Tbc1d20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tbc1d20
Entrez Gene ID	362237
Full Name	TBC1 domain family, member 20
General protein information	Preferred Names TBC1 domain family, member 20 Names TBC1 domain family member 20
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004281.1	NP_001004281.1	TBC1 domain family member 20
XM_008762348.1	XP_008760570.1	TBC1 domain family member 20 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TBC1D20	XP_861927.2
Mus musculus (house mouse)	Tbc1d20	NP_077158.1
Rattus norvegicus (Norway rat)	Tbc1d20	NP_001004281.1
Caenorhabditis elegans (roundworm)	tbc-20	NP_497068.2
Xenopus tropicalis (tropical clawed frog)	tbc1d20.1	NP_001016881.1
Homo sapiens (human)	TBC1D20	NP_653229.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008312	XP_317154.4
Schizosaccharomyces pombe (fission yeast)	gyp10	NP_595501.1
Bos taurus (cattle)	TBC1D20	NP_001033118.1
Gallus gallus (chicken)	TBC1D20	XP_001235015.2
Danio rerio (zebrafish)	tbc1d20	NP_001020343.1
Neurospora crassa	NCU05272	XP_961909.1
Pan troglodytes (chimpanzee)	TBC1D20	XP_003316844.1
	TBC1D20	XP_001107845.1
Drosophila melanogaster (fruit fly)	CG17883	NP_001036448.1
Magnaporthe oryzae (rice blast fungus)	MGG_06021	XP_003711868.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans.
Liegel RP, Handley MT, Ronchetti A, Brown S, Langemeyer L, Linford A, Chang B, Morris-Rosendahl DJ, Carpanini S, Posmyk R, Harthill V, Sheridan E, Abdel-Salam GM, Terhal PA, Faravelli F, Accorsi P, Giordano L, Pinelli L, Hartmann B, Ebert AD, Barr FA, Aligianis IA, Sidjanin DJ
American journal of human genetics93(6)1001-14(2013 Dec)

Role for TBC1D20 and Rab1 in hepatitis C virus replication via interaction with lipid droplet-bound nonstructural protein 5A.
Nevo-Yassaf I, Yaffe Y, Asher M, Ravid O, Eizenberg S, Henis YI, Nahmias Y, Hirschberg K, Sklan EH
Journal of virology86(12)6491-502(2012 Jun)

Analysis of Rab GTPase-activating proteins indicates that Rab1a/b and Rab43 are important for herpes simplex virus 1 secondary envelopment.
Zenner HL, Yoshimura S, Barr FA, Crump CM
Journal of virology85(16)8012-21(2011 Aug)

A Rab-GAP TBC domain protein binds hepatitis C virus NS5A and mediates viral replication.
Sklan EH, Staschke K, Oakes TM, Elazar M, Winters M, Aroeti B, Danieli T, Glenn JS
Journal of virology81(20)11096-105(2007 Oct)

Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells.
Haas AK, Yoshimura S, Stephens DJ, Preisinger C, Fuchs E, Barr FA
Journal of cell science120(Pt 17)2997-3010(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tbc1d20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tbc1d20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10344	NM_001004281.1 Latest version!	Rattus norvegicus TBC1 domain family, member 20 (Tbc1d20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa48165	XM_008762348.1 Latest version!	Rattus norvegicus TBC1 domain family, member 20 (Tbc1d20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tbc1d20 ( NM_001004281.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001004281.1 Rattus norvegicus TBC1 domain family, member 20 (Tbc1d20), mRNA.

Price & Availability↑

CloneID	ORa10344
Clone ID Related Accession (Same CDS sequence)	NM_001004281.1
Accession Version	NM_001004281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	TBC1 domain family member 20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079383.1. On Sep 10, 2004 this sequence version replaced XM_342544.1. ##Evidence-Data-START## Transcript exon combination :: BC079383.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCTCC GGCCCTCAGA GGGCGACGGC TCCGCCGGCC GCTGGAACCG CGGCTCAGGG 
AAGGCAGACT TTAATGCCAA AAGGAAAAAG AAGGTGGCGG AGATACACCA AGCTCTGAAC 
AGTGATCCCA CCGATGTGGC TGCCCTCCGA CGAATGGCTA TCAGCGAGGG CGGGCTCCTG 
ACTGATGAGA TCAGGTGCCA AGTATGGCCT AAGCTCCTCA ACGTCAACAC CGATGAGCCA 
CCGCCTATGT CAAGGAAGGA TCTTCGAGAC ATGAGCAAGG ACTACCAGCA AGTGCTGCTG 
GACGTCAGGC GGTCTCTTCG GCGCTTCCCT CCGGGCATGC CAGATGAGCA GAGAGAAGGG 
CTCCAGGAAG AGCTAATCGA TATCATCCTC CTCGTCTTGG ATCGAAACCC TCAGCTCCAC 
TACTACCAGG GCTACCATGA CATTGTGGTC ACTTTTCTGC TGGTGGTGGG CGAGAGGCTG 
GCAACATCCC TGGTAGAAAA ATTGTCCACC CACCACCTCA GGGATTTCAT GGATCCCACA 
ATGGACAACA CCAAGCACAT CTTAAATTAC CTGATGCCCA TCATTGACCA AGTGAACCCA 
GAACTTCATG ACTTCATGCA GAGTGCTGAG GTGGGGACCA TCTTCGCCCT CAGCTGGCTT 
ATCACCTGGT TTGGGCACGT CCTGTTGGAC TTCAGGCATG TGGTGAGGTT ATACGACTTC 
TTCCTGGCCT GCCACCCACT CATGCCTATT TACTTTGCAG CTGTGATCGT GCTGTACCGG 
GAGCAGGAAG TGCTGGACTG TGACTGTGAC ATGGCCTCTG TTCACCACCT GTTGTCCCAG 
ATCCCTCAGG ACCTGCCCTA TGAGACGCTC ATCAGCAGAG CAGGAGACCT CTTTGTTCAG 
TTTCCCCCTT CTGAACTGGC AAGGGAGGCG GCTGCTCAGC AGGAGGCGGA GAGAACGGCA 
GCCTCTACTT TCAAAGACTT TGAGCTGGCG TCAACTCAGC AGAGGCCAGA CATGGTGCTG 
CGGCAGCGGT TTCGGGGACT TCTGAGGCCT GAGGCTCGAA CAAAAGATGT CCTGACCAAA 
CCAAGGACCA ACCGCTTTGT AAAACTGGCA GTGATGGGGC TGACGGTGGC CCTAGGAGCA 
GCAGCACTGG CAGTGGTGAA GAGTGCCCTG GAGTGGGCCC CTAAGTTCCA GCTGCAGCTG 
TTCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004281.1
CDS	58..1266
Translation	MALRPSEGDGSAGRWNRGSGKADFNAKRKKKVAEIHQALNSDPTDVAALRRMAISEGGLLTDEIRCQVWPKLLNVNTDEPPPMSRKDLRDMSKDYQQVLLDVRRSLRRFPPGMPDEQREGLQEELIDIILLVLDRNPQLHYYQGYHDIVVTFLLVVGERLATSLVEKLSTHHLRDFMDPTMDNTKHILNYLMPIIDQVNPELHDFMQSAEVGTIFALSWLITWFGHVLLDFRHVVRLYDFFLACHPLMPIYFAAVIVLYREQEVLDCDCDMASVHHLLSQIPQDLPYETLISRAGDLFVQFPPSELAREAAAQQEAERTAASTFKDFELASTQQRPDMVLRQRFRGLLRPEARTKDVLTKPRTNRFVKLAVMGLTVALGAAALAVVKSALEWAPKFQLQLFP

Target ORF information:

RefSeq Version	NM_001004281.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TBC1 domain family, member 20 (Tbc1d20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004281.1

ORF Insert Sequence:

ATGGCCCTCC GGCCCTCAGA GGGCGACGGC TCCGCCGGCC GCTGGAACCG CGGCTCAGGG 
AAGGCAGACT TTAATGCCAA AAGGAAAAAG AAGGTGGCGG AGATACACCA AGCTCTGAAC 
AGTGATCCCA CCGATGTGGC TGCCCTCCGA CGAATGGCTA TCAGCGAGGG CGGGCTCCTG 
ACTGATGAGA TCAGGTGCCA AGTATGGCCT AAGCTCCTCA ACGTCAACAC CGATGAGCCA 
CCGCCTATGT CAAGGAAGGA TCTTCGAGAC ATGAGCAAGG ACTACCAGCA AGTGCTGCTG 
GACGTCAGGC GGTCTCTTCG GCGCTTCCCT CCGGGCATGC CAGATGAGCA GAGAGAAGGG 
CTCCAGGAAG AGCTAATCGA TATCATCCTC CTCGTCTTGG ATCGAAACCC TCAGCTCCAC 
TACTACCAGG GCTACCATGA CATTGTGGTC ACTTTTCTGC TGGTGGTGGG CGAGAGGCTG 
GCAACATCCC TGGTAGAAAA ATTGTCCACC CACCACCTCA GGGATTTCAT GGATCCCACA 
ATGGACAACA CCAAGCACAT CTTAAATTAC CTGATGCCCA TCATTGACCA AGTGAACCCA 
GAACTTCATG ACTTCATGCA GAGTGCTGAG GTGGGGACCA TCTTCGCCCT CAGCTGGCTT 
ATCACCTGGT TTGGGCACGT CCTGTTGGAC TTCAGGCATG TGGTGAGGTT ATACGACTTC 
TTCCTGGCCT GCCACCCACT CATGCCTATT TACTTTGCAG CTGTGATCGT GCTGTACCGG 
GAGCAGGAAG TGCTGGACTG TGACTGTGAC ATGGCCTCTG TTCACCACCT GTTGTCCCAG 
ATCCCTCAGG ACCTGCCCTA TGAGACGCTC ATCAGCAGAG CAGGAGACCT CTTTGTTCAG 
TTTCCCCCTT CTGAACTGGC AAGGGAGGCG GCTGCTCAGC AGGAGGCGGA GAGAACGGCA 
GCCTCTACTT TCAAAGACTT TGAGCTGGCG TCAACTCAGC AGAGGCCAGA CATGGTGCTG 
CGGCAGCGGT TTCGGGGACT TCTGAGGCCT GAGGCTCGAA CAAAAGATGT CCTGACCAAA 
CCAAGGACCA ACCGCTTTGT AAAACTGGCA GTGATGGGGC TGACGGTGGC CCTAGGAGCA 
GCAGCACTGG CAGTGGTGAA GAGTGCCCTG GAGTGGGCCC CTAAGTTCCA GCTGCAGCTG 
TTCCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbc1d20 ( XM_008762348.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760570.1 Rattus norvegicus TBC1 domain family, member 20 (Tbc1d20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa48165
Clone ID Related Accession (Same CDS sequence)	XM_008762348.1
Accession Version	XM_008762348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	TBC1 domain family member 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCC GGCCCTCAGA GGGCGACGGC TCCGCCGGCC GCTGGAACCG CGGCTCAGGG 
AAGGCAGACT TTAATGCCAA AAGGAAAAAG AAGGTGGCGG AGATACACCA AGCTCTGAAC 
AGTGATCCCA CCGATGTGGC TGCCCTCCGA CGAATGGCTA TCAGCGAGGG CGGGCTCCTG 
ACTGATGAGA TCAGGTGCCA AGTATGGCCT AAGCTCCTCA ACGTCAACAC CGATGAGCCA 
CCGCCTATGT CAAGGAAGGA TCTTCGAGAC ATGAGCAAGG ACTACCAGCA AGTGCTGCTG 
GACGTCAGGC GGTCTCTTCG GCGCTTCCCT CCGGGCATGC CAGATGAGCA GAGAGAAGGG 
CTCCAGGAAG AGCTAATCGA TATCATCCTC CTCGTCTTGG ATCGAAACCC TCAGCTCCAC 
TACTACCAGG GCTACCATGA CATTGTGGTC ACTTTTCTGC TGGTGGTGGG CGAGAGGCTG 
GCAACATCCC TGGTAGAAAA ATTGTCCACC CACCACCTCA GGGATTTCAT GGATCCCACA 
ATGGACAACA CCAAGCACAT CTTAAATTAC CTGATGCCCA TCATTGACCA AGTGAACCCA 
GAACTTCATG ACTTCATGCA GAGTGCTGAG GTGGGGACCA TCTTCGCCCT CAGCTGGCTT 
ATCACCTGGT TTGGGCACGT CCTGTTGGAC TTCAGGCATG TGATCGTGCT GTACCGGGAG 
CAGGAAGTGC TGGACTGTGA CTGTGACATG GCCTCTGTTC ACCACCTGTT GTCCCAGATC 
CCTCAGGACC TGCCCTATGA GACGCTCATC AGCAGAGCAG GAGACCTCTT TGTTCAGTTT 
CCCCCTTCTG AACTGGCAAG GGAGGCGGCT GCTCAGCAGG AGGCGGAGAG AACGGCAGCC 
TCTACTTTCA AAGACTTTGA GCTGGCGTCA ACTCAGCAGA GGCCAGACAT GGTGCTGCGG 
CAGCGGTTTC GGGGACTTCT GAGGCCTGAG GCTCGAACAA AAGATGTCCT GACCAAACCA 
AGGACCAACC GCTTTGTAAA ACTGGCAGTG ATGGGGCTGA CGGTGGCCCT AGGAGCAGCA 
GCACTGGCAG TGGTGAAGAG TGCCCTGGAG TGGGCCCCTA AGTTCCAGCT GCAGCTGTTC 
CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760570.1
CDS	207..1352
Translation	MALRPSEGDGSAGRWNRGSGKADFNAKRKKKVAEIHQALNSDPTDVAALRRMAISEGGLLTDEIRCQVWPKLLNVNTDEPPPMSRKDLRDMSKDYQQVLLDVRRSLRRFPPGMPDEQREGLQEELIDIILLVLDRNPQLHYYQGYHDIVVTFLLVVGERLATSLVEKLSTHHLRDFMDPTMDNTKHILNYLMPIIDQVNPELHDFMQSAEVGTIFALSWLITWFGHVLLDFRHVIVLYREQEVLDCDCDMASVHHLLSQIPQDLPYETLISRAGDLFVQFPPSELAREAAAQQEAERTAASTFKDFELASTQQRPDMVLRQRFRGLLRPEARTKDVLTKPRTNRFVKLAVMGLTVALGAAALAVVKSALEWAPKFQLQLFP

Target ORF information:

RefSeq Version	XM_008762348.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TBC1 domain family, member 20 (Tbc1d20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762348.1

ORF Insert Sequence:

ATGGCCCTCC GGCCCTCAGA GGGCGACGGC TCCGCCGGCC GCTGGAACCG CGGCTCAGGG 
AAGGCAGACT TTAATGCCAA AAGGAAAAAG AAGGTGGCGG AGATACACCA AGCTCTGAAC 
AGTGATCCCA CCGATGTGGC TGCCCTCCGA CGAATGGCTA TCAGCGAGGG CGGGCTCCTG 
ACTGATGAGA TCAGGTGCCA AGTATGGCCT AAGCTCCTCA ACGTCAACAC CGATGAGCCA 
CCGCCTATGT CAAGGAAGGA TCTTCGAGAC ATGAGCAAGG ACTACCAGCA AGTGCTGCTG 
GACGTCAGGC GGTCTCTTCG GCGCTTCCCT CCGGGCATGC CAGATGAGCA GAGAGAAGGG 
CTCCAGGAAG AGCTAATCGA TATCATCCTC CTCGTCTTGG ATCGAAACCC TCAGCTCCAC 
TACTACCAGG GCTACCATGA CATTGTGGTC ACTTTTCTGC TGGTGGTGGG CGAGAGGCTG 
GCAACATCCC TGGTAGAAAA ATTGTCCACC CACCACCTCA GGGATTTCAT GGATCCCACA 
ATGGACAACA CCAAGCACAT CTTAAATTAC CTGATGCCCA TCATTGACCA AGTGAACCCA 
GAACTTCATG ACTTCATGCA GAGTGCTGAG GTGGGGACCA TCTTCGCCCT CAGCTGGCTT 
ATCACCTGGT TTGGGCACGT CCTGTTGGAC TTCAGGCATG TGATCGTGCT GTACCGGGAG 
CAGGAAGTGC TGGACTGTGA CTGTGACATG GCCTCTGTTC ACCACCTGTT GTCCCAGATC 
CCTCAGGACC TGCCCTATGA GACGCTCATC AGCAGAGCAG GAGACCTCTT TGTTCAGTTT 
CCCCCTTCTG AACTGGCAAG GGAGGCGGCT GCTCAGCAGG AGGCGGAGAG AACGGCAGCC 
TCTACTTTCA AAGACTTTGA GCTGGCGTCA ACTCAGCAGA GGCCAGACAT GGTGCTGCGG 
CAGCGGTTTC GGGGACTTCT GAGGCCTGAG GCTCGAACAA AAGATGTCCT GACCAAACCA 
AGGACCAACC GCTTTGTAAA ACTGGCAGTG ATGGGGCTGA CGGTGGCCCT AGGAGCAGCA 
GCACTGGCAG TGGTGAAGAG TGCCCTGGAG TGGGCCCCTA AGTTCCAGCT GCAGCTGTTC 
CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans.
American journal of human genetics93(6)1001-14(2013 Dec)
Liegel RP,Handley MT,Ronchetti A,Brown S,Langemeyer L,Linford A,Chang B,Morris-Rosendahl DJ,Carpanini S,Posmyk R,Harthill V,Sheridan E,Abdel-Salam GM,Terhal PA,Faravelli F,Accorsi P,Giordano L,Pinelli L,Hartmann B,Ebert AD,Barr FA,Aligianis IA,Sidjanin DJ

Role for TBC1D20 and Rab1 in hepatitis C virus replication via interaction with lipid droplet-bound nonstructural protein 5A.
Journal of virology86(12)6491-502(2012 Jun)
Nevo-Yassaf I,Yaffe Y,Asher M,Ravid O,Eizenberg S,Henis YI,Nahmias Y,Hirschberg K,Sklan EH

Analysis of Rab GTPase-activating proteins indicates that Rab1a/b and Rab43 are important for herpes simplex virus 1 secondary envelopment.
Journal of virology85(16)8012-21(2011 Aug)
Zenner HL,Yoshimura S,Barr FA,Crump CM

A Rab-GAP TBC domain protein binds hepatitis C virus NS5A and mediates viral replication.
Journal of virology81(20)11096-105(2007 Oct)
Sklan EH,Staschke K,Oakes TM,Elazar M,Winters M,Aroeti B,Danieli T,Glenn JS

Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells.
Journal of cell science120(Pt 17)2997-3010(2007 Sep)
Haas AK,Yoshimura S,Stephens DJ,Preisinger C,Fuchs E,Barr FA