Ikbke cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ikbke
Entrez Gene ID	363984
Full Name	inhibitor of nuclear factor kappa B kinase subunit epsilon
General protein information	Preferred Names inhibitor of nuclear factor kappa B kinase subunit epsilon Names inhibitor of nuclear factor kappa-B kinase subunit epsilon inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon inhibitor of kappaB kinase epsilon
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q13

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598875.1	XP_017454364.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform X1
NM_001108854.1	NP_001102324.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon

Pathways from BioSystems

Homologies

	IKBKE	XP_001088252.1
Rattus norvegicus (Norway rat)	Ikbke	NP_001102324.1
Homo sapiens (human)	IKBKE	NP_054721.1
Xenopus tropicalis (tropical clawed frog)	ikbke	XP_002940557.2
Canis lupus familiaris (dog)	IKBKE	XP_536108.3
Mus musculus (house mouse)	Ikbke	NP_062751.2
Danio rerio (zebrafish)	ikbke	NP_001002751.1
Bos taurus (cattle)	IKBKE	NP_001039810.1
Gallus gallus (chicken)	IKBKE	XP_004935017.1

Gene Ontology
Bibliography

Related articles in PubMed

The Type I Interferon-IRF7 Axis Mediates Transcriptional Expression of Usp25 Gene.
Ren Y, Zhao Y, Lin D, Xu X, Zhu Q, Yao J, Shu HB, Zhong B
The Journal of biological chemistry291(25)13206-15(2016 Jun)

Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKK? kinase-mediated antiviral response.
Rajsbaum R, Versteeg GA, Schmid S, Maestre AM, Belicha-Villanueva A, Mart?nez-Romero C, Patel JR, Morrison J, Pisanelli G, Miorin L, Laurent-Rolle M, Moulton HM, Stein DA, Fernandez-Sesma A, tenOever BR, Garc?a-Sastre A
Immunity40(6)880-95(2014 Jun)

Molecular cloning, functional characterization and antiviral activity of porcine DDX3X.
Chen Q, Liu Q, Liu D, Wang D, Chen H, Xiao S, Fang L
Biochemical and biophysical research communications443(4)1169-75(2014 Jan)

IKK? knockout prevents high fat diet induced arterial atherosclerosis and NF-?B signaling in mice.
Cao C, Zhu Y, Chen W, Li L, Qi Y, Wang X, Zhao Y, Wan X, Chen X
PloS one8(5)e64930(2013)

SUMOylation-dependent localization of IKKepsilon in PML nuclear bodies is essential for protection against DNA-damage-triggered cell death.
Renner F, Moreno R, Schmitz ML
Molecular cell37(4)503-15(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ikbke gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ikbke cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08501	NM_001108854.1 Latest version!	Rattus norvegicus inhibitor of nuclear factor kappa B kinase subunit epsilon (Ikbke), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa58111	XM_017598875.1 Latest version!	Rattus norvegicus inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (Ikbke), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ikbke ( NM_001108854.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102324.1 Rattus norvegicus inhibitor of nuclear factor kappa B kinase subunit epsilon (Ikbke), mRNA.

Price & Availability↑

CloneID	ORa08501
Clone ID Related Accession (Same CDS sequence)	NM_001108854.1
Accession Version	NM_001108854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473958.2. On or before Oct 4, 2007 this sequence version replaced XM_344139.3, XM_001058036.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCAGAGTA CCACTAACTA CCTGTGGCAT ACTGATGACC TGCTAGGGCA GGGGGCCACT 
GCCAATGTGT ACAAGGCCCG GAATAAGAAA TCCGGGGAGG TGGTTGCTGT AAAGGTCTTC 
AACTCAGCCA GCTACCGGCG GCCCCCTGAG GTACAAGTGA GGGAGTTCGA GGTCTTGCGG 
AGGCTGGATC ATGAGAACAT CGTGAAGCTA TTCGCAGTGG AGGAAACGGG AGGCAGCCGG 
CAGAAGGTGC TAGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGCGT GCTGGAAGAC 
CCTGAGAACA CATTCGGCCT TCCTGAGGAG GAGTTCCTGG TGGTACTGCG CTGTGTGGTG 
GCTGGCATGA ACCACCTGCG GGAGAATGGC ATTGTCCACC GTGACATCAA GCCCGGGAAT 
ATCATGCGCC TGGTAGGCAA GGAGGGGCAG AGCATCTACA AGCTGTCTGA CTTCGGGGCT 
GCCCGGAAGC TGGACGACGA TGAGAAGTTT GTTTCTGTCT ATGGTACAGA GGAGTACCTG 
CACCCTGACA TGTATGAGCG GGCAGTGCTT CGCAAACCCC AGCAAAAGGC GTTCGGGGTG 
ACAGTGGATC TCTGGAGTAT TGGGGTGACC TTGTACCACG CAGCCACCGG TAGTCTGCCC 
TTCATCCCCT TTGGTGGGCC CCGGCGCAAC AAGGAGATCA TGTATAGGAT CACCACAGAG 
AAGCCAGCTG GGGCCATTTC CGGGACTCAG AAGCAGGAAA ATGGTCCCCT GGAATGGAGC 
TACAGCCTCC CCATCACCTG TAGACTGTCT GTGGGGCTGC AGAGTCAGCT AGTGCCCATC 
CTGGCCAACA TCCTGGAGGC CGAACAGGCT AAATGCTGGG GCTTCGATCA GTTCTTTGCA 
GAGACCAGTG ACATCCTGCA GCGAACTGTC ATCCATGTCT TCTCCCTGCC CCAGGCGGTT 
TTGCATCACA TCTACATCCA TGCCCACAAC ACGATTGCCA TCTTTTTGGA GGCTGTATAT 
GAACAGACCA ACGTGGCCCC CAAACACCAA GAATACTTCT TCGAGGGTCA CCCTTGTGTC 
CTCGAGCCAA GCCTCTCAGC CCAGCACATC GCCCACACAG CTGCCAGCAG CCCTCTAACT 
CTGTTCAGCA TGGCCAGCGA CACACCCAAG GGGCTGGCCT TCAGGGACCC TGCTCTGGAT 
GTCCCAAAGT TCGTCCCTAA GGTTGACCTA CAGGCCGATT ACAGCACCGC TAAGGGCGTG 
CTGGGCGCTG GTTACCAGGC CTTGTGGCTG GCGCGGGTCC TGCTGGATGG ACAGGCGTTG 
ATGCTTCGAG GGTTACACTG GGTCCTGGTG GTGCTTCAGA ACACGTGCCA ACAGACACTG 
GAGATCACAC GGACAGCCCT CCTCTTCCTC AGTAGCAGCC TGGGCACTGA AAGGCTCAGC 
AGTGGAGCTG GGATGCTTGA CATCCAGGAA CTAAAGGAAG CCACGGAGCT GAGGTCCAGG 
CTGCAGACTT TCTCAGAGGT CCTGTCTAAG TGTTCCCACA ATGTCACAGA AACCCAAAGG 
AGCCTGAGTT GTCTGGTTGG AGAGCTTTTG AAGAGCCGGG ATCAGATTCA TGAGGATAAC 
AGAAGTATCC AGAAGATTCA GTGTTGTTTG GACAAGATGC ACTTCATCTA CAAACAGTTC 
AAGAAGTCTA GGATGAGGCC AGGCCTCAGC TACAATGAGG AGCAGATCCA CAAGCTGGAT 
AAGGTAAATT TTAGTCACCT AGCCAAGAGG CTGCTGCAGG TGTTCCAGGA GGAGTGTGCA 
CAGACGTATC AGGTGTCACT GGTCACACAT GGCAAGCGGA TGAGGCAGGT GCAGAAGGCC 
CAGAACCACC TGCACCTCAT TGGCCACTCC GTGGCTACCT GTAACTCAGA AGCCCGGGGA 
GCCCAGGAGA GCCTGGCCAA GATGTTTGAT CAGCTCCTCC TGGACAGAGC TTCCAAACAG 
GGAGCTGAGG TCTCACCACA ACCTATGGCA ACTCATCCCG GCCCCGATCC GAAGGACCTG 
GTCTTCCACA TGCAGGAACT TTGTAATGAT ATGAAGCTAT TGGCCTTTGA TCTCCAGGAC 
AACAACAGAC TCATCGAACG GCTACATAGA GTTCCACCAA CACCAGAAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102324.1
CDS	376..2529
Translation	MQSTTNYLWHTDDLLGQGATANVYKARNKKSGEVVAVKVFNSASYRRPPEVQVREFEVLRRLDHENIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLEDPENTFGLPEEEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGKEGQSIYKLSDFGAARKLDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAISGTQKQENGPLEWSYSLPITCRLSVGLQSQLVPILANILEAEQAKCWGFDQFFAETSDILQRTVIHVFSLPQAVLHHIYIHAHNTIAIFLEAVYEQTNVAPKHQEYFFEGHPCVLEPSLSAQHIAHTAASSPLTLFSMASDTPKGLAFRDPALDVPKFVPKVDLQADYSTAKGVLGAGYQALWLARVLLDGQALMLRGLHWVLVVLQNTCQQTLEITRTALLFLSSSLGTERLSSGAGMLDIQELKEATELRSRLQTFSEVLSKCSHNVTETQRSLSCLVGELLKSRDQIHEDNRSIQKIQCCLDKMHFIYKQFKKSRMRPGLSYNEEQIHKLDKVNFSHLAKRLLQVFQEECAQTYQVSLVTHGKRMRQVQKAQNHLHLIGHSVATCNSEARGAQESLAKMFDQLLLDRASKQGAEVSPQPMATHPGPDPKDLVFHMQELCNDMKLLAFDLQDNNRLIERLHRVPPTPEV

Target ORF information:

RefSeq Version	NM_001108854.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus inhibitor of nuclear factor kappa B kinase subunit epsilon (Ikbke), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108854.1

ORF Insert Sequence:

ATGCAGAGTA CCACTAACTA CCTGTGGCAT ACTGATGACC TGCTAGGGCA GGGGGCCACT 
GCCAATGTGT ACAAGGCCCG GAATAAGAAA TCCGGGGAGG TGGTTGCTGT AAAGGTCTTC 
AACTCAGCCA GCTACCGGCG GCCCCCTGAG GTACAAGTGA GGGAGTTCGA GGTCTTGCGG 
AGGCTGGATC ATGAGAACAT CGTGAAGCTA TTCGCAGTGG AGGAAACGGG AGGCAGCCGG 
CAGAAGGTGC TAGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGCGT GCTGGAAGAC 
CCTGAGAACA CATTCGGCCT TCCTGAGGAG GAGTTCCTGG TGGTACTGCG CTGTGTGGTG 
GCTGGCATGA ACCACCTGCG GGAGAATGGC ATTGTCCACC GTGACATCAA GCCCGGGAAT 
ATCATGCGCC TGGTAGGCAA GGAGGGGCAG AGCATCTACA AGCTGTCTGA CTTCGGGGCT 
GCCCGGAAGC TGGACGACGA TGAGAAGTTT GTTTCTGTCT ATGGTACAGA GGAGTACCTG 
CACCCTGACA TGTATGAGCG GGCAGTGCTT CGCAAACCCC AGCAAAAGGC GTTCGGGGTG 
ACAGTGGATC TCTGGAGTAT TGGGGTGACC TTGTACCACG CAGCCACCGG TAGTCTGCCC 
TTCATCCCCT TTGGTGGGCC CCGGCGCAAC AAGGAGATCA TGTATAGGAT CACCACAGAG 
AAGCCAGCTG GGGCCATTTC CGGGACTCAG AAGCAGGAAA ATGGTCCCCT GGAATGGAGC 
TACAGCCTCC CCATCACCTG TAGACTGTCT GTGGGGCTGC AGAGTCAGCT AGTGCCCATC 
CTGGCCAACA TCCTGGAGGC CGAACAGGCT AAATGCTGGG GCTTCGATCA GTTCTTTGCA 
GAGACCAGTG ACATCCTGCA GCGAACTGTC ATCCATGTCT TCTCCCTGCC CCAGGCGGTT 
TTGCATCACA TCTACATCCA TGCCCACAAC ACGATTGCCA TCTTTTTGGA GGCTGTATAT 
GAACAGACCA ACGTGGCCCC CAAACACCAA GAATACTTCT TCGAGGGTCA CCCTTGTGTC 
CTCGAGCCAA GCCTCTCAGC CCAGCACATC GCCCACACAG CTGCCAGCAG CCCTCTAACT 
CTGTTCAGCA TGGCCAGCGA CACACCCAAG GGGCTGGCCT TCAGGGACCC TGCTCTGGAT 
GTCCCAAAGT TCGTCCCTAA GGTTGACCTA CAGGCCGATT ACAGCACCGC TAAGGGCGTG 
CTGGGCGCTG GTTACCAGGC CTTGTGGCTG GCGCGGGTCC TGCTGGATGG ACAGGCGTTG 
ATGCTTCGAG GGTTACACTG GGTCCTGGTG GTGCTTCAGA ACACGTGCCA ACAGACACTG 
GAGATCACAC GGACAGCCCT CCTCTTCCTC AGTAGCAGCC TGGGCACTGA AAGGCTCAGC 
AGTGGAGCTG GGATGCTTGA CATCCAGGAA CTAAAGGAAG CCACGGAGCT GAGGTCCAGG 
CTGCAGACTT TCTCAGAGGT CCTGTCTAAG TGTTCCCACA ATGTCACAGA AACCCAAAGG 
AGCCTGAGTT GTCTGGTTGG AGAGCTTTTG AAGAGCCGGG ATCAGATTCA TGAGGATAAC 
AGAAGTATCC AGAAGATTCA GTGTTGTTTG GACAAGATGC ACTTCATCTA CAAACAGTTC 
AAGAAGTCTA GGATGAGGCC AGGCCTCAGC TACAATGAGG AGCAGATCCA CAAGCTGGAT 
AAGGTAAATT TTAGTCACCT AGCCAAGAGG CTGCTGCAGG TGTTCCAGGA GGAGTGTGCA 
CAGACGTATC AGGTGTCACT GGTCACACAT GGCAAGCGGA TGAGGCAGGT GCAGAAGGCC 
CAGAACCACC TGCACCTCAT TGGCCACTCC GTGGCTACCT GTAACTCAGA AGCCCGGGGA 
GCCCAGGAGA GCCTGGCCAA GATGTTTGAT CAGCTCCTCC TGGACAGAGC TTCCAAACAG 
GGAGCTGAGG TCTCACCACA ACCTATGGCA ACTCATCCCG GCCCCGATCC GAAGGACCTG 
GTCTTCCACA TGCAGGAACT TTGTAATGAT ATGAAGCTAT TGGCCTTTGA TCTCCAGGAC 
AACAACAGAC TCATCGAACG GCTACATAGA GTTCCACCAA CACCAGAAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ikbke ( XM_017598875.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454364.1 Rattus norvegicus inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (Ikbke), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa58111
Clone ID Related Accession (Same CDS sequence)	XM_017598875.1
Accession Version	XM_017598875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTA CCACTAACTA CCTGTGGCAT ACTGATGACC TGCTAGGGCA GGGGGCCACT 
GCCAATGTGT ACAAGGCCCG GAATAAGAAA TCCGGGGAGG TGGTTGCTGT AAAGGTCTTC 
AACTCAGCCA GCTACCGGCG GCCCCCTGAG GTACAAGTGA GGGAGTTCGA GGTCTTGCGG 
AGGCTGGATC ATGAGAACAT CGTGAAGCTA TTCGCAGTGG AGGAAACGGG AGGCAGCCGG 
CAGAAGGTGC TAGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGCGT GCTGGAAGAC 
CCTGAGAACA CATTCGGCCT TCCTGAGGAG GAGTTCCTGG TGGTACTGCG CTGTGTGGTG 
GCTGGCATGA ACCACCTGCG GGAGAATGGC ATTGTCCACC GTGACATCAA GCCCGGGAAT 
ATCATGCGCC TGGTAGGCAA GGAGGGGCAG AGCATCTACA AGCTGTCTGA CTTCGGGGCT 
GCCCGGAAGC TGGACGACGA TGAGAAGTTT GTTTCTGTCT ATGGTACAGA GGAGTACCTG 
CACCCTGACA TGTATGAGCG GGCAGTGCTT CGCAAACCCC AGCAAAAGGC GTTCGGGGTG 
ACAGTGGATC TCTGGAGTAT TGGGGTGACC TTGTACCACG CAGCCACCGG TAGTCTGCCC 
TTCATCCCCT TTGGTGGGCC CCGGCGCAAC AAGGAGATCA TGTATAGGAT CACCACAGAG 
AAGCCAGCTG GGGCCATTTC CGGGACTCAG AAGCAGGAAA ATGGTCCCCT GGAATGGAGC 
TACAGCCTCC CCATCACCTG TAGACTGTCT GTGGGGCTGC AGAGTCAGCT AGTGCCCATC 
CTGGCCAACA TCCTGGAGGC CGAACAGGCT AAATGCTGGG GCTTCGATCA GTTCTTTGCA 
GAGACCAGTG ACATCCTGCA GCGAACTGTC ATCCATGTCT TCTCCCTGCC CCAGGCGGTT 
TTGCATCACA TCTACATCCA TGCCCACAAC ACGATTGCCA TCTTTTTGGA GGCTGTATAT 
GAACAGACCA ACGTGGCCCC CAAACACCAA GAATACTTCT TCGAGGGTCA CCCTTGTGTC 
CTCGAGCCAA GCCTCTCAGC CCAGCACATC GCCCACACAG CTGCCAGCAG CCCTCTAACT 
CTGTTCAGCA TGGCCAGCGA CACACCCAAG GGGCTGGCCT TCAGGGACCC TGCTCTGGAT 
GTCCCAAAGT TCGTCCCTAA GGTTGACCTA CAGGCCGATT ACAGCACCGC TAAGGGCGTG 
CTGGGCGCTG GTTACCAGGC CTTGTGGCTG GCGCGGGTCC TGCTGGATGG ACAGGCGTTG 
ATGCTTCGAG GGTTACACTG GGTCCTGGTG GTGCTTCAGA ACACGTGCCA ACAGACACTG 
GAGATCACAC GGACAGCCCT CCTCTTCCTC AGTAGCAGCC TGGGCACTGA AAGGCTCAGC 
AGTGGAGCTG GGATGCTTGA CATCCAGGAA CTAAAGGAAG CCACGGAGCT GAGGTCCAGG 
CTGCAGACTT TCTCAGAGGT CCTGTCTAAG TGTTCCCACA ATGTCACAGA AACCCAAAGG 
AGCCTGAGTT GTCTGGTTGG AGAGCTTTTG AAGAGCCGGG ATCAGATTCA TGAGGATAAC 
AGAAGTATCC AGAAGATTCA GTGTTGTTTG GACAAGATGC ACTTCATCTA CAAACAGTTC 
AAGAAGTCTA GGATGAGGCC AGGCCTCAGC TACAATGAGG AGCAGATCCA CAAGCTGGAT 
AAGGTAAATT TTAGTCACCT AGCCAAGAGG CTGCTGCAGG TGTTCCAGGA GGAGTGTGCA 
CAGACGTATC AGGTGTCACT GGTCACACAT GGCAAGCGGA TGAGGCAGGT GCAGAAGGCC 
CAGAACCACC TGCACCTCAT TGGCCACTCC GTGGCTACCT GTAACTCAGA AGCCCGGGGA 
GCCCAGGAGA GCCTGGCCAA GATGTTTGAT CAGCTCCTCC TGGACAGAGC TTCCAAACAG 
GGAGCTGAGG TCTCACCACA ACCTATGGCA ACTCATCCCG GCCCCGATCC GAAGGACCTG 
GTCTTCCAGC CATCCCCTGC TCCGTGGCCT CTGCAAGTGG GCTCTGGACC AAGTAACCTG 
GGCCTCAGCC ACCCCGTTCT TGTGCCTTGG ACAGCATGCA GGAACTTTGT AATGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454364.1
CDS	360..2519
Translation	MQSTTNYLWHTDDLLGQGATANVYKARNKKSGEVVAVKVFNSASYRRPPEVQVREFEVLRRLDHENIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLEDPENTFGLPEEEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGKEGQSIYKLSDFGAARKLDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAISGTQKQENGPLEWSYSLPITCRLSVGLQSQLVPILANILEAEQAKCWGFDQFFAETSDILQRTVIHVFSLPQAVLHHIYIHAHNTIAIFLEAVYEQTNVAPKHQEYFFEGHPCVLEPSLSAQHIAHTAASSPLTLFSMASDTPKGLAFRDPALDVPKFVPKVDLQADYSTAKGVLGAGYQALWLARVLLDGQALMLRGLHWVLVVLQNTCQQTLEITRTALLFLSSSLGTERLSSGAGMLDIQELKEATELRSRLQTFSEVLSKCSHNVTETQRSLSCLVGELLKSRDQIHEDNRSIQKIQCCLDKMHFIYKQFKKSRMRPGLSYNEEQIHKLDKVNFSHLAKRLLQVFQEECAQTYQVSLVTHGKRMRQVQKAQNHLHLIGHSVATCNSEARGAQESLAKMFDQLLLDRASKQGAEVSPQPMATHPGPDPKDLVFQPSPAPWPLQVGSGPSNLGLSHPVLVPWTACRNFVMI

Target ORF information:

RefSeq Version	XM_017598875.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (Ikbke), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598875.1

ORF Insert Sequence:

ATGCAGAGTA CCACTAACTA CCTGTGGCAT ACTGATGACC TGCTAGGGCA GGGGGCCACT 
GCCAATGTGT ACAAGGCCCG GAATAAGAAA TCCGGGGAGG TGGTTGCTGT AAAGGTCTTC 
AACTCAGCCA GCTACCGGCG GCCCCCTGAG GTACAAGTGA GGGAGTTCGA GGTCTTGCGG 
AGGCTGGATC ATGAGAACAT CGTGAAGCTA TTCGCAGTGG AGGAAACGGG AGGCAGCCGG 
CAGAAGGTGC TAGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGCGT GCTGGAAGAC 
CCTGAGAACA CATTCGGCCT TCCTGAGGAG GAGTTCCTGG TGGTACTGCG CTGTGTGGTG 
GCTGGCATGA ACCACCTGCG GGAGAATGGC ATTGTCCACC GTGACATCAA GCCCGGGAAT 
ATCATGCGCC TGGTAGGCAA GGAGGGGCAG AGCATCTACA AGCTGTCTGA CTTCGGGGCT 
GCCCGGAAGC TGGACGACGA TGAGAAGTTT GTTTCTGTCT ATGGTACAGA GGAGTACCTG 
CACCCTGACA TGTATGAGCG GGCAGTGCTT CGCAAACCCC AGCAAAAGGC GTTCGGGGTG 
ACAGTGGATC TCTGGAGTAT TGGGGTGACC TTGTACCACG CAGCCACCGG TAGTCTGCCC 
TTCATCCCCT TTGGTGGGCC CCGGCGCAAC AAGGAGATCA TGTATAGGAT CACCACAGAG 
AAGCCAGCTG GGGCCATTTC CGGGACTCAG AAGCAGGAAA ATGGTCCCCT GGAATGGAGC 
TACAGCCTCC CCATCACCTG TAGACTGTCT GTGGGGCTGC AGAGTCAGCT AGTGCCCATC 
CTGGCCAACA TCCTGGAGGC CGAACAGGCT AAATGCTGGG GCTTCGATCA GTTCTTTGCA 
GAGACCAGTG ACATCCTGCA GCGAACTGTC ATCCATGTCT TCTCCCTGCC CCAGGCGGTT 
TTGCATCACA TCTACATCCA TGCCCACAAC ACGATTGCCA TCTTTTTGGA GGCTGTATAT 
GAACAGACCA ACGTGGCCCC CAAACACCAA GAATACTTCT TCGAGGGTCA CCCTTGTGTC 
CTCGAGCCAA GCCTCTCAGC CCAGCACATC GCCCACACAG CTGCCAGCAG CCCTCTAACT 
CTGTTCAGCA TGGCCAGCGA CACACCCAAG GGGCTGGCCT TCAGGGACCC TGCTCTGGAT 
GTCCCAAAGT TCGTCCCTAA GGTTGACCTA CAGGCCGATT ACAGCACCGC TAAGGGCGTG 
CTGGGCGCTG GTTACCAGGC CTTGTGGCTG GCGCGGGTCC TGCTGGATGG ACAGGCGTTG 
ATGCTTCGAG GGTTACACTG GGTCCTGGTG GTGCTTCAGA ACACGTGCCA ACAGACACTG 
GAGATCACAC GGACAGCCCT CCTCTTCCTC AGTAGCAGCC TGGGCACTGA AAGGCTCAGC 
AGTGGAGCTG GGATGCTTGA CATCCAGGAA CTAAAGGAAG CCACGGAGCT GAGGTCCAGG 
CTGCAGACTT TCTCAGAGGT CCTGTCTAAG TGTTCCCACA ATGTCACAGA AACCCAAAGG 
AGCCTGAGTT GTCTGGTTGG AGAGCTTTTG AAGAGCCGGG ATCAGATTCA TGAGGATAAC 
AGAAGTATCC AGAAGATTCA GTGTTGTTTG GACAAGATGC ACTTCATCTA CAAACAGTTC 
AAGAAGTCTA GGATGAGGCC AGGCCTCAGC TACAATGAGG AGCAGATCCA CAAGCTGGAT 
AAGGTAAATT TTAGTCACCT AGCCAAGAGG CTGCTGCAGG TGTTCCAGGA GGAGTGTGCA 
CAGACGTATC AGGTGTCACT GGTCACACAT GGCAAGCGGA TGAGGCAGGT GCAGAAGGCC 
CAGAACCACC TGCACCTCAT TGGCCACTCC GTGGCTACCT GTAACTCAGA AGCCCGGGGA 
GCCCAGGAGA GCCTGGCCAA GATGTTTGAT CAGCTCCTCC TGGACAGAGC TTCCAAACAG 
GGAGCTGAGG TCTCACCACA ACCTATGGCA ACTCATCCCG GCCCCGATCC GAAGGACCTG 
GTCTTCCAGC CATCCCCTGC TCCGTGGCCT CTGCAAGTGG GCTCTGGACC AAGTAACCTG 
GGCCTCAGCC ACCCCGTTCT TGTGCCTTGG ACAGCATGCA GGAACTTTGT AATGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Type I Interferon-IRF7 Axis Mediates Transcriptional Expression of Usp25 Gene.
The Journal of biological chemistry291(25)13206-15(2016 Jun)
Ren Y,Zhao Y,Lin D,Xu X,Zhu Q,Yao J,Shu HB,Zhong B

Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKK? kinase-mediated antiviral response.
Immunity40(6)880-95(2014 Jun)
Rajsbaum R,Versteeg GA,Schmid S,Maestre AM,Belicha-Villanueva A,Mart?nez-Romero C,Patel JR,Morrison J,Pisanelli G,Miorin L,Laurent-Rolle M,Moulton HM,Stein DA,Fernandez-Sesma A,tenOever BR,Garc?a-Sastre A

Molecular cloning, functional characterization and antiviral activity of porcine DDX3X.
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IKK? knockout prevents high fat diet induced arterial atherosclerosis and NF-?B signaling in mice.
PloS one8(5)e64930(2013)
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SUMOylation-dependent localization of IKKepsilon in PML nuclear bodies is essential for protection against DNA-damage-triggered cell death.
Molecular cell37(4)503-15(2010 Feb)
Renner F,Moreno R,Schmitz ML

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Ikbke cDNA ORF clone, Rattus norvegicus(Norway rat)

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