ORF » Species Summary » Rattus norvegicus » Rfwd2 cDNA ORF clone

Rfwd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Rfwd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rfwd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa07285 NM_001025126.1
Latest version!		 Rattus norvegicus COP1, E3 ubiquitin ligase (Cop1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $258.30
$369.00
ORa35530 XM_006250113.3
Latest version!		 Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
ORa35531 XM_006250114.3
Latest version!		 Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    Rfwd2 ( NM_001025126.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020297.1 Rattus norvegicus COP1, E3 ubiquitin ligase (Cop1), mRNA.
    Price & Availability↑
    CloneID ORa07285
    Clone ID Related Accession (Same CDS sequence) NM_001025126.1
    Accession Version NM_001025126.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-27
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase RFWD2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091284.1. On Jul 1, 2005 this sequence version replaced XM_341137.2. ##Evidence-Data-START## Transcript exon combination :: BC091284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGTGGCT TGTACTCCCC CGTCAGTGAA GATAGCACGG TGCCTCAATT TGAAGCTCCT 
    TCTCCATCGC ACAGTAGCAT TATTGACTCT ACAGAATACA GCCAACCTCC AGGCTTCAGT 
    GGGCCGTCTC AGACAAAGAA ACAGCCTTGG TATAACAGCA CATTAGCATC AAGACGAAAG 
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAGTGTTATT TTTCTACAAG GATGTCTCGT 
    ATCTCAGATG ACAGTAGAAC TGCAAGCCAG TTAGATGAAT TTCAAGAATG CTTGTCCAAA 
    TTTACTCGAT ATAACTCTGT AAGACCGTTG GCCACATTGT CTTATGCTAG TGATCTGTAT 
    AATGGTTCCA GCATAGTTTC TAGTATTGAA TTTGACCGGG ATTGTGACTA CTTTGCAATT 
    GCTGGAGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATCCA GGATGCAGTG 
    GATATTCATT ACCCTGAGAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
    AGTAGTTACC ATAAGAACCT GCTAGCCAGC AGTGATTATG AAGGCACCGT TATACTATGG 
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAGC ATGAAAAGAG GTGTTGGAGT 
    GTCGACTTTA ATTTGATGGA TCCCAAACTT CTGGCTTCAG GTTCTGATGA TGCAAAAGTG 
    AAGCTTTGGT CCACCAATTT AGACAATTCT GTGGCAAGCA TCGAGGCAAA AGCTAATGTG 
    TGTTGTGTGA AGTTCAGCCC CTCCTCCAGG TACCATCTGG CTTTTGGCTG TGCAGATCAC 
    TGTGTCCACT ACTACGATCT TCGTAACACT AAACAGCCAA TAATGGTGTT CAAGGGGCAC 
    CGAAAAGCAG TGTCTTATGC CAAGTTTGTG AGTGGTGAAG AAATTGTCTC TGCCTCAACA 
    GACAGCCAAC TAAAACTGTG GAATGTGGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
    CATATTAATG AAAAAAACTT TGTAGGCCTT GCTTCCAATG GAGATTATAT AGCTTGTGGA 
    AGTGAGAACA ACTCCCTCTA CCTGTACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
    AAGTTTGATA CAGTTAAGAG TGTATTAGAT AAAGACCGGA AAGAAGATGA CACCAATGAA 
    TTTGTCAGTG CTGTGTGTTG GCGGGCACTA TCAGATGGGG AGTCCAATGT GCTGATTGCT 
    GCTAACAGCC AGGGTACAAT TAAGGTGCTA GAATTGGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020297.1
    CDS184..1485
    Translation

    Target ORF information:

    RefSeq Version NM_001025126.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus COP1, E3 ubiquitin ligase (Cop1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001025126.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGTGGCT TGTACTCCCC CGTCAGTGAA GATAGCACGG TGCCTCAATT TGAAGCTCCT 
    TCTCCATCGC ACAGTAGCAT TATTGACTCT ACAGAATACA GCCAACCTCC AGGCTTCAGT 
    GGGCCGTCTC AGACAAAGAA ACAGCCTTGG TATAACAGCA CATTAGCATC AAGACGAAAG 
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAGTGTTATT TTTCTACAAG GATGTCTCGT 
    ATCTCAGATG ACAGTAGAAC TGCAAGCCAG TTAGATGAAT TTCAAGAATG CTTGTCCAAA 
    TTTACTCGAT ATAACTCTGT AAGACCGTTG GCCACATTGT CTTATGCTAG TGATCTGTAT 
    AATGGTTCCA GCATAGTTTC TAGTATTGAA TTTGACCGGG ATTGTGACTA CTTTGCAATT 
    GCTGGAGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATCCA GGATGCAGTG 
    GATATTCATT ACCCTGAGAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
    AGTAGTTACC ATAAGAACCT GCTAGCCAGC AGTGATTATG AAGGCACCGT TATACTATGG 
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAGC ATGAAAAGAG GTGTTGGAGT 
    GTCGACTTTA ATTTGATGGA TCCCAAACTT CTGGCTTCAG GTTCTGATGA TGCAAAAGTG 
    AAGCTTTGGT CCACCAATTT AGACAATTCT GTGGCAAGCA TCGAGGCAAA AGCTAATGTG 
    TGTTGTGTGA AGTTCAGCCC CTCCTCCAGG TACCATCTGG CTTTTGGCTG TGCAGATCAC 
    TGTGTCCACT ACTACGATCT TCGTAACACT AAACAGCCAA TAATGGTGTT CAAGGGGCAC 
    CGAAAAGCAG TGTCTTATGC CAAGTTTGTG AGTGGTGAAG AAATTGTCTC TGCCTCAACA 
    GACAGCCAAC TAAAACTGTG GAATGTGGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
    CATATTAATG AAAAAAACTT TGTAGGCCTT GCTTCCAATG GAGATTATAT AGCTTGTGGA 
    AGTGAGAACA ACTCCCTCTA CCTGTACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
    AAGTTTGATA CAGTTAAGAG TGTATTAGAT AAAGACCGGA AAGAAGATGA CACCAATGAA 
    TTTGTCAGTG CTGTGTGTTG GCGGGCACTA TCAGATGGGG AGTCCAATGT GCTGATTGCT 
    GCTAACAGCC AGGGTACAAT TAAGGTGCTA GAATTGGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Rfwd2 ( XM_006250113.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250175.1 Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ORa35530
    Clone ID Related Accession (Same CDS sequence) XM_006250113.3
    Accession Version XM_006250113.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCAGGTA GCCGCCAGGC CGGTTCGGGT TCCGCTGGGA CGAGCCCTGG CTCCTCAGCC 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTATCCTCGT CTCCGTCGCC GCCTTCCGTG 
    GCGGCCTCGG CGGCGACGCT GGTGTCCGGC GGGGTAGCTC CAGCCGCGGG CTCCGGCGGC 
    CTCGGCGGCC CGGGGCGGCC TGTGCTAGTG GCGGCCGCGG TGTCTGGCAG CGCGAGCGCC 
    GGCGGGGCAG TGTCCGCGGG CCTGTCCCGG CTCAGCTGCG CTGCCAGGCC CAGCGCCGGC 
    GTTGGAGGGA GCAGCTCCAG CCTCGGCAGC AGCAGTAGGA AGCGACCTCT GATCGCCCCG 
    CTTTGCAACG GGCTCCTCAA CTCCTACGAG GACAAAAGCA ACGACTTCGT CTGCCCCATC 
    TGCTTTGATA TGATTGAAGA GGCATACATG ACAAAATGTG GCCACAGCTT TTGCTACAAG 
    TGTATTCATC AGAGTTTGGA GGACAATAAT AGATGTCCCA AGTGTAATTA TGTTGTGGAC 
    AATATCGACC ATCTCTATCC TAATTTCTTG GTGAATGAAC TCATTCTCAA ACAGAAGCAA 
    AGATTTGAGG AAAAGAGGTT CAAATTGGAC CACTCAGTGA GTAGCACCAA TGGGCATAGG 
    TGGCAAATAT TTCAAGATCT GCTAGGAACT GATCAAGATA ATCTTGATTT GGCTAATGTC 
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAAAAACAAC TGGAAGCAGA ATCACATGCA 
    GCTCAGCTAC AGATCCTTAT GGAATTCCTC AAGGTTGCCA GGAGAAATAA GAGAGAGCAA 
    TTGGAACAGA TCCAGAAGGA ACTAAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAG 
    ATGAGTGGCT TGTACTCCCC CGTCAGTGAA GATAGCACGG TGCCTCAATT TGAAGCTCCT 
    TCTCCATCGC ACAGTAGCAT TATTGACTCT ACAGAATACA GCCAACCTCC AGGCTTCAGT 
    GGGCCGTCTC AGACAAAGAA ACAGCCTTGG TATAACAGCA CATTAGCATC AAGACGAAAG 
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAGTGTTATT TTTCTACAAG GATGTCTCGT 
    ATCTCAGATG ACAGTAGAAC TGCAAGCCAG TTAGATGAAT TTCAAGAATG CTTGTCCAAA 
    TTTACTCGAT ATAACTCTGT AAGACCGTTG GCCACATTGT CTTATGCTAG TGATCTGTAT 
    AATGGTTCCA GCATAGTTTC TAGTATTGAA TTTGACCGGG ATTGTGACTA CTTTGCAATT 
    GCTGGAGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATCCA GGATGCAGTG 
    GATATTCATT ACCCTGAGAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
    AGTAGTTACC ATAAGAACCT GCTAGCCAGC AGTGATTATG AAGGCACCGT TATACTATGG 
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAGC ATGAAAAGAG GTGTTGGAGT 
    GTCGACTTTA ATTTGATGGA TCCCAAACTT CTGGCTTCAG GTTCTGATGA TGCAAAAGTG 
    AAGCTTTGGT CCACCAATTT AGACAATTCT GTGGCAAGCA TCGAGGCAAA AGCTAATGTG 
    TGTTGTGTGA AGTTCAGCCC CTCCTCCAGG TACCATCTGG CTTTTGGCTG TGCAGATCAC 
    TGTGTCCACT ACTACGATCT TCGTAACACT AAACAGCCAA TAATGGTGTT CAAGGGGCAC 
    CGAAAAGCAG TGTCTTATGC CAAGTTTGTG AGTGGTGAAG AAATTGTCTC TGCCTCAACA 
    GACAGCCAAC TAAAACTGTG GAATGTGGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
    CATATTAATG AAAAAAACTT TGTAGGCCTT GCTTCCAATG GAGATTATAT AGCTTGTGGA 
    AGTGAGAACA ACTCCCTCTA CCTGTACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
    AAGTTTGATA CAGTTAAGAG TGTATTAGAT AAAGACCGGA AAGAAGATGA CACCAATGAA 
    TTTGTCAGTG CTGTGTGTTG GCGGGCACTA TCAGATGGGG AGTCCAATGT GCTGATTGCT 
    GCTAACAGCC AGGGTACAAT TAAGGTGCTA GAATTGGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006250175.1
    CDS533..2734
    Translation

    Target ORF information:

    RefSeq Version XM_006250113.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006250113.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCAGGTA GCCGCCAGGC CGGTTCGGGT TCCGCTGGGA CGAGCCCTGG CTCCTCAGCC 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTATCCTCGT CTCCGTCGCC GCCTTCCGTG 
    GCGGCCTCGG CGGCGACGCT GGTGTCCGGC GGGGTAGCTC CAGCCGCGGG CTCCGGCGGC 
    CTCGGCGGCC CGGGGCGGCC TGTGCTAGTG GCGGCCGCGG TGTCTGGCAG CGCGAGCGCC 
    GGCGGGGCAG TGTCCGCGGG CCTGTCCCGG CTCAGCTGCG CTGCCAGGCC CAGCGCCGGC 
    GTTGGAGGGA GCAGCTCCAG CCTCGGCAGC AGCAGTAGGA AGCGACCTCT GATCGCCCCG 
    CTTTGCAACG GGCTCCTCAA CTCCTACGAG GACAAAAGCA ACGACTTCGT CTGCCCCATC 
    TGCTTTGATA TGATTGAAGA GGCATACATG ACAAAATGTG GCCACAGCTT TTGCTACAAG 
    TGTATTCATC AGAGTTTGGA GGACAATAAT AGATGTCCCA AGTGTAATTA TGTTGTGGAC 
    AATATCGACC ATCTCTATCC TAATTTCTTG GTGAATGAAC TCATTCTCAA ACAGAAGCAA 
    AGATTTGAGG AAAAGAGGTT CAAATTGGAC CACTCAGTGA GTAGCACCAA TGGGCATAGG 
    TGGCAAATAT TTCAAGATCT GCTAGGAACT GATCAAGATA ATCTTGATTT GGCTAATGTC 
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAAAAACAAC TGGAAGCAGA ATCACATGCA 
    GCTCAGCTAC AGATCCTTAT GGAATTCCTC AAGGTTGCCA GGAGAAATAA GAGAGAGCAA 
    TTGGAACAGA TCCAGAAGGA ACTAAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAG 
    ATGAGTGGCT TGTACTCCCC CGTCAGTGAA GATAGCACGG TGCCTCAATT TGAAGCTCCT 
    TCTCCATCGC ACAGTAGCAT TATTGACTCT ACAGAATACA GCCAACCTCC AGGCTTCAGT 
    GGGCCGTCTC AGACAAAGAA ACAGCCTTGG TATAACAGCA CATTAGCATC AAGACGAAAG 
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAGTGTTATT TTTCTACAAG GATGTCTCGT 
    ATCTCAGATG ACAGTAGAAC TGCAAGCCAG TTAGATGAAT TTCAAGAATG CTTGTCCAAA 
    TTTACTCGAT ATAACTCTGT AAGACCGTTG GCCACATTGT CTTATGCTAG TGATCTGTAT 
    AATGGTTCCA GCATAGTTTC TAGTATTGAA TTTGACCGGG ATTGTGACTA CTTTGCAATT 
    GCTGGAGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATCCA GGATGCAGTG 
    GATATTCATT ACCCTGAGAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
    AGTAGTTACC ATAAGAACCT GCTAGCCAGC AGTGATTATG AAGGCACCGT TATACTATGG 
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAGC ATGAAAAGAG GTGTTGGAGT 
    GTCGACTTTA ATTTGATGGA TCCCAAACTT CTGGCTTCAG GTTCTGATGA TGCAAAAGTG 
    AAGCTTTGGT CCACCAATTT AGACAATTCT GTGGCAAGCA TCGAGGCAAA AGCTAATGTG 
    TGTTGTGTGA AGTTCAGCCC CTCCTCCAGG TACCATCTGG CTTTTGGCTG TGCAGATCAC 
    TGTGTCCACT ACTACGATCT TCGTAACACT AAACAGCCAA TAATGGTGTT CAAGGGGCAC 
    CGAAAAGCAG TGTCTTATGC CAAGTTTGTG AGTGGTGAAG AAATTGTCTC TGCCTCAACA 
    GACAGCCAAC TAAAACTGTG GAATGTGGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
    CATATTAATG AAAAAAACTT TGTAGGCCTT GCTTCCAATG GAGATTATAT AGCTTGTGGA 
    AGTGAGAACA ACTCCCTCTA CCTGTACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
    AAGTTTGATA CAGTTAAGAG TGTATTAGAT AAAGACCGGA AAGAAGATGA CACCAATGAA 
    TTTGTCAGTG CTGTGTGTTG GCGGGCACTA TCAGATGGGG AGTCCAATGT GCTGATTGCT 
    GCTAACAGCC AGGGTACAAT TAAGGTGCTA GAATTGGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Rfwd2 ( XM_006250114.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250176.1 Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ORa35531
    Clone ID Related Accession (Same CDS sequence) XM_006250114.3
    Accession Version XM_006250114.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCAGGTA GCCGCCAGGC CGGTTCGGGT TCCGCTGGGA CGAGCCCTGG CTCCTCAGCC 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTATCCTCGT CTCCGTCGCC GCCTTCCGTG 
    GCGGCCTCGG CGGCGACGCT GGTGTCCGGC GGGGTAGCTC CAGCCGCGGG CTCCGGCGGC 
    CTCGGCGGCC CGGGGCGGCC TGTGCTAGTG GCGGCCGCGG TGTCTGGCAG CGCGAGCGCC 
    GGCGGGGCAG TGTCCGCGGG CCTGTCCCGG CTCAGCTGCG CTGCCAGGCC CAGCGCCGGC 
    GTTGGAGGGA GCAGCTCCAG CCTCGGCAGC AGCAGTAGGA AGCGACCTCT GATCGCCCCG 
    CTTTGCAACG GGCTCCTCAA CTCCTACGAG GACAAAAGCA ACGACTTCGT CTGCCCCATC 
    TGCTTTGATA TGATTGAAGA GGCATACATG ACAAAATGTG GCCACAGCTT TTGCTACAAG 
    TGTATTCATC AGAGTTTGGA GGACAATAAT AGATGTCCCA AGTGTAATTA TGTTGTGGAC 
    AATATCGACC ATCTCTATCC TAATTTCTTG GTGAATGAAC TCATTCTCAA ACAGAAGCAA 
    AGATTTGAGG AAAAGAGGTT CAAATTGGAC CACTCAAATG GGCATAGGTG GCAAATATTT 
    CAAGATCTGC TAGGAACTGA TCAAGATAAT CTTGATTTGG CTAATGTCAA CCTCATGTTG 
    GAATTACTAG TGCAGAAGAA AAAACAACTG GAAGCAGAAT CACATGCAGC TCAGCTACAG 
    ATCCTTATGG AATTCCTCAA GGTTGCCAGG AGAAATAAGA GAGAGCAATT GGAACAGATC 
    CAGAAGGAAC TAAGTGTTTT GGAAGAGGAT ATTAAAAGAG TGGAAGAGAT GAGTGGCTTG 
    TACTCCCCCG TCAGTGAAGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCGCAC 
    AGTAGCATTA TTGACTCTAC AGAATACAGC CAACCTCCAG GCTTCAGTGG GCCGTCTCAG 
    ACAAAGAAAC AGCCTTGGTA TAACAGCACA TTAGCATCAA GACGAAAGCG ACTCACTGCT 
    CATTTTGAAG ACTTAGAGCA GTGTTATTTT TCTACAAGGA TGTCTCGTAT CTCAGATGAC 
    AGTAGAACTG CAAGCCAGTT AGATGAATTT CAAGAATGCT TGTCCAAATT TACTCGATAT 
    AACTCTGTAA GACCGTTGGC CACATTGTCT TATGCTAGTG ATCTGTATAA TGGTTCCAGC 
    ATAGTTTCTA GTATTGAATT TGACCGGGAT TGTGACTACT TTGCAATTGC TGGAGTTACA 
    AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATCCAGG ATGCAGTGGA TATTCATTAC 
    CCTGAGAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT 
    AAGAACCTGC TAGCCAGCAG TGATTATGAA GGCACCGTTA TACTATGGGA TGGATTCACA 
    GGACAGAGGT CAAAGGTCTA TCAGGAGCAT GAAAAGAGGT GTTGGAGTGT CGACTTTAAT 
    TTGATGGATC CCAAACTTCT GGCTTCAGGT TCTGATGATG CAAAAGTGAA GCTTTGGTCC 
    ACCAATTTAG ACAATTCTGT GGCAAGCATC GAGGCAAAAG CTAATGTGTG TTGTGTGAAG 
    TTCAGCCCCT CCTCCAGGTA CCATCTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC 
    TACGATCTTC GTAACACTAA ACAGCCAATA ATGGTGTTCA AGGGGCACCG AAAAGCAGTG 
    TCTTATGCCA AGTTTGTGAG TGGTGAAGAA ATTGTCTCTG CCTCAACAGA CAGCCAACTA 
    AAACTGTGGA ATGTGGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA TATTAATGAA 
    AAAAACTTTG TAGGCCTTGC TTCCAATGGA GATTATATAG CTTGTGGAAG TGAGAACAAC 
    TCCCTCTACC TGTACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACA 
    GTTAAGAGTG TATTAGATAA AGACCGGAAA GAAGATGACA CCAATGAATT TGTCAGTGCT 
    GTGTGTTGGC GGGCACTATC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGCCAG 
    GGTACAATTA AGGTGCTAGA ATTGGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006250176.1
    CDS533..2722
    Translation

    Target ORF information:

    RefSeq Version XM_006250114.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006250114.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCAGGTA GCCGCCAGGC CGGTTCGGGT TCCGCTGGGA CGAGCCCTGG CTCCTCAGCC 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTATCCTCGT CTCCGTCGCC GCCTTCCGTG 
    GCGGCCTCGG CGGCGACGCT GGTGTCCGGC GGGGTAGCTC CAGCCGCGGG CTCCGGCGGC 
    CTCGGCGGCC CGGGGCGGCC TGTGCTAGTG GCGGCCGCGG TGTCTGGCAG CGCGAGCGCC 
    GGCGGGGCAG TGTCCGCGGG CCTGTCCCGG CTCAGCTGCG CTGCCAGGCC CAGCGCCGGC 
    GTTGGAGGGA GCAGCTCCAG CCTCGGCAGC AGCAGTAGGA AGCGACCTCT GATCGCCCCG 
    CTTTGCAACG GGCTCCTCAA CTCCTACGAG GACAAAAGCA ACGACTTCGT CTGCCCCATC 
    TGCTTTGATA TGATTGAAGA GGCATACATG ACAAAATGTG GCCACAGCTT TTGCTACAAG 
    TGTATTCATC AGAGTTTGGA GGACAATAAT AGATGTCCCA AGTGTAATTA TGTTGTGGAC 
    AATATCGACC ATCTCTATCC TAATTTCTTG GTGAATGAAC TCATTCTCAA ACAGAAGCAA 
    AGATTTGAGG AAAAGAGGTT CAAATTGGAC CACTCAAATG GGCATAGGTG GCAAATATTT 
    CAAGATCTGC TAGGAACTGA TCAAGATAAT CTTGATTTGG CTAATGTCAA CCTCATGTTG 
    GAATTACTAG TGCAGAAGAA AAAACAACTG GAAGCAGAAT CACATGCAGC TCAGCTACAG 
    ATCCTTATGG AATTCCTCAA GGTTGCCAGG AGAAATAAGA GAGAGCAATT GGAACAGATC 
    CAGAAGGAAC TAAGTGTTTT GGAAGAGGAT ATTAAAAGAG TGGAAGAGAT GAGTGGCTTG 
    TACTCCCCCG TCAGTGAAGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCGCAC 
    AGTAGCATTA TTGACTCTAC AGAATACAGC CAACCTCCAG GCTTCAGTGG GCCGTCTCAG 
    ACAAAGAAAC AGCCTTGGTA TAACAGCACA TTAGCATCAA GACGAAAGCG ACTCACTGCT 
    CATTTTGAAG ACTTAGAGCA GTGTTATTTT TCTACAAGGA TGTCTCGTAT CTCAGATGAC 
    AGTAGAACTG CAAGCCAGTT AGATGAATTT CAAGAATGCT TGTCCAAATT TACTCGATAT 
    AACTCTGTAA GACCGTTGGC CACATTGTCT TATGCTAGTG ATCTGTATAA TGGTTCCAGC 
    ATAGTTTCTA GTATTGAATT TGACCGGGAT TGTGACTACT TTGCAATTGC TGGAGTTACA 
    AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATCCAGG ATGCAGTGGA TATTCATTAC 
    CCTGAGAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT 
    AAGAACCTGC TAGCCAGCAG TGATTATGAA GGCACCGTTA TACTATGGGA TGGATTCACA 
    GGACAGAGGT CAAAGGTCTA TCAGGAGCAT GAAAAGAGGT GTTGGAGTGT CGACTTTAAT 
    TTGATGGATC CCAAACTTCT GGCTTCAGGT TCTGATGATG CAAAAGTGAA GCTTTGGTCC 
    ACCAATTTAG ACAATTCTGT GGCAAGCATC GAGGCAAAAG CTAATGTGTG TTGTGTGAAG 
    TTCAGCCCCT CCTCCAGGTA CCATCTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC 
    TACGATCTTC GTAACACTAA ACAGCCAATA ATGGTGTTCA AGGGGCACCG AAAAGCAGTG 
    TCTTATGCCA AGTTTGTGAG TGGTGAAGAA ATTGTCTCTG CCTCAACAGA CAGCCAACTA 
    AAACTGTGGA ATGTGGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA TATTAATGAA 
    AAAAACTTTG TAGGCCTTGC TTCCAATGGA GATTATATAG CTTGTGGAAG TGAGAACAAC 
    TCCCTCTACC TGTACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACA 
    GTTAAGAGTG TATTAGATAA AGACCGGAAA GAAGATGACA CCAATGAATT TGTCAGTGCT 
    GTGTGTTGGC GGGCACTATC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGCCAG 
    GGTACAATTA AGGTGCTAGA ATTGGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

見積依頼

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