Top2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top2a
Entrez Gene ID	360243
Full Name	DNA topoisomerase II alpha
General protein information	Preferred Names DNA topoisomerase II alpha Names DNA topoisomerase 2-alpha DNA topoisomerase II, alpha isozyme topoisomerase (DNA) 2 alpha topoisomerase (DNA) II alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	DNA topoisomerase, a nuclear enzyme that can alter the topologic state of DNA during transcription [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022183.2	NP_071519.2	DNA topoisomerase 2-alpha

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Top2a	NP_035753.2
Rattus norvegicus (Norway rat)	Top2a	NP_071519.2
Xenopus tropicalis (tropical clawed frog)	topoii	XP_002942632.2
Danio rerio (zebrafish)	top2a	NP_001003834.1
Drosophila melanogaster (fruit fly)	Top2	NP_001260580.1
Caenorhabditis elegans (roundworm)	top-2	NP_496536.1
Bos taurus (cattle)	TOP2A	XP_002696055.2
Gallus gallus (chicken)	TOP2A	NP_990122.1
Homo sapiens (human)	TOP2A	NP_001058.2
Pan troglodytes (chimpanzee)	TOP2A	XP_003315524.1
Canis lupus familiaris (dog)	TOP2A	XP_537646.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008917	XP_319665.4
	TOP2A	XP_002808175.1

Gene Ontology
Bibliography

Related articles in PubMed

DNA-binding activity of rat DNA topoisomerase II ? C-terminal domain contributes to efficient DNA catenation in vitro.
Kawano S, Kato Y, Okada N, Sano K, Tsutsui K, Tsutsui KM, Ikeda S
Journal of biochemistry159(3)363-9(2016 Mar)

Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase II?: A story behind the molecular modeling approach.
Mladenovi? M, Stankovi? N, Mati? S, Stani? S, Mihailovi? M, Mihailovi? V, Katani? J, Boroja T, Vukovi? N
Biochemical pharmacology98(1)243-66(2015 Nov)

Anthracyclines/trastuzumab: new aspects of cardiotoxicity and molecular mechanisms.
Rochette L, Guenancia C, Gudjoncik A, Hachet O, Zeller M, Cottin Y, Vergely C
Trends in pharmacological sciences36(6)326-48(2015 Jun)

Condensin confers the longitudinal rigidity of chromosomes.
Houlard M, Godwin J, Metson J, Lee J, Hirano T, Nasmyth K
Nature cell biology17(6)771-81(2015 Jun)

Shikonin shortens the circadian period: possible involvement of Top2 inhibition.
Ogawa Y, Kawano Y, Yamazaki Y, Onishi Y
Biochemical and biophysical research communications443(1)339-43(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C-terminal domain of rat topo IIalpha is associated with efficient DNA catenation in vitro, based on comparison of wild-type rat topo IIalpha and its deletion mutants.
Title: DNA-binding activity of rat DNA topoisomerase II ? C-terminal domain contributes to efficient DNA catenation in vitro.

The genotoxic and antigenotoxic effects of 8 chroman-2,4-diones are reported; the role of their TOPIIalpha inhibitory effect is described.
Title: Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase II?: A story behind the molecular modeling approach.

Data indicate that dexrazoxane may protect cardiomyocytes via its catalytic topoisomerase II (TOP2) inhibitory activity.
Title: Catalytic inhibitors of topoisomerase II differently modulate the toxicity of anthracyclines in cardiac and cancer cells.

Rac1-regulated mechanisms are required for DNA damage induction and subsequent activation of the DDR following treatment with topo II but not topo I poisons
Title: Rac1 protein signaling is required for DNA damage response stimulated by topoisomerase II poisons.

The Topoisomerase II alpha is predominantly localized in proliferative tissue and found in embryos up to post-natal day 1.Topoisomerase II beta in brain showed highest activity in cerebellar region and that too only neuronal cell fraction.
Title: Analysis of age dependent changes of Topoisomerase II alpha and beta in rat brain.

The following Top2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05374	NM_022183.2 Latest version!	Rattus norvegicus DNA topoisomerase II alpha (Top2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top2a ( NM_022183.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071519.2 Rattus norvegicus DNA topoisomerase II alpha (Top2a), mRNA.

Price & Availability↑

CloneID	ORa05374
Clone ID Related Accession (Same CDS sequence)	NM_022183.2
Accession Version	NM_022183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 2-alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Z19552.1. On or before Dec 17, 2003 this sequence version replaced XM_346406.1, NM_022183.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z19552.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC TACTCAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGACT GTCCGTTGAA AGAATCTATC AGAAAAAAAC CCAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATC GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGTATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAAAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGCA TTAGAGTCAC AATGATGCGA AATAATTTAA TTAGTATATG GAATAATGGA 
AAGGGAATTC CTGTTGTTGA ACACAAAGTG GAGAAAATGT ATGTCCCAGC TCTCATATTT 
GGACAGCTCC TGACCTCCAG TAACTACGAT GATGATGAGA AGAAAGTGAC AGGTGGTCGA 
AATGGCTATG GAGCTAAACT GTGTAACATA TTCAGCACCA AATTTACTGT GGAAACAGCC 
AGTAGGGAAT ACAAGAAAAT GTTCAAACAG ACATGGATGG ATAACATGGG GAGAGCTGGT 
GACATGGAGC TCAAGCCCTT TAGCGGAGAG GATTACACGT GTATTACCTT CCAGCCTGAC 
TTATCTAAGT TTAAAATGCA AAGCCTGGAC AAAGATATTG TCGCACTGAT GGTCAGAAGA 
GCATACGATA TTGCTGGTTC CACCAAAGAT GTAAAAGTCT TTCTTAATGG AAACAGGCTG 
CCAGTCAAAG GGTTCCGTAG TTACGTGGAT ATGTATTTGA AGGATAAGGT TGATGAAACT 
GGGAACGCTC TGAAGGTGGT GCATGAACAA GTAAATCCCC GGTGGGAAGT GTGCTTAACA 
ATGAGCGAGA AAGGCTTTCA GCAGATTAGC TTCGTCAACA GCATCGCTAC TTCTAAGGGT 
GGCAGACATG TCGATTATGT AGCCGATCAG ATTGTGAGTA AACTCGTTGA TGTGGTGAAA 
AAGAAGAACA AGGGAGGTGT TGCAGTGAAG GCCGATCAGG TGAAGAATCA CATGTGGATT 
TTTGGAAATG CGGTAATTGA AAACCCAACC TTTGATTCTC AAACAAAAGA AAACATGACT 
TTACAAGCCA AGAGCTTTGG ATCAACTTGT CAATTAAGTG AAAAATTCAT CAAAGCTGCA 
ATTGGTTGTG GTATTGTCGA AAGCATACTA AACTGGGTGA AGTTTAAGGC CCAAATCCAA 
TTAAACAAGA AGTGTTCAGC TGTAAAACAT AACAGGATCA AGGGAATCCC CAAACTTGAC 
GACGCCAATG ACGCAGGGAG CCGAAACTCT GCTGAATGCA CACTTATCCT GACTGAAGGG 
GACTCAGCCA AAACTCTGGC AGTCTCAGGA CTTGGAGTGG TTGGAAGAGA CAAATATGGG 
GTGTTCCCTC TCAGAGGAAA AATACTCAAT GTGCGAGAAG CTTCTCATAA ACAGATCATG 
GAAAATGCTG AAATTAACAA TATCATCAAG ATTGTGGGGC TTCAGTACAA GAAAAACTAT 
GAGGATGAAG ATTCATTGAA AACTCTTCGT TATGGGAAGA TCATGATTAT GACAGATCAG 
GACCAAGATG GTTCCCACAT CAAAGGCTTG CTGATCAATT TTATCCATCA CAATTGGCCA 
TCTCTTCTGC GACATCGTTT TCTAGAAGAA TTTATCACTC CTATTGTAAA GGTATCTAAA 
AACAAGCAAG AAATAGCATT CTACAGTCTT CCCGAGTTTG AAGAATGGAA GAGTACAAAT 
CCAAATCACA AAAAATGGAA AGTCAAGTAC TACAAAGGTT TGGGCACGAG CACATCAAAG 
GAAGCTAAGG AGTATTTTGC AAATATGAAA CGACATCGTA TTCAGTTCAA ATACTCTGGC 
CCTGAAGACG ATGCTGCGAT CAGCCTGGCC TTTAGTAAAA AACAGGTCGA TGATCGAAAG 
GAATGGTTAA CTAATTTCAT GGAAGATAGA AGACAGCGGA AGTTACTTGG CCTTCCTGAG 
GATTATTTGT ATGGACAAAC CACTATGTAC CTGACCTATA ATGATTTCAT TAACAAGGAA 
CTTATCCTCT TTTCAAACTC TGATAATGAA AGATCTATCC CATCTATGGT AGATGGTTTG 
AAACCAGGTC AGAGAAAGGT TTTGTTTACT TGTTTCAAAC GGAATGACAA GCGAGAGGTG 
AAAGTTGCCC AGTTAGCTGG GTCAGTGGCA GAAATGTCCT CTTATCACCA TGGTGAGATG 
TCGCTGATGA TGACCATTAT CAATTTGGCT CAGAATTTTG TGGGTAGCAA TAATTTGAAC 
CTTCTACAGC CTATTGGTCA GTTTGGTACC AGGCTGCATG GTGGCAAGGA CTCAGCCAGC 
CCTAGGTACA TCTTTACAAT GCTCAGCCCT TTGGCTCGGT TGTTATTTCC ATCAAAAGAT 
GATCACACGT TACGGTTTCT GTATGACGAC AACCAGCGTG TTGAGCCTGA ATGGTACATC 
CCTATTATAC CCATGGTGCT GATAAATGGT GCTGAGGGAA TTGGTACTGG GTGGTCCTGC 
AAAATCCCCA ACTTTGATGT TCGTGAAGTC GTAAATAATA TCAGGCGGCT GTTGGATGGA 
GAAGAGCCTC TGCCCATGCT CCCAAGTTAT AAGAATTACA AAGGTACTAT TGAAGAACTG 
GCTTCAAATC AGTATGTGAT TAATGGAGAA GTAGCTATTC TGAATTCCAC AACCATTGAG 
ATCACTGAGC TTCCCATCAG AACATGGACC CAGACATATA AGGAACAGGT TCTAGAACCT 
ATGTTGAATG GCACCGAGAA GACACCCCCT CTAATAACAG ACTATAGGGA ATACCACACA 
GATACCACTG TGAAGTTTGT CATAAAGATG ACTGAAGAAA AATTGGCAGA GGCAGAGAGA 
GTGGGACTAC ACAAAGTCTT CAAACTCCAG ACTAGTCTCA CTTGCAACTC TATGGTACTT 
TTTGACCATG TTGGTTGTTT AAAGAAATAT GACACTGTGT TGGATATTCT AAGAGACTTC 
TTTGAGCTCA GGCTTAAATA TTATGGATTA AGAAAAGAAT GGCTTCTAGG AATGCTTGGT 
GCAGAATCTT CTAAACTGAA TAATCAAGCT CGCTTTATAT TAGAGAAAAT AGATGGCAAA 
ATAGTCATTG AAAATAAACC TAAGAAAGAA TTAATTAAAG TTCTGATTCA GAGAGGCTAT 
GACTCCGACC CTGTGAAAGC CTGGAAAGAA GCTCAGCAGA AGGTCCCAGA AGAAGAGGAA 
AATGAAGAGA ATGAAGAGAG CGAGAGTGAA AGCACGAGCC CTGCAGCGGA GTCTGGACCG 
ACCTTCAACT ACCTTCTTGA TATGCCCCTG TGGTATCTAA CCAAGGAGAA GAAGGACGAG 
CTGTGCAAGC AAAGGGACGA GAAGGAACAA GAGCTCAACA CGTTAAAGAA AAAGACTCCA 
TCGGATTTGT GGAAGGAAGA CCTGGCTGCT TTTGTGGAAG AACTGGAGGT TGTTGAAGCC 
AAGGAAAAAC AGGATGAACA AGTAGGACTT CCTGGAAAGG GGGTGAAAGC GAAGGGGAAG 
AAAGCGCAGA TATCTGAAGT CCTGCCTTCT CCTGTGGGGA AAAGGGTCAT CCCGCAGGTG 
ACTATGGAGA TGAGAGCTGA GGCAGAGAAG AAAATCAGGA GGAAAATTAA GAGCGAGAAT 
GTTGAGGGCA CCCCTGCTGA GGATGGTGCT GAGCCAGGAC TCCGGCAGAG ACTAGAGAAG 
AGGCAGAAGA GAGAGCCAGG TACCAGGGCA AAGAAGCAAA CTACATTGCC ATTTAAGCCT 
ATCAAAAAAG CCCAAAAGCA AAACCCTTGG TCTGATTCTG AATCAGATAT GAGCAGCAAC 
GAAAGCAATT TTGATGTCCC TCCACGAGAG AAAGAGCCAA GAATTGCTGC AACAAAAGCC 
AAATTCACGG CGGATTTAGA CTCTGATGAC GATTTCTCAG GCTTGGATGA GAAGGATGAG 
GATGAAGATT TCTTCCCATT AGATGATACT CCACCTAAGA CCAAAATGCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGCC ACAGAAGAGC TCCACATCAG TTGATCTTGA AAGTGATGGG 
AAAGACAGTG TACCAGCTTC TCCAGGCGCT TCTGCTGCTG ACGTCCCAGC TGAGACTGAA 
CCCTCCAAGC CGTCCTCCAA ACAGACAGTG GGAGTGAAGA GGACAATAAC CAAAGGTCAG 
TCTTTAACCT CCACTGCTGG CACCAAAAAG AGAGCTGTAC CAAAGGAGAC CAAGTCAGAT 
TCAGCCTTGA ATGCTCATGT CTCCAAAAAG CCTGCTCCTG CCAAAGCCAA GAACAGTCGC 
AAGAGGATGC CATCTTCTTC TGACAGCTCG GACTCTGAGT TTGAGAAGGC GATTTCTAAA 
GGTGCCACAA GCAAGAAACT CAAGGGAGAG GAGCGGGATT TCCATGTGGA CTTAGATGAC 
ACTGTGGCTC CTCGAGCAAA ATCCGGACGA GCAAGGAAGC CGATTAAGTA CCTGGAGGAG 
TCGGATGACG ACCTCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071519.2
CDS	1..4581
Translation	MEVSPLQPVNENMLLNKKKNEDGKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTMMRNNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNRLPVKGFRSYVDMYLKDKVDETGNALKVVHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKADQVKNHMWIFGNAVIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSAECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSTNPNHKKWKVKYYKGLGTSTSKEAKEYFANMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTMYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPSKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNYKGTIEELASNQYVINGEVAILNSTTIEITELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPEEEENEENEESESESTSPAAESGPTFNYLLDMPLWYLTKEKKDELCKQRDEKEQELNTLKKKTPSDLWKEDLAAFVEELEVVEAKEKQDEQVGLPGKGVKAKGKKAQISEVLPSPVGKRVIPQVTMEMRAEAEKKIRRKIKSENVEGTPAEDGAEPGLRQRLEKRQKREPGTRAKKQTTLPFKPIKKAQKQNPWSDSESDMSSNESNFDVPPREKEPRIAATKAKFTADLDSDDDFSGLDEKDEDEDFFPLDDTPPKTKMPPKNTKKALKPQKSSTSVDLESDGKDSVPASPGASAADVPAETEPSKPSSKQTVGVKRTITKGQSLTSTAGTKKRAVPKETKSDSALNAHVSKKPAPAKAKNSRKRMPSSSDSSDSEFEKAISKGATSKKLKGEERDFHVDLDDTVAPRAKSGRARKPIKYLEESDDDLF

Target ORF information:

RefSeq Version	NM_022183.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA topoisomerase II alpha (Top2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022183.2

ORF Insert Sequence:

ATGGAAGTGT CACCATTGCA GCCTGTAAAT GAAAATATGC TACTCAACAA AAAGAAGAAT 
GAAGATGGCA AGAAAAGACT GTCCGTTGAA AGAATCTATC AGAAAAAAAC CCAACTGGAA 
CATATACTGC TCCGCCCAGA TACCTACATC GGCTCTGTGG AGTTAGTGAC CCAGCAAATG 
TGGGTTTACG ATGAAGATGT TGGTATTAAC TACAGGGAAG TCACTTTTGT TCCTGGTTTG 
TATAAAATCT TTGATGAAAT TCTAGTTAAT GCTGCTGATA ACAAACAAAG GGACCCAAAA 
ATGTCTTGCA TTAGAGTCAC AATGATGCGA AATAATTTAA TTAGTATATG GAATAATGGA 
AAGGGAATTC CTGTTGTTGA ACACAAAGTG GAGAAAATGT ATGTCCCAGC TCTCATATTT 
GGACAGCTCC TGACCTCCAG TAACTACGAT GATGATGAGA AGAAAGTGAC AGGTGGTCGA 
AATGGCTATG GAGCTAAACT GTGTAACATA TTCAGCACCA AATTTACTGT GGAAACAGCC 
AGTAGGGAAT ACAAGAAAAT GTTCAAACAG ACATGGATGG ATAACATGGG GAGAGCTGGT 
GACATGGAGC TCAAGCCCTT TAGCGGAGAG GATTACACGT GTATTACCTT CCAGCCTGAC 
TTATCTAAGT TTAAAATGCA AAGCCTGGAC AAAGATATTG TCGCACTGAT GGTCAGAAGA 
GCATACGATA TTGCTGGTTC CACCAAAGAT GTAAAAGTCT TTCTTAATGG AAACAGGCTG 
CCAGTCAAAG GGTTCCGTAG TTACGTGGAT ATGTATTTGA AGGATAAGGT TGATGAAACT 
GGGAACGCTC TGAAGGTGGT GCATGAACAA GTAAATCCCC GGTGGGAAGT GTGCTTAACA 
ATGAGCGAGA AAGGCTTTCA GCAGATTAGC TTCGTCAACA GCATCGCTAC TTCTAAGGGT 
GGCAGACATG TCGATTATGT AGCCGATCAG ATTGTGAGTA AACTCGTTGA TGTGGTGAAA 
AAGAAGAACA AGGGAGGTGT TGCAGTGAAG GCCGATCAGG TGAAGAATCA CATGTGGATT 
TTTGGAAATG CGGTAATTGA AAACCCAACC TTTGATTCTC AAACAAAAGA AAACATGACT 
TTACAAGCCA AGAGCTTTGG ATCAACTTGT CAATTAAGTG AAAAATTCAT CAAAGCTGCA 
ATTGGTTGTG GTATTGTCGA AAGCATACTA AACTGGGTGA AGTTTAAGGC CCAAATCCAA 
TTAAACAAGA AGTGTTCAGC TGTAAAACAT AACAGGATCA AGGGAATCCC CAAACTTGAC 
GACGCCAATG ACGCAGGGAG CCGAAACTCT GCTGAATGCA CACTTATCCT GACTGAAGGG 
GACTCAGCCA AAACTCTGGC AGTCTCAGGA CTTGGAGTGG TTGGAAGAGA CAAATATGGG 
GTGTTCCCTC TCAGAGGAAA AATACTCAAT GTGCGAGAAG CTTCTCATAA ACAGATCATG 
GAAAATGCTG AAATTAACAA TATCATCAAG ATTGTGGGGC TTCAGTACAA GAAAAACTAT 
GAGGATGAAG ATTCATTGAA AACTCTTCGT TATGGGAAGA TCATGATTAT GACAGATCAG 
GACCAAGATG GTTCCCACAT CAAAGGCTTG CTGATCAATT TTATCCATCA CAATTGGCCA 
TCTCTTCTGC GACATCGTTT TCTAGAAGAA TTTATCACTC CTATTGTAAA GGTATCTAAA 
AACAAGCAAG AAATAGCATT CTACAGTCTT CCCGAGTTTG AAGAATGGAA GAGTACAAAT 
CCAAATCACA AAAAATGGAA AGTCAAGTAC TACAAAGGTT TGGGCACGAG CACATCAAAG 
GAAGCTAAGG AGTATTTTGC AAATATGAAA CGACATCGTA TTCAGTTCAA ATACTCTGGC 
CCTGAAGACG ATGCTGCGAT CAGCCTGGCC TTTAGTAAAA AACAGGTCGA TGATCGAAAG 
GAATGGTTAA CTAATTTCAT GGAAGATAGA AGACAGCGGA AGTTACTTGG CCTTCCTGAG 
GATTATTTGT ATGGACAAAC CACTATGTAC CTGACCTATA ATGATTTCAT TAACAAGGAA 
CTTATCCTCT TTTCAAACTC TGATAATGAA AGATCTATCC CATCTATGGT AGATGGTTTG 
AAACCAGGTC AGAGAAAGGT TTTGTTTACT TGTTTCAAAC GGAATGACAA GCGAGAGGTG 
AAAGTTGCCC AGTTAGCTGG GTCAGTGGCA GAAATGTCCT CTTATCACCA TGGTGAGATG 
TCGCTGATGA TGACCATTAT CAATTTGGCT CAGAATTTTG TGGGTAGCAA TAATTTGAAC 
CTTCTACAGC CTATTGGTCA GTTTGGTACC AGGCTGCATG GTGGCAAGGA CTCAGCCAGC 
CCTAGGTACA TCTTTACAAT GCTCAGCCCT TTGGCTCGGT TGTTATTTCC ATCAAAAGAT 
GATCACACGT TACGGTTTCT GTATGACGAC AACCAGCGTG TTGAGCCTGA ATGGTACATC 
CCTATTATAC CCATGGTGCT GATAAATGGT GCTGAGGGAA TTGGTACTGG GTGGTCCTGC 
AAAATCCCCA ACTTTGATGT TCGTGAAGTC GTAAATAATA TCAGGCGGCT GTTGGATGGA 
GAAGAGCCTC TGCCCATGCT CCCAAGTTAT AAGAATTACA AAGGTACTAT TGAAGAACTG 
GCTTCAAATC AGTATGTGAT TAATGGAGAA GTAGCTATTC TGAATTCCAC AACCATTGAG 
ATCACTGAGC TTCCCATCAG AACATGGACC CAGACATATA AGGAACAGGT TCTAGAACCT 
ATGTTGAATG GCACCGAGAA GACACCCCCT CTAATAACAG ACTATAGGGA ATACCACACA 
GATACCACTG TGAAGTTTGT CATAAAGATG ACTGAAGAAA AATTGGCAGA GGCAGAGAGA 
GTGGGACTAC ACAAAGTCTT CAAACTCCAG ACTAGTCTCA CTTGCAACTC TATGGTACTT 
TTTGACCATG TTGGTTGTTT AAAGAAATAT GACACTGTGT TGGATATTCT AAGAGACTTC 
TTTGAGCTCA GGCTTAAATA TTATGGATTA AGAAAAGAAT GGCTTCTAGG AATGCTTGGT 
GCAGAATCTT CTAAACTGAA TAATCAAGCT CGCTTTATAT TAGAGAAAAT AGATGGCAAA 
ATAGTCATTG AAAATAAACC TAAGAAAGAA TTAATTAAAG TTCTGATTCA GAGAGGCTAT 
GACTCCGACC CTGTGAAAGC CTGGAAAGAA GCTCAGCAGA AGGTCCCAGA AGAAGAGGAA 
AATGAAGAGA ATGAAGAGAG CGAGAGTGAA AGCACGAGCC CTGCAGCGGA GTCTGGACCG 
ACCTTCAACT ACCTTCTTGA TATGCCCCTG TGGTATCTAA CCAAGGAGAA GAAGGACGAG 
CTGTGCAAGC AAAGGGACGA GAAGGAACAA GAGCTCAACA CGTTAAAGAA AAAGACTCCA 
TCGGATTTGT GGAAGGAAGA CCTGGCTGCT TTTGTGGAAG AACTGGAGGT TGTTGAAGCC 
AAGGAAAAAC AGGATGAACA AGTAGGACTT CCTGGAAAGG GGGTGAAAGC GAAGGGGAAG 
AAAGCGCAGA TATCTGAAGT CCTGCCTTCT CCTGTGGGGA AAAGGGTCAT CCCGCAGGTG 
ACTATGGAGA TGAGAGCTGA GGCAGAGAAG AAAATCAGGA GGAAAATTAA GAGCGAGAAT 
GTTGAGGGCA CCCCTGCTGA GGATGGTGCT GAGCCAGGAC TCCGGCAGAG ACTAGAGAAG 
AGGCAGAAGA GAGAGCCAGG TACCAGGGCA AAGAAGCAAA CTACATTGCC ATTTAAGCCT 
ATCAAAAAAG CCCAAAAGCA AAACCCTTGG TCTGATTCTG AATCAGATAT GAGCAGCAAC 
GAAAGCAATT TTGATGTCCC TCCACGAGAG AAAGAGCCAA GAATTGCTGC AACAAAAGCC 
AAATTCACGG CGGATTTAGA CTCTGATGAC GATTTCTCAG GCTTGGATGA GAAGGATGAG 
GATGAAGATT TCTTCCCATT AGATGATACT CCACCTAAGA CCAAAATGCC CCCAAAAAAT 
ACTAAAAAAG CACTGAAGCC ACAGAAGAGC TCCACATCAG TTGATCTTGA AAGTGATGGG 
AAAGACAGTG TACCAGCTTC TCCAGGCGCT TCTGCTGCTG ACGTCCCAGC TGAGACTGAA 
CCCTCCAAGC CGTCCTCCAA ACAGACAGTG GGAGTGAAGA GGACAATAAC CAAAGGTCAG 
TCTTTAACCT CCACTGCTGG CACCAAAAAG AGAGCTGTAC CAAAGGAGAC CAAGTCAGAT 
TCAGCCTTGA ATGCTCATGT CTCCAAAAAG CCTGCTCCTG CCAAAGCCAA GAACAGTCGC 
AAGAGGATGC CATCTTCTTC TGACAGCTCG GACTCTGAGT TTGAGAAGGC GATTTCTAAA 
GGTGCCACAA GCAAGAAACT CAAGGGAGAG GAGCGGGATT TCCATGTGGA CTTAGATGAC 
ACTGTGGCTC CTCGAGCAAA ATCCGGACGA GCAAGGAAGC CGATTAAGTA CCTGGAGGAG 
TCGGATGACG ACCTCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA-binding activity of rat DNA topoisomerase II ? C-terminal domain contributes to efficient DNA catenation in vitro.
Journal of biochemistry159(3)363-9(2016 Mar)
Kawano S,Kato Y,Okada N,Sano K,Tsutsui K,Tsutsui KM,Ikeda S

Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase II?: A story behind the molecular modeling approach.
Biochemical pharmacology98(1)243-66(2015 Nov)
Mladenovi? M,Stankovi? N,Mati? S,Stani? S,Mihailovi? M,Mihailovi? V,Katani? J,Boroja T,Vukovi? N

Anthracyclines/trastuzumab: new aspects of cardiotoxicity and molecular mechanisms.
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Top2a cDNA ORF clone, Rattus norvegicus(Norway rat)

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