Tph2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tph2
Entrez Gene ID	317675
Full Name	tryptophan hydroxylase 2
Synonyms	Ntph
General protein information	Preferred Names tryptophan hydroxylase 2 Names tryptophan 5-hydroxylase 2 neuronal tryptophan hydroxylase tryptophan 5-monooxygenase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	isoform of tryptophan hydroxylase; may play a role in seratonin biosynthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594904.1	XP_017450393.1	tryptophan 5-hydroxylase 2 isoform X2
NM_173839.2	NP_776211.1	tryptophan 5-hydroxylase 2
XM_008765357.2	XP_008763579.1	tryptophan 5-hydroxylase 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tph2	NP_776211.1
Caenorhabditis elegans (roundworm)	tph-1	NP_495584.4
Homo sapiens (human)	TPH2	NP_775489.2
Canis lupus familiaris (dog)	TPH2	NP_001184049.1
Gallus gallus (chicken)	TPH2	NP_001001301.1
Mus musculus (house mouse)	Tph2	NP_775567.2
Xenopus tropicalis (tropical clawed frog)	tph2	XP_004913008.1
Pan troglodytes (chimpanzee)	TPH2	XP_522470.2
	TPH2	NP_001035035.1
Danio rerio (zebrafish)	tph2	NP_999960.1

Gene Ontology
Bibliography

Related articles in PubMed

Chronic non-invasive corticosterone administration abolishes the diurnal pattern of tph2 expression.
Donner NC, Montoya CD, Lukkes JL, Lowry CA
Psychoneuroendocrinology37(5)645-61(2012 May)

Elevated tph2 mRNA expression in a rat model of chronic anxiety.
Donner NC, Johnson PL, Fitz SD, Kellen KE, Shekhar A, Lowry CA
Depression and anxiety29(4)307-19(2012 Apr)

TPH2 in the ventral tegmental area of the male rat brain.
Carkaci-Salli N, Salli U, Kuntz-Melcavage KL, Pennock MM, Ozgen H, Tekin I, Freeman WM, Vrana KE
Brain research bulletin84(6)376-80(2011 Apr)

Overexpression or knockdown of rat tryptophan hyroxylase-2 has opposing effects on anxiety behavior in an estrogen-dependent manner.
Hiroi R, McDevitt RA, Morcos PA, Clark MS, Neumaier JF
Neuroscience176120-31(2011 Mar)

Postnatal changes in tryptophan hydroxylase and serotonin transporter immunoreactivity in multiple brainstem nuclei of the rat: implications for a sensitive period.
Liu Q, Wong-Riley MT
The Journal of comparative neurology518(7)1082-97(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data demonstrated that chronic elevation of corticosterone creates a vulnerability to a depression-like syndrome that is associated with increased tph2 expression, similar to that observed in depressed patients.
Title: Chronic non-invasive corticosterone administration abolishes the diurnal pattern of tph2 expression.

Chronic anxiety states are associated with dysregulated tph2 expression.
Title: Elevated tph2 mRNA expression in a rat model of chronic anxiety.

The results of this study indicate that TPH2 and serotonergic signaling may play an important role in the mesolimbic/mesocortical reward pathway.
Title: TPH2 in the ventral tegmental area of the male rat brain.

estrogen and TpH2 in the caudal dorsal raphe nucleus have a critical interaction in regulating anxiety-like behavior.
Title: Overexpression or knockdown of rat tryptophan hyroxylase-2 has opposing effects on anxiety behavior in an estrogen-dependent manner.

Data showed that multiple brainstem nuclei exhibited a significant decline in tryptophan hydroxylase immunoreactivity during postnatal days 2-21.
Title: Postnatal changes in tryptophan hydroxylase and serotonin transporter immunoreactivity in multiple brainstem nuclei of the rat: implications for a sensitive period.

The following Tph2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tph2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03776	NM_173839.2 Latest version!	Rattus norvegicus tryptophan hydroxylase 2 (Tph2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa47638	XM_008765357.2 Latest version!	Rattus norvegicus tryptophan hydroxylase 2 (Tph2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa62029	XM_017594904.1 Latest version!	Rattus norvegicus tryptophan hydroxylase 2 (Tph2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tph2 ( NM_173839.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_776211.1 Rattus norvegicus tryptophan hydroxylase 2 (Tph2), mRNA.

Price & Availability↑

CloneID	ORa03776
Clone ID Related Accession (Same CDS sequence)	NM_173839.2
Accession Version	NM_173839.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	tryptophan 5-hydroxylase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY098915.1. On Jun 3, 2003 this sequence version replaced NM_173839.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY098915.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCCCG CAATGATGAT GTTTTCCAGT AAATACTGGG CCAGGAGAGG GTTGTCCTTG 
GATTCAGCGG TGCCAGAAGA GCATCAGATA CTTGGCGGCT TAACACAAAA TAAGGCTACC 
GCTAGCAAAA GCGAGGACAA GAGAAGCGGC AAAGACACTT CGGAGAGCAG CAAGACTGCG 
GTTGTGTTCT CCCTGAAGAA TGAAGTTGGC GGGCTGGTGA GAGCACTGAG ACTCTTCCAG 
GAAAAACACG TCAACATGCT CCATATTGAA TCCAGGAGGT CCCGGCGAAG AAGTTCTGAA 
GTCGAAATCT TCGTGGACTG TGAATGTGGC AAAACAGAAT TCAACGAGCT CATTCAGTTG 
CTCAAGTTTC AGACCACCAT TGTGACGCTG AATCCACCTG ACAACATTTG GACGGAGGAA 
GAAGAACTAG AGGATGTGCC GTGGTTCCCT CGGAAGATCT CTGAGTTAGA CAGATGCTCT 
CACAGAGTCC TCATGTACGG CACCGAGCTT GACGCCGACC ACCCAGGATT CAAGGACAAC 
GTCTATCGAC AGAGGAGGAA GTATTTTGTG GATGTGGCCA TGGGTTATAA ATATGGCCAG 
CCCATTCCCA GGGTGGAATA CACAGAAGAA GAGACTAAAA CTTGGGGTGT TGTGTTTCGG 
GAGCTCTCCA AACTCTACCC CACTCATGCT TGCCGAGAGT ACCTGAAAAA CTTCCCCCTG 
CTGACCAAGT ACTGCGGCTA CAGGGAAGAC AACGTCCCGC AGCTGGAAGA CGTCTCCATG 
TTTCTGAAAG AGCGATCTGG CTTCACAGTG AGACCAGTGG CTGGCTACCT GAGCCCAAGA 
GACTTCCTGG CTGGGCTGGC CTACAGAGTA TTCCACTGCA CTCAGTACGT GCGGCATGGC 
TCCGACCCCC TCTACACCCC GGAACCAGAC ACATGCCATG AGCTCCTGGG ACATGTGCCA 
CTGCTGGCGG ATCCCAAGTT CGCTCAGTTT TCTCAAGAAA TAGGCTTAGC CTCTCTGGGA 
GCCTCAGATG AAGACGTTCA GAAACTGGCC ACGTGCTATT TCTTCACAAT CGAGTTCGGC 
CTTTGCAAGC AAGAAGGTCA ACTGCGGGCG TACGGAGCAG GGTTACTTTC CTCCATCGGA 
GAATTGAAGC ATGCTCTTTC TGACAAGGCG TGTGTAAAAG CCTTTGACCC GAAGACAACC 
TGCTTGCAGG AATGCCTAAT CACCACCTTC CAAGATGCTT ACTTTGTTTC TGAAAGTTTT 
GAAGAAGCCA AAGAGAAGAT GAGGGATTTT GCAAAGTCAA TTACCCGTCC TTTCTCAGTA 
TATTTCAACC CCTACACACA GAGCATTGAA ATTCTGAAAG ACACCAGAAG TATTGAGAAT 
GTGGTGCAGG ACCTGCGCAG TGATTTGAAC ACCGTGTGCG ACGCCTTGAA TAAAATGAAC 
CAATATTTGG GGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776211.1
CDS	118..1575
Translation	MQPAMMMFSSKYWARRGLSLDSAVPEEHQILGGLTQNKATASKSEDKRSGKDTSESSKTAVVFSLKNEVGGLVRALRLFQEKHVNMLHIESRRSRRRSSEVEIFVDCECGKTEFNELIQLLKFQTTIVTLNPPDNIWTEEEELEDVPWFPRKISELDRCSHRVLMYGTELDADHPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLKERSGFTVRPVAGYLSPRDFLAGLAYRVFHCTQYVRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQDAYFVSESFEEAKEKMRDFAKSITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDLNTVCDALNKMNQYLGI

Target ORF information:

RefSeq Version	NM_173839.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tryptophan hydroxylase 2 (Tph2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173839.2

ORF Insert Sequence:

ATGCAGCCCG CAATGATGAT GTTTTCCAGT AAATACTGGG CCAGGAGAGG GTTGTCCTTG 
GATTCAGCGG TGCCAGAAGA GCATCAGATA CTTGGCGGCT TAACACAAAA TAAGGCTACC 
GCTAGCAAAA GCGAGGACAA GAGAAGCGGC AAAGACACTT CGGAGAGCAG CAAGACTGCG 
GTTGTGTTCT CCCTGAAGAA TGAAGTTGGC GGGCTGGTGA GAGCACTGAG ACTCTTCCAG 
GAAAAACACG TCAACATGCT CCATATTGAA TCCAGGAGGT CCCGGCGAAG AAGTTCTGAA 
GTCGAAATCT TCGTGGACTG TGAATGTGGC AAAACAGAAT TCAACGAGCT CATTCAGTTG 
CTCAAGTTTC AGACCACCAT TGTGACGCTG AATCCACCTG ACAACATTTG GACGGAGGAA 
GAAGAACTAG AGGATGTGCC GTGGTTCCCT CGGAAGATCT CTGAGTTAGA CAGATGCTCT 
CACAGAGTCC TCATGTACGG CACCGAGCTT GACGCCGACC ACCCAGGATT CAAGGACAAC 
GTCTATCGAC AGAGGAGGAA GTATTTTGTG GATGTGGCCA TGGGTTATAA ATATGGCCAG 
CCCATTCCCA GGGTGGAATA CACAGAAGAA GAGACTAAAA CTTGGGGTGT TGTGTTTCGG 
GAGCTCTCCA AACTCTACCC CACTCATGCT TGCCGAGAGT ACCTGAAAAA CTTCCCCCTG 
CTGACCAAGT ACTGCGGCTA CAGGGAAGAC AACGTCCCGC AGCTGGAAGA CGTCTCCATG 
TTTCTGAAAG AGCGATCTGG CTTCACAGTG AGACCAGTGG CTGGCTACCT GAGCCCAAGA 
GACTTCCTGG CTGGGCTGGC CTACAGAGTA TTCCACTGCA CTCAGTACGT GCGGCATGGC 
TCCGACCCCC TCTACACCCC GGAACCAGAC ACATGCCATG AGCTCCTGGG ACATGTGCCA 
CTGCTGGCGG ATCCCAAGTT CGCTCAGTTT TCTCAAGAAA TAGGCTTAGC CTCTCTGGGA 
GCCTCAGATG AAGACGTTCA GAAACTGGCC ACGTGCTATT TCTTCACAAT CGAGTTCGGC 
CTTTGCAAGC AAGAAGGTCA ACTGCGGGCG TACGGAGCAG GGTTACTTTC CTCCATCGGA 
GAATTGAAGC ATGCTCTTTC TGACAAGGCG TGTGTAAAAG CCTTTGACCC GAAGACAACC 
TGCTTGCAGG AATGCCTAAT CACCACCTTC CAAGATGCTT ACTTTGTTTC TGAAAGTTTT 
GAAGAAGCCA AAGAGAAGAT GAGGGATTTT GCAAAGTCAA TTACCCGTCC TTTCTCAGTA 
TATTTCAACC CCTACACACA GAGCATTGAA ATTCTGAAAG ACACCAGAAG TATTGAGAAT 
GTGGTGCAGG ACCTGCGCAG TGATTTGAAC ACCGTGTGCG ACGCCTTGAA TAAAATGAAC 
CAATATTTGG GGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tph2 ( XM_008765357.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763579.1 Rattus norvegicus tryptophan hydroxylase 2 (Tph2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47638
Clone ID Related Accession (Same CDS sequence)	XM_008765357.2
Accession Version	XM_008765357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tryptophan 5-hydroxylase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCG CAATGATGAT GTTTTCCAGT AAATACTGGG CCAGGAGAGG GTTGTCCTTG 
GATTCAGCGG TGCCAGAAGA GCATCAGATA CTTGGCGGCT TAACACAAAA TAAGGCTACC 
GCTAGCAAAA GCGAGGACAA GAGAAGCGGC AAAGACACTT CGGAGAGCAG CAAGACTGCG 
GTTGTGTTCT CCCTGAAGAA TGAAGTTGGC GGGCTGGTGA GAGCACTGAG ACTCTTCCAG 
GAAAAACACG TCAACATGCT CCATATTGAA TCCAGGAGGT CCCGGCGAAG AAGTTCTGAA 
GTCGAAATCT TCGTGGACTG TGAATGTGGC AAAACAGAAT TCAACGAGCT CATTCAGTTG 
CTCAAGTTTC AGACCACCAT TGTGACGCTG AATCCACCTG ACAACATTTG GACGGAGGAA 
GAAGGCAAGG CAGAACTAGA GGATGTGCCG TGGTTCCCTC GGAAGATCTC TGAGTTAGAC 
AGATGCTCTC ACAGAGTCCT CATGTACGGC ACCGAGCTTG ACGCCGACCA CCCAGGATTC 
AAGGACAACG TCTATCGACA GAGGAGGAAG TATTTTGTGG ATGTGGCCAT GGGTTATAAA 
TATGGCCAGC CCATTCCCAG GGTGGAATAC ACAGAAGAAG AGACTAAAAC TTGGGGTGTT 
GTGTTTCGGG AGCTCTCCAA ACTCTACCCC ACTCATGCTT GCCGAGAGTA CCTGAAAAAC 
TTCCCCCTGC TGACCAAGTA CTGCGGCTAC AGGGAAGACA ACGTCCCGCA GCTGGAAGAC 
GTCTCCATGT TTCTGAAAGA GCGATCTGGC TTCACAGTGA GACCAGTGGC TGGCTACCTG 
AGCCCAAGAG ACTTCCTGGC TGGGCTGGCC TACAGAGTAT TCCACTGCAC TCAGTACGTG 
CGGCATGGCT CCGACCCCCT CTACACCCCG GAACCAGACA CATGCCATGA GCTCCTGGGA 
CATGTGCCAC TGCTGGCGGA TCCCAAGTTC GCTCAGTTTT CTCAAGAAAT AGGCTTAGCC 
TCTCTGGGAG CCTCAGATGA AGACGTTCAG AAACTGGCCA CGTGCTATTT CTTCACAATC 
GAGTTCGGCC TTTGCAAGCA AGAAGGTCAA CTGCGGGCGT ACGGAGCAGG GTTACTTTCC 
TCCATCGGAG AATTGAAGCA TGCTCTTTCT GACAAGGCGT GTGTAAAAGC CTTTGACCCG 
AAGACAACCT GCTTGCAGGA ATGCCTAATC ACCACCTTCC AAGATGCTTA CTTTGTTTCT 
GAAAGTTTTG AAGAAGCCAA AGAGAAGATG AGGGATTTTG CAAAGTCAAT TACCCGTCCT 
TTCTCAGTAT ATTTCAACCC CTACACACAG AGCATTGAAA TTCTGAAAGA CACCAGAAGT 
ATTGAGAATG TGGTGCAGGA CCTGCGCAGT GATTTGAACA CCGTGTGCGA CGCCTTGAAT 
AAAATGAACC AATATTTGGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763579.1
CDS	275..1741
Translation	MQPAMMMFSSKYWARRGLSLDSAVPEEHQILGGLTQNKATASKSEDKRSGKDTSESSKTAVVFSLKNEVGGLVRALRLFQEKHVNMLHIESRRSRRRSSEVEIFVDCECGKTEFNELIQLLKFQTTIVTLNPPDNIWTEEEGKAELEDVPWFPRKISELDRCSHRVLMYGTELDADHPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLKERSGFTVRPVAGYLSPRDFLAGLAYRVFHCTQYVRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQDAYFVSESFEEAKEKMRDFAKSITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDLNTVCDALNKMNQYLGI

Target ORF information:

RefSeq Version	XM_008765357.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tryptophan hydroxylase 2 (Tph2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765357.2

ORF Insert Sequence:

ATGCAGCCCG CAATGATGAT GTTTTCCAGT AAATACTGGG CCAGGAGAGG GTTGTCCTTG 
GATTCAGCGG TGCCAGAAGA GCATCAGATA CTTGGCGGCT TAACACAAAA TAAGGCTACC 
GCTAGCAAAA GCGAGGACAA GAGAAGCGGC AAAGACACTT CGGAGAGCAG CAAGACTGCG 
GTTGTGTTCT CCCTGAAGAA TGAAGTTGGC GGGCTGGTGA GAGCACTGAG ACTCTTCCAG 
GAAAAACACG TCAACATGCT CCATATTGAA TCCAGGAGGT CCCGGCGAAG AAGTTCTGAA 
GTCGAAATCT TCGTGGACTG TGAATGTGGC AAAACAGAAT TCAACGAGCT CATTCAGTTG 
CTCAAGTTTC AGACCACCAT TGTGACGCTG AATCCACCTG ACAACATTTG GACGGAGGAA 
GAAGGCAAGG CAGAACTAGA GGATGTGCCG TGGTTCCCTC GGAAGATCTC TGAGTTAGAC 
AGATGCTCTC ACAGAGTCCT CATGTACGGC ACCGAGCTTG ACGCCGACCA CCCAGGATTC 
AAGGACAACG TCTATCGACA GAGGAGGAAG TATTTTGTGG ATGTGGCCAT GGGTTATAAA 
TATGGCCAGC CCATTCCCAG GGTGGAATAC ACAGAAGAAG AGACTAAAAC TTGGGGTGTT 
GTGTTTCGGG AGCTCTCCAA ACTCTACCCC ACTCATGCTT GCCGAGAGTA CCTGAAAAAC 
TTCCCCCTGC TGACCAAGTA CTGCGGCTAC AGGGAAGACA ACGTCCCGCA GCTGGAAGAC 
GTCTCCATGT TTCTGAAAGA GCGATCTGGC TTCACAGTGA GACCAGTGGC TGGCTACCTG 
AGCCCAAGAG ACTTCCTGGC TGGGCTGGCC TACAGAGTAT TCCACTGCAC TCAGTACGTG 
CGGCATGGCT CCGACCCCCT CTACACCCCG GAACCAGACA CATGCCATGA GCTCCTGGGA 
CATGTGCCAC TGCTGGCGGA TCCCAAGTTC GCTCAGTTTT CTCAAGAAAT AGGCTTAGCC 
TCTCTGGGAG CCTCAGATGA AGACGTTCAG AAACTGGCCA CGTGCTATTT CTTCACAATC 
GAGTTCGGCC TTTGCAAGCA AGAAGGTCAA CTGCGGGCGT ACGGAGCAGG GTTACTTTCC 
TCCATCGGAG AATTGAAGCA TGCTCTTTCT GACAAGGCGT GTGTAAAAGC CTTTGACCCG 
AAGACAACCT GCTTGCAGGA ATGCCTAATC ACCACCTTCC AAGATGCTTA CTTTGTTTCT 
GAAAGTTTTG AAGAAGCCAA AGAGAAGATG AGGGATTTTG CAAAGTCAAT TACCCGTCCT 
TTCTCAGTAT ATTTCAACCC CTACACACAG AGCATTGAAA TTCTGAAAGA CACCAGAAGT 
ATTGAGAATG TGGTGCAGGA CCTGCGCAGT GATTTGAACA CCGTGTGCGA CGCCTTGAAT 
AAAATGAACC AATATTTGGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tph2 ( XM_017594904.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450393.1 Rattus norvegicus tryptophan hydroxylase 2 (Tph2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62029
Clone ID Related Accession (Same CDS sequence)	XM_017594904.1
Accession Version	XM_017594904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tryptophan 5-hydroxylase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCG CAATGATGAT GTTTTCCAGT AAATACTGGG CCAGGAGAGG GTTGTCCTTG 
GATTCAGCGG TGCCAGAAGA GCATCAGATA CTTGGCGGCT TAACAGTGAG TATGGATGAC 
TCCTGTGCTC CTACTCTCTT GAAGCAAAAT AAGGCTACCG CTAGCAAAAG CGAGGACAAG 
AGAAGCGGCA AAGACACTTC GGAGAGCAGC AAGACTGCGG TTGTGTTCTC CCTGAAGAAT 
GAAGTTGGCG GGCTGGTGAG AGCACTGAGA CTCTTCCAGG AAAAACACGT CAACATGCTC 
CATATTGAAT CCAGGAGGTC CCGGCGAAGA AGTTCTGAAG TCGAAATCTT CGTGGACTGT 
GAATGTGGCA AAACAGAATT CAACGAGCTC ATTCAGTTGC TCAAGTTTCA GACCACCATT 
GTGACGCTGA ATCCACCTGA CAACATTTGG ACGGAGGAAG AAGAACTAGA GGATGTGCCG 
TGGTTCCCTC GGAAGATCTC TGAGTTAGAC AGATGCTCTC ACAGAGTCCT CATGTACGGC 
ACCGAGCTTG ACGCCGACCA CCCAGGATTC AAGGACAACG TCTATCGACA GAGGAGGAAG 
TATTTTGTGG ATGTGGCCAT GGGTTATAAA TATGGCCAGC CCATTCCCAG GGTGGAATAC 
ACAGAAGAAG AGACTAAAAC TTGGGGTGTT GTGTTTCGGG AGCTCTCCAA ACTCTACCCC 
ACTCATGCTT GCCGAGAGTA CCTGAAAAAC TTCCCCCTGC TGACCAAGTA CTGCGGCTAC 
AGGGAAGACA ACGTCCCGCA GCTGGAAGAC GTCTCCATGT TTCTGAAAGA GCGATCTGGC 
TTCACAGTGA GACCAGTGGC TGGCTACCTG AGCCCAAGAG ACTTCCTGGC TGGGCTGGCC 
TACAGAGTAT TCCACTGCAC TCAGTACGTG CGGCATGGCT CCGACCCCCT CTACACCCCG 
GAACCAGACA CATGCCATGA GCTCCTGGGA CATGTGCCAC TGCTGGCGGA TCCCAAGTTC 
GCTCAGTTTT CTCAAGAAAT AGGCTTAGCC TCTCTGGGAG CCTCAGATGA AGACGTTCAG 
AAACTGGCCA CGTGCTATTT CTTCACAATC GAGTTCGGCC TTTGCAAGCA AGAAGGTCAA 
CTGCGGGCGT ACGGAGCAGG GTTACTTTCC TCCATCGGAG AATTGAAGCA TGCTCTTTCT 
GACAAGGCGT GTGTAAAAGC CTTTGACCCG AAGACAACCT GCTTGCAGGA ATGCCTAATC 
ACCACCTTCC AAGATGCTTA CTTTGTTTCT GAAAGTTTTG AAGAAGCCAA AGAGAAGATG 
AGGGATTTTG CAAAGTCAAT TACCCGTCCT TTCTCAGTAT ATTTCAACCC CTACACACAG 
AGCATTGAAA TTCTGAAAGA CACCAGAAGT ATTGAGAATG TGGTGCAGGA CCTGCGCAGT 
GATTTGAACA CCGTGTGCGA CGCCTTGAAT AAAATGAACC AATATTTGGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450393.1
CDS	277..1773
Translation	MQPAMMMFSSKYWARRGLSLDSAVPEEHQILGGLTVSMDDSCAPTLLKQNKATASKSEDKRSGKDTSESSKTAVVFSLKNEVGGLVRALRLFQEKHVNMLHIESRRSRRRSSEVEIFVDCECGKTEFNELIQLLKFQTTIVTLNPPDNIWTEEEELEDVPWFPRKISELDRCSHRVLMYGTELDADHPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLKERSGFTVRPVAGYLSPRDFLAGLAYRVFHCTQYVRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQDAYFVSESFEEAKEKMRDFAKSITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDLNTVCDALNKMNQYLGI

Target ORF information:

RefSeq Version	XM_017594904.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tryptophan hydroxylase 2 (Tph2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594904.1

ORF Insert Sequence:

ATGCAGCCCG CAATGATGAT GTTTTCCAGT AAATACTGGG CCAGGAGAGG GTTGTCCTTG 
GATTCAGCGG TGCCAGAAGA GCATCAGATA CTTGGCGGCT TAACAGTGAG TATGGATGAC 
TCCTGTGCTC CTACTCTCTT GAAGCAAAAT AAGGCTACCG CTAGCAAAAG CGAGGACAAG 
AGAAGCGGCA AAGACACTTC GGAGAGCAGC AAGACTGCGG TTGTGTTCTC CCTGAAGAAT 
GAAGTTGGCG GGCTGGTGAG AGCACTGAGA CTCTTCCAGG AAAAACACGT CAACATGCTC 
CATATTGAAT CCAGGAGGTC CCGGCGAAGA AGTTCTGAAG TCGAAATCTT CGTGGACTGT 
GAATGTGGCA AAACAGAATT CAACGAGCTC ATTCAGTTGC TCAAGTTTCA GACCACCATT 
GTGACGCTGA ATCCACCTGA CAACATTTGG ACGGAGGAAG AAGAACTAGA GGATGTGCCG 
TGGTTCCCTC GGAAGATCTC TGAGTTAGAC AGATGCTCTC ACAGAGTCCT CATGTACGGC 
ACCGAGCTTG ACGCCGACCA CCCAGGATTC AAGGACAACG TCTATCGACA GAGGAGGAAG 
TATTTTGTGG ATGTGGCCAT GGGTTATAAA TATGGCCAGC CCATTCCCAG GGTGGAATAC 
ACAGAAGAAG AGACTAAAAC TTGGGGTGTT GTGTTTCGGG AGCTCTCCAA ACTCTACCCC 
ACTCATGCTT GCCGAGAGTA CCTGAAAAAC TTCCCCCTGC TGACCAAGTA CTGCGGCTAC 
AGGGAAGACA ACGTCCCGCA GCTGGAAGAC GTCTCCATGT TTCTGAAAGA GCGATCTGGC 
TTCACAGTGA GACCAGTGGC TGGCTACCTG AGCCCAAGAG ACTTCCTGGC TGGGCTGGCC 
TACAGAGTAT TCCACTGCAC TCAGTACGTG CGGCATGGCT CCGACCCCCT CTACACCCCG 
GAACCAGACA CATGCCATGA GCTCCTGGGA CATGTGCCAC TGCTGGCGGA TCCCAAGTTC 
GCTCAGTTTT CTCAAGAAAT AGGCTTAGCC TCTCTGGGAG CCTCAGATGA AGACGTTCAG 
AAACTGGCCA CGTGCTATTT CTTCACAATC GAGTTCGGCC TTTGCAAGCA AGAAGGTCAA 
CTGCGGGCGT ACGGAGCAGG GTTACTTTCC TCCATCGGAG AATTGAAGCA TGCTCTTTCT 
GACAAGGCGT GTGTAAAAGC CTTTGACCCG AAGACAACCT GCTTGCAGGA ATGCCTAATC 
ACCACCTTCC AAGATGCTTA CTTTGTTTCT GAAAGTTTTG AAGAAGCCAA AGAGAAGATG 
AGGGATTTTG CAAAGTCAAT TACCCGTCCT TTCTCAGTAT ATTTCAACCC CTACACACAG 
AGCATTGAAA TTCTGAAAGA CACCAGAAGT ATTGAGAATG TGGTGCAGGA CCTGCGCAGT 
GATTTGAACA CCGTGTGCGA CGCCTTGAAT AAAATGAACC AATATTTGGG GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chronic non-invasive corticosterone administration abolishes the diurnal pattern of tph2 expression.
Psychoneuroendocrinology37(5)645-61(2012 May)
Donner NC,Montoya CD,Lukkes JL,Lowry CA

Elevated tph2 mRNA expression in a rat model of chronic anxiety.
Depression and anxiety29(4)307-19(2012 Apr)
Donner NC,Johnson PL,Fitz SD,Kellen KE,Shekhar A,Lowry CA

TPH2 in the ventral tegmental area of the male rat brain.
Brain research bulletin84(6)376-80(2011 Apr)
Carkaci-Salli N,Salli U,Kuntz-Melcavage KL,Pennock MM,Ozgen H,Tekin I,Freeman WM,Vrana KE

Overexpression or knockdown of rat tryptophan hyroxylase-2 has opposing effects on anxiety behavior in an estrogen-dependent manner.
Neuroscience176120-31(2011 Mar)
Hiroi R,McDevitt RA,Morcos PA,Clark MS,Neumaier JF

Postnatal changes in tryptophan hydroxylase and serotonin transporter immunoreactivity in multiple brainstem nuclei of the rat: implications for a sensitive period.
The Journal of comparative neurology518(7)1082-97(2010 Apr)
Liu Q,Wong-Riley MT