Pf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pf4
Entrez Gene ID	360918
Full Name	platelet factor 4
Synonyms	Cxcl4,PF-4,Pf4a,RATPF4A
General protein information	Preferred Names platelet factor 4 Names platelet factor 4 C-X-C motif chemokine 4 chemokine (C-X-C motif) ligand 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	member of low-molecular weight chemokines superfamily; may mediate inflammation and immune response [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007729.1	NP_001007730.1	platelet factor 4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PF4	NP_002610.1
Pan troglodytes (chimpanzee)	PF4	XP_001155980.1
	PF4	XP_001102788.1
Bos taurus (cattle)	PF4	NP_001094532.1
Mus musculus (house mouse)	Pf4	NP_064316.1
Rattus norvegicus (Norway rat)	Pf4	NP_001007730.1

Gene Ontology
Bibliography

Related articles in PubMed

CXCL4 Contributes to the Pathogenesis of Chronic Liver Allograft Dysfunction.
Li J, Liu B, Shi Y, Xie KL, Yin HF, Yan LN, Lau WY, Wang GL
Journal of immunology research20169276986(2016)

Platelet factor 4 activity against P. falciparum and its translation to nonpeptidic mimics as antimalarials.
Love MS, Millholland MG, Mishra S, Kulkarni S, Freeman KB, Pan W, Kavash RW, Costanzo MJ, Jo H, Daly TM, Williams DR, Kowalska MA, Bergman LW, Poncz M, DeGrado WF, Sinnis P, Scott RW, Greenbaum DC
Cell host & microbe12(6)815-23(2012 Dec)

Induction of dendritic cell-like phenotype in macrophages during foam cell formation.
Cho HJ, Shashkin P, Gleissner CA, Dunson D, Jain N, Lee JK, Miller Y, Ley K
Physiological genomics29(2)149-60(2007 Apr)

Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction.
Wang X, Hsu MY, Steinbacher TE, Monticello TM, Schumacher WA
Thrombosis research119(5)593-600(2007)

Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes.
Okada Y, Matsuura E, Tozuka Z, Nagai R, Watanabe A, Matsumoto K, Yasui K, Jackman RW, Nakano T, Doi T
Blood104(7)2027-34(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified the informative Chronic Liver Allograft Dysfunction (CLAD)-associated genes; CXCL4, CXCR3, EGFR, JAK2, STAT3, and Collagen IV were associated with CLAD pathogenesis; validated that CXCL4 is upstream of these informative genes in the isolated hepatic stellate cells
Title: CXCL4 Contributes to the Pathogenesis of Chronic Liver Allograft Dysfunction.

The levels of PF-4 (as a platelet marker) mRNA were significantly higher in FeCl(2)-VT (2(-DeltaDeltaCT)=7.8) than in stasis-VT (2(-DeltaDeltaCT)=4.2, p<0.05).
Title: Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction.

PF4 promoters are transactivated by USF1 and 2 which may play an important role in megakaryocytic gene expression
Title: Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes.

this study identified a novel regulatory element,TME(tandem repeat of MEIS1 binding element)in the PF4 promoter and demonstrated that MEIS1/PBX complexes bind to the TME
Title: Homeodomain proteins MEIS1 and PBXs regulate the lineage-specific transcription of the platelet factor 4 gene.

A carboxyl-terminal fragment of this protein effects a rapid histamine release from rat peritoneal mast cells.
Title: Platelet factor 4 fragment induces histamine release from rat peritoneal mast cells.

The following Pf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11874	NM_001007729.1 Latest version!	Rattus norvegicus platelet factor 4 (Pf4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pf4 ( NM_001007729.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007730.1 Rattus norvegicus platelet factor 4 (Pf4), mRNA.

Price & Availability↑

CloneID	ORa11874
Clone ID Related Accession (Same CDS sequence)	NM_001007729.1
Accession Version	NM_001007729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	platelet factor 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M15254.1. On Nov 29, 2004 this sequence version replaced XM_341192.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF112725.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGCCG CTGCGGTGTT TCGAGGCCTC CGGCCCAGCC CTGAGCTGCT TCTTCTGGGT 
CTGCTGTTGC TGCCAGCTGT GGTTGCTGTC ACCAGGGCTA GTCCTGAAGA AAGCGACGGA 
GATCTTAGCT GTGTGTGTGT GAAGACCAGT TCTTCCAGGA TCCATCTCAA ACGCATCACC 
AGCCTGGAGG TGATCAAAGC AGGACCCCAC TGTGCGGTTC CCCAGCTCAT AGCCACGCTG 
AAGAATGGGA GCAAAATTTG CCTGGACCGG CAAGTACCTC TGTATAAGAA AATAATCAAG 
AAACTCCTGG AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007730.1
CDS	1..318
Translation	MSAAAVFRGLRPSPELLLLGLLLLPAVVAVTRASPEESDGDLSCVCVKTSSSRIHLKRITSLEVIKAGPHCAVPQLIATLKNGSKICLDRQVPLYKKIIKKLLES

Target ORF information:

RefSeq Version	NM_001007729.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus platelet factor 4 (Pf4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007729.1

ORF Insert Sequence:

ATGAGTGCCG CTGCGGTGTT TCGAGGCCTC CGGCCCAGCC CTGAGCTGCT TCTTCTGGGT 
CTGCTGTTGC TGCCAGCTGT GGTTGCTGTC ACCAGGGCTA GTCCTGAAGA AAGCGACGGA 
GATCTTAGCT GTGTGTGTGT GAAGACCAGT TCTTCCAGGA TCCATCTCAA ACGCATCACC 
AGCCTGGAGG TGATCAAAGC AGGACCCCAC TGTGCGGTTC CCCAGCTCAT AGCCACGCTG 
AAGAATGGGA GCAAAATTTG CCTGGACCGG CAAGTACCTC TGTATAAGAA AATAATCAAG 
AAACTCCTGG AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CXCL4 Contributes to the Pathogenesis of Chronic Liver Allograft Dysfunction.
Journal of immunology research20169276986(2016)
Li J,Liu B,Shi Y,Xie KL,Yin HF,Yan LN,Lau WY,Wang GL

Platelet factor 4 activity against P. falciparum and its translation to nonpeptidic mimics as antimalarials.
Cell host & microbe12(6)815-23(2012 Dec)
Love MS,Millholland MG,Mishra S,Kulkarni S,Freeman KB,Pan W,Kavash RW,Costanzo MJ,Jo H,Daly TM,Williams DR,Kowalska MA,Bergman LW,Poncz M,DeGrado WF,Sinnis P,Scott RW,Greenbaum DC

Induction of dendritic cell-like phenotype in macrophages during foam cell formation.
Physiological genomics29(2)149-60(2007 Apr)
Cho HJ,Shashkin P,Gleissner CA,Dunson D,Jain N,Lee JK,Miller Y,Ley K

Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction.
Thrombosis research119(5)593-600(2007)
Wang X,Hsu MY,Steinbacher TE,Monticello TM,Schumacher WA

Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes.
Blood104(7)2027-34(2004 Oct)
Okada Y,Matsuura E,Tozuka Z,Nagai R,Watanabe A,Matsumoto K,Yasui K,Jackman RW,Nakano T,Doi T

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Pf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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