Brd4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brd4
Entrez Gene ID	362844
Full Name	bromodomain containing 4
General protein information	Preferred Names bromodomain containing 4 Names bromodomain-containing protein 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594916.1	XP_017450405.1	bromodomain-containing protein 4 isoform X3
NM_001100903.1	NP_001094373.1	bromodomain-containing protein 4
XM_008765170.2	XP_008763392.1	bromodomain-containing protein 4 isoform X4
XM_008765173.2	XP_008763395.1	bromodomain-containing protein 4 isoform X5
XM_006241072.3	XP_006241134.1	bromodomain-containing protein 4 isoform X1
XM_008765171.2	XP_008763393.2	bromodomain-containing protein 4 isoform X2
XM_006241073.3	XP_006241135.1	bromodomain-containing protein 4 isoform X1
XM_006241074.3	XP_006241136.1	bromodomain-containing protein 4 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Brd4	NP_001094373.1
Danio rerio (zebrafish)	brd4	NP_001104751.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of BRD4 Attenuates Cardiomyocyte Apoptosis via NF-?B pathway in a Rat Model of Myocardial Infarction.
Sun Y, Xie Y, Du L, Sun J, Liu Z
Cardiovascular therapeutics(2018 Jan)

Structural Mechanism of the Oxygenase JMJD6 Recognition by the Extraterminal (ET) Domain of BRD4.
Konuma T, Yu D, Zhao C, Ju Y, Sharma R, Ren C, Zhang Q, Zhou MM, Zeng L
Scientific reports7(1)16272(2017 Nov)

Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-?-mediated Nox4 expression.
Zhou B, Mu J, Gong Y, Lu C, Zhao Y, He T, Qin Z
Redox biology11390-402(2017 04)

Inhibition of BRD4 suppresses tumor growth and enhances iodine uptake in thyroid cancer.
Gao X, Wu X, Zhang X, Hua W, Zhang Y, Maimaiti Y, Gao Z, Zhang Y
Biochemical and biophysical research communications469(3)679-85(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-beta-mediated Nox4 expression.
Title: Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-?-mediated Nox4 expression.

The following Brd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00393	NM_001100903.1 Latest version!	Rattus norvegicus bromodomain containing 4 (Brd4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa00393	XM_006241074.3 Latest version!	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa00393	XM_006241072.3 Latest version!	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa00393	XM_006241073.3 Latest version!	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa29964	XM_008765173.2 Latest version!	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa48379	XM_008765170.2 Latest version!	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62043	XM_008765171.2 Latest version!	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62044	XM_017594916.1 Latest version!	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brd4 ( NM_001100903.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094373.1 Rattus norvegicus bromodomain containing 4 (Brd4), mRNA.

Price & Availability↑

CloneID	ORa00393
Clone ID Related Accession (Same CDS sequence)	NM_001100903.1 , XM_006241072.3 , XM_006241073.3 , XM_006241074.3
Accession Version	NM_001100903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-03
Organism	Rattus norvegicus(Norway rat)
Product	bromodomain-containing protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07055865.1 and AABR07055864.1. On or before Nov 21, 2008 this sequence version replaced XM_343175.3, XM_001066148.1, XM_001065925.1, XM_001077931.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGTAAAGG GCAGGGCTGA GCCACAGCCC CCAGGGCCAG TCATGGGCCG AGGCCAGGGA 
TGCCCACCTG CCTCACCGGC TGCCGTACCT ATGCTGTCCC AGGAGCTGCG CCCACCGTCA 
GTCGTCCAGC CCCAGCCCCT GGTGGTAGTG AAGGAGGAGA AGATTCACTC ACCAATCATC 
CGCAGTGAGC CTTTTAGCAC CTCACTTCGC CCAGAGCCGC CCAAGCACCC AGAGAACATG 
AAGGCCCCAG TCCACCTGCC TCAGCGGCCT GAGATGAAGC CTGTAGATGT AGGGAGGCCT 
GTGATCCGGC CTCCAGAGCA GAGTGCACCC CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CAAAAACACC AGTGGCGCCC AAAAAGGACC TGAAAATTAA GAACATGGGC 
TCCTGGGCCA GCCTGGTACA GAAGCATCCG ACCACCCCAT CCTCCACAGC CAAGTCCTCA 
AGCGACAGCT TTGAGCATTT CCGCCGCGCT GCTCGGGAGA AGGAGGAGCG GGAAAAGGCC 
CTGAAGGCTC AGGCTGAGCA CGCAGAGAAG GAGAAGGAGC GGCTGAGGCA GGAGCGCATG 
AGGAGCCGAG AGGATGAGGA TGCGCTGGAA CAGGCACGTC GAGCCCATGA GGAGGCACGA 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCAG AGACAGGAGC AGCAGCAGCA GCAGCAACAG 
CAGGCAGCTG CTGTGGCCGC TGCCTCCGCC CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT 
ATGCTGGACC AGCAGAGGGA GTTGGCCCGA AAGCGAGAAC AGGAGCGGAG GCGCAGGGAG 
GCAATGGCAG CTACAATTGA CATGAATTTC CAGAGTGATC TTTTGTCAAT ATTTGAAGAA 
AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094373.1
CDS	148..4359
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQSANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTSPQTQMPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEMEENKKSKAKELPPKKTKKNNSSNSNVSKKEPAPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPTGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGRGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENMKAPVHLPQRPEMKPVDVGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQRQEQQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	NM_001100903.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bromodomain containing 4 (Brd4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100903.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGTAAAGG GCAGGGCTGA GCCACAGCCC CCAGGGCCAG TCATGGGCCG AGGCCAGGGA 
TGCCCACCTG CCTCACCGGC TGCCGTACCT ATGCTGTCCC AGGAGCTGCG CCCACCGTCA 
GTCGTCCAGC CCCAGCCCCT GGTGGTAGTG AAGGAGGAGA AGATTCACTC ACCAATCATC 
CGCAGTGAGC CTTTTAGCAC CTCACTTCGC CCAGAGCCGC CCAAGCACCC AGAGAACATG 
AAGGCCCCAG TCCACCTGCC TCAGCGGCCT GAGATGAAGC CTGTAGATGT AGGGAGGCCT 
GTGATCCGGC CTCCAGAGCA GAGTGCACCC CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CAAAAACACC AGTGGCGCCC AAAAAGGACC TGAAAATTAA GAACATGGGC 
TCCTGGGCCA GCCTGGTACA GAAGCATCCG ACCACCCCAT CCTCCACAGC CAAGTCCTCA 
AGCGACAGCT TTGAGCATTT CCGCCGCGCT GCTCGGGAGA AGGAGGAGCG GGAAAAGGCC 
CTGAAGGCTC AGGCTGAGCA CGCAGAGAAG GAGAAGGAGC GGCTGAGGCA GGAGCGCATG 
AGGAGCCGAG AGGATGAGGA TGCGCTGGAA CAGGCACGTC GAGCCCATGA GGAGGCACGA 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCAG AGACAGGAGC AGCAGCAGCA GCAGCAACAG 
CAGGCAGCTG CTGTGGCCGC TGCCTCCGCC CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT 
ATGCTGGACC AGCAGAGGGA GTTGGCCCGA AAGCGAGAAC AGGAGCGGAG GCGCAGGGAG 
GCAATGGCAG CTACAATTGA CATGAATTTC CAGAGTGATC TTTTGTCAAT ATTTGAAGAA 
AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006241072.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241134.1 Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa00393
Clone ID Related Accession (Same CDS sequence)	NM_001100903.1 , XM_006241072.3 , XM_006241073.3 , XM_006241074.3
Accession Version	XM_006241072.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGTAAAGG GCAGGGCTGA GCCACAGCCC CCAGGGCCAG TCATGGGCCG AGGCCAGGGA 
TGCCCACCTG CCTCACCGGC TGCCGTACCT ATGCTGTCCC AGGAGCTGCG CCCACCGTCA 
GTCGTCCAGC CCCAGCCCCT GGTGGTAGTG AAGGAGGAGA AGATTCACTC ACCAATCATC 
CGCAGTGAGC CTTTTAGCAC CTCACTTCGC CCAGAGCCGC CCAAGCACCC AGAGAACATG 
AAGGCCCCAG TCCACCTGCC TCAGCGGCCT GAGATGAAGC CTGTAGATGT AGGGAGGCCT 
GTGATCCGGC CTCCAGAGCA GAGTGCACCC CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CAAAAACACC AGTGGCGCCC AAAAAGGACC TGAAAATTAA GAACATGGGC 
TCCTGGGCCA GCCTGGTACA GAAGCATCCG ACCACCCCAT CCTCCACAGC CAAGTCCTCA 
AGCGACAGCT TTGAGCATTT CCGCCGCGCT GCTCGGGAGA AGGAGGAGCG GGAAAAGGCC 
CTGAAGGCTC AGGCTGAGCA CGCAGAGAAG GAGAAGGAGC GGCTGAGGCA GGAGCGCATG 
AGGAGCCGAG AGGATGAGGA TGCGCTGGAA CAGGCACGTC GAGCCCATGA GGAGGCACGA 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCAG AGACAGGAGC AGCAGCAGCA GCAGCAACAG 
CAGGCAGCTG CTGTGGCCGC TGCCTCCGCC CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT 
ATGCTGGACC AGCAGAGGGA GTTGGCCCGA AAGCGAGAAC AGGAGCGGAG GCGCAGGGAG 
GCAATGGCAG CTACAATTGA CATGAATTTC CAGAGTGATC TTTTGTCAAT ATTTGAAGAA 
AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241134.1
CDS	109..4320
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQSANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTSPQTQMPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEMEENKKSKAKELPPKKTKKNNSSNSNVSKKEPAPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPTGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGRGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENMKAPVHLPQRPEMKPVDVGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQRQEQQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006241072.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241072.3

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGTAAAGG GCAGGGCTGA GCCACAGCCC CCAGGGCCAG TCATGGGCCG AGGCCAGGGA 
TGCCCACCTG CCTCACCGGC TGCCGTACCT ATGCTGTCCC AGGAGCTGCG CCCACCGTCA 
GTCGTCCAGC CCCAGCCCCT GGTGGTAGTG AAGGAGGAGA AGATTCACTC ACCAATCATC 
CGCAGTGAGC CTTTTAGCAC CTCACTTCGC CCAGAGCCGC CCAAGCACCC AGAGAACATG 
AAGGCCCCAG TCCACCTGCC TCAGCGGCCT GAGATGAAGC CTGTAGATGT AGGGAGGCCT 
GTGATCCGGC CTCCAGAGCA GAGTGCACCC CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CAAAAACACC AGTGGCGCCC AAAAAGGACC TGAAAATTAA GAACATGGGC 
TCCTGGGCCA GCCTGGTACA GAAGCATCCG ACCACCCCAT CCTCCACAGC CAAGTCCTCA 
AGCGACAGCT TTGAGCATTT CCGCCGCGCT GCTCGGGAGA AGGAGGAGCG GGAAAAGGCC 
CTGAAGGCTC AGGCTGAGCA CGCAGAGAAG GAGAAGGAGC GGCTGAGGCA GGAGCGCATG 
AGGAGCCGAG AGGATGAGGA TGCGCTGGAA CAGGCACGTC GAGCCCATGA GGAGGCACGA 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCAG AGACAGGAGC AGCAGCAGCA GCAGCAACAG 
CAGGCAGCTG CTGTGGCCGC TGCCTCCGCC CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT 
ATGCTGGACC AGCAGAGGGA GTTGGCCCGA AAGCGAGAAC AGGAGCGGAG GCGCAGGGAG 
GCAATGGCAG CTACAATTGA CATGAATTTC CAGAGTGATC TTTTGTCAAT ATTTGAAGAA 
AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006241073.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241135.1 Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X5, mRNA.

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CloneID	ORa00393
Clone ID Related Accession (Same CDS sequence)	NM_001100903.1 , XM_006241072.3 , XM_006241073.3 , XM_006241074.3
Accession Version	XM_006241073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGTAAAGG GCAGGGCTGA GCCACAGCCC CCAGGGCCAG TCATGGGCCG AGGCCAGGGA 
TGCCCACCTG CCTCACCGGC TGCCGTACCT ATGCTGTCCC AGGAGCTGCG CCCACCGTCA 
GTCGTCCAGC CCCAGCCCCT GGTGGTAGTG AAGGAGGAGA AGATTCACTC ACCAATCATC 
CGCAGTGAGC CTTTTAGCAC CTCACTTCGC CCAGAGCCGC CCAAGCACCC AGAGAACATG 
AAGGCCCCAG TCCACCTGCC TCAGCGGCCT GAGATGAAGC CTGTAGATGT AGGGAGGCCT 
GTGATCCGGC CTCCAGAGCA GAGTGCACCC CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CAAAAACACC AGTGGCGCCC AAAAAGGACC TGAAAATTAA GAACATGGGC 
TCCTGGGCCA GCCTGGTACA GAAGCATCCG ACCACCCCAT CCTCCACAGC CAAGTCCTCA 
AGCGACAGCT TTGAGCATTT CCGCCGCGCT GCTCGGGAGA AGGAGGAGCG GGAAAAGGCC 
CTGAAGGCTC AGGCTGAGCA CGCAGAGAAG GAGAAGGAGC GGCTGAGGCA GGAGCGCATG 
AGGAGCCGAG AGGATGAGGA TGCGCTGGAA CAGGCACGTC GAGCCCATGA GGAGGCACGA 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCAG AGACAGGAGC AGCAGCAGCA GCAGCAACAG 
CAGGCAGCTG CTGTGGCCGC TGCCTCCGCC CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT 
ATGCTGGACC AGCAGAGGGA GTTGGCCCGA AAGCGAGAAC AGGAGCGGAG GCGCAGGGAG 
GCAATGGCAG CTACAATTGA CATGAATTTC CAGAGTGATC TTTTGTCAAT ATTTGAAGAA 
AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241135.1
CDS	287..4498
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQSANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTSPQTQMPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEMEENKKSKAKELPPKKTKKNNSSNSNVSKKEPAPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPTGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGRGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENMKAPVHLPQRPEMKPVDVGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQRQEQQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006241073.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241073.3

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGTAAAGG GCAGGGCTGA GCCACAGCCC CCAGGGCCAG TCATGGGCCG AGGCCAGGGA 
TGCCCACCTG CCTCACCGGC TGCCGTACCT ATGCTGTCCC AGGAGCTGCG CCCACCGTCA 
GTCGTCCAGC CCCAGCCCCT GGTGGTAGTG AAGGAGGAGA AGATTCACTC ACCAATCATC 
CGCAGTGAGC CTTTTAGCAC CTCACTTCGC CCAGAGCCGC CCAAGCACCC AGAGAACATG 
AAGGCCCCAG TCCACCTGCC TCAGCGGCCT GAGATGAAGC CTGTAGATGT AGGGAGGCCT 
GTGATCCGGC CTCCAGAGCA GAGTGCACCC CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CAAAAACACC AGTGGCGCCC AAAAAGGACC TGAAAATTAA GAACATGGGC 
TCCTGGGCCA GCCTGGTACA GAAGCATCCG ACCACCCCAT CCTCCACAGC CAAGTCCTCA 
AGCGACAGCT TTGAGCATTT CCGCCGCGCT GCTCGGGAGA AGGAGGAGCG GGAAAAGGCC 
CTGAAGGCTC AGGCTGAGCA CGCAGAGAAG GAGAAGGAGC GGCTGAGGCA GGAGCGCATG 
AGGAGCCGAG AGGATGAGGA TGCGCTGGAA CAGGCACGTC GAGCCCATGA GGAGGCACGA 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCAG AGACAGGAGC AGCAGCAGCA GCAGCAACAG 
CAGGCAGCTG CTGTGGCCGC TGCCTCCGCC CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT 
ATGCTGGACC AGCAGAGGGA GTTGGCCCGA AAGCGAGAAC AGGAGCGGAG GCGCAGGGAG 
GCAATGGCAG CTACAATTGA CATGAATTTC CAGAGTGATC TTTTGTCAAT ATTTGAAGAA 
AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006241074.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241136.1 Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X6, mRNA.

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CloneID	ORa00393
Clone ID Related Accession (Same CDS sequence)	NM_001100903.1 , XM_006241072.3 , XM_006241073.3 , XM_006241074.3
Accession Version	XM_006241074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGTAAAGG GCAGGGCTGA GCCACAGCCC CCAGGGCCAG TCATGGGCCG AGGCCAGGGA 
TGCCCACCTG CCTCACCGGC TGCCGTACCT ATGCTGTCCC AGGAGCTGCG CCCACCGTCA 
GTCGTCCAGC CCCAGCCCCT GGTGGTAGTG AAGGAGGAGA AGATTCACTC ACCAATCATC 
CGCAGTGAGC CTTTTAGCAC CTCACTTCGC CCAGAGCCGC CCAAGCACCC AGAGAACATG 
AAGGCCCCAG TCCACCTGCC TCAGCGGCCT GAGATGAAGC CTGTAGATGT AGGGAGGCCT 
GTGATCCGGC CTCCAGAGCA GAGTGCACCC CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CAAAAACACC AGTGGCGCCC AAAAAGGACC TGAAAATTAA GAACATGGGC 
TCCTGGGCCA GCCTGGTACA GAAGCATCCG ACCACCCCAT CCTCCACAGC CAAGTCCTCA 
AGCGACAGCT TTGAGCATTT CCGCCGCGCT GCTCGGGAGA AGGAGGAGCG GGAAAAGGCC 
CTGAAGGCTC AGGCTGAGCA CGCAGAGAAG GAGAAGGAGC GGCTGAGGCA GGAGCGCATG 
AGGAGCCGAG AGGATGAGGA TGCGCTGGAA CAGGCACGTC GAGCCCATGA GGAGGCACGA 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCAG AGACAGGAGC AGCAGCAGCA GCAGCAACAG 
CAGGCAGCTG CTGTGGCCGC TGCCTCCGCC CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT 
ATGCTGGACC AGCAGAGGGA GTTGGCCCGA AAGCGAGAAC AGGAGCGGAG GCGCAGGGAG 
GCAATGGCAG CTACAATTGA CATGAATTTC CAGAGTGATC TTTTGTCAAT ATTTGAAGAA 
AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241136.1
CDS	1079..5290
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQSANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTSPQTQMPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEMEENKKSKAKELPPKKTKKNNSSNSNVSKKEPAPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPTGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGRGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENMKAPVHLPQRPEMKPVDVGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQRQEQQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006241074.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241074.3

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGTAAAGG GCAGGGCTGA GCCACAGCCC CCAGGGCCAG TCATGGGCCG AGGCCAGGGA 
TGCCCACCTG CCTCACCGGC TGCCGTACCT ATGCTGTCCC AGGAGCTGCG CCCACCGTCA 
GTCGTCCAGC CCCAGCCCCT GGTGGTAGTG AAGGAGGAGA AGATTCACTC ACCAATCATC 
CGCAGTGAGC CTTTTAGCAC CTCACTTCGC CCAGAGCCGC CCAAGCACCC AGAGAACATG 
AAGGCCCCAG TCCACCTGCC TCAGCGGCCT GAGATGAAGC CTGTAGATGT AGGGAGGCCT 
GTGATCCGGC CTCCAGAGCA GAGTGCACCC CCACCAGGGG CCCCTGACAA GGACAAACAG 
AAACAGGAGC CAAAAACACC AGTGGCGCCC AAAAAGGACC TGAAAATTAA GAACATGGGC 
TCCTGGGCCA GCCTGGTACA GAAGCATCCG ACCACCCCAT CCTCCACAGC CAAGTCCTCA 
AGCGACAGCT TTGAGCATTT CCGCCGCGCT GCTCGGGAGA AGGAGGAGCG GGAAAAGGCC 
CTGAAGGCTC AGGCTGAGCA CGCAGAGAAG GAGAAGGAGC GGCTGAGGCA GGAGCGCATG 
AGGAGCCGAG AGGATGAGGA TGCGCTGGAA CAGGCACGTC GAGCCCATGA GGAGGCACGA 
CGGCGCCAGG AGCAGCAGCA GCAGCAGCAG AGACAGGAGC AGCAGCAGCA GCAGCAACAG 
CAGGCAGCTG CTGTGGCCGC TGCCTCCGCC CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT 
ATGCTGGACC AGCAGAGGGA GTTGGCCCGA AAGCGAGAAC AGGAGCGGAG GCGCAGGGAG 
GCAATGGCAG CTACAATTGA CATGAATTTC CAGAGTGATC TTTTGTCAAT ATTTGAAGAA 
AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_008765173.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763395.1 Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X7, mRNA.

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CloneID	ORa29964
Clone ID Related Accession (Same CDS sequence)	XM_008765173.2
Accession Version	XM_008765173.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bromodomain-containing protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAGAAAATCA ATGAACTGCC TACAGAAGAA 
ACCGAGATCA TGATAGTCCA GGCAAAGGGA CGAGGACGAG GGAGGAAAGA AACAGGGACA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGATGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC CCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCTCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCTCCCAT CATTGCGACC ACCCCACAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCC ACCACCATCG ACCCCATTCA TGAGCCGCCC 
TCACTGGCCC CGGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCCC CAAAGAAGGA TGTACCAGAC TCCCAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT GAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCTAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCCGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT TAAACACCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGGG AGTACAGAGA TGCCCAGGAG TTTGGTGCTG ATGTCCGATT GATGTTTTCC 
AACTGCTACA AGTACAATCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACG 
GTGTCCTCCC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGTG ATAGTTCCTC TGACAGTGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAGCT CCAGGAGCAG CTCAAAGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAACAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAATG GAGGAAAATA AAAAAAGCAA AGCCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGCACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAAGA GGACAAGTGT 
AAGCCTATGT CTTATGAGGA GAAGCGACAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCGAGGG AACCATCACT TAAAAACTCC 
AACCCTGATG AGATTGAGAT TGATTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAT 
GTGATTGCTG GTTCTTCCAA GATGAAGGGC TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCTAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCACCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCGCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCTC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC CCCCACCCCC ACCACCTCCG 
CCCTCCATGC CACAGCAGAC TGCCCCAGCG ATGAAGTCCT CGCCCCCACC CTTCATCACT 
GCCCAGGTAC CCGTCCTGGA ACCACAGCTA CCAGGCAGTG TCTTTGACCC TATTGGCCAC 
TTCACCCAGC CCATCTTGCA CCTGCCGCAG CCAGAGCTGC CTCCTCACCT GCCCCAGCCA 
CCGGAGCACA GCACTCCACC CCATCTCAAC CAGCATGCTG TGGTCTCTCC TCCAGCTTTG 
CACAATGCGC TGCCCCAACA ACCATCTCGG CCCAGCAACC GAGCTGCTGC TCTGCCCCCA 
AAGCCTGCCC GACCCCCAGC TGTGTCCCCT GCCTTGGCCC AGCCCCCCCT GCTCCCACAG 
CCACCTATGG CTCAGCCCCC CCAAGTGCTG CTGGAGGATG AAGAGCCGCC TGCCCCACCC 
CTCACCTCCA TGCAGATGCA GTTGTACCTA CAGCAGCTGC AGAAGGTGCA GCCTCCCACA 
CCACTACTCC CTTCCGTGAA GGTGCAGTCC CAACCCCCAC CACCTTTGCC GCCTCCACCC 
CACCCTTCTG TGCAGCAACA GCAGCTCCAG CAGCAGCCAC CACCACCCCC ACCTCCTCAG 
CCACAGCCAC CACCCCAGCA ACAACACCAG CCCCCACCAC GACCAGTTCA CTTGCCATCC 
ATGCCCTTCT CTGCTCACAT TCAGCAGCCC CCACCACCCA CAGGACAGCA ACCCACCCAC 
CCACCCCCAG GACAGCAGCC ACCCCCACCA CAGCCTGCCA AGCCTCAGCA AGTCATCCAG 
CATCACCCTT CACCCCGGCA CCACAAGTCA GACCCCTACT CAGCTGGTCA TCTTCGTGAA 
GCCCCTTCTC CGCTTATGAT ACATTCCCCT CAAATGCCAC AGTTCCAGAG CCTGACCCAT 
CAGTCTCCAC CCCAGCAAAA CGTCCAGCCC AAGAAGCAGG TAAAGGGCAG GGCTGAGCCA 
CAGCCCCCAG GGCCAGTCAT GGGCCGAGGC CAGGGATGCC CACCTGCCTC ACCGGCTGCC 
GTACCTATGC TGTCCCAGGA GCTGCGCCCA CCGTCAGTCG TCCAGCCCCA GCCCCTGGTG 
GTAGTGAAGG AGGAGAAGAT TCACTCACCA ATCATCCGCA GTGAGCCTTT TAGCACCTCA 
CTTCGCCCAG AGCCGCCCAA GCACCCAGAG AACATGAAGG CCCCAGTCCA CCTGCCTCAG 
CGGCCTGAGA TGAAGCCTGT AGATGTAGGG AGGCCTGTGA TCCGGCCTCC AGAGCAGAGT 
GCACCCCCAC CAGGGGCCCC TGACAAGGAC AAACAGAAAC AGGAGCCAAA AACACCAGTG 
GCGCCCAAAA AGGACCTGAA AATTAAGAAC ATGGGCTCCT GGGCCAGCCT GGTACAGAAG 
CATCCGACCA CCCCATCCTC CACAGCCAAG TCCTCAAGCG ACAGCTTTGA GCATTTCCGC 
CGCGCTGCTC GGGAGAAGGA GGAGCGGGAA AAGGCCCTGA AGGCTCAGGC TGAGCACGCA 
GAGAAGGAGA AGGAGCGGCT GAGGCAGGAG CGCATGAGGA GCCGAGAGGA TGAGGATGCG 
CTGGAACAGG CACGTCGAGC CCATGAGGAG GCACGACGGC GCCAGGAGCA GCAGCAGCAG 
CAGCAGAGAC AGGAGCAGCA GCAGCAGCAG CAACAGCAGG CAGCTGCTGT GGCCGCTGCC 
TCCGCCCCCC AGGCCCAGAG CTCCCAGCCC CAGTCTATGC TGGACCAGCA GAGGGAGTTG 
GCCCGAAAGC GAGAACAGGA GCGGAGGCGC AGGGAGGCAA TGGCAGCTAC AATTGACATG 
AATTTCCAGA GTGATCTTTT GTCAATATTT GAAGAAAATC TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763395.1
CDS	145..3912
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTSPQTQMPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEMEENKKSKAKELPPKKTKKNNSSNSNVSKKEPAPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPTGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGRGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENMKAPVHLPQRPEMKPVDVGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQRQEQQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_008765173.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765173.2

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAGAAAATCA ATGAACTGCC TACAGAAGAA 
ACCGAGATCA TGATAGTCCA GGCAAAGGGA CGAGGACGAG GGAGGAAAGA AACAGGGACA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGATGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC CCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCTCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCTCCCAT CATTGCGACC ACCCCACAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCC ACCACCATCG ACCCCATTCA TGAGCCGCCC 
TCACTGGCCC CGGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCCC CAAAGAAGGA TGTACCAGAC TCCCAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT GAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCTAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCCGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT TAAACACCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGGG AGTACAGAGA TGCCCAGGAG TTTGGTGCTG ATGTCCGATT GATGTTTTCC 
AACTGCTACA AGTACAATCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACG 
GTGTCCTCCC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGTG ATAGTTCCTC TGACAGTGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAGCT CCAGGAGCAG CTCAAAGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAACAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAATG GAGGAAAATA AAAAAAGCAA AGCCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGCACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAAGA GGACAAGTGT 
AAGCCTATGT CTTATGAGGA GAAGCGACAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCGAGGG AACCATCACT TAAAAACTCC 
AACCCTGATG AGATTGAGAT TGATTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAT 
GTGATTGCTG GTTCTTCCAA GATGAAGGGC TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCTAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCACCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCGCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCTC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC CCCCACCCCC ACCACCTCCG 
CCCTCCATGC CACAGCAGAC TGCCCCAGCG ATGAAGTCCT CGCCCCCACC CTTCATCACT 
GCCCAGGTAC CCGTCCTGGA ACCACAGCTA CCAGGCAGTG TCTTTGACCC TATTGGCCAC 
TTCACCCAGC CCATCTTGCA CCTGCCGCAG CCAGAGCTGC CTCCTCACCT GCCCCAGCCA 
CCGGAGCACA GCACTCCACC CCATCTCAAC CAGCATGCTG TGGTCTCTCC TCCAGCTTTG 
CACAATGCGC TGCCCCAACA ACCATCTCGG CCCAGCAACC GAGCTGCTGC TCTGCCCCCA 
AAGCCTGCCC GACCCCCAGC TGTGTCCCCT GCCTTGGCCC AGCCCCCCCT GCTCCCACAG 
CCACCTATGG CTCAGCCCCC CCAAGTGCTG CTGGAGGATG AAGAGCCGCC TGCCCCACCC 
CTCACCTCCA TGCAGATGCA GTTGTACCTA CAGCAGCTGC AGAAGGTGCA GCCTCCCACA 
CCACTACTCC CTTCCGTGAA GGTGCAGTCC CAACCCCCAC CACCTTTGCC GCCTCCACCC 
CACCCTTCTG TGCAGCAACA GCAGCTCCAG CAGCAGCCAC CACCACCCCC ACCTCCTCAG 
CCACAGCCAC CACCCCAGCA ACAACACCAG CCCCCACCAC GACCAGTTCA CTTGCCATCC 
ATGCCCTTCT CTGCTCACAT TCAGCAGCCC CCACCACCCA CAGGACAGCA ACCCACCCAC 
CCACCCCCAG GACAGCAGCC ACCCCCACCA CAGCCTGCCA AGCCTCAGCA AGTCATCCAG 
CATCACCCTT CACCCCGGCA CCACAAGTCA GACCCCTACT CAGCTGGTCA TCTTCGTGAA 
GCCCCTTCTC CGCTTATGAT ACATTCCCCT CAAATGCCAC AGTTCCAGAG CCTGACCCAT 
CAGTCTCCAC CCCAGCAAAA CGTCCAGCCC AAGAAGCAGG TAAAGGGCAG GGCTGAGCCA 
CAGCCCCCAG GGCCAGTCAT GGGCCGAGGC CAGGGATGCC CACCTGCCTC ACCGGCTGCC 
GTACCTATGC TGTCCCAGGA GCTGCGCCCA CCGTCAGTCG TCCAGCCCCA GCCCCTGGTG 
GTAGTGAAGG AGGAGAAGAT TCACTCACCA ATCATCCGCA GTGAGCCTTT TAGCACCTCA 
CTTCGCCCAG AGCCGCCCAA GCACCCAGAG AACATGAAGG CCCCAGTCCA CCTGCCTCAG 
CGGCCTGAGA TGAAGCCTGT AGATGTAGGG AGGCCTGTGA TCCGGCCTCC AGAGCAGAGT 
GCACCCCCAC CAGGGGCCCC TGACAAGGAC AAACAGAAAC AGGAGCCAAA AACACCAGTG 
GCGCCCAAAA AGGACCTGAA AATTAAGAAC ATGGGCTCCT GGGCCAGCCT GGTACAGAAG 
CATCCGACCA CCCCATCCTC CACAGCCAAG TCCTCAAGCG ACAGCTTTGA GCATTTCCGC 
CGCGCTGCTC GGGAGAAGGA GGAGCGGGAA AAGGCCCTGA AGGCTCAGGC TGAGCACGCA 
GAGAAGGAGA AGGAGCGGCT GAGGCAGGAG CGCATGAGGA GCCGAGAGGA TGAGGATGCG 
CTGGAACAGG CACGTCGAGC CCATGAGGAG GCACGACGGC GCCAGGAGCA GCAGCAGCAG 
CAGCAGAGAC AGGAGCAGCA GCAGCAGCAG CAACAGCAGG CAGCTGCTGT GGCCGCTGCC 
TCCGCCCCCC AGGCCCAGAG CTCCCAGCCC CAGTCTATGC TGGACCAGCA GAGGGAGTTG 
GCCCGAAAGC GAGAACAGGA GCGGAGGCGC AGGGAGGCAA TGGCAGCTAC AATTGACATG 
AATTTCCAGA GTGATCTTTT GTCAATATTT GAAGAAAATC TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_008765170.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763392.1 Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa48379
Clone ID Related Accession (Same CDS sequence)	XM_008765170.2
Accession Version	XM_008765170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bromodomain-containing protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTATC TGGTCTTTGA TGGGACTTTT TTCTTTCTGT GTAGAGTGCC TGGTGAAGAA 
TGTGATGGGA TCACTAGCAT GTCTACGGAG AGCGGCCCTG GGACAAGATT GAGAAATCTG 
CCAGTAATGG GGGATGGACT AGAAACCTCC CAAATGTCTA CAACGCAGGC CCAGGCCCAA 
CCCCAGTCAG CAAATGCAGC CAGCACCAAT CCTCCACCCC CAGAGACCTC CAACCCTAAC 
AAGCCCAAGA GGCAGACCAA CCAACTGCAA TATCTGCTCA GAGTGGTGCT CAAGACACTA 
TGGAAACACC AGTTTGCGTG GCCTTTCCAG CAGCCTGTGG ATGCCGTCAA GCTGAACCTC 
CCTGATTACT ATAAGATTAT TAAAACACCC ATGGATATGG GAACAATAAA GAAGCGCTTG 
GAAAACAACT ATTACTGGAA TGCTCAGGAA TGTATCCAGG ACTTCAACAC TATGTTTACA 
AATTGTTACA TCTATAACAA GCCTGGAGAT GACATCGTCT TAATGGCAGA AGCTCTGGAG 
AAGCTCTTCT TGCAGAAAAT CAATGAACTG CCTACAGAAG AAACCGAGAT CATGATAGTC 
CAGGCAAAGG GACGAGGACG AGGGAGGAAA GAAACAGGGA CAGCAAAGCC TGGTGTATCC 
ACGGTACCAA ACACAACTCA AGCATCAACT TCTCCGCAGA CCCAGATGCC TCAGCAGAAC 
CCTCCTCCAC CTGTGCAGGC CACAACTCAC CCCTTTCCTG CTGTCACCCC AGACCTCATT 
GCCCAGCCTC CTGTCATGAC AATGGTGCCC CCCCAGCCAC TTCAGACTCC TTCACCGGTA 
CCCCCTCAGC CACCACCCCC ACCTGCTCCA GTTCCACAGC CTGTGCAGAG TCACCCTCCC 
ATCATTGCGA CCACCCCACA GCCTGTGAAG ACAAAGAAAG GGGTGAAGAG GAAAGCAGAT 
ACCACCACCC CCACCACCAT CGACCCCATT CATGAGCCGC CCTCACTGGC CCCGGAGCCC 
AAGACCGCCA AGCTGGGTCC TCGGCGGGAG AGCAGCAGAC CTGTGAAGCC CCCAAAGAAG 
GATGTACCAG ACTCCCAGCA GCACCCAGGG CCAGAGAAGA GCAGCAAGAT CTCTGAGCAG 
CTGAAGTGCT GCAGTGGCAT CCTCAAGGAG ATGTTTGCTA AGAAACATGC TGCCTATGCC 
TGGCCTTTCT ACAAGCCCGT GGATGTGGAG GCACTGGGTC TGCACGACTA CTGTGACATC 
ATTAAACACC CCATGGACAT GAGCACAATC AAGTCTAAAC TAGAGTCCCG GGAGTACAGA 
GATGCCCAGG AGTTTGGTGC TGATGTCCGA TTGATGTTTT CCAACTGCTA CAAGTACAAT 
CCCCCTGACC ATGAAGTGGT AGCCATGGCT CGAAAACTCC AGGATGTGTT TGAAATGCGC 
TTTGCCAAGA TGCCTGATGA GCCTGAAGAG CCAGTTGTTA CGGTGTCCTC CCCTGCAGTG 
CCACCCCCTA CAAAGGTGGT AGCCCCACCC TCATCTAGTG ACAGCAGCAG TGATAGTTCC 
TCTGACAGTG ACAGTTCCAC TGACGACTCT GAGGAAGAGC GAGCCCAGCG GCTGGCTGAG 
CTCCAGGAGC AGCTCAAAGC CGTGCATGAG CAGCTTGCAG CCCTCTCACA GCCCCAACAG 
AACAAACCAA AGAAAAAGGA GAAGGACAAG AAGGAAAAGA AAAAGGAAAA GCACAAAAAG 
AAAGAAGAAA TGGAGGAAAA TAAAAAAAGC AAAGCCAAGG AACTTCCTCC CAAAAAGACA 
AAGAAAAATA ACAGCAGCAA CAGCAATGTG AGCAAGAAGG AACCAGCACC CACGAAGACC 
AAGCCGCCTC CCACATATGA ATCAGAAGAA GAGGACAAGT GTAAGCCTAT GTCTTATGAG 
GAGAAGCGAC AGCTAAGTCT AGATATCAAC AAACTTCCTG GTGAGAAGCT AGGCCGTGTA 
GTACACATAA TTCAGTCGAG GGAACCATCA CTTAAAAACT CCAACCCTGA TGAGATTGAG 
ATTGATTTTG AGACCCTGAA GCCATCTACA CTACGAGAGT TGGAGCGATA TGTCACCTCC 
TGTTTGCGGA AGAAAAGGAA ACCTCAAGCT GAAAAAGTTG ATGTGATTGC TGGTTCTTCC 
AAGATGAAGG GCTTCTCATC CTCTGAGTCG GAGAGCACCA GCGAATCTAG CTCCTCTGAC 
AGTGAAGACT CTGAAACAGA GATGGCTCCC AAGTCAAAAA AGAAGGGGCA CACTGGGAGG 
GACCAGAAAA AGCATCATCA CCATCACCAC CCACAGATGC AGCCAGCCCC AGCTCCTGTG 
CCCCAGCAGC CGCCGCCACC TCCACAGCAG CCTCCTCCAC CCCCACCTCC TCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCCCCACCC CCACCACCTC CGCCCTCCAT GCCACAGCAG 
ACTGCCCCAG CGATGAAGTC CTCGCCCCCA CCCTTCATCA CTGCCCAGGT ACCCGTCCTG 
GAACCACAGC TACCAGGCAG TGTCTTTGAC CCTATTGGCC ACTTCACCCA GCCCATCTTG 
CACCTGCCGC AGCCAGAGCT GCCTCCTCAC CTGCCCCAGC CACCGGAGCA CAGCACTCCA 
CCCCATCTCA ACCAGCATGC TGTGGTCTCT CCTCCAGCTT TGCACAATGC GCTGCCCCAA 
CAACCATCTC GGCCCAGCAA CCGAGCTGCT GCTCTGCCCC CAAAGCCTGC CCGACCCCCA 
GCTGTGTCCC CTGCCTTGGC CCAGCCCCCC CTGCTCCCAC AGCCACCTAT GGCTCAGCCC 
CCCCAAGTGC TGCTGGAGGA TGAAGAGCCG CCTGCCCCAC CCCTCACCTC CATGCAGATG 
CAGTTGTACC TACAGCAGCT GCAGAAGGTG CAGCCTCCCA CACCACTACT CCCTTCCGTG 
AAGGTGCAGT CCCAACCCCC ACCACCTTTG CCGCCTCCAC CCCACCCTTC TGTGCAGCAA 
CAGCAGCTCC AGCAGCAGCC ACCACCACCC CCACCTCCTC AGCCACAGCC ACCACCCCAG 
CAACAACACC AGCCCCCACC ACGACCAGTT CACTTGCCAT CCATGCCCTT CTCTGCTCAC 
ATTCAGCAGC CCCCACCACC CACAGGACAG CAACCCACCC ACCCACCCCC AGGACAGCAG 
CCACCCCCAC CACAGCCTGC CAAGCCTCAG CAAGTCATCC AGCATCACCC TTCACCCCGG 
CACCACAAGT CAGACCCCTA CTCAGCTGGT CATCTTCGTG AAGCCCCTTC TCCGCTTATG 
ATACATTCCC CTCAAATGCC ACAGTTCCAG AGCCTGACCC ATCAGTCTCC ACCCCAGCAA 
AACGTCCAGC CCAAGAAGCA GGTAAAGGGC AGGGCTGAGC CACAGCCCCC AGGGCCAGTC 
ATGGGCCGAG GCCAGGGATG CCCACCTGCC TCACCGGCTG CCGTACCTAT GCTGTCCCAG 
GAGCTGCGCC CACCGTCAGT CGTCCAGCCC CAGCCCCTGG TGGTAGTGAA GGAGGAGAAG 
ATTCACTCAC CAATCATCCG CAGTGAGCCT TTTAGCACCT CACTTCGCCC AGAGCCGCCC 
AAGCACCCAG AGAACATGAA GGCCCCAGTC CACCTGCCTC AGCGGCCTGA GATGAAGCCT 
GTAGATGTAG GGAGGCCTGT GATCCGGCCT CCAGAGCAGA GTGCACCCCC ACCAGGGGCC 
CCTGACAAGG ACAAACAGAA ACAGGAGCCA AAAACACCAG TGGCGCCCAA AAAGGACCTG 
AAAATTAAGA ACATGGGCTC CTGGGCCAGC CTGGTACAGA AGCATCCGAC CACCCCATCC 
TCCACAGCCA AGTCCTCAAG CGACAGCTTT GAGCATTTCC GCCGCGCTGC TCGGGAGAAG 
GAGGAGCGGG AAAAGGCCCT GAAGGCTCAG GCTGAGCACG CAGAGAAGGA GAAGGAGCGG 
CTGAGGCAGG AGCGCATGAG GAGCCGAGAG GATGAGGATG CGCTGGAACA GGCACGTCGA 
GCCCATGAGG AGGCACGACG GCGCCAGGAG CAGCAGCAGC AGCAGCAGAG ACAGGAGCAG 
CAGCAGCAGC AGCAACAGCA GGCAGCTGCT GTGGCCGCTG CCTCCGCCCC CCAGGCCCAG 
AGCTCCCAGC CCCAGTCTAT GCTGGACCAG CAGAGGGAGT TGGCCCGAAA GCGAGAACAG 
GAGCGGAGGC GCAGGGAGGC AATGGCAGCT ACAATTGACA TGAATTTCCA GAGTGATCTT 
TTGTCAATAT TTGAAGAAAA TCTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763392.1
CDS	1789..6078
Translation	MCYLVFDGTFFFLCRVPGEECDGITSMSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQSANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTSPQTQMPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEMEENKKSKAKELPPKKTKKNNSSNSNVSKKEPAPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPTGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGRGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENMKAPVHLPQRPEMKPVDVGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQRQEQQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_008765170.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765170.2

ORF Insert Sequence:

ATGTGCTATC TGGTCTTTGA TGGGACTTTT TTCTTTCTGT GTAGAGTGCC TGGTGAAGAA 
TGTGATGGGA TCACTAGCAT GTCTACGGAG AGCGGCCCTG GGACAAGATT GAGAAATCTG 
CCAGTAATGG GGGATGGACT AGAAACCTCC CAAATGTCTA CAACGCAGGC CCAGGCCCAA 
CCCCAGTCAG CAAATGCAGC CAGCACCAAT CCTCCACCCC CAGAGACCTC CAACCCTAAC 
AAGCCCAAGA GGCAGACCAA CCAACTGCAA TATCTGCTCA GAGTGGTGCT CAAGACACTA 
TGGAAACACC AGTTTGCGTG GCCTTTCCAG CAGCCTGTGG ATGCCGTCAA GCTGAACCTC 
CCTGATTACT ATAAGATTAT TAAAACACCC ATGGATATGG GAACAATAAA GAAGCGCTTG 
GAAAACAACT ATTACTGGAA TGCTCAGGAA TGTATCCAGG ACTTCAACAC TATGTTTACA 
AATTGTTACA TCTATAACAA GCCTGGAGAT GACATCGTCT TAATGGCAGA AGCTCTGGAG 
AAGCTCTTCT TGCAGAAAAT CAATGAACTG CCTACAGAAG AAACCGAGAT CATGATAGTC 
CAGGCAAAGG GACGAGGACG AGGGAGGAAA GAAACAGGGA CAGCAAAGCC TGGTGTATCC 
ACGGTACCAA ACACAACTCA AGCATCAACT TCTCCGCAGA CCCAGATGCC TCAGCAGAAC 
CCTCCTCCAC CTGTGCAGGC CACAACTCAC CCCTTTCCTG CTGTCACCCC AGACCTCATT 
GCCCAGCCTC CTGTCATGAC AATGGTGCCC CCCCAGCCAC TTCAGACTCC TTCACCGGTA 
CCCCCTCAGC CACCACCCCC ACCTGCTCCA GTTCCACAGC CTGTGCAGAG TCACCCTCCC 
ATCATTGCGA CCACCCCACA GCCTGTGAAG ACAAAGAAAG GGGTGAAGAG GAAAGCAGAT 
ACCACCACCC CCACCACCAT CGACCCCATT CATGAGCCGC CCTCACTGGC CCCGGAGCCC 
AAGACCGCCA AGCTGGGTCC TCGGCGGGAG AGCAGCAGAC CTGTGAAGCC CCCAAAGAAG 
GATGTACCAG ACTCCCAGCA GCACCCAGGG CCAGAGAAGA GCAGCAAGAT CTCTGAGCAG 
CTGAAGTGCT GCAGTGGCAT CCTCAAGGAG ATGTTTGCTA AGAAACATGC TGCCTATGCC 
TGGCCTTTCT ACAAGCCCGT GGATGTGGAG GCACTGGGTC TGCACGACTA CTGTGACATC 
ATTAAACACC CCATGGACAT GAGCACAATC AAGTCTAAAC TAGAGTCCCG GGAGTACAGA 
GATGCCCAGG AGTTTGGTGC TGATGTCCGA TTGATGTTTT CCAACTGCTA CAAGTACAAT 
CCCCCTGACC ATGAAGTGGT AGCCATGGCT CGAAAACTCC AGGATGTGTT TGAAATGCGC 
TTTGCCAAGA TGCCTGATGA GCCTGAAGAG CCAGTTGTTA CGGTGTCCTC CCCTGCAGTG 
CCACCCCCTA CAAAGGTGGT AGCCCCACCC TCATCTAGTG ACAGCAGCAG TGATAGTTCC 
TCTGACAGTG ACAGTTCCAC TGACGACTCT GAGGAAGAGC GAGCCCAGCG GCTGGCTGAG 
CTCCAGGAGC AGCTCAAAGC CGTGCATGAG CAGCTTGCAG CCCTCTCACA GCCCCAACAG 
AACAAACCAA AGAAAAAGGA GAAGGACAAG AAGGAAAAGA AAAAGGAAAA GCACAAAAAG 
AAAGAAGAAA TGGAGGAAAA TAAAAAAAGC AAAGCCAAGG AACTTCCTCC CAAAAAGACA 
AAGAAAAATA ACAGCAGCAA CAGCAATGTG AGCAAGAAGG AACCAGCACC CACGAAGACC 
AAGCCGCCTC CCACATATGA ATCAGAAGAA GAGGACAAGT GTAAGCCTAT GTCTTATGAG 
GAGAAGCGAC AGCTAAGTCT AGATATCAAC AAACTTCCTG GTGAGAAGCT AGGCCGTGTA 
GTACACATAA TTCAGTCGAG GGAACCATCA CTTAAAAACT CCAACCCTGA TGAGATTGAG 
ATTGATTTTG AGACCCTGAA GCCATCTACA CTACGAGAGT TGGAGCGATA TGTCACCTCC 
TGTTTGCGGA AGAAAAGGAA ACCTCAAGCT GAAAAAGTTG ATGTGATTGC TGGTTCTTCC 
AAGATGAAGG GCTTCTCATC CTCTGAGTCG GAGAGCACCA GCGAATCTAG CTCCTCTGAC 
AGTGAAGACT CTGAAACAGA GATGGCTCCC AAGTCAAAAA AGAAGGGGCA CACTGGGAGG 
GACCAGAAAA AGCATCATCA CCATCACCAC CCACAGATGC AGCCAGCCCC AGCTCCTGTG 
CCCCAGCAGC CGCCGCCACC TCCACAGCAG CCTCCTCCAC CCCCACCTCC TCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCCCCACCC CCACCACCTC CGCCCTCCAT GCCACAGCAG 
ACTGCCCCAG CGATGAAGTC CTCGCCCCCA CCCTTCATCA CTGCCCAGGT ACCCGTCCTG 
GAACCACAGC TACCAGGCAG TGTCTTTGAC CCTATTGGCC ACTTCACCCA GCCCATCTTG 
CACCTGCCGC AGCCAGAGCT GCCTCCTCAC CTGCCCCAGC CACCGGAGCA CAGCACTCCA 
CCCCATCTCA ACCAGCATGC TGTGGTCTCT CCTCCAGCTT TGCACAATGC GCTGCCCCAA 
CAACCATCTC GGCCCAGCAA CCGAGCTGCT GCTCTGCCCC CAAAGCCTGC CCGACCCCCA 
GCTGTGTCCC CTGCCTTGGC CCAGCCCCCC CTGCTCCCAC AGCCACCTAT GGCTCAGCCC 
CCCCAAGTGC TGCTGGAGGA TGAAGAGCCG CCTGCCCCAC CCCTCACCTC CATGCAGATG 
CAGTTGTACC TACAGCAGCT GCAGAAGGTG CAGCCTCCCA CACCACTACT CCCTTCCGTG 
AAGGTGCAGT CCCAACCCCC ACCACCTTTG CCGCCTCCAC CCCACCCTTC TGTGCAGCAA 
CAGCAGCTCC AGCAGCAGCC ACCACCACCC CCACCTCCTC AGCCACAGCC ACCACCCCAG 
CAACAACACC AGCCCCCACC ACGACCAGTT CACTTGCCAT CCATGCCCTT CTCTGCTCAC 
ATTCAGCAGC CCCCACCACC CACAGGACAG CAACCCACCC ACCCACCCCC AGGACAGCAG 
CCACCCCCAC CACAGCCTGC CAAGCCTCAG CAAGTCATCC AGCATCACCC TTCACCCCGG 
CACCACAAGT CAGACCCCTA CTCAGCTGGT CATCTTCGTG AAGCCCCTTC TCCGCTTATG 
ATACATTCCC CTCAAATGCC ACAGTTCCAG AGCCTGACCC ATCAGTCTCC ACCCCAGCAA 
AACGTCCAGC CCAAGAAGCA GGTAAAGGGC AGGGCTGAGC CACAGCCCCC AGGGCCAGTC 
ATGGGCCGAG GCCAGGGATG CCCACCTGCC TCACCGGCTG CCGTACCTAT GCTGTCCCAG 
GAGCTGCGCC CACCGTCAGT CGTCCAGCCC CAGCCCCTGG TGGTAGTGAA GGAGGAGAAG 
ATTCACTCAC CAATCATCCG CAGTGAGCCT TTTAGCACCT CACTTCGCCC AGAGCCGCCC 
AAGCACCCAG AGAACATGAA GGCCCCAGTC CACCTGCCTC AGCGGCCTGA GATGAAGCCT 
GTAGATGTAG GGAGGCCTGT GATCCGGCCT CCAGAGCAGA GTGCACCCCC ACCAGGGGCC 
CCTGACAAGG ACAAACAGAA ACAGGAGCCA AAAACACCAG TGGCGCCCAA AAAGGACCTG 
AAAATTAAGA ACATGGGCTC CTGGGCCAGC CTGGTACAGA AGCATCCGAC CACCCCATCC 
TCCACAGCCA AGTCCTCAAG CGACAGCTTT GAGCATTTCC GCCGCGCTGC TCGGGAGAAG 
GAGGAGCGGG AAAAGGCCCT GAAGGCTCAG GCTGAGCACG CAGAGAAGGA GAAGGAGCGG 
CTGAGGCAGG AGCGCATGAG GAGCCGAGAG GATGAGGATG CGCTGGAACA GGCACGTCGA 
GCCCATGAGG AGGCACGACG GCGCCAGGAG CAGCAGCAGC AGCAGCAGAG ACAGGAGCAG 
CAGCAGCAGC AGCAACAGCA GGCAGCTGCT GTGGCCGCTG CCTCCGCCCC CCAGGCCCAG 
AGCTCCCAGC CCCAGTCTAT GCTGGACCAG CAGAGGGAGT TGGCCCGAAA GCGAGAACAG 
GAGCGGAGGC GCAGGGAGGC AATGGCAGCT ACAATTGACA TGAATTTCCA GAGTGATCTT 
TTGTCAATAT TTGAAGAAAA TCTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_008765171.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763393.2 Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X2, mRNA.

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CloneID	ORa62043
Clone ID Related Accession (Same CDS sequence)	XM_008765171.2
Accession Version	XM_008765171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCGCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCTC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC CCCCACCCCC ACCACCTCCG 
CCCTCCATGC CACAGCAGAC TGCCCCAGCG ATGAAGTCCT CGCCCCCACC CTTCATCACT 
GCCCAGGTAC CCGTCCTGGA ACCACAGCTA CCAGGCAGTG TCTTTGACCC TATTGGCCAC 
TTCACCCAGC CCATCTTGCA CCTGCCGCAG CCAGAGCTGC CTCCTCACCT GCCCCAGCCA 
CCGGAGCACA GCACTCCACC CCATCTCAAC CAGCATGCTG TGGTCTCTCC TCCAGCTTTG 
CACAATGCGC TGCCCCAACA ACCATCTCGG CCCAGCAACC GAGCTGCTGC TCTGCCCCCA 
AAGCCTGCCC GACCCCCAGC TGTGTCCCCT GCCTTGGCCC AGCCCCCCCT GCTCCCACAG 
CCACCTATGG CTCAGCCCCC CCAAGTGCTG CTGGAGGATG AAGAGCCGCC TGCCCCACCC 
CTCACCTCCA TGCAGATGCA GTTGTACCTA CAGCAGCTGC AGAAGGTGCA GCCTCCCACA 
CCACTACTCC CTTCCGTGAA GGTGCAGTCC CAACCCCCAC CACCTTTGCC GCCTCCACCC 
CACCCTTCTG TGCAGCAACA GCAGCTCCAG CAGCAGCCAC CACCACCCCC ACCTCCTCAG 
CCACAGCCAC CACCCCAGCA ACAACACCAG CCCCCACCAC GACCAGTTCA CTTGCCATCC 
ATGCCCTTCT CTGCTCACAT TCAGCAGCCC CCACCACCCA CAGGACAGCA ACCCACCCAC 
CCACCCCCAG GACAGCAGCC ACCCCCACCA CAGCCTGCCA AGCCTCAGCA AGTCATCCAG 
CATCACCCTT CACCCCGGCA CCACAAGTCA GACCCCTACT CAGCTGGTCA TCTTCGTGAA 
GCCCCTTCTC CGCTTATGAT ACATTCCCCT CAAATGCCAC AGTTCCAGAG CCTGACCCAT 
CAGTCTCCAC CCCAGCAAAA CGTCCAGCCC AAGAAGCAGG TAAAGGGCAG GGCTGAGCCA 
CAGCCCCCAG GGCCAGTCAT GGGCCGAGGC CAGGGATGCC CACCTGCCTC ACCGGCTGCC 
GTACCTATGC TGTCCCAGGA GCTGCGCCCA CCGTCAGTCG TCCAGCCCCA GCCCCTGGTG 
GTAGTGAAGG AGGAGAAGAT TCACTCACCA ATCATCCGCA GTGAGCCTTT TAGCACCTCA 
CTTCGCCCAG AGCCGCCCAA GCACCCAGAG AACATGAAGG CCCCAGTCCA CCTGCCTCAG 
CGGCCTGAGA TGAAGCCTGT AGATGTAGGG AGGCCTGTGA TCCGGCCTCC AGAGCAGAGT 
GCACCCCCAC CAGGGGCCCC TGACAAGGAC AAACAGAAAC AGGAGCCAAA AACACCAGTG 
GCGCCCAAAA AGGACCTGAA AATTAAGAAC ATGGGCTCCT GGGCCAGCCT GGTACAGAAG 
CATCCGACCA CCCCATCCTC CACAGCCAAG TCCTCAAGCG ACAGCTTTGA GCATTTCCGC 
CGCGCTGCTC GGGAGAAGGA GGAGCGGGAA AAGGCCCTGA AGGCTCAGGC TGAGCACGCA 
GAGAAGGAGA AGGAGCGGCT GAGGCAGGAG CGCATGAGGA GCCGAGAGGA TGAGGATGCG 
CTGGAACAGG CACGTCGAGC CCATGAGGAG GCACGACGGC GCCAGGAGCA GCAGCAGCAG 
CAGCAGAGAC AGGAGCAGCA GCAGCAGCAG CAACAGCAGG CAGCTGCTGT GGCCGCTGCC 
TCCGCCCCCC AGGCCCAGAG CTCCCAGCCC CAGTCTATGC TGGACCAGCA GAGGGAGTTG 
GCCCGAAAGC GAGAACAGGA GCGGAGGCGC AGGGAGGCAA TGGCAGCTAC AATTGACATG 
AATTTCCAGA GTGATCTTTT GTCAATATTT GAAGAAAATC TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763393.2
CDS	109..4296
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQSANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTSPQTQMPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEMEENKKSKAKELPPKKTKKNNSSNSNVSKKEPAPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPTGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGRGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENMKAPVHLPQRPEMKPVDVGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQRQEQQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_008765171.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765171.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCGCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCTC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC CCCCACCCCC ACCACCTCCG 
CCCTCCATGC CACAGCAGAC TGCCCCAGCG ATGAAGTCCT CGCCCCCACC CTTCATCACT 
GCCCAGGTAC CCGTCCTGGA ACCACAGCTA CCAGGCAGTG TCTTTGACCC TATTGGCCAC 
TTCACCCAGC CCATCTTGCA CCTGCCGCAG CCAGAGCTGC CTCCTCACCT GCCCCAGCCA 
CCGGAGCACA GCACTCCACC CCATCTCAAC CAGCATGCTG TGGTCTCTCC TCCAGCTTTG 
CACAATGCGC TGCCCCAACA ACCATCTCGG CCCAGCAACC GAGCTGCTGC TCTGCCCCCA 
AAGCCTGCCC GACCCCCAGC TGTGTCCCCT GCCTTGGCCC AGCCCCCCCT GCTCCCACAG 
CCACCTATGG CTCAGCCCCC CCAAGTGCTG CTGGAGGATG AAGAGCCGCC TGCCCCACCC 
CTCACCTCCA TGCAGATGCA GTTGTACCTA CAGCAGCTGC AGAAGGTGCA GCCTCCCACA 
CCACTACTCC CTTCCGTGAA GGTGCAGTCC CAACCCCCAC CACCTTTGCC GCCTCCACCC 
CACCCTTCTG TGCAGCAACA GCAGCTCCAG CAGCAGCCAC CACCACCCCC ACCTCCTCAG 
CCACAGCCAC CACCCCAGCA ACAACACCAG CCCCCACCAC GACCAGTTCA CTTGCCATCC 
ATGCCCTTCT CTGCTCACAT TCAGCAGCCC CCACCACCCA CAGGACAGCA ACCCACCCAC 
CCACCCCCAG GACAGCAGCC ACCCCCACCA CAGCCTGCCA AGCCTCAGCA AGTCATCCAG 
CATCACCCTT CACCCCGGCA CCACAAGTCA GACCCCTACT CAGCTGGTCA TCTTCGTGAA 
GCCCCTTCTC CGCTTATGAT ACATTCCCCT CAAATGCCAC AGTTCCAGAG CCTGACCCAT 
CAGTCTCCAC CCCAGCAAAA CGTCCAGCCC AAGAAGCAGG TAAAGGGCAG GGCTGAGCCA 
CAGCCCCCAG GGCCAGTCAT GGGCCGAGGC CAGGGATGCC CACCTGCCTC ACCGGCTGCC 
GTACCTATGC TGTCCCAGGA GCTGCGCCCA CCGTCAGTCG TCCAGCCCCA GCCCCTGGTG 
GTAGTGAAGG AGGAGAAGAT TCACTCACCA ATCATCCGCA GTGAGCCTTT TAGCACCTCA 
CTTCGCCCAG AGCCGCCCAA GCACCCAGAG AACATGAAGG CCCCAGTCCA CCTGCCTCAG 
CGGCCTGAGA TGAAGCCTGT AGATGTAGGG AGGCCTGTGA TCCGGCCTCC AGAGCAGAGT 
GCACCCCCAC CAGGGGCCCC TGACAAGGAC AAACAGAAAC AGGAGCCAAA AACACCAGTG 
GCGCCCAAAA AGGACCTGAA AATTAAGAAC ATGGGCTCCT GGGCCAGCCT GGTACAGAAG 
CATCCGACCA CCCCATCCTC CACAGCCAAG TCCTCAAGCG ACAGCTTTGA GCATTTCCGC 
CGCGCTGCTC GGGAGAAGGA GGAGCGGGAA AAGGCCCTGA AGGCTCAGGC TGAGCACGCA 
GAGAAGGAGA AGGAGCGGCT GAGGCAGGAG CGCATGAGGA GCCGAGAGGA TGAGGATGCG 
CTGGAACAGG CACGTCGAGC CCATGAGGAG GCACGACGGC GCCAGGAGCA GCAGCAGCAG 
CAGCAGAGAC AGGAGCAGCA GCAGCAGCAG CAACAGCAGG CAGCTGCTGT GGCCGCTGCC 
TCCGCCCCCC AGGCCCAGAG CTCCCAGCCC CAGTCTATGC TGGACCAGCA GAGGGAGTTG 
GCCCGAAAGC GAGAACAGGA GCGGAGGCGC AGGGAGGCAA TGGCAGCTAC AATTGACATG 
AATTTCCAGA GTGATCTTTT GTCAATATTT GAAGAAAATC TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_017594916.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450405.1 Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X3, mRNA.

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CloneID	ORa62044
Clone ID Related Accession (Same CDS sequence)	XM_017594916.1
Accession Version	XM_017594916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bromodomain-containing protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGAGCTGC GCCCACCGTC AGTCGTCCAG CCCCAGCCCC TGGTGGTAGT GAAGGAGGAG 
AAGATTCACT CACCAATCAT CCGCAGTGAG CCTTTTAGCA CCTCACTTCG CCCAGAGCCG 
CCCAAGCACC CAGAGAACAT GAAGGCCCCA GTCCACCTGC CTCAGCGGCC TGAGATGAAG 
CCTGTAGATG TAGGGAGGCC TGTGATCCGG CCTCCAGAGC AGAGTGCACC CCCACCAGGG 
GCCCCTGACA AGGACAAACA GAAACAGGAG CCAAAAACAC CAGTGGCGCC CAAAAAGGAC 
CTGAAAATTA AGAACATGGG CTCCTGGGCC AGCCTGGTAC AGAAGCATCC GACCACCCCA 
TCCTCCACAG CCAAGTCCTC AAGCGACAGC TTTGAGCATT TCCGCCGCGC TGCTCGGGAG 
AAGGAGGAGC GGGAAAAGGC CCTGAAGGCT CAGGCTGAGC ACGCAGAGAA GGAGAAGGAG 
CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA GAGGATGAGG ATGCGCTGGA ACAGGCACGT 
CGAGCCCATG AGGAGGCACG ACGGCGCCAG GAGCAGCAGC AGCAGCAGCA GAGACAGGAG 
CAGCAGCAGC AGCAGCAACA GCAGGCAGCT GCTGTGGCCG CTGCCTCCGC CCCCCAGGCC 
CAGAGCTCCC AGCCCCAGTC TATGCTGGAC CAGCAGAGGG AGTTGGCCCG AAAGCGAGAA 
CAGGAGCGGA GGCGCAGGGA GGCAATGGCA GCTACAATTG ACATGAATTT CCAGAGTGAT 
CTTTTGTCAA TATTTGAAGA AAATCTTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450405.1
CDS	109..4221
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQSANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTSPQTQMPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEMEENKKSKAKELPPKKTKKNNSSNSNVSKKEPAPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPTGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENMKAPVHLPQRPEMKPVDVGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQRQEQQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_017594916.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus bromodomain containing 4 (Brd4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594916.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGTC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGGCAGACC 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCTGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAGAAA 
ATCAATGAAC TGCCTACAGA AGAAACCGAG ATCATGATAG TCCAGGCAAA GGGACGAGGA 
CGAGGGAGGA AAGAAACAGG GACAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGATG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCCCAGCC ACTTCAGACT CCTTCACCGG TACCCCCTCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCTC CCATCATTGC GACCACCCCA 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCCACCACC 
ATCGACCCCA TTCATGAGCC GCCCTCACTG GCCCCGGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCCCCAAAGA AGGATGTACC AGACTCCCAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTGAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC TAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCC 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATTAAACA CCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGGGAGTACA GAGATGCCCA GGAGTTTGGT 
GCTGATGTCC GATTGATGTT TTCCAACTGC TACAAGTACA ATCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACGGTGTCC TCCCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGTGATAGTT CCTCTGACAG TGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AGCTCCAGGA GCAGCTCAAA 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAAC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AATGGAGGAA 
AATAAAAAAA GCAAAGCCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGCA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AAGAGGACAA GTGTAAGCCT ATGTCTTATG AGGAGAAGCG ACAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCG 
AGGGAACCAT CACTTAAAAA CTCCAACCCT GATGAGATTG AGATTGATTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGATGTGATT GCTGGTTCTT CCAAGATGAA GGGCTTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCT AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ACCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCGCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCTCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCCCCAC CCCCACCACC TCCGCCCTCC ATGCCACAGC AGACTGCCCC AGCGATGAAG 
TCCTCGCCCC CACCCTTCAT CACTGCCCAG GTACCCGTCC TGGAACCACA GCTACCAGGC 
AGTGTCTTTG ACCCTATTGG CCACTTCACC CAGCCCATCT TGCACCTGCC GCAGCCAGAG 
CTGCCTCCTC ACCTGCCCCA GCCACCGGAG CACAGCACTC CACCCCATCT CAACCAGCAT 
GCTGTGGTCT CTCCTCCAGC TTTGCACAAT GCGCTGCCCC AACAACCATC TCGGCCCAGC 
AACCGAGCTG CTGCTCTGCC CCCAAAGCCT GCCCGACCCC CAGCTGTGTC CCCTGCCTTG 
GCCCAGCCCC CCCTGCTCCC ACAGCCACCT ATGGCTCAGC CCCCCCAAGT GCTGCTGGAG 
GATGAAGAGC CGCCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGTTGTA CCTACAGCAG 
CTGCAGAAGG TGCAGCCTCC CACACCACTA CTCCCTTCCG TGAAGGTGCA GTCCCAACCC 
CCACCACCTT TGCCGCCTCC ACCCCACCCT TCTGTGCAGC AACAGCAGCT CCAGCAGCAG 
CCACCACCAC CCCCACCTCC TCAGCCACAG CCACCACCCC AGCAACAACA CCAGCCCCCA 
CCACGACCAG TTCACTTGCC ATCCATGCCC TTCTCTGCTC ACATTCAGCA GCCCCCACCA 
CCCACAGGAC AGCAACCCAC CCACCCACCC CCAGGACAGC AGCCACCCCC ACCACAGCCT 
GCCAAGCCTC AGCAAGTCAT CCAGCATCAC CCTTCACCCC GGCACCACAA GTCAGACCCC 
TACTCAGCTG GTCATCTTCG TGAAGCCCCT TCTCCGCTTA TGATACATTC CCCTCAAATG 
CCACAGTTCC AGAGCCTGAC CCATCAGTCT CCACCCCAGC AAAACGTCCA GCCCAAGAAG 
CAGGAGCTGC GCCCACCGTC AGTCGTCCAG CCCCAGCCCC TGGTGGTAGT GAAGGAGGAG 
AAGATTCACT CACCAATCAT CCGCAGTGAG CCTTTTAGCA CCTCACTTCG CCCAGAGCCG 
CCCAAGCACC CAGAGAACAT GAAGGCCCCA GTCCACCTGC CTCAGCGGCC TGAGATGAAG 
CCTGTAGATG TAGGGAGGCC TGTGATCCGG CCTCCAGAGC AGAGTGCACC CCCACCAGGG 
GCCCCTGACA AGGACAAACA GAAACAGGAG CCAAAAACAC CAGTGGCGCC CAAAAAGGAC 
CTGAAAATTA AGAACATGGG CTCCTGGGCC AGCCTGGTAC AGAAGCATCC GACCACCCCA 
TCCTCCACAG CCAAGTCCTC AAGCGACAGC TTTGAGCATT TCCGCCGCGC TGCTCGGGAG 
AAGGAGGAGC GGGAAAAGGC CCTGAAGGCT CAGGCTGAGC ACGCAGAGAA GGAGAAGGAG 
CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA GAGGATGAGG ATGCGCTGGA ACAGGCACGT 
CGAGCCCATG AGGAGGCACG ACGGCGCCAG GAGCAGCAGC AGCAGCAGCA GAGACAGGAG 
CAGCAGCAGC AGCAGCAACA GCAGGCAGCT GCTGTGGCCG CTGCCTCCGC CCCCCAGGCC 
CAGAGCTCCC AGCCCCAGTC TATGCTGGAC CAGCAGAGGG AGTTGGCCCG AAAGCGAGAA 
CAGGAGCGGA GGCGCAGGGA GGCAATGGCA GCTACAATTG ACATGAATTT CCAGAGTGAT 
CTTTTGTCAA TATTTGAAGA AAATCTTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of BRD4 Attenuates Cardiomyocyte Apoptosis via NF-?B pathway in a Rat Model of Myocardial Infarction.
Cardiovascular therapeutics(2018 Jan)
Sun Y,Xie Y,Du L,Sun J,Liu Z

Structural Mechanism of the Oxygenase JMJD6 Recognition by the Extraterminal (ET) Domain of BRD4.
Scientific reports7(1)16272(2017 Nov)
Konuma T,Yu D,Zhao C,Ju Y,Sharma R,Ren C,Zhang Q,Zhou MM,Zeng L

Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-?-mediated Nox4 expression.
Redox biology11390-402(2017 04)
Zhou B,Mu J,Gong Y,Lu C,Zhao Y,He T,Qin Z

Inhibition of BRD4 suppresses tumor growth and enhances iodine uptake in thyroid cancer.
Biochemical and biophysical research communications469(3)679-85(2016 Jan)
Gao X,Wu X,Zhang X,Hua W,Zhang Y,Maimaiti Y,Gao Z,Zhang Y

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Brd4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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