Angptl4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Angptl4
Entrez Gene ID	362850
Full Name	angiopoietin-like 4
General protein information	Preferred Names angiopoietin-like 4 Names angiopoietin-related protein 4 HFARP angiopoietin-like protein 4 hepatic fibrinogen/angiopoietin-related protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q13
Summary	a circulating protein which causes an increase in plasma very low density lipoprotein by inhibition of lipoprotein lipase activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199115.2	NP_954546.1	angiopoietin-related protein 4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ANGPTL4	NP_647475.1
Pan troglodytes (chimpanzee)	ANGPTL4	XP_001147708.3
Canis lupus familiaris (dog)	ANGPTL4	XP_005633282.1
Danio rerio (zebrafish)	angptl4	NP_001243132.1
Bos taurus (cattle)	ANGPTL4	NP_001039508.1
Gallus gallus (chicken)	ANGPTL4	XP_001232284.2
Xenopus tropicalis (tropical clawed frog)	angptl4	XP_002938598.1
Rattus norvegicus (Norway rat)	Angptl4	NP_954546.1
Mus musculus (house mouse)	Angptl4	NP_065606.2

Gene Ontology
Bibliography

Related articles in PubMed

Angiopoietin-Like-4, a Potential Target of Tacrolimus, Predicts Earlier Podocyte Injury in Minimal Change Disease.
Li JS, Chen X, Peng L, Wei SY, Zhao SL, Diao TT, He YX, Liu F, Wei QJ, Zhang QF, Li B
PloS one10(9)e0137049(2015)

Repression of glucocorticoid-stimulated angiopoietin-like 4 gene transcription by insulin.
Kuo T, Chen TC, Yan S, Foo F, Ching C, McQueen A, Wang JC
Journal of lipid research55(5)919-28(2014 May)

Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS.
Wang Y, Chen H, Li H, Zhang J, Gao Y
International journal of molecular medicine32(3)568-76(2013 Sep)

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Bastos-Amador P, Royo F, Gonzalez E, Conde-Vancells J, Palomo-Diez L, Borras FE, Falcon-Perez JM
Journal of proteomics75(12)3574-84(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study represents the first investigation to demonstrate that Angptl4 can predict podocyte injury at earlier stages in minimal change disease
Title: Angiopoietin-Like-4, a Potential Target of Tacrolimus, Predicts Earlier Podocyte Injury in Minimal Change Disease.

GR and FoxO1 are required for Angptl4 transcription activation, and that FoxO1 negatively mediates the suppressive effect of insulin
Title: Repression of glucocorticoid-stimulated angiopoietin-like 4 gene transcription by insulin.

ANGPTL4 protects mesenchymal stem cells from hypoxia/serum deprivation-induced apoptosis by interacting with integrins to stimulate FAK complex, leading to downstream ERK1/2 and PI3K/Akt signaling pathways.
Title: Angiopoietin-like 4 confers resistance to hypoxia/serum deprivation-induced apoptosis through PI3K/Akt and ERK1/2 signaling pathways in mesenchymal stem cells.

the overexpression of Angptl4 exerts protective, anti-inflammatory and anti-angiogenic effects. It re-presents a novel therapeutic target gene for the treatment of acute lung injury induced by LPS.
Title: Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS.

Data suggest that endothelial lipoprotein lipase (LPL) levels in the heart are altered to maintain FA flux and may exploit angiopoietin-like protein-4 (ANGPTL4).
Title: Cardiac lipoprotein lipase activity in the hypertrophied heart may be regulated by fatty acid flux.

The following Angptl4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Angptl4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13521	NM_199115.2 Latest version!	Rattus norvegicus angiopoietin-like 4 (Angptl4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Angptl4 ( NM_199115.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954546.1 Rattus norvegicus angiopoietin-like 4 (Angptl4), mRNA.

Price & Availability↑

CloneID	ORa13521
Clone ID Related Accession (Same CDS sequence)	NM_199115.2
Accession Version	NM_199115.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	angiopoietin-related protein 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078944.1. On Aug 8, 2004 this sequence version replaced NM_199115.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078944.1, AY393999.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCGCTGCG CTCCGACCGC AGGCGCTGCT CTAGTGCTAT GCGCAGCTAC TGCGGGGCTG 
CTGAGCGCGC AAGGGCGCCC TGCACAGCCG GAGCCGCCGC GCTTCGCATC CTGGGATGAA 
ATGAACTTGC TGGCTCACGG GCTGCTGCAG CTCGGTCACG GGCTGCGGGA ACACGTGGAG 
CGCACCCGTG GACAGCTGGG CGCGCTGGAA CGCCGCATGG CTGCCTGCGG TAACGCTTGC 
CAGGGGCCCA AGGGGACAGA CCCGAAGGAT AGAGTCCCCG AAGGCCAGGC TCCTGAGACT 
CTGCAGAGTT TACAGACTCA ACTCAAGGCT CAGAACAGCA AGATCCAGCA ACTGTTCCAG 
AAGGTAGCCC AGCAGCAGAG ATACCTATCA AAGCAGAATC TGAGAATACA GAATCTTCAG 
AGCCAGATTG ACCTCTTGAC CCCCACACAC CTAGACAATG GGGTAGACAA GACTTCGAGG 
GGAAAGAGGC TTCCCAAGAT GGCCCAGCTC ATTGGCTTGA CTCCCAACGC CACCCGCTTA 
CACAGGCCTC CCCGGGACTG CCAGGAACTC TTTCAAGAAG GGGAGCGGCA CAGTGGACTT 
TTCCAGATCC AGCCTCTGGG ATCTCCACCA TTTTTGGTCA ACTGTGAGAT GACTTCAGAT 
GGAGGCTGGA CGGTGATTCA GAGACGCCTG AACGGCTCTG TGGACTTCAA TCAGTCTTGG 
GAAGCCTACA AAGATGGCTT CGGAGATCCC CAAGGCGAGT TCTGGCTGGG CCTAGAGAAG 
ATGCACAGCA TCACAGGGGA CCGAGGAAGC CAGTTGGCTG TGCAGCTCCA GGACTGGGAT 
GGCAATGCCA AATTGCTCCA ATTTCCTATC CATTTGGGGG GTGAGGACAC AGCCTACAGC 
CTGCAGCTCA CCGAGCCCAC GGCCAATGAG CTGGGTGCCA CCAATGTTTC CCCCAATGGC 
CTTTCCCTGC CCTTCTCTAC CTGGGACCAA GACCACGACC TCCGAGGGGA CCTTAACTGT 
GCCAAGAGCC TCTCTGGTGG CTGGTGGTTT GGCACCTGCA GCCATTCCAA TCTAAATGGA 
CAATACTTCC ACTCTATTCC ACGGCAACGG CAGCAGCGTA AAAAGGGGAT CTTCTGGAAA 
ACATGGAAGG GCCGCTACTA TCCACTACAG GCTACCACCC TGTTGATCCA GCCCATGGAG 
GCTACAGCAG CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954546.1
CDS	141..1358
Translation	MRCAPTAGAALVLCAATAGLLSAQGRPAQPEPPRFASWDEMNLLAHGLLQLGHGLREHVERTRGQLGALERRMAACGNACQGPKGTDPKDRVPEGQAPETLQSLQTQLKAQNSKIQQLFQKVAQQQRYLSKQNLRIQNLQSQIDLLTPTHLDNGVDKTSRGKRLPKMAQLIGLTPNATRLHRPPRDCQELFQEGERHSGLFQIQPLGSPPFLVNCEMTSDGGWTVIQRRLNGSVDFNQSWEAYKDGFGDPQGEFWLGLEKMHSITGDRGSQLAVQLQDWDGNAKLLQFPIHLGGEDTAYSLQLTEPTANELGATNVSPNGLSLPFSTWDQDHDLRGDLNCAKSLSGGWWFGTCSHSNLNGQYFHSIPRQRQQRKKGIFWKTWKGRYYPLQATTLLIQPMEATAAS

Target ORF information:

RefSeq Version	NM_199115.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus angiopoietin-like 4 (Angptl4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199115.2

ORF Insert Sequence:

ATGCGCTGCG CTCCGACCGC AGGCGCTGCT CTAGTGCTAT GCGCAGCTAC TGCGGGGCTG 
CTGAGCGCGC AAGGGCGCCC TGCACAGCCG GAGCCGCCGC GCTTCGCATC CTGGGATGAA 
ATGAACTTGC TGGCTCACGG GCTGCTGCAG CTCGGTCACG GGCTGCGGGA ACACGTGGAG 
CGCACCCGTG GACAGCTGGG CGCGCTGGAA CGCCGCATGG CTGCCTGCGG TAACGCTTGC 
CAGGGGCCCA AGGGGACAGA CCCGAAGGAT AGAGTCCCCG AAGGCCAGGC TCCTGAGACT 
CTGCAGAGTT TACAGACTCA ACTCAAGGCT CAGAACAGCA AGATCCAGCA ACTGTTCCAG 
AAGGTAGCCC AGCAGCAGAG ATACCTATCA AAGCAGAATC TGAGAATACA GAATCTTCAG 
AGCCAGATTG ACCTCTTGAC CCCCACACAC CTAGACAATG GGGTAGACAA GACTTCGAGG 
GGAAAGAGGC TTCCCAAGAT GGCCCAGCTC ATTGGCTTGA CTCCCAACGC CACCCGCTTA 
CACAGGCCTC CCCGGGACTG CCAGGAACTC TTTCAAGAAG GGGAGCGGCA CAGTGGACTT 
TTCCAGATCC AGCCTCTGGG ATCTCCACCA TTTTTGGTCA ACTGTGAGAT GACTTCAGAT 
GGAGGCTGGA CGGTGATTCA GAGACGCCTG AACGGCTCTG TGGACTTCAA TCAGTCTTGG 
GAAGCCTACA AAGATGGCTT CGGAGATCCC CAAGGCGAGT TCTGGCTGGG CCTAGAGAAG 
ATGCACAGCA TCACAGGGGA CCGAGGAAGC CAGTTGGCTG TGCAGCTCCA GGACTGGGAT 
GGCAATGCCA AATTGCTCCA ATTTCCTATC CATTTGGGGG GTGAGGACAC AGCCTACAGC 
CTGCAGCTCA CCGAGCCCAC GGCCAATGAG CTGGGTGCCA CCAATGTTTC CCCCAATGGC 
CTTTCCCTGC CCTTCTCTAC CTGGGACCAA GACCACGACC TCCGAGGGGA CCTTAACTGT 
GCCAAGAGCC TCTCTGGTGG CTGGTGGTTT GGCACCTGCA GCCATTCCAA TCTAAATGGA 
CAATACTTCC ACTCTATTCC ACGGCAACGG CAGCAGCGTA AAAAGGGGAT CTTCTGGAAA 
ACATGGAAGG GCCGCTACTA TCCACTACAG GCTACCACCC TGTTGATCCA GCCCATGGAG 
GCTACAGCAG CCTCCTAG