Adm2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adm2
Entrez Gene ID	399475
Full Name	adrenomedullin 2
Synonyms	Imd
General protein information	Preferred Names adrenomedullin 2 Names ADM2 intermedin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	acts via calcitonin receptor-like receptor (CRLR) and receptor activity-modifying protein (RAMP) protein complexes to induce cAMP production; decreases blood pressure; suppresses gastric emptying activity and food intake [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_201426.1	NP_958829.1	protein ADM2 precursor

Pathways from BioSystems

Homologies

	ADM2	XP_001116328.1
Rattus norvegicus (Norway rat)	Adm2	NP_958829.1
Canis lupus familiaris (dog)	ADM2	XP_848492.2
Pan troglodytes (chimpanzee)	ADM2	XP_001141730.1
Homo sapiens (human)	ADM2	NP_001240774.1
Bos taurus (cattle)	ADM2	NP_001180129.1
Mus musculus (house mouse)	Adm2	NP_891558.1

Gene Ontology
Bibliography

Related articles in PubMed

Intermedin inhibits unilateral ureteral obstruction-induced oxidative stress via NADPH oxidase Nox4 and cAMP-dependent mechanisms.
Qiao X, Wang L, Wang Y, Su X, Qi Y, Fan Y, Peng Z
Renal failure39(1)652-659(2017 Nov)

Effects of intermedin on autophagy in cerebral ischemia/reperfusion injury.
Wang M, Wang J, Liu Z, Guo X, Wang N, Jia N, Zhang Y, Yuan J
Neuropeptides(2017 Nov)

Intermedin reduces neointima formation by regulating vascular smooth muscle cell phenotype via cAMP/PKA pathway.
Zhu Q, Ni XQ, Lu WW, Zhang JS, Ren JL, Wu D, Chen Y, Zhang LS, Yu YR, Tang CS, Qi YF
Atherosclerosis266212-222(2017 Nov)

Calcitonin Peptide Family Members Are Differentially Regulated by LPS and Inhibit Functions of Rat Alveolar NR8383 Macrophages.
Soultanova A, Mikulski Z, Pfeil U, Grau V, Kummer W
PloS one11(10)e0163483(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased expression of IMD and all its receptor components (CRLR, RAMP1, RAMP2, and RAMP3) were displayed within the aorta of rats with Hypercholesterolemia.
Title: Hypercholesterolemia Up-Regulates the Expression of Intermedin and Its Receptor Components in the Aorta of Rats via Inducing the Oxidative Stress.

Rat Alveolar NR8383 Macrophage cell line NR8383 is both a source and a target of the calcitonin peptide family members, ADM, ADM2, and CGRP.
Title: Calcitonin Peptide Family Members Are Differentially Regulated by LPS and Inhibit Functions of Rat Alveolar NR8383 Macrophages.

Intermedin plays a protective role in renal fibrosis via its antioxidant effects.
Title: Intermedin is upregulated and attenuates renal fibrosis by inhibition of oxidative stress in rats with unilateral ureteral obstruction.

These findings are the first direct evidence that IMD protects against cardiac hypertrophy by inhibiting endoplasmic reticulum stress via activating AMP-activated protein kinase.
Title: Intermedin1-53 protects against cardiac hypertrophy by inhibiting endoplasmic reticulum stress via activating AMP-activated protein kinase.

ADM2-like immunoreactivity is localized in granulosa cells, blood vessels, oocytes, cumulous oophorus, and corpus luteum of pregnant ovaries, suggesting a potential role for ADM2 in the ovary
Title: Adrenomedullin2 (ADM2)/intermedin (IMD) in rat ovary: changes in estrous cycle and pregnancy and its role in ovulation and steroidogenesis.

The following Adm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12176	NM_201426.1 Latest version!	Rattus norvegicus adrenomedullin 2 (Adm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adm2 ( NM_201426.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_958829.1 Rattus norvegicus adrenomedullin 2 (Adm2), mRNA.

Price & Availability↑

CloneID	ORa12176
Clone ID Related Accession (Same CDS sequence)	NM_201426.1
Accession Version	NM_201426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	protein ADM2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB121036.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB121036.1, FQ234235.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCAGT TGCTGATGGT CACGGTAACC TTCGGTTGCA TCAGCCTCCT CTACCTGCTC 
CCGGGCACGC TGTCTGGCAG CCTGGGTAAG GGACTGAGAC CCAGAGAACC CCCAGCCAAG 
ATTCCTTCCA GCGGCCCACA GCCTGGACAC CCCTCACTTC GGCCTGTAGT CTGGAAGCCT 
CCACATGCTC TCCAGCCACA GGGAAGGGGC AACCCGGCCC TTGCTACGGT TCATCTGCCT 
CAGGGTGGTG GCTCACGACA CCCAGGTCCC CAGCGTCACG TGGGATCCCG AAGACCCCAT 
GCCCAGCTCC TTCGGGTTGG CTGTGTCCTG GGAACGTGTC AAGTCCAGAA TCTCAGCCAT 
CGCCTGTGGC AACTTGTCCG GCCAAGTGGC CGGCGGGACT CAGCTCCTGT GGATCCCAGC 
AGCCCCCACA GTTATGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958829.1
CDS	173..613
Translation	MAQLLMVTVTFGCISLLYLLPGTLSGSLGKGLRPREPPAKIPSSGPQPGHPSLRPVVWKPPHALQPQGRGNPALATVHLPQGGGSRHPGPQRHVGSRRPHAQLLRVGCVLGTCQVQNLSHRLWQLVRPSGRRDSAPVDPSSPHSYG

Target ORF information:

RefSeq Version	NM_201426.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adrenomedullin 2 (Adm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201426.1

ORF Insert Sequence:

ATGGCCCAGT TGCTGATGGT CACGGTAACC TTCGGTTGCA TCAGCCTCCT CTACCTGCTC 
CCGGGCACGC TGTCTGGCAG CCTGGGTAAG GGACTGAGAC CCAGAGAACC CCCAGCCAAG 
ATTCCTTCCA GCGGCCCACA GCCTGGACAC CCCTCACTTC GGCCTGTAGT CTGGAAGCCT 
CCACATGCTC TCCAGCCACA GGGAAGGGGC AACCCGGCCC TTGCTACGGT TCATCTGCCT 
CAGGGTGGTG GCTCACGACA CCCAGGTCCC CAGCGTCACG TGGGATCCCG AAGACCCCAT 
GCCCAGCTCC TTCGGGTTGG CTGTGTCCTG GGAACGTGTC AAGTCCAGAA TCTCAGCCAT 
CGCCTGTGGC AACTTGTCCG GCCAAGTGGC CGGCGGGACT CAGCTCCTGT GGATCCCAGC 
AGCCCCCACA GTTATGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intermedin inhibits unilateral ureteral obstruction-induced oxidative stress via NADPH oxidase Nox4 and cAMP-dependent mechanisms.
Renal failure39(1)652-659(2017 Nov)
Qiao X,Wang L,Wang Y,Su X,Qi Y,Fan Y,Peng Z

Effects of intermedin on autophagy in cerebral ischemia/reperfusion injury.
Neuropeptides(2017 Nov)
Wang M,Wang J,Liu Z,Guo X,Wang N,Jia N,Zhang Y,Yuan J

Intermedin reduces neointima formation by regulating vascular smooth muscle cell phenotype via cAMP/PKA pathway.
Atherosclerosis266212-222(2017 Nov)
Zhu Q,Ni XQ,Lu WW,Zhang JS,Ren JL,Wu D,Chen Y,Zhang LS,Yu YR,Tang CS,Qi YF

Calcitonin Peptide Family Members Are Differentially Regulated by LPS and Inhibit Functions of Rat Alveolar NR8383 Macrophages.
PloS one11(10)e0163483(2016)
Soultanova A,Mikulski Z,Pfeil U,Grau V,Kummer W