Ddx3x cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx3x
Entrez Gene ID	317335
Full Name	DEAD-box helicase 3, X-linked
General protein information	Preferred Names DEAD-box helicase 3, X-linked Names ATP-dependent RNA helicase DDX3X DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256653.3	XP_006256715.1	ATP-dependent RNA helicase DDX3X isoform X1
XM_006256652.3	XP_006256714.1	ATP-dependent RNA helicase DDX3X isoform X2
NM_001108246.1	NP_001101716.1	ATP-dependent RNA helicase DDX3X

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RNA Remodeling Activity of DEAD Box Proteins Tuned by Protein Concentration, RNA Length, and ATP.
Kim Y, Myong S
Molecular cell63(5)865-76(2016 Sep)

The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
Zhang Y, You J, Wang X, Weber J
Molecular and cellular biology35(17)2918-31(2015 Sep)

Mutations in DDX3X Are a Common Cause of Unexplained Intellectual Disability with Gender-Specific Effects on Wnt Signaling.
Snijders Blok L, Madsen E, Juusola J, Gilissen C, Baralle D, Reijnders MR, Venselaar H, Helsmoortel C, Cho MT, Hoischen A, Vissers LE, Koemans TS, Wissink-Lindhout W, Eichler EE, Romano C, Van Esch H, Stumpel C, Vreeburg M, Smeets E, Oberndorff K, van Bon BW, Shaw M, Gecz J, Haan E, Bienek M, Jensen C, Loeys BL, Van Dijck A, Innes AM, Racher H, Vermeer S, Di Donato N, Rump A, Tatton-Brown K, Parker MJ, Henderson A, Lynch SA, Fryer A, Ross A, Vasudevan P, Kini U, Newbury-Ecob R, Chandler K, Male A, , Dijkstra S, Schieving J, Giltay J, van Gassen KL, Schuurs-Hoeijmakers J, Tan PL, Pediaditakis I, Haas SA, Retterer K, Reed P, Monaghan KG, Haverfield E, Natowicz M, Myers A, Kruer MC, Stein Q, Strauss KA, Brigatti KW, Keating K, Burton BK, Kim KH, Charrow J, Norman J, Foster-Barber A, Kline AD, Kimball A, Zackai E, Harr M, Fox J, McLaughlin J, Lindstrom K, Haude KM, van Roozendaal K, Brunner H, Chung WK, Kooy RF, Pfundt R, Kalscheuer V, Mehta SG, Katsanis N, Kleefstra T
American journal of human genetics97(2)343-52(2015 Aug)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP
Journal of virology88(18)10340-53(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx3x gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx3x cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11676	NM_001108246.1 Latest version!	Rattus norvegicus DEAD-box helicase 3, X-linked (Ddx3x), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa39571	XM_006256652.3 Latest version!	Rattus norvegicus DEAD-box helicase 3, X-linked (Ddx3x), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa39572	XM_006256653.3 Latest version!	Rattus norvegicus DEAD-box helicase 3, X-linked (Ddx3x), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx3x ( NM_001108246.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101716.1 Rattus norvegicus DEAD-box helicase 3, X-linked (Ddx3x), mRNA.

Price & Availability↑

CloneID	ORa11676
Clone ID Related Accession (Same CDS sequence)	NM_001108246.1
Accession Version	NM_001108246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent RNA helicase DDX3X
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474009.2. On or before Oct 4, 2007 this sequence version replaced XM_228701.4, XM_001060162.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: FQ211849.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTTAGATA TGGGGTTTGA ACCTCAGATA CGTAGAATAG TTGAACAAGA CACTATGCCT 
CCAAAAGGTG TCCGCCACAC TATGATGTTT AGTGCTACTT TTCCTAAGGA AATACAGATG 
CTGGCCCGTG ATTTCTTAGA TGAGTACATA TTTCTGGCTG TAGGAAGAGT TGGGTCTACT 
TCAGAGAACA TCACACAAAA AGTGGTTTGG GTGGAGGAGA TAGACAAAAG ATCATTTCTG 
CTTGACCTCC TAAATGCAAC AGGCAAGGAT TCCCTGACTC TAGTGTTTGT GGAGACCAAA 
AAGGGTGCAG ATTCGCTGGA GGATTTCTTA TACCATGAAG GATATGCCTG TACCAGTATC 
CATGGAGACC GTTCTCAGAG AGATAGGGAA GAGGCCCTTC ACCAGTTCCG CTCAGGAAAA 
AGCCCAATTC TAGTAGCTAC AGCAGTAGCA GCAAGAGGAC TGGATATTTC AAATGTGAAG 
CATGTTATTA ATTTTGACCT GCCTAGTGAT ATCGAAGAAT ATGTGCATCG CATAGGCCGT 
ACAGGGCGTG TAGGAAACCT TGGTCTTGCC ACCTCATTCT TTAATGAAAG AAATATAAAT 
ATTACTAAGG ATTTACTGGA TCTTCTTGTT GAAGCAAAAC AAGAAGTGCC ATCTTGGTTG 
GAAAACATGG CTTTTGAACA CCACTATAAG GGTAGCAGCC GTGGACGTTC TAAGAGCAGT 
CGATTTAGTG GAGGGTTTGG TGCCAGAGAC TATCGACAGA GTAGCGGCGC CAGCAGTTCC 
AGCTTCAGCA GCAGCCGCGC AAGCAGCAGC CGGAGTGGTG GAGGTGGCCA TGGCAGCAGC 
AGAGGATTTG GTGGAGGTGG CTATGGAGGC TTTTACAACA GTGATGGATA CGGAGGAAAT 
TACAACTCCC AGGGGGTTGA CTGGTGGGGA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101716.1
CDS	870..1805
Translation	MLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEEIDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAFEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN

Target ORF information:

RefSeq Version	NM_001108246.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 3, X-linked (Ddx3x), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108246.1

ORF Insert Sequence:

ATGTTAGATA TGGGGTTTGA ACCTCAGATA CGTAGAATAG TTGAACAAGA CACTATGCCT 
CCAAAAGGTG TCCGCCACAC TATGATGTTT AGTGCTACTT TTCCTAAGGA AATACAGATG 
CTGGCCCGTG ATTTCTTAGA TGAGTACATA TTTCTGGCTG TAGGAAGAGT TGGGTCTACT 
TCAGAGAACA TCACACAAAA AGTGGTTTGG GTGGAGGAGA TAGACAAAAG ATCATTTCTG 
CTTGACCTCC TAAATGCAAC AGGCAAGGAT TCCCTGACTC TAGTGTTTGT GGAGACCAAA 
AAGGGTGCAG ATTCGCTGGA GGATTTCTTA TACCATGAAG GATATGCCTG TACCAGTATC 
CATGGAGACC GTTCTCAGAG AGATAGGGAA GAGGCCCTTC ACCAGTTCCG CTCAGGAAAA 
AGCCCAATTC TAGTAGCTAC AGCAGTAGCA GCAAGAGGAC TGGATATTTC AAATGTGAAG 
CATGTTATTA ATTTTGACCT GCCTAGTGAT ATCGAAGAAT ATGTGCATCG CATAGGCCGT 
ACAGGGCGTG TAGGAAACCT TGGTCTTGCC ACCTCATTCT TTAATGAAAG AAATATAAAT 
ATTACTAAGG ATTTACTGGA TCTTCTTGTT GAAGCAAAAC AAGAAGTGCC ATCTTGGTTG 
GAAAACATGG CTTTTGAACA CCACTATAAG GGTAGCAGCC GTGGACGTTC TAAGAGCAGT 
CGATTTAGTG GAGGGTTTGG TGCCAGAGAC TATCGACAGA GTAGCGGCGC CAGCAGTTCC 
AGCTTCAGCA GCAGCCGCGC AAGCAGCAGC CGGAGTGGTG GAGGTGGCCA TGGCAGCAGC 
AGAGGATTTG GTGGAGGTGG CTATGGAGGC TTTTACAACA GTGATGGATA CGGAGGAAAT 
TACAACTCCC AGGGGGTTGA CTGGTGGGGA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx3x ( XM_006256652.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256714.1 Rattus norvegicus DEAD-box helicase 3, X-linked (Ddx3x), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa39571
Clone ID Related Accession (Same CDS sequence)	XM_006256652.3
Accession Version	XM_006256652.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent RNA helicase DDX3X isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCATG TGGCAGTGGA AAATGCGCTC GGGCTGGACC AGCAGTTTGC TGGCCTAGAC 
CTGAACTCTT CAGATAATCA GACTGGAGGA AGTACAGCAA GCAAAGGGCG CTATATCCCT 
CCTCATTTAA GGAACAGAGA AGCTACTAAA GGATTCTATG ACAAAGACAG TTCAGGGTGG 
AGTTCTAGTA AAGATAAAGA TGCATACAGC AGCTTTGGAT CCCGGGGTGA CTCACGAGGG 
AAGTCTAGCT TCTTTGGTGA TCGTGGAAGT GGATCAAGGG GAAGGTTTGA TGATCGTGGA 
CGGGGAGACT ATGATGGCAT TGGTGGCCGT GGAGATAGGA GTGGCTTTGG CAAATTTGAA 
AGAGGTGGAA ATAGTCGCTG GTGTGATAAA TCAGATGAAG ATGACTGGTC AAAGCCACTC 
CCACCAAGTG AACGCTTGGA ACAGGAACTC TTTTCTGGAG GCAATACTGG GATTAACTTT 
GAGAAATATG ATGACATTCC AGTTGAAGCA ACAGGCAACA ACTGTCCTCC ACATATTGAA 
AGTTTCAGTG ATGTTGAGAT GGGAGAAATT ATTATGGGAA ACATTGAGCT TACTCGTTAT 
ACTCGTCCAA CTCCAGTGCA GAAGCATGCT ATTCCTATTA TCAAAGAGAA AAGAGACTTG 
ATGGCTTGTG CTCAAACAGG CTCTGGAAAA ACTGCAGCAT TTCTCTTGCC CATCTTGAGT 
CAGATTTATG CTGATGGTCC CGGAGAGGCT CTGAGGGCCA TGAAGGAAAA TGGAAGATAT 
GGGCGTCGTA AACAGTATCC AATCTCCTTG GTACTGGCAC CAACGAGAGA ATTGGCAGTG 
CAGATCTATG AGGAAGCCAG AAAATTCTCA TACCGGTCTA GAGTCCGTCC TTGCGTGGTT 
TATGGTGGTG CTGAAATTGG TCAGCAGATT CGAGACTTAG AACGTGGATG CCACTTGTTA 
GTAGCCACTC CAGGACGTCT AGTGGACATG ATGGAGAGAG GGAAGATCGG GCTAGACTTC 
TGCAAATACC TGGTGTTAGA TGAAGCTGAC CGGATGTTAG ATATGGGGTT TGAACCTCAG 
ATACGTAGAA TAGTTGAACA AGACACTATG CCTCCAAAAG GTGTCCGCCA CACTATGATG 
TTTAGTGCTA CTTTTCCTAA GGAAATACAG ATGCTGGCCC GTGATTTCTT AGATGAGTAC 
ATATTTCTGG CTGTAGGAAG AGTTGGGTCT ACTTCAGAGA ACATCACACA AAAAGTGGTT 
TGGGTGGAGG AGATAGACAA AAGATCATTT CTGCTTGACC TCCTAAATGC AACAGGCAAG 
GATTCCCTGA CTCTAGTGTT TGTGGAGACC AAAAAGGGTG CAGATTCGCT GGAGGATTTC 
TTATACCATG AAGGATATGC CTGTACCAGT ATCCATGGAG ACCGTTCTCA GAGAGATAGG 
GAAGAGGCCC TTCACCAGTT CCGCTCAGGA AAAAGCCCAA TTCTAGTAGC TACAGCAGTA 
GCAGCAAGAG GACTGGATAT TTCAAATGTG AAGCATGTTA TTAATTTTGA CCTGCCTAGT 
GATATCGAAG AATATGTGCA TCGCATAGGC CGTACAGGGC GTGTAGGAAA CCTTGGTCTT 
GCCACCTCAT TCTTTAATGA AAGAAATATA AATATTACTA AGGATTTACT GGATCTTCTT 
GTTGAAGCAA AACAAGAAGT GCCATCTTGG TTGGAAAACA TGGCTTTTGA ACACCACTAT 
AAGGGTAGCA GCCGTGGACG TTCTAAGAGC AGTCGATTTA GTGGAGGGTT TGGTGCCAGA 
GACTATCGAC AGAGTAGCGG CGCCAGCAGT TCCAGCTTCA GCAGCAGCCG CGCAAGCAGC 
AGCCGGAGTG GTGGAGGTGG CCATGGCAGC AGCAGAGGAT TTGGTGGAGG TGGCTATGGA 
GGCTTTTACA ACAGTGATGG ATACGGAGGA AATTACAACT CCCAGGGGGT TGACTGGTGG 
GGAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256714.1
CDS	103..2091
Translation	MSHVAVENALGLDQQFAGLDLNSSDNQTGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRGDSRGKSSFFGDRGSGSRGRFDDRGRGDYDGIGGRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGAEIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEEIDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAFEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN

Target ORF information:

RefSeq Version	XM_006256652.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 3, X-linked (Ddx3x), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256652.3

ORF Insert Sequence:

ATGAGTCATG TGGCAGTGGA AAATGCGCTC GGGCTGGACC AGCAGTTTGC TGGCCTAGAC 
CTGAACTCTT CAGATAATCA GACTGGAGGA AGTACAGCAA GCAAAGGGCG CTATATCCCT 
CCTCATTTAA GGAACAGAGA AGCTACTAAA GGATTCTATG ACAAAGACAG TTCAGGGTGG 
AGTTCTAGTA AAGATAAAGA TGCATACAGC AGCTTTGGAT CCCGGGGTGA CTCACGAGGG 
AAGTCTAGCT TCTTTGGTGA TCGTGGAAGT GGATCAAGGG GAAGGTTTGA TGATCGTGGA 
CGGGGAGACT ATGATGGCAT TGGTGGCCGT GGAGATAGGA GTGGCTTTGG CAAATTTGAA 
AGAGGTGGAA ATAGTCGCTG GTGTGATAAA TCAGATGAAG ATGACTGGTC AAAGCCACTC 
CCACCAAGTG AACGCTTGGA ACAGGAACTC TTTTCTGGAG GCAATACTGG GATTAACTTT 
GAGAAATATG ATGACATTCC AGTTGAAGCA ACAGGCAACA ACTGTCCTCC ACATATTGAA 
AGTTTCAGTG ATGTTGAGAT GGGAGAAATT ATTATGGGAA ACATTGAGCT TACTCGTTAT 
ACTCGTCCAA CTCCAGTGCA GAAGCATGCT ATTCCTATTA TCAAAGAGAA AAGAGACTTG 
ATGGCTTGTG CTCAAACAGG CTCTGGAAAA ACTGCAGCAT TTCTCTTGCC CATCTTGAGT 
CAGATTTATG CTGATGGTCC CGGAGAGGCT CTGAGGGCCA TGAAGGAAAA TGGAAGATAT 
GGGCGTCGTA AACAGTATCC AATCTCCTTG GTACTGGCAC CAACGAGAGA ATTGGCAGTG 
CAGATCTATG AGGAAGCCAG AAAATTCTCA TACCGGTCTA GAGTCCGTCC TTGCGTGGTT 
TATGGTGGTG CTGAAATTGG TCAGCAGATT CGAGACTTAG AACGTGGATG CCACTTGTTA 
GTAGCCACTC CAGGACGTCT AGTGGACATG ATGGAGAGAG GGAAGATCGG GCTAGACTTC 
TGCAAATACC TGGTGTTAGA TGAAGCTGAC CGGATGTTAG ATATGGGGTT TGAACCTCAG 
ATACGTAGAA TAGTTGAACA AGACACTATG CCTCCAAAAG GTGTCCGCCA CACTATGATG 
TTTAGTGCTA CTTTTCCTAA GGAAATACAG ATGCTGGCCC GTGATTTCTT AGATGAGTAC 
ATATTTCTGG CTGTAGGAAG AGTTGGGTCT ACTTCAGAGA ACATCACACA AAAAGTGGTT 
TGGGTGGAGG AGATAGACAA AAGATCATTT CTGCTTGACC TCCTAAATGC AACAGGCAAG 
GATTCCCTGA CTCTAGTGTT TGTGGAGACC AAAAAGGGTG CAGATTCGCT GGAGGATTTC 
TTATACCATG AAGGATATGC CTGTACCAGT ATCCATGGAG ACCGTTCTCA GAGAGATAGG 
GAAGAGGCCC TTCACCAGTT CCGCTCAGGA AAAAGCCCAA TTCTAGTAGC TACAGCAGTA 
GCAGCAAGAG GACTGGATAT TTCAAATGTG AAGCATGTTA TTAATTTTGA CCTGCCTAGT 
GATATCGAAG AATATGTGCA TCGCATAGGC CGTACAGGGC GTGTAGGAAA CCTTGGTCTT 
GCCACCTCAT TCTTTAATGA AAGAAATATA AATATTACTA AGGATTTACT GGATCTTCTT 
GTTGAAGCAA AACAAGAAGT GCCATCTTGG TTGGAAAACA TGGCTTTTGA ACACCACTAT 
AAGGGTAGCA GCCGTGGACG TTCTAAGAGC AGTCGATTTA GTGGAGGGTT TGGTGCCAGA 
GACTATCGAC AGAGTAGCGG CGCCAGCAGT TCCAGCTTCA GCAGCAGCCG CGCAAGCAGC 
AGCCGGAGTG GTGGAGGTGG CCATGGCAGC AGCAGAGGAT TTGGTGGAGG TGGCTATGGA 
GGCTTTTACA ACAGTGATGG ATACGGAGGA AATTACAACT CCCAGGGGGT TGACTGGTGG 
GGAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx3x ( XM_006256653.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256715.1 Rattus norvegicus DEAD-box helicase 3, X-linked (Ddx3x), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39572
Clone ID Related Accession (Same CDS sequence)	XM_006256653.3
Accession Version	XM_006256653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent RNA helicase DDX3X isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCATG TGGCAGTGGA AAATGCGCTC GGGCTGGACC AGCAGTTTGC TGGCCTAGAC 
CTGAACTCTT CAGATAATCA GACTGGAGGA AGTACAGCAA GCAGGCGCTA TATCCCTCCT 
CATTTAAGGA ACAGAGAAGC TACTAAAGGA TTCTATGACA AAGACAGTTC AGGGTGGAGT 
TCTAGTAAAG ATAAAGATGC ATACAGCAGC TTTGGATCCC GGGGTGACTC ACGAGGGAAG 
TCTAGCTTCT TTGGTGATCG TGGAAGTGGA TCAAGGGGAA GGTTTGATGA TCGTGGACGG 
GGAGACTATG ATGGCATTGG TGGCCGTGGA GATAGGAGTG GCTTTGGCAA ATTTGAAAGA 
GGTGGAAATA GTCGCTGGTG TGATAAATCA GATGAAGATG ACTGGTCAAA GCCACTCCCA 
CCAAGTGAAC GCTTGGAACA GGAACTCTTT TCTGGAGGCA ATACTGGGAT TAACTTTGAG 
AAATATGATG ACATTCCAGT TGAAGCAACA GGCAACAACT GTCCTCCACA TATTGAAAGT 
TTCAGTGATG TTGAGATGGG AGAAATTATT ATGGGAAACA TTGAGCTTAC TCGTTATACT 
CGTCCAACTC CAGTGCAGAA GCATGCTATT CCTATTATCA AAGAGAAAAG AGACTTGATG 
GCTTGTGCTC AAACAGGCTC TGGAAAAACT GCAGCATTTC TCTTGCCCAT CTTGAGTCAG 
ATTTATGCTG ATGGTCCCGG AGAGGCTCTG AGGGCCATGA AGGAAAATGG AAGATATGGG 
CGTCGTAAAC AGTATCCAAT CTCCTTGGTA CTGGCACCAA CGAGAGAATT GGCAGTGCAG 
ATCTATGAGG AAGCCAGAAA ATTCTCATAC CGGTCTAGAG TCCGTCCTTG CGTGGTTTAT 
GGTGGTGCTG AAATTGGTCA GCAGATTCGA GACTTAGAAC GTGGATGCCA CTTGTTAGTA 
GCCACTCCAG GACGTCTAGT GGACATGATG GAGAGAGGGA AGATCGGGCT AGACTTCTGC 
AAATACCTGG TGTTAGATGA AGCTGACCGG ATGTTAGATA TGGGGTTTGA ACCTCAGATA 
CGTAGAATAG TTGAACAAGA CACTATGCCT CCAAAAGGTG TCCGCCACAC TATGATGTTT 
AGTGCTACTT TTCCTAAGGA AATACAGATG CTGGCCCGTG ATTTCTTAGA TGAGTACATA 
TTTCTGGCTG TAGGAAGAGT TGGGTCTACT TCAGAGAACA TCACACAAAA AGTGGTTTGG 
GTGGAGGAGA TAGACAAAAG ATCATTTCTG CTTGACCTCC TAAATGCAAC AGGCAAGGAT 
TCCCTGACTC TAGTGTTTGT GGAGACCAAA AAGGGTGCAG ATTCGCTGGA GGATTTCTTA 
TACCATGAAG GATATGCCTG TACCAGTATC CATGGAGACC GTTCTCAGAG AGATAGGGAA 
GAGGCCCTTC ACCAGTTCCG CTCAGGAAAA AGCCCAATTC TAGTAGCTAC AGCAGTAGCA 
GCAAGAGGAC TGGATATTTC AAATGTGAAG CATGTTATTA ATTTTGACCT GCCTAGTGAT 
ATCGAAGAAT ATGTGCATCG CATAGGCCGT ACAGGGCGTG TAGGAAACCT TGGTCTTGCC 
ACCTCATTCT TTAATGAAAG AAATATAAAT ATTACTAAGG ATTTACTGGA TCTTCTTGTT 
GAAGCAAAAC AAGAAGTGCC ATCTTGGTTG GAAAACATGG CTTTTGAACA CCACTATAAG 
GGTAGCAGCC GTGGACGTTC TAAGAGCAGT CGATTTAGTG GAGGGTTTGG TGCCAGAGAC 
TATCGACAGA GTAGCGGCGC CAGCAGTTCC AGCTTCAGCA GCAGCCGCGC AAGCAGCAGC 
CGGAGTGGTG GAGGTGGCCA TGGCAGCAGC AGAGGATTTG GTGGAGGTGG CTATGGAGGC 
TTTTACAACA GTGATGGATA CGGAGGAAAT TACAACTCCC AGGGGGTTGA CTGGTGGGGA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256715.1
CDS	92..2077
Translation	MSHVAVENALGLDQQFAGLDLNSSDNQTGGSTASRRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRGDSRGKSSFFGDRGSGSRGRFDDRGRGDYDGIGGRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGAEIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEEIDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAFEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN

Target ORF information:

RefSeq Version	XM_006256653.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 3, X-linked (Ddx3x), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256653.3

ORF Insert Sequence:

ATGAGTCATG TGGCAGTGGA AAATGCGCTC GGGCTGGACC AGCAGTTTGC TGGCCTAGAC 
CTGAACTCTT CAGATAATCA GACTGGAGGA AGTACAGCAA GCAGGCGCTA TATCCCTCCT 
CATTTAAGGA ACAGAGAAGC TACTAAAGGA TTCTATGACA AAGACAGTTC AGGGTGGAGT 
TCTAGTAAAG ATAAAGATGC ATACAGCAGC TTTGGATCCC GGGGTGACTC ACGAGGGAAG 
TCTAGCTTCT TTGGTGATCG TGGAAGTGGA TCAAGGGGAA GGTTTGATGA TCGTGGACGG 
GGAGACTATG ATGGCATTGG TGGCCGTGGA GATAGGAGTG GCTTTGGCAA ATTTGAAAGA 
GGTGGAAATA GTCGCTGGTG TGATAAATCA GATGAAGATG ACTGGTCAAA GCCACTCCCA 
CCAAGTGAAC GCTTGGAACA GGAACTCTTT TCTGGAGGCA ATACTGGGAT TAACTTTGAG 
AAATATGATG ACATTCCAGT TGAAGCAACA GGCAACAACT GTCCTCCACA TATTGAAAGT 
TTCAGTGATG TTGAGATGGG AGAAATTATT ATGGGAAACA TTGAGCTTAC TCGTTATACT 
CGTCCAACTC CAGTGCAGAA GCATGCTATT CCTATTATCA AAGAGAAAAG AGACTTGATG 
GCTTGTGCTC AAACAGGCTC TGGAAAAACT GCAGCATTTC TCTTGCCCAT CTTGAGTCAG 
ATTTATGCTG ATGGTCCCGG AGAGGCTCTG AGGGCCATGA AGGAAAATGG AAGATATGGG 
CGTCGTAAAC AGTATCCAAT CTCCTTGGTA CTGGCACCAA CGAGAGAATT GGCAGTGCAG 
ATCTATGAGG AAGCCAGAAA ATTCTCATAC CGGTCTAGAG TCCGTCCTTG CGTGGTTTAT 
GGTGGTGCTG AAATTGGTCA GCAGATTCGA GACTTAGAAC GTGGATGCCA CTTGTTAGTA 
GCCACTCCAG GACGTCTAGT GGACATGATG GAGAGAGGGA AGATCGGGCT AGACTTCTGC 
AAATACCTGG TGTTAGATGA AGCTGACCGG ATGTTAGATA TGGGGTTTGA ACCTCAGATA 
CGTAGAATAG TTGAACAAGA CACTATGCCT CCAAAAGGTG TCCGCCACAC TATGATGTTT 
AGTGCTACTT TTCCTAAGGA AATACAGATG CTGGCCCGTG ATTTCTTAGA TGAGTACATA 
TTTCTGGCTG TAGGAAGAGT TGGGTCTACT TCAGAGAACA TCACACAAAA AGTGGTTTGG 
GTGGAGGAGA TAGACAAAAG ATCATTTCTG CTTGACCTCC TAAATGCAAC AGGCAAGGAT 
TCCCTGACTC TAGTGTTTGT GGAGACCAAA AAGGGTGCAG ATTCGCTGGA GGATTTCTTA 
TACCATGAAG GATATGCCTG TACCAGTATC CATGGAGACC GTTCTCAGAG AGATAGGGAA 
GAGGCCCTTC ACCAGTTCCG CTCAGGAAAA AGCCCAATTC TAGTAGCTAC AGCAGTAGCA 
GCAAGAGGAC TGGATATTTC AAATGTGAAG CATGTTATTA ATTTTGACCT GCCTAGTGAT 
ATCGAAGAAT ATGTGCATCG CATAGGCCGT ACAGGGCGTG TAGGAAACCT TGGTCTTGCC 
ACCTCATTCT TTAATGAAAG AAATATAAAT ATTACTAAGG ATTTACTGGA TCTTCTTGTT 
GAAGCAAAAC AAGAAGTGCC ATCTTGGTTG GAAAACATGG CTTTTGAACA CCACTATAAG 
GGTAGCAGCC GTGGACGTTC TAAGAGCAGT CGATTTAGTG GAGGGTTTGG TGCCAGAGAC 
TATCGACAGA GTAGCGGCGC CAGCAGTTCC AGCTTCAGCA GCAGCCGCGC AAGCAGCAGC 
CGGAGTGGTG GAGGTGGCCA TGGCAGCAGC AGAGGATTTG GTGGAGGTGG CTATGGAGGC 
TTTTACAACA GTGATGGATA CGGAGGAAAT TACAACTCCC AGGGGGTTGA CTGGTGGGGA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA Remodeling Activity of DEAD Box Proteins Tuned by Protein Concentration, RNA Length, and ATP.
Molecular cell63(5)865-76(2016 Sep)
Kim Y,Myong S

The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
Molecular and cellular biology35(17)2918-31(2015 Sep)
Zhang Y,You J,Wang X,Weber J

Mutations in DDX3X Are a Common Cause of Unexplained Intellectual Disability with Gender-Specific Effects on Wnt Signaling.
American journal of human genetics97(2)343-52(2015 Aug)
Snijders Blok L,Madsen E,Juusola J,Gilissen C,Baralle D,Reijnders MR,Venselaar H,Helsmoortel C,Cho MT,Hoischen A,Vissers LE,Koemans TS,Wissink-Lindhout W,Eichler EE,Romano C,Van Esch H,Stumpel C,Vreeburg M,Smeets E,Oberndorff K,van Bon BW,Shaw M,Gecz J,Haan E,Bienek M,Jensen C,Loeys BL,Van Dijck A,Innes AM,Racher H,Vermeer S,Di Donato N,Rump A,Tatton-Brown K,Parker MJ,Henderson A,Lynch SA,Fryer A,Ross A,Vasudevan P,Kini U,Newbury-Ecob R,Chandler K,Male A,,Dijkstra S,Schieving J,Giltay J,van Gassen KL,Schuurs-Hoeijmakers J,Tan PL,Pediaditakis I,Haas SA,Retterer K,Reed P,Monaghan KG,Haverfield E,Natowicz M,Myers A,Kruer MC,Stein Q,Strauss KA,Brigatti KW,Keating K,Burton BK,Kim KH,Charrow J,Norman J,Foster-Barber A,Kline AD,Kimball A,Zackai E,Harr M,Fox J,McLaughlin J,Lindstrom K,Haude KM,van Roozendaal K,Brunner H,Chung WK,Kooy RF,Pfundt R,Kalscheuer V,Mehta SG,Katsanis N,Kleefstra T

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology88(18)10340-53(2014 Sep)
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP

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Ddx3x cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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