Ptch2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptch2
Entrez Gene ID	366452
Full Name	patched 2
General protein information	Preferred Names patched 2 Names protein patched homolog 2 patched homolog 1 patched homolog 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764050.2	XP_008762272.1	protein patched homolog 2 isoform X1
NM_001108975.2	NP_001102445.2	protein patched homolog 2
XM_008764050.1	XP_008762272.1	protein patched homolog 2 isoform X1
XM_017593539.1	XP_017449028.1	protein patched homolog 2 isoform X2
NM_001108975.1	NP_001102445.1	protein patched homolog 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Lentivirus-mediated silencing of the PTC1 and PTC2 genes promotes recovery from spinal cord injury by activating the Hedgehog signaling pathway in a rat model.
Zhang YD, Zhu ZS, Zhang D, Zhang Z, Ma B, Zhao SC, Xue F
Experimental & molecular medicine49(12)e412(2017 Dec)

Patched 1 and patched 2 redundancy has a key role in regulating epidermal differentiation.
Adolphe C, Nieuwenhuis E, Villani R, Li ZJ, Kaur P, Hui CC, Wainwright BJ
The Journal of investigative dermatology134(7)1981-1990(2014 Jul)

A missense mutation in PTCH2 underlies dominantly inherited NBCCS in a Chinese family.
Fan Z, Li J, Du J, Zhang H, Shen Y, Wang CY, Wang S
Journal of medical genetics45(5)303-8(2008 May)

Mice with a targeted mutation of patched2 are viable but develop alopecia and epidermal hyperplasia.
Nieuwenhuis E, Motoyama J, Barnfield PC, Yoshikawa Y, Zhang X, Mo R, Crackower MA, Hui CC
Molecular and cellular biology26(17)6609-22(2006 Sep)

Characterization of two patched receptors for the vertebrate hedgehog protein family.
Carpenter D, Stone DM, Brush J, Ryan A, Armanini M, Frantz G, Rosenthal A, de Sauvage FJ
Proceedings of the National Academy of Sciences of the United States of America95(23)13630-4(1998 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ptch2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptch2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11896	NM_001108975.1 Latest version!	Rattus norvegicus patched 2 (Ptch2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa61336	XM_017593539.1 Latest version!	Rattus norvegicus patched 2 (Ptch2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
ORa48810	NM_001108975.2 Latest version!	Rattus norvegicus patched 2 (Ptch2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa48810	XM_008764050.2 Latest version!	Rattus norvegicus patched 2 (Ptch2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
ORa48810	XM_008764050.1	Rattus norvegicus patched 2 (Ptch2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptch2 ( NM_001108975.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102445.1 Rattus norvegicus patched 2 (Ptch2), mRNA.

Price & Availability↑

CloneID	ORa11896
Clone ID Related Accession (Same CDS sequence)	NM_001108975.1
Accession Version	NM_001108975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-21
Organism	Rattus norvegicus(Norway rat)
Product	protein patched homolog 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474008.2. On or before Oct 4, 2007 this sequence version replaced XM_345570.3, XM_001071653.1.

ATGCTTGCTG GTTCTAACTT TGACTTCATC ATAAGGTACT TCTTCGTGGT GCTGACGGTG 
CTGACACTCT TGGGCCTGCT CCATGGACTC CTGCTGCTTC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCAC CACAGGTGGT CCAGATATAC AAGGAGAGTC CACAGGCCCT AAGCTCCACA 
GCCCCACAGA GGGGTGGGCT CAGGTGGGGC GTGTCCCCCA CCCTGCCCCA GAGTTTTGCC 
AGAGTGACTA CCTCCATGAC TGTGGCCCTC CACCCACCAC CACTACCTGG AGCATACGTC 
CACCCAGCCT CCGATGAGCC CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102445.1
CDS	3270..3596
Translation	MLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQIYKESPQALSSTAPQRGGLRWGVSPTLPQSFARVTTSMTVALHPPPLPGAYVHPASDEPT

Target ORF information:

RefSeq Version	NM_001108975.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus patched 2 (Ptch2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108975.1

ORF Insert Sequence:

ATGCTTGCTG GTTCTAACTT TGACTTCATC ATAAGGTACT TCTTCGTGGT GCTGACGGTG 
CTGACACTCT TGGGCCTGCT CCATGGACTC CTGCTGCTTC CTGTGCTGCT GTCCATCCTG 
GGCCCCCCAC CACAGGTGGT CCAGATATAC AAGGAGAGTC CACAGGCCCT AAGCTCCACA 
GCCCCACAGA GGGGTGGGCT CAGGTGGGGC GTGTCCCCCA CCCTGCCCCA GAGTTTTGCC 
AGAGTGACTA CCTCCATGAC TGTGGCCCTC CACCCACCAC CACTACCTGG AGCATACGTC 
CACCCAGCCT CCGATGAGCC CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( XM_017593539.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449028.1 Rattus norvegicus patched 2 (Ptch2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa61336
Clone ID Related Accession (Same CDS sequence)	XM_017593539.1
Accession Version	XM_017593539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein patched homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCCCC GTCAGCTGTA TGAGCACTTC CGGGGCGACT ACCAGACACA TGACATCGGC 
TGGAGTGAGG AGCAGGCCAG CATGGTGCTG CAGGCCTGGC AGAGGCGCTT TGTGCAGCTA 
GCCCAGGAGG CTCTGCCAGC CAACGCATCC CAGCAGATCC ATGCTTTCTC CTCCACCACC 
CTGGATGACA TCCTGCAGGC GTTCTCGGAA GTCAGTGCCA CCCGTGTGGT AGGAGGCTAT 
CTGCTCATGC TGGCCTATGC CTGCATAACA ATGCTACGGT GGGACTGCGC CCAGTCCCAG 
GGTGCCGTCG GTCTTGCTGG GGTGTTGCTC GTGGCCCTGG CGGTGGCCTC AGGTCTTGGG 
CTTTGTGCCC TACTTGGCAT CACTTTCAAT GCTGCCACTA CTCAGGTACT GCCCTTCTTA 
GCTCTGGGCA TCGGTGTGGA TGACATCTTC CTGCTGGCAC ATGCATTCAC AAAGGCCCCA 
CCCAACACTC CTCTCCCAGA GCGCATGGGT GAGTGTTTGC GACGCACCGG CACCAGTGTG 
GCACTCACGT CCATCAACAA CATGGTCGCC TTCTTCATGG CTGTTCTGGT TCCCATCCCT 
GCGCTGCGGG CTTTCTCCCT GCAGGCAGCC ATAGTGGTCG GCTGCAACTT TGCAGCGGTA 
ATGCTTGTCT TCCCCGCTGT CCTCAGCCTC GACCTGCGCC GGCGCCACCG CCAGCGCCTC 
GACGTTCTCT GCTGCTTTTC TAGCCCCTGC TCTGCTCAGG TCATTCAGAT GCTGCCCCAG 
GAACTAGGGG ACAGAGCCGT ACCAGTGGGC ATTGCCCACC TGACTGCCAC TGTGCAAGCT 
TTTACCCACT GCGAAGCCAG CAGCCAGCAC GTAGTCACCA TTTTGCCTCC TCAAGCCCAC 
CTGCTGTCTC CAACTTCTGA CCCACTGGGC TCTGAACTCT ACAGCCCTGG GGGGTCCACA 
CGGGACCTTC TCGGTCAGGA GGAGGGGACG AGGCCAAAGG CGTCCTGCAG GTCTCTGCTC 
TGTGCCCACT GGACTCTCGC CCATTTTGCC CGCTATCAGT TTGCGCCTTT ACTGCTCCAG 
ACACAAGCCA AGGCCCTGGT GTTGCTGTTC TTCGGGGCTC TTTTGGGTCT GAGCCTCTAT 
GGAGCCACCT TGGTCCAAGA TGGGCTGGCC CTGACAGATG TGGTCCCTCG GGGCACTAAG 
GAACATGCTT TCCTGAGCGC CCAGCTCAGG TACTTCTCCC TGTACGAGGT GGCCCTGGTG 
ACACAGGGTG GCTTTGACTA CGCCCACTCC CAACGCGCCC TCTTTGATCT GCACCAGCGC 
TTCAGTTCCC TCAAGGCTGT GCTCCCCCCA CCTGCGGCCC AGGCCCCCCG CACCTGGCTG 
CACTACTACC GCAGCTGGCT ACAGGGTATC CAGACCGCAT TTGACCAAGA CTGGGCTTCT 
GGGCGCATCA CCCGCAACTC TTACCGCAAC GGCTCTGAAG ATGGCGCCCT GGCCTACAAG 
CTGCTCATCC AAACCGGGAA TGCCCAGGAA CCTCTGGATT TCAACCAGCT GACCACAAGG 
AAACTGGTGG ACAAGGAGGG ACTCATTCCC CCAGAACTCT TCTACATGGG GCTAACCGTG 
TGGGTGAGCA GCGACCCCCT GGGCCTAGCA GCCTCTCAGG CCAACTTCTA CCCCCCACCT 
CCGGAATGGC TGCATGACAA GTACGATACC ACTGGAGAGA ACCTTCGCAT CCCGGCAGCC 
CAGCCCTTGG AGTTTGCCCA GTTCCCCTTC CTGTTGCATG GACTCCAGAA GACTGCAGAC 
TTTGTGGAAG CCATCGAAGG GGCCCGGGCG GCATGCACAG AGGCAGGCCA GGCAGGGGTG 
CACGCCTACC CCAGTGGCTC TCCCTTTCTC TTCTGGGAGC AGTACCTGGG CCTTCGGCGC 
TGCTTCCTCT TGGCGGTCTG CATCTTGCTG CTCTGCACCT TCCTCGTCTG TGCTCTGCTC 
TTACTCAACC CGTGGACAGC TGGCCTCATA GTGCTGGTCC TGGCGATGAT GACTGTGGAG 
CTCTTTGGTA TCATGGGATT CCTGGGCATC AAACTGAGTG CCATCCCCGT GGTAATCCTC 
GTGGCCTCTG TAGGCATCGG TGTTGAATTC ACGGTTCATG TGGCTCTGGG CTTCCTGACC 
AGCCATGGTA GCCGGAACCT GCGGGCTGCT CGCGCTCTGG AGCAGACCTT TGCCCCAGTG 
ACTGATGGAG CTGTCTCTAC ATTGCTGGGT CTGCTCATGC TTGCTGGTTC TAACTTTGAC 
TTCATCATAA GGTACTTCTT CGTGGTGCTG ACGGTGCTGA CACTCTTGGG CCTGCTCCAT 
GGACTCCTGC TGCTTCCTGT GCTGCTGTCC ATCCTGGGCC CCCCACCACA GGTGGTCCAG 
ATATACAAGG AGAGTCCACA GGCCCTAAGC TCCACAGCCC CACAGAGGGG TGGGCTCAGG 
TGGGGCGTGT CCCCCACCCT GCCCCAGAGT TTTGCCAGAG TGACTACCTC CATGACTGTG 
GCCCTCCACC CACCACCACT ACCTGGAGCA TACGTCCACC CAGCCTCCGA TGAGCCCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449028.1
CDS	115..2697
Translation	MSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILQAFSEVSATRVVGGYLLMLAYACITMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPNTPLPERMGECLRRTGTSVALTSINNMVAFFMAVLVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPTSDPLGSELYSPGGSTRDLLGQEEGTRPKASCRSLLCAHWTLAHFARYQFAPLLLQTQAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPAAQAPRTWLHYYRSWLQGIQTAFDQDWASGRITRNSYRNGSEDGALAYKLLIQTGNAQEPLDFNQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTSHGSRNLRAARALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQIYKESPQALSSTAPQRGGLRWGVSPTLPQSFARVTTSMTVALHPPPLPGAYVHPASDEPT

Target ORF information:

RefSeq Version	XM_017593539.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus patched 2 (Ptch2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593539.1

ORF Insert Sequence:

ATGAGCCCCC GTCAGCTGTA TGAGCACTTC CGGGGCGACT ACCAGACACA TGACATCGGC 
TGGAGTGAGG AGCAGGCCAG CATGGTGCTG CAGGCCTGGC AGAGGCGCTT TGTGCAGCTA 
GCCCAGGAGG CTCTGCCAGC CAACGCATCC CAGCAGATCC ATGCTTTCTC CTCCACCACC 
CTGGATGACA TCCTGCAGGC GTTCTCGGAA GTCAGTGCCA CCCGTGTGGT AGGAGGCTAT 
CTGCTCATGC TGGCCTATGC CTGCATAACA ATGCTACGGT GGGACTGCGC CCAGTCCCAG 
GGTGCCGTCG GTCTTGCTGG GGTGTTGCTC GTGGCCCTGG CGGTGGCCTC AGGTCTTGGG 
CTTTGTGCCC TACTTGGCAT CACTTTCAAT GCTGCCACTA CTCAGGTACT GCCCTTCTTA 
GCTCTGGGCA TCGGTGTGGA TGACATCTTC CTGCTGGCAC ATGCATTCAC AAAGGCCCCA 
CCCAACACTC CTCTCCCAGA GCGCATGGGT GAGTGTTTGC GACGCACCGG CACCAGTGTG 
GCACTCACGT CCATCAACAA CATGGTCGCC TTCTTCATGG CTGTTCTGGT TCCCATCCCT 
GCGCTGCGGG CTTTCTCCCT GCAGGCAGCC ATAGTGGTCG GCTGCAACTT TGCAGCGGTA 
ATGCTTGTCT TCCCCGCTGT CCTCAGCCTC GACCTGCGCC GGCGCCACCG CCAGCGCCTC 
GACGTTCTCT GCTGCTTTTC TAGCCCCTGC TCTGCTCAGG TCATTCAGAT GCTGCCCCAG 
GAACTAGGGG ACAGAGCCGT ACCAGTGGGC ATTGCCCACC TGACTGCCAC TGTGCAAGCT 
TTTACCCACT GCGAAGCCAG CAGCCAGCAC GTAGTCACCA TTTTGCCTCC TCAAGCCCAC 
CTGCTGTCTC CAACTTCTGA CCCACTGGGC TCTGAACTCT ACAGCCCTGG GGGGTCCACA 
CGGGACCTTC TCGGTCAGGA GGAGGGGACG AGGCCAAAGG CGTCCTGCAG GTCTCTGCTC 
TGTGCCCACT GGACTCTCGC CCATTTTGCC CGCTATCAGT TTGCGCCTTT ACTGCTCCAG 
ACACAAGCCA AGGCCCTGGT GTTGCTGTTC TTCGGGGCTC TTTTGGGTCT GAGCCTCTAT 
GGAGCCACCT TGGTCCAAGA TGGGCTGGCC CTGACAGATG TGGTCCCTCG GGGCACTAAG 
GAACATGCTT TCCTGAGCGC CCAGCTCAGG TACTTCTCCC TGTACGAGGT GGCCCTGGTG 
ACACAGGGTG GCTTTGACTA CGCCCACTCC CAACGCGCCC TCTTTGATCT GCACCAGCGC 
TTCAGTTCCC TCAAGGCTGT GCTCCCCCCA CCTGCGGCCC AGGCCCCCCG CACCTGGCTG 
CACTACTACC GCAGCTGGCT ACAGGGTATC CAGACCGCAT TTGACCAAGA CTGGGCTTCT 
GGGCGCATCA CCCGCAACTC TTACCGCAAC GGCTCTGAAG ATGGCGCCCT GGCCTACAAG 
CTGCTCATCC AAACCGGGAA TGCCCAGGAA CCTCTGGATT TCAACCAGCT GACCACAAGG 
AAACTGGTGG ACAAGGAGGG ACTCATTCCC CCAGAACTCT TCTACATGGG GCTAACCGTG 
TGGGTGAGCA GCGACCCCCT GGGCCTAGCA GCCTCTCAGG CCAACTTCTA CCCCCCACCT 
CCGGAATGGC TGCATGACAA GTACGATACC ACTGGAGAGA ACCTTCGCAT CCCGGCAGCC 
CAGCCCTTGG AGTTTGCCCA GTTCCCCTTC CTGTTGCATG GACTCCAGAA GACTGCAGAC 
TTTGTGGAAG CCATCGAAGG GGCCCGGGCG GCATGCACAG AGGCAGGCCA GGCAGGGGTG 
CACGCCTACC CCAGTGGCTC TCCCTTTCTC TTCTGGGAGC AGTACCTGGG CCTTCGGCGC 
TGCTTCCTCT TGGCGGTCTG CATCTTGCTG CTCTGCACCT TCCTCGTCTG TGCTCTGCTC 
TTACTCAACC CGTGGACAGC TGGCCTCATA GTGCTGGTCC TGGCGATGAT GACTGTGGAG 
CTCTTTGGTA TCATGGGATT CCTGGGCATC AAACTGAGTG CCATCCCCGT GGTAATCCTC 
GTGGCCTCTG TAGGCATCGG TGTTGAATTC ACGGTTCATG TGGCTCTGGG CTTCCTGACC 
AGCCATGGTA GCCGGAACCT GCGGGCTGCT CGCGCTCTGG AGCAGACCTT TGCCCCAGTG 
ACTGATGGAG CTGTCTCTAC ATTGCTGGGT CTGCTCATGC TTGCTGGTTC TAACTTTGAC 
TTCATCATAA GGTACTTCTT CGTGGTGCTG ACGGTGCTGA CACTCTTGGG CCTGCTCCAT 
GGACTCCTGC TGCTTCCTGT GCTGCTGTCC ATCCTGGGCC CCCCACCACA GGTGGTCCAG 
ATATACAAGG AGAGTCCACA GGCCCTAAGC TCCACAGCCC CACAGAGGGG TGGGCTCAGG 
TGGGGCGTGT CCCCCACCCT GCCCCAGAGT TTTGCCAGAG TGACTACCTC CATGACTGTG 
GCCCTCCACC CACCACCACT ACCTGGAGCA TACGTCCACC CAGCCTCCGA TGAGCCCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( NM_001108975.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102445.2 Rattus norvegicus patched 2 (Ptch2), mRNA.

Price & Availability↑

CloneID	ORa48810
Clone ID Related Accession (Same CDS sequence)	XM_008764050.2 , NM_001108975.2 , XM_008764050.1
Accession Version	NM_001108975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-05
Organism	Rattus norvegicus(Norway rat)
Product	protein patched homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119459.6. On or before Sep 6, 2019 this sequence version replaced XM_008764050.2, NM_001108975.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. This RefSeq transcript is based on transcript alignments from orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGTTCGGC CACTGTCCCT TGGAGAGCTG CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC AAATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG TGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTAGGACTGG TGGCTTTGGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCATTACACC 
AAAGAGAAGC TGGGGGAGGA GGCTGCCTAC ACCTCCCAGA TGTTGATTCA GACTGCACGC 
CAGGAGGGGG CAAACATCCT CACCCCCGAG GCGCTTGGCT TTCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTACAAGT CTCACTTTAT GGGAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGAATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGTTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA TCCCCAGCAG 
CTCCTAGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG TCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTGGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCGG AGGCCTTCCA GAGCACCTTC 
CTGCTGATGA GCCCCCGTCA GCTGTATGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GTGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCAGCCAAC GCATCCCAGC AGATCCATGC TTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCAGGCGTTC TCGGAAGTCA GTGCCACCCG TGTGGTAGGA 
GGCTATCTGC TCATGCTGGC CTATGCCTGC ATAACAATGC TACGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTCGGTCT TGCTGGGGTG TTGCTCGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACTCA GGTACTGCCC 
TTCTTAGCTC TGGGCATCGG TGTGGATGAC ATCTTCCTGC TGGCACATGC ATTCACAAAG 
GCCCCACCCA ACACTCCTCT CCCAGAGCGC ATGGGTGAGT GTTTGCGACG CACCGGCACC 
AGTGTGGCAC TCACGTCCAT CAACAACATG GTCGCCTTCT TCATGGCTGT TCTGGTTCCC 
ATCCCTGCGC TGCGGGCTTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TTGTCTTCCC CGCTGTCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAG 
CGCCTCGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCTG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAAC TAGGGGACAG AGCCGTACCA GTGGGCATTG CCCACCTGAC TGCCACTGTG 
CAAGCTTTTA CCCACTGCGA AGCCAGCAGC CAGCACGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAAC TTCTGACCCA CTGGGCTCTG AACTCTACAG CCCTGGGGGG 
TCCACACGGG ACCTTCTCGG TCAGGAGGAG GGGACGAGGC CAAAGGCGTC CTGCAGGTCT 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC GCCTTTACTG 
CTCCAGACAC AAGCCAAGGC CCTGGTGTTG CTGTTCTTCG GGGCTCTTTT GGGTCTGAGC 
CTCTATGGAG CCACCTTGGT CCAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTCGGGGC 
ACTAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCTGTGCTC CCCCCACCTG CGGCCCAGGC CCCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGTATCCAGA CCGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCCG CAACTCTTAC CGCAACGGCT CTGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAACCTC TGGATTTCAA CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AACTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTAGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCGG AATGGCTGCA TGACAAGTAC GATACCACTG GAGAGAACCT TCGCATCCCG 
GCAGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTGT TGCATGGACT CCAGAAGACT 
GCAGACTTTG TGGAAGCCAT CGAAGGGGCC CGGGCGGCAT GCACAGAGGC AGGCCAGGCA 
GGGGTGCACG CCTACCCCAG TGGCTCTCCC TTTCTCTTCT GGGAGCAGTA CCTGGGCCTT 
CGGCGCTGCT TCCTCTTGGC GGTCTGCATC TTGCTGCTCT GCACCTTCCT CGTCTGTGCT 
CTGCTCTTAC TCAACCCGTG GACAGCTGGC CTCATAGTGC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGTATCAT GGGATTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTA 
ATCCTCGTGG CCTCTGTAGG CATCGGTGTT GAATTCACGG TTCATGTGGC TCTGGGCTTC 
CTGACCAGCC ATGGTAGCCG GAACCTGCGG GCTGCTCGCG CTCTGGAGCA GACCTTTGCC 
CCAGTGACTG ATGGAGCTGT CTCTACATTG CTGGGTCTGC TCATGCTTGC TGGTTCTAAC 
TTTGACTTCA TCATAAGGTA CTTCTTCGTG GTGCTGACGG TGCTGACACT CTTGGGCCTG 
CTCCATGGAC TCCTGCTGCT TCCTGTGCTG CTGTCCATCC TGGGCCCCCC ACCACAGGTG 
GTCCAGATAT ACAAGGAGAG TCCACAGGCC CTAAGCTCCA CAGCCCCACA GAGGGGTGGG 
CTCAGGTGGG GCGTGTCCCC CACCCTGCCC CAGAGTTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC ACCACTACCT GGAGCATACG TCCACCCAGC CTCCGATGAG 
CCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102445.2
CDS	290..3838
Translation	MVRPLSLGELPPSYTPPARSSAPQILAGSLQAPLWLRAYFQGLLFSLGCRIQKHCGKVLFLGLVALGALALGLRVAVIETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTARQEGANILTPEALGFHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPQQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLDPDDPHCPPSAPNRHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGTARDLQGQLLRAEAFQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILQAFSEVSATRVVGGYLLMLAYACITMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPNTPLPERMGECLRRTGTSVALTSINNMVAFFMAVLVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPTSDPLGSELYSPGGSTRDLLGQEEGTRPKASCRSLLCAHWTLAHFARYQFAPLLLQTQAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPAAQAPRTWLHYYRSWLQGIQTAFDQDWASGRITRNSYRNGSEDGALAYKLLIQTGNAQEPLDFNQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTSHGSRNLRAARALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQIYKESPQALSSTAPQRGGLRWGVSPTLPQSFARVTTSMTVALHPPPLPGAYVHPASDEPT

Target ORF information:

RefSeq Version	NM_001108975.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus patched 2 (Ptch2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108975.2

ORF Insert Sequence:

ATGGTTCGGC CACTGTCCCT TGGAGAGCTG CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC AAATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG TGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTAGGACTGG TGGCTTTGGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCATTACACC 
AAAGAGAAGC TGGGGGAGGA GGCTGCCTAC ACCTCCCAGA TGTTGATTCA GACTGCACGC 
CAGGAGGGGG CAAACATCCT CACCCCCGAG GCGCTTGGCT TTCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTACAAGT CTCACTTTAT GGGAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGAATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGTTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA TCCCCAGCAG 
CTCCTAGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG TCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTGGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCGG AGGCCTTCCA GAGCACCTTC 
CTGCTGATGA GCCCCCGTCA GCTGTATGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GTGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCAGCCAAC GCATCCCAGC AGATCCATGC TTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCAGGCGTTC TCGGAAGTCA GTGCCACCCG TGTGGTAGGA 
GGCTATCTGC TCATGCTGGC CTATGCCTGC ATAACAATGC TACGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTCGGTCT TGCTGGGGTG TTGCTCGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACTCA GGTACTGCCC 
TTCTTAGCTC TGGGCATCGG TGTGGATGAC ATCTTCCTGC TGGCACATGC ATTCACAAAG 
GCCCCACCCA ACACTCCTCT CCCAGAGCGC ATGGGTGAGT GTTTGCGACG CACCGGCACC 
AGTGTGGCAC TCACGTCCAT CAACAACATG GTCGCCTTCT TCATGGCTGT TCTGGTTCCC 
ATCCCTGCGC TGCGGGCTTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TTGTCTTCCC CGCTGTCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAG 
CGCCTCGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCTG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAAC TAGGGGACAG AGCCGTACCA GTGGGCATTG CCCACCTGAC TGCCACTGTG 
CAAGCTTTTA CCCACTGCGA AGCCAGCAGC CAGCACGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAAC TTCTGACCCA CTGGGCTCTG AACTCTACAG CCCTGGGGGG 
TCCACACGGG ACCTTCTCGG TCAGGAGGAG GGGACGAGGC CAAAGGCGTC CTGCAGGTCT 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC GCCTTTACTG 
CTCCAGACAC AAGCCAAGGC CCTGGTGTTG CTGTTCTTCG GGGCTCTTTT GGGTCTGAGC 
CTCTATGGAG CCACCTTGGT CCAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTCGGGGC 
ACTAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCTGTGCTC CCCCCACCTG CGGCCCAGGC CCCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGTATCCAGA CCGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCCG CAACTCTTAC CGCAACGGCT CTGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAACCTC TGGATTTCAA CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AACTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTAGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCGG AATGGCTGCA TGACAAGTAC GATACCACTG GAGAGAACCT TCGCATCCCG 
GCAGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTGT TGCATGGACT CCAGAAGACT 
GCAGACTTTG TGGAAGCCAT CGAAGGGGCC CGGGCGGCAT GCACAGAGGC AGGCCAGGCA 
GGGGTGCACG CCTACCCCAG TGGCTCTCCC TTTCTCTTCT GGGAGCAGTA CCTGGGCCTT 
CGGCGCTGCT TCCTCTTGGC GGTCTGCATC TTGCTGCTCT GCACCTTCCT CGTCTGTGCT 
CTGCTCTTAC TCAACCCGTG GACAGCTGGC CTCATAGTGC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGTATCAT GGGATTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTA 
ATCCTCGTGG CCTCTGTAGG CATCGGTGTT GAATTCACGG TTCATGTGGC TCTGGGCTTC 
CTGACCAGCC ATGGTAGCCG GAACCTGCGG GCTGCTCGCG CTCTGGAGCA GACCTTTGCC 
CCAGTGACTG ATGGAGCTGT CTCTACATTG CTGGGTCTGC TCATGCTTGC TGGTTCTAAC 
TTTGACTTCA TCATAAGGTA CTTCTTCGTG GTGCTGACGG TGCTGACACT CTTGGGCCTG 
CTCCATGGAC TCCTGCTGCT TCCTGTGCTG CTGTCCATCC TGGGCCCCCC ACCACAGGTG 
GTCCAGATAT ACAAGGAGAG TCCACAGGCC CTAAGCTCCA CAGCCCCACA GAGGGGTGGG 
CTCAGGTGGG GCGTGTCCCC CACCCTGCCC CAGAGTTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC ACCACTACCT GGAGCATACG TCCACCCAGC CTCCGATGAG 
CCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( XM_008764050.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762272.1 Rattus norvegicus patched 2 (Ptch2), transcript variant X1, mRNA.

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CloneID	ORa48810
Clone ID Related Accession (Same CDS sequence)	XM_008764050.2 , NM_001108975.2 , XM_008764050.1
Accession Version	XM_008764050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein patched homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCGGC CACTGTCCCT TGGAGAGCTG CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC AAATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG TGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTAGGACTGG TGGCTTTGGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCATTACACC 
AAAGAGAAGC TGGGGGAGGA GGCTGCCTAC ACCTCCCAGA TGTTGATTCA GACTGCACGC 
CAGGAGGGGG CAAACATCCT CACCCCCGAG GCGCTTGGCT TTCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTACAAGT CTCACTTTAT GGGAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGAATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGTTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA TCCCCAGCAG 
CTCCTAGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG TCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTGGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCGG AGGCCTTCCA GAGCACCTTC 
CTGCTGATGA GCCCCCGTCA GCTGTATGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GTGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCAGCCAAC GCATCCCAGC AGATCCATGC TTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCAGGCGTTC TCGGAAGTCA GTGCCACCCG TGTGGTAGGA 
GGCTATCTGC TCATGCTGGC CTATGCCTGC ATAACAATGC TACGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTCGGTCT TGCTGGGGTG TTGCTCGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACTCA GGTACTGCCC 
TTCTTAGCTC TGGGCATCGG TGTGGATGAC ATCTTCCTGC TGGCACATGC ATTCACAAAG 
GCCCCACCCA ACACTCCTCT CCCAGAGCGC ATGGGTGAGT GTTTGCGACG CACCGGCACC 
AGTGTGGCAC TCACGTCCAT CAACAACATG GTCGCCTTCT TCATGGCTGT TCTGGTTCCC 
ATCCCTGCGC TGCGGGCTTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TTGTCTTCCC CGCTGTCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAG 
CGCCTCGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCTG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAAC TAGGGGACAG AGCCGTACCA GTGGGCATTG CCCACCTGAC TGCCACTGTG 
CAAGCTTTTA CCCACTGCGA AGCCAGCAGC CAGCACGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAAC TTCTGACCCA CTGGGCTCTG AACTCTACAG CCCTGGGGGG 
TCCACACGGG ACCTTCTCGG TCAGGAGGAG GGGACGAGGC CAAAGGCGTC CTGCAGGTCT 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC GCCTTTACTG 
CTCCAGACAC AAGCCAAGGC CCTGGTGTTG CTGTTCTTCG GGGCTCTTTT GGGTCTGAGC 
CTCTATGGAG CCACCTTGGT CCAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTCGGGGC 
ACTAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCTGTGCTC CCCCCACCTG CGGCCCAGGC CCCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGTATCCAGA CCGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCCG CAACTCTTAC CGCAACGGCT CTGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAACCTC TGGATTTCAA CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AACTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTAGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCGG AATGGCTGCA TGACAAGTAC GATACCACTG GAGAGAACCT TCGCATCCCG 
GCAGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTGT TGCATGGACT CCAGAAGACT 
GCAGACTTTG TGGAAGCCAT CGAAGGGGCC CGGGCGGCAT GCACAGAGGC AGGCCAGGCA 
GGGGTGCACG CCTACCCCAG TGGCTCTCCC TTTCTCTTCT GGGAGCAGTA CCTGGGCCTT 
CGGCGCTGCT TCCTCTTGGC GGTCTGCATC TTGCTGCTCT GCACCTTCCT CGTCTGTGCT 
CTGCTCTTAC TCAACCCGTG GACAGCTGGC CTCATAGTGC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGTATCAT GGGATTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTA 
ATCCTCGTGG CCTCTGTAGG CATCGGTGTT GAATTCACGG TTCATGTGGC TCTGGGCTTC 
CTGACCAGCC ATGGTAGCCG GAACCTGCGG GCTGCTCGCG CTCTGGAGCA GACCTTTGCC 
CCAGTGACTG ATGGAGCTGT CTCTACATTG CTGGGTCTGC TCATGCTTGC TGGTTCTAAC 
TTTGACTTCA TCATAAGGTA CTTCTTCGTG GTGCTGACGG TGCTGACACT CTTGGGCCTG 
CTCCATGGAC TCCTGCTGCT TCCTGTGCTG CTGTCCATCC TGGGCCCCCC ACCACAGGTG 
GTCCAGATAT ACAAGGAGAG TCCACAGGCC CTAAGCTCCA CAGCCCCACA GAGGGGTGGG 
CTCAGGTGGG GCGTGTCCCC CACCCTGCCC CAGAGTTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC ACCACTACCT GGAGCATACG TCCACCCAGC CTCCGATGAG 
CCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762272.1
CDS	933..4481
Translation	MVRPLSLGELPPSYTPPARSSAPQILAGSLQAPLWLRAYFQGLLFSLGCRIQKHCGKVLFLGLVALGALALGLRVAVIETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTARQEGANILTPEALGFHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPQQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLDPDDPHCPPSAPNRHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGTARDLQGQLLRAEAFQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILQAFSEVSATRVVGGYLLMLAYACITMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPNTPLPERMGECLRRTGTSVALTSINNMVAFFMAVLVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPTSDPLGSELYSPGGSTRDLLGQEEGTRPKASCRSLLCAHWTLAHFARYQFAPLLLQTQAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPAAQAPRTWLHYYRSWLQGIQTAFDQDWASGRITRNSYRNGSEDGALAYKLLIQTGNAQEPLDFNQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTSHGSRNLRAARALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQIYKESPQALSSTAPQRGGLRWGVSPTLPQSFARVTTSMTVALHPPPLPGAYVHPASDEPT

Target ORF information:

RefSeq Version	XM_008764050.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus patched 2 (Ptch2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764050.2

ORF Insert Sequence:

ATGGTTCGGC CACTGTCCCT TGGAGAGCTG CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC AAATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG TGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTAGGACTGG TGGCTTTGGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCATTACACC 
AAAGAGAAGC TGGGGGAGGA GGCTGCCTAC ACCTCCCAGA TGTTGATTCA GACTGCACGC 
CAGGAGGGGG CAAACATCCT CACCCCCGAG GCGCTTGGCT TTCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTACAAGT CTCACTTTAT GGGAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGAATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGTTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA TCCCCAGCAG 
CTCCTAGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG TCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTGGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCGG AGGCCTTCCA GAGCACCTTC 
CTGCTGATGA GCCCCCGTCA GCTGTATGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GTGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCAGCCAAC GCATCCCAGC AGATCCATGC TTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCAGGCGTTC TCGGAAGTCA GTGCCACCCG TGTGGTAGGA 
GGCTATCTGC TCATGCTGGC CTATGCCTGC ATAACAATGC TACGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTCGGTCT TGCTGGGGTG TTGCTCGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACTCA GGTACTGCCC 
TTCTTAGCTC TGGGCATCGG TGTGGATGAC ATCTTCCTGC TGGCACATGC ATTCACAAAG 
GCCCCACCCA ACACTCCTCT CCCAGAGCGC ATGGGTGAGT GTTTGCGACG CACCGGCACC 
AGTGTGGCAC TCACGTCCAT CAACAACATG GTCGCCTTCT TCATGGCTGT TCTGGTTCCC 
ATCCCTGCGC TGCGGGCTTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TTGTCTTCCC CGCTGTCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAG 
CGCCTCGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCTG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAAC TAGGGGACAG AGCCGTACCA GTGGGCATTG CCCACCTGAC TGCCACTGTG 
CAAGCTTTTA CCCACTGCGA AGCCAGCAGC CAGCACGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAAC TTCTGACCCA CTGGGCTCTG AACTCTACAG CCCTGGGGGG 
TCCACACGGG ACCTTCTCGG TCAGGAGGAG GGGACGAGGC CAAAGGCGTC CTGCAGGTCT 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC GCCTTTACTG 
CTCCAGACAC AAGCCAAGGC CCTGGTGTTG CTGTTCTTCG GGGCTCTTTT GGGTCTGAGC 
CTCTATGGAG CCACCTTGGT CCAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTCGGGGC 
ACTAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCTGTGCTC CCCCCACCTG CGGCCCAGGC CCCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGTATCCAGA CCGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCCG CAACTCTTAC CGCAACGGCT CTGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAACCTC TGGATTTCAA CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AACTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTAGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCGG AATGGCTGCA TGACAAGTAC GATACCACTG GAGAGAACCT TCGCATCCCG 
GCAGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTGT TGCATGGACT CCAGAAGACT 
GCAGACTTTG TGGAAGCCAT CGAAGGGGCC CGGGCGGCAT GCACAGAGGC AGGCCAGGCA 
GGGGTGCACG CCTACCCCAG TGGCTCTCCC TTTCTCTTCT GGGAGCAGTA CCTGGGCCTT 
CGGCGCTGCT TCCTCTTGGC GGTCTGCATC TTGCTGCTCT GCACCTTCCT CGTCTGTGCT 
CTGCTCTTAC TCAACCCGTG GACAGCTGGC CTCATAGTGC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGTATCAT GGGATTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTA 
ATCCTCGTGG CCTCTGTAGG CATCGGTGTT GAATTCACGG TTCATGTGGC TCTGGGCTTC 
CTGACCAGCC ATGGTAGCCG GAACCTGCGG GCTGCTCGCG CTCTGGAGCA GACCTTTGCC 
CCAGTGACTG ATGGAGCTGT CTCTACATTG CTGGGTCTGC TCATGCTTGC TGGTTCTAAC 
TTTGACTTCA TCATAAGGTA CTTCTTCGTG GTGCTGACGG TGCTGACACT CTTGGGCCTG 
CTCCATGGAC TCCTGCTGCT TCCTGTGCTG CTGTCCATCC TGGGCCCCCC ACCACAGGTG 
GTCCAGATAT ACAAGGAGAG TCCACAGGCC CTAAGCTCCA CAGCCCCACA GAGGGGTGGG 
CTCAGGTGGG GCGTGTCCCC CACCCTGCCC CAGAGTTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC ACCACTACCT GGAGCATACG TCCACCCAGC CTCCGATGAG 
CCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( XM_008764050.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762272.1 Rattus norvegicus patched 2 (Ptch2), transcript variant X1, mRNA.

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CloneID	ORa48810
Clone ID Related Accession (Same CDS sequence)	XM_008764050.2 , NM_001108975.2 , XM_008764050.1
Accession Version	XM_008764050.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-08-06
Organism	Rattus norvegicus(Norway rat)
Product	protein patched homolog 2 isoform X1
Comment

ATGGTTCGGC CACTGTCCCT TGGAGAGCTG CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC AAATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG TGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTAGGACTGG TGGCTTTGGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCATTACACC 
AAAGAGAAGC TGGGGGAGGA GGCTGCCTAC ACCTCCCAGA TGTTGATTCA GACTGCACGC 
CAGGAGGGGG CAAACATCCT CACCCCCGAG GCGCTTGGCT TTCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTACAAGT CTCACTTTAT GGGAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGAATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGTTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA TCCCCAGCAG 
CTCCTAGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG TCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTGGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCGG AGGCCTTCCA GAGCACCTTC 
CTGCTGATGA GCCCCCGTCA GCTGTATGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GTGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCAGCCAAC GCATCCCAGC AGATCCATGC TTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCAGGCGTTC TCGGAAGTCA GTGCCACCCG TGTGGTAGGA 
GGCTATCTGC TCATGCTGGC CTATGCCTGC ATAACAATGC TACGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTCGGTCT TGCTGGGGTG TTGCTCGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACTCA GGTACTGCCC 
TTCTTAGCTC TGGGCATCGG TGTGGATGAC ATCTTCCTGC TGGCACATGC ATTCACAAAG 
GCCCCACCCA ACACTCCTCT CCCAGAGCGC ATGGGTGAGT GTTTGCGACG CACCGGCACC 
AGTGTGGCAC TCACGTCCAT CAACAACATG GTCGCCTTCT TCATGGCTGT TCTGGTTCCC 
ATCCCTGCGC TGCGGGCTTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TTGTCTTCCC CGCTGTCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAG 
CGCCTCGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCTG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAAC TAGGGGACAG AGCCGTACCA GTGGGCATTG CCCACCTGAC TGCCACTGTG 
CAAGCTTTTA CCCACTGCGA AGCCAGCAGC CAGCACGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAAC TTCTGACCCA CTGGGCTCTG AACTCTACAG CCCTGGGGGG 
TCCACACGGG ACCTTCTCGG TCAGGAGGAG GGGACGAGGC CAAAGGCGTC CTGCAGGTCT 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC GCCTTTACTG 
CTCCAGACAC AAGCCAAGGC CCTGGTGTTG CTGTTCTTCG GGGCTCTTTT GGGTCTGAGC 
CTCTATGGAG CCACCTTGGT CCAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTCGGGGC 
ACTAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCTGTGCTC CCCCCACCTG CGGCCCAGGC CCCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGTATCCAGA CCGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCCG CAACTCTTAC CGCAACGGCT CTGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAACCTC TGGATTTCAA CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AACTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTAGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCGG AATGGCTGCA TGACAAGTAC GATACCACTG GAGAGAACCT TCGCATCCCG 
GCAGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTGT TGCATGGACT CCAGAAGACT 
GCAGACTTTG TGGAAGCCAT CGAAGGGGCC CGGGCGGCAT GCACAGAGGC AGGCCAGGCA 
GGGGTGCACG CCTACCCCAG TGGCTCTCCC TTTCTCTTCT GGGAGCAGTA CCTGGGCCTT 
CGGCGCTGCT TCCTCTTGGC GGTCTGCATC TTGCTGCTCT GCACCTTCCT CGTCTGTGCT 
CTGCTCTTAC TCAACCCGTG GACAGCTGGC CTCATAGTGC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGTATCAT GGGATTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTA 
ATCCTCGTGG CCTCTGTAGG CATCGGTGTT GAATTCACGG TTCATGTGGC TCTGGGCTTC 
CTGACCAGCC ATGGTAGCCG GAACCTGCGG GCTGCTCGCG CTCTGGAGCA GACCTTTGCC 
CCAGTGACTG ATGGAGCTGT CTCTACATTG CTGGGTCTGC TCATGCTTGC TGGTTCTAAC 
TTTGACTTCA TCATAAGGTA CTTCTTCGTG GTGCTGACGG TGCTGACACT CTTGGGCCTG 
CTCCATGGAC TCCTGCTGCT TCCTGTGCTG CTGTCCATCC TGGGCCCCCC ACCACAGGTG 
GTCCAGATAT ACAAGGAGAG TCCACAGGCC CTAAGCTCCA CAGCCCCACA GAGGGGTGGG 
CTCAGGTGGG GCGTGTCCCC CACCCTGCCC CAGAGTTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC ACCACTACCT GGAGCATACG TCCACCCAGC CTCCGATGAG 
CCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_008762272.1

Translation

MVRPLSLGELPPSYTPPARSSAPQILAGSLQAPLWLRAYFQGLLFSLGCRIQKHCGKVLFLGLVALGALALGLRVAVIETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTARQEGANILTPEALGFHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPQQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLDPDDPHCPPSAPNRHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGTARDLQGQLLRAEAFQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILQAFSEVSATRVVGGYLLMLAYACITMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPNTPLPERMGECLRRTGTSVALTSINNMVAFFMAVLVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPTSDPLGSELYSPGGSTRDLLGQEEGTRPKASCRSLLCAHWTLAHFARYQFAPLLLQTQAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPAAQAPRTWLHYYRSWLQGIQTAFDQDWASGRITRNSYRNGSEDGALAYKLLIQTGNAQEPLDFNQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTSHGSRNLRAARALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQIYKESPQALSSTAPQRGGLRWGVSPTLPQSFARVTTSMTVALHPPPLPGAYVHPASDEPT

Target ORF information:

RefSeq Version	XM_008764050.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus patched 2 (Ptch2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764050.1

ORF Insert Sequence:

ATGGTTCGGC CACTGTCCCT TGGAGAGCTG CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC AAATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG TGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTAGGACTGG TGGCTTTGGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCATTACACC 
AAAGAGAAGC TGGGGGAGGA GGCTGCCTAC ACCTCCCAGA TGTTGATTCA GACTGCACGC 
CAGGAGGGGG CAAACATCCT CACCCCCGAG GCGCTTGGCT TTCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTACAAGT CTCACTTTAT GGGAAGTCCT GGGATTTGAA CAAAATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGAATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGTTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA TCCCCAGCAG 
CTCCTAGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG TCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTGGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCGG AGGCCTTCCA GAGCACCTTC 
CTGCTGATGA GCCCCCGTCA GCTGTATGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GTGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCAGCCAAC GCATCCCAGC AGATCCATGC TTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCAGGCGTTC TCGGAAGTCA GTGCCACCCG TGTGGTAGGA 
GGCTATCTGC TCATGCTGGC CTATGCCTGC ATAACAATGC TACGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTCGGTCT TGCTGGGGTG TTGCTCGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACTCA GGTACTGCCC 
TTCTTAGCTC TGGGCATCGG TGTGGATGAC ATCTTCCTGC TGGCACATGC ATTCACAAAG 
GCCCCACCCA ACACTCCTCT CCCAGAGCGC ATGGGTGAGT GTTTGCGACG CACCGGCACC 
AGTGTGGCAC TCACGTCCAT CAACAACATG GTCGCCTTCT TCATGGCTGT TCTGGTTCCC 
ATCCCTGCGC TGCGGGCTTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TTGTCTTCCC CGCTGTCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAG 
CGCCTCGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCTG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAAC TAGGGGACAG AGCCGTACCA GTGGGCATTG CCCACCTGAC TGCCACTGTG 
CAAGCTTTTA CCCACTGCGA AGCCAGCAGC CAGCACGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAAC TTCTGACCCA CTGGGCTCTG AACTCTACAG CCCTGGGGGG 
TCCACACGGG ACCTTCTCGG TCAGGAGGAG GGGACGAGGC CAAAGGCGTC CTGCAGGTCT 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC GCCTTTACTG 
CTCCAGACAC AAGCCAAGGC CCTGGTGTTG CTGTTCTTCG GGGCTCTTTT GGGTCTGAGC 
CTCTATGGAG CCACCTTGGT CCAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTCGGGGC 
ACTAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCC 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCTGTGCTC CCCCCACCTG CGGCCCAGGC CCCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGTATCCAGA CCGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCCG CAACTCTTAC CGCAACGGCT CTGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAACCTC TGGATTTCAA CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AACTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTAGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCGG AATGGCTGCA TGACAAGTAC GATACCACTG GAGAGAACCT TCGCATCCCG 
GCAGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTGT TGCATGGACT CCAGAAGACT 
GCAGACTTTG TGGAAGCCAT CGAAGGGGCC CGGGCGGCAT GCACAGAGGC AGGCCAGGCA 
GGGGTGCACG CCTACCCCAG TGGCTCTCCC TTTCTCTTCT GGGAGCAGTA CCTGGGCCTT 
CGGCGCTGCT TCCTCTTGGC GGTCTGCATC TTGCTGCTCT GCACCTTCCT CGTCTGTGCT 
CTGCTCTTAC TCAACCCGTG GACAGCTGGC CTCATAGTGC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGTATCAT GGGATTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTA 
ATCCTCGTGG CCTCTGTAGG CATCGGTGTT GAATTCACGG TTCATGTGGC TCTGGGCTTC 
CTGACCAGCC ATGGTAGCCG GAACCTGCGG GCTGCTCGCG CTCTGGAGCA GACCTTTGCC 
CCAGTGACTG ATGGAGCTGT CTCTACATTG CTGGGTCTGC TCATGCTTGC TGGTTCTAAC 
TTTGACTTCA TCATAAGGTA CTTCTTCGTG GTGCTGACGG TGCTGACACT CTTGGGCCTG 
CTCCATGGAC TCCTGCTGCT TCCTGTGCTG CTGTCCATCC TGGGCCCCCC ACCACAGGTG 
GTCCAGATAT ACAAGGAGAG TCCACAGGCC CTAAGCTCCA CAGCCCCACA GAGGGGTGGG 
CTCAGGTGGG GCGTGTCCCC CACCCTGCCC CAGAGTTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC ACCACTACCT GGAGCATACG TCCACCCAGC CTCCGATGAG 
CCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lentivirus-mediated silencing of the PTC1 and PTC2 genes promotes recovery from spinal cord injury by activating the Hedgehog signaling pathway in a rat model.
Experimental & molecular medicine49(12)e412(2017 Dec)
Zhang YD,Zhu ZS,Zhang D,Zhang Z,Ma B,Zhao SC,Xue F

Patched 1 and patched 2 redundancy has a key role in regulating epidermal differentiation.
The Journal of investigative dermatology134(7)1981-1990(2014 Jul)
Adolphe C,Nieuwenhuis E,Villani R,Li ZJ,Kaur P,Hui CC,Wainwright BJ

A missense mutation in PTCH2 underlies dominantly inherited NBCCS in a Chinese family.
Journal of medical genetics45(5)303-8(2008 May)
Fan Z,Li J,Du J,Zhang H,Shen Y,Wang CY,Wang S

Mice with a targeted mutation of patched2 are viable but develop alopecia and epidermal hyperplasia.
Molecular and cellular biology26(17)6609-22(2006 Sep)
Nieuwenhuis E,Motoyama J,Barnfield PC,Yoshikawa Y,Zhang X,Mo R,Crackower MA,Hui CC

Characterization of two patched receptors for the vertebrate hedgehog protein family.
Proceedings of the National Academy of Sciences of the United States of America95(23)13630-4(1998 Nov)
Carpenter D,Stone DM,Brush J,Ryan A,Armanini M,Frantz G,Rosenthal A,de Sauvage FJ

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Ptch2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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