Prpf6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prpf6
Entrez Gene ID	366276
Full Name	pre-mRNA processing factor 6
Synonyms	RGD1307103
General protein information	Preferred Names pre-mRNA processing factor 6 Names pre-mRNA-processing factor 6 PRP6 homolog PRP6 pre-mRNA processing factor 6 homolog PRP6 pre-mRNA splicing factor 6 homolog U5 snRNP-associated 102 kDa protein U5-102 kDa protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q43

mRNA and Protein(s)

mRNA	Protein	Name
NM_001079766.1	NP_001073234.1	pre-mRNA-processing factor 6

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRPF6	NP_036601.2
Rattus norvegicus (Norway rat)	Prpf6	NP_001073234.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005640	XP_315659.1
Oryza sativa (rice)	Os10g0498600	NP_001064969.1
Xenopus tropicalis (tropical clawed frog)	prpf6	NP_001025642.1
Canis lupus familiaris (dog)	PRPF6	XP_534481.3
Mus musculus (house mouse)	Prpf6	NP_598462.1
Gallus gallus (chicken)	PRPF6	XP_417426.2
Magnaporthe oryzae (rice blast fungus)	MGG_07213	XP_003715426.1
Arabidopsis thaliana (thale cress)	EMB2770	NP_192252.1
Pan troglodytes (chimpanzee)	PRPF6	XP_001152053.3
Bos taurus (cattle)	PRPF6	NP_001039840.1
Danio rerio (zebrafish)	prpf6	NP_997820.1
Schizosaccharomyces pombe (fission yeast)	prp1	NP_596086.1
Caenorhabditis elegans (roundworm)	prp-6	NP_507525.1
Drosophila melanogaster (fruit fly)	CG6841	NP_649073.1
Neurospora crassa	NCU04606	XP_958849.1

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, L?hrmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

A missense mutation in PRPF6 causes impairment of pre-mRNA splicing and autosomal-dominant retinitis pigmentosa.
Tanackovic G, Ransijn A, Ayuso C, Harper S, Berson EL, Rivolta C
American journal of human genetics88(5)643-9(2011 May)

The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation.
Tripathi V, Ellis JD, Shen Z, Song DY, Pan Q, Watt AT, Freier SM, Bennett CF, Sharma A, Bubulya PA, Blencowe BJ, Prasanth SG, Prasanth KV
Molecular cell39(6)925-38(2010 Sep)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prpf6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prpf6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10505	NM_001079766.1 Latest version!	Rattus norvegicus pre-mRNA processing factor 6 (Prpf6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prpf6 ( NM_001079766.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001073234.1 Rattus norvegicus pre-mRNA processing factor 6 (Prpf6), mRNA.

Price & Availability↑

CloneID	ORa10505
Clone ID Related Accession (Same CDS sequence)	NM_001079766.1
Accession Version	NM_001079766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	pre-mRNA-processing factor 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC128779.1. On or before Dec 12, 2006 this sequence version replaced XM_345486.3, XM_001055907.1. ##Evidence-Data-START## Transcript exon combination :: BC128779.1, FQ221925.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAACAAGA AGAAGAAGCC GTTCCTGGGC ATGCCCGCGC CGCTGGGCTA CGTACCGGGG 
CTGGGCCGGG GTGCCACAGG CTTCACCACC CGATCAGACA TTGGGCCTGC TCGGGATGCA 
AACGACCCTG TGGACGACCG CCATGCCCCA CCAGGGAAGA GAACTGTGGG GGACCAGATG 
AAGAAAAACC AGGCTGCTGA TGACGATGAT GAGGATCTGA ACGATACCAA CTACGATGAG 
TTTAATGGCT ATGCTGGAAG CCTCTTCTCA AGTGGGCCCT ATGAAAAAGA TGATGAGGAA 
GCAGATGCTA TTTACGCAGC TCTGGATAAG AGGATGGATG AGAGAAGAAA AGAGAGAAGA 
GAGCAAAGGG AAAAAGAAGA GATTGAGAAA TACCGAATGG AACGTCCCAA AATCCAGCAG 
CAGTTTTCAG ACCTTAAGAG GAAGTTGGCA GAGGTCACAG AAGAAGAATG GCTTAGCATC 
CCAGAAGTTG GCGATGCCAG AAACAAACGT CAACGGAACC CACGTTATGA GAAGCTGACC 
CCTGTTCCTG ATAGTTTCTT TGCCAAACAT TTACAGACTG GAGAGAACCA TACTTCCGTG 
GATCCCCGAC AAACTCAATT TGGAGGTCTG AATACTCCCT ACCCAGGAGG CCTGAACACT 
CCATACCCAG GTGGAATGAC ACCAGGATTG ATGACACCTG GAACAGGGGA GCTGGACATG 
AGGAAGATTG GCCAGGCCAG GAATACCCTG ATGGACATGA GGCTTAGCCA GGTATCTGAC 
TCAGTGAGTG GGCAGACTGT TGTGGACCCC AAAGGCTATC TGACGGACCT AAACTCTATG 
ATCCCAACCC ATGGTGGCGA TATAAATGAC ATCAAGAAAG CACGACTACT TCTTAAATCT 
GTTCGAGAAA CAAACCCTCA TCATCCACCA GCCTGGATTG CGTCAGCTCG CCTGGAAGAA 
GTCACTGGAA AGTTACAAGT GGCTCGAAAC CTTATTATGA AAGGGACAGA GATGTGCCCC 
AAGAGTGAAG ATGTCTGGCT AGAAGCAGCT AGGTTACAGC CTGGGGACAC AGCCAAGGCT 
GTGGTGGCAC AAGCTGTCCG TCATCTCCCA CAGTCTGTTA GGATTTACAT TAGAGCAGCA 
GAACTAGAAA CAGACATTCG AGCAAAGAAG CGTGTTCTTC GGAAAGCCCT TGAGCATGTT 
CCAAACTCCG TTCGATTGTG GAAAGCAGCT GTTGAGCTTG AAGAGCCTGA AGATGCTAGA 
ATCATGCTGA GCCGAGCAGT AGAGTGCTGT CCCACCAGTG TAGAGCTCTG GCTTGCTTTG 
GCAAGGCTGG AAACTTATGA AAATGCTCGA AAGGTCTTAA ATAAAGCACG TGAGAACATT 
CCTACAGATC GGCACATCTG GATCACCGCT GCCAAGCTTG AGGAGGCCAA TGGGAACACT 
CAGATGGTGG AGAAGATCAT TGACCGAGCC ATCACGTCTC TTCGGGCCAA TGGTGTAGAG 
ATTAATCGCG AACAGTGGAT CCAGGATGCT GAGGAGTGTG ACAGGGCAGG GAGTGTGGCC 
ACGTGCCAGG CCGTCATGCG TGCTGTGATC GGTATCGGGA TAGAGGAGGA AGATCGCAAA 
CACACCTGGA TGGAAGACGC TGACAGTTGT GTGGCCCACA ATGCCCTGGA GTGTGCACGA 
GCCATATATG CCTATGCCCT ACAAGTCTTC CCCAGCAAGA AAAGTGTGTG GTTACGAGCT 
GCATACTTTG AGAAGAACCA TGGTACCAGG GAGTCTTTGG AAGCGCTCTT GCAGAGGGCT 
GTGGCCCACT GCCCCAAGGC AGAGGTGCTG TGGCTCATGG GTGCCAAGTC GAAGTGGCTG 
GCAGGGGATG TCCCTGCAGC AAGAAGCATC CTGGCCCTGG CCTTTCAGGC CAACCCCAAC 
AGCGAAGAGA TCTGGCTGGC GGCTGTGAAG CTGGAGTCAG AGAACAATGA GTACGAGCGA 
GCCCGGAGGC TGCTGGCCAA GGCCCGGAGC AGCGCACCCA CCGCCCGTGT GTTCATGAAG 
TCTGTGAAGC TGGAGTGGGT GCTGGGGAAC ATCACTGCCG CACAGGAGCT GTGCGAGGAG 
GCTCTGAGGC ACTATGAAGA TTTCCCCAAG CTGTGGATGA TGAAGGGGCA GATTGAGGAG 
CAAGGAGAGC TGATGGAGAG GGCCCGGGAG GCCTACAACC AGGGGTTGAA GAAATGTCCT 
CACTCCACGC CACTGTGGCT CTTGCTTTCC CGGCTAGAGG AAAAGATTGG GCAGCTGACT 
CGTGCTCGGG CCATTTTAGA GAAATCTCGT CTGAAAAACC CAAAGAACCC TGGGCTGTGG 
TTGGAGTCAG TGCGGCTGGA GTACCGGGCA GGGCTGAAGA ATATTGCCAA TACCCTCATG 
GCCAAGGCGC TGCAGGAGTG CCCTAACTCT GGTATCCTGT GGTCTGAAGC TGTCTTCCTT 
GAGGCAAGGC CCCAGAGAAA GACCAAGAGT GTGGATGCCC TGAAGAAGTG TGAACATGAC 
CCCCATGTGC TTTTAGCTGT GGCTAAGCTG TTCTGGAGTG AAAGAAAGAT CACCAAGGCT 
CGGGAGTGGT TCCATCGCAC CGTGAAGATT GATTCAGATC TGGGGGATGC CTGGGCCTTC 
TTCTACAAAT TTGAACTACA GCATGGCACT GAGGAGCAAC AGGAAGAGGT GAGGAAACGC 
TGTGAGAATG CAGAGCCCCG ACATGGAGAG CTGTGGTGTG CTGTGTCCAA GGATATCACC 
AACTGGCAGA GAAAGATTGG GGAAATCCTA GTGCTGGTGG CCGCCCGTAT CAAAAACACC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073234.1
CDS	112..2937
Translation	MNKKKKPFLGMPAPLGYVPGLGRGATGFTTRSDIGPARDANDPVDDRHAPPGKRTVGDQMKKNQAADDDDEDLNDTNYDEFNGYAGSLFSSGPYEKDDEEADAIYAALDKRMDERRKERREQREKEEIEKYRMERPKIQQQFSDLKRKLAEVTEEEWLSIPEVGDARNKRQRNPRYEKLTPVPDSFFAKHLQTGENHTSVDPRQTQFGGLNTPYPGGLNTPYPGGMTPGLMTPGTGELDMRKIGQARNTLMDMRLSQVSDSVSGQTVVDPKGYLTDLNSMIPTHGGDINDIKKARLLLKSVRETNPHHPPAWIASARLEEVTGKLQVARNLIMKGTEMCPKSEDVWLEAARLQPGDTAKAVVAQAVRHLPQSVRIYIRAAELETDIRAKKRVLRKALEHVPNSVRLWKAAVELEEPEDARIMLSRAVECCPTSVELWLALARLETYENARKVLNKARENIPTDRHIWITAAKLEEANGNTQMVEKIIDRAITSLRANGVEINREQWIQDAEECDRAGSVATCQAVMRAVIGIGIEEEDRKHTWMEDADSCVAHNALECARAIYAYALQVFPSKKSVWLRAAYFEKNHGTRESLEALLQRAVAHCPKAEVLWLMGAKSKWLAGDVPAARSILALAFQANPNSEEIWLAAVKLESENNEYERARRLLAKARSSAPTARVFMKSVKLEWVLGNITAAQELCEEALRHYEDFPKLWMMKGQIEEQGELMERAREAYNQGLKKCPHSTPLWLLLSRLEEKIGQLTRARAILEKSRLKNPKNPGLWLESVRLEYRAGLKNIANTLMAKALQECPNSGILWSEAVFLEARPQRKTKSVDALKKCEHDPHVLLAVAKLFWSERKITKAREWFHRTVKIDSDLGDAWAFFYKFELQHGTEEQQEEVRKRCENAEPRHGELWCAVSKDITNWQRKIGEILVLVAARIKNTF

Target ORF information:

RefSeq Version	NM_001079766.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pre-mRNA processing factor 6 (Prpf6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079766.1

ORF Insert Sequence:

ATGAACAAGA AGAAGAAGCC GTTCCTGGGC ATGCCCGCGC CGCTGGGCTA CGTACCGGGG 
CTGGGCCGGG GTGCCACAGG CTTCACCACC CGATCAGACA TTGGGCCTGC TCGGGATGCA 
AACGACCCTG TGGACGACCG CCATGCCCCA CCAGGGAAGA GAACTGTGGG GGACCAGATG 
AAGAAAAACC AGGCTGCTGA TGACGATGAT GAGGATCTGA ACGATACCAA CTACGATGAG 
TTTAATGGCT ATGCTGGAAG CCTCTTCTCA AGTGGGCCCT ATGAAAAAGA TGATGAGGAA 
GCAGATGCTA TTTACGCAGC TCTGGATAAG AGGATGGATG AGAGAAGAAA AGAGAGAAGA 
GAGCAAAGGG AAAAAGAAGA GATTGAGAAA TACCGAATGG AACGTCCCAA AATCCAGCAG 
CAGTTTTCAG ACCTTAAGAG GAAGTTGGCA GAGGTCACAG AAGAAGAATG GCTTAGCATC 
CCAGAAGTTG GCGATGCCAG AAACAAACGT CAACGGAACC CACGTTATGA GAAGCTGACC 
CCTGTTCCTG ATAGTTTCTT TGCCAAACAT TTACAGACTG GAGAGAACCA TACTTCCGTG 
GATCCCCGAC AAACTCAATT TGGAGGTCTG AATACTCCCT ACCCAGGAGG CCTGAACACT 
CCATACCCAG GTGGAATGAC ACCAGGATTG ATGACACCTG GAACAGGGGA GCTGGACATG 
AGGAAGATTG GCCAGGCCAG GAATACCCTG ATGGACATGA GGCTTAGCCA GGTATCTGAC 
TCAGTGAGTG GGCAGACTGT TGTGGACCCC AAAGGCTATC TGACGGACCT AAACTCTATG 
ATCCCAACCC ATGGTGGCGA TATAAATGAC ATCAAGAAAG CACGACTACT TCTTAAATCT 
GTTCGAGAAA CAAACCCTCA TCATCCACCA GCCTGGATTG CGTCAGCTCG CCTGGAAGAA 
GTCACTGGAA AGTTACAAGT GGCTCGAAAC CTTATTATGA AAGGGACAGA GATGTGCCCC 
AAGAGTGAAG ATGTCTGGCT AGAAGCAGCT AGGTTACAGC CTGGGGACAC AGCCAAGGCT 
GTGGTGGCAC AAGCTGTCCG TCATCTCCCA CAGTCTGTTA GGATTTACAT TAGAGCAGCA 
GAACTAGAAA CAGACATTCG AGCAAAGAAG CGTGTTCTTC GGAAAGCCCT TGAGCATGTT 
CCAAACTCCG TTCGATTGTG GAAAGCAGCT GTTGAGCTTG AAGAGCCTGA AGATGCTAGA 
ATCATGCTGA GCCGAGCAGT AGAGTGCTGT CCCACCAGTG TAGAGCTCTG GCTTGCTTTG 
GCAAGGCTGG AAACTTATGA AAATGCTCGA AAGGTCTTAA ATAAAGCACG TGAGAACATT 
CCTACAGATC GGCACATCTG GATCACCGCT GCCAAGCTTG AGGAGGCCAA TGGGAACACT 
CAGATGGTGG AGAAGATCAT TGACCGAGCC ATCACGTCTC TTCGGGCCAA TGGTGTAGAG 
ATTAATCGCG AACAGTGGAT CCAGGATGCT GAGGAGTGTG ACAGGGCAGG GAGTGTGGCC 
ACGTGCCAGG CCGTCATGCG TGCTGTGATC GGTATCGGGA TAGAGGAGGA AGATCGCAAA 
CACACCTGGA TGGAAGACGC TGACAGTTGT GTGGCCCACA ATGCCCTGGA GTGTGCACGA 
GCCATATATG CCTATGCCCT ACAAGTCTTC CCCAGCAAGA AAAGTGTGTG GTTACGAGCT 
GCATACTTTG AGAAGAACCA TGGTACCAGG GAGTCTTTGG AAGCGCTCTT GCAGAGGGCT 
GTGGCCCACT GCCCCAAGGC AGAGGTGCTG TGGCTCATGG GTGCCAAGTC GAAGTGGCTG 
GCAGGGGATG TCCCTGCAGC AAGAAGCATC CTGGCCCTGG CCTTTCAGGC CAACCCCAAC 
AGCGAAGAGA TCTGGCTGGC GGCTGTGAAG CTGGAGTCAG AGAACAATGA GTACGAGCGA 
GCCCGGAGGC TGCTGGCCAA GGCCCGGAGC AGCGCACCCA CCGCCCGTGT GTTCATGAAG 
TCTGTGAAGC TGGAGTGGGT GCTGGGGAAC ATCACTGCCG CACAGGAGCT GTGCGAGGAG 
GCTCTGAGGC ACTATGAAGA TTTCCCCAAG CTGTGGATGA TGAAGGGGCA GATTGAGGAG 
CAAGGAGAGC TGATGGAGAG GGCCCGGGAG GCCTACAACC AGGGGTTGAA GAAATGTCCT 
CACTCCACGC CACTGTGGCT CTTGCTTTCC CGGCTAGAGG AAAAGATTGG GCAGCTGACT 
CGTGCTCGGG CCATTTTAGA GAAATCTCGT CTGAAAAACC CAAAGAACCC TGGGCTGTGG 
TTGGAGTCAG TGCGGCTGGA GTACCGGGCA GGGCTGAAGA ATATTGCCAA TACCCTCATG 
GCCAAGGCGC TGCAGGAGTG CCCTAACTCT GGTATCCTGT GGTCTGAAGC TGTCTTCCTT 
GAGGCAAGGC CCCAGAGAAA GACCAAGAGT GTGGATGCCC TGAAGAAGTG TGAACATGAC 
CCCCATGTGC TTTTAGCTGT GGCTAAGCTG TTCTGGAGTG AAAGAAAGAT CACCAAGGCT 
CGGGAGTGGT TCCATCGCAC CGTGAAGATT GATTCAGATC TGGGGGATGC CTGGGCCTTC 
TTCTACAAAT TTGAACTACA GCATGGCACT GAGGAGCAAC AGGAAGAGGT GAGGAAACGC 
TGTGAGAATG CAGAGCCCCG ACATGGAGAG CTGTGGTGTG CTGTGTCCAA GGATATCACC 
AACTGGCAGA GAAAGATTGG GGAAATCCTA GTGCTGGTGG CCGCCCGTAT CAAAAACACC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,L?hrmann R,Stark H

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

A missense mutation in PRPF6 causes impairment of pre-mRNA splicing and autosomal-dominant retinitis pigmentosa.
American journal of human genetics88(5)643-9(2011 May)
Tanackovic G,Ransijn A,Ayuso C,Harper S,Berson EL,Rivolta C

The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation.
Molecular cell39(6)925-38(2010 Sep)
Tripathi V,Ellis JD,Shen Z,Song DY,Pan Q,Watt AT,Freier SM,Bennett CF,Sharma A,Bubulya PA,Blencowe BJ,Prasanth SG,Prasanth KV

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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Prpf6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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