gRNAs
The following Sgo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sgo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa10493 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006244988.3 , NM_001271191.1 | |
| Accession Version | NM_001271191.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-01 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | shugoshin 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07067807.1. On or before Sep 7, 2012 this sequence version replaced XM_237176.4, XM_001068385.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001258120.1 |
| CDS | 1..3489 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001271191.1 |
1 | ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa10493 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006244988.3 , NM_001271191.1 | |
| Accession Version | XM_006244988.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | shugoshin 2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006245050.1 |
| CDS | 319..3807 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006244988.3 |
1 | ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa32235 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006244989.3 | |
| Accession Version | XM_006244989.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | shugoshin 2 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006245051.1 |
| CDS | 319..3648 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006244989.3 |
1 | ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Meikin is a conserved regulator of meiosis-I-specific kinetochore function. |
Sequential assembly of centromeric proteins in male mouse meiosis. |
Shugoshin-2 is essential for the completion of meiosis but not for mitotic cell division in mice. |
Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells. |
Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments. |
