDNPSIRAISLGHGHILVGTKNGEILEVDKSGPVTLLVQGHMEGEVWGLATHPYLPICATVSDDKTLRIWDLSPSHCMLAVRKLKKGGRCCCFSPDGKALAVGLNDGSFLMANADTLEDLVSFHHRKDVISDIQFSPGSGKYLAVASHDSFVDIYNVTSSKRVGVCKGATSYITHIDWDSRGKLLQVNTGAKEQLFFEAPRGKKQTIPSVEVEKISWATWTSVLGLCCEGIWPIIGEVTDVTASCLTSDKMVLATGDDLGFVKLFRYPAKGKFGKFKKYVAHSTHVTNVRWTYDDSMLVTLGGADMSLMVWTSEVERHREKKNCDSEESDTDSEEDGGYDSDVTRENEISYTIRALSTNIRPMFGVKPHLQQKEPSVDERQGVVRRGSRPPVSRAPPQPEKLQSNNVGKKKRPIEDLVLELAFGYRGRDCRNNVHYLNDGDDIIYHTASVGILHNVATGTQSFYQEHNDDILCLTVNQHPKFINIVATGQVGDSADMSATAPSVHIWDAVNKQTLSILRCSHSKGVCSVSFSATGKLLLSVGLDPEHTVTIWRWQEGAKIASRGGHNQRIFVAEFRPDSDTQFVSVGIKHVKFWTLAGRALLSKKGLLSTLEDARMQTMLAVAFGANNLTFTGTISGDVCVWKDHILCRVVARAHNGPVFAMYTTLRDGLIVTGGKERPSKEGGAVKLWDQELRRCRAFRLETGQVTDCVRSVCRGKGKILVGTRNSEIIEVGEKNAACNILVNGHVDGPIWGLATHPSRDFFLSAAEDGTVRLWDIADKKMLNKVNLGHAARTVCYSPEGDMVAIGMKNGEFIILLVSSLKIWGKKRDRRCAIHDIRFSPDSRYLAVGSSENSVDFYDLTLGPTLNRISYCKDIPSFVIQMDFSADSRHLQVSSGCYKRHVYEVPSGKHLVDHAAIDRITWATWTSILGDEVMGIWSRHAEKADVTCACVSHSGISLVTGDDFGMVKLYDFPCPEKFAKHKRFLGHSPHVTNIRFTSGDRHVVSAGGDDCSLLVWKCVHTPH

Target ORF information:

RefSeq Version	XM_017594264.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594264.1

ORF Insert Sequence:

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGACAGGG GGTAGTAAGA 
AGAGGATCCA GGCCTCCAGT GAGTAGGGCC CCACCACAGC CCGAGAAACT TCAGTCAAAC 
AATGTAGGCA AGAAGAAGAG ACCCATAGAG GACCTTGTGT TGGAGCTCGC TTTTGGCTAT 
CGGGGCAGAG ACTGCAGGAA CAATGTGCAC TACTTAAATG ACGGTGATGA TATAATTTAT 
CACACCGCCT CTGTTGGAAT TCTGCACAAT GTTGCCACAG GGACTCAGAG TTTTTATCAA 
GAGCATAATG ATGATATTCT GTGCCTCACT GTAAATCAGC ACCCCAAGTT TATCAACATA 
GTGGCAACTG GCCAAGTAGG TGATTCTGCA GACATGTCAG CTACAGCCCC ATCTGTCCAC 
ATCTGGGATG CAGTGAACAA GCAGACATTG TCTATACTAA GATGCTCCCA TTCAAAGGGC 
GTGTGTTCTG TCAGCTTCAG TGCTACTGGG AAGCTGCTGC TGTCTGTAGG GCTAGACCCA 
GAGCACACTG TTACCATTTG GAGATGGCAG GAAGGGGCCA AAATTGCCAG CAGAGGTGGG 
CACAACCAAC GTATTTTTGT AGCAGAATTT CGACCAGATT CAGATACCCA GTTTGTCTCT 
GTGGGTATAA AGCACGTGAA GTTCTGGACC CTGGCAGGAA GGGCTCTTCT CAGCAAAAAG 
GGGCTTCTGA GCACCCTGGA GGATGCCCGG ATGCAGACGA TGCTCGCTGT TGCATTTGGT 
GCAAATAACT TGACGTTTAC AGGTACCATC AGTGGTGATG TCTGTGTGTG GAAAGATCAC 
ATATTGTGTA GAGTGGTGGC TAGAGCACAC AATGGGCCTG TGTTTGCCAT GTACACCACC 
CTGCGAGATG GACTGATTGT GACCGGTGGC AAAGAAAGGC CGTCAAAGGA AGGAGGTGCG 
GTTAAACTGT GGGATCAGGA ACTGCGGCGA TGCCGGGCCT TCAGGCTTGA GACGGGACAA 
GTCACAGATT GTGTTCGGTC TGTGTGCAGA GGCAAAGGCA AGATACTAGT TGGAACAAGG 
AATTCGGAAA TAATTGAAGT TGGGGAGAAA AATGCAGCAT GTAACATTTT AGTTAATGGT 
CATGTGGATG GACCAATCTG GGGACTAGCA ACACATCCTT CCAGGGATTT CTTCCTTTCT 
GCTGCTGAAG ATGGAACAGT GAGACTCTGG GACATTGCTG ATAAAAAGAT GCTAAACAAA 
GTGAATTTGG GACATGCTGC TCGGACAGTA TGTTACAGCC CCGAAGGCGA CATGGTGGCT 
ATTGGAATGA AAAATGGAGA ATTTATTATA TTACTTGTGA GTTCTCTAAA AATATGGGGA 
AAGAAGAGAG ACAGGCGATG TGCAATCCAT GATATCAGAT TTAGTCCAGA TTCCCGGTAT 
TTGGCAGTGG GTTCCAGTGA GAACTCGGTG GACTTTTATG ACCTGACATT GGGTCCCACC 
CTTAACAGAA TCAGCTACTG CAAAGACATT CCAAGCTTTG TCATTCAGAT GGACTTCTCT 
GCAGATAGCA GACATCTCCA GGTTTCTAGT GGCTGCTATA AACGGCATGT CTATGAAGTG 
CCTTCAGGAA AACACCTTGT GGATCATGCT GCCATTGACA GGATCACGTG GGCTACCTGG 
ACTAGTATTC TAGGTGATGA AGTTATGGGA ATCTGGTCCA GACATGCTGA GAAGGCAGAT 
GTCACCTGTG CCTGTGTGTC TCACTCAGGA ATCAGCCTTG TGACAGGAGA TGACTTTGGC 
ATGGTTAAAT TATATGACTT TCCATGCCCA GAAAAATTTG CAAAACACAA GAGGTTCTTG 
GGTCATTCCC CTCATGTGAC AAATATTCGA TTTACCAGTG GTGATCGACA TGTTGTCAGT 
GCTGGAGGCG ATGACTGCAG CTTGCTTGTC TGGAAATGTG TACACACGCC TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml5 ( XM_017594265.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449754.1 Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X4, mRNA.

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CloneID	ORa61693
Clone ID Related Accession (Same CDS sequence)	XM_017594265.1
Accession Version	XM_017594265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	echinoderm microtubule-associated protein-like 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGACAGGG GGTAGTAAGA 
AGAGGATCCA GGCCTCCAGT GAGTAGGGCC CCACCACAGC CCGAGAAACT TCAGTCAAAC 
AATGTAGGCA AGAAGAAGAG ACCCATAGAG GACCTTGTGT TGGAGCTCGC TTTTGGCTAT 
CGGGGCAGAG ACTGCAGGAA CAATGTGCAC TACTTAAATG ACGGTGATGA TATAATTTAT 
CACACCGCCT CTGTTGGAAT TCTGCACAAT GTTGCCACAG GGACTCAGAG TTTTTATCAA 
GAGCATAATG ATGATATTCT GTGCCTCACT GTAAATCAGC ACCCCAAGTT TATCAACATA 
GTGGCAACTG GCCAAGTAGG TGATTCTGCA GACATGTCAG CTACAGCCCC ATCTGTCCAC 
ATCTGGGATG CAGTGAACAA GCAGACATTG TCTATACTAA GATGCTCCCA TTCAAAGGGC 
GTGTGTTCTG TCAGCTTCAG TGCTACTGGG AAGCTGCTGC TGTCTGTAGG GCTAGACCCA 
GAGCACACTG TTACCATTTG GAGATGGCAG GAAGGGGCCA AAATTGCCAG CAGAGGTGGG 
CACAACCAAC GTATTTTTGT AGCAGAATTT CGACCAGATT CAGATACCCA GTTTGTCTCT 
GTGGGTATAA AGCACGTGAA GTTCTGGACC CTGGCAGGAA GGGCTCTTCT CAGCAAAAAG 
GGGCTTCTGA GCACCCTGGA GGATGCCCGG ATGCAGACGA TGCTCGCTGT TGCATTTGGT 
GCAGTACCAT CAGTGGTGAT GTCTGTGTGT GGAAAGATCA CATATTGTGT AGAGTGGTGG 
CTAGAGCACA CAATGGGCCT GTGTTTGCCA TGTACACCAC CCTGCGAGAT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449754.1
CDS	429..5285
Translation	MAARSAPSCHLRLEWVYGYRGHQCRNNLYYTAAKEIVYFVAGVGVVYSPREHRQKFYRGHSDDIISLALHPERVLVATGQVGKEPYICVWDSYTVQTVSVLKDVHTHGIACLAFDLDGQRLVSVGLDSKNAVCVWDWKRGKMLSMAPGHTDRIFDISWDLYQPNKLVSCGVKHIKFWSLCGNALTPKRGVFGKTGDLQTILCLACARDELTYSGALNGDIYVWKGINLIRTIQGAHTAGIFSMNACEEGFATGGRDGCIRLWDLTFKPITVIDLRETEQGYKGLSVRSVCWRGDHILVGTQDSEIFEIVVHERNKPFLIMQGHCEGELWALAVHPTKPLAVTGSDDRSVRIWSLVDHALIARCNMDEPIRCAAVNADGVHLALGMKDGSLTVLRVRDMTEVVHIKDRKEAIHELKYSPDGTYLAVGCNDSSVDIYGVAQRYKKVGECVGSLSFITHLDWSSDSKYLQTNDGSGKRLLYKMPGGKEVTSKEEVKGMHWASWTCVAGLEVNGIWPKYSDINDINSVDGNYVGQVLVTADDYGVVKLFRYPCLRKGAKFRKYIGHSAHVTNVRWSHDYQWVISIGGADHSVFQWKFIPERKLKDALHIAPQESLTESNSDESDSDLSDVPELDSEIEQETQLTYHRQVYKEDLPQLKEQCKEKQKSATSKRRERAPGNSIRLHFIHGYRGYDCRSNLFYTQIGEIVYHVAAVGVIYNRQQNTQRFYLGHDDDILCLAIHPLKDYVATGQVGRDPSIHVWDTETIKPLSILKGYHQYGICAVDFSADGKRLASVGIDDSHTIVLWDWKKGEKLSVARGSKDKIFVVKMNPYVPDKLITAGIKHMKFWRRAGGGLIGRKGYVGTLGKNDTMMCAVYGWTEEMAFSGTSTGDVCIWRDVFLVKTVKAHDGPVFSMHALEKGFVTGGKDGVVALWDDSFERCLKTYAIKRADLAPGSKGLLLEDNPSIRAISLGHGHILVGTKNGEILEVDKSGPVTLLVQGHMEGEVWGLATHPYLPICATVSDDKTLRIWDLSPSHCMLAVRKLKKGGRCCCFSPDGKALAVGLNDGSFLMANADTLEDLVSFHHRKDVISDIQFSPGSGKYLAVASHDSFVDIYNVTSSKRVGVCKGATSYITHIDWDSRGKLLQVNTGAKEQLFFEAPRGKKQTIPSVEVEKISWATWTSVLGLCCEGIWPIIGEVTDVTASCLTSDKMVLATGDDLGFVKLFRYPAKGKFGKFKKYVAHSTHVTNVRWTYDDSMLVTLGGADMSLMVWTSEVERHREKKNCDSEESDTDSEEDGGYDSDVTRENEISYTIRALSTNIRPMFGVKPHLQQKEPSVDERQGVVRRGSRPPVSRAPPQPEKLQSNNVGKKKRPIEDLVLELAFGYRGRDCRNNVHYLNDGDDIIYHTASVGILHNVATGTQSFYQEHNDDILCLTVNQHPKFINIVATGQVGDSADMSATAPSVHIWDAVNKQTLSILRCSHSKGVCSVSFSATGKLLLSVGLDPEHTVTIWRWQEGAKIASRGGHNQRIFVAEFRPDSDTQFVSVGIKHVKFWTLAGRALLSKKGLLSTLEDARMQTMLAVAFGAVPSVVMSVCGKITYCVEWWLEHTMGLCLPCTPPCEMD

Target ORF information:

RefSeq Version	XM_017594265.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594265.1

ORF Insert Sequence:

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGACAGGG GGTAGTAAGA 
AGAGGATCCA GGCCTCCAGT GAGTAGGGCC CCACCACAGC CCGAGAAACT TCAGTCAAAC 
AATGTAGGCA AGAAGAAGAG ACCCATAGAG GACCTTGTGT TGGAGCTCGC TTTTGGCTAT 
CGGGGCAGAG ACTGCAGGAA CAATGTGCAC TACTTAAATG ACGGTGATGA TATAATTTAT 
CACACCGCCT CTGTTGGAAT TCTGCACAAT GTTGCCACAG GGACTCAGAG TTTTTATCAA 
GAGCATAATG ATGATATTCT GTGCCTCACT GTAAATCAGC ACCCCAAGTT TATCAACATA 
GTGGCAACTG GCCAAGTAGG TGATTCTGCA GACATGTCAG CTACAGCCCC ATCTGTCCAC 
ATCTGGGATG CAGTGAACAA GCAGACATTG TCTATACTAA GATGCTCCCA TTCAAAGGGC 
GTGTGTTCTG TCAGCTTCAG TGCTACTGGG AAGCTGCTGC TGTCTGTAGG GCTAGACCCA 
GAGCACACTG TTACCATTTG GAGATGGCAG GAAGGGGCCA AAATTGCCAG CAGAGGTGGG 
CACAACCAAC GTATTTTTGT AGCAGAATTT CGACCAGATT CAGATACCCA GTTTGTCTCT 
GTGGGTATAA AGCACGTGAA GTTCTGGACC CTGGCAGGAA GGGCTCTTCT CAGCAAAAAG 
GGGCTTCTGA GCACCCTGGA GGATGCCCGG ATGCAGACGA TGCTCGCTGT TGCATTTGGT 
GCAGTACCAT CAGTGGTGAT GTCTGTGTGT GGAAAGATCA CATATTGTGT AGAGTGGTGG 
CTAGAGCACA CAATGGGCCT GTGTTTGCCA TGTACACCAC CCTGCGAGAT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml5 ( NM_001003402.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001003402.2 Rattus norvegicus EMAP like 5 (Eml5), mRNA.

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CloneID	ORa09849
Clone ID Related Accession (Same CDS sequence)	NM_001003402.2
Accession Version	NM_001003402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-12
Organism	Rattus norvegicus(Norway rat)
Product	echinoderm microtubule-associated protein-like 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC117104.4 and AC098929.7. On Aug 30, 2012 this sequence version replaced NM_001003402.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGACAGGG GGTAGTAAGA 
GGATCCAGGC CTCCAGTGAG TAGGGCCCCA CCACAGCCCG AGAAACTTCA GTCAAACAAT 
GTAGGCAAGA AGAAGAGACC CATAGAGGAC CTTGTGTTGG AGCTCGCTTT TGGCTATCGG 
GGCAGAGACT GCAGGAACAA TGTGCACTAC TTAAATGACG GTGATGATAT AATTTATCAC 
ACCGCCTCTG TTGGAATTCT GCACAATGTT GCCACAGGGA CTCAGAGTTT TTATCAAGAG 
CATAATGATG ATATTCTGTG CCTCACTGTA AATCAGCACC CCAAGTTTAT CAACATAGTG 
GCAACTGGCC AAGTAGGTGA TTCTGCAGAC ATGTCAGCTA CAGCCCCATC TGTCCACATC 
TGGGATGCAG TGAACAAGCA GACATTGTCT ATACTAAGAT GCTCCCATTC AAAGGGCGTG 
TGTTCTGTCA GCTTCAGTGC TACTGGGAAG CTGCTGCTGT CTGTAGGGCT AGACCCAGAG 
CACACTGTTA CCATTTGGAG ATGGCAGGAA GGGGCCAAAA TTGCCAGCAG AGGTGGGCAC 
AACCAACGTA TTTTTGTAGC AGAATTTCGA CCAGATTCAG ATACCCAGTT TGTCTCTGTG 
GGTATAAAGC ACGTGAAGTT CTGGACCCTG GCAGGAAGGG CTCTTCTCAG CAAAAAGGGG 
CTTCTGAGCA CCCTGGAGGA TGCCCGGATG CAGACGATGC TCGCTGTTGC ATTTGGTGCA 
AATAACTTGA CGTTTACAGG TACCATCAGT GGTGATGTCT GTGTGTGGAA AGATCACATA 
TTGTGTAGAG TGGTGGCTAG AGCACACAAT GGGCCTGTGT TTGCCATGTA CACCACCCTG 
CGAGATGGAC TGATTGTGAC CGGTGGCAAA GAAAGGCCGT CAAAGGAAGG AGGTGCGGTT 
AAACTGTGGG ATCAGGAACT GCGGCGATGC CGGGCCTTCA GGCTTGAGAC GGGACAAGTC 
ACAGATTGTG TTCGGTCTGT GTGCAGAGGC AAAGGCAAGA TACTAGTTGG AACAAGGAAT 
TCGGAAATAA TTGAAGTTGG GGAGAAAAAT GCAGCATGTA ACATTTTAGT TAATGGTCAT 
GTGGATGGAC CAATCTGGGG ACTAGCAACA CATCCTTCCA GGGATTTCTT CCTTTCTGCT 
GCTGAAGATG GAACAGTGAG ACTCTGGGAC ATTGCTGATA AAAAGATGCT AAACAAAGTG 
AATTTGGGAC ATGCTGCTCG GACAGTATGT TACAGCCCCG AAGGCGACAT GGTGGCTATT 
GGAATGAAAA ATGGAGAATT TATTATATTA CTTGTGAGTT CTCTAAAAAT ATGGGGAAAG 
AAGAGAGACA GGCGATGTGC AATCCATGAT ATCAGATTTA GTCCAGATTC CCGGTATTTG 
GCAGTGGGTT CCAGTGAGAA CTCGGTGGAC TTTTATGACC TGACATTGGG TCCCACCCTT 
AACAGAATCA GCTACTGCAA AGACATTCCA AGCTTTGTCA TTCAGATGGA CTTCTCTGCA 
GATAGCAGAC ATCTCCAGGT TTCTAGTGGC TGCTATAAAC GGCATGTCTA TGAAGTGCCT 
TCAGGAAAAC ACCTTGTGGA TCATGCTGCC ATTGACAGGA TCACGTGGGC TACCTGGACT 
AGTATTCTAG GTGATGAAGT TATGGGAATC TGGTCCAGAC ATGCTGAGAA GGCAGATGTC 
ACCTGTGCCT GTGTGTCTCA CTCAGGAATC AGCCTTGTGA CAGGAGATGA CTTTGGCATG 
GTTAAATTAT ATGACTTTCC ATGCCCAGAA AAATTTGCAA AACACAAGAG GTTCTTGGGT 
CATTCCCCTC ATGTGACAAA TATTCGATTT ACCAGTGGTG ATCGACATGT TGTCAGTGCT 
GGAGGCGATG ACTGCAGCTT GCTTGTCTGG AAATGTGTAC ACACGCCTCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003402.2
CDS	380..6313
Translation	MAARSAPSCHLRLEWVYGYRGHQCRNNLYYTAAKEIVYFVAGVGVVYSPREHRQKFYRGHSDDIISLALHPERVLVATGQVGKEPYICVWDSYTVQTVSVLKDVHTHGIACLAFDLDGQRLVSVGLDSKNAVCVWDWKRGKMLSMAPGHTDRIFDISWDLYQPNKLVSCGVKHIKFWSLCGNALTPKRGVFGKTGDLQTILCLACARDELTYSGALNGDIYVWKGINLIRTIQGAHTAGIFSMNACEEGFATGGRDGCIRLWDLTFKPITVIDLRETEQGYKGLSVRSVCWRGDHILVGTQDSEIFEIVVHERNKPFLIMQGHCEGELWALAVHPTKPLAVTGSDDRSVRIWSLVDHALIARCNMDEPIRCAAVNADGVHLALGMKDGSLTVLRVRDMTEVVHIKDRKEAIHELKYSPDGTYLAVGCNDSSVDIYGVAQRYKKVGECVGSLSFITHLDWSSDSKYLQTNDGSGKRLLYKMPGGKEVTSKEEVKGMHWASWTCVAGLEVNGIWPKYSDINDINSVDGNYVGQVLVTADDYGVVKLFRYPCLRKGAKFRKYIGHSAHVTNVRWSHDYQWVISIGGADHSVFQWKFIPERKLKDALHIAPQESLTESNSDESDSDLSDVPELDSEIEQETQLTYHRQVYKEDLPQLKEQCKEKQKSATSKRRERAPGNSIRLHFIHGYRGYDCRSNLFYTQIGEIVYHVAAVGVIYNRQQNTQRFYLGHDDDILCLAIHPLKDYVATGQVGRDPSIHVWDTETIKPLSILKGYHQYGICAVDFSADGKRLASVGIDDSHTIVLWDWKKGEKLSVARGSKDKIFVVKMNPYVPDKLITAGIKHMKFWRRAGGGLIGRKGYVGTLGKNDTMMCAVYGWTEEMAFSGTSTGDVCIWRDVFLVKTVKAHDGPVFSMHALEKGFVTGGKDGVVALWDDSFERCLKTYAIKRADLAPGSKGLLLEDNPSIRAISLGHGHILVGTKNGEILEVDKSGPVTLLVQGHMEGEVWGLATHPYLPICATVSDDKTLRIWDLSPSHCMLAVRKLKKGGRCCCFSPDGKALAVGLNDGSFLMANADTLEDLVSFHHRKDVISDIQFSPGSGKYLAVASHDSFVDIYNVTSSKRVGVCKGATSYITHIDWDSRGKLLQVNTGAKEQLFFEAPRGKKQTIPSVEVEKISWATWTSVLGLCCEGIWPIIGEVTDVTASCLTSDKMVLATGDDLGFVKLFRYPAKGKFGKFKKYVAHSTHVTNVRWTYDDSMLVTLGGADMSLMVWTSEVERHREKKNCDSEESDTDSEEDGGYDSDVTRENEISYTIRALSTNIRPMFGVKPHLQQKEPSVDERQGVVRGSRPPVSRAPPQPEKLQSNNVGKKKRPIEDLVLELAFGYRGRDCRNNVHYLNDGDDIIYHTASVGILHNVATGTQSFYQEHNDDILCLTVNQHPKFINIVATGQVGDSADMSATAPSVHIWDAVNKQTLSILRCSHSKGVCSVSFSATGKLLLSVGLDPEHTVTIWRWQEGAKIASRGGHNQRIFVAEFRPDSDTQFVSVGIKHVKFWTLAGRALLSKKGLLSTLEDARMQTMLAVAFGANNLTFTGTISGDVCVWKDHILCRVVARAHNGPVFAMYTTLRDGLIVTGGKERPSKEGGAVKLWDQELRRCRAFRLETGQVTDCVRSVCRGKGKILVGTRNSEIIEVGEKNAACNILVNGHVDGPIWGLATHPSRDFFLSAAEDGTVRLWDIADKKMLNKVNLGHAARTVCYSPEGDMVAIGMKNGEFIILLVSSLKIWGKKRDRRCAIHDIRFSPDSRYLAVGSSENSVDFYDLTLGPTLNRISYCKDIPSFVIQMDFSADSRHLQVSSGCYKRHVYEVPSGKHLVDHAAIDRITWATWTSILGDEVMGIWSRHAEKADVTCACVSHSGISLVTGDDFGMVKLYDFPCPEKFAKHKRFLGHSPHVTNIRFTSGDRHVVSAGGDDCSLLVWKCVHTPH

Target ORF information:

RefSeq Version	NM_001003402.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus EMAP like 5 (Eml5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003402.2

ORF Insert Sequence:

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGACAGGG GGTAGTAAGA 
GGATCCAGGC CTCCAGTGAG TAGGGCCCCA CCACAGCCCG AGAAACTTCA GTCAAACAAT 
GTAGGCAAGA AGAAGAGACC CATAGAGGAC CTTGTGTTGG AGCTCGCTTT TGGCTATCGG 
GGCAGAGACT GCAGGAACAA TGTGCACTAC TTAAATGACG GTGATGATAT AATTTATCAC 
ACCGCCTCTG TTGGAATTCT GCACAATGTT GCCACAGGGA CTCAGAGTTT TTATCAAGAG 
CATAATGATG ATATTCTGTG CCTCACTGTA AATCAGCACC CCAAGTTTAT CAACATAGTG 
GCAACTGGCC AAGTAGGTGA TTCTGCAGAC ATGTCAGCTA CAGCCCCATC TGTCCACATC 
TGGGATGCAG TGAACAAGCA GACATTGTCT ATACTAAGAT GCTCCCATTC AAAGGGCGTG 
TGTTCTGTCA GCTTCAGTGC TACTGGGAAG CTGCTGCTGT CTGTAGGGCT AGACCCAGAG 
CACACTGTTA CCATTTGGAG ATGGCAGGAA GGGGCCAAAA TTGCCAGCAG AGGTGGGCAC 
AACCAACGTA TTTTTGTAGC AGAATTTCGA CCAGATTCAG ATACCCAGTT TGTCTCTGTG 
GGTATAAAGC ACGTGAAGTT CTGGACCCTG GCAGGAAGGG CTCTTCTCAG CAAAAAGGGG 
CTTCTGAGCA CCCTGGAGGA TGCCCGGATG CAGACGATGC TCGCTGTTGC ATTTGGTGCA 
AATAACTTGA CGTTTACAGG TACCATCAGT GGTGATGTCT GTGTGTGGAA AGATCACATA 
TTGTGTAGAG TGGTGGCTAG AGCACACAAT GGGCCTGTGT TTGCCATGTA CACCACCCTG 
CGAGATGGAC TGATTGTGAC CGGTGGCAAA GAAAGGCCGT CAAAGGAAGG AGGTGCGGTT 
AAACTGTGGG ATCAGGAACT GCGGCGATGC CGGGCCTTCA GGCTTGAGAC GGGACAAGTC 
ACAGATTGTG TTCGGTCTGT GTGCAGAGGC AAAGGCAAGA TACTAGTTGG AACAAGGAAT 
TCGGAAATAA TTGAAGTTGG GGAGAAAAAT GCAGCATGTA ACATTTTAGT TAATGGTCAT 
GTGGATGGAC CAATCTGGGG ACTAGCAACA CATCCTTCCA GGGATTTCTT CCTTTCTGCT 
GCTGAAGATG GAACAGTGAG ACTCTGGGAC ATTGCTGATA AAAAGATGCT AAACAAAGTG 
AATTTGGGAC ATGCTGCTCG GACAGTATGT TACAGCCCCG AAGGCGACAT GGTGGCTATT 
GGAATGAAAA ATGGAGAATT TATTATATTA CTTGTGAGTT CTCTAAAAAT ATGGGGAAAG 
AAGAGAGACA GGCGATGTGC AATCCATGAT ATCAGATTTA GTCCAGATTC CCGGTATTTG 
GCAGTGGGTT CCAGTGAGAA CTCGGTGGAC TTTTATGACC TGACATTGGG TCCCACCCTT 
AACAGAATCA GCTACTGCAA AGACATTCCA AGCTTTGTCA TTCAGATGGA CTTCTCTGCA 
GATAGCAGAC ATCTCCAGGT TTCTAGTGGC TGCTATAAAC GGCATGTCTA TGAAGTGCCT 
TCAGGAAAAC ACCTTGTGGA TCATGCTGCC ATTGACAGGA TCACGTGGGC TACCTGGACT 
AGTATTCTAG GTGATGAAGT TATGGGAATC TGGTCCAGAC ATGCTGAGAA GGCAGATGTC 
ACCTGTGCCT GTGTGTCTCA CTCAGGAATC AGCCTTGTGA CAGGAGATGA CTTTGGCATG 
GTTAAATTAT ATGACTTTCC ATGCCCAGAA AAATTTGCAA AACACAAGAG GTTCTTGGGT 
CATTCCCCTC ATGTGACAAA TATTCGATTT ACCAGTGGTG ATCGACATGT TGTCAGTGCT 
GGAGGCGATG ACTGCAGCTT GCTTGTCTGG AAATGTGTAC ACACGCCTCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml5 ( XM_003750213.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003750261.1 Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X3, mRNA.

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CloneID	ORa29540
Clone ID Related Accession (Same CDS sequence)	XM_003750213.4
Accession Version	XM_003750213.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	echinoderm microtubule-associated protein-like 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003750213.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGGCCTCC AGTGAGTAGG 
GCCCCACCAC AGCCCGAGAA ACTTCAGTCA AACAATGTAG GCAAGAAGAA GAGACCCATA 
GAGGACCTTG TGTTGGAGCT CGCTTTTGGC TATCGGGGCA GAGACTGCAG GAACAATGTG 
CACTACTTAA ATGACGGTGA TGATATAATT TATCACACCG CCTCTGTTGG AATTCTGCAC 
AATGTTGCCA CAGGGACTCA GAGTTTTTAT CAAGAGCATA ATGATGATAT TCTGTGCCTC 
ACTGTAAATC AGCACCCCAA GTTTATCAAC ATAGTGGCAA CTGGCCAAGT AGGTGATTCT 
GCAGACATGT CAGCTACAGC CCCATCTGTC CACATCTGGG ATGCAGTGAA CAAGCAGACA 
TTGTCTATAC TAAGATGCTC CCATTCAAAG GGCGTGTGTT CTGTCAGCTT CAGTGCTACT 
GGGAAGCTGC TGCTGTCTGT AGGGCTAGAC CCAGAGCACA CTGTTACCAT TTGGAGATGG 
CAGGAAGGGG CCAAAATTGC CAGCAGAGGT GGGCACAACC AACGTATTTT TGTAGCAGAA 
TTTCGACCAG ATTCAGATAC CCAGTTTGTC TCTGTGGGTA TAAAGCACGT GAAGTTCTGG 
ACCCTGGCAG GAAGGGCTCT TCTCAGCAAA AAGGGGCTTC TGAGCACCCT GGAGGATGCC 
CGGATGCAGA CGATGCTCGC TGTTGCATTT GGTGCAAATA ACTTGACGTT TACAGGTACC 
ATCAGTGGTG ATGTCTGTGT GTGGAAAGAT CACATATTGT GTAGAGTGGT GGCTAGAGCA 
CACAATGGGC CTGTGTTTGC CATGTACACC ACCCTGCGAG ATGGACTGAT TGTGACCGGT 
GGCAAAGAAA GGCCGTCAAA GGAAGGAGGT GCGGTTAAAC TGTGGGATCA GGAACTGCGG 
CGATGCCGGG CCTTCAGGCT TGAGACGGGA CAAGTCACAG ATTGTGTTCG GTCTGTGTGC 
AGAGGCAAAG GCAAGATACT AGTTGGAACA AGGAATTCGG AAATAATTGA AGTTGGGGAG 
AAAAATGCAG CATGTAACAT TTTAGTTAAT GGTCATGTGG ATGGACCAAT CTGGGGACTA 
GCAACACATC CTTCCAGGGA TTTCTTCCTT TCTGCTGCTG AAGATGGAAC AGTGAGACTC 
TGGGACATTG CTGATAAAAA GATGCTAAAC AAAGTGAATT TGGGACATGC TGCTCGGACA 
GTATGTTACA GCCCCGAAGG CGACATGGTG GCTATTGGAA TGAAAAATGG AGAATTTATT 
ATATTACTTG TGAGTTCTCT AAAAATATGG GGAAAGAAGA GAGACAGGCG ATGTGCAATC 
CATGATATCA GATTTAGTCC AGATTCCCGG TATTTGGCAG TGGGTTCCAG TGAGAACTCG 
GTGGACTTTT ATGACCTGAC ATTGGGTCCC ACCCTTAACA GAATCAGCTA CTGCAAAGAC 
ATTCCAAGCT TTGTCATTCA GATGGACTTC TCTGCAGATA GCAGACATCT CCAGGTTTCT 
AGTGGCTGCT ATAAACGGCA TGTCTATGAA GTGCCTTCAG GAAAACACCT TGTGGATCAT 
GCTGCCATTG ACAGGATCAC GTGGGCTACC TGGACTAGTA TTCTAGGTGA TGAAGTTATG 
GGAATCTGGT CCAGACATGC TGAGAAGGCA GATGTCACCT GTGCCTGTGT GTCTCACTCA 
GGAATCAGCC TTGTGACAGG AGATGACTTT GGCATGGTTA AATTATATGA CTTTCCATGC 
CCAGAAAAAT TTGCAAAACA CAAGAGGTTC TTGGGTCATT CCCCTCATGT GACAAATATT 
CGATTTACCA GTGGTGATCG ACATGTTGTC AGTGCTGGAG GCGATGACTG CAGCTTGCTT 
GTCTGGAAAT GTGTACACAC GCCTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003750261.1
CDS	428..6337
Translation	MAARSAPSCHLRLEWVYGYRGHQCRNNLYYTAAKEIVYFVAGVGVVYSPREHRQKFYRGHSDDIISLALHPERVLVATGQVGKEPYICVWDSYTVQTVSVLKDVHTHGIACLAFDLDGQRLVSVGLDSKNAVCVWDWKRGKMLSMAPGHTDRIFDISWDLYQPNKLVSCGVKHIKFWSLCGNALTPKRGVFGKTGDLQTILCLACARDELTYSGALNGDIYVWKGINLIRTIQGAHTAGIFSMNACEEGFATGGRDGCIRLWDLTFKPITVIDLRETEQGYKGLSVRSVCWRGDHILVGTQDSEIFEIVVHERNKPFLIMQGHCEGELWALAVHPTKPLAVTGSDDRSVRIWSLVDHALIARCNMDEPIRCAAVNADGVHLALGMKDGSLTVLRVRDMTEVVHIKDRKEAIHELKYSPDGTYLAVGCNDSSVDIYGVAQRYKKVGECVGSLSFITHLDWSSDSKYLQTNDGSGKRLLYKMPGGKEVTSKEEVKGMHWASWTCVAGLEVNGIWPKYSDINDINSVDGNYVGQVLVTADDYGVVKLFRYPCLRKGAKFRKYIGHSAHVTNVRWSHDYQWVISIGGADHSVFQWKFIPERKLKDALHIAPQESLTESNSDESDSDLSDVPELDSEIEQETQLTYHRQVYKEDLPQLKEQCKEKQKSATSKRRERAPGNSIRLHFIHGYRGYDCRSNLFYTQIGEIVYHVAAVGVIYNRQQNTQRFYLGHDDDILCLAIHPLKDYVATGQVGRDPSIHVWDTETIKPLSILKGYHQYGICAVDFSADGKRLASVGIDDSHTIVLWDWKKGEKLSVARGSKDKIFVVKMNPYVPDKLITAGIKHMKFWRRAGGGLIGRKGYVGTLGKNDTMMCAVYGWTEEMAFSGTSTGDVCIWRDVFLVKTVKAHDGPVFSMHALEKGFVTGGKDGVVALWDDSFERCLKTYAIKRADLAPGSKGLLLEDNPSIRAISLGHGHILVGTKNGEILEVDKSGPVTLLVQGHMEGEVWGLATHPYLPICATVSDDKTLRIWDLSPSHCMLAVRKLKKGGRCCCFSPDGKALAVGLNDGSFLMANADTLEDLVSFHHRKDVISDIQFSPGSGKYLAVASHDSFVDIYNVTSSKRVGVCKGATSYITHIDWDSRGKLLQVNTGAKEQLFFEAPRGKKQTIPSVEVEKISWATWTSVLGLCCEGIWPIIGEVTDVTASCLTSDKMVLATGDDLGFVKLFRYPAKGKFGKFKKYVAHSTHVTNVRWTYDDSMLVTLGGADMSLMVWTSEVERHREKKNCDSEESDTDSEEDGGYDSDVTRENEISYTIRALSTNIRPMFGVKPHLQQKEPSVDERPPVSRAPPQPEKLQSNNVGKKKRPIEDLVLELAFGYRGRDCRNNVHYLNDGDDIIYHTASVGILHNVATGTQSFYQEHNDDILCLTVNQHPKFINIVATGQVGDSADMSATAPSVHIWDAVNKQTLSILRCSHSKGVCSVSFSATGKLLLSVGLDPEHTVTIWRWQEGAKIASRGGHNQRIFVAEFRPDSDTQFVSVGIKHVKFWTLAGRALLSKKGLLSTLEDARMQTMLAVAFGANNLTFTGTISGDVCVWKDHILCRVVARAHNGPVFAMYTTLRDGLIVTGGKERPSKEGGAVKLWDQELRRCRAFRLETGQVTDCVRSVCRGKGKILVGTRNSEIIEVGEKNAACNILVNGHVDGPIWGLATHPSRDFFLSAAEDGTVRLWDIADKKMLNKVNLGHAARTVCYSPEGDMVAIGMKNGEFIILLVSSLKIWGKKRDRRCAIHDIRFSPDSRYLAVGSSENSVDFYDLTLGPTLNRISYCKDIPSFVIQMDFSADSRHLQVSSGCYKRHVYEVPSGKHLVDHAAIDRITWATWTSILGDEVMGIWSRHAEKADVTCACVSHSGISLVTGDDFGMVKLYDFPCPEKFAKHKRFLGHSPHVTNIRFTSGDRHVVSAGGDDCSLLVWKCVHTPH

Target ORF information:

RefSeq Version	XM_003750213.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003750213.4

ORF Insert Sequence:

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGGCCTCC AGTGAGTAGG 
GCCCCACCAC AGCCCGAGAA ACTTCAGTCA AACAATGTAG GCAAGAAGAA GAGACCCATA 
GAGGACCTTG TGTTGGAGCT CGCTTTTGGC TATCGGGGCA GAGACTGCAG GAACAATGTG 
CACTACTTAA ATGACGGTGA TGATATAATT TATCACACCG CCTCTGTTGG AATTCTGCAC 
AATGTTGCCA CAGGGACTCA GAGTTTTTAT CAAGAGCATA ATGATGATAT TCTGTGCCTC 
ACTGTAAATC AGCACCCCAA GTTTATCAAC ATAGTGGCAA CTGGCCAAGT AGGTGATTCT 
GCAGACATGT CAGCTACAGC CCCATCTGTC CACATCTGGG ATGCAGTGAA CAAGCAGACA 
TTGTCTATAC TAAGATGCTC CCATTCAAAG GGCGTGTGTT CTGTCAGCTT CAGTGCTACT 
GGGAAGCTGC TGCTGTCTGT AGGGCTAGAC CCAGAGCACA CTGTTACCAT TTGGAGATGG 
CAGGAAGGGG CCAAAATTGC CAGCAGAGGT GGGCACAACC AACGTATTTT TGTAGCAGAA 
TTTCGACCAG ATTCAGATAC CCAGTTTGTC TCTGTGGGTA TAAAGCACGT GAAGTTCTGG 
ACCCTGGCAG GAAGGGCTCT TCTCAGCAAA AAGGGGCTTC TGAGCACCCT GGAGGATGCC 
CGGATGCAGA CGATGCTCGC TGTTGCATTT GGTGCAAATA ACTTGACGTT TACAGGTACC 
ATCAGTGGTG ATGTCTGTGT GTGGAAAGAT CACATATTGT GTAGAGTGGT GGCTAGAGCA 
CACAATGGGC CTGTGTTTGC CATGTACACC ACCCTGCGAG ATGGACTGAT TGTGACCGGT 
GGCAAAGAAA GGCCGTCAAA GGAAGGAGGT GCGGTTAAAC TGTGGGATCA GGAACTGCGG 
CGATGCCGGG CCTTCAGGCT TGAGACGGGA CAAGTCACAG ATTGTGTTCG GTCTGTGTGC 
AGAGGCAAAG GCAAGATACT AGTTGGAACA AGGAATTCGG AAATAATTGA AGTTGGGGAG 
AAAAATGCAG CATGTAACAT TTTAGTTAAT GGTCATGTGG ATGGACCAAT CTGGGGACTA 
GCAACACATC CTTCCAGGGA TTTCTTCCTT TCTGCTGCTG AAGATGGAAC AGTGAGACTC 
TGGGACATTG CTGATAAAAA GATGCTAAAC AAAGTGAATT TGGGACATGC TGCTCGGACA 
GTATGTTACA GCCCCGAAGG CGACATGGTG GCTATTGGAA TGAAAAATGG AGAATTTATT 
ATATTACTTG TGAGTTCTCT AAAAATATGG GGAAAGAAGA GAGACAGGCG ATGTGCAATC 
CATGATATCA GATTTAGTCC AGATTCCCGG TATTTGGCAG TGGGTTCCAG TGAGAACTCG 
GTGGACTTTT ATGACCTGAC ATTGGGTCCC ACCCTTAACA GAATCAGCTA CTGCAAAGAC 
ATTCCAAGCT TTGTCATTCA GATGGACTTC TCTGCAGATA GCAGACATCT CCAGGTTTCT 
AGTGGCTGCT ATAAACGGCA TGTCTATGAA GTGCCTTCAG GAAAACACCT TGTGGATCAT 
GCTGCCATTG ACAGGATCAC GTGGGCTACC TGGACTAGTA TTCTAGGTGA TGAAGTTATG 
GGAATCTGGT CCAGACATGC TGAGAAGGCA GATGTCACCT GTGCCTGTGT GTCTCACTCA 
GGAATCAGCC TTGTGACAGG AGATGACTTT GGCATGGTTA AATTATATGA CTTTCCATGC 
CCAGAAAAAT TTGCAAAACA CAAGAGGTTC TTGGGTCATT CCCCTCATGT GACAAATATT 
CGATTTACCA GTGGTGATCG ACATGTTGTC AGTGCTGGAG GCGATGACTG CAGCTTGCTT 
GTCTGGAAAT GTGTACACAC GCCTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eml5 ( XM_008764827.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763049.1 Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X2, mRNA.

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CloneID	ORa48961
Clone ID Related Accession (Same CDS sequence)	XM_008764827.2
Accession Version	XM_008764827.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	echinoderm microtubule-associated protein-like 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764827.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGACAGGG GGTAGTAAGG 
CCTCCAGTGA GTAGGGCCCC ACCACAGCCC GAGAAACTTC AGTCAAACAA TGTAGGCAAG 
AAGAAGAGAC CCATAGAGGA CCTTGTGTTG GAGCTCGCTT TTGGCTATCG GGGCAGAGAC 
TGCAGGAACA ATGTGCACTA CTTAAATGAC GGTGATGATA TAATTTATCA CACCGCCTCT 
GTTGGAATTC TGCACAATGT TGCCACAGGG ACTCAGAGTT TTTATCAAGA GCATAATGAT 
GATATTCTGT GCCTCACTGT AAATCAGCAC CCCAAGTTTA TCAACATAGT GGCAACTGGC 
CAAGTAGGTG ATTCTGCAGA CATGTCAGCT ACAGCCCCAT CTGTCCACAT CTGGGATGCA 
GTGAACAAGC AGACATTGTC TATACTAAGA TGCTCCCATT CAAAGGGCGT GTGTTCTGTC 
AGCTTCAGTG CTACTGGGAA GCTGCTGCTG TCTGTAGGGC TAGACCCAGA GCACACTGTT 
ACCATTTGGA GATGGCAGGA AGGGGCCAAA ATTGCCAGCA GAGGTGGGCA CAACCAACGT 
ATTTTTGTAG CAGAATTTCG ACCAGATTCA GATACCCAGT TTGTCTCTGT GGGTATAAAG 
CACGTGAAGT TCTGGACCCT GGCAGGAAGG GCTCTTCTCA GCAAAAAGGG GCTTCTGAGC 
ACCCTGGAGG ATGCCCGGAT GCAGACGATG CTCGCTGTTG CATTTGGTGC AAATAACTTG 
ACGTTTACAG GTACCATCAG TGGTGATGTC TGTGTGTGGA AAGATCACAT ATTGTGTAGA 
GTGGTGGCTA GAGCACACAA TGGGCCTGTG TTTGCCATGT ACACCACCCT GCGAGATGGA 
CTGATTGTGA CCGGTGGCAA AGAAAGGCCG TCAAAGGAAG GAGGTGCGGT TAAACTGTGG 
GATCAGGAAC TGCGGCGATG CCGGGCCTTC AGGCTTGAGA CGGGACAAGT CACAGATTGT 
GTTCGGTCTG TGTGCAGAGG CAAAGGCAAG ATACTAGTTG GAACAAGGAA TTCGGAAATA 
ATTGAAGTTG GGGAGAAAAA TGCAGCATGT AACATTTTAG TTAATGGTCA TGTGGATGGA 
CCAATCTGGG GACTAGCAAC ACATCCTTCC AGGGATTTCT TCCTTTCTGC TGCTGAAGAT 
GGAACAGTGA GACTCTGGGA CATTGCTGAT AAAAAGATGC TAAACAAAGT GAATTTGGGA 
CATGCTGCTC GGACAGTATG TTACAGCCCC GAAGGCGACA TGGTGGCTAT TGGAATGAAA 
AATGGAGAAT TTATTATATT ACTTGTGAGT TCTCTAAAAA TATGGGGAAA GAAGAGAGAC 
AGGCGATGTG CAATCCATGA TATCAGATTT AGTCCAGATT CCCGGTATTT GGCAGTGGGT 
TCCAGTGAGA ACTCGGTGGA CTTTTATGAC CTGACATTGG GTCCCACCCT TAACAGAATC 
AGCTACTGCA AAGACATTCC AAGCTTTGTC ATTCAGATGG ACTTCTCTGC AGATAGCAGA 
CATCTCCAGG TTTCTAGTGG CTGCTATAAA CGGCATGTCT ATGAAGTGCC TTCAGGAAAA 
CACCTTGTGG ATCATGCTGC CATTGACAGG ATCACGTGGG CTACCTGGAC TAGTATTCTA 
GGTGATGAAG TTATGGGAAT CTGGTCCAGA CATGCTGAGA AGGCAGATGT CACCTGTGCC 
TGTGTGTCTC ACTCAGGAAT CAGCCTTGTG ACAGGAGATG ACTTTGGCAT GGTTAAATTA 
TATGACTTTC CATGCCCAGA AAAATTTGCA AAACACAAGA GGTTCTTGGG TCATTCCCCT 
CATGTGACAA ATATTCGATT TACCAGTGGT GATCGACATG TTGTCAGTGC TGGAGGCGAT 
GACTGCAGCT TGCTTGTCTG GAAATGTGTA CACACGCCTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763049.1
CDS	428..6352
Translation	MAARSAPSCHLRLEWVYGYRGHQCRNNLYYTAAKEIVYFVAGVGVVYSPREHRQKFYRGHSDDIISLALHPERVLVATGQVGKEPYICVWDSYTVQTVSVLKDVHTHGIACLAFDLDGQRLVSVGLDSKNAVCVWDWKRGKMLSMAPGHTDRIFDISWDLYQPNKLVSCGVKHIKFWSLCGNALTPKRGVFGKTGDLQTILCLACARDELTYSGALNGDIYVWKGINLIRTIQGAHTAGIFSMNACEEGFATGGRDGCIRLWDLTFKPITVIDLRETEQGYKGLSVRSVCWRGDHILVGTQDSEIFEIVVHERNKPFLIMQGHCEGELWALAVHPTKPLAVTGSDDRSVRIWSLVDHALIARCNMDEPIRCAAVNADGVHLALGMKDGSLTVLRVRDMTEVVHIKDRKEAIHELKYSPDGTYLAVGCNDSSVDIYGVAQRYKKVGECVGSLSFITHLDWSSDSKYLQTNDGSGKRLLYKMPGGKEVTSKEEVKGMHWASWTCVAGLEVNGIWPKYSDINDINSVDGNYVGQVLVTADDYGVVKLFRYPCLRKGAKFRKYIGHSAHVTNVRWSHDYQWVISIGGADHSVFQWKFIPERKLKDALHIAPQESLTESNSDESDSDLSDVPELDSEIEQETQLTYHRQVYKEDLPQLKEQCKEKQKSATSKRRERAPGNSIRLHFIHGYRGYDCRSNLFYTQIGEIVYHVAAVGVIYNRQQNTQRFYLGHDDDILCLAIHPLKDYVATGQVGRDPSIHVWDTETIKPLSILKGYHQYGICAVDFSADGKRLASVGIDDSHTIVLWDWKKGEKLSVARGSKDKIFVVKMNPYVPDKLITAGIKHMKFWRRAGGGLIGRKGYVGTLGKNDTMMCAVYGWTEEMAFSGTSTGDVCIWRDVFLVKTVKAHDGPVFSMHALEKGFVTGGKDGVVALWDDSFERCLKTYAIKRADLAPGSKGLLLEDNPSIRAISLGHGHILVGTKNGEILEVDKSGPVTLLVQGHMEGEVWGLATHPYLPICATVSDDKTLRIWDLSPSHCMLAVRKLKKGGRCCCFSPDGKALAVGLNDGSFLMANADTLEDLVSFHHRKDVISDIQFSPGSGKYLAVASHDSFVDIYNVTSSKRVGVCKGATSYITHIDWDSRGKLLQVNTGAKEQLFFEAPRGKKQTIPSVEVEKISWATWTSVLGLCCEGIWPIIGEVTDVTASCLTSDKMVLATGDDLGFVKLFRYPAKGKFGKFKKYVAHSTHVTNVRWTYDDSMLVTLGGADMSLMVWTSEVERHREKKNCDSEESDTDSEEDGGYDSDVTRENEISYTIRALSTNIRPMFGVKPHLQQKEPSVDERQGVVRPPVSRAPPQPEKLQSNNVGKKKRPIEDLVLELAFGYRGRDCRNNVHYLNDGDDIIYHTASVGILHNVATGTQSFYQEHNDDILCLTVNQHPKFINIVATGQVGDSADMSATAPSVHIWDAVNKQTLSILRCSHSKGVCSVSFSATGKLLLSVGLDPEHTVTIWRWQEGAKIASRGGHNQRIFVAEFRPDSDTQFVSVGIKHVKFWTLAGRALLSKKGLLSTLEDARMQTMLAVAFGANNLTFTGTISGDVCVWKDHILCRVVARAHNGPVFAMYTTLRDGLIVTGGKERPSKEGGAVKLWDQELRRCRAFRLETGQVTDCVRSVCRGKGKILVGTRNSEIIEVGEKNAACNILVNGHVDGPIWGLATHPSRDFFLSAAEDGTVRLWDIADKKMLNKVNLGHAARTVCYSPEGDMVAIGMKNGEFIILLVSSLKIWGKKRDRRCAIHDIRFSPDSRYLAVGSSENSVDFYDLTLGPTLNRISYCKDIPSFVIQMDFSADSRHLQVSSGCYKRHVYEVPSGKHLVDHAAIDRITWATWTSILGDEVMGIWSRHAEKADVTCACVSHSGISLVTGDDFGMVKLYDFPCPEKFAKHKRFLGHSPHVTNIRFTSGDRHVVSAGGDDCSLLVWKCVHTPH

Target ORF information:

RefSeq Version	XM_008764827.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus echinoderm microtubule associated protein like 5 (Eml5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764827.2

ORF Insert Sequence:

ATGGCGGCTC GGAGCGCCCC GAGCTGCCAC CTGCGGCTCG AGTGGGTGTA CGGCTACCGG 
GGCCACCAGT GCCGCAACAA CCTCTACTAC ACCGCGGCCA AGGAGATCGT GTACTTCGTG 
GCGGGGGTCG GCGTCGTGTA TAGCCCGCGG GAGCATCGGC AGAAGTTCTA CCGCGGCCAC 
AGCGACGACA TCATCAGTCT CGCACTGCAT CCTGAGCGCG TGTTGGTAGC AACAGGACAA 
GTTGGGAAAG AGCCTTACAT CTGTGTTTGG GATTCTTACA CGGTCCAGAC TGTGTCGGTT 
CTGAAGGACG TTCATACACA TGGTATAGCT TGTTTAGCAT TTGACTTGGA TGGACAGCGC 
TTAGTTTCAG TTGGACTCGA TTCAAAGAAT GCAGTTTGTG TTTGGGACTG GAAAAGGGGG 
AAAATGCTGT CTATGGCTCC TGGTCACACA GACAGAATAT TTGATATTTC TTGGGATTTG 
TACCAGCCAA ATAAGCTCGT CAGCTGCGGT GTTAAACACA TCAAGTTCTG GAGTTTGTGT 
GGAAATGCTC TGACCCCAAA ACGAGGAGTT TTTGGTAAAA CAGGTGACCT TCAGACAATA 
TTGTGCCTAG CCTGTGCTCG GGACGAATTA ACATATTCTG GTGCACTCAA TGGCGATATA 
TATGTTTGGA AAGGAATCAA TCTTATACGA ACAATACAAG GAGCCCATAC TGCAGGGATT 
TTTAGTATGA ATGCTTGTGA AGAAGGCTTT GCTACTGGAG GCAGAGATGG CTGTATTCGT 
CTTTGGGATT TAACTTTTAA ACCAATTACT GTGATTGATC TCAGGGAAAC AGAACAGGGA 
TACAAAGGTT TATCTGTGAG GAGTGTTTGT TGGCGAGGTG ACCACATTTT AGTGGGAACA 
CAGGACAGTG AAATTTTTGA AATTGTTGTG CATGAAAGAA ATAAACCTTT CCTAATTATG 
CAAGGGCATT GCGAAGGTGA ACTTTGGGCG CTTGCTGTTC ACCCTACTAA GCCTTTGGCT 
GTAACCGGAA GTGATGATCG TTCAGTCAGG ATTTGGAGCC TTGTAGATCA TGCTCTAATA 
GCGAGATGTA ATATGGACGA GCCAATCCGA TGTGCCGCTG TCAATGCCGA CGGAGTCCAC 
CTCGCCCTTG GAATGAAGGA TGGGTCACTC ACTGTCCTTA GAGTGAGAGA TATGACTGAA 
GTTGTACATA TTAAAGACAG GAAAGAAGCA ATCCATGAGC TGAAATATTC TCCAGATGGC 
ACTTACCTTG CGGTTGGCTG CAATGACAGC TCAGTTGACA TCTATGGAGT TGCTCAGCGT 
TACAAAAAAG TGGGTGAATG CGTCGGTTCC CTTAGCTTCA TCACTCATTT GGATTGGTCT 
TCAGACAGTA AATACTTACA GACAAATGAC GGCAGTGGGA AACGACTTCT CTACAAGATG 
CCAGGAGGAA AGGAAGTGAC AAGCAAAGAA GAAGTAAAAG GCATGCACTG GGCTTCATGG 
ACGTGTGTTG CAGGCCTTGA AGTCAATGGA ATTTGGCCCA AGTATTCTGA CATCAATGAT 
ATAAACTCAG TAGATGGAAA CTATGTTGGC CAGGTTTTAG TTACAGCTGA TGACTATGGA 
GTTGTAAAAT TATTCCGATA TCCATGTTTA AGAAAAGGGG CCAAGTTCAG AAAATACATT 
GGCCATTCAG CTCATGTAAC TAATGTCAGA TGGTCACATG ATTACCAGTG GGTTATTTCT 
ATTGGTGGAG CGGATCACTC TGTCTTTCAG TGGAAATTTA TTCCTGAGAG AAAATTAAAA 
GATGCTCTTC ACATAGCACC GCAAGAAAGT CTGACTGAGT CCAACAGCGA TGAATCAGAT 
TCAGATCTGT CTGATGTTCC GGAACTAGAT TCTGAGATTG AGCAAGAGAC ACAGCTTACT 
TACCACCGGC AGGTTTACAA AGAAGATCTA CCTCAACTCA AAGAACAATG CAAAGAGAAG 
CAGAAAAGTG CTACTTCTAA ACGAAGAGAA CGTGCTCCAG GAAATAGTAT TCGATTACAC 
TTCATTCATG GTTACAGAGG TTATGACTGT CGAAGCAATC TTTTCTACAC TCAAATTGGT 
GAAATTGTGT ACCATGTAGC AGCAGTGGGT GTCATATATA ATAGACAACA GAACACTCAG 
CGTTTTTACT TGGGTCATGA TGATGATATC CTGTGTCTGG CTATTCATCC TTTGAAAGAC 
TATGTGGCAA CAGGCCAGGT TGGTAGAGAT CCCTCAATTC ACGTCTGGGA CACAGAAACC 
ATTAAACCAT TGTCTATATT AAAGGGCTAC CACCAATATG GCATCTGTGC TGTGGATTTC 
TCAGCTGATG GGAAACGCCT AGCTTCAGTT GGAATAGATG ACAGCCACAC CATTGTATTG 
TGGGATTGGA AGAAAGGGGA GAAACTTTCA GTGGCAAGAG GCAGTAAAGA TAAGATTTTT 
GTTGTGAAAA TGAACCCCTA TGTGCCTGAT AAACTAATTA CAGCTGGGAT TAAACACATG 
AAATTTTGGC GTAGAGCAGG GGGAGGGCTA ATTGGAAGAA AAGGCTACGT CGGCACTCTG 
GGGAAAAATG ACACAATGAT GTGTGCAGTG TATGGATGGA CTGAAGAGAT GGCTTTTTCT 
GGAACATCCA CAGGAGATGT ATGCATCTGG AGAGATGTCT TTCTTGTAAA AACAGTTAAA 
GCCCATGATG GGCCCGTGTT CAGTATGCAC GCATTGGAGA AAGGATTTGT AACTGGAGGA 
AAAGATGGCG TGGTAGCCCT TTGGGATGAT TCCTTTGAAA GATGTCTCAA GACTTATGCT 
ATAAAAAGAG CTGATTTAGC CCCAGGATCT AAAGGTCTGC TCTTGGAAGA TAACCCATCT 
ATACGTGCCA TATCATTAGG ACATGGTCAT ATTCTGGTTG GCACAAAGAA CGGTGAGATA 
TTGGAAGTGG ATAAAAGTGG CCCAGTCACT CTGCTGGTCC AGGGACACAT GGAAGGAGAG 
GTGTGGGGTT TGGCCACACA TCCTTATCTG CCCATCTGTG CTACTGTAAG TGATGATAAG 
ACCTTAAGAA TATGGGATCT CTCTCCTAGT CATTGTATGT TGGCAGTCCG GAAACTGAAG 
AAGGGTGGGC GGTGCTGCTG CTTTTCCCCT GATGGCAAAG CTTTGGCTGT AGGACTCAAT 
GATGGAAGCT TCTTGATGGC CAACGCAGAC ACTCTAGAGG ATCTTGTGTC CTTTCACCAC 
AGAAAAGATG TTATTTCAGA CATCCAGTTT TCACCTGGTT CTGGGAAATA CCTCGCTGTG 
GCGTCCCATG ACAGCTTCGT TGATATATAC AATGTGACAA GTAGCAAGCG AGTGGGAGTT 
TGCAAAGGAG CAACAAGCTA CATCACCCAC ATCGACTGGG ACAGCAGAGG AAAGCTTTTA 
CAGGTCAACA CTGGTGCTAA AGAACAGCTA TTCTTCGAAG CTCCCAGAGG GAAAAAACAA 
ACCATCCCCA GTGTGGAGGT GGAAAAAATT AGTTGGGCGA CATGGACAAG TGTTCTTGGT 
TTATGTTGTG AGGGAATTTG GCCAATAATT GGAGAAGTCA CAGATGTAAC CGCCTCTTGC 
CTCACCAGTG ACAAAATGGT CCTAGCTACA GGGGATGACT TGGGATTCGT GAAGCTGTTC 
AGATACCCAG CTAAAGGAAA ATTTGGAAAG TTTAAGAAGT ATGTGGCTCA CAGCACACAC 
GTCACAAATG TTCGCTGGAC TTACGATGAC AGCATGCTGG TTACCCTGGG AGGAGCAGAT 
ATGTCCTTAA TGGTATGGAC AAGTGAAGTG GAGAGACATC GGGAAAAGAA GAACTGTGAC 
AGTGAAGAGT CTGATACAGA TTCTGAAGAA GATGGAGGCT ATGACAGCGA TGTTACAAGA 
GAGAATGAAA TTAGCTACAC CATCAGAGCC TTATCAACTA ATATCCGCCC AATGTTTGGA 
GTCAAGCCTC ATTTGCAACA GAAAGAGCCC TCAGTTGATG AAAGACAGGG GGTAGTAAGG 
CCTCCAGTGA GTAGGGCCCC ACCACAGCCC GAGAAACTTC AGTCAAACAA TGTAGGCAAG 
AAGAAGAGAC CCATAGAGGA CCTTGTGTTG GAGCTCGCTT TTGGCTATCG GGGCAGAGAC 
TGCAGGAACA ATGTGCACTA CTTAAATGAC GGTGATGATA TAATTTATCA CACCGCCTCT 
GTTGGAATTC TGCACAATGT TGCCACAGGG ACTCAGAGTT TTTATCAAGA GCATAATGAT 
GATATTCTGT GCCTCACTGT AAATCAGCAC CCCAAGTTTA TCAACATAGT GGCAACTGGC 
CAAGTAGGTG ATTCTGCAGA CATGTCAGCT ACAGCCCCAT CTGTCCACAT CTGGGATGCA 
GTGAACAAGC AGACATTGTC TATACTAAGA TGCTCCCATT CAAAGGGCGT GTGTTCTGTC 
AGCTTCAGTG CTACTGGGAA GCTGCTGCTG TCTGTAGGGC TAGACCCAGA GCACACTGTT 
ACCATTTGGA GATGGCAGGA AGGGGCCAAA ATTGCCAGCA GAGGTGGGCA CAACCAACGT 
ATTTTTGTAG CAGAATTTCG ACCAGATTCA GATACCCAGT TTGTCTCTGT GGGTATAAAG 
CACGTGAAGT TCTGGACCCT GGCAGGAAGG GCTCTTCTCA GCAAAAAGGG GCTTCTGAGC 
ACCCTGGAGG ATGCCCGGAT GCAGACGATG CTCGCTGTTG CATTTGGTGC AAATAACTTG 
ACGTTTACAG GTACCATCAG TGGTGATGTC TGTGTGTGGA AAGATCACAT ATTGTGTAGA 
GTGGTGGCTA GAGCACACAA TGGGCCTGTG TTTGCCATGT ACACCACCCT GCGAGATGGA 
CTGATTGTGA CCGGTGGCAA AGAAAGGCCG TCAAAGGAAG GAGGTGCGGT TAAACTGTGG 
GATCAGGAAC TGCGGCGATG CCGGGCCTTC AGGCTTGAGA CGGGACAAGT CACAGATTGT 
GTTCGGTCTG TGTGCAGAGG CAAAGGCAAG ATACTAGTTG GAACAAGGAA TTCGGAAATA 
ATTGAAGTTG GGGAGAAAAA TGCAGCATGT AACATTTTAG TTAATGGTCA TGTGGATGGA 
CCAATCTGGG GACTAGCAAC ACATCCTTCC AGGGATTTCT TCCTTTCTGC TGCTGAAGAT 
GGAACAGTGA GACTCTGGGA CATTGCTGAT AAAAAGATGC TAAACAAAGT GAATTTGGGA 
CATGCTGCTC GGACAGTATG TTACAGCCCC GAAGGCGACA TGGTGGCTAT TGGAATGAAA 
AATGGAGAAT TTATTATATT ACTTGTGAGT TCTCTAAAAA TATGGGGAAA GAAGAGAGAC 
AGGCGATGTG CAATCCATGA TATCAGATTT AGTCCAGATT CCCGGTATTT GGCAGTGGGT 
TCCAGTGAGA ACTCGGTGGA CTTTTATGAC CTGACATTGG GTCCCACCCT TAACAGAATC 
AGCTACTGCA AAGACATTCC AAGCTTTGTC ATTCAGATGG ACTTCTCTGC AGATAGCAGA 
CATCTCCAGG TTTCTAGTGG CTGCTATAAA CGGCATGTCT ATGAAGTGCC TTCAGGAAAA 
CACCTTGTGG ATCATGCTGC CATTGACAGG ATCACGTGGG CTACCTGGAC TAGTATTCTA 
GGTGATGAAG TTATGGGAAT CTGGTCCAGA CATGCTGAGA AGGCAGATGT CACCTGTGCC 
TGTGTGTCTC ACTCAGGAAT CAGCCTTGTG ACAGGAGATG ACTTTGGCAT GGTTAAATTA 
TATGACTTTC CATGCCCAGA AAAATTTGCA AAACACAAGA GGTTCTTGGG TCATTCCCCT 
CATGTGACAA ATATTCGATT TACCAGTGGT GATCGACATG TTGTCAGTGC TGGAGGCGAT 
GACTGCAGCT TGCTTGTCTG GAAATGTGTA CACACGCCTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Eml5, a novel WD40 domain protein expressed in rat brain.
Gene336(1)127-37(2004 Jul)
O'Connor V,Houtman SH,De Zeeuw CI,Bliss TV,French PJ

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Eml5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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