Cdkal1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdkal1
Entrez Gene ID	361243
Full Name	CDK5 regulatory subunit associated protein 1-like 1
General protein information	Preferred Names CDK5 regulatory subunit associated protein 1-like 1 Names CDK5 regulatory subunit-associated protein 1-like 1 threonylcarbamoyladenosine tRNA methylthiotransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006222382.2	XP_006222444.1	threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2
XM_008773955.2	XP_008772177.1	threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2
XM_008771610.2	XP_008769832.2	threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1
XM_017587765.1	XP_017443254.1	threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2
NM_001108413.1	NP_001101883.1	CDK5 regulatory subunit-associated protein 1-like 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

CDK5 regulatory subunit-associated protein 1-like 1 (CDKAL1) is a tail-anchored protein in the endoplasmic reticulum (ER) of insulinoma cells.
Brambillasca S, Altkrueger A, Colombo SF, Friederich A, Eickelmann P, Mark M, Borgese N, Solimena M
The Journal of biological chemistry287(50)41808-19(2012 Dec)

Deficit of tRNA(Lys) modification by Cdkal1 causes the development of type 2 diabetes in mice.
Wei FY, Suzuki T, Watanabe S, Kimura S, Kaitsuka T, Fujimura A, Matsui H, Atta M, Michiue H, Fontecave M, Yamagata K, Suzuki T, Tomizawa K
The Journal of clinical investigation121(9)3598-608(2011 Sep)

Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosis.
Ohara-Imaizumi M, Yoshida M, Aoyagi K, Saito T, Okamura T, Takenaka H, Akimoto Y, Nakamichi Y, Takanashi-Yanobu R, Nishiwaki C, Kawakami H, Kato N, Hisanaga S, Kakei M, Nagamatsu S
PloS one5(12)e15553(2010 Dec)

Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
Arragain S, Handelman SK, Forouhar F, Wei FY, Tomizawa K, Hunt JF, Douki T, Fontecave M, Mulliez E, Atta M
The Journal of biological chemistry285(37)28425-33(2010 Sep)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CDK5 regulatory subunit-associated protein 1-like 1 (CDKAL1) is a tail-anchored protein in the endoplasmic reticulum (ER) of insulinoma cells.
Title: CDK5 regulatory subunit-associated protein 1-like 1 (CDKAL1) is a tail-anchored protein in the endoplasmic reticulum (ER) of insulinoma cells.

The following Cdkal1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdkal1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa20654	XM_006222382.2 Latest version!	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa20654	XM_008771610.2 Latest version!	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa20654	XM_008773955.2 Latest version!	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa20654	XM_017587765.1 Latest version!	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa63322	NM_001108413.1 Latest version!	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdkal1 ( XM_006222382.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006222444.1 Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa20654
Clone ID Related Accession (Same CDS sequence)	XM_006222382.2 , XM_008773955.2 , XM_008771610.2 , XM_017587765.1
Accession Version	XM_006222382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000085.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006222382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGAAAATGC ATCAGATGCA 
GATCTGTGGC TCCTGAACAG CTGCACTGTG AAGAACCCAG CTGAAGACCA CTTCAGAAAC 
TCAATCAAAA AAGCTCACGA GGAGAATAAG AAAGTTGTCC TCGCTGGCTG TGTTCCCCAA 
GCCCAGCCTC GTCAGGATTA CCTCAAAGGA CTGAGCATCA TTGGGGTTCA GCAGATAGAT 
CGTGTGGTAG AAGTTGTGGA GGAAACAATA AAAGGTCACT CCGTGAGGCT CCTGGGCCAG 
AAGAAAGAAA GTGGGAAGCG GTTGGGTGGA GCGAGACTGG ATTTGCCAAA GATCAGGAAG 
AATCCGCTGA TAGAAATCAT ATCCATCAAC ACGGGGTGTC TCAATGCTTG TACCTACTGC 
AAAACTAAAC ATGCCAGAGG AAACCTGGCC AGCTATCCCA TTGATGAACT TGTAGAGAGA 
GCCAAGCAGT CCTTTCAAGA GGGTGTTTGT GAGATATGGC TGACCAGCGA GGACACCGGG 
GCCTATGGCA GAGACATTGG CACAGATCTC CCCACACTCC TGTGGAAGCT GGTGGAAGTG 
ATTCCTGAGG GAGCCATGCT GAGGCTTGGC ATGACAAATC CACCGTATAT TTTAGAGCAT 
CTGGAGGAAA TGGCAAAAAT CCTCAACCAC CCCAGAGTCT ACGCTTTTCT CCACATCCCG 
GTGCAGTCTG CCTCTGACAG TGTGCTCATG GACATGAAGA GAGAGTACTG CGTGGCCGAC 
TTCAAAAGAG TTGTGGATTT TCTCAAGGAG AAGGTTCCTG GAATAACGAT TGCTACGGAT 
ATTATCTGTG GTTTTCCTGG AGAAACCGAT CAGGATTTTC AAGAAACCGT GAAACTTGTT 
GAAGAGTACA AATTTCCAAG CCTCTTCATC AACCAGTTCT ATCCAAGACC CGGAACCCCC 
GCTGCAAAAG CCAAACAAGT CCCAGCACAC GTGAAAAAAC AAAGAACAAA AGATCTATCT 
CGGGTGTTTC ATTCCTACAA CCCATATGAT CACAAGATTG GCGAAAGACA ACAAGTGTTA 
GTCACAGAAG AGTCTTTTGA CTCCAAGTTT TATGTTGCAC ATAACCGATT CTATGAGCAG 
GTCTTAGTGC CAAAGAACCC TGCATTCATG GGAAAAATGG TTGAGGTCGA CATTTATGAA 
TCAGGAAAAC ACTTCTTGAA AGGACAGCCA GTATCAGAAA CCAGAGTCTA TACACCCTCC 
ATCTGCAAGC CGCTGGCCAA GGGAGAAGTC TCAGGTTTGA CAACGGAATT CAGAAATAGA 
CTTGGGGATC GTCTGAATTC AACTTCGGGC ACCTGTCCTG CACCTCCACA GGGCTCGGCC 
TGTTCCAGAA TGGCGCTGCA GACGTCCCAG TATGACTGTG CACTGAAGGT GGCTGCAGGC 
CTGGCTCTCA TCGCTCTCCT TCTGCATTTC TGGCCTGAGT CTATGTTTAC AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006222444.1
CDS	154..1890
Translation	MPSASCDVLLDDIEDIISQEDSKPQDRHFSRKHVFPKVRRRNTQKYLQEEPSPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAHEENKKVVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKESGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVERAKQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKAKQVPAHVKKQRTKDLSRVFHSYNPYDHKIGERQQVLVTEESFDSKFYVAHNRFYEQVLVPKNPAFMGKMVEVDIYESGKHFLKGQPVSETRVYTPSICKPLAKGEVSGLTTEFRNRLGDRLNSTSGTCPAPPQGSACSRMALQTSQYDCALKVAAGLALIALLLHFWPESMFTM

Target ORF information:

RefSeq Version	XM_006222382.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006222382.2

ORF Insert Sequence:

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGAAAATGC ATCAGATGCA 
GATCTGTGGC TCCTGAACAG CTGCACTGTG AAGAACCCAG CTGAAGACCA CTTCAGAAAC 
TCAATCAAAA AAGCTCACGA GGAGAATAAG AAAGTTGTCC TCGCTGGCTG TGTTCCCCAA 
GCCCAGCCTC GTCAGGATTA CCTCAAAGGA CTGAGCATCA TTGGGGTTCA GCAGATAGAT 
CGTGTGGTAG AAGTTGTGGA GGAAACAATA AAAGGTCACT CCGTGAGGCT CCTGGGCCAG 
AAGAAAGAAA GTGGGAAGCG GTTGGGTGGA GCGAGACTGG ATTTGCCAAA GATCAGGAAG 
AATCCGCTGA TAGAAATCAT ATCCATCAAC ACGGGGTGTC TCAATGCTTG TACCTACTGC 
AAAACTAAAC ATGCCAGAGG AAACCTGGCC AGCTATCCCA TTGATGAACT TGTAGAGAGA 
GCCAAGCAGT CCTTTCAAGA GGGTGTTTGT GAGATATGGC TGACCAGCGA GGACACCGGG 
GCCTATGGCA GAGACATTGG CACAGATCTC CCCACACTCC TGTGGAAGCT GGTGGAAGTG 
ATTCCTGAGG GAGCCATGCT GAGGCTTGGC ATGACAAATC CACCGTATAT TTTAGAGCAT 
CTGGAGGAAA TGGCAAAAAT CCTCAACCAC CCCAGAGTCT ACGCTTTTCT CCACATCCCG 
GTGCAGTCTG CCTCTGACAG TGTGCTCATG GACATGAAGA GAGAGTACTG CGTGGCCGAC 
TTCAAAAGAG TTGTGGATTT TCTCAAGGAG AAGGTTCCTG GAATAACGAT TGCTACGGAT 
ATTATCTGTG GTTTTCCTGG AGAAACCGAT CAGGATTTTC AAGAAACCGT GAAACTTGTT 
GAAGAGTACA AATTTCCAAG CCTCTTCATC AACCAGTTCT ATCCAAGACC CGGAACCCCC 
GCTGCAAAAG CCAAACAAGT CCCAGCACAC GTGAAAAAAC AAAGAACAAA AGATCTATCT 
CGGGTGTTTC ATTCCTACAA CCCATATGAT CACAAGATTG GCGAAAGACA ACAAGTGTTA 
GTCACAGAAG AGTCTTTTGA CTCCAAGTTT TATGTTGCAC ATAACCGATT CTATGAGCAG 
GTCTTAGTGC CAAAGAACCC TGCATTCATG GGAAAAATGG TTGAGGTCGA CATTTATGAA 
TCAGGAAAAC ACTTCTTGAA AGGACAGCCA GTATCAGAAA CCAGAGTCTA TACACCCTCC 
ATCTGCAAGC CGCTGGCCAA GGGAGAAGTC TCAGGTTTGA CAACGGAATT CAGAAATAGA 
CTTGGGGATC GTCTGAATTC AACTTCGGGC ACCTGTCCTG CACCTCCACA GGGCTCGGCC 
TGTTCCAGAA TGGCGCTGCA GACGTCCCAG TATGACTGTG CACTGAAGGT GGCTGCAGGC 
CTGGCTCTCA TCGCTCTCCT TCTGCATTTC TGGCCTGAGT CTATGTTTAC AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdkal1 ( XM_008773955.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008772177.1 Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa20654
Clone ID Related Accession (Same CDS sequence)	XM_006222382.2 , XM_008773955.2 , XM_008771610.2 , XM_017587765.1
Accession Version	XM_008773955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGAAAATGC ATCAGATGCA 
GATCTGTGGC TCCTGAACAG CTGCACTGTG AAGAACCCAG CTGAAGACCA CTTCAGAAAC 
TCAATCAAAA AAGCTCACGA GGAGAATAAG AAAGTTGTCC TCGCTGGCTG TGTTCCCCAA 
GCCCAGCCTC GTCAGGATTA CCTCAAAGGA CTGAGCATCA TTGGGGTTCA GCAGATAGAT 
CGTGTGGTAG AAGTTGTGGA GGAAACAATA AAAGGTCACT CCGTGAGGCT CCTGGGCCAG 
AAGAAAGAAA GTGGGAAGCG GTTGGGTGGA GCGAGACTGG ATTTGCCAAA GATCAGGAAG 
AATCCGCTGA TAGAAATCAT ATCCATCAAC ACGGGGTGTC TCAATGCTTG TACCTACTGC 
AAAACTAAAC ATGCCAGAGG AAACCTGGCC AGCTATCCCA TTGATGAACT TGTAGAGAGA 
GCCAAGCAGT CCTTTCAAGA GGGTGTTTGT GAGATATGGC TGACCAGCGA GGACACCGGG 
GCCTATGGCA GAGACATTGG CACAGATCTC CCCACACTCC TGTGGAAGCT GGTGGAAGTG 
ATTCCTGAGG GAGCCATGCT GAGGCTTGGC ATGACAAATC CACCGTATAT TTTAGAGCAT 
CTGGAGGAAA TGGCAAAAAT CCTCAACCAC CCCAGAGTCT ACGCTTTTCT CCACATCCCG 
GTGCAGTCTG CCTCTGACAG TGTGCTCATG GACATGAAGA GAGAGTACTG CGTGGCCGAC 
TTCAAAAGAG TTGTGGATTT TCTCAAGGAG AAGGTTCCTG GAATAACGAT TGCTACGGAT 
ATTATCTGTG GTTTTCCTGG AGAAACCGAT CAGGATTTTC AAGAAACCGT GAAACTTGTT 
GAAGAGTACA AATTTCCAAG CCTCTTCATC AACCAGTTCT ATCCAAGACC CGGAACCCCC 
GCTGCAAAAG CCAAACAAGT CCCAGCACAC GTGAAAAAAC AAAGAACAAA AGATCTATCT 
CGGGTGTTTC ATTCCTACAA CCCATATGAT CACAAGATTG GCGAAAGACA ACAAGTGTTA 
GTCACAGAAG AGTCTTTTGA CTCCAAGTTT TATGTTGCAC ATAACCGATT CTATGAGCAG 
GTCTTAGTGC CAAAGAACCC TGCATTCATG GGAAAAATGG TTGAGGTCGA CATTTATGAA 
TCAGGAAAAC ACTTCTTGAA AGGACAGCCA GTATCAGAAA CCAGAGTCTA TACACCCTCC 
ATCTGCAAGC CGCTGGCCAA GGGAGAAGTC TCAGGTTTGA CAACGGAATT CAGAAATAGA 
CTTGGGGATC GTCTGAATTC AACTTCGGGC ACCTGTCCTG CACCTCCACA GGGCTCGGCC 
TGTTCCAGAA TGGCGCTGCA GACGTCCCAG TATGACTGTG CACTGAAGGT GGCTGCAGGC 
CTGGCTCTCA TCGCTCTCCT TCTGCATTTC TGGCCTGAGT CTATGTTTAC AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008772177.1
CDS	1807..3543
Translation	MPSASCDVLLDDIEDIISQEDSKPQDRHFSRKHVFPKVRRRNTQKYLQEEPSPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAHEENKKVVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKESGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVERAKQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKAKQVPAHVKKQRTKDLSRVFHSYNPYDHKIGERQQVLVTEESFDSKFYVAHNRFYEQVLVPKNPAFMGKMVEVDIYESGKHFLKGQPVSETRVYTPSICKPLAKGEVSGLTTEFRNRLGDRLNSTSGTCPAPPQGSACSRMALQTSQYDCALKVAAGLALIALLLHFWPESMFTM

Target ORF information:

RefSeq Version	XM_008773955.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773955.2

ORF Insert Sequence:

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGAAAATGC ATCAGATGCA 
GATCTGTGGC TCCTGAACAG CTGCACTGTG AAGAACCCAG CTGAAGACCA CTTCAGAAAC 
TCAATCAAAA AAGCTCACGA GGAGAATAAG AAAGTTGTCC TCGCTGGCTG TGTTCCCCAA 
GCCCAGCCTC GTCAGGATTA CCTCAAAGGA CTGAGCATCA TTGGGGTTCA GCAGATAGAT 
CGTGTGGTAG AAGTTGTGGA GGAAACAATA AAAGGTCACT CCGTGAGGCT CCTGGGCCAG 
AAGAAAGAAA GTGGGAAGCG GTTGGGTGGA GCGAGACTGG ATTTGCCAAA GATCAGGAAG 
AATCCGCTGA TAGAAATCAT ATCCATCAAC ACGGGGTGTC TCAATGCTTG TACCTACTGC 
AAAACTAAAC ATGCCAGAGG AAACCTGGCC AGCTATCCCA TTGATGAACT TGTAGAGAGA 
GCCAAGCAGT CCTTTCAAGA GGGTGTTTGT GAGATATGGC TGACCAGCGA GGACACCGGG 
GCCTATGGCA GAGACATTGG CACAGATCTC CCCACACTCC TGTGGAAGCT GGTGGAAGTG 
ATTCCTGAGG GAGCCATGCT GAGGCTTGGC ATGACAAATC CACCGTATAT TTTAGAGCAT 
CTGGAGGAAA TGGCAAAAAT CCTCAACCAC CCCAGAGTCT ACGCTTTTCT CCACATCCCG 
GTGCAGTCTG CCTCTGACAG TGTGCTCATG GACATGAAGA GAGAGTACTG CGTGGCCGAC 
TTCAAAAGAG TTGTGGATTT TCTCAAGGAG AAGGTTCCTG GAATAACGAT TGCTACGGAT 
ATTATCTGTG GTTTTCCTGG AGAAACCGAT CAGGATTTTC AAGAAACCGT GAAACTTGTT 
GAAGAGTACA AATTTCCAAG CCTCTTCATC AACCAGTTCT ATCCAAGACC CGGAACCCCC 
GCTGCAAAAG CCAAACAAGT CCCAGCACAC GTGAAAAAAC AAAGAACAAA AGATCTATCT 
CGGGTGTTTC ATTCCTACAA CCCATATGAT CACAAGATTG GCGAAAGACA ACAAGTGTTA 
GTCACAGAAG AGTCTTTTGA CTCCAAGTTT TATGTTGCAC ATAACCGATT CTATGAGCAG 
GTCTTAGTGC CAAAGAACCC TGCATTCATG GGAAAAATGG TTGAGGTCGA CATTTATGAA 
TCAGGAAAAC ACTTCTTGAA AGGACAGCCA GTATCAGAAA CCAGAGTCTA TACACCCTCC 
ATCTGCAAGC CGCTGGCCAA GGGAGAAGTC TCAGGTTTGA CAACGGAATT CAGAAATAGA 
CTTGGGGATC GTCTGAATTC AACTTCGGGC ACCTGTCCTG CACCTCCACA GGGCTCGGCC 
TGTTCCAGAA TGGCGCTGCA GACGTCCCAG TATGACTGTG CACTGAAGGT GGCTGCAGGC 
CTGGCTCTCA TCGCTCTCCT TCTGCATTTC TGGCCTGAGT CTATGTTTAC AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdkal1 ( XM_008771610.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769832.2 Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa20654
Clone ID Related Accession (Same CDS sequence)	XM_006222382.2 , XM_008773955.2 , XM_008771610.2 , XM_017587765.1
Accession Version	XM_008771610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) and transcript sequence (JQ935002.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 356 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGAAAATGC ATCAGATGCA 
GATCTGTGGC TCCTGAACAG CTGCACTGTG AAGAACCCAG CTGAAGACCA CTTCAGAAAC 
TCAATCAAAA AAGCTCACGA GGAGAATAAG AAAGTTGTCC TCGCTGGCTG TGTTCCCCAA 
GCCCAGCCTC GTCAGGATTA CCTCAAAGGA CTGAGCATCA TTGGGGTTCA GCAGATAGAT 
CGTGTGGTAG AAGTTGTGGA GGAAACAATA AAAGGTCACT CCGTGAGGCT CCTGGGCCAG 
AAGAAAGAAA GTGGGAAGCG GTTGGGTGGA GCGAGACTGG ATTTGCCAAA GATCAGGAAG 
AATCCGCTGA TAGAAATCAT ATCCATCAAC ACGGGGTGTC TCAATGCTTG TACCTACTGC 
AAAACTAAAC ATGCCAGAGG AAACCTGGCC AGCTATCCCA TTGATGAACT TGTAGAGAGA 
GCCAAGCAGT CCTTTCAAGA GGGTGTTTGT GAGATATGGC TGACCAGCGA GGACACCGGG 
GCCTATGGCA GAGACATTGG CACAGATCTC CCCACACTCC TGTGGAAGCT GGTGGAAGTG 
ATTCCTGAGG GAGCCATGCT GAGGCTTGGC ATGACAAATC CACCGTATAT TTTAGAGCAT 
CTGGAGGAAA TGGCAAAAAT CCTCAACCAC CCCAGAGTCT ACGCTTTTCT CCACATCCCG 
GTGCAGTCTG CCTCTGACAG TGTGCTCATG GACATGAAGA GAGAGTACTG CGTGGCCGAC 
TTCAAAAGAG TTGTGGATTT TCTCAAGGAG AAGGTTCCTG GAATAACGAT TGCTACGGAT 
ATTATCTGTG GTTTTCCTGG AGAAACCGAT CAGGATTTTC AAGAAACCGT GAAACTTGTT 
GAAGAGTACA AATTTCCAAG CCTCTTCATC AACCAGTTCT ATCCAAGACC CGGAACCCCC 
GCTGCAAAAG CCAAACAAGT CCCAGCACAC GTGAAAAAAC AAAGAACAAA AGATCTATCT 
CGGGTGTTTC ATTCCTACAA CCCATATGAT CACAAGATTG GCGAAAGACA ACAAGTGTTA 
GTCACAGAAG AGTCTTTTGA CTCCAAGTTT TATGTTGCAC ATAACCGATT CTATGAGCAG 
GTCTTAGTGC CAAAGAACCC TGCATTCATG GGAAAAATGG TTGAGGTCGA CATTTATGAA 
TCAGGAAAAC ACTTCTTGAA AGGACAGCCA GTATCAGAAA CCAGAGTCTA TACACCCTCC 
ATCTGCAAGC CGCTGGCCAA GGGAGAAGTC TCAGGTTTGA CAACGGAATT CAGAAATAGA 
CTTGGGGATC GTCTGAATTC AACTTCGGGC ACCTGTCCTG CACCTCCACA GGGCTCGGCC 
TGTTCCAGAA TGGCGCTGCA GACGTCCCAG TATGACTGTG CACTGAAGGT GGCTGCAGGC 
CTGGCTCTCA TCGCTCTCCT TCTGCATTTC TGGCCTGAGT CTATGTTTAC AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769832.2
CDS	154..1890
Translation	MPSASCDVLLDDIEDIISQEDSKPQDRHFSRKHVFPKVRRRNTQKYLQEEPSPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAHEENKKVVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKESGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVERAKQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKAKQVPAHVKKQRTKDLSRVFHSYNPYDHKIGERQQVLVTEESFDSKFYVAHNRFYEQVLVPKNPAFMGKMVEVDIYESGKHFLKGQPVSETRVYTPSICKPLAKGEVSGLTTEFRNRLGDRLNSTSGTCPAPPQGSACSRMALQTSQYDCALKVAAGLALIALLLHFWPESMFTM

Target ORF information:

RefSeq Version	XM_008771610.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771610.2

ORF Insert Sequence:

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGAAAATGC ATCAGATGCA 
GATCTGTGGC TCCTGAACAG CTGCACTGTG AAGAACCCAG CTGAAGACCA CTTCAGAAAC 
TCAATCAAAA AAGCTCACGA GGAGAATAAG AAAGTTGTCC TCGCTGGCTG TGTTCCCCAA 
GCCCAGCCTC GTCAGGATTA CCTCAAAGGA CTGAGCATCA TTGGGGTTCA GCAGATAGAT 
CGTGTGGTAG AAGTTGTGGA GGAAACAATA AAAGGTCACT CCGTGAGGCT CCTGGGCCAG 
AAGAAAGAAA GTGGGAAGCG GTTGGGTGGA GCGAGACTGG ATTTGCCAAA GATCAGGAAG 
AATCCGCTGA TAGAAATCAT ATCCATCAAC ACGGGGTGTC TCAATGCTTG TACCTACTGC 
AAAACTAAAC ATGCCAGAGG AAACCTGGCC AGCTATCCCA TTGATGAACT TGTAGAGAGA 
GCCAAGCAGT CCTTTCAAGA GGGTGTTTGT GAGATATGGC TGACCAGCGA GGACACCGGG 
GCCTATGGCA GAGACATTGG CACAGATCTC CCCACACTCC TGTGGAAGCT GGTGGAAGTG 
ATTCCTGAGG GAGCCATGCT GAGGCTTGGC ATGACAAATC CACCGTATAT TTTAGAGCAT 
CTGGAGGAAA TGGCAAAAAT CCTCAACCAC CCCAGAGTCT ACGCTTTTCT CCACATCCCG 
GTGCAGTCTG CCTCTGACAG TGTGCTCATG GACATGAAGA GAGAGTACTG CGTGGCCGAC 
TTCAAAAGAG TTGTGGATTT TCTCAAGGAG AAGGTTCCTG GAATAACGAT TGCTACGGAT 
ATTATCTGTG GTTTTCCTGG AGAAACCGAT CAGGATTTTC AAGAAACCGT GAAACTTGTT 
GAAGAGTACA AATTTCCAAG CCTCTTCATC AACCAGTTCT ATCCAAGACC CGGAACCCCC 
GCTGCAAAAG CCAAACAAGT CCCAGCACAC GTGAAAAAAC AAAGAACAAA AGATCTATCT 
CGGGTGTTTC ATTCCTACAA CCCATATGAT CACAAGATTG GCGAAAGACA ACAAGTGTTA 
GTCACAGAAG AGTCTTTTGA CTCCAAGTTT TATGTTGCAC ATAACCGATT CTATGAGCAG 
GTCTTAGTGC CAAAGAACCC TGCATTCATG GGAAAAATGG TTGAGGTCGA CATTTATGAA 
TCAGGAAAAC ACTTCTTGAA AGGACAGCCA GTATCAGAAA CCAGAGTCTA TACACCCTCC 
ATCTGCAAGC CGCTGGCCAA GGGAGAAGTC TCAGGTTTGA CAACGGAATT CAGAAATAGA 
CTTGGGGATC GTCTGAATTC AACTTCGGGC ACCTGTCCTG CACCTCCACA GGGCTCGGCC 
TGTTCCAGAA TGGCGCTGCA GACGTCCCAG TATGACTGTG CACTGAAGGT GGCTGCAGGC 
CTGGCTCTCA TCGCTCTCCT TCTGCATTTC TGGCCTGAGT CTATGTTTAC AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdkal1 ( XM_017587765.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017443254.1 Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X3, mRNA.

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CloneID	ORa20654
Clone ID Related Accession (Same CDS sequence)	XM_006222382.2 , XM_008773955.2 , XM_008771610.2 , XM_017587765.1
Accession Version	XM_017587765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGAAAATGC ATCAGATGCA 
GATCTGTGGC TCCTGAACAG CTGCACTGTG AAGAACCCAG CTGAAGACCA CTTCAGAAAC 
TCAATCAAAA AAGCTCACGA GGAGAATAAG AAAGTTGTCC TCGCTGGCTG TGTTCCCCAA 
GCCCAGCCTC GTCAGGATTA CCTCAAAGGA CTGAGCATCA TTGGGGTTCA GCAGATAGAT 
CGTGTGGTAG AAGTTGTGGA GGAAACAATA AAAGGTCACT CCGTGAGGCT CCTGGGCCAG 
AAGAAAGAAA GTGGGAAGCG GTTGGGTGGA GCGAGACTGG ATTTGCCAAA GATCAGGAAG 
AATCCGCTGA TAGAAATCAT ATCCATCAAC ACGGGGTGTC TCAATGCTTG TACCTACTGC 
AAAACTAAAC ATGCCAGAGG AAACCTGGCC AGCTATCCCA TTGATGAACT TGTAGAGAGA 
GCCAAGCAGT CCTTTCAAGA GGGTGTTTGT GAGATATGGC TGACCAGCGA GGACACCGGG 
GCCTATGGCA GAGACATTGG CACAGATCTC CCCACACTCC TGTGGAAGCT GGTGGAAGTG 
ATTCCTGAGG GAGCCATGCT GAGGCTTGGC ATGACAAATC CACCGTATAT TTTAGAGCAT 
CTGGAGGAAA TGGCAAAAAT CCTCAACCAC CCCAGAGTCT ACGCTTTTCT CCACATCCCG 
GTGCAGTCTG CCTCTGACAG TGTGCTCATG GACATGAAGA GAGAGTACTG CGTGGCCGAC 
TTCAAAAGAG TTGTGGATTT TCTCAAGGAG AAGGTTCCTG GAATAACGAT TGCTACGGAT 
ATTATCTGTG GTTTTCCTGG AGAAACCGAT CAGGATTTTC AAGAAACCGT GAAACTTGTT 
GAAGAGTACA AATTTCCAAG CCTCTTCATC AACCAGTTCT ATCCAAGACC CGGAACCCCC 
GCTGCAAAAG CCAAACAAGT CCCAGCACAC GTGAAAAAAC AAAGAACAAA AGATCTATCT 
CGGGTGTTTC ATTCCTACAA CCCATATGAT CACAAGATTG GCGAAAGACA ACAAGTGTTA 
GTCACAGAAG AGTCTTTTGA CTCCAAGTTT TATGTTGCAC ATAACCGATT CTATGAGCAG 
GTCTTAGTGC CAAAGAACCC TGCATTCATG GGAAAAATGG TTGAGGTCGA CATTTATGAA 
TCAGGAAAAC ACTTCTTGAA AGGACAGCCA GTATCAGAAA CCAGAGTCTA TACACCCTCC 
ATCTGCAAGC CGCTGGCCAA GGGAGAAGTC TCAGGTTTGA CAACGGAATT CAGAAATAGA 
CTTGGGGATC GTCTGAATTC AACTTCGGGC ACCTGTCCTG CACCTCCACA GGGCTCGGCC 
TGTTCCAGAA TGGCGCTGCA GACGTCCCAG TATGACTGTG CACTGAAGGT GGCTGCAGGC 
CTGGCTCTCA TCGCTCTCCT TCTGCATTTC TGGCCTGAGT CTATGTTTAC AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017443254.1
CDS	382..2118
Translation	MPSASCDVLLDDIEDIISQEDSKPQDRHFSRKHVFPKVRRRNTQKYLQEEPSPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAHEENKKVVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKESGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVERAKQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKAKQVPAHVKKQRTKDLSRVFHSYNPYDHKIGERQQVLVTEESFDSKFYVAHNRFYEQVLVPKNPAFMGKMVEVDIYESGKHFLKGQPVSETRVYTPSICKPLAKGEVSGLTTEFRNRLGDRLNSTSGTCPAPPQGSACSRMALQTSQYDCALKVAAGLALIALLLHFWPESMFTM

Target ORF information:

RefSeq Version	XM_017587765.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017587765.1

ORF Insert Sequence:

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGAAAATGC ATCAGATGCA 
GATCTGTGGC TCCTGAACAG CTGCACTGTG AAGAACCCAG CTGAAGACCA CTTCAGAAAC 
TCAATCAAAA AAGCTCACGA GGAGAATAAG AAAGTTGTCC TCGCTGGCTG TGTTCCCCAA 
GCCCAGCCTC GTCAGGATTA CCTCAAAGGA CTGAGCATCA TTGGGGTTCA GCAGATAGAT 
CGTGTGGTAG AAGTTGTGGA GGAAACAATA AAAGGTCACT CCGTGAGGCT CCTGGGCCAG 
AAGAAAGAAA GTGGGAAGCG GTTGGGTGGA GCGAGACTGG ATTTGCCAAA GATCAGGAAG 
AATCCGCTGA TAGAAATCAT ATCCATCAAC ACGGGGTGTC TCAATGCTTG TACCTACTGC 
AAAACTAAAC ATGCCAGAGG AAACCTGGCC AGCTATCCCA TTGATGAACT TGTAGAGAGA 
GCCAAGCAGT CCTTTCAAGA GGGTGTTTGT GAGATATGGC TGACCAGCGA GGACACCGGG 
GCCTATGGCA GAGACATTGG CACAGATCTC CCCACACTCC TGTGGAAGCT GGTGGAAGTG 
ATTCCTGAGG GAGCCATGCT GAGGCTTGGC ATGACAAATC CACCGTATAT TTTAGAGCAT 
CTGGAGGAAA TGGCAAAAAT CCTCAACCAC CCCAGAGTCT ACGCTTTTCT CCACATCCCG 
GTGCAGTCTG CCTCTGACAG TGTGCTCATG GACATGAAGA GAGAGTACTG CGTGGCCGAC 
TTCAAAAGAG TTGTGGATTT TCTCAAGGAG AAGGTTCCTG GAATAACGAT TGCTACGGAT 
ATTATCTGTG GTTTTCCTGG AGAAACCGAT CAGGATTTTC AAGAAACCGT GAAACTTGTT 
GAAGAGTACA AATTTCCAAG CCTCTTCATC AACCAGTTCT ATCCAAGACC CGGAACCCCC 
GCTGCAAAAG CCAAACAAGT CCCAGCACAC GTGAAAAAAC AAAGAACAAA AGATCTATCT 
CGGGTGTTTC ATTCCTACAA CCCATATGAT CACAAGATTG GCGAAAGACA ACAAGTGTTA 
GTCACAGAAG AGTCTTTTGA CTCCAAGTTT TATGTTGCAC ATAACCGATT CTATGAGCAG 
GTCTTAGTGC CAAAGAACCC TGCATTCATG GGAAAAATGG TTGAGGTCGA CATTTATGAA 
TCAGGAAAAC ACTTCTTGAA AGGACAGCCA GTATCAGAAA CCAGAGTCTA TACACCCTCC 
ATCTGCAAGC CGCTGGCCAA GGGAGAAGTC TCAGGTTTGA CAACGGAATT CAGAAATAGA 
CTTGGGGATC GTCTGAATTC AACTTCGGGC ACCTGTCCTG CACCTCCACA GGGCTCGGCC 
TGTTCCAGAA TGGCGCTGCA GACGTCCCAG TATGACTGTG CACTGAAGGT GGCTGCAGGC 
CTGGCTCTCA TCGCTCTCCT TCTGCATTTC TGGCCTGAGT CTATGTTTAC AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdkal1 ( NM_001108413.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101883.1 Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), mRNA.

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CloneID	ORa63322
Clone ID Related Accession (Same CDS sequence)	NM_001108413.1
Accession Version	NM_001108413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-28
Organism	Rattus norvegicus(Norway rat)
Product	CDK5 regulatory subunit-associated protein 1-like 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473977.1. On or before Oct 4, 2007 this sequence version replaced XM_341524.3, XM_001061875.1. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGCTCAGTG TGGAAAAAGC 
CAGGCGTGGG CCTCATGGAA ATCATTCACC GTTGTCTTCT GTTTCTCAGG GGCTCACTGT 
CCTTCACCCC GTCAGCTTCA GGGTCTGAAA GCACTCTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101883.1
CDS	146..547
Translation	MPSASCDVLLDDIEDIISQEDSKPQDRHFSRKHVFPKVRRRNTQKYLQEEPSPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITAQCGKSQAWASWKSFTVVFCFSGAHCPSPRQLQGLKALF

Target ORF information:

RefSeq Version	NM_001108413.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CDK5 regulatory subunit associated protein 1-like 1 (Cdkal1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108413.1

ORF Insert Sequence:

ATGCCTTCTG CATCCTGTGA TGTACTGCTG GATGACATAG AAGATATCAT ATCACAGGAA 
GATTCAAAGC CACAAGATAG ACATTTCTCA AGAAAGCATG TGTTTCCTAA AGTACGGAGG 
CGGAATACCC AAAAATATTT GCAAGAAGAG CCCAGCCCAC CAAGTGACAG CACGATTCCA 
GGCATACAGA AAATTTGGAT ACGAACATGG GGCTGCTCAC ATAATAATTC AGATGGAGAA 
TACATGGCTG GACAGCTTGC TGCCTATGGC TATAAAATTA CAGCTCAGTG TGGAAAAAGC 
CAGGCGTGGG CCTCATGGAA ATCATTCACC GTTGTCTTCT GTTTCTCAGG GGCTCACTGT 
CCTTCACCCC GTCAGCTTCA GGGTCTGAAA GCACTCTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CDK5 regulatory subunit-associated protein 1-like 1 (CDKAL1) is a tail-anchored protein in the endoplasmic reticulum (ER) of insulinoma cells.
The Journal of biological chemistry287(50)41808-19(2012 Dec)
Brambillasca S,Altkrueger A,Colombo SF,Friederich A,Eickelmann P,Mark M,Borgese N,Solimena M

Deficit of tRNA(Lys) modification by Cdkal1 causes the development of type 2 diabetes in mice.
The Journal of clinical investigation121(9)3598-608(2011 Sep)
Wei FY,Suzuki T,Watanabe S,Kimura S,Kaitsuka T,Fujimura A,Matsui H,Atta M,Michiue H,Fontecave M,Yamagata K,Suzuki T,Tomizawa K

Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosis.
PloS one5(12)e15553(2010 Dec)
Ohara-Imaizumi M,Yoshida M,Aoyagi K,Saito T,Okamura T,Takenaka H,Akimoto Y,Nakamichi Y,Takanashi-Yanobu R,Nishiwaki C,Kawakami H,Kato N,Hisanaga S,Kakei M,Nagamatsu S

Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
The Journal of biological chemistry285(37)28425-33(2010 Sep)
Arragain S,Handelman SK,Forouhar F,Wei FY,Tomizawa K,Hunt JF,Douki T,Fontecave M,Mulliez E,Atta M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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Cdkal1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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