Dnaaf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnaaf1
Entrez Gene ID	361419
Full Name	dynein, axonemal, assembly factor 1
Synonyms	Lrrc50,RGD1310542
General protein information	Preferred Names dynein, axonemal, assembly factor 1 Names dynein assembly factor 1, axonemal leucine rich repeat containing 50 leucine-rich repeat-containing protein 50
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014154.1	NP_001014176.1	dynein assembly factor 1, axonemal
XM_017601323.1	XP_017456812.1	dynein assembly factor 1, axonemal isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dnaaf1	NP_080924.1
Homo sapiens (human)	DNAAF1	NP_848547.4
Pan troglodytes (chimpanzee)	LRRC50	XP_511137.2
Canis lupus familiaris (dog)	DNAAF1	XP_536764.3
Xenopus tropicalis (tropical clawed frog)	LOC101730543	XP_004913593.1
Rattus norvegicus (Norway rat)	Dnaaf1	NP_001014176.1
Bos taurus (cattle)	DNAAF1	NP_001030422.2

Gene Ontology
Bibliography

Related articles in PubMed

Deletions and point mutations of LRRC50 cause primary ciliary dyskinesia due to dynein arm defects.
Loges NT, Olbrich H, Becker-Heck A, H?ffner K, Heer A, Reinhard C, Schmidts M, Kispert A, Zariwala MA, Leigh MW, Knowles MR, Zentgraf H, Seithe H, N?rnberg G, N?rnberg P, Reinhardt R, Omran H
American journal of human genetics85(6)883-9(2009 Dec)

Loss-of-function mutations in the human ortholog of Chlamydomonas reinhardtii ODA7 disrupt dynein arm assembly and cause primary ciliary dyskinesia.
Duquesnoy P, Escudier E, Vincensini L, Freshour J, Bridoux AM, Coste A, Deschildre A, de Blic J, Legendre M, Montantin G, Tenreiro H, Vojtek AM, Loussert C, Cl?ment A, Escalier D, Bastin P, Mitchell DR, Amselem S
American journal of human genetics85(6)890-6(2009 Dec)

LRRC50, a conserved ciliary protein implicated in polycystic kidney disease.
van Rooijen E, Giles RH, Voest EE, van Rooijen C, Schulte-Merker S, van Eeden FJ
Journal of the American Society of Nephrology : JASN19(6)1128-38(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dnaaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnaaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05162	NM_001014154.1 Latest version!	Rattus norvegicus dynein, axonemal, assembly factor 1 (Dnaaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa59465	XM_017601323.1 Latest version!	Rattus norvegicus dynein, axonemal, assembly factor 1 (Dnaaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnaaf1 ( NM_001014154.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001014176.1 Rattus norvegicus dynein, axonemal, assembly factor 1 (Dnaaf1), mRNA.

Price & Availability↑

CloneID	ORa05162
Clone ID Related Accession (Same CDS sequence)	NM_001014154.1
Accession Version	NM_001014154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	dynein assembly factor 1, axonemal
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079038.1. On Apr 20, 2005 this sequence version replaced XM_341697.2. ##Evidence-Data-START## Transcript exon combination :: BC079038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCACCCCG AAGCCTCAGA GCCGCCGGTG GACAGCGCGG CGGAGCCCAG TCTGGAGGAG 
TCCGCAGGCG ATCACGGCGA CGCAGGCCCA GGGGTCCGCA AAGAAGAAAT TAATGAGACC 
AAGGAAACAT GTGTGGGTCC TTGTACAACA TCCTGCCAAA GTCAGCAGCA ACCGAGCGGT 
GACAATGGGT CAGATGGCCT CTTTACCCAC TCGAGAGATG ACAGAGATGA TCGGGGCCCC 
AGGATGACGA AGCAGTTCCT CCAAAAGCTC TGTAAGCAGC ACAAGCTGTA CGTCACCCCG 
GCCCTGAACG ACACCCTGTA TTTGCACTTT AAGGGCTTTG ACCGCATTGA GAACCTGGAG 
GAGTACACGG GCCTGCGCTG CCTCTGGCTG GAGTGCAACG GCATCCAGAG GATCGAGAAT 
TTGCAGGCCC AGAGCGAGCT GCGCTGCCTC TTCCTGCAAG TGAACCTGTT GCACAAGATC 
GAAAACCTGG AGCCGCTCCA GAAGCTGGAC GCCCTCAACC TGAGCAACAA CTACATCAAG 
ACCATTGAGA ACCTCTCCTG TCTGCCGGTC CTTAACACTC TGCAAATGGC CCACAACCGC 
CTGGAGACCG TGGCAGACAT TGAGCACCTC AGGGAGTGTC TGCAACTCTG TGTCCTCGAC 
CTCTCCCACA ACTCGCTGAG TGACCCCGAG ATCCTGAGCG TGCTTGAGAC CATGCCCTGT 
CTGCGTGTGC TGAACCTGAT GGGGAACCCA GTGACCAAAC ACATCCCTAA CTACCGCAGG 
ACGGTCACTG TCCGACTGAA GCACTTGACT TATCTGGACG ACAGGCCGGT TTTCCCCAAG 
GACAGGGCGT GTGCAGAGGC CTGGGCCCGA GGCGGGTATG CTGCCGAGAA GGAAGAGCGT 
CACCAGTGGG AGAGCAGGGA ACACAAGAAG ATAACAGACA GCCTGGAAGC CTTGGCCATG 
ATCAAGCGGC GGGCCGAGGA ACGGAAGAAG GCAAGAGACA GAGGGGAGAC ACCTCTGCCA 
GAGAGTGAAA AGAGTATCCC CACTAGCCCA GAGGCTCAGG AAAAGCCTCC CAAGGGGGAA 
ACCCAGCAGA AGATGGAGTC GTTTGTGAAA GAGAGCTTTG AGGCCAAAGA TGAACTGTTT 
CCAGAGAAGC CAGGAGAAGG AGAGGAGCTG TCTGTGGTGG TGGGGAATAG AGCAGTGGAA 
GATGCAGACC TGTCCGGGAA CCTGGCTCAC ACCCAGACAC CTGTGGTGGT CACCCCTGAA 
GAGGTCACAT CTCCAGTGGA AGCTACTGAT GGGGCCAGAA CGGAAGACAC AGAGGCCATT 
GCCCTGGAGA CCAAGGAGAA GCTTTTCATC GATGACCTGC CTGACTTGGA AGATGTTGAT 
GGTACAGATG TGTCTGTGGA AGACCAGACA AAGGACACAG GCATCCGGAA AATCCAGGCC 
ATCTCCAGCT TGAGTGACGA CAGTGACCTG GAGCTGGAGG AGCTGCCCCT CTCCGTGTTC 
GAGGGCACTC CCATAAGCCC CACAGGCGCC CTCTCACACA TATTTGCGGT CTCCAAAGAC 
CCCTCAGAGG CAGCCAGGGT GCCTTTCGCA GACATATGTA TGCCAACAGC AACAACAGAC 
TTAGAAACTC AAAGTCAAGA CCCCAGCACA GCATCTTCAC ACCCCCTCAT CCAGGAGCTC 
GGAGAGGATG AGCTGACAGA GGGAGAGTCA AACCAGCCAC TGCCACCCCA AAGCTGTGCA 
AGTGACCCCA CACTTGCCCA GTCTAGTGAA GGTGGAGACA GTCAGCTGCC TGCAGCCACT 
CCACTAGGAG ATGGAGCTGA GAATGAAGCA CAGTCTTCGT TGTACCCAGA GGAGCCCAGC 
ACCCGGATAG GCCTGGAAGA TATTGAATTT GGCTTGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014176.1
CDS	64..1965
Translation	MHPEASEPPVDSAAEPSLEESAGDHGDAGPGVRKEEINETKETCVGPCTTSCQSQQQPSGDNGSDGLFTHSRDDRDDRGPRMTKQFLQKLCKQHKLYVTPALNDTLYLHFKGFDRIENLEEYTGLRCLWLECNGIQRIENLQAQSELRCLFLQVNLLHKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNRLETVADIEHLRECLQLCVLDLSHNSLSDPEILSVLETMPCLRVLNLMGNPVTKHIPNYRRTVTVRLKHLTYLDDRPVFPKDRACAEAWARGGYAAEKEERHQWESREHKKITDSLEALAMIKRRAEERKKARDRGETPLPESEKSIPTSPEAQEKPPKGETQQKMESFVKESFEAKDELFPEKPGEGEELSVVVGNRAVEDADLSGNLAHTQTPVVVTPEEVTSPVEATDGARTEDTEAIALETKEKLFIDDLPDLEDVDGTDVSVEDQTKDTGIRKIQAISSLSDDSDLELEELPLSVFEGTPISPTGALSHIFAVSKDPSEAARVPFADICMPTATTDLETQSQDPSTASSHPLIQELGEDELTEGESNQPLPPQSCASDPTLAQSSEGGDSQLPAATPLGDGAENEAQSSLYPEEPSTRIGLEDIEFGLD

Target ORF information:

RefSeq Version	NM_001014154.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein, axonemal, assembly factor 1 (Dnaaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014154.1

ORF Insert Sequence:

ATGCACCCCG AAGCCTCAGA GCCGCCGGTG GACAGCGCGG CGGAGCCCAG TCTGGAGGAG 
TCCGCAGGCG ATCACGGCGA CGCAGGCCCA GGGGTCCGCA AAGAAGAAAT TAATGAGACC 
AAGGAAACAT GTGTGGGTCC TTGTACAACA TCCTGCCAAA GTCAGCAGCA ACCGAGCGGT 
GACAATGGGT CAGATGGCCT CTTTACCCAC TCGAGAGATG ACAGAGATGA TCGGGGCCCC 
AGGATGACGA AGCAGTTCCT CCAAAAGCTC TGTAAGCAGC ACAAGCTGTA CGTCACCCCG 
GCCCTGAACG ACACCCTGTA TTTGCACTTT AAGGGCTTTG ACCGCATTGA GAACCTGGAG 
GAGTACACGG GCCTGCGCTG CCTCTGGCTG GAGTGCAACG GCATCCAGAG GATCGAGAAT 
TTGCAGGCCC AGAGCGAGCT GCGCTGCCTC TTCCTGCAAG TGAACCTGTT GCACAAGATC 
GAAAACCTGG AGCCGCTCCA GAAGCTGGAC GCCCTCAACC TGAGCAACAA CTACATCAAG 
ACCATTGAGA ACCTCTCCTG TCTGCCGGTC CTTAACACTC TGCAAATGGC CCACAACCGC 
CTGGAGACCG TGGCAGACAT TGAGCACCTC AGGGAGTGTC TGCAACTCTG TGTCCTCGAC 
CTCTCCCACA ACTCGCTGAG TGACCCCGAG ATCCTGAGCG TGCTTGAGAC CATGCCCTGT 
CTGCGTGTGC TGAACCTGAT GGGGAACCCA GTGACCAAAC ACATCCCTAA CTACCGCAGG 
ACGGTCACTG TCCGACTGAA GCACTTGACT TATCTGGACG ACAGGCCGGT TTTCCCCAAG 
GACAGGGCGT GTGCAGAGGC CTGGGCCCGA GGCGGGTATG CTGCCGAGAA GGAAGAGCGT 
CACCAGTGGG AGAGCAGGGA ACACAAGAAG ATAACAGACA GCCTGGAAGC CTTGGCCATG 
ATCAAGCGGC GGGCCGAGGA ACGGAAGAAG GCAAGAGACA GAGGGGAGAC ACCTCTGCCA 
GAGAGTGAAA AGAGTATCCC CACTAGCCCA GAGGCTCAGG AAAAGCCTCC CAAGGGGGAA 
ACCCAGCAGA AGATGGAGTC GTTTGTGAAA GAGAGCTTTG AGGCCAAAGA TGAACTGTTT 
CCAGAGAAGC CAGGAGAAGG AGAGGAGCTG TCTGTGGTGG TGGGGAATAG AGCAGTGGAA 
GATGCAGACC TGTCCGGGAA CCTGGCTCAC ACCCAGACAC CTGTGGTGGT CACCCCTGAA 
GAGGTCACAT CTCCAGTGGA AGCTACTGAT GGGGCCAGAA CGGAAGACAC AGAGGCCATT 
GCCCTGGAGA CCAAGGAGAA GCTTTTCATC GATGACCTGC CTGACTTGGA AGATGTTGAT 
GGTACAGATG TGTCTGTGGA AGACCAGACA AAGGACACAG GCATCCGGAA AATCCAGGCC 
ATCTCCAGCT TGAGTGACGA CAGTGACCTG GAGCTGGAGG AGCTGCCCCT CTCCGTGTTC 
GAGGGCACTC CCATAAGCCC CACAGGCGCC CTCTCACACA TATTTGCGGT CTCCAAAGAC 
CCCTCAGAGG CAGCCAGGGT GCCTTTCGCA GACATATGTA TGCCAACAGC AACAACAGAC 
TTAGAAACTC AAAGTCAAGA CCCCAGCACA GCATCTTCAC ACCCCCTCAT CCAGGAGCTC 
GGAGAGGATG AGCTGACAGA GGGAGAGTCA AACCAGCCAC TGCCACCCCA AAGCTGTGCA 
AGTGACCCCA CACTTGCCCA GTCTAGTGAA GGTGGAGACA GTCAGCTGCC TGCAGCCACT 
CCACTAGGAG ATGGAGCTGA GAATGAAGCA CAGTCTTCGT TGTACCCAGA GGAGCCCAGC 
ACCCGGATAG GCCTGGAAGA TATTGAATTT GGCTTGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnaaf1 ( XM_017601323.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456812.1 Rattus norvegicus dynein, axonemal, assembly factor 1 (Dnaaf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59465
Clone ID Related Accession (Same CDS sequence)	XM_017601323.1
Accession Version	XM_017601323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dynein assembly factor 1, axonemal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCATCACCAA GGGCTGTGCT CCACCCTGGA CCGCAGCAGA TGAGCCACTG 
CAGTCAGATC CAGGGCACAC CAAGCAGCTG GATGAGACCG AAGGCTCACA CACTCACAGT 
AGAACAGAAA TTAATGAGAC CAAGGAAACA TGTGTGGGTC CTTGTACAAC ATCCTGCCAA 
AGTCAGCAGC AACCGAGCGG TGACAATGGG TCAGATGGCC TCTTTACCCA CTCGAGAGAT 
GACAGAGATG ATCGGGGCCC CAGGATGACG AAGCAGTTCC TCCAAAAGCT CTGTAAGCAG 
CACAAGCTGT ACGTCACCCC GGCCCTGAAC GACACCCTGT ATTTGCACTT TAAGGGCTTT 
GACCGCATTG AGAACCTGGA GGAGTACACG GGCCTGCGCT GCCTCTGGCT GGAGTGCAAC 
GGCATCCAGA GGATCGAGAA TTTGCAGGCC CAGAGCGAGC TGCGCTGCCT CTTCCTGCAA 
GTGAACCTGT TGCACAAGAT CGAAAACCTG GAGCCGCTCC AGAAGCTGGA CGCCCTCAAC 
CTGAGCAACA ACTACATCAA GACCATTGAG AACCTCTCCT GTCTGCCGGT CCTTAACACT 
CTGCAAATGG CCCACAACCG CCTGGAGACC GTGGCAGACA TTGAGCACCT CAGGGAGTGT 
CTGCAACTCT GTGTCCTCGA CCTCTCCCAC AACTCGCTGA GTGACCCCGA GATCCTGAGC 
GTGCTTGAGA CCATGCCCTG TCTGCGTGTG CTGAACCTGA TGGGGAACCC AGTGACCAAA 
CACATCCCTA ACTACCGCAG GACGGTCACT GTCCGACTGA AGCACTTGAC TTATCTGGAC 
GACAGGCCGG TTTTCCCCAA GGACAGGGCG TGTGCAGAGG CCTGGGCCCG AGGCGGGTAT 
GCTGCCGAGA AGGAAGAGCG TCACCAGTGG GAGAGCAGGG AACACAAGAA GATAACAGAC 
AGCCTGGAAG CCTTGGCCAT GATCAAGCGG CGGGCCGAGG AACGGAAGAA GGCAAGAGAC 
AGAGGGGAGA CACCTCTGCC AGAGAGTGAA AAGAGTATCC CCACTAGCCC AGAGGCTCAG 
GAAAAGCCTC CCAAGGGGGA AACCCAGCAG AAGATGGAGT CGTTTGTGAA AGAGAGCTTT 
GAGGCCAAAG ATGAACTGTT TCCAGAGAAG CCAGGAGAAG GAGAGGAGCT GTCTGTGGTG 
GTGGGGAATA GAGCAGTGGA AGATGCAGAC CTGTCCGGGA ACCTGGCTCA CACCCAGACA 
CCTGTGGTGG TCACCCCTGA AGAGGTCACA TCTCCAGTGG AAGCTACTGA TGGGGCCAGA 
ACGGAAGACA CAGAGGCCAT TGCCCTGGAG ACCAAGGAGA AGCTTTTCAT CGATGACCTG 
CCTGACTTGG AAGATGTTGA TGGTACAGAT GTGTCTGTGG AAGACCAGAC AAAGGACACA 
GGCATCCGGA AAATCCAGGC CATCTCCAGC TTGAGTGACG ACAGTGACCT GGAGCTGGAG 
GAGCTGCCCC TCTCCGTGTT CGAGGGCACT CCCATAAGCC CCACAGGCGC CCTCTCACAC 
ATATTTGCGG TCTCCAAAGA CCCCTCAGAG GCAGCCAGGG TGCCTTTCGC AGACATATGT 
ATGCCAACAG CAACAACAGA CTTAGAAACT CAAAGTCAAG ACCCCAGCAC AGCATCTTCA 
CACCCCCTCA TCCAGGAGCT CGGAGAGGAT GAGCTGACAG AGGGAGAGTC AAACCAGCCA 
CTGCCACCCC AAAGCTGTGC AAGTGACCCC ACACTTGCCC AGTCTAGTGA AGGTGGAGAC 
AGTCAGCTGC CTGCAGCCAC TCCACTAGGA GATGGAGCTG AGAATGAAGC ACAGTCTTCG 
TTGTACCCAG AGGAGCCCAG CACCCGGATA GGCCTGGAAG ATATTGAATT TGGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456812.1
CDS	663..2585
Translation	MANGITKGCAPPWTAADEPLQSDPGHTKQLDETEGSHTHSRTEINETKETCVGPCTTSCQSQQQPSGDNGSDGLFTHSRDDRDDRGPRMTKQFLQKLCKQHKLYVTPALNDTLYLHFKGFDRIENLEEYTGLRCLWLECNGIQRIENLQAQSELRCLFLQVNLLHKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNRLETVADIEHLRECLQLCVLDLSHNSLSDPEILSVLETMPCLRVLNLMGNPVTKHIPNYRRTVTVRLKHLTYLDDRPVFPKDRACAEAWARGGYAAEKEERHQWESREHKKITDSLEALAMIKRRAEERKKARDRGETPLPESEKSIPTSPEAQEKPPKGETQQKMESFVKESFEAKDELFPEKPGEGEELSVVVGNRAVEDADLSGNLAHTQTPVVVTPEEVTSPVEATDGARTEDTEAIALETKEKLFIDDLPDLEDVDGTDVSVEDQTKDTGIRKIQAISSLSDDSDLELEELPLSVFEGTPISPTGALSHIFAVSKDPSEAARVPFADICMPTATTDLETQSQDPSTASSHPLIQELGEDELTEGESNQPLPPQSCASDPTLAQSSEGGDSQLPAATPLGDGAENEAQSSLYPEEPSTRIGLEDIEFGLD

Target ORF information:

RefSeq Version	XM_017601323.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein, axonemal, assembly factor 1 (Dnaaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601323.1

ORF Insert Sequence:

ATGGCAAATG GCATCACCAA GGGCTGTGCT CCACCCTGGA CCGCAGCAGA TGAGCCACTG 
CAGTCAGATC CAGGGCACAC CAAGCAGCTG GATGAGACCG AAGGCTCACA CACTCACAGT 
AGAACAGAAA TTAATGAGAC CAAGGAAACA TGTGTGGGTC CTTGTACAAC ATCCTGCCAA 
AGTCAGCAGC AACCGAGCGG TGACAATGGG TCAGATGGCC TCTTTACCCA CTCGAGAGAT 
GACAGAGATG ATCGGGGCCC CAGGATGACG AAGCAGTTCC TCCAAAAGCT CTGTAAGCAG 
CACAAGCTGT ACGTCACCCC GGCCCTGAAC GACACCCTGT ATTTGCACTT TAAGGGCTTT 
GACCGCATTG AGAACCTGGA GGAGTACACG GGCCTGCGCT GCCTCTGGCT GGAGTGCAAC 
GGCATCCAGA GGATCGAGAA TTTGCAGGCC CAGAGCGAGC TGCGCTGCCT CTTCCTGCAA 
GTGAACCTGT TGCACAAGAT CGAAAACCTG GAGCCGCTCC AGAAGCTGGA CGCCCTCAAC 
CTGAGCAACA ACTACATCAA GACCATTGAG AACCTCTCCT GTCTGCCGGT CCTTAACACT 
CTGCAAATGG CCCACAACCG CCTGGAGACC GTGGCAGACA TTGAGCACCT CAGGGAGTGT 
CTGCAACTCT GTGTCCTCGA CCTCTCCCAC AACTCGCTGA GTGACCCCGA GATCCTGAGC 
GTGCTTGAGA CCATGCCCTG TCTGCGTGTG CTGAACCTGA TGGGGAACCC AGTGACCAAA 
CACATCCCTA ACTACCGCAG GACGGTCACT GTCCGACTGA AGCACTTGAC TTATCTGGAC 
GACAGGCCGG TTTTCCCCAA GGACAGGGCG TGTGCAGAGG CCTGGGCCCG AGGCGGGTAT 
GCTGCCGAGA AGGAAGAGCG TCACCAGTGG GAGAGCAGGG AACACAAGAA GATAACAGAC 
AGCCTGGAAG CCTTGGCCAT GATCAAGCGG CGGGCCGAGG AACGGAAGAA GGCAAGAGAC 
AGAGGGGAGA CACCTCTGCC AGAGAGTGAA AAGAGTATCC CCACTAGCCC AGAGGCTCAG 
GAAAAGCCTC CCAAGGGGGA AACCCAGCAG AAGATGGAGT CGTTTGTGAA AGAGAGCTTT 
GAGGCCAAAG ATGAACTGTT TCCAGAGAAG CCAGGAGAAG GAGAGGAGCT GTCTGTGGTG 
GTGGGGAATA GAGCAGTGGA AGATGCAGAC CTGTCCGGGA ACCTGGCTCA CACCCAGACA 
CCTGTGGTGG TCACCCCTGA AGAGGTCACA TCTCCAGTGG AAGCTACTGA TGGGGCCAGA 
ACGGAAGACA CAGAGGCCAT TGCCCTGGAG ACCAAGGAGA AGCTTTTCAT CGATGACCTG 
CCTGACTTGG AAGATGTTGA TGGTACAGAT GTGTCTGTGG AAGACCAGAC AAAGGACACA 
GGCATCCGGA AAATCCAGGC CATCTCCAGC TTGAGTGACG ACAGTGACCT GGAGCTGGAG 
GAGCTGCCCC TCTCCGTGTT CGAGGGCACT CCCATAAGCC CCACAGGCGC CCTCTCACAC 
ATATTTGCGG TCTCCAAAGA CCCCTCAGAG GCAGCCAGGG TGCCTTTCGC AGACATATGT 
ATGCCAACAG CAACAACAGA CTTAGAAACT CAAAGTCAAG ACCCCAGCAC AGCATCTTCA 
CACCCCCTCA TCCAGGAGCT CGGAGAGGAT GAGCTGACAG AGGGAGAGTC AAACCAGCCA 
CTGCCACCCC AAAGCTGTGC AAGTGACCCC ACACTTGCCC AGTCTAGTGA AGGTGGAGAC 
AGTCAGCTGC CTGCAGCCAC TCCACTAGGA GATGGAGCTG AGAATGAAGC ACAGTCTTCG 
TTGTACCCAG AGGAGCCCAG CACCCGGATA GGCCTGGAAG ATATTGAATT TGGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletions and point mutations of LRRC50 cause primary ciliary dyskinesia due to dynein arm defects.
American journal of human genetics85(6)883-9(2009 Dec)
Loges NT,Olbrich H,Becker-Heck A,H?ffner K,Heer A,Reinhard C,Schmidts M,Kispert A,Zariwala MA,Leigh MW,Knowles MR,Zentgraf H,Seithe H,N?rnberg G,N?rnberg P,Reinhardt R,Omran H

Loss-of-function mutations in the human ortholog of Chlamydomonas reinhardtii ODA7 disrupt dynein arm assembly and cause primary ciliary dyskinesia.
American journal of human genetics85(6)890-6(2009 Dec)
Duquesnoy P,Escudier E,Vincensini L,Freshour J,Bridoux AM,Coste A,Deschildre A,de Blic J,Legendre M,Montantin G,Tenreiro H,Vojtek AM,Loussert C,Cl?ment A,Escalier D,Bastin P,Mitchell DR,Amselem S

LRRC50, a conserved ciliary protein implicated in polycystic kidney disease.
Journal of the American Society of Nephrology : JASN19(6)1128-38(2008 Jun)
van Rooijen E,Giles RH,Voest EE,van Rooijen C,Schulte-Merker S,van Eeden FJ

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Dnaaf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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