Ube3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube3a
Entrez Gene ID	361585
Full Name	ubiquitin protein ligase E3A
General protein information	Preferred Names ubiquitin protein ligase E3A Names ubiquitin-protein ligase E3A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589369.1	XP_017444858.1	ubiquitin-protein ligase E3A isoform X1
XM_008759401.2	XP_008757623.1	ubiquitin-protein ligase E3A isoform X3
XM_006229313.3	XP_006229375.1	ubiquitin-protein ligase E3A isoform X3
XM_008759400.2	XP_008757622.1	ubiquitin-protein ligase E3A isoform X3
NM_001191837.2	NP_001178766.1	ubiquitin-protein ligase E3A
XM_006229310.3	XP_006229372.1	ubiquitin-protein ligase E3A isoform X1
XM_008759399.2	XP_008757621.1	ubiquitin-protein ligase E3A isoform X2
XM_017589368.1	XP_017444857.1	ubiquitin-protein ligase E3A isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ube3a	NP_035798.2
Rattus norvegicus (Norway rat)	Ube3a	NP_001178766.1
Danio rerio (zebrafish)	ube3a	NP_001007319.1
Pan troglodytes (chimpanzee)	UBE3A	XP_001161311.1
Bos taurus (cattle)	UBE3A	NP_001091932.1
Gallus gallus (chicken)	UBE3A	XP_004938531.1
Drosophila melanogaster (fruit fly)	Ube3a	NP_648452.1
Homo sapiens (human)	UBE3A	NP_570854.1
	UBE3A	XP_001108661.1
Canis lupus familiaris (dog)	UBE3A	XP_005618288.1
Xenopus tropicalis (tropical clawed frog)	ube3a	NP_001001213.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012366	XP_551819.3

Gene Ontology
Bibliography

Related articles in PubMed

Ube3a reinstatement identifies distinct developmental windows in a murine Angelman syndrome model.
Silva-Santos S, van Woerden GM, Bruinsma CF, Mientjes E, Jolfaei MA, Distel B, Kushner SA, Elgersma Y
The Journal of clinical investigation125(5)2069-76(2015 May)

A coding-independent function of an alternative Ube3a transcript during neuronal development.
Valluy J, Bicker S, Aksoy-Aksel A, Lackinger M, Sumer S, Fiore R, W?st T, Seffer D, Metge F, Dieterich C, W?hr M, Schwarting R, Schratt G
Nature neuroscience18(5)666-73(2015 May)

Towards a therapy for Angelman syndrome by targeting a long non-coding RNA.
Meng L, Ward AJ, Chun S, Bennett CF, Beaudet AL, Rigo F
Nature518(7539)409-12(2015 Feb)

Long-term abstinence from developmental cocaine exposure alters Arc/Arg3.1 modulation in the rat medial prefrontal cortex.
Caffino L, Giannotti G, Malpighi C, Racagni G, Filip M, Fumagalli F
Neurotoxicity research26(3)299-306(2014 Oct)

The E3 ubiquitin ligase UBE3A is an integral component of the molecular circadian clock through regulating the BMAL1 transcription factor.
Gossan NC, Zhang F, Guo B, Jin D, Yoshitane H, Yao A, Glossop N, Zhang YQ, Fukada Y, Meng QJ
Nucleic acids research42(9)5765-75(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ube3a1 RNA function was independent of its coding sequence but instead required a unique 3' untranslated region and an intact microRNA pathway. Ube3a1 RNA acts as a dendritic competing endogenous RNA.
Title: A coding-independent function of an alternative Ube3a transcript during neuronal development.

The following Ube3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07779	NM_001191837.2 Latest version!	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa07779	XM_006229310.3 Latest version!	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa07779	XM_017589369.1 Latest version!	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa07779	XM_017589368.1 Latest version!	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa22858	XM_008759399.2 Latest version!	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa22859	XM_008759401.2 Latest version!	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa22859	XM_006229313.3 Latest version!	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa22859	XM_008759400.2 Latest version!	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube3a ( NM_001191837.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178766.1 Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), mRNA.

Price & Availability↑

CloneID	ORa07779
Clone ID Related Accession (Same CDS sequence)	XM_017589369.1 , NM_001191837.2 , XM_006229310.3 , XM_017589368.1
Accession Version	NM_001191837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-protein ligase E3A
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AABR07003599.1 and AABR07003600.1. On Jul 7, 2012 this sequence version replaced NM_001191837.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAATCCCAGT CTGAGGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAGCGCT ACTACCATCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTCTGTGCTT CCTGTCCAAC TTTTCTTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCT CTTGAGCTTT ATAAAATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAGAA CTCAAAAGGT 
GCATCTAACA ACTCAGAGAT AAAAATGAAC AAGAAGGAAG AGTTTAAAGA GGTGGACTAC 
CTAACTGAAG AGAAAGTATA TGAAATTTAT GAATTTTGTA GGGAGAGAGA GGATTACTCC 
CCTTTAATTC GTGTAATTGG AAGAATATTT TCTAGTGCTG AGGCACTGGT TCAGAGCTTT 
CGGAAAGTCA AACAGCACAC TAAGGAGGAA TTGAAATCTC TTCAAGAGAA GGATGAAGAC 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCG TGTTCTGCTG CTGCTATGGA AGAAGACTCA 
GAAGCATCTT CTTCAAGGAT GGGTGATAGC TCACAGGGAG ACAACAATGT ACAAAAATTA 
GGTCCTGATG ATGTGACTGT GGATATTGAT GCTATAAGAA GGGTCTACAG CAGTTTGCTC 
TCTGATGAAA AAATAGAAAC TGCCTTCCTG AATGCACTTG TATATCTGTC ACCTAACGTG 
GAATGTGATT TGACATATCA TAATGTGTAC ACTCGAGACC CTAATTATCT CAATTTGTTC 
ATTATTGTAA TGGAAAATAG GAATCTCCAC AGTCCTGAAT ATCTGGAAAT GGCATTGCCG 
TTATTTTGCA AAGCTATGAG TAAGCTACCC CTTGCAGCTC AAGGAAAACT GATTAGGCTG 
TGGTCTAAAT ACAGTGCTGA CCAGATTCGG AGAATGATGG AAACATTTCA GCAACTTATT 
ACCTACAAAG TCATAAGCAA TGAATTTAAT AGCCGAAATC TAGTGAATGA TGATGATGCC 
ATTGTTGCTG CTTCAAAGTG TTTGAAAATG GTTTACTATG CAAATGTAGT GGGAGGGGAC 
GTGGACACAA ATCATAATGA GGAAGATGAT GAAGAGCCTA TACCTGAGTC CAGTGAATTG 
ACACTTCAGG AGCTTCTGGG AGAGGAAAGA AGAAATAAGA AAGGTCCTCG AGTGGATCCA 
CTAGAAACCG AAATTGGCGT TAAAACTCTA GATTGTCGAA AACCACTTAT CTCCTTTGAA 
GAATTCATTA ATGAACCACT GAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTTTTC 
AAAGTTGAAA CAGAGAACAA ATTCTCTTTT ATGACATGTC CCTTTATATT GAATGCTGTC 
ACAAAGAATC TGGGATTGTA TTATGACAAT AGAATTCGCA TGTACAGTGA AAGAAGAATC 
ACTGTTCTTT ACAGCCTAGT TCAAGGACAG CAGTTGAATC CATATTTGAG ACTTAAAGTC 
AGACGTGACC ATATTATAGA TGATGCACTT GTCCGGCTAG AGATGATCGC TATGGAAAAT 
CCTGCAGACT TGAAGAAACA GTTGTATGTG GAATTTGAAG GAGAACAAGG AGTAGATGAG 
GGAGGCGTTT CCAAAGAGTT TTTTCAGTTG GTTGTGGAGG AAATCTTTAA TCCAGATATT 
GGTATGTTCA CATACGATGA AGCTACAAGA TTATTTTGGT TTAATCCATC TTCTTTTGAA 
ACTGAGGGTC AATTTACTCT GATTGGCATA GTCCTGGGTC TGGCTATTTA CAATAACTGT 
ATACTGGATG TCCATTTTCC CATGGTTGTG TACAGGAAGC TAATGGGGAA AAAAGGAACG 
TTTTGTGACT TGGGAGACTC TCACCCAATC TTATATCAGA GTTTAAAGGA TTTATTGGAA 
TATGAAGGGA ATGTGGAAGA TGATATGATG ATCACTTTCC AGATATCACA GACAGATCTT 
TTTGGTAACC CAATGATGTA TGATCTAAAA GAAAATGGTG ATAAAATTCC AATTACAAAT 
GAAAACAGGA AGGAATTTGT CAGTCTCTAT TCAGACTACA TTCTCAATAA ATCTGTAGAA 
AAACAATTCA AGGCATTTCG CAGAGGTTTT CATATGGTGA CTAATGAATC ACCCTTAAAA 
TACTTATTCA GACCAGAAGA AATTGAATTA CTTATATGTG GAAGCCGGAA TCTCGATTTC 
CAGGCACTAG AAGAAACTAC AGAGTATGAT GGTGGCTATA CGAGAGAATC TGTTGTGATT 
AGGGAGTTCT GGGAAATTGT TCATTCGTTT ACAGATGAAC AGAAAAGACT TTTCCTGCAG 
TTTACAACAG GCACAGACAG AGCACCTGTT GGAGGACTCG GAAAATTGAA GATGATTATA 
GCCAAAAATG GCCCAGACAC AGAAAGGTTA CCTACATCTC ATACTTGCTT TAATGTCCTT 
TTACTTCCGG AATATTCAAG CAAAGAAAAA CTTAAAGAGA GATTGCTGAA GGCCATCACA 
TATGCCAAAG GATTTGGCAT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178766.1
CDS	671..3277
Translation	MATACKRSSGESQSEDIEASRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEEFKEVDYLTEEKVYEIYEFCREREDYSPLIRVIGRIFSSAEALVQSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDIDAIRRVYSSLLSDEKIETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETEIGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDEATRLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFCDLGDSHPILYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVSLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	NM_001191837.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191837.2

ORF Insert Sequence:

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAATCCCAGT CTGAGGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAGCGCT ACTACCATCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTCTGTGCTT CCTGTCCAAC TTTTCTTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCT CTTGAGCTTT ATAAAATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAGAA CTCAAAAGGT 
GCATCTAACA ACTCAGAGAT AAAAATGAAC AAGAAGGAAG AGTTTAAAGA GGTGGACTAC 
CTAACTGAAG AGAAAGTATA TGAAATTTAT GAATTTTGTA GGGAGAGAGA GGATTACTCC 
CCTTTAATTC GTGTAATTGG AAGAATATTT TCTAGTGCTG AGGCACTGGT TCAGAGCTTT 
CGGAAAGTCA AACAGCACAC TAAGGAGGAA TTGAAATCTC TTCAAGAGAA GGATGAAGAC 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCG TGTTCTGCTG CTGCTATGGA AGAAGACTCA 
GAAGCATCTT CTTCAAGGAT GGGTGATAGC TCACAGGGAG ACAACAATGT ACAAAAATTA 
GGTCCTGATG ATGTGACTGT GGATATTGAT GCTATAAGAA GGGTCTACAG CAGTTTGCTC 
TCTGATGAAA AAATAGAAAC TGCCTTCCTG AATGCACTTG TATATCTGTC ACCTAACGTG 
GAATGTGATT TGACATATCA TAATGTGTAC ACTCGAGACC CTAATTATCT CAATTTGTTC 
ATTATTGTAA TGGAAAATAG GAATCTCCAC AGTCCTGAAT ATCTGGAAAT GGCATTGCCG 
TTATTTTGCA AAGCTATGAG TAAGCTACCC CTTGCAGCTC AAGGAAAACT GATTAGGCTG 
TGGTCTAAAT ACAGTGCTGA CCAGATTCGG AGAATGATGG AAACATTTCA GCAACTTATT 
ACCTACAAAG TCATAAGCAA TGAATTTAAT AGCCGAAATC TAGTGAATGA TGATGATGCC 
ATTGTTGCTG CTTCAAAGTG TTTGAAAATG GTTTACTATG CAAATGTAGT GGGAGGGGAC 
GTGGACACAA ATCATAATGA GGAAGATGAT GAAGAGCCTA TACCTGAGTC CAGTGAATTG 
ACACTTCAGG AGCTTCTGGG AGAGGAAAGA AGAAATAAGA AAGGTCCTCG AGTGGATCCA 
CTAGAAACCG AAATTGGCGT TAAAACTCTA GATTGTCGAA AACCACTTAT CTCCTTTGAA 
GAATTCATTA ATGAACCACT GAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTTTTC 
AAAGTTGAAA CAGAGAACAA ATTCTCTTTT ATGACATGTC CCTTTATATT GAATGCTGTC 
ACAAAGAATC TGGGATTGTA TTATGACAAT AGAATTCGCA TGTACAGTGA AAGAAGAATC 
ACTGTTCTTT ACAGCCTAGT TCAAGGACAG CAGTTGAATC CATATTTGAG ACTTAAAGTC 
AGACGTGACC ATATTATAGA TGATGCACTT GTCCGGCTAG AGATGATCGC TATGGAAAAT 
CCTGCAGACT TGAAGAAACA GTTGTATGTG GAATTTGAAG GAGAACAAGG AGTAGATGAG 
GGAGGCGTTT CCAAAGAGTT TTTTCAGTTG GTTGTGGAGG AAATCTTTAA TCCAGATATT 
GGTATGTTCA CATACGATGA AGCTACAAGA TTATTTTGGT TTAATCCATC TTCTTTTGAA 
ACTGAGGGTC AATTTACTCT GATTGGCATA GTCCTGGGTC TGGCTATTTA CAATAACTGT 
ATACTGGATG TCCATTTTCC CATGGTTGTG TACAGGAAGC TAATGGGGAA AAAAGGAACG 
TTTTGTGACT TGGGAGACTC TCACCCAATC TTATATCAGA GTTTAAAGGA TTTATTGGAA 
TATGAAGGGA ATGTGGAAGA TGATATGATG ATCACTTTCC AGATATCACA GACAGATCTT 
TTTGGTAACC CAATGATGTA TGATCTAAAA GAAAATGGTG ATAAAATTCC AATTACAAAT 
GAAAACAGGA AGGAATTTGT CAGTCTCTAT TCAGACTACA TTCTCAATAA ATCTGTAGAA 
AAACAATTCA AGGCATTTCG CAGAGGTTTT CATATGGTGA CTAATGAATC ACCCTTAAAA 
TACTTATTCA GACCAGAAGA AATTGAATTA CTTATATGTG GAAGCCGGAA TCTCGATTTC 
CAGGCACTAG AAGAAACTAC AGAGTATGAT GGTGGCTATA CGAGAGAATC TGTTGTGATT 
AGGGAGTTCT GGGAAATTGT TCATTCGTTT ACAGATGAAC AGAAAAGACT TTTCCTGCAG 
TTTACAACAG GCACAGACAG AGCACCTGTT GGAGGACTCG GAAAATTGAA GATGATTATA 
GCCAAAAATG GCCCAGACAC AGAAAGGTTA CCTACATCTC ATACTTGCTT TAATGTCCTT 
TTACTTCCGG AATATTCAAG CAAAGAAAAA CTTAAAGAGA GATTGCTGAA GGCCATCACA 
TATGCCAAAG GATTTGGCAT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube3a ( XM_017589369.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444858.1 Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa07779
Clone ID Related Accession (Same CDS sequence)	XM_017589369.1 , NM_001191837.2 , XM_006229310.3 , XM_017589368.1
Accession Version	XM_017589369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-protein ligase E3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAATCCCAGT CTGAGGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAGCGCT ACTACCATCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTCTGTGCTT CCTGTCCAAC TTTTCTTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCT CTTGAGCTTT ATAAAATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAGAA CTCAAAAGGT 
GCATCTAACA ACTCAGAGAT AAAAATGAAC AAGAAGGAAG AGTTTAAAGA GGTGGACTAC 
CTAACTGAAG AGAAAGTATA TGAAATTTAT GAATTTTGTA GGGAGAGAGA GGATTACTCC 
CCTTTAATTC GTGTAATTGG AAGAATATTT TCTAGTGCTG AGGCACTGGT TCAGAGCTTT 
CGGAAAGTCA AACAGCACAC TAAGGAGGAA TTGAAATCTC TTCAAGAGAA GGATGAAGAC 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCG TGTTCTGCTG CTGCTATGGA AGAAGACTCA 
GAAGCATCTT CTTCAAGGAT GGGTGATAGC TCACAGGGAG ACAACAATGT ACAAAAATTA 
GGTCCTGATG ATGTGACTGT GGATATTGAT GCTATAAGAA GGGTCTACAG CAGTTTGCTC 
TCTGATGAAA AAATAGAAAC TGCCTTCCTG AATGCACTTG TATATCTGTC ACCTAACGTG 
GAATGTGATT TGACATATCA TAATGTGTAC ACTCGAGACC CTAATTATCT CAATTTGTTC 
ATTATTGTAA TGGAAAATAG GAATCTCCAC AGTCCTGAAT ATCTGGAAAT GGCATTGCCG 
TTATTTTGCA AAGCTATGAG TAAGCTACCC CTTGCAGCTC AAGGAAAACT GATTAGGCTG 
TGGTCTAAAT ACAGTGCTGA CCAGATTCGG AGAATGATGG AAACATTTCA GCAACTTATT 
ACCTACAAAG TCATAAGCAA TGAATTTAAT AGCCGAAATC TAGTGAATGA TGATGATGCC 
ATTGTTGCTG CTTCAAAGTG TTTGAAAATG GTTTACTATG CAAATGTAGT GGGAGGGGAC 
GTGGACACAA ATCATAATGA GGAAGATGAT GAAGAGCCTA TACCTGAGTC CAGTGAATTG 
ACACTTCAGG AGCTTCTGGG AGAGGAAAGA AGAAATAAGA AAGGTCCTCG AGTGGATCCA 
CTAGAAACCG AAATTGGCGT TAAAACTCTA GATTGTCGAA AACCACTTAT CTCCTTTGAA 
GAATTCATTA ATGAACCACT GAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTTTTC 
AAAGTTGAAA CAGAGAACAA ATTCTCTTTT ATGACATGTC CCTTTATATT GAATGCTGTC 
ACAAAGAATC TGGGATTGTA TTATGACAAT AGAATTCGCA TGTACAGTGA AAGAAGAATC 
ACTGTTCTTT ACAGCCTAGT TCAAGGACAG CAGTTGAATC CATATTTGAG ACTTAAAGTC 
AGACGTGACC ATATTATAGA TGATGCACTT GTCCGGCTAG AGATGATCGC TATGGAAAAT 
CCTGCAGACT TGAAGAAACA GTTGTATGTG GAATTTGAAG GAGAACAAGG AGTAGATGAG 
GGAGGCGTTT CCAAAGAGTT TTTTCAGTTG GTTGTGGAGG AAATCTTTAA TCCAGATATT 
GGTATGTTCA CATACGATGA AGCTACAAGA TTATTTTGGT TTAATCCATC TTCTTTTGAA 
ACTGAGGGTC AATTTACTCT GATTGGCATA GTCCTGGGTC TGGCTATTTA CAATAACTGT 
ATACTGGATG TCCATTTTCC CATGGTTGTG TACAGGAAGC TAATGGGGAA AAAAGGAACG 
TTTTGTGACT TGGGAGACTC TCACCCAATC TTATATCAGA GTTTAAAGGA TTTATTGGAA 
TATGAAGGGA ATGTGGAAGA TGATATGATG ATCACTTTCC AGATATCACA GACAGATCTT 
TTTGGTAACC CAATGATGTA TGATCTAAAA GAAAATGGTG ATAAAATTCC AATTACAAAT 
GAAAACAGGA AGGAATTTGT CAGTCTCTAT TCAGACTACA TTCTCAATAA ATCTGTAGAA 
AAACAATTCA AGGCATTTCG CAGAGGTTTT CATATGGTGA CTAATGAATC ACCCTTAAAA 
TACTTATTCA GACCAGAAGA AATTGAATTA CTTATATGTG GAAGCCGGAA TCTCGATTTC 
CAGGCACTAG AAGAAACTAC AGAGTATGAT GGTGGCTATA CGAGAGAATC TGTTGTGATT 
AGGGAGTTCT GGGAAATTGT TCATTCGTTT ACAGATGAAC AGAAAAGACT TTTCCTGCAG 
TTTACAACAG GCACAGACAG AGCACCTGTT GGAGGACTCG GAAAATTGAA GATGATTATA 
GCCAAAAATG GCCCAGACAC AGAAAGGTTA CCTACATCTC ATACTTGCTT TAATGTCCTT 
TTACTTCCGG AATATTCAAG CAAAGAAAAA CTTAAAGAGA GATTGCTGAA GGCCATCACA 
TATGCCAAAG GATTTGGCAT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444858.1
CDS	351..2957
Translation	MATACKRSSGESQSEDIEASRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEEFKEVDYLTEEKVYEIYEFCREREDYSPLIRVIGRIFSSAEALVQSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDIDAIRRVYSSLLSDEKIETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETEIGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDEATRLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFCDLGDSHPILYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVSLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_017589369.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589369.1

ORF Insert Sequence:

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAATCCCAGT CTGAGGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAGCGCT ACTACCATCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTCTGTGCTT CCTGTCCAAC TTTTCTTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCT CTTGAGCTTT ATAAAATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAGAA CTCAAAAGGT 
GCATCTAACA ACTCAGAGAT AAAAATGAAC AAGAAGGAAG AGTTTAAAGA GGTGGACTAC 
CTAACTGAAG AGAAAGTATA TGAAATTTAT GAATTTTGTA GGGAGAGAGA GGATTACTCC 
CCTTTAATTC GTGTAATTGG AAGAATATTT TCTAGTGCTG AGGCACTGGT TCAGAGCTTT 
CGGAAAGTCA AACAGCACAC TAAGGAGGAA TTGAAATCTC TTCAAGAGAA GGATGAAGAC 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCG TGTTCTGCTG CTGCTATGGA AGAAGACTCA 
GAAGCATCTT CTTCAAGGAT GGGTGATAGC TCACAGGGAG ACAACAATGT ACAAAAATTA 
GGTCCTGATG ATGTGACTGT GGATATTGAT GCTATAAGAA GGGTCTACAG CAGTTTGCTC 
TCTGATGAAA AAATAGAAAC TGCCTTCCTG AATGCACTTG TATATCTGTC ACCTAACGTG 
GAATGTGATT TGACATATCA TAATGTGTAC ACTCGAGACC CTAATTATCT CAATTTGTTC 
ATTATTGTAA TGGAAAATAG GAATCTCCAC AGTCCTGAAT ATCTGGAAAT GGCATTGCCG 
TTATTTTGCA AAGCTATGAG TAAGCTACCC CTTGCAGCTC AAGGAAAACT GATTAGGCTG 
TGGTCTAAAT ACAGTGCTGA CCAGATTCGG AGAATGATGG AAACATTTCA GCAACTTATT 
ACCTACAAAG TCATAAGCAA TGAATTTAAT AGCCGAAATC TAGTGAATGA TGATGATGCC 
ATTGTTGCTG CTTCAAAGTG TTTGAAAATG GTTTACTATG CAAATGTAGT GGGAGGGGAC 
GTGGACACAA ATCATAATGA GGAAGATGAT GAAGAGCCTA TACCTGAGTC CAGTGAATTG 
ACACTTCAGG AGCTTCTGGG AGAGGAAAGA AGAAATAAGA AAGGTCCTCG AGTGGATCCA 
CTAGAAACCG AAATTGGCGT TAAAACTCTA GATTGTCGAA AACCACTTAT CTCCTTTGAA 
GAATTCATTA ATGAACCACT GAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTTTTC 
AAAGTTGAAA CAGAGAACAA ATTCTCTTTT ATGACATGTC CCTTTATATT GAATGCTGTC 
ACAAAGAATC TGGGATTGTA TTATGACAAT AGAATTCGCA TGTACAGTGA AAGAAGAATC 
ACTGTTCTTT ACAGCCTAGT TCAAGGACAG CAGTTGAATC CATATTTGAG ACTTAAAGTC 
AGACGTGACC ATATTATAGA TGATGCACTT GTCCGGCTAG AGATGATCGC TATGGAAAAT 
CCTGCAGACT TGAAGAAACA GTTGTATGTG GAATTTGAAG GAGAACAAGG AGTAGATGAG 
GGAGGCGTTT CCAAAGAGTT TTTTCAGTTG GTTGTGGAGG AAATCTTTAA TCCAGATATT 
GGTATGTTCA CATACGATGA AGCTACAAGA TTATTTTGGT TTAATCCATC TTCTTTTGAA 
ACTGAGGGTC AATTTACTCT GATTGGCATA GTCCTGGGTC TGGCTATTTA CAATAACTGT 
ATACTGGATG TCCATTTTCC CATGGTTGTG TACAGGAAGC TAATGGGGAA AAAAGGAACG 
TTTTGTGACT TGGGAGACTC TCACCCAATC TTATATCAGA GTTTAAAGGA TTTATTGGAA 
TATGAAGGGA ATGTGGAAGA TGATATGATG ATCACTTTCC AGATATCACA GACAGATCTT 
TTTGGTAACC CAATGATGTA TGATCTAAAA GAAAATGGTG ATAAAATTCC AATTACAAAT 
GAAAACAGGA AGGAATTTGT CAGTCTCTAT TCAGACTACA TTCTCAATAA ATCTGTAGAA 
AAACAATTCA AGGCATTTCG CAGAGGTTTT CATATGGTGA CTAATGAATC ACCCTTAAAA 
TACTTATTCA GACCAGAAGA AATTGAATTA CTTATATGTG GAAGCCGGAA TCTCGATTTC 
CAGGCACTAG AAGAAACTAC AGAGTATGAT GGTGGCTATA CGAGAGAATC TGTTGTGATT 
AGGGAGTTCT GGGAAATTGT TCATTCGTTT ACAGATGAAC AGAAAAGACT TTTCCTGCAG 
TTTACAACAG GCACAGACAG AGCACCTGTT GGAGGACTCG GAAAATTGAA GATGATTATA 
GCCAAAAATG GCCCAGACAC AGAAAGGTTA CCTACATCTC ATACTTGCTT TAATGTCCTT 
TTACTTCCGG AATATTCAAG CAAAGAAAAA CTTAAAGAGA GATTGCTGAA GGCCATCACA 
TATGCCAAAG GATTTGGCAT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube3a ( XM_006229310.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229372.1 Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa07779
Clone ID Related Accession (Same CDS sequence)	XM_017589369.1 , NM_001191837.2 , XM_006229310.3 , XM_017589368.1
Accession Version	XM_006229310.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-protein ligase E3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229310.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAATCCCAGT CTGAGGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAGCGCT ACTACCATCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTCTGTGCTT CCTGTCCAAC TTTTCTTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCT CTTGAGCTTT ATAAAATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAGAA CTCAAAAGGT 
GCATCTAACA ACTCAGAGAT AAAAATGAAC AAGAAGGAAG AGTTTAAAGA GGTGGACTAC 
CTAACTGAAG AGAAAGTATA TGAAATTTAT GAATTTTGTA GGGAGAGAGA GGATTACTCC 
CCTTTAATTC GTGTAATTGG AAGAATATTT TCTAGTGCTG AGGCACTGGT TCAGAGCTTT 
CGGAAAGTCA AACAGCACAC TAAGGAGGAA TTGAAATCTC TTCAAGAGAA GGATGAAGAC 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCG TGTTCTGCTG CTGCTATGGA AGAAGACTCA 
GAAGCATCTT CTTCAAGGAT GGGTGATAGC TCACAGGGAG ACAACAATGT ACAAAAATTA 
GGTCCTGATG ATGTGACTGT GGATATTGAT GCTATAAGAA GGGTCTACAG CAGTTTGCTC 
TCTGATGAAA AAATAGAAAC TGCCTTCCTG AATGCACTTG TATATCTGTC ACCTAACGTG 
GAATGTGATT TGACATATCA TAATGTGTAC ACTCGAGACC CTAATTATCT CAATTTGTTC 
ATTATTGTAA TGGAAAATAG GAATCTCCAC AGTCCTGAAT ATCTGGAAAT GGCATTGCCG 
TTATTTTGCA AAGCTATGAG TAAGCTACCC CTTGCAGCTC AAGGAAAACT GATTAGGCTG 
TGGTCTAAAT ACAGTGCTGA CCAGATTCGG AGAATGATGG AAACATTTCA GCAACTTATT 
ACCTACAAAG TCATAAGCAA TGAATTTAAT AGCCGAAATC TAGTGAATGA TGATGATGCC 
ATTGTTGCTG CTTCAAAGTG TTTGAAAATG GTTTACTATG CAAATGTAGT GGGAGGGGAC 
GTGGACACAA ATCATAATGA GGAAGATGAT GAAGAGCCTA TACCTGAGTC CAGTGAATTG 
ACACTTCAGG AGCTTCTGGG AGAGGAAAGA AGAAATAAGA AAGGTCCTCG AGTGGATCCA 
CTAGAAACCG AAATTGGCGT TAAAACTCTA GATTGTCGAA AACCACTTAT CTCCTTTGAA 
GAATTCATTA ATGAACCACT GAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTTTTC 
AAAGTTGAAA CAGAGAACAA ATTCTCTTTT ATGACATGTC CCTTTATATT GAATGCTGTC 
ACAAAGAATC TGGGATTGTA TTATGACAAT AGAATTCGCA TGTACAGTGA AAGAAGAATC 
ACTGTTCTTT ACAGCCTAGT TCAAGGACAG CAGTTGAATC CATATTTGAG ACTTAAAGTC 
AGACGTGACC ATATTATAGA TGATGCACTT GTCCGGCTAG AGATGATCGC TATGGAAAAT 
CCTGCAGACT TGAAGAAACA GTTGTATGTG GAATTTGAAG GAGAACAAGG AGTAGATGAG 
GGAGGCGTTT CCAAAGAGTT TTTTCAGTTG GTTGTGGAGG AAATCTTTAA TCCAGATATT 
GGTATGTTCA CATACGATGA AGCTACAAGA TTATTTTGGT TTAATCCATC TTCTTTTGAA 
ACTGAGGGTC AATTTACTCT GATTGGCATA GTCCTGGGTC TGGCTATTTA CAATAACTGT 
ATACTGGATG TCCATTTTCC CATGGTTGTG TACAGGAAGC TAATGGGGAA AAAAGGAACG 
TTTTGTGACT TGGGAGACTC TCACCCAATC TTATATCAGA GTTTAAAGGA TTTATTGGAA 
TATGAAGGGA ATGTGGAAGA TGATATGATG ATCACTTTCC AGATATCACA GACAGATCTT 
TTTGGTAACC CAATGATGTA TGATCTAAAA GAAAATGGTG ATAAAATTCC AATTACAAAT 
GAAAACAGGA AGGAATTTGT CAGTCTCTAT TCAGACTACA TTCTCAATAA ATCTGTAGAA 
AAACAATTCA AGGCATTTCG CAGAGGTTTT CATATGGTGA CTAATGAATC ACCCTTAAAA 
TACTTATTCA GACCAGAAGA AATTGAATTA CTTATATGTG GAAGCCGGAA TCTCGATTTC 
CAGGCACTAG AAGAAACTAC AGAGTATGAT GGTGGCTATA CGAGAGAATC TGTTGTGATT 
AGGGAGTTCT GGGAAATTGT TCATTCGTTT ACAGATGAAC AGAAAAGACT TTTCCTGCAG 
TTTACAACAG GCACAGACAG AGCACCTGTT GGAGGACTCG GAAAATTGAA GATGATTATA 
GCCAAAAATG GCCCAGACAC AGAAAGGTTA CCTACATCTC ATACTTGCTT TAATGTCCTT 
TTACTTCCGG AATATTCAAG CAAAGAAAAA CTTAAAGAGA GATTGCTGAA GGCCATCACA 
TATGCCAAAG GATTTGGCAT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229372.1
CDS	1521..4127
Translation	MATACKRSSGESQSEDIEASRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEEFKEVDYLTEEKVYEIYEFCREREDYSPLIRVIGRIFSSAEALVQSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDIDAIRRVYSSLLSDEKIETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETEIGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDEATRLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFCDLGDSHPILYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVSLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_006229310.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229310.3

ORF Insert Sequence:

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAATCCCAGT CTGAGGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAGCGCT ACTACCATCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTCTGTGCTT CCTGTCCAAC TTTTCTTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCT CTTGAGCTTT ATAAAATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAGAA CTCAAAAGGT 
GCATCTAACA ACTCAGAGAT AAAAATGAAC AAGAAGGAAG AGTTTAAAGA GGTGGACTAC 
CTAACTGAAG AGAAAGTATA TGAAATTTAT GAATTTTGTA GGGAGAGAGA GGATTACTCC 
CCTTTAATTC GTGTAATTGG AAGAATATTT TCTAGTGCTG AGGCACTGGT TCAGAGCTTT 
CGGAAAGTCA AACAGCACAC TAAGGAGGAA TTGAAATCTC TTCAAGAGAA GGATGAAGAC 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCG TGTTCTGCTG CTGCTATGGA AGAAGACTCA 
GAAGCATCTT CTTCAAGGAT GGGTGATAGC TCACAGGGAG ACAACAATGT ACAAAAATTA 
GGTCCTGATG ATGTGACTGT GGATATTGAT GCTATAAGAA GGGTCTACAG CAGTTTGCTC 
TCTGATGAAA AAATAGAAAC TGCCTTCCTG AATGCACTTG TATATCTGTC ACCTAACGTG 
GAATGTGATT TGACATATCA TAATGTGTAC ACTCGAGACC CTAATTATCT CAATTTGTTC 
ATTATTGTAA TGGAAAATAG GAATCTCCAC AGTCCTGAAT ATCTGGAAAT GGCATTGCCG 
TTATTTTGCA AAGCTATGAG TAAGCTACCC CTTGCAGCTC AAGGAAAACT GATTAGGCTG 
TGGTCTAAAT ACAGTGCTGA CCAGATTCGG AGAATGATGG AAACATTTCA GCAACTTATT 
ACCTACAAAG TCATAAGCAA TGAATTTAAT AGCCGAAATC TAGTGAATGA TGATGATGCC 
ATTGTTGCTG CTTCAAAGTG TTTGAAAATG GTTTACTATG CAAATGTAGT GGGAGGGGAC 
GTGGACACAA ATCATAATGA GGAAGATGAT GAAGAGCCTA TACCTGAGTC CAGTGAATTG 
ACACTTCAGG AGCTTCTGGG AGAGGAAAGA AGAAATAAGA AAGGTCCTCG AGTGGATCCA 
CTAGAAACCG AAATTGGCGT TAAAACTCTA GATTGTCGAA AACCACTTAT CTCCTTTGAA 
GAATTCATTA ATGAACCACT GAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTTTTC 
AAAGTTGAAA CAGAGAACAA ATTCTCTTTT ATGACATGTC CCTTTATATT GAATGCTGTC 
ACAAAGAATC TGGGATTGTA TTATGACAAT AGAATTCGCA TGTACAGTGA AAGAAGAATC 
ACTGTTCTTT ACAGCCTAGT TCAAGGACAG CAGTTGAATC CATATTTGAG ACTTAAAGTC 
AGACGTGACC ATATTATAGA TGATGCACTT GTCCGGCTAG AGATGATCGC TATGGAAAAT 
CCTGCAGACT TGAAGAAACA GTTGTATGTG GAATTTGAAG GAGAACAAGG AGTAGATGAG 
GGAGGCGTTT CCAAAGAGTT TTTTCAGTTG GTTGTGGAGG AAATCTTTAA TCCAGATATT 
GGTATGTTCA CATACGATGA AGCTACAAGA TTATTTTGGT TTAATCCATC TTCTTTTGAA 
ACTGAGGGTC AATTTACTCT GATTGGCATA GTCCTGGGTC TGGCTATTTA CAATAACTGT 
ATACTGGATG TCCATTTTCC CATGGTTGTG TACAGGAAGC TAATGGGGAA AAAAGGAACG 
TTTTGTGACT TGGGAGACTC TCACCCAATC TTATATCAGA GTTTAAAGGA TTTATTGGAA 
TATGAAGGGA ATGTGGAAGA TGATATGATG ATCACTTTCC AGATATCACA GACAGATCTT 
TTTGGTAACC CAATGATGTA TGATCTAAAA GAAAATGGTG ATAAAATTCC AATTACAAAT 
GAAAACAGGA AGGAATTTGT CAGTCTCTAT TCAGACTACA TTCTCAATAA ATCTGTAGAA 
AAACAATTCA AGGCATTTCG CAGAGGTTTT CATATGGTGA CTAATGAATC ACCCTTAAAA 
TACTTATTCA GACCAGAAGA AATTGAATTA CTTATATGTG GAAGCCGGAA TCTCGATTTC 
CAGGCACTAG AAGAAACTAC AGAGTATGAT GGTGGCTATA CGAGAGAATC TGTTGTGATT 
AGGGAGTTCT GGGAAATTGT TCATTCGTTT ACAGATGAAC AGAAAAGACT TTTCCTGCAG 
TTTACAACAG GCACAGACAG AGCACCTGTT GGAGGACTCG GAAAATTGAA GATGATTATA 
GCCAAAAATG GCCCAGACAC AGAAAGGTTA CCTACATCTC ATACTTGCTT TAATGTCCTT 
TTACTTCCGG AATATTCAAG CAAAGAAAAA CTTAAAGAGA GATTGCTGAA GGCCATCACA 
TATGCCAAAG GATTTGGCAT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube3a ( XM_017589368.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444857.1 Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07779
Clone ID Related Accession (Same CDS sequence)	XM_017589369.1 , NM_001191837.2 , XM_006229310.3 , XM_017589368.1
Accession Version	XM_017589368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-protein ligase E3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAATCCCAGT CTGAGGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAGCGCT ACTACCATCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTCTGTGCTT CCTGTCCAAC TTTTCTTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCT CTTGAGCTTT ATAAAATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAGAA CTCAAAAGGT 
GCATCTAACA ACTCAGAGAT AAAAATGAAC AAGAAGGAAG AGTTTAAAGA GGTGGACTAC 
CTAACTGAAG AGAAAGTATA TGAAATTTAT GAATTTTGTA GGGAGAGAGA GGATTACTCC 
CCTTTAATTC GTGTAATTGG AAGAATATTT TCTAGTGCTG AGGCACTGGT TCAGAGCTTT 
CGGAAAGTCA AACAGCACAC TAAGGAGGAA TTGAAATCTC TTCAAGAGAA GGATGAAGAC 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCG TGTTCTGCTG CTGCTATGGA AGAAGACTCA 
GAAGCATCTT CTTCAAGGAT GGGTGATAGC TCACAGGGAG ACAACAATGT ACAAAAATTA 
GGTCCTGATG ATGTGACTGT GGATATTGAT GCTATAAGAA GGGTCTACAG CAGTTTGCTC 
TCTGATGAAA AAATAGAAAC TGCCTTCCTG AATGCACTTG TATATCTGTC ACCTAACGTG 
GAATGTGATT TGACATATCA TAATGTGTAC ACTCGAGACC CTAATTATCT CAATTTGTTC 
ATTATTGTAA TGGAAAATAG GAATCTCCAC AGTCCTGAAT ATCTGGAAAT GGCATTGCCG 
TTATTTTGCA AAGCTATGAG TAAGCTACCC CTTGCAGCTC AAGGAAAACT GATTAGGCTG 
TGGTCTAAAT ACAGTGCTGA CCAGATTCGG AGAATGATGG AAACATTTCA GCAACTTATT 
ACCTACAAAG TCATAAGCAA TGAATTTAAT AGCCGAAATC TAGTGAATGA TGATGATGCC 
ATTGTTGCTG CTTCAAAGTG TTTGAAAATG GTTTACTATG CAAATGTAGT GGGAGGGGAC 
GTGGACACAA ATCATAATGA GGAAGATGAT GAAGAGCCTA TACCTGAGTC CAGTGAATTG 
ACACTTCAGG AGCTTCTGGG AGAGGAAAGA AGAAATAAGA AAGGTCCTCG AGTGGATCCA 
CTAGAAACCG AAATTGGCGT TAAAACTCTA GATTGTCGAA AACCACTTAT CTCCTTTGAA 
GAATTCATTA ATGAACCACT GAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTTTTC 
AAAGTTGAAA CAGAGAACAA ATTCTCTTTT ATGACATGTC CCTTTATATT GAATGCTGTC 
ACAAAGAATC TGGGATTGTA TTATGACAAT AGAATTCGCA TGTACAGTGA AAGAAGAATC 
ACTGTTCTTT ACAGCCTAGT TCAAGGACAG CAGTTGAATC CATATTTGAG ACTTAAAGTC 
AGACGTGACC ATATTATAGA TGATGCACTT GTCCGGCTAG AGATGATCGC TATGGAAAAT 
CCTGCAGACT TGAAGAAACA GTTGTATGTG GAATTTGAAG GAGAACAAGG AGTAGATGAG 
GGAGGCGTTT CCAAAGAGTT TTTTCAGTTG GTTGTGGAGG AAATCTTTAA TCCAGATATT 
GGTATGTTCA CATACGATGA AGCTACAAGA TTATTTTGGT TTAATCCATC TTCTTTTGAA 
ACTGAGGGTC AATTTACTCT GATTGGCATA GTCCTGGGTC TGGCTATTTA CAATAACTGT 
ATACTGGATG TCCATTTTCC CATGGTTGTG TACAGGAAGC TAATGGGGAA AAAAGGAACG 
TTTTGTGACT TGGGAGACTC TCACCCAATC TTATATCAGA GTTTAAAGGA TTTATTGGAA 
TATGAAGGGA ATGTGGAAGA TGATATGATG ATCACTTTCC AGATATCACA GACAGATCTT 
TTTGGTAACC CAATGATGTA TGATCTAAAA GAAAATGGTG ATAAAATTCC AATTACAAAT 
GAAAACAGGA AGGAATTTGT CAGTCTCTAT TCAGACTACA TTCTCAATAA ATCTGTAGAA 
AAACAATTCA AGGCATTTCG CAGAGGTTTT CATATGGTGA CTAATGAATC ACCCTTAAAA 
TACTTATTCA GACCAGAAGA AATTGAATTA CTTATATGTG GAAGCCGGAA TCTCGATTTC 
CAGGCACTAG AAGAAACTAC AGAGTATGAT GGTGGCTATA CGAGAGAATC TGTTGTGATT 
AGGGAGTTCT GGGAAATTGT TCATTCGTTT ACAGATGAAC AGAAAAGACT TTTCCTGCAG 
TTTACAACAG GCACAGACAG AGCACCTGTT GGAGGACTCG GAAAATTGAA GATGATTATA 
GCCAAAAATG GCCCAGACAC AGAAAGGTTA CCTACATCTC ATACTTGCTT TAATGTCCTT 
TTACTTCCGG AATATTCAAG CAAAGAAAAA CTTAAAGAGA GATTGCTGAA GGCCATCACA 
TATGCCAAAG GATTTGGCAT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444857.1
CDS	687..3293
Translation	MATACKRSSGESQSEDIEASRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEEFKEVDYLTEEKVYEIYEFCREREDYSPLIRVIGRIFSSAEALVQSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDIDAIRRVYSSLLSDEKIETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETEIGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDEATRLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFCDLGDSHPILYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVSLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_017589368.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589368.1

ORF Insert Sequence:

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAATCCCAGT CTGAGGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAGCGCT ACTACCATCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTCTGTGCTT CCTGTCCAAC TTTTCTTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCT CTTGAGCTTT ATAAAATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAGAA CTCAAAAGGT 
GCATCTAACA ACTCAGAGAT AAAAATGAAC AAGAAGGAAG AGTTTAAAGA GGTGGACTAC 
CTAACTGAAG AGAAAGTATA TGAAATTTAT GAATTTTGTA GGGAGAGAGA GGATTACTCC 
CCTTTAATTC GTGTAATTGG AAGAATATTT TCTAGTGCTG AGGCACTGGT TCAGAGCTTT 
CGGAAAGTCA AACAGCACAC TAAGGAGGAA TTGAAATCTC TTCAAGAGAA GGATGAAGAC 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCG TGTTCTGCTG CTGCTATGGA AGAAGACTCA 
GAAGCATCTT CTTCAAGGAT GGGTGATAGC TCACAGGGAG ACAACAATGT ACAAAAATTA 
GGTCCTGATG ATGTGACTGT GGATATTGAT GCTATAAGAA GGGTCTACAG CAGTTTGCTC 
TCTGATGAAA AAATAGAAAC TGCCTTCCTG AATGCACTTG TATATCTGTC ACCTAACGTG 
GAATGTGATT TGACATATCA TAATGTGTAC ACTCGAGACC CTAATTATCT CAATTTGTTC 
ATTATTGTAA TGGAAAATAG GAATCTCCAC AGTCCTGAAT ATCTGGAAAT GGCATTGCCG 
TTATTTTGCA AAGCTATGAG TAAGCTACCC CTTGCAGCTC AAGGAAAACT GATTAGGCTG 
TGGTCTAAAT ACAGTGCTGA CCAGATTCGG AGAATGATGG AAACATTTCA GCAACTTATT 
ACCTACAAAG TCATAAGCAA TGAATTTAAT AGCCGAAATC TAGTGAATGA TGATGATGCC 
ATTGTTGCTG CTTCAAAGTG TTTGAAAATG GTTTACTATG CAAATGTAGT GGGAGGGGAC 
GTGGACACAA ATCATAATGA GGAAGATGAT GAAGAGCCTA TACCTGAGTC CAGTGAATTG 
ACACTTCAGG AGCTTCTGGG AGAGGAAAGA AGAAATAAGA AAGGTCCTCG AGTGGATCCA 
CTAGAAACCG AAATTGGCGT TAAAACTCTA GATTGTCGAA AACCACTTAT CTCCTTTGAA 
GAATTCATTA ATGAACCACT GAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTTTTC 
AAAGTTGAAA CAGAGAACAA ATTCTCTTTT ATGACATGTC CCTTTATATT GAATGCTGTC 
ACAAAGAATC TGGGATTGTA TTATGACAAT AGAATTCGCA TGTACAGTGA AAGAAGAATC 
ACTGTTCTTT ACAGCCTAGT TCAAGGACAG CAGTTGAATC CATATTTGAG ACTTAAAGTC 
AGACGTGACC ATATTATAGA TGATGCACTT GTCCGGCTAG AGATGATCGC TATGGAAAAT 
CCTGCAGACT TGAAGAAACA GTTGTATGTG GAATTTGAAG GAGAACAAGG AGTAGATGAG 
GGAGGCGTTT CCAAAGAGTT TTTTCAGTTG GTTGTGGAGG AAATCTTTAA TCCAGATATT 
GGTATGTTCA CATACGATGA AGCTACAAGA TTATTTTGGT TTAATCCATC TTCTTTTGAA 
ACTGAGGGTC AATTTACTCT GATTGGCATA GTCCTGGGTC TGGCTATTTA CAATAACTGT 
ATACTGGATG TCCATTTTCC CATGGTTGTG TACAGGAAGC TAATGGGGAA AAAAGGAACG 
TTTTGTGACT TGGGAGACTC TCACCCAATC TTATATCAGA GTTTAAAGGA TTTATTGGAA 
TATGAAGGGA ATGTGGAAGA TGATATGATG ATCACTTTCC AGATATCACA GACAGATCTT 
TTTGGTAACC CAATGATGTA TGATCTAAAA GAAAATGGTG ATAAAATTCC AATTACAAAT 
GAAAACAGGA AGGAATTTGT CAGTCTCTAT TCAGACTACA TTCTCAATAA ATCTGTAGAA 
AAACAATTCA AGGCATTTCG CAGAGGTTTT CATATGGTGA CTAATGAATC ACCCTTAAAA 
TACTTATTCA GACCAGAAGA AATTGAATTA CTTATATGTG GAAGCCGGAA TCTCGATTTC 
CAGGCACTAG AAGAAACTAC AGAGTATGAT GGTGGCTATA CGAGAGAATC TGTTGTGATT 
AGGGAGTTCT GGGAAATTGT TCATTCGTTT ACAGATGAAC AGAAAAGACT TTTCCTGCAG 
TTTACAACAG GCACAGACAG AGCACCTGTT GGAGGACTCG GAAAATTGAA GATGATTATA 
GCCAAAAATG GCCCAGACAC AGAAAGGTTA CCTACATCTC ATACTTGCTT TAATGTCCTT 
TTACTTCCGG AATATTCAAG CAAAGAAAAA CTTAAAGAGA GATTGCTGAA GGCCATCACA 
TATGCCAAAG GATTTGGCAT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube3a ( XM_008759399.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757621.1 Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa22858
Clone ID Related Accession (Same CDS sequence)	XM_008759399.2
Accession Version	XM_008759399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-protein ligase E3A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATTC TGAAATATAC CACCATCCTA AGGATGAGGA AGCTGAGACA CAGAAAGGCT 
AAGAAGCGAG CAGCTGCAAA GCATCTAATA GAGCGCTACT ACCATCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAATGAGTTC TGTGCTTCCT GTCCAACTTT TCTTCGTATG 
GATAACAATG CAGCAGCTAT TAAAGCTCTT GAGCTTTATA AAATTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAGAACTC AAAAGGTGCA 
TCTAACAACT CAGAGATAAA AATGAACAAG AAGGAAGAGT TTAAAGAGGT GGACTACCTA 
ACTGAAGAGA AAGTATATGA AATTTATGAA TTTTGTAGGG AGAGAGAGGA TTACTCCCCT 
TTAATTCGTG TAATTGGAAG AATATTTTCT AGTGCTGAGG CACTGGTTCA GAGCTTTCGG 
AAAGTCAAAC AGCACACTAA GGAGGAATTG AAATCTCTTC AAGAGAAGGA TGAAGACAAG 
GATGAAGATG AAAAGGAAAA AGCTGCGTGT TCTGCTGCTG CTATGGAAGA AGACTCAGAA 
GCATCTTCTT CAAGGATGGG TGATAGCTCA CAGGGAGACA ACAATGTACA AAAATTAGGT 
CCTGATGATG TGACTGTGGA TATTGATGCT ATAAGAAGGG TCTACAGCAG TTTGCTCTCT 
GATGAAAAAA TAGAAACTGC CTTCCTGAAT GCACTTGTAT ATCTGTCACC TAACGTGGAA 
TGTGATTTGA CATATCATAA TGTGTACACT CGAGACCCTA ATTATCTCAA TTTGTTCATT 
ATTGTAATGG AAAATAGGAA TCTCCACAGT CCTGAATATC TGGAAATGGC ATTGCCGTTA 
TTTTGCAAAG CTATGAGTAA GCTACCCCTT GCAGCTCAAG GAAAACTGAT TAGGCTGTGG 
TCTAAATACA GTGCTGACCA GATTCGGAGA ATGATGGAAA CATTTCAGCA ACTTATTACC 
TACAAAGTCA TAAGCAATGA ATTTAATAGC CGAAATCTAG TGAATGATGA TGATGCCATT 
GTTGCTGCTT CAAAGTGTTT GAAAATGGTT TACTATGCAA ATGTAGTGGG AGGGGACGTG 
GACACAAATC ATAATGAGGA AGATGATGAA GAGCCTATAC CTGAGTCCAG TGAATTGACA 
CTTCAGGAGC TTCTGGGAGA GGAAAGAAGA AATAAGAAAG GTCCTCGAGT GGATCCACTA 
GAAACCGAAA TTGGCGTTAA AACTCTAGAT TGTCGAAAAC CACTTATCTC CTTTGAAGAA 
TTCATTAATG AACCACTGAA TGATGTTCTA GAAATGGACA AAGATTATAC TTTTTTCAAA 
GTTGAAACAG AGAACAAATT CTCTTTTATG ACATGTCCCT TTATATTGAA TGCTGTCACA 
AAGAATCTGG GATTGTATTA TGACAATAGA ATTCGCATGT ACAGTGAAAG AAGAATCACT 
GTTCTTTACA GCCTAGTTCA AGGACAGCAG TTGAATCCAT ATTTGAGACT TAAAGTCAGA 
CGTGACCATA TTATAGATGA TGCACTTGTC CGGCTAGAGA TGATCGCTAT GGAAAATCCT 
GCAGACTTGA AGAAACAGTT GTATGTGGAA TTTGAAGGAG AACAAGGAGT AGATGAGGGA 
GGCGTTTCCA AAGAGTTTTT TCAGTTGGTT GTGGAGGAAA TCTTTAATCC AGATATTGGT 
ATGTTCACAT ACGATGAAGC TACAAGATTA TTTTGGTTTA ATCCATCTTC TTTTGAAACT 
GAGGGTCAAT TTACTCTGAT TGGCATAGTC CTGGGTCTGG CTATTTACAA TAACTGTATA 
CTGGATGTCC ATTTTCCCAT GGTTGTGTAC AGGAAGCTAA TGGGGAAAAA AGGAACGTTT 
TGTGACTTGG GAGACTCTCA CCCAATCTTA TATCAGAGTT TAAAGGATTT ATTGGAATAT 
GAAGGGAATG TGGAAGATGA TATGATGATC ACTTTCCAGA TATCACAGAC AGATCTTTTT 
GGTAACCCAA TGATGTATGA TCTAAAAGAA AATGGTGATA AAATTCCAAT TACAAATGAA 
AACAGGAAGG AATTTGTCAG TCTCTATTCA GACTACATTC TCAATAAATC TGTAGAAAAA 
CAATTCAAGG CATTTCGCAG AGGTTTTCAT ATGGTGACTA ATGAATCACC CTTAAAATAC 
TTATTCAGAC CAGAAGAAAT TGAATTACTT ATATGTGGAA GCCGGAATCT CGATTTCCAG 
GCACTAGAAG AAACTACAGA GTATGATGGT GGCTATACGA GAGAATCTGT TGTGATTAGG 
GAGTTCTGGG AAATTGTTCA TTCGTTTACA GATGAACAGA AAAGACTTTT CCTGCAGTTT 
ACAACAGGCA CAGACAGAGC ACCTGTTGGA GGACTCGGAA AATTGAAGAT GATTATAGCC 
AAAAATGGCC CAGACACAGA AAGGTTACCT ACATCTCATA CTTGCTTTAA TGTCCTTTTA 
CTTCCGGAAT ATTCAAGCAA AGAAAAACTT AAAGAGAGAT TGCTGAAGGC CATCACATAT 
GCCAAAGGAT TTGGCATGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757621.1
CDS	2729..5332
Translation	MRILKYTTILRMRKLRHRKAKKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEEFKEVDYLTEEKVYEIYEFCREREDYSPLIRVIGRIFSSAEALVQSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDIDAIRRVYSSLLSDEKIETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETEIGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDEATRLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFCDLGDSHPILYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVSLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_008759399.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759399.2

ORF Insert Sequence:

ATGAGAATTC TGAAATATAC CACCATCCTA AGGATGAGGA AGCTGAGACA CAGAAAGGCT 
AAGAAGCGAG CAGCTGCAAA GCATCTAATA GAGCGCTACT ACCATCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAATGAGTTC TGTGCTTCCT GTCCAACTTT TCTTCGTATG 
GATAACAATG CAGCAGCTAT TAAAGCTCTT GAGCTTTATA AAATTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAGAACTC AAAAGGTGCA 
TCTAACAACT CAGAGATAAA AATGAACAAG AAGGAAGAGT TTAAAGAGGT GGACTACCTA 
ACTGAAGAGA AAGTATATGA AATTTATGAA TTTTGTAGGG AGAGAGAGGA TTACTCCCCT 
TTAATTCGTG TAATTGGAAG AATATTTTCT AGTGCTGAGG CACTGGTTCA GAGCTTTCGG 
AAAGTCAAAC AGCACACTAA GGAGGAATTG AAATCTCTTC AAGAGAAGGA TGAAGACAAG 
GATGAAGATG AAAAGGAAAA AGCTGCGTGT TCTGCTGCTG CTATGGAAGA AGACTCAGAA 
GCATCTTCTT CAAGGATGGG TGATAGCTCA CAGGGAGACA ACAATGTACA AAAATTAGGT 
CCTGATGATG TGACTGTGGA TATTGATGCT ATAAGAAGGG TCTACAGCAG TTTGCTCTCT 
GATGAAAAAA TAGAAACTGC CTTCCTGAAT GCACTTGTAT ATCTGTCACC TAACGTGGAA 
TGTGATTTGA CATATCATAA TGTGTACACT CGAGACCCTA ATTATCTCAA TTTGTTCATT 
ATTGTAATGG AAAATAGGAA TCTCCACAGT CCTGAATATC TGGAAATGGC ATTGCCGTTA 
TTTTGCAAAG CTATGAGTAA GCTACCCCTT GCAGCTCAAG GAAAACTGAT TAGGCTGTGG 
TCTAAATACA GTGCTGACCA GATTCGGAGA ATGATGGAAA CATTTCAGCA ACTTATTACC 
TACAAAGTCA TAAGCAATGA ATTTAATAGC CGAAATCTAG TGAATGATGA TGATGCCATT 
GTTGCTGCTT CAAAGTGTTT GAAAATGGTT TACTATGCAA ATGTAGTGGG AGGGGACGTG 
GACACAAATC ATAATGAGGA AGATGATGAA GAGCCTATAC CTGAGTCCAG TGAATTGACA 
CTTCAGGAGC TTCTGGGAGA GGAAAGAAGA AATAAGAAAG GTCCTCGAGT GGATCCACTA 
GAAACCGAAA TTGGCGTTAA AACTCTAGAT TGTCGAAAAC CACTTATCTC CTTTGAAGAA 
TTCATTAATG AACCACTGAA TGATGTTCTA GAAATGGACA AAGATTATAC TTTTTTCAAA 
GTTGAAACAG AGAACAAATT CTCTTTTATG ACATGTCCCT TTATATTGAA TGCTGTCACA 
AAGAATCTGG GATTGTATTA TGACAATAGA ATTCGCATGT ACAGTGAAAG AAGAATCACT 
GTTCTTTACA GCCTAGTTCA AGGACAGCAG TTGAATCCAT ATTTGAGACT TAAAGTCAGA 
CGTGACCATA TTATAGATGA TGCACTTGTC CGGCTAGAGA TGATCGCTAT GGAAAATCCT 
GCAGACTTGA AGAAACAGTT GTATGTGGAA TTTGAAGGAG AACAAGGAGT AGATGAGGGA 
GGCGTTTCCA AAGAGTTTTT TCAGTTGGTT GTGGAGGAAA TCTTTAATCC AGATATTGGT 
ATGTTCACAT ACGATGAAGC TACAAGATTA TTTTGGTTTA ATCCATCTTC TTTTGAAACT 
GAGGGTCAAT TTACTCTGAT TGGCATAGTC CTGGGTCTGG CTATTTACAA TAACTGTATA 
CTGGATGTCC ATTTTCCCAT GGTTGTGTAC AGGAAGCTAA TGGGGAAAAA AGGAACGTTT 
TGTGACTTGG GAGACTCTCA CCCAATCTTA TATCAGAGTT TAAAGGATTT ATTGGAATAT 
GAAGGGAATG TGGAAGATGA TATGATGATC ACTTTCCAGA TATCACAGAC AGATCTTTTT 
GGTAACCCAA TGATGTATGA TCTAAAAGAA AATGGTGATA AAATTCCAAT TACAAATGAA 
AACAGGAAGG AATTTGTCAG TCTCTATTCA GACTACATTC TCAATAAATC TGTAGAAAAA 
CAATTCAAGG CATTTCGCAG AGGTTTTCAT ATGGTGACTA ATGAATCACC CTTAAAATAC 
TTATTCAGAC CAGAAGAAAT TGAATTACTT ATATGTGGAA GCCGGAATCT CGATTTCCAG 
GCACTAGAAG AAACTACAGA GTATGATGGT GGCTATACGA GAGAATCTGT TGTGATTAGG 
GAGTTCTGGG AAATTGTTCA TTCGTTTACA GATGAACAGA AAAGACTTTT CCTGCAGTTT 
ACAACAGGCA CAGACAGAGC ACCTGTTGGA GGACTCGGAA AATTGAAGAT GATTATAGCC 
AAAAATGGCC CAGACACAGA AAGGTTACCT ACATCTCATA CTTGCTTTAA TGTCCTTTTA 
CTTCCGGAAT ATTCAAGCAA AGAAAAACTT AAAGAGAGAT TGCTGAAGGC CATCACATAT 
GCCAAAGGAT TTGGCATGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube3a ( XM_008759401.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757623.1 Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa22859
Clone ID Related Accession (Same CDS sequence)	XM_008759401.2 , XM_006229313.3 , XM_008759400.2
Accession Version	XM_008759401.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-protein ligase E3A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAGCGCTACT ACCATCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAATGAGTTC TGTGCTTCCT GTCCAACTTT TCTTCGTATG 
GATAACAATG CAGCAGCTAT TAAAGCTCTT GAGCTTTATA AAATTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAGAACTC AAAAGGTGCA 
TCTAACAACT CAGAGATAAA AATGAACAAG AAGGAAGAGT TTAAAGAGGT GGACTACCTA 
ACTGAAGAGA AAGTATATGA AATTTATGAA TTTTGTAGGG AGAGAGAGGA TTACTCCCCT 
TTAATTCGTG TAATTGGAAG AATATTTTCT AGTGCTGAGG CACTGGTTCA GAGCTTTCGG 
AAAGTCAAAC AGCACACTAA GGAGGAATTG AAATCTCTTC AAGAGAAGGA TGAAGACAAG 
GATGAAGATG AAAAGGAAAA AGCTGCGTGT TCTGCTGCTG CTATGGAAGA AGACTCAGAA 
GCATCTTCTT CAAGGATGGG TGATAGCTCA CAGGGAGACA ACAATGTACA AAAATTAGGT 
CCTGATGATG TGACTGTGGA TATTGATGCT ATAAGAAGGG TCTACAGCAG TTTGCTCTCT 
GATGAAAAAA TAGAAACTGC CTTCCTGAAT GCACTTGTAT ATCTGTCACC TAACGTGGAA 
TGTGATTTGA CATATCATAA TGTGTACACT CGAGACCCTA ATTATCTCAA TTTGTTCATT 
ATTGTAATGG AAAATAGGAA TCTCCACAGT CCTGAATATC TGGAAATGGC ATTGCCGTTA 
TTTTGCAAAG CTATGAGTAA GCTACCCCTT GCAGCTCAAG GAAAACTGAT TAGGCTGTGG 
TCTAAATACA GTGCTGACCA GATTCGGAGA ATGATGGAAA CATTTCAGCA ACTTATTACC 
TACAAAGTCA TAAGCAATGA ATTTAATAGC CGAAATCTAG TGAATGATGA TGATGCCATT 
GTTGCTGCTT CAAAGTGTTT GAAAATGGTT TACTATGCAA ATGTAGTGGG AGGGGACGTG 
GACACAAATC ATAATGAGGA AGATGATGAA GAGCCTATAC CTGAGTCCAG TGAATTGACA 
CTTCAGGAGC TTCTGGGAGA GGAAAGAAGA AATAAGAAAG GTCCTCGAGT GGATCCACTA 
GAAACCGAAA TTGGCGTTAA AACTCTAGAT TGTCGAAAAC CACTTATCTC CTTTGAAGAA 
TTCATTAATG AACCACTGAA TGATGTTCTA GAAATGGACA AAGATTATAC TTTTTTCAAA 
GTTGAAACAG AGAACAAATT CTCTTTTATG ACATGTCCCT TTATATTGAA TGCTGTCACA 
AAGAATCTGG GATTGTATTA TGACAATAGA ATTCGCATGT ACAGTGAAAG AAGAATCACT 
GTTCTTTACA GCCTAGTTCA AGGACAGCAG TTGAATCCAT ATTTGAGACT TAAAGTCAGA 
CGTGACCATA TTATAGATGA TGCACTTGTC CGGCTAGAGA TGATCGCTAT GGAAAATCCT 
GCAGACTTGA AGAAACAGTT GTATGTGGAA TTTGAAGGAG AACAAGGAGT AGATGAGGGA 
GGCGTTTCCA AAGAGTTTTT TCAGTTGGTT GTGGAGGAAA TCTTTAATCC AGATATTGGT 
ATGTTCACAT ACGATGAAGC TACAAGATTA TTTTGGTTTA ATCCATCTTC TTTTGAAACT 
GAGGGTCAAT TTACTCTGAT TGGCATAGTC CTGGGTCTGG CTATTTACAA TAACTGTATA 
CTGGATGTCC ATTTTCCCAT GGTTGTGTAC AGGAAGCTAA TGGGGAAAAA AGGAACGTTT 
TGTGACTTGG GAGACTCTCA CCCAATCTTA TATCAGAGTT TAAAGGATTT ATTGGAATAT 
GAAGGGAATG TGGAAGATGA TATGATGATC ACTTTCCAGA TATCACAGAC AGATCTTTTT 
GGTAACCCAA TGATGTATGA TCTAAAAGAA AATGGTGATA AAATTCCAAT TACAAATGAA 
AACAGGAAGG AATTTGTCAG TCTCTATTCA GACTACATTC TCAATAAATC TGTAGAAAAA 
CAATTCAAGG CATTTCGCAG AGGTTTTCAT ATGGTGACTA ATGAATCACC CTTAAAATAC 
TTATTCAGAC CAGAAGAAAT TGAATTACTT ATATGTGGAA GCCGGAATCT CGATTTCCAG 
GCACTAGAAG AAACTACAGA GTATGATGGT GGCTATACGA GAGAATCTGT TGTGATTAGG 
GAGTTCTGGG AAATTGTTCA TTCGTTTACA GATGAACAGA AAAGACTTTT CCTGCAGTTT 
ACAACAGGCA CAGACAGAGC ACCTGTTGGA GGACTCGGAA AATTGAAGAT GATTATAGCC 
AAAAATGGCC CAGACACAGA AAGGTTACCT ACATCTCATA CTTGCTTTAA TGTCCTTTTA 
CTTCCGGAAT ATTCAAGCAA AGAAAAACTT AAAGAGAGAT TGCTGAAGGC CATCACATAT 
GCCAAAGGAT TTGGCATGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757623.1
CDS	1528..4071
Translation	MKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEEFKEVDYLTEEKVYEIYEFCREREDYSPLIRVIGRIFSSAEALVQSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDIDAIRRVYSSLLSDEKIETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETEIGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDEATRLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFCDLGDSHPILYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVSLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_008759401.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759401.2

ORF Insert Sequence:

ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAGCGCTACT ACCATCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAATGAGTTC TGTGCTTCCT GTCCAACTTT TCTTCGTATG 
GATAACAATG CAGCAGCTAT TAAAGCTCTT GAGCTTTATA AAATTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAGAACTC AAAAGGTGCA 
TCTAACAACT CAGAGATAAA AATGAACAAG AAGGAAGAGT TTAAAGAGGT GGACTACCTA 
ACTGAAGAGA AAGTATATGA AATTTATGAA TTTTGTAGGG AGAGAGAGGA TTACTCCCCT 
TTAATTCGTG TAATTGGAAG AATATTTTCT AGTGCTGAGG CACTGGTTCA GAGCTTTCGG 
AAAGTCAAAC AGCACACTAA GGAGGAATTG AAATCTCTTC AAGAGAAGGA TGAAGACAAG 
GATGAAGATG AAAAGGAAAA AGCTGCGTGT TCTGCTGCTG CTATGGAAGA AGACTCAGAA 
GCATCTTCTT CAAGGATGGG TGATAGCTCA CAGGGAGACA ACAATGTACA AAAATTAGGT 
CCTGATGATG TGACTGTGGA TATTGATGCT ATAAGAAGGG TCTACAGCAG TTTGCTCTCT 
GATGAAAAAA TAGAAACTGC CTTCCTGAAT GCACTTGTAT ATCTGTCACC TAACGTGGAA 
TGTGATTTGA CATATCATAA TGTGTACACT CGAGACCCTA ATTATCTCAA TTTGTTCATT 
ATTGTAATGG AAAATAGGAA TCTCCACAGT CCTGAATATC TGGAAATGGC ATTGCCGTTA 
TTTTGCAAAG CTATGAGTAA GCTACCCCTT GCAGCTCAAG GAAAACTGAT TAGGCTGTGG 
TCTAAATACA GTGCTGACCA GATTCGGAGA ATGATGGAAA CATTTCAGCA ACTTATTACC 
TACAAAGTCA TAAGCAATGA ATTTAATAGC CGAAATCTAG TGAATGATGA TGATGCCATT 
GTTGCTGCTT CAAAGTGTTT GAAAATGGTT TACTATGCAA ATGTAGTGGG AGGGGACGTG 
GACACAAATC ATAATGAGGA AGATGATGAA GAGCCTATAC CTGAGTCCAG TGAATTGACA 
CTTCAGGAGC TTCTGGGAGA GGAAAGAAGA AATAAGAAAG GTCCTCGAGT GGATCCACTA 
GAAACCGAAA TTGGCGTTAA AACTCTAGAT TGTCGAAAAC CACTTATCTC CTTTGAAGAA 
TTCATTAATG AACCACTGAA TGATGTTCTA GAAATGGACA AAGATTATAC TTTTTTCAAA 
GTTGAAACAG AGAACAAATT CTCTTTTATG ACATGTCCCT TTATATTGAA TGCTGTCACA 
AAGAATCTGG GATTGTATTA TGACAATAGA ATTCGCATGT ACAGTGAAAG AAGAATCACT 
GTTCTTTACA GCCTAGTTCA AGGACAGCAG TTGAATCCAT ATTTGAGACT TAAAGTCAGA 
CGTGACCATA TTATAGATGA TGCACTTGTC CGGCTAGAGA TGATCGCTAT GGAAAATCCT 
GCAGACTTGA AGAAACAGTT GTATGTGGAA TTTGAAGGAG AACAAGGAGT AGATGAGGGA 
GGCGTTTCCA AAGAGTTTTT TCAGTTGGTT GTGGAGGAAA TCTTTAATCC AGATATTGGT 
ATGTTCACAT ACGATGAAGC TACAAGATTA TTTTGGTTTA ATCCATCTTC TTTTGAAACT 
GAGGGTCAAT TTACTCTGAT TGGCATAGTC CTGGGTCTGG CTATTTACAA TAACTGTATA 
CTGGATGTCC ATTTTCCCAT GGTTGTGTAC AGGAAGCTAA TGGGGAAAAA AGGAACGTTT 
TGTGACTTGG GAGACTCTCA CCCAATCTTA TATCAGAGTT TAAAGGATTT ATTGGAATAT 
GAAGGGAATG TGGAAGATGA TATGATGATC ACTTTCCAGA TATCACAGAC AGATCTTTTT 
GGTAACCCAA TGATGTATGA TCTAAAAGAA AATGGTGATA AAATTCCAAT TACAAATGAA 
AACAGGAAGG AATTTGTCAG TCTCTATTCA GACTACATTC TCAATAAATC TGTAGAAAAA 
CAATTCAAGG CATTTCGCAG AGGTTTTCAT ATGGTGACTA ATGAATCACC CTTAAAATAC 
TTATTCAGAC CAGAAGAAAT TGAATTACTT ATATGTGGAA GCCGGAATCT CGATTTCCAG 
GCACTAGAAG AAACTACAGA GTATGATGGT GGCTATACGA GAGAATCTGT TGTGATTAGG 
GAGTTCTGGG AAATTGTTCA TTCGTTTACA GATGAACAGA AAAGACTTTT CCTGCAGTTT 
ACAACAGGCA CAGACAGAGC ACCTGTTGGA GGACTCGGAA AATTGAAGAT GATTATAGCC 
AAAAATGGCC CAGACACAGA AAGGTTACCT ACATCTCATA CTTGCTTTAA TGTCCTTTTA 
CTTCCGGAAT ATTCAAGCAA AGAAAAACTT AAAGAGAGAT TGCTGAAGGC CATCACATAT 
GCCAAAGGAT TTGGCATGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube3a ( XM_006229313.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229375.1 Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa22859
Clone ID Related Accession (Same CDS sequence)	XM_008759401.2 , XM_006229313.3 , XM_008759400.2
Accession Version	XM_006229313.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-protein ligase E3A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229313.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAGCGCTACT ACCATCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAATGAGTTC TGTGCTTCCT GTCCAACTTT TCTTCGTATG 
GATAACAATG CAGCAGCTAT TAAAGCTCTT GAGCTTTATA AAATTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAGAACTC AAAAGGTGCA 
TCTAACAACT CAGAGATAAA AATGAACAAG AAGGAAGAGT TTAAAGAGGT GGACTACCTA 
ACTGAAGAGA AAGTATATGA AATTTATGAA TTTTGTAGGG AGAGAGAGGA TTACTCCCCT 
TTAATTCGTG TAATTGGAAG AATATTTTCT AGTGCTGAGG CACTGGTTCA GAGCTTTCGG 
AAAGTCAAAC AGCACACTAA GGAGGAATTG AAATCTCTTC AAGAGAAGGA TGAAGACAAG 
GATGAAGATG AAAAGGAAAA AGCTGCGTGT TCTGCTGCTG CTATGGAAGA AGACTCAGAA 
GCATCTTCTT CAAGGATGGG TGATAGCTCA CAGGGAGACA ACAATGTACA AAAATTAGGT 
CCTGATGATG TGACTGTGGA TATTGATGCT ATAAGAAGGG TCTACAGCAG TTTGCTCTCT 
GATGAAAAAA TAGAAACTGC CTTCCTGAAT GCACTTGTAT ATCTGTCACC TAACGTGGAA 
TGTGATTTGA CATATCATAA TGTGTACACT CGAGACCCTA ATTATCTCAA TTTGTTCATT 
ATTGTAATGG AAAATAGGAA TCTCCACAGT CCTGAATATC TGGAAATGGC ATTGCCGTTA 
TTTTGCAAAG CTATGAGTAA GCTACCCCTT GCAGCTCAAG GAAAACTGAT TAGGCTGTGG 
TCTAAATACA GTGCTGACCA GATTCGGAGA ATGATGGAAA CATTTCAGCA ACTTATTACC 
TACAAAGTCA TAAGCAATGA ATTTAATAGC CGAAATCTAG TGAATGATGA TGATGCCATT 
GTTGCTGCTT CAAAGTGTTT GAAAATGGTT TACTATGCAA ATGTAGTGGG AGGGGACGTG 
GACACAAATC ATAATGAGGA AGATGATGAA GAGCCTATAC CTGAGTCCAG TGAATTGACA 
CTTCAGGAGC TTCTGGGAGA GGAAAGAAGA AATAAGAAAG GTCCTCGAGT GGATCCACTA 
GAAACCGAAA TTGGCGTTAA AACTCTAGAT TGTCGAAAAC CACTTATCTC CTTTGAAGAA 
TTCATTAATG AACCACTGAA TGATGTTCTA GAAATGGACA AAGATTATAC TTTTTTCAAA 
GTTGAAACAG AGAACAAATT CTCTTTTATG ACATGTCCCT TTATATTGAA TGCTGTCACA 
AAGAATCTGG GATTGTATTA TGACAATAGA ATTCGCATGT ACAGTGAAAG AAGAATCACT 
GTTCTTTACA GCCTAGTTCA AGGACAGCAG TTGAATCCAT ATTTGAGACT TAAAGTCAGA 
CGTGACCATA TTATAGATGA TGCACTTGTC CGGCTAGAGA TGATCGCTAT GGAAAATCCT 
GCAGACTTGA AGAAACAGTT GTATGTGGAA TTTGAAGGAG AACAAGGAGT AGATGAGGGA 
GGCGTTTCCA AAGAGTTTTT TCAGTTGGTT GTGGAGGAAA TCTTTAATCC AGATATTGGT 
ATGTTCACAT ACGATGAAGC TACAAGATTA TTTTGGTTTA ATCCATCTTC TTTTGAAACT 
GAGGGTCAAT TTACTCTGAT TGGCATAGTC CTGGGTCTGG CTATTTACAA TAACTGTATA 
CTGGATGTCC ATTTTCCCAT GGTTGTGTAC AGGAAGCTAA TGGGGAAAAA AGGAACGTTT 
TGTGACTTGG GAGACTCTCA CCCAATCTTA TATCAGAGTT TAAAGGATTT ATTGGAATAT 
GAAGGGAATG TGGAAGATGA TATGATGATC ACTTTCCAGA TATCACAGAC AGATCTTTTT 
GGTAACCCAA TGATGTATGA TCTAAAAGAA AATGGTGATA AAATTCCAAT TACAAATGAA 
AACAGGAAGG AATTTGTCAG TCTCTATTCA GACTACATTC TCAATAAATC TGTAGAAAAA 
CAATTCAAGG CATTTCGCAG AGGTTTTCAT ATGGTGACTA ATGAATCACC CTTAAAATAC 
TTATTCAGAC CAGAAGAAAT TGAATTACTT ATATGTGGAA GCCGGAATCT CGATTTCCAG 
GCACTAGAAG AAACTACAGA GTATGATGGT GGCTATACGA GAGAATCTGT TGTGATTAGG 
GAGTTCTGGG AAATTGTTCA TTCGTTTACA GATGAACAGA AAAGACTTTT CCTGCAGTTT 
ACAACAGGCA CAGACAGAGC ACCTGTTGGA GGACTCGGAA AATTGAAGAT GATTATAGCC 
AAAAATGGCC CAGACACAGA AAGGTTACCT ACATCTCATA CTTGCTTTAA TGTCCTTTTA 
CTTCCGGAAT ATTCAAGCAA AGAAAAACTT AAAGAGAGAT TGCTGAAGGC CATCACATAT 
GCCAAAGGAT TTGGCATGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229375.1
CDS	1464..4007
Translation	MKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEEFKEVDYLTEEKVYEIYEFCREREDYSPLIRVIGRIFSSAEALVQSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDIDAIRRVYSSLLSDEKIETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETEIGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDEATRLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFCDLGDSHPILYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVSLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_006229313.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229313.3

ORF Insert Sequence:

ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAGCGCTACT ACCATCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAATGAGTTC TGTGCTTCCT GTCCAACTTT TCTTCGTATG 
GATAACAATG CAGCAGCTAT TAAAGCTCTT GAGCTTTATA AAATTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAGAACTC AAAAGGTGCA 
TCTAACAACT CAGAGATAAA AATGAACAAG AAGGAAGAGT TTAAAGAGGT GGACTACCTA 
ACTGAAGAGA AAGTATATGA AATTTATGAA TTTTGTAGGG AGAGAGAGGA TTACTCCCCT 
TTAATTCGTG TAATTGGAAG AATATTTTCT AGTGCTGAGG CACTGGTTCA GAGCTTTCGG 
AAAGTCAAAC AGCACACTAA GGAGGAATTG AAATCTCTTC AAGAGAAGGA TGAAGACAAG 
GATGAAGATG AAAAGGAAAA AGCTGCGTGT TCTGCTGCTG CTATGGAAGA AGACTCAGAA 
GCATCTTCTT CAAGGATGGG TGATAGCTCA CAGGGAGACA ACAATGTACA AAAATTAGGT 
CCTGATGATG TGACTGTGGA TATTGATGCT ATAAGAAGGG TCTACAGCAG TTTGCTCTCT 
GATGAAAAAA TAGAAACTGC CTTCCTGAAT GCACTTGTAT ATCTGTCACC TAACGTGGAA 
TGTGATTTGA CATATCATAA TGTGTACACT CGAGACCCTA ATTATCTCAA TTTGTTCATT 
ATTGTAATGG AAAATAGGAA TCTCCACAGT CCTGAATATC TGGAAATGGC ATTGCCGTTA 
TTTTGCAAAG CTATGAGTAA GCTACCCCTT GCAGCTCAAG GAAAACTGAT TAGGCTGTGG 
TCTAAATACA GTGCTGACCA GATTCGGAGA ATGATGGAAA CATTTCAGCA ACTTATTACC 
TACAAAGTCA TAAGCAATGA ATTTAATAGC CGAAATCTAG TGAATGATGA TGATGCCATT 
GTTGCTGCTT CAAAGTGTTT GAAAATGGTT TACTATGCAA ATGTAGTGGG AGGGGACGTG 
GACACAAATC ATAATGAGGA AGATGATGAA GAGCCTATAC CTGAGTCCAG TGAATTGACA 
CTTCAGGAGC TTCTGGGAGA GGAAAGAAGA AATAAGAAAG GTCCTCGAGT GGATCCACTA 
GAAACCGAAA TTGGCGTTAA AACTCTAGAT TGTCGAAAAC CACTTATCTC CTTTGAAGAA 
TTCATTAATG AACCACTGAA TGATGTTCTA GAAATGGACA AAGATTATAC TTTTTTCAAA 
GTTGAAACAG AGAACAAATT CTCTTTTATG ACATGTCCCT TTATATTGAA TGCTGTCACA 
AAGAATCTGG GATTGTATTA TGACAATAGA ATTCGCATGT ACAGTGAAAG AAGAATCACT 
GTTCTTTACA GCCTAGTTCA AGGACAGCAG TTGAATCCAT ATTTGAGACT TAAAGTCAGA 
CGTGACCATA TTATAGATGA TGCACTTGTC CGGCTAGAGA TGATCGCTAT GGAAAATCCT 
GCAGACTTGA AGAAACAGTT GTATGTGGAA TTTGAAGGAG AACAAGGAGT AGATGAGGGA 
GGCGTTTCCA AAGAGTTTTT TCAGTTGGTT GTGGAGGAAA TCTTTAATCC AGATATTGGT 
ATGTTCACAT ACGATGAAGC TACAAGATTA TTTTGGTTTA ATCCATCTTC TTTTGAAACT 
GAGGGTCAAT TTACTCTGAT TGGCATAGTC CTGGGTCTGG CTATTTACAA TAACTGTATA 
CTGGATGTCC ATTTTCCCAT GGTTGTGTAC AGGAAGCTAA TGGGGAAAAA AGGAACGTTT 
TGTGACTTGG GAGACTCTCA CCCAATCTTA TATCAGAGTT TAAAGGATTT ATTGGAATAT 
GAAGGGAATG TGGAAGATGA TATGATGATC ACTTTCCAGA TATCACAGAC AGATCTTTTT 
GGTAACCCAA TGATGTATGA TCTAAAAGAA AATGGTGATA AAATTCCAAT TACAAATGAA 
AACAGGAAGG AATTTGTCAG TCTCTATTCA GACTACATTC TCAATAAATC TGTAGAAAAA 
CAATTCAAGG CATTTCGCAG AGGTTTTCAT ATGGTGACTA ATGAATCACC CTTAAAATAC 
TTATTCAGAC CAGAAGAAAT TGAATTACTT ATATGTGGAA GCCGGAATCT CGATTTCCAG 
GCACTAGAAG AAACTACAGA GTATGATGGT GGCTATACGA GAGAATCTGT TGTGATTAGG 
GAGTTCTGGG AAATTGTTCA TTCGTTTACA GATGAACAGA AAAGACTTTT CCTGCAGTTT 
ACAACAGGCA CAGACAGAGC ACCTGTTGGA GGACTCGGAA AATTGAAGAT GATTATAGCC 
AAAAATGGCC CAGACACAGA AAGGTTACCT ACATCTCATA CTTGCTTTAA TGTCCTTTTA 
CTTCCGGAAT ATTCAAGCAA AGAAAAACTT AAAGAGAGAT TGCTGAAGGC CATCACATAT 
GCCAAAGGAT TTGGCATGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube3a ( XM_008759400.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757622.1 Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa22859
Clone ID Related Accession (Same CDS sequence)	XM_008759401.2 , XM_006229313.3 , XM_008759400.2
Accession Version	XM_008759400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-protein ligase E3A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAGCGCTACT ACCATCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAATGAGTTC TGTGCTTCCT GTCCAACTTT TCTTCGTATG 
GATAACAATG CAGCAGCTAT TAAAGCTCTT GAGCTTTATA AAATTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAGAACTC AAAAGGTGCA 
TCTAACAACT CAGAGATAAA AATGAACAAG AAGGAAGAGT TTAAAGAGGT GGACTACCTA 
ACTGAAGAGA AAGTATATGA AATTTATGAA TTTTGTAGGG AGAGAGAGGA TTACTCCCCT 
TTAATTCGTG TAATTGGAAG AATATTTTCT AGTGCTGAGG CACTGGTTCA GAGCTTTCGG 
AAAGTCAAAC AGCACACTAA GGAGGAATTG AAATCTCTTC AAGAGAAGGA TGAAGACAAG 
GATGAAGATG AAAAGGAAAA AGCTGCGTGT TCTGCTGCTG CTATGGAAGA AGACTCAGAA 
GCATCTTCTT CAAGGATGGG TGATAGCTCA CAGGGAGACA ACAATGTACA AAAATTAGGT 
CCTGATGATG TGACTGTGGA TATTGATGCT ATAAGAAGGG TCTACAGCAG TTTGCTCTCT 
GATGAAAAAA TAGAAACTGC CTTCCTGAAT GCACTTGTAT ATCTGTCACC TAACGTGGAA 
TGTGATTTGA CATATCATAA TGTGTACACT CGAGACCCTA ATTATCTCAA TTTGTTCATT 
ATTGTAATGG AAAATAGGAA TCTCCACAGT CCTGAATATC TGGAAATGGC ATTGCCGTTA 
TTTTGCAAAG CTATGAGTAA GCTACCCCTT GCAGCTCAAG GAAAACTGAT TAGGCTGTGG 
TCTAAATACA GTGCTGACCA GATTCGGAGA ATGATGGAAA CATTTCAGCA ACTTATTACC 
TACAAAGTCA TAAGCAATGA ATTTAATAGC CGAAATCTAG TGAATGATGA TGATGCCATT 
GTTGCTGCTT CAAAGTGTTT GAAAATGGTT TACTATGCAA ATGTAGTGGG AGGGGACGTG 
GACACAAATC ATAATGAGGA AGATGATGAA GAGCCTATAC CTGAGTCCAG TGAATTGACA 
CTTCAGGAGC TTCTGGGAGA GGAAAGAAGA AATAAGAAAG GTCCTCGAGT GGATCCACTA 
GAAACCGAAA TTGGCGTTAA AACTCTAGAT TGTCGAAAAC CACTTATCTC CTTTGAAGAA 
TTCATTAATG AACCACTGAA TGATGTTCTA GAAATGGACA AAGATTATAC TTTTTTCAAA 
GTTGAAACAG AGAACAAATT CTCTTTTATG ACATGTCCCT TTATATTGAA TGCTGTCACA 
AAGAATCTGG GATTGTATTA TGACAATAGA ATTCGCATGT ACAGTGAAAG AAGAATCACT 
GTTCTTTACA GCCTAGTTCA AGGACAGCAG TTGAATCCAT ATTTGAGACT TAAAGTCAGA 
CGTGACCATA TTATAGATGA TGCACTTGTC CGGCTAGAGA TGATCGCTAT GGAAAATCCT 
GCAGACTTGA AGAAACAGTT GTATGTGGAA TTTGAAGGAG AACAAGGAGT AGATGAGGGA 
GGCGTTTCCA AAGAGTTTTT TCAGTTGGTT GTGGAGGAAA TCTTTAATCC AGATATTGGT 
ATGTTCACAT ACGATGAAGC TACAAGATTA TTTTGGTTTA ATCCATCTTC TTTTGAAACT 
GAGGGTCAAT TTACTCTGAT TGGCATAGTC CTGGGTCTGG CTATTTACAA TAACTGTATA 
CTGGATGTCC ATTTTCCCAT GGTTGTGTAC AGGAAGCTAA TGGGGAAAAA AGGAACGTTT 
TGTGACTTGG GAGACTCTCA CCCAATCTTA TATCAGAGTT TAAAGGATTT ATTGGAATAT 
GAAGGGAATG TGGAAGATGA TATGATGATC ACTTTCCAGA TATCACAGAC AGATCTTTTT 
GGTAACCCAA TGATGTATGA TCTAAAAGAA AATGGTGATA AAATTCCAAT TACAAATGAA 
AACAGGAAGG AATTTGTCAG TCTCTATTCA GACTACATTC TCAATAAATC TGTAGAAAAA 
CAATTCAAGG CATTTCGCAG AGGTTTTCAT ATGGTGACTA ATGAATCACC CTTAAAATAC 
TTATTCAGAC CAGAAGAAAT TGAATTACTT ATATGTGGAA GCCGGAATCT CGATTTCCAG 
GCACTAGAAG AAACTACAGA GTATGATGGT GGCTATACGA GAGAATCTGT TGTGATTAGG 
GAGTTCTGGG AAATTGTTCA TTCGTTTACA GATGAACAGA AAAGACTTTT CCTGCAGTTT 
ACAACAGGCA CAGACAGAGC ACCTGTTGGA GGACTCGGAA AATTGAAGAT GATTATAGCC 
AAAAATGGCC CAGACACAGA AAGGTTACCT ACATCTCATA CTTGCTTTAA TGTCCTTTTA 
CTTCCGGAAT ATTCAAGCAA AGAAAAACTT AAAGAGAGAT TGCTGAAGGC CATCACATAT 
GCCAAAGGAT TTGGCATGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757622.1
CDS	1640..4183
Translation	MKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEEFKEVDYLTEEKVYEIYEFCREREDYSPLIRVIGRIFSSAEALVQSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDIDAIRRVYSSLLSDEKIETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETEIGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDEATRLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFCDLGDSHPILYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVSLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_008759400.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin protein ligase E3A (Ube3a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759400.2

ORF Insert Sequence:

ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAGCGCTACT ACCATCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAATGAGTTC TGTGCTTCCT GTCCAACTTT TCTTCGTATG 
GATAACAATG CAGCAGCTAT TAAAGCTCTT GAGCTTTATA AAATTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAGAACTC AAAAGGTGCA 
TCTAACAACT CAGAGATAAA AATGAACAAG AAGGAAGAGT TTAAAGAGGT GGACTACCTA 
ACTGAAGAGA AAGTATATGA AATTTATGAA TTTTGTAGGG AGAGAGAGGA TTACTCCCCT 
TTAATTCGTG TAATTGGAAG AATATTTTCT AGTGCTGAGG CACTGGTTCA GAGCTTTCGG 
AAAGTCAAAC AGCACACTAA GGAGGAATTG AAATCTCTTC AAGAGAAGGA TGAAGACAAG 
GATGAAGATG AAAAGGAAAA AGCTGCGTGT TCTGCTGCTG CTATGGAAGA AGACTCAGAA 
GCATCTTCTT CAAGGATGGG TGATAGCTCA CAGGGAGACA ACAATGTACA AAAATTAGGT 
CCTGATGATG TGACTGTGGA TATTGATGCT ATAAGAAGGG TCTACAGCAG TTTGCTCTCT 
GATGAAAAAA TAGAAACTGC CTTCCTGAAT GCACTTGTAT ATCTGTCACC TAACGTGGAA 
TGTGATTTGA CATATCATAA TGTGTACACT CGAGACCCTA ATTATCTCAA TTTGTTCATT 
ATTGTAATGG AAAATAGGAA TCTCCACAGT CCTGAATATC TGGAAATGGC ATTGCCGTTA 
TTTTGCAAAG CTATGAGTAA GCTACCCCTT GCAGCTCAAG GAAAACTGAT TAGGCTGTGG 
TCTAAATACA GTGCTGACCA GATTCGGAGA ATGATGGAAA CATTTCAGCA ACTTATTACC 
TACAAAGTCA TAAGCAATGA ATTTAATAGC CGAAATCTAG TGAATGATGA TGATGCCATT 
GTTGCTGCTT CAAAGTGTTT GAAAATGGTT TACTATGCAA ATGTAGTGGG AGGGGACGTG 
GACACAAATC ATAATGAGGA AGATGATGAA GAGCCTATAC CTGAGTCCAG TGAATTGACA 
CTTCAGGAGC TTCTGGGAGA GGAAAGAAGA AATAAGAAAG GTCCTCGAGT GGATCCACTA 
GAAACCGAAA TTGGCGTTAA AACTCTAGAT TGTCGAAAAC CACTTATCTC CTTTGAAGAA 
TTCATTAATG AACCACTGAA TGATGTTCTA GAAATGGACA AAGATTATAC TTTTTTCAAA 
GTTGAAACAG AGAACAAATT CTCTTTTATG ACATGTCCCT TTATATTGAA TGCTGTCACA 
AAGAATCTGG GATTGTATTA TGACAATAGA ATTCGCATGT ACAGTGAAAG AAGAATCACT 
GTTCTTTACA GCCTAGTTCA AGGACAGCAG TTGAATCCAT ATTTGAGACT TAAAGTCAGA 
CGTGACCATA TTATAGATGA TGCACTTGTC CGGCTAGAGA TGATCGCTAT GGAAAATCCT 
GCAGACTTGA AGAAACAGTT GTATGTGGAA TTTGAAGGAG AACAAGGAGT AGATGAGGGA 
GGCGTTTCCA AAGAGTTTTT TCAGTTGGTT GTGGAGGAAA TCTTTAATCC AGATATTGGT 
ATGTTCACAT ACGATGAAGC TACAAGATTA TTTTGGTTTA ATCCATCTTC TTTTGAAACT 
GAGGGTCAAT TTACTCTGAT TGGCATAGTC CTGGGTCTGG CTATTTACAA TAACTGTATA 
CTGGATGTCC ATTTTCCCAT GGTTGTGTAC AGGAAGCTAA TGGGGAAAAA AGGAACGTTT 
TGTGACTTGG GAGACTCTCA CCCAATCTTA TATCAGAGTT TAAAGGATTT ATTGGAATAT 
GAAGGGAATG TGGAAGATGA TATGATGATC ACTTTCCAGA TATCACAGAC AGATCTTTTT 
GGTAACCCAA TGATGTATGA TCTAAAAGAA AATGGTGATA AAATTCCAAT TACAAATGAA 
AACAGGAAGG AATTTGTCAG TCTCTATTCA GACTACATTC TCAATAAATC TGTAGAAAAA 
CAATTCAAGG CATTTCGCAG AGGTTTTCAT ATGGTGACTA ATGAATCACC CTTAAAATAC 
TTATTCAGAC CAGAAGAAAT TGAATTACTT ATATGTGGAA GCCGGAATCT CGATTTCCAG 
GCACTAGAAG AAACTACAGA GTATGATGGT GGCTATACGA GAGAATCTGT TGTGATTAGG 
GAGTTCTGGG AAATTGTTCA TTCGTTTACA GATGAACAGA AAAGACTTTT CCTGCAGTTT 
ACAACAGGCA CAGACAGAGC ACCTGTTGGA GGACTCGGAA AATTGAAGAT GATTATAGCC 
AAAAATGGCC CAGACACAGA AAGGTTACCT ACATCTCATA CTTGCTTTAA TGTCCTTTTA 
CTTCCGGAAT ATTCAAGCAA AGAAAAACTT AAAGAGAGAT TGCTGAAGGC CATCACATAT 
GCCAAAGGAT TTGGCATGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ube3a reinstatement identifies distinct developmental windows in a murine Angelman syndrome model.
The Journal of clinical investigation125(5)2069-76(2015 May)
Silva-Santos S,van Woerden GM,Bruinsma CF,Mientjes E,Jolfaei MA,Distel B,Kushner SA,Elgersma Y

A coding-independent function of an alternative Ube3a transcript during neuronal development.
Nature neuroscience18(5)666-73(2015 May)
Valluy J,Bicker S,Aksoy-Aksel A,Lackinger M,Sumer S,Fiore R,W?st T,Seffer D,Metge F,Dieterich C,W?hr M,Schwarting R,Schratt G

Towards a therapy for Angelman syndrome by targeting a long non-coding RNA.
Nature518(7539)409-12(2015 Feb)
Meng L,Ward AJ,Chun S,Bennett CF,Beaudet AL,Rigo F

Long-term abstinence from developmental cocaine exposure alters Arc/Arg3.1 modulation in the rat medial prefrontal cortex.
Neurotoxicity research26(3)299-306(2014 Oct)
Caffino L,Giannotti G,Malpighi C,Racagni G,Filip M,Fumagalli F

The E3 ubiquitin ligase UBE3A is an integral component of the molecular circadian clock through regulating the BMAL1 transcription factor.
Nucleic acids research42(9)5765-75(2014 May)
Gossan NC,Zhang F,Guo B,Jin D,Yoshitane H,Yao A,Glossop N,Zhang YQ,Fukada Y,Meng QJ

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Ube3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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