Chpf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chpf
Entrez Gene ID	316533
Full Name	chondroitin polymerizing factor
Synonyms	D1bwg1363e
General protein information	Preferred Names chondroitin polymerizing factor Names chondroitin sulfate synthase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q33

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005906.1	NP_001005906.1	chondroitin sulfate synthase 2
XM_006245228.2	XP_006245290.1	chondroitin sulfate synthase 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chpf	NP_001005906.1
Danio rerio (zebrafish)	chpfa	XP_002663375.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001050	XP_318249.4
Canis lupus familiaris (dog)	CHPF	XP_003640243.1
Drosophila melanogaster (fruit fly)	CG43313	NP_572853.2
Bos taurus (cattle)	CHPF	NP_001192680.1
Mus musculus (house mouse)	Chpf	NP_001001566.1
Danio rerio (zebrafish)	LOC794660	XP_001923418.1
Caenorhabditis elegans (roundworm)	mig-22	NP_498934.1
Homo sapiens (human)	CHPF	NP_078812.2
Pan troglodytes (chimpanzee)	CHPF	XP_003309517.1
	CHPF	XP_001104808.1
Gallus gallus (chicken)	CHPF	XP_001232317.2
Xenopus tropicalis (tropical clawed frog)	chpf	XP_002942593.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene network and pathway analysis of mice with conditional ablation of Dicer in post-mitotic neurons.
Dorval V, Smith PY, Delay C, Calvo E, Planel E, Zommer N, Bu?e L, H?bert SS
PloS one7(8)e44060(2012)

Chondroitin sulfate synthase-2 is necessary for chain extension of chondroitin sulfate but not critical for skeletal development.
Ogawa H, Hatano S, Sugiura N, Nagai N, Sato T, Shimizu K, Kimata K, Narimatsu H, Watanabe H
PloS one7(8)e43806(2012)

Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization.
Kitagawa H, Izumikawa T, Uyama T, Sugahara K
The Journal of biological chemistry278(26)23666-71(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chpf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chpf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10809	NM_001005906.1 Latest version!	Rattus norvegicus chondroitin polymerizing factor (Chpf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa32394	XM_006245228.2 Latest version!	Rattus norvegicus chondroitin polymerizing factor (Chpf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chpf ( NM_001005906.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001005906.1 Rattus norvegicus chondroitin polymerizing factor (Chpf), mRNA.

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CloneID	ORa10809
Clone ID Related Accession (Same CDS sequence)	NM_001005906.1
Accession Version	NM_001005906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	chondroitin sulfate synthase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083617.1. On Oct 23, 2004 this sequence version replaced XM_237306.2. ##Evidence-Data-START## Transcript exon combination :: BC083617.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCGGGCGT CGCTGCTGCT GTCCGTGCTG CGGCCTGCGG GGCCGGTGGC CGTGGGCATC 
TCTCTGGGCT TCACCCTGAG CTTACTCAGC GTCACCTGGG TGGAGGAGCC TTGCGGACCC 
GGACCACCCC AACCCGGCGA CTCTGAACTA CCGCCGCGCG GCAACACCAA CGCGGCGCGC 
CGGCCCAACT CGGTGCAGCC CGGAGCGGAG CGCGAGAGGC CCGGGGCCGA CGAAAGCTGG 
GAGCCTCGTG TCTTGCCCTA CCATCCCGCC CAGCCAGGCC AGGCTACCAA GAAGGCCGTC 
AGAACTCGGT ATATCAGCAC GGAACTGGGC ATCAGGCAGA AGCTGCTAGT GGCAGTGCTG 
ACCTCACAGG CCACGTTGCC TACACTGGGT GTGGCTGTAA ACCGAACTCT GGGACACCGA 
TTGGAGCATG TAGTGTTCCT GACCGGTGCG AGGGGCCGCC GGACACCATC AGGCATGGCG 
GTGGTGGCAC TGGGCGAAGA GCGGCCCATC GGACACCTGC ACCTGGCGCT GCGCCACCTG 
CTGGAGCAAC ACGGCGATGA CTTTGACTGG TTTTTCCTAG TGCCTGATGC CACCTACACT 
GAGGCGCATG GACTGGACCG CCTAACTGGC CACCTCAGCC TTGCTTCAGC AACCCACCTC 
TATCTTGGCC GACCCCAGGA CTTCATCGGT GGAGAGACTA CCCCAGGCCG TTACTGCCAC 
GGGGGCTTTG GAGTGTTGCT GTCTCGCACA CTACTACAGC AACTGCGCCC CCACCTGGAA 
AGCTGCCGTA ATGACATCGT CAGTGCTCGC CCAGATGAGT GGTTGGGCCG GTGCATCCTT 
GATGCCACAG GCATTGGCTG TACTGGTCAC CATGAGGGAA TGCACTACAA CTATCTGGAA 
CTGAGCCCTG GGGAACCTGT GCAGGAGGGG GACCCTCGTT TCCGCAGCGC CTTGACAGCC 
CACCCTGTGC ATGACCCTGT GCACATGTAC CAGCTGCACA AAGCTTTTGC CCGGGCTGAG 
CTGGACCGCA CATACCAGGA GATTCAAGAA CTGCAGTGGG AGATCCAGAA TACCAGCAGA 
CTGGCTGCTG ATGGGGAGAG GGCTTCTACC TGGCCAGTGG GCATTCCAGC GCCGTCTCGC 
CCTGCCTCGC GCTTTGAGGT TCTGCGCTGG GACTACTTCA CGGAACAGCA CACTTTCTCC 
TGTGCTGACG GCTCACCCCG CTGCCTGCTG CGTGGGGCCG ACCAGGCCGA TGTGGCTGAT 
GTCCTAGGAA CAGCCTTAGA GGAGCTCAAC CGCCGTTACC AGCCAGCACT GCGGCTCCAG 
AAGCAACAGC TGGTGAATGG CTACCGGCGT TTTGATCCAG CCCGAGGTAT GGAGTACACA 
CTAGACTTGC AGCTGGAAGC GCTGACGCCC CAGGGTGGCC GCTGGCCCCT CACCCACAGG 
GTACAGCTCC TTCGGCCCTT GAGCCGAGTG GAGATCTTGC CTGTGCCCTA TGTCACCGAG 
GCTTCTCGGG TCACTGTGCT GCTGCCTCTG GCTGCAGCAG AGTGTGACCT GGCATCTGGC 
TTCCTAGAAG CCTTTGCCAC TGCAGCACTG GAACCTGGCG ATGCCGCAGC CTTGACCCTG 
CTGCTGCTGT ATGAGCCACG CCAGGCCCAG CGGGCAGCCC ACGCAGATGT CTTCGCACCC 
GTCAAAGCCC ACGTGGCCGA GCTAGAGCGG CGTTTCCCTG GTGCCCGAGT GCCCTGGCTC 
AGTGTACAGA CATCCGCGCC CTCTCCGCTG CGCCTCATGG ATCTACTCTC CAAGAAGCAC 
CCGCTGGACA CTCTGTTCCT GCTGGCCGGG CCAGACACAG TGCTCACACC CGACTTCCTG 
AACCGCTGCC GCATGCACGC CATCTCGGGC TGGCAAGCCT TCTTTCCCAT GCACTTCCAG 
GCCTTCCACC CTGCTGTGGC TCCACCTCAG GGCCCTGGGC CACCAGAACT GGGCCGTGAC 
ACCGGCCACT TTGATCGTCA GGCTGCCAGC GAGGCATGCT TCTACAACTC CGACTATGTG 
GCGGCCCGTG GACGGCTGGT GGCAGTCTCC GAGCAGGAGG AGGAGCTGCT GGAGAGCCTG 
GATGTGTATG AGTTGTTTCT ACGCTTCTCT AGCTTGCATG TGCTGAGGGC AGTGGAACCA 
GCCTTGCTGC AGCGCTACCG GGCCCAGCCA TGCAGCGCTC GGCTCAGCGA GGACCTTTAT 
CACCGCTGTC GCCAGAGCGT GCTTGAGGGC CTTGGCTCCC GCACCCAGCT TGCCATGCTG 
CTCTTTGAAC AGGAACAGGG TAACAGCACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005906.1
CDS	207..2519
Translation	MRASLLLSVLRPAGPVAVGISLGFTLSLLSVTWVEEPCGPGPPQPGDSELPPRGNTNAARRPNSVQPGAERERPGADESWEPRVLPYHPAQPGQATKKAVRTRYISTELGIRQKLLVAVLTSQATLPTLGVAVNRTLGHRLEHVVFLTGARGRRTPSGMAVVALGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDATYTEAHGLDRLTGHLSLASATHLYLGRPQDFIGGETTPGRYCHGGFGVLLSRTLLQQLRPHLESCRNDIVSARPDEWLGRCILDATGIGCTGHHEGMHYNYLELSPGEPVQEGDPRFRSALTAHPVHDPVHMYQLHKAFARAELDRTYQEIQELQWEIQNTSRLAADGERASTWPVGIPAPSRPASRFEVLRWDYFTEQHTFSCADGSPRCLLRGADQADVADVLGTALEELNRRYQPALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRWPLTHRVQLLRPLSRVEILPVPYVTEASRVTVLLPLAAAECDLASGFLEAFATAALEPGDAAALTLLLLYEPRQAQRAAHADVFAPVKAHVAELERRFPGARVPWLSVQTSAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGHFDRQAASEACFYNSDYVAARGRLVAVSEQEEELLESLDVYELFLRFSSLHVLRAVEPALLQRYRAQPCSARLSEDLYHRCRQSVLEGLGSRTQLAMLLFEQEQGNST

Target ORF information:

RefSeq Version	NM_001005906.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chondroitin polymerizing factor (Chpf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005906.1

ORF Insert Sequence:

ATGCGGGCGT CGCTGCTGCT GTCCGTGCTG CGGCCTGCGG GGCCGGTGGC CGTGGGCATC 
TCTCTGGGCT TCACCCTGAG CTTACTCAGC GTCACCTGGG TGGAGGAGCC TTGCGGACCC 
GGACCACCCC AACCCGGCGA CTCTGAACTA CCGCCGCGCG GCAACACCAA CGCGGCGCGC 
CGGCCCAACT CGGTGCAGCC CGGAGCGGAG CGCGAGAGGC CCGGGGCCGA CGAAAGCTGG 
GAGCCTCGTG TCTTGCCCTA CCATCCCGCC CAGCCAGGCC AGGCTACCAA GAAGGCCGTC 
AGAACTCGGT ATATCAGCAC GGAACTGGGC ATCAGGCAGA AGCTGCTAGT GGCAGTGCTG 
ACCTCACAGG CCACGTTGCC TACACTGGGT GTGGCTGTAA ACCGAACTCT GGGACACCGA 
TTGGAGCATG TAGTGTTCCT GACCGGTGCG AGGGGCCGCC GGACACCATC AGGCATGGCG 
GTGGTGGCAC TGGGCGAAGA GCGGCCCATC GGACACCTGC ACCTGGCGCT GCGCCACCTG 
CTGGAGCAAC ACGGCGATGA CTTTGACTGG TTTTTCCTAG TGCCTGATGC CACCTACACT 
GAGGCGCATG GACTGGACCG CCTAACTGGC CACCTCAGCC TTGCTTCAGC AACCCACCTC 
TATCTTGGCC GACCCCAGGA CTTCATCGGT GGAGAGACTA CCCCAGGCCG TTACTGCCAC 
GGGGGCTTTG GAGTGTTGCT GTCTCGCACA CTACTACAGC AACTGCGCCC CCACCTGGAA 
AGCTGCCGTA ATGACATCGT CAGTGCTCGC CCAGATGAGT GGTTGGGCCG GTGCATCCTT 
GATGCCACAG GCATTGGCTG TACTGGTCAC CATGAGGGAA TGCACTACAA CTATCTGGAA 
CTGAGCCCTG GGGAACCTGT GCAGGAGGGG GACCCTCGTT TCCGCAGCGC CTTGACAGCC 
CACCCTGTGC ATGACCCTGT GCACATGTAC CAGCTGCACA AAGCTTTTGC CCGGGCTGAG 
CTGGACCGCA CATACCAGGA GATTCAAGAA CTGCAGTGGG AGATCCAGAA TACCAGCAGA 
CTGGCTGCTG ATGGGGAGAG GGCTTCTACC TGGCCAGTGG GCATTCCAGC GCCGTCTCGC 
CCTGCCTCGC GCTTTGAGGT TCTGCGCTGG GACTACTTCA CGGAACAGCA CACTTTCTCC 
TGTGCTGACG GCTCACCCCG CTGCCTGCTG CGTGGGGCCG ACCAGGCCGA TGTGGCTGAT 
GTCCTAGGAA CAGCCTTAGA GGAGCTCAAC CGCCGTTACC AGCCAGCACT GCGGCTCCAG 
AAGCAACAGC TGGTGAATGG CTACCGGCGT TTTGATCCAG CCCGAGGTAT GGAGTACACA 
CTAGACTTGC AGCTGGAAGC GCTGACGCCC CAGGGTGGCC GCTGGCCCCT CACCCACAGG 
GTACAGCTCC TTCGGCCCTT GAGCCGAGTG GAGATCTTGC CTGTGCCCTA TGTCACCGAG 
GCTTCTCGGG TCACTGTGCT GCTGCCTCTG GCTGCAGCAG AGTGTGACCT GGCATCTGGC 
TTCCTAGAAG CCTTTGCCAC TGCAGCACTG GAACCTGGCG ATGCCGCAGC CTTGACCCTG 
CTGCTGCTGT ATGAGCCACG CCAGGCCCAG CGGGCAGCCC ACGCAGATGT CTTCGCACCC 
GTCAAAGCCC ACGTGGCCGA GCTAGAGCGG CGTTTCCCTG GTGCCCGAGT GCCCTGGCTC 
AGTGTACAGA CATCCGCGCC CTCTCCGCTG CGCCTCATGG ATCTACTCTC CAAGAAGCAC 
CCGCTGGACA CTCTGTTCCT GCTGGCCGGG CCAGACACAG TGCTCACACC CGACTTCCTG 
AACCGCTGCC GCATGCACGC CATCTCGGGC TGGCAAGCCT TCTTTCCCAT GCACTTCCAG 
GCCTTCCACC CTGCTGTGGC TCCACCTCAG GGCCCTGGGC CACCAGAACT GGGCCGTGAC 
ACCGGCCACT TTGATCGTCA GGCTGCCAGC GAGGCATGCT TCTACAACTC CGACTATGTG 
GCGGCCCGTG GACGGCTGGT GGCAGTCTCC GAGCAGGAGG AGGAGCTGCT GGAGAGCCTG 
GATGTGTATG AGTTGTTTCT ACGCTTCTCT AGCTTGCATG TGCTGAGGGC AGTGGAACCA 
GCCTTGCTGC AGCGCTACCG GGCCCAGCCA TGCAGCGCTC GGCTCAGCGA GGACCTTTAT 
CACCGCTGTC GCCAGAGCGT GCTTGAGGGC CTTGGCTCCC GCACCCAGCT TGCCATGCTG 
CTCTTTGAAC AGGAACAGGG TAACAGCACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chpf ( XM_006245228.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245290.1 Rattus norvegicus chondroitin polymerizing factor (Chpf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32394
Clone ID Related Accession (Same CDS sequence)	XM_006245228.2
Accession Version	XM_006245228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	chondroitin sulfate synthase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245228.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG TGGCACTGGG CGAAGAGCGG CCCATCGGAC ACCTGCACCT GGCGCTGCGC 
CACCTGCTGG AGCAACACGG CGATGACTTT GACTGGTTTT TCCTAGTGCC TGATGCCACC 
TACACTGAGG CGCATGGACT GGACCGCCTA ACTGGCCACC TCAGCCTTGC TTCAGCAACC 
CACCTCTATC TTGGCCGACC CCAGGACTTC ATCGGTGGAG AGACTACCCC AGGCCGTTAC 
TGCCACGGGG GCTTTGGAGT GTTGCTGTCT CGCACACTAC TACAGCAACT GCGCCCCCAC 
CTGGAAAGCT GCCGTAATGA CATCGTCAGT GCTCGCCCAG ATGAGTGGTT GGGCCGGTGC 
ATCCTTGATG CCACAGGCAT TGGCTGTACT GGTCACCATG AGGGAATGCA CTACAACTAT 
CTGGAACTGA GCCCTGGGGA ACCTGTGCAG GAGGGGGACC CTCGTTTCCG CAGCGCCTTG 
ACAGCCCACC CTGTGCATGA CCCTGTGCAC ATGTACCAGC TGCACAAAGC TTTTGCCCGG 
GCTGAGCTGG ACCGCACATA CCAGGAGATT CAAGAACTGC AGTGGGAGAT CCAGAATACC 
AGCAGACTGG CTGCTGATGG GGAGAGGGCT TCTACCTGGC CAGTGGGCAT TCCAGCGCCG 
TCTCGCCCTG CCTCGCGCTT TGAGGTTCTG CGCTGGGACT ACTTCACGGA ACAGCACACT 
TTCTCCTGTG CTGACGGCTC ACCCCGCTGC CTGCTGCGTG GGGCCGACCA GGCCGATGTG 
GCTGATGTCC TAGGAACAGC CTTAGAGGAG CTCAACCGCC GTTACCAGCC AGCACTGCGG 
CTCCAGAAGC AACAGCTGGT GAATGGCTAC CGGCGTTTTG ATCCAGCCCG AGGTATGGAG 
TACACACTAG ACTTGCAGCT GGAAGCGCTG ACGCCCCAGG GTGGCCGCTG GCCCCTCACC 
CACAGGGTAC AGCTCCTTCG GCCCTTGAGC CGAGTGGAGA TCTTGCCTGT GCCCTATGTC 
ACCGAGGCTT CTCGGGTCAC TGTGCTGCTG CCTCTGGCTG CAGCAGAGTG TGACCTGGCA 
TCTGGCTTCC TAGAAGCCTT TGCCACTGCA GCACTGGAAC CTGGCGATGC CGCAGCCTTG 
ACCCTGCTGC TGCTGTATGA GCCACGCCAG GCCCAGCGGG CAGCCCACGC AGATGTCTTC 
GCACCCGTCA AAGCCCACGT GGCCGAGCTA GAGCGGCGTT TCCCTGGTGC CCGAGTGCCC 
TGGCTCAGTG TACAGACATC CGCGCCCTCT CCGCTGCGCC TCATGGATCT ACTCTCCAAG 
AAGCACCCGC TGGACACTCT GTTCCTGCTG GCCGGGCCAG ACACAGTGCT CACACCCGAC 
TTCCTGAACC GCTGCCGCAT GCACGCCATC TCGGGCTGGC AAGCCTTCTT TCCCATGCAC 
TTCCAGGCCT TCCACCCTGC TGTGGCTCCA CCTCAGGGCC CTGGGCCACC AGAACTGGGC 
CGTGACACCG GCCACTTTGA TCGTCAGGCT GCCAGCGAGG CATGCTTCTA CAACTCCGAC 
TATGTGGCGG CCCGTGGACG GCTGGTGGCA GTCTCCGAGC AGGAGGAGGA GCTGCTGGAG 
AGCCTGGATG TGTATGAGTT GTTTCTACGC TTCTCTAGCT TGCATGTGCT GAGGGCAGTG 
GAACCAGCCT TGCTGCAGCG CTACCGGGCC CAGCCATGCA GCGCTCGGCT CAGCGAGGAC 
CTTTATCACC GCTGTCGCCA GAGCGTGCTT GAGGGCCTTG GCTCCCGCAC CCAGCTTGCC 
ATGCTGCTCT TTGAACAGGA ACAGGGTAAC AGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245290.1
CDS	405..2243
Translation	MAVVALGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDATYTEAHGLDRLTGHLSLASATHLYLGRPQDFIGGETTPGRYCHGGFGVLLSRTLLQQLRPHLESCRNDIVSARPDEWLGRCILDATGIGCTGHHEGMHYNYLELSPGEPVQEGDPRFRSALTAHPVHDPVHMYQLHKAFARAELDRTYQEIQELQWEIQNTSRLAADGERASTWPVGIPAPSRPASRFEVLRWDYFTEQHTFSCADGSPRCLLRGADQADVADVLGTALEELNRRYQPALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRWPLTHRVQLLRPLSRVEILPVPYVTEASRVTVLLPLAAAECDLASGFLEAFATAALEPGDAAALTLLLLYEPRQAQRAAHADVFAPVKAHVAELERRFPGARVPWLSVQTSAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGHFDRQAASEACFYNSDYVAARGRLVAVSEQEEELLESLDVYELFLRFSSLHVLRAVEPALLQRYRAQPCSARLSEDLYHRCRQSVLEGLGSRTQLAMLLFEQEQGNST

Target ORF information:

RefSeq Version	XM_006245228.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chondroitin polymerizing factor (Chpf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245228.2

ORF Insert Sequence:

ATGGCGGTGG TGGCACTGGG CGAAGAGCGG CCCATCGGAC ACCTGCACCT GGCGCTGCGC 
CACCTGCTGG AGCAACACGG CGATGACTTT GACTGGTTTT TCCTAGTGCC TGATGCCACC 
TACACTGAGG CGCATGGACT GGACCGCCTA ACTGGCCACC TCAGCCTTGC TTCAGCAACC 
CACCTCTATC TTGGCCGACC CCAGGACTTC ATCGGTGGAG AGACTACCCC AGGCCGTTAC 
TGCCACGGGG GCTTTGGAGT GTTGCTGTCT CGCACACTAC TACAGCAACT GCGCCCCCAC 
CTGGAAAGCT GCCGTAATGA CATCGTCAGT GCTCGCCCAG ATGAGTGGTT GGGCCGGTGC 
ATCCTTGATG CCACAGGCAT TGGCTGTACT GGTCACCATG AGGGAATGCA CTACAACTAT 
CTGGAACTGA GCCCTGGGGA ACCTGTGCAG GAGGGGGACC CTCGTTTCCG CAGCGCCTTG 
ACAGCCCACC CTGTGCATGA CCCTGTGCAC ATGTACCAGC TGCACAAAGC TTTTGCCCGG 
GCTGAGCTGG ACCGCACATA CCAGGAGATT CAAGAACTGC AGTGGGAGAT CCAGAATACC 
AGCAGACTGG CTGCTGATGG GGAGAGGGCT TCTACCTGGC CAGTGGGCAT TCCAGCGCCG 
TCTCGCCCTG CCTCGCGCTT TGAGGTTCTG CGCTGGGACT ACTTCACGGA ACAGCACACT 
TTCTCCTGTG CTGACGGCTC ACCCCGCTGC CTGCTGCGTG GGGCCGACCA GGCCGATGTG 
GCTGATGTCC TAGGAACAGC CTTAGAGGAG CTCAACCGCC GTTACCAGCC AGCACTGCGG 
CTCCAGAAGC AACAGCTGGT GAATGGCTAC CGGCGTTTTG ATCCAGCCCG AGGTATGGAG 
TACACACTAG ACTTGCAGCT GGAAGCGCTG ACGCCCCAGG GTGGCCGCTG GCCCCTCACC 
CACAGGGTAC AGCTCCTTCG GCCCTTGAGC CGAGTGGAGA TCTTGCCTGT GCCCTATGTC 
ACCGAGGCTT CTCGGGTCAC TGTGCTGCTG CCTCTGGCTG CAGCAGAGTG TGACCTGGCA 
TCTGGCTTCC TAGAAGCCTT TGCCACTGCA GCACTGGAAC CTGGCGATGC CGCAGCCTTG 
ACCCTGCTGC TGCTGTATGA GCCACGCCAG GCCCAGCGGG CAGCCCACGC AGATGTCTTC 
GCACCCGTCA AAGCCCACGT GGCCGAGCTA GAGCGGCGTT TCCCTGGTGC CCGAGTGCCC 
TGGCTCAGTG TACAGACATC CGCGCCCTCT CCGCTGCGCC TCATGGATCT ACTCTCCAAG 
AAGCACCCGC TGGACACTCT GTTCCTGCTG GCCGGGCCAG ACACAGTGCT CACACCCGAC 
TTCCTGAACC GCTGCCGCAT GCACGCCATC TCGGGCTGGC AAGCCTTCTT TCCCATGCAC 
TTCCAGGCCT TCCACCCTGC TGTGGCTCCA CCTCAGGGCC CTGGGCCACC AGAACTGGGC 
CGTGACACCG GCCACTTTGA TCGTCAGGCT GCCAGCGAGG CATGCTTCTA CAACTCCGAC 
TATGTGGCGG CCCGTGGACG GCTGGTGGCA GTCTCCGAGC AGGAGGAGGA GCTGCTGGAG 
AGCCTGGATG TGTATGAGTT GTTTCTACGC TTCTCTAGCT TGCATGTGCT GAGGGCAGTG 
GAACCAGCCT TGCTGCAGCG CTACCGGGCC CAGCCATGCA GCGCTCGGCT CAGCGAGGAC 
CTTTATCACC GCTGTCGCCA GAGCGTGCTT GAGGGCCTTG GCTCCCGCAC CCAGCTTGCC 
ATGCTGCTCT TTGAACAGGA ACAGGGTAAC AGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene network and pathway analysis of mice with conditional ablation of Dicer in post-mitotic neurons.
PloS one7(8)e44060(2012)
Dorval V,Smith PY,Delay C,Calvo E,Planel E,Zommer N,Bu?e L,H?bert SS

Chondroitin sulfate synthase-2 is necessary for chain extension of chondroitin sulfate but not critical for skeletal development.
PloS one7(8)e43806(2012)
Ogawa H,Hatano S,Sugiura N,Nagai N,Sato T,Shimizu K,Kimata K,Narimatsu H,Watanabe H

Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization.
The Journal of biological chemistry278(26)23666-71(2003 Jun)
Kitagawa H,Izumikawa T,Uyama T,Sugahara K

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Chpf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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