Mmel1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mmel1
Entrez Gene ID	313755
Full Name	membrane metallo-endopeptidase-like 1
Synonyms	Mell1,NEP2,NEPII
General protein information	Preferred Names membrane metallo-endopeptidase-like 1 Names membrane metallo-endopeptidase-like 1 NEP2(m) NL2 mel transforming oncogene-like 1 neprilysin 2 neprilysin II
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_006239567.3	XP_006239629.1	membrane metallo-endopeptidase-like 1 isoform X1
XM_006239570.3	XP_006239632.1	membrane metallo-endopeptidase-like 1 isoform X3
NM_001107997.1	NP_001101467.1	membrane metallo-endopeptidase-like 1
XM_006239569.3	XP_006239631.1	membrane metallo-endopeptidase-like 1 isoform X2
XM_008764360.2	XP_008762582.1	membrane metallo-endopeptidase-like 1 isoform X4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MMEL1	XP_001233077.3
Danio rerio (zebrafish)	mmel1	XP_689191.3
Canis lupus familiaris (dog)	MMEL1	XP_005620485.1
Mus musculus (house mouse)	Mmel1	NP_038811.2
Xenopus tropicalis (tropical clawed frog)	mmel1	NP_001120567.1
Homo sapiens (human)	MMEL1	NP_258428.2
Drosophila melanogaster (fruit fly)	Nep4	NP_650904.3
Pan troglodytes (chimpanzee)	MMEL1	XP_001150860.1
Bos taurus (cattle)	MMEL1	NP_001180065.1
Rattus norvegicus (Norway rat)	Mmel1	NP_001101467.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003106	XP_312790.4
Caenorhabditis elegans (roundworm)	nep-2	NP_494343.2

Gene Ontology
Bibliography

Related articles in PubMed

Human neprilysin-2 (NEP2) and NEP display distinct subcellular localisations and substrate preferences.
Whyteside AR, Turner AJ
FEBS letters582(16)2382-6(2008 Jul)

The ultimate tryptophan residue of neprilysin 2 is not involved in protein maturation and enzymatic activity.
Voisin S, Ouimet T
Biochemical and biophysical research communications335(2)356-60(2005 Sep)

A three-dimensional model of the neprilysin 2 active site based on the X-ray structure of neprilysin. Identification of residues involved in substrate hydrolysis and inhibitor binding of neprilysin 2.
Voisin S, Rognan D, Gros C, Ouimet T
The Journal of biological chemistry279(44)46172-81(2004 Oct)

Ontogeny, regional and cellular distribution of the novel metalloprotease neprilysin 2 in the rat: a comparison with neprilysin and endothelin-converting enzyme-1.
Facchinetti P, Rose C, Schwartz JC, Ouimet T
Neuroscience118(3)627-39(2003)

Cell-specific activity of neprilysin 2 isoforms and enzymic specificity compared with neprilysin.
Rose C, Voisin S, Gros C, Schwartz JC, Ouimet T
The Biochemical journal363(Pt 3)697-705(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Modification of the structure of the C-terminal region will either impair substrate hydrolysis or completely abolish the secretion and enzymatic activity of the secreted isoform of rat NEP2.
Title: The ultimate tryptophan residue of neprilysin 2 is not involved in protein maturation and enzymatic activity.

A three-dimensional model of the neprilysin 2 active site based on the X-ray structure of neprilysin.
Title: A three-dimensional model of the neprilysin 2 active site based on the X-ray structure of neprilysin. Identification of residues involved in substrate hydrolysis and inhibitor binding of neprilysin 2.

The following Mmel1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mmel1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06458	NM_001107997.1 Latest version!	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29004	XM_006239567.3 Latest version!	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29006	XM_006239569.3 Latest version!	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29007	XM_006239570.3 Latest version!	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa47264	XM_008764360.2 Latest version!	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mmel1 ( NM_001107997.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101467.1 Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), mRNA.

Price & Availability↑

CloneID	ORa06458
Clone ID Related Accession (Same CDS sequence)	NM_001107997.1
Accession Version	NM_001107997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	membrane metallo-endopeptidase-like 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473968.1. On or before Oct 4, 2007 this sequence version replaced XM_233712.4, XM_001077229.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2689604, SAMN02642603 [ECO:0000350] ##Evidence-Data-END##

ATGGGGAAGT CGGAGAGCTC AGTGGGGATG ATGGAGAGAG CGGACAACTG TGGGAGGAGG 
CGCCTAGGCT TCGTGGAGTG TGGGCTGCTG GTACTGCTGA CACTGCTGTT GATGGGAGCC 
ATAGTGACTC TGGGTGTCTT CTACAGCATA GGGAAGCAGC TGCCCCTCTT AAATAGCCTG 
CTGCACGTCT CCCGGCATGA GAGGACGGTT GTAAAACGAG CCGCCCTCAG AGATTCATCG 
CAGAAGAGTG ACATCTGTAC TACCCCAAGC TGCGTGATAG CAGCTGCCAG AATCCTCCAG 
AACATGGACC AGTCAAAGAA ACCCTGCGAC AACTTCTATC AGTATGCTTG CGGAGGCTGG 
CTACGGCACC ATGTGATCCC CGAGACCAAC TCCAGATACA GCGTCTTTGA CATCCTTCGG 
GATGAGCTGG AGGTCATCCT CAAAGGGGTG CTGGAGGATT CCTCTGTCCA GCACCGCCCA 
GCTGTGGAGA AGGCCAAGAC ACTGTACCGC TCCTGCATGA ACCAGAGTGT GATAGAGAAG 
AGAGACTCTG AGCCCCTGCT GAACGTCTTA GATATGATAG GAGGTTGGCC TGTAGCCATG 
GACAAGTGGA ATGAGACCAT GGGCCCCAAG TGGGAACTGG AGCGGCAGTT GGCTGTGTTG 
AACTCGCAGT TCAACAGGCG CGTCCTCATC GACCTCTTCA TCTGGAATGA TGACCAGAAC 
TCCAGCCGGC ACGTCATCTA CATAGACCAG CCCACCTTGG GCATGCCCTC CCGGGAGTAC 
TATTTCAAGG AAGACAGCCA CCGGGTACGG GAAGCCTACC TGCAGTTCAT GACATCAGTG 
GCCACTATGC TGAGGAGAGA CCTGAACCTG CCCGGGGAGA CCGATTTGGT GCAGGAGGAA 
ATGGCACAGG TGCTGCATCT GGAGACACAT CTGGCCAACG CCACGGTCCC CCAGGAGAAA 
AGGCATGATG TCACCGCCCT GTATCACCGA ATGGGCCTGG AGGAGCTGCA GGAAAGGTTT 
GGTCTGAAGG GGTTTAACTG GACTCTCTTC ATACAAAACG TGCTGTCTTC TGTGCAAGTT 
GAGCTGCTCC CGAATGAGGA GGTGGTGGTC TATGGCATCC CCTACCTGGA GAATCTTGAG 
GAGATCATTG ACGTCTTCCC AGCACAGACC TTGCAAAACT ACCTGGTGTG GCGCCTGGTG 
CTAGATCGCA TCGGCAGCCT GAGCCAGAGA TTCAAAGAAG CGCGTGTGGA CTACCGCAAG 
GCGCTGTACG GTACAACCAT GGAGGAAGTA CGCTGGCGGG AGTGTGTCAG CTATGTCAAC 
AGCAACATGG AGAGTGCCGT GGGCTCCCTC TACATCAAGC GGGCCTTCTC CAAGGACAGC 
AAGAGCATAG TCAGTGAGCT TATCGAGAAG ATACGGTCCG TGTTTGTGGA TAACCTGGAC 
GAGTTGAACT GGATGGATGA GGAATCCAAG AAAAAGGCCC AGGAAAAGGC CTTGAATATC 
CGGGAACAGA TCGGCTACCC TGACTACATT TTGGAAGACA ATAACAGACA CCTGGATGAG 
GAATACTCCA GTCTGACTTT CTCAGAGGAC CTGTATTTTG AGAACGGGCT TCAGAACCTC 
AAGAACAATG CCCAAAGGAG CCTCAAGAAA CTTCGGGAAA AGGTGGACCA GAATCTCTGG 
ATCATTGGGG CTGCAGTGGT CAATGCATTC TACTCCCCAA ACAGAAACCT GATCGTCTTT 
CCAGCGGGGA TCCTCCAGCC ACCCTTCTTC AGCAAGGACC AACCACAGGC CTTGAATTTC 
GGGGGCATCG GGATGGTGAT TGGACACGAG ATCACACACG GCTTTGATGA TAACGGTCGG 
AACTTTGACA AGAATGGCAA CATGCTGGAC TGGTGGAGCA ACTTCTCGGC CCGGCACTTC 
CGACAGCAGT CACAGTGTAT GATTTATCAG TACAGCAACT TCTCTTGGGA ACTAGCAGAC 
AACCAGAATG TGAACGGATT CAGCACCCTC GGGGAGAACA TCGCCGACAA CGGCGGTGTG 
CGGCAGGCAT ACAAGGCTTA CCTACAGTGG CTAGCTGAAG GCGGCAGAGA CCAGAGACTG 
CCGGGACTGA ACCTGACCTA TGCTCAGCTT TTCTTCATTA ACTATGCCCA GGTGTGGTGT 
GGGTCCTACA GGCCGGAGTT CGCCATCCAG TCCATCAAGA CAGATGTCCA CAGTCCTCTT 
AAGTACAGGG TGCTGGGCTC ACTACAGAAC CTACCAGGCT TCTCTGAGGC GTTCCACTGC 
CCACGAGGCA GCCCCATGCA CCCTATGAAT CGATGTCGCA TCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101467.1
CDS	176..2503
Translation	MGKSESSVGMMERADNCGRRRLGFVECGLLVLLTLLLMGAIVTLGVFYSIGKQLPLLNSLLHVSRHERTVVKRAALRDSSQKSDICTTPSCVIAAARILQNMDQSKKPCDNFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSSVQHRPAVEKAKTLYRSCMNQSVIEKRDSEPLLNVLDMIGGWPVAMDKWNETMGPKWELERQLAVLNSQFNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFKEDSHRVREAYLQFMTSVATMLRRDLNLPGETDLVQEEMAQVLHLETHLANATVPQEKRHDVTALYHRMGLEELQERFGLKGFNWTLFIQNVLSSVQVELLPNEEVVVYGIPYLENLEEIIDVFPAQTLQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTMEEVRWRECVSYVNSNMESAVGSLYIKRAFSKDSKSIVSELIEKIRSVFVDNLDELNWMDEESKKKAQEKALNIREQIGYPDYILEDNNRHLDEEYSSLTFSEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGAAVVNAFYSPNRNLIVFPAGILQPPFFSKDQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARHFRQQSQCMIYQYSNFSWELADNQNVNGFSTLGENIADNGGVRQAYKAYLQWLAEGGRDQRLPGLNLTYAQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLPGFSEAFHCPRGSPMHPMNRCRIW

Target ORF information:

RefSeq Version	NM_001107997.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107997.1

ORF Insert Sequence:

ATGGGGAAGT CGGAGAGCTC AGTGGGGATG ATGGAGAGAG CGGACAACTG TGGGAGGAGG 
CGCCTAGGCT TCGTGGAGTG TGGGCTGCTG GTACTGCTGA CACTGCTGTT GATGGGAGCC 
ATAGTGACTC TGGGTGTCTT CTACAGCATA GGGAAGCAGC TGCCCCTCTT AAATAGCCTG 
CTGCACGTCT CCCGGCATGA GAGGACGGTT GTAAAACGAG CCGCCCTCAG AGATTCATCG 
CAGAAGAGTG ACATCTGTAC TACCCCAAGC TGCGTGATAG CAGCTGCCAG AATCCTCCAG 
AACATGGACC AGTCAAAGAA ACCCTGCGAC AACTTCTATC AGTATGCTTG CGGAGGCTGG 
CTACGGCACC ATGTGATCCC CGAGACCAAC TCCAGATACA GCGTCTTTGA CATCCTTCGG 
GATGAGCTGG AGGTCATCCT CAAAGGGGTG CTGGAGGATT CCTCTGTCCA GCACCGCCCA 
GCTGTGGAGA AGGCCAAGAC ACTGTACCGC TCCTGCATGA ACCAGAGTGT GATAGAGAAG 
AGAGACTCTG AGCCCCTGCT GAACGTCTTA GATATGATAG GAGGTTGGCC TGTAGCCATG 
GACAAGTGGA ATGAGACCAT GGGCCCCAAG TGGGAACTGG AGCGGCAGTT GGCTGTGTTG 
AACTCGCAGT TCAACAGGCG CGTCCTCATC GACCTCTTCA TCTGGAATGA TGACCAGAAC 
TCCAGCCGGC ACGTCATCTA CATAGACCAG CCCACCTTGG GCATGCCCTC CCGGGAGTAC 
TATTTCAAGG AAGACAGCCA CCGGGTACGG GAAGCCTACC TGCAGTTCAT GACATCAGTG 
GCCACTATGC TGAGGAGAGA CCTGAACCTG CCCGGGGAGA CCGATTTGGT GCAGGAGGAA 
ATGGCACAGG TGCTGCATCT GGAGACACAT CTGGCCAACG CCACGGTCCC CCAGGAGAAA 
AGGCATGATG TCACCGCCCT GTATCACCGA ATGGGCCTGG AGGAGCTGCA GGAAAGGTTT 
GGTCTGAAGG GGTTTAACTG GACTCTCTTC ATACAAAACG TGCTGTCTTC TGTGCAAGTT 
GAGCTGCTCC CGAATGAGGA GGTGGTGGTC TATGGCATCC CCTACCTGGA GAATCTTGAG 
GAGATCATTG ACGTCTTCCC AGCACAGACC TTGCAAAACT ACCTGGTGTG GCGCCTGGTG 
CTAGATCGCA TCGGCAGCCT GAGCCAGAGA TTCAAAGAAG CGCGTGTGGA CTACCGCAAG 
GCGCTGTACG GTACAACCAT GGAGGAAGTA CGCTGGCGGG AGTGTGTCAG CTATGTCAAC 
AGCAACATGG AGAGTGCCGT GGGCTCCCTC TACATCAAGC GGGCCTTCTC CAAGGACAGC 
AAGAGCATAG TCAGTGAGCT TATCGAGAAG ATACGGTCCG TGTTTGTGGA TAACCTGGAC 
GAGTTGAACT GGATGGATGA GGAATCCAAG AAAAAGGCCC AGGAAAAGGC CTTGAATATC 
CGGGAACAGA TCGGCTACCC TGACTACATT TTGGAAGACA ATAACAGACA CCTGGATGAG 
GAATACTCCA GTCTGACTTT CTCAGAGGAC CTGTATTTTG AGAACGGGCT TCAGAACCTC 
AAGAACAATG CCCAAAGGAG CCTCAAGAAA CTTCGGGAAA AGGTGGACCA GAATCTCTGG 
ATCATTGGGG CTGCAGTGGT CAATGCATTC TACTCCCCAA ACAGAAACCT GATCGTCTTT 
CCAGCGGGGA TCCTCCAGCC ACCCTTCTTC AGCAAGGACC AACCACAGGC CTTGAATTTC 
GGGGGCATCG GGATGGTGAT TGGACACGAG ATCACACACG GCTTTGATGA TAACGGTCGG 
AACTTTGACA AGAATGGCAA CATGCTGGAC TGGTGGAGCA ACTTCTCGGC CCGGCACTTC 
CGACAGCAGT CACAGTGTAT GATTTATCAG TACAGCAACT TCTCTTGGGA ACTAGCAGAC 
AACCAGAATG TGAACGGATT CAGCACCCTC GGGGAGAACA TCGCCGACAA CGGCGGTGTG 
CGGCAGGCAT ACAAGGCTTA CCTACAGTGG CTAGCTGAAG GCGGCAGAGA CCAGAGACTG 
CCGGGACTGA ACCTGACCTA TGCTCAGCTT TTCTTCATTA ACTATGCCCA GGTGTGGTGT 
GGGTCCTACA GGCCGGAGTT CGCCATCCAG TCCATCAAGA CAGATGTCCA CAGTCCTCTT 
AAGTACAGGG TGCTGGGCTC ACTACAGAAC CTACCAGGCT TCTCTGAGGC GTTCCACTGC 
CCACGAGGCA GCCCCATGCA CCCTATGAAT CGATGTCGCA TCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mmel1 ( XM_006239567.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239629.1 Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29004
Clone ID Related Accession (Same CDS sequence)	XM_006239567.3
Accession Version	XM_006239567.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane metallo-endopeptidase-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239567.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGT CGGAGAGCTC AGTGGGGATG ATGGAGAGAG CGGACAACTG TGGGAGGAGG 
CGCCTAGGCT TCGTGGAGTG TGGGCTGCTG GTACTGCTGA CACTGCTGTT GATGGGAGCC 
ATAGTGACTC TGGGTGTCTT CTACAGCATA GGGAAGCAGC TGCCCCTCTT AAATAGCCTG 
CTGCACGTCT CCCGGCATGA GAGGACGGTT GTAAAACGAG TCCTCAGAGA TTCATCGCAG 
AAGAGTGACA TCTGTACTAC CCCAAGCTGC GTGATAGCAG CTGCCAGAAT CCTCCAGAAC 
ATGGACCAGT CAAAGAAACC CTGCGACAAC TTCTATCAGT ATGCTTGCGG AGGCTGGCTA 
CGGCACCATG TGATCCCCGA GACCAACTCC AGATACAGCG TCTTTGACAT CCTTCGGGAT 
GAGCTGGAGG TCATCCTCAA AGGGGTGCTG GAGGATTCCT CTGTCCAGCA CCGCCCAGCT 
GTGGAGAAGG CCAAGACACT GTACCGCTCC TGCATGAACC AGAGTGTGAT AGAGAAGAGA 
GACTCTGAGC CCCTGCTGAA CGTCTTAGAT ATGATAGGAG GTTGGCCTGT AGCCATGGAC 
AAGTGGAATG AGACCATGGG CCCCAAGTGG GAACTGGAGC GGCAGTTGGC TGTGTTGAAC 
TCGCAGTTCA ACAGGCGCGT CCTCATCGAC CTCTTCATCT GGAATGATGA CCAGAACTCC 
AGCCGGCACG TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCCTCCCG GGAGTACTAT 
TTCAAGGAAG ACAGCCACCG GGTACGGGAA GCCTACCTGC AGTTCATGAC ATCAGTGGCC 
ACTATGCTGA GGAGAGACCT GAACCTGCCC GGGGAGACCG ATTTGGTGCA GGAGGAAATG 
GCACAGGTGC TGCATCTGGA GACACATCTG GCCAACGCCA CGGTCCCCCA GGAGAAAAGG 
CATGATGTCA CCGCCCTGTA TCACCGAATG GGCCTGGAGG AGCTGCAGGA AAGGTTTGGT 
CTGAAGGGGT TTAACTGGAC TCTCTTCATA CAAAACGTGC TGTCTTCTGT GCAAGTTGAG 
CTGCTCCCGA ATGAGGAGGT GGTGGTCTAT GGCATCCCCT ACCTGGAGAA TCTTGAGGAG 
ATCATTGACG TCTTCCCAGC ACAGACCTTG CAAAACTACC TGGTGTGGCG CCTGGTGCTA 
GATCGCATCG GCAGCCTGAG CCAGAGATTC AAAGAAGCGC GTGTGGACTA CCGCAAGGCG 
CTGTACGGTA CAACCATGGA GGAAGTACGC TGGCGGGAGT GTGTCAGCTA TGTCAACAGC 
AACATGGAGA GTGCCGTGGG CTCCCTCTAC ATCAAGCGGG CCTTCTCCAA GGACAGCAAG 
AGCATAGTCA GTGAGCTTAT CGAGAAGATA CGGTCCGTGT TTGTGGATAA CCTGGACGAG 
TTGAACTGGA TGGATGAGGA ATCCAAGAAA AAGGCCCAGG AAAAGGCCTT GAATATCCGG 
GAACAGATCG GCTACCCTGA CTACATTTTG GAAGACAATA ACAGACACCT GGATGAGGAA 
TACTCCAGTC TGACTTTCTC AGAGGACCTG TATTTTGAGA ACGGGCTTCA GAACCTCAAG 
AACAATGCCC AAAGGAGCCT CAAGAAACTT CGGGAAAAGG TGGACCAGAA TCTCTGGATC 
ATTGGGGCTG CAGTGGTCAA TGCATTCTAC TCCCCAAACA GAAACCTGAT CGTCTTTCCA 
GCGGGGATCC TCCAGCCACC CTTCTTCAGC AAGGACCAAC CACAGGCCTT GAATTTCGGG 
GGCATCGGGA TGGTGATTGG ACACGAGATC ACACACGGCT TTGATGATAA CGGTCGGAAC 
TTTGACAAGA ATGGCAACAT GCTGGACTGG TGGAGCAACT TCTCGGCCCG GCACTTCCGA 
CAGCAGTCAC AGTGTATGAT TTATCAGTAC AGCAACTTCT CTTGGGAACT AGCAGACAAC 
CAGAATGTGA ACGGATTCAG CACCCTCGGG GAGAACATCG CCGACAACGG CGGTGTGCGG 
CAGGCATACA AGGCTTACCT ACAGTGGCTA GCTGAAGGCG GCAGAGACCA GAGACTGCCG 
GGACTGAACC TGACCTATGC TCAGCTTTTC TTCATTAACT ATGCCCAGGT GTGGTGTGGG 
TCCTACAGGC CGGAGTTCGC CATCCAGTCC ATCAAGACAG ATGTCCACAG TCCTCTTAAG 
TACAGGGTGC TGGGCTCACT ACAGAACCTA CCAGGCTTCT CTGAGGCGTT CCACTGCCCA 
CGAGGCAGCC CCATGCACCC TATGAATCGA TGTCGCATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239629.1
CDS	174..2498
Translation	MGKSESSVGMMERADNCGRRRLGFVECGLLVLLTLLLMGAIVTLGVFYSIGKQLPLLNSLLHVSRHERTVVKRVLRDSSQKSDICTTPSCVIAAARILQNMDQSKKPCDNFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSSVQHRPAVEKAKTLYRSCMNQSVIEKRDSEPLLNVLDMIGGWPVAMDKWNETMGPKWELERQLAVLNSQFNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFKEDSHRVREAYLQFMTSVATMLRRDLNLPGETDLVQEEMAQVLHLETHLANATVPQEKRHDVTALYHRMGLEELQERFGLKGFNWTLFIQNVLSSVQVELLPNEEVVVYGIPYLENLEEIIDVFPAQTLQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTMEEVRWRECVSYVNSNMESAVGSLYIKRAFSKDSKSIVSELIEKIRSVFVDNLDELNWMDEESKKKAQEKALNIREQIGYPDYILEDNNRHLDEEYSSLTFSEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGAAVVNAFYSPNRNLIVFPAGILQPPFFSKDQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARHFRQQSQCMIYQYSNFSWELADNQNVNGFSTLGENIADNGGVRQAYKAYLQWLAEGGRDQRLPGLNLTYAQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLPGFSEAFHCPRGSPMHPMNRCRIW

Target ORF information:

RefSeq Version	XM_006239567.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239567.3

ORF Insert Sequence:

ATGGGGAAGT CGGAGAGCTC AGTGGGGATG ATGGAGAGAG CGGACAACTG TGGGAGGAGG 
CGCCTAGGCT TCGTGGAGTG TGGGCTGCTG GTACTGCTGA CACTGCTGTT GATGGGAGCC 
ATAGTGACTC TGGGTGTCTT CTACAGCATA GGGAAGCAGC TGCCCCTCTT AAATAGCCTG 
CTGCACGTCT CCCGGCATGA GAGGACGGTT GTAAAACGAG TCCTCAGAGA TTCATCGCAG 
AAGAGTGACA TCTGTACTAC CCCAAGCTGC GTGATAGCAG CTGCCAGAAT CCTCCAGAAC 
ATGGACCAGT CAAAGAAACC CTGCGACAAC TTCTATCAGT ATGCTTGCGG AGGCTGGCTA 
CGGCACCATG TGATCCCCGA GACCAACTCC AGATACAGCG TCTTTGACAT CCTTCGGGAT 
GAGCTGGAGG TCATCCTCAA AGGGGTGCTG GAGGATTCCT CTGTCCAGCA CCGCCCAGCT 
GTGGAGAAGG CCAAGACACT GTACCGCTCC TGCATGAACC AGAGTGTGAT AGAGAAGAGA 
GACTCTGAGC CCCTGCTGAA CGTCTTAGAT ATGATAGGAG GTTGGCCTGT AGCCATGGAC 
AAGTGGAATG AGACCATGGG CCCCAAGTGG GAACTGGAGC GGCAGTTGGC TGTGTTGAAC 
TCGCAGTTCA ACAGGCGCGT CCTCATCGAC CTCTTCATCT GGAATGATGA CCAGAACTCC 
AGCCGGCACG TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCCTCCCG GGAGTACTAT 
TTCAAGGAAG ACAGCCACCG GGTACGGGAA GCCTACCTGC AGTTCATGAC ATCAGTGGCC 
ACTATGCTGA GGAGAGACCT GAACCTGCCC GGGGAGACCG ATTTGGTGCA GGAGGAAATG 
GCACAGGTGC TGCATCTGGA GACACATCTG GCCAACGCCA CGGTCCCCCA GGAGAAAAGG 
CATGATGTCA CCGCCCTGTA TCACCGAATG GGCCTGGAGG AGCTGCAGGA AAGGTTTGGT 
CTGAAGGGGT TTAACTGGAC TCTCTTCATA CAAAACGTGC TGTCTTCTGT GCAAGTTGAG 
CTGCTCCCGA ATGAGGAGGT GGTGGTCTAT GGCATCCCCT ACCTGGAGAA TCTTGAGGAG 
ATCATTGACG TCTTCCCAGC ACAGACCTTG CAAAACTACC TGGTGTGGCG CCTGGTGCTA 
GATCGCATCG GCAGCCTGAG CCAGAGATTC AAAGAAGCGC GTGTGGACTA CCGCAAGGCG 
CTGTACGGTA CAACCATGGA GGAAGTACGC TGGCGGGAGT GTGTCAGCTA TGTCAACAGC 
AACATGGAGA GTGCCGTGGG CTCCCTCTAC ATCAAGCGGG CCTTCTCCAA GGACAGCAAG 
AGCATAGTCA GTGAGCTTAT CGAGAAGATA CGGTCCGTGT TTGTGGATAA CCTGGACGAG 
TTGAACTGGA TGGATGAGGA ATCCAAGAAA AAGGCCCAGG AAAAGGCCTT GAATATCCGG 
GAACAGATCG GCTACCCTGA CTACATTTTG GAAGACAATA ACAGACACCT GGATGAGGAA 
TACTCCAGTC TGACTTTCTC AGAGGACCTG TATTTTGAGA ACGGGCTTCA GAACCTCAAG 
AACAATGCCC AAAGGAGCCT CAAGAAACTT CGGGAAAAGG TGGACCAGAA TCTCTGGATC 
ATTGGGGCTG CAGTGGTCAA TGCATTCTAC TCCCCAAACA GAAACCTGAT CGTCTTTCCA 
GCGGGGATCC TCCAGCCACC CTTCTTCAGC AAGGACCAAC CACAGGCCTT GAATTTCGGG 
GGCATCGGGA TGGTGATTGG ACACGAGATC ACACACGGCT TTGATGATAA CGGTCGGAAC 
TTTGACAAGA ATGGCAACAT GCTGGACTGG TGGAGCAACT TCTCGGCCCG GCACTTCCGA 
CAGCAGTCAC AGTGTATGAT TTATCAGTAC AGCAACTTCT CTTGGGAACT AGCAGACAAC 
CAGAATGTGA ACGGATTCAG CACCCTCGGG GAGAACATCG CCGACAACGG CGGTGTGCGG 
CAGGCATACA AGGCTTACCT ACAGTGGCTA GCTGAAGGCG GCAGAGACCA GAGACTGCCG 
GGACTGAACC TGACCTATGC TCAGCTTTTC TTCATTAACT ATGCCCAGGT GTGGTGTGGG 
TCCTACAGGC CGGAGTTCGC CATCCAGTCC ATCAAGACAG ATGTCCACAG TCCTCTTAAG 
TACAGGGTGC TGGGCTCACT ACAGAACCTA CCAGGCTTCT CTGAGGCGTT CCACTGCCCA 
CGAGGCAGCC CCATGCACCC TATGAATCGA TGTCGCATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mmel1 ( XM_006239569.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239631.1 Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa29006
Clone ID Related Accession (Same CDS sequence)	XM_006239569.3
Accession Version	XM_006239569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane metallo-endopeptidase-like 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGT CGGAGAGCTC AGTGGGGATG ATGGAGAGAG CGGACAACTG TGGGAGGAGG 
CGCCTAGGCT TCGTGGAGTG TGGGCTGCTG GTACTGCTGA CACTGCTGTT GATGGGAGCC 
ATAGTGACTC TGGGTGTCTT CTACAGCATA GGGAAGCAGC TGCCCCTCTT AAATAGCCTG 
CTGCACGTCT CCCGGCATGA GAGGACGGTT GTAAAACGAG TCCTCAGAGA TTCATCGCAG 
AAGAGTGACA TCTGTACTAC CCCAAGCTGC GTGATAGCAG CTGCCAGAAT CCTCCAGAAC 
ATGGACCAGT CAAAGAAACC CTGCGACAAC TTCTATCAGT ATGCTTGCGG AGGCTGGCTA 
CGGCACCATG TGATCCCCGA GACCAACTCC AGATACAGCG TCTTTGACAT CCTTCGGGAT 
GAGCTGGAGG TCATCCTCAA AGGGGTGCTG GAGGATTCCT CTGTCCAGCA CCGCCCAGCT 
GTGGAGAAGG CCAAGACACT GTACCGCTCC TGCATGAACC AGAGTGTGAT AGAGAAGAGA 
GACTCTGAGC CCCTGCTGAA CGTCTTAGAT ATGATAGGAG GTTGGCCTGT AGCCATGGAC 
AAGTGGAATG AGACCATGGG CCCCAAGTGG GAACTGGAGC GGCAGTTGGC TGTGTTGAAC 
TCGCAGTTCA ACAGGCGCGT CCTCATCGAC CTCTTCATCT GGAATGATGA CCAGAACTCC 
AGCCGGCACG TCATCTACGT ACGGGAAGCC TACCTGCAGT TCATGACATC AGTGGCCACT 
ATGCTGAGGA GAGACCTGAA CCTGCCCGGG GAGACCGATT TGGTGCAGGA GGAAATGGCA 
CAGGTGCTGC ATCTGGAGAC ACATCTGGCC AACGCCACGG TCCCCCAGGA GAAAAGGCAT 
GATGTCACCG CCCTGTATCA CCGAATGGGC CTGGAGGAGC TGCAGGAAAG GTTTGGTCTG 
AAGGGGTTTA ACTGGACTCT CTTCATACAA AACGTGCTGT CTTCTGTGCA AGTTGAGCTG 
CTCCCGAATG AGGAGGTGGT GGTCTATGGC ATCCCCTACC TGGAGAATCT TGAGGAGATC 
ATTGACGTCT TCCCAGCACA GACCTTGCAA AACTACCTGG TGTGGCGCCT GGTGCTAGAT 
CGCATCGGCA GCCTGAGCCA GAGATTCAAA GAAGCGCGTG TGGACTACCG CAAGGCGCTG 
TACGGTACAA CCATGGAGGA AGTACGCTGG CGGGAGTGTG TCAGCTATGT CAACAGCAAC 
ATGGAGAGTG CCGTGGGCTC CCTCTACATC AAGCGGGCCT TCTCCAAGGA CAGCAAGAGC 
ATAGTCAGTG AGCTTATCGA GAAGATACGG TCCGTGTTTG TGGATAACCT GGACGAGTTG 
AACTGGATGG ATGAGGAATC CAAGAAAAAG GCCCAGGAAA AGGCCTTGAA TATCCGGGAA 
CAGATCGGCT ACCCTGACTA CATTTTGGAA GACAATAACA GACACCTGGA TGAGGAATAC 
TCCAGTCTGA CTTTCTCAGA GGACCTGTAT TTTGAGAACG GGCTTCAGAA CCTCAAGAAC 
AATGCCCAAA GGAGCCTCAA GAAACTTCGG GAAAAGGTGG ACCAGAATCT CTGGATCATT 
GGGGCTGCAG TGGTCAATGC ATTCTACTCC CCAAACAGAA ACCTGATCGT CTTTCCAGCG 
GGGATCCTCC AGCCACCCTT CTTCAGCAAG GACCAACCAC AGGCCTTGAA TTTCGGGGGC 
ATCGGGATGG TGATTGGACA CGAGATCACA CACGGCTTTG ATGATAACGG TCGGAACTTT 
GACAAGAATG GCAACATGCT GGACTGGTGG AGCAACTTCT CGGCCCGGCA CTTCCGACAG 
CAGTCACAGT GTATGATTTA TCAGTACAGC AACTTCTCTT GGGAACTAGC AGACAACCAG 
AATGTGAACG GATTCAGCAC CCTCGGGGAG AACATCGCCG ACAACGGCGG TGTGCGGCAG 
GCATACAAGG CTTACCTACA GTGGCTAGCT GAAGGCGGCA GAGACCAGAG ACTGCCGGGA 
CTGAACCTGA CCTATGCTCA GCTTTTCTTC ATTAACTATG CCCAGGTGTG GTGTGGGTCC 
TACAGGCCGG AGTTCGCCAT CCAGTCCATC AAGACAGATG TCCACAGTCC TCTTAAGTAC 
AGGGTGCTGG GCTCACTACA GAACCTACCA GGCTTCTCTG AGGCGTTCCA CTGCCCACGA 
GGCAGCCCCA TGCACCCTAT GAATCGATGT CGCATCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239631.1
CDS	174..2435
Translation	MGKSESSVGMMERADNCGRRRLGFVECGLLVLLTLLLMGAIVTLGVFYSIGKQLPLLNSLLHVSRHERTVVKRVLRDSSQKSDICTTPSCVIAAARILQNMDQSKKPCDNFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSSVQHRPAVEKAKTLYRSCMNQSVIEKRDSEPLLNVLDMIGGWPVAMDKWNETMGPKWELERQLAVLNSQFNRRVLIDLFIWNDDQNSSRHVIYVREAYLQFMTSVATMLRRDLNLPGETDLVQEEMAQVLHLETHLANATVPQEKRHDVTALYHRMGLEELQERFGLKGFNWTLFIQNVLSSVQVELLPNEEVVVYGIPYLENLEEIIDVFPAQTLQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTMEEVRWRECVSYVNSNMESAVGSLYIKRAFSKDSKSIVSELIEKIRSVFVDNLDELNWMDEESKKKAQEKALNIREQIGYPDYILEDNNRHLDEEYSSLTFSEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGAAVVNAFYSPNRNLIVFPAGILQPPFFSKDQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARHFRQQSQCMIYQYSNFSWELADNQNVNGFSTLGENIADNGGVRQAYKAYLQWLAEGGRDQRLPGLNLTYAQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLPGFSEAFHCPRGSPMHPMNRCRIW

Target ORF information:

RefSeq Version	XM_006239569.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239569.3

ORF Insert Sequence:

ATGGGGAAGT CGGAGAGCTC AGTGGGGATG ATGGAGAGAG CGGACAACTG TGGGAGGAGG 
CGCCTAGGCT TCGTGGAGTG TGGGCTGCTG GTACTGCTGA CACTGCTGTT GATGGGAGCC 
ATAGTGACTC TGGGTGTCTT CTACAGCATA GGGAAGCAGC TGCCCCTCTT AAATAGCCTG 
CTGCACGTCT CCCGGCATGA GAGGACGGTT GTAAAACGAG TCCTCAGAGA TTCATCGCAG 
AAGAGTGACA TCTGTACTAC CCCAAGCTGC GTGATAGCAG CTGCCAGAAT CCTCCAGAAC 
ATGGACCAGT CAAAGAAACC CTGCGACAAC TTCTATCAGT ATGCTTGCGG AGGCTGGCTA 
CGGCACCATG TGATCCCCGA GACCAACTCC AGATACAGCG TCTTTGACAT CCTTCGGGAT 
GAGCTGGAGG TCATCCTCAA AGGGGTGCTG GAGGATTCCT CTGTCCAGCA CCGCCCAGCT 
GTGGAGAAGG CCAAGACACT GTACCGCTCC TGCATGAACC AGAGTGTGAT AGAGAAGAGA 
GACTCTGAGC CCCTGCTGAA CGTCTTAGAT ATGATAGGAG GTTGGCCTGT AGCCATGGAC 
AAGTGGAATG AGACCATGGG CCCCAAGTGG GAACTGGAGC GGCAGTTGGC TGTGTTGAAC 
TCGCAGTTCA ACAGGCGCGT CCTCATCGAC CTCTTCATCT GGAATGATGA CCAGAACTCC 
AGCCGGCACG TCATCTACGT ACGGGAAGCC TACCTGCAGT TCATGACATC AGTGGCCACT 
ATGCTGAGGA GAGACCTGAA CCTGCCCGGG GAGACCGATT TGGTGCAGGA GGAAATGGCA 
CAGGTGCTGC ATCTGGAGAC ACATCTGGCC AACGCCACGG TCCCCCAGGA GAAAAGGCAT 
GATGTCACCG CCCTGTATCA CCGAATGGGC CTGGAGGAGC TGCAGGAAAG GTTTGGTCTG 
AAGGGGTTTA ACTGGACTCT CTTCATACAA AACGTGCTGT CTTCTGTGCA AGTTGAGCTG 
CTCCCGAATG AGGAGGTGGT GGTCTATGGC ATCCCCTACC TGGAGAATCT TGAGGAGATC 
ATTGACGTCT TCCCAGCACA GACCTTGCAA AACTACCTGG TGTGGCGCCT GGTGCTAGAT 
CGCATCGGCA GCCTGAGCCA GAGATTCAAA GAAGCGCGTG TGGACTACCG CAAGGCGCTG 
TACGGTACAA CCATGGAGGA AGTACGCTGG CGGGAGTGTG TCAGCTATGT CAACAGCAAC 
ATGGAGAGTG CCGTGGGCTC CCTCTACATC AAGCGGGCCT TCTCCAAGGA CAGCAAGAGC 
ATAGTCAGTG AGCTTATCGA GAAGATACGG TCCGTGTTTG TGGATAACCT GGACGAGTTG 
AACTGGATGG ATGAGGAATC CAAGAAAAAG GCCCAGGAAA AGGCCTTGAA TATCCGGGAA 
CAGATCGGCT ACCCTGACTA CATTTTGGAA GACAATAACA GACACCTGGA TGAGGAATAC 
TCCAGTCTGA CTTTCTCAGA GGACCTGTAT TTTGAGAACG GGCTTCAGAA CCTCAAGAAC 
AATGCCCAAA GGAGCCTCAA GAAACTTCGG GAAAAGGTGG ACCAGAATCT CTGGATCATT 
GGGGCTGCAG TGGTCAATGC ATTCTACTCC CCAAACAGAA ACCTGATCGT CTTTCCAGCG 
GGGATCCTCC AGCCACCCTT CTTCAGCAAG GACCAACCAC AGGCCTTGAA TTTCGGGGGC 
ATCGGGATGG TGATTGGACA CGAGATCACA CACGGCTTTG ATGATAACGG TCGGAACTTT 
GACAAGAATG GCAACATGCT GGACTGGTGG AGCAACTTCT CGGCCCGGCA CTTCCGACAG 
CAGTCACAGT GTATGATTTA TCAGTACAGC AACTTCTCTT GGGAACTAGC AGACAACCAG 
AATGTGAACG GATTCAGCAC CCTCGGGGAG AACATCGCCG ACAACGGCGG TGTGCGGCAG 
GCATACAAGG CTTACCTACA GTGGCTAGCT GAAGGCGGCA GAGACCAGAG ACTGCCGGGA 
CTGAACCTGA CCTATGCTCA GCTTTTCTTC ATTAACTATG CCCAGGTGTG GTGTGGGTCC 
TACAGGCCGG AGTTCGCCAT CCAGTCCATC AAGACAGATG TCCACAGTCC TCTTAAGTAC 
AGGGTGCTGG GCTCACTACA GAACCTACCA GGCTTCTCTG AGGCGTTCCA CTGCCCACGA 
GGCAGCCCCA TGCACCCTAT GAATCGATGT CGCATCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mmel1 ( XM_006239570.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239632.1 Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa29007
Clone ID Related Accession (Same CDS sequence)	XM_006239570.3
Accession Version	XM_006239570.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane metallo-endopeptidase-like 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239570.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGT CGGAGAGCTC AGTGGGGATG ATGGAGAGAG CGGACAACTG TGGGAGGAGG 
CGCCTAGGCT TCGTGGAGTG TGGGCTGCTG GTACTGCTGA CACTGCTGTT GATGGGAGCC 
ATAGTGACTC TGGGTGTCTT CTACAGCATA GTCCTCAGAG ATTCATCGCA GAAGAGTGAC 
ATCTGTACTA CCCCAAGCTG CGTGATAGCA GCTGCCAGAA TCCTCCAGAA CATGGACCAG 
TCAAAGAAAC CCTGCGACAA CTTCTATCAG TATGCTTGCG GAGGCTGGCT ACGGCACCAT 
GTGATCCCCG AGACCAACTC CAGATACAGC GTCTTTGACA TCCTTCGGGA TGAGCTGGAG 
GTCATCCTCA AAGGGGTGCT GGAGGATTCC TCTGTCCAGC ACCGCCCAGC TGTGGAGAAG 
GCCAAGACAC TGTACCGCTC CTGCATGAAC CAGAGTGTGA TAGAGAAGAG AGACTCTGAG 
CCCCTGCTGA ACGTCTTAGA TATGATAGGA GGTTGGCCTG TAGCCATGGA CAAGTGGAAT 
GAGACCATGG GCCCCAAGTG GGAACTGGAG CGGCAGTTGG CTGTGTTGAA CTCGCAGTTC 
AACAGGCGCG TCCTCATCGA CCTCTTCATC TGGAATGATG ACCAGAACTC CAGCCGGCAC 
GTCATCTACA TAGACCAGCC CACCTTGGGC ATGCCCTCCC GGGAGTACTA TTTCAAGGAA 
GACAGCCACC GGGTACGGGA AGCCTACCTG CAGTTCATGA CATCAGTGGC CACTATGCTG 
AGGAGAGACC TGAACCTGCC CGGGGAGACC GATTTGGTGC AGGAGGAAAT GGCACAGGTG 
CTGCATCTGG AGACACATCT GGCCAACGCC ACGGTCCCCC AGGAGAAAAG GCATGATGTC 
ACCGCCCTGT ATCACCGAAT GGGCCTGGAG GAGCTGCAGG AAAGGTTTGG TCTGAAGGGG 
TTTAACTGGA CTCTCTTCAT ACAAAACGTG CTGTCTTCTG TGCAAGTTGA GCTGCTCCCG 
AATGAGGAGG TGGTGGTCTA TGGCATCCCC TACCTGGAGA ATCTTGAGGA GATCATTGAC 
GTCTTCCCAG CACAGACCTT GCAAAACTAC CTGGTGTGGC GCCTGGTGCT AGATCGCATC 
GGCAGCCTGA GCCAGAGATT CAAAGAAGCG CGTGTGGACT ACCGCAAGGC GCTGTACGGT 
ACAACCATGG AGGAAGTACG CTGGCGGGAG TGTGTCAGCT ATGTCAACAG CAACATGGAG 
AGTGCCGTGG GCTCCCTCTA CATCAAGCGG GCCTTCTCCA AGGACAGCAA GAGCATAGTC 
AGTGAGCTTA TCGAGAAGAT ACGGTCCGTG TTTGTGGATA ACCTGGACGA GTTGAACTGG 
ATGGATGAGG AATCCAAGAA AAAGGCCCAG GAAAAGGCCT TGAATATCCG GGAACAGATC 
GGCTACCCTG ACTACATTTT GGAAGACAAT AACAGACACC TGGATGAGGA ATACTCCAGT 
CTGACTTTCT CAGAGGACCT GTATTTTGAG AACGGGCTTC AGAACCTCAA GAACAATGCC 
CAAAGGAGCC TCAAGAAACT TCGGGAAAAG GTGGACCAGA ATCTCTGGAT CATTGGGGCT 
GCAGTGGTCA ATGCATTCTA CTCCCCAAAC AGAAACCTGA TCGTCTTTCC AGCGGGGATC 
CTCCAGCCAC CCTTCTTCAG CAAGGACCAA CCACAGGCCT TGAATTTCGG GGGCATCGGG 
ATGGTGATTG GACACGAGAT CACACACGGC TTTGATGATA ACGGTCGGAA CTTTGACAAG 
AATGGCAACA TGCTGGACTG GTGGAGCAAC TTCTCGGCCC GGCACTTCCG ACAGCAGTCA 
CAGTGTATGA TTTATCAGTA CAGCAACTTC TCTTGGGAAC TAGCAGACAA CCAGAATGTG 
AACGGATTCA GCACCCTCGG GGAGAACATC GCCGACAACG GCGGTGTGCG GCAGGCATAC 
AAGGCTTACC TACAGTGGCT AGCTGAAGGC GGCAGAGACC AGAGACTGCC GGGACTGAAC 
CTGACCTATG CTCAGCTTTT CTTCATTAAC TATGCCCAGG TGTGGTGTGG GTCCTACAGG 
CCGGAGTTCG CCATCCAGTC CATCAAGACA GATGTCCACA GTCCTCTTAA GTACAGGGTG 
CTGGGCTCAC TACAGAACCT ACCAGGCTTC TCTGAGGCGT TCCACTGCCC ACGAGGCAGC 
CCCATGCACC CTATGAATCG ATGTCGCATC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239632.1
CDS	174..2429
Translation	MGKSESSVGMMERADNCGRRRLGFVECGLLVLLTLLLMGAIVTLGVFYSIVLRDSSQKSDICTTPSCVIAAARILQNMDQSKKPCDNFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSSVQHRPAVEKAKTLYRSCMNQSVIEKRDSEPLLNVLDMIGGWPVAMDKWNETMGPKWELERQLAVLNSQFNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFKEDSHRVREAYLQFMTSVATMLRRDLNLPGETDLVQEEMAQVLHLETHLANATVPQEKRHDVTALYHRMGLEELQERFGLKGFNWTLFIQNVLSSVQVELLPNEEVVVYGIPYLENLEEIIDVFPAQTLQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTMEEVRWRECVSYVNSNMESAVGSLYIKRAFSKDSKSIVSELIEKIRSVFVDNLDELNWMDEESKKKAQEKALNIREQIGYPDYILEDNNRHLDEEYSSLTFSEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGAAVVNAFYSPNRNLIVFPAGILQPPFFSKDQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARHFRQQSQCMIYQYSNFSWELADNQNVNGFSTLGENIADNGGVRQAYKAYLQWLAEGGRDQRLPGLNLTYAQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLPGFSEAFHCPRGSPMHPMNRCRIW

Target ORF information:

RefSeq Version	XM_006239570.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239570.3

ORF Insert Sequence:

ATGGGGAAGT CGGAGAGCTC AGTGGGGATG ATGGAGAGAG CGGACAACTG TGGGAGGAGG 
CGCCTAGGCT TCGTGGAGTG TGGGCTGCTG GTACTGCTGA CACTGCTGTT GATGGGAGCC 
ATAGTGACTC TGGGTGTCTT CTACAGCATA GTCCTCAGAG ATTCATCGCA GAAGAGTGAC 
ATCTGTACTA CCCCAAGCTG CGTGATAGCA GCTGCCAGAA TCCTCCAGAA CATGGACCAG 
TCAAAGAAAC CCTGCGACAA CTTCTATCAG TATGCTTGCG GAGGCTGGCT ACGGCACCAT 
GTGATCCCCG AGACCAACTC CAGATACAGC GTCTTTGACA TCCTTCGGGA TGAGCTGGAG 
GTCATCCTCA AAGGGGTGCT GGAGGATTCC TCTGTCCAGC ACCGCCCAGC TGTGGAGAAG 
GCCAAGACAC TGTACCGCTC CTGCATGAAC CAGAGTGTGA TAGAGAAGAG AGACTCTGAG 
CCCCTGCTGA ACGTCTTAGA TATGATAGGA GGTTGGCCTG TAGCCATGGA CAAGTGGAAT 
GAGACCATGG GCCCCAAGTG GGAACTGGAG CGGCAGTTGG CTGTGTTGAA CTCGCAGTTC 
AACAGGCGCG TCCTCATCGA CCTCTTCATC TGGAATGATG ACCAGAACTC CAGCCGGCAC 
GTCATCTACA TAGACCAGCC CACCTTGGGC ATGCCCTCCC GGGAGTACTA TTTCAAGGAA 
GACAGCCACC GGGTACGGGA AGCCTACCTG CAGTTCATGA CATCAGTGGC CACTATGCTG 
AGGAGAGACC TGAACCTGCC CGGGGAGACC GATTTGGTGC AGGAGGAAAT GGCACAGGTG 
CTGCATCTGG AGACACATCT GGCCAACGCC ACGGTCCCCC AGGAGAAAAG GCATGATGTC 
ACCGCCCTGT ATCACCGAAT GGGCCTGGAG GAGCTGCAGG AAAGGTTTGG TCTGAAGGGG 
TTTAACTGGA CTCTCTTCAT ACAAAACGTG CTGTCTTCTG TGCAAGTTGA GCTGCTCCCG 
AATGAGGAGG TGGTGGTCTA TGGCATCCCC TACCTGGAGA ATCTTGAGGA GATCATTGAC 
GTCTTCCCAG CACAGACCTT GCAAAACTAC CTGGTGTGGC GCCTGGTGCT AGATCGCATC 
GGCAGCCTGA GCCAGAGATT CAAAGAAGCG CGTGTGGACT ACCGCAAGGC GCTGTACGGT 
ACAACCATGG AGGAAGTACG CTGGCGGGAG TGTGTCAGCT ATGTCAACAG CAACATGGAG 
AGTGCCGTGG GCTCCCTCTA CATCAAGCGG GCCTTCTCCA AGGACAGCAA GAGCATAGTC 
AGTGAGCTTA TCGAGAAGAT ACGGTCCGTG TTTGTGGATA ACCTGGACGA GTTGAACTGG 
ATGGATGAGG AATCCAAGAA AAAGGCCCAG GAAAAGGCCT TGAATATCCG GGAACAGATC 
GGCTACCCTG ACTACATTTT GGAAGACAAT AACAGACACC TGGATGAGGA ATACTCCAGT 
CTGACTTTCT CAGAGGACCT GTATTTTGAG AACGGGCTTC AGAACCTCAA GAACAATGCC 
CAAAGGAGCC TCAAGAAACT TCGGGAAAAG GTGGACCAGA ATCTCTGGAT CATTGGGGCT 
GCAGTGGTCA ATGCATTCTA CTCCCCAAAC AGAAACCTGA TCGTCTTTCC AGCGGGGATC 
CTCCAGCCAC CCTTCTTCAG CAAGGACCAA CCACAGGCCT TGAATTTCGG GGGCATCGGG 
ATGGTGATTG GACACGAGAT CACACACGGC TTTGATGATA ACGGTCGGAA CTTTGACAAG 
AATGGCAACA TGCTGGACTG GTGGAGCAAC TTCTCGGCCC GGCACTTCCG ACAGCAGTCA 
CAGTGTATGA TTTATCAGTA CAGCAACTTC TCTTGGGAAC TAGCAGACAA CCAGAATGTG 
AACGGATTCA GCACCCTCGG GGAGAACATC GCCGACAACG GCGGTGTGCG GCAGGCATAC 
AAGGCTTACC TACAGTGGCT AGCTGAAGGC GGCAGAGACC AGAGACTGCC GGGACTGAAC 
CTGACCTATG CTCAGCTTTT CTTCATTAAC TATGCCCAGG TGTGGTGTGG GTCCTACAGG 
CCGGAGTTCG CCATCCAGTC CATCAAGACA GATGTCCACA GTCCTCTTAA GTACAGGGTG 
CTGGGCTCAC TACAGAACCT ACCAGGCTTC TCTGAGGCGT TCCACTGCCC ACGAGGCAGC 
CCCATGCACC CTATGAATCG ATGTCGCATC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mmel1 ( XM_008764360.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762582.1 Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa47264
Clone ID Related Accession (Same CDS sequence)	XM_008764360.2
Accession Version	XM_008764360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane metallo-endopeptidase-like 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGT CAAAGAAACC CTGCGACAAC TTCTATCAGT ATGCTTGCGG AGGCTGGCTA 
CGGCACCATG TGATCCCCGA GACCAACTCC AGATACAGCG TCTTTGACAT CCTTCGGGAT 
GAGCTGGAGG TCATCCTCAA AGGGGTGCTG GAGGATTCCT CTGTCCAGCA CCGCCCAGCT 
GTGGAGAAGG CCAAGACACT GTACCGCTCC TGCATGAACC AGAGTGTGAT AGAGAAGAGA 
GACTCTGAGC CCCTGCTGAA CGTCTTAGAT ATGATAGGAG GTTGGCCTGT AGCCATGGAC 
AAGTGGAATG AGACCATGGG CCCCAAGTGG GAACTGGAGC GGCAGTTGGC TGTGTTGAAC 
TCGCAGTTCA ACAGGCGCGT CCTCATCGAC CTCTTCATCT GGAATGATGA CCAGAACTCC 
AGCCGGCACG TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCCTCCCG GGAGTACTAT 
TTCAAGGAAG ACAGCCACCG GGTACGGGAA GCCTACCTGC AGTTCATGAC ATCAGTGGCC 
ACTATGCTGA GGAGAGACCT GAACCTGCCC GGGGAGACCG ATTTGGTGCA GGAGGAAATG 
GCACAGGTGC TGCATCTGGA GACACATCTG GCCAACGCCA CGGTCCCCCA GGAGAAAAGG 
CATGATGTCA CCGCCCTGTA TCACCGAATG GGCCTGGAGG AGCTGCAGGA AAGGTTTGGT 
CTGAAGGGGT TTAACTGGAC TCTCTTCATA CAAAACGTGC TGTCTTCTGT GCAAGTTGAG 
CTGCTCCCGA ATGAGGAGGT GGTGGTCTAT GGCATCCCCT ACCTGGAGAA TCTTGAGGAG 
ATCATTGACG TCTTCCCAGC ACAGACCTTG CAAAACTACC TGGTGTGGCG CCTGGTGCTA 
GATCGCATCG GCAGCCTGAG CCAGAGATTC AAAGAAGCGC GTGTGGACTA CCGCAAGGCG 
CTGTACGGTA CAACCATGGA GGAAGTACGC TGGCGGGAGT GTGTCAGCTA TGTCAACAGC 
AACATGGAGA GTGCCGTGGG CTCCCTCTAC ATCAAGCGGG CCTTCTCCAA GGACAGCAAG 
AGCATAGTCA GTGAGCTTAT CGAGAAGATA CGGTCCGTGT TTGTGGATAA CCTGGACGAG 
TTGAACTGGA TGGATGAGGA ATCCAAGAAA AAGGCCCAGG AAAAGGCCTT GAATATCCGG 
GAACAGATCG GCTACCCTGA CTACATTTTG GAAGACAATA ACAGACACCT GGATGAGGAA 
TACTCCAGTC TGACTTTCTC AGAGGACCTG TATTTTGAGA ACGGGCTTCA GAACCTCAAG 
AACAATGCCC AAAGGAGCCT CAAGAAACTT CGGGAAAAGG TGGACCAGAA TCTCTGGATC 
ATTGGGGCTG CAGTGGTCAA TGCATTCTAC TCCCCAAACA GAAACCTGAT CGTCTTTCCA 
GCGGGGATCC TCCAGCCACC CTTCTTCAGC AAGGACCAAC CACAGGCCTT GAATTTCGGG 
GGCATCGGGA TGGTGATTGG ACACGAGATC ACACACGGCT TTGATGATAA CGGTCGGAAC 
TTTGACAAGA ATGGCAACAT GCTGGACTGG TGGAGCAACT TCTCGGCCCG GCACTTCCGA 
CAGCAGTCAC AGTGTATGAT TTATCAGTAC AGCAACTTCT CTTGGGAACT AGCAGACAAC 
CAGAATGTGA ACGGATTCAG CACCCTCGGG GAGAACATCG CCGACAACGG CGGTGTGCGG 
CAGGCATACA AGGCTTACCT ACAGTGGCTA GCTGAAGGCG GCAGAGACCA GAGACTGCCG 
GGACTGAACC TGACCTATGC TCAGCTTTTC TTCATTAACT ATGCCCAGGT GTGGTGTGGG 
TCCTACAGGC CGGAGTTCGC CATCCAGTCC ATCAAGACAG ATGTCCACAG TCCTCTTAAG 
TACAGGGTGC TGGGCTCACT ACAGAACCTA CCAGGCTTCT CTGAGGCGTT CCACTGCCCA 
CGAGGCAGCC CCATGCACCC TATGAATCGA TGTCGCATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762582.1
CDS	118..2142
Translation	MDQSKKPCDNFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSSVQHRPAVEKAKTLYRSCMNQSVIEKRDSEPLLNVLDMIGGWPVAMDKWNETMGPKWELERQLAVLNSQFNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFKEDSHRVREAYLQFMTSVATMLRRDLNLPGETDLVQEEMAQVLHLETHLANATVPQEKRHDVTALYHRMGLEELQERFGLKGFNWTLFIQNVLSSVQVELLPNEEVVVYGIPYLENLEEIIDVFPAQTLQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTMEEVRWRECVSYVNSNMESAVGSLYIKRAFSKDSKSIVSELIEKIRSVFVDNLDELNWMDEESKKKAQEKALNIREQIGYPDYILEDNNRHLDEEYSSLTFSEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGAAVVNAFYSPNRNLIVFPAGILQPPFFSKDQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARHFRQQSQCMIYQYSNFSWELADNQNVNGFSTLGENIADNGGVRQAYKAYLQWLAEGGRDQRLPGLNLTYAQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLPGFSEAFHCPRGSPMHPMNRCRIW

Target ORF information:

RefSeq Version	XM_008764360.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764360.2

ORF Insert Sequence:

ATGGACCAGT CAAAGAAACC CTGCGACAAC TTCTATCAGT ATGCTTGCGG AGGCTGGCTA 
CGGCACCATG TGATCCCCGA GACCAACTCC AGATACAGCG TCTTTGACAT CCTTCGGGAT 
GAGCTGGAGG TCATCCTCAA AGGGGTGCTG GAGGATTCCT CTGTCCAGCA CCGCCCAGCT 
GTGGAGAAGG CCAAGACACT GTACCGCTCC TGCATGAACC AGAGTGTGAT AGAGAAGAGA 
GACTCTGAGC CCCTGCTGAA CGTCTTAGAT ATGATAGGAG GTTGGCCTGT AGCCATGGAC 
AAGTGGAATG AGACCATGGG CCCCAAGTGG GAACTGGAGC GGCAGTTGGC TGTGTTGAAC 
TCGCAGTTCA ACAGGCGCGT CCTCATCGAC CTCTTCATCT GGAATGATGA CCAGAACTCC 
AGCCGGCACG TCATCTACAT AGACCAGCCC ACCTTGGGCA TGCCCTCCCG GGAGTACTAT 
TTCAAGGAAG ACAGCCACCG GGTACGGGAA GCCTACCTGC AGTTCATGAC ATCAGTGGCC 
ACTATGCTGA GGAGAGACCT GAACCTGCCC GGGGAGACCG ATTTGGTGCA GGAGGAAATG 
GCACAGGTGC TGCATCTGGA GACACATCTG GCCAACGCCA CGGTCCCCCA GGAGAAAAGG 
CATGATGTCA CCGCCCTGTA TCACCGAATG GGCCTGGAGG AGCTGCAGGA AAGGTTTGGT 
CTGAAGGGGT TTAACTGGAC TCTCTTCATA CAAAACGTGC TGTCTTCTGT GCAAGTTGAG 
CTGCTCCCGA ATGAGGAGGT GGTGGTCTAT GGCATCCCCT ACCTGGAGAA TCTTGAGGAG 
ATCATTGACG TCTTCCCAGC ACAGACCTTG CAAAACTACC TGGTGTGGCG CCTGGTGCTA 
GATCGCATCG GCAGCCTGAG CCAGAGATTC AAAGAAGCGC GTGTGGACTA CCGCAAGGCG 
CTGTACGGTA CAACCATGGA GGAAGTACGC TGGCGGGAGT GTGTCAGCTA TGTCAACAGC 
AACATGGAGA GTGCCGTGGG CTCCCTCTAC ATCAAGCGGG CCTTCTCCAA GGACAGCAAG 
AGCATAGTCA GTGAGCTTAT CGAGAAGATA CGGTCCGTGT TTGTGGATAA CCTGGACGAG 
TTGAACTGGA TGGATGAGGA ATCCAAGAAA AAGGCCCAGG AAAAGGCCTT GAATATCCGG 
GAACAGATCG GCTACCCTGA CTACATTTTG GAAGACAATA ACAGACACCT GGATGAGGAA 
TACTCCAGTC TGACTTTCTC AGAGGACCTG TATTTTGAGA ACGGGCTTCA GAACCTCAAG 
AACAATGCCC AAAGGAGCCT CAAGAAACTT CGGGAAAAGG TGGACCAGAA TCTCTGGATC 
ATTGGGGCTG CAGTGGTCAA TGCATTCTAC TCCCCAAACA GAAACCTGAT CGTCTTTCCA 
GCGGGGATCC TCCAGCCACC CTTCTTCAGC AAGGACCAAC CACAGGCCTT GAATTTCGGG 
GGCATCGGGA TGGTGATTGG ACACGAGATC ACACACGGCT TTGATGATAA CGGTCGGAAC 
TTTGACAAGA ATGGCAACAT GCTGGACTGG TGGAGCAACT TCTCGGCCCG GCACTTCCGA 
CAGCAGTCAC AGTGTATGAT TTATCAGTAC AGCAACTTCT CTTGGGAACT AGCAGACAAC 
CAGAATGTGA ACGGATTCAG CACCCTCGGG GAGAACATCG CCGACAACGG CGGTGTGCGG 
CAGGCATACA AGGCTTACCT ACAGTGGCTA GCTGAAGGCG GCAGAGACCA GAGACTGCCG 
GGACTGAACC TGACCTATGC TCAGCTTTTC TTCATTAACT ATGCCCAGGT GTGGTGTGGG 
TCCTACAGGC CGGAGTTCGC CATCCAGTCC ATCAAGACAG ATGTCCACAG TCCTCTTAAG 
TACAGGGTGC TGGGCTCACT ACAGAACCTA CCAGGCTTCT CTGAGGCGTT CCACTGCCCA 
CGAGGCAGCC CCATGCACCC TATGAATCGA TGTCGCATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human neprilysin-2 (NEP2) and NEP display distinct subcellular localisations and substrate preferences.
FEBS letters582(16)2382-6(2008 Jul)
Whyteside AR,Turner AJ

The ultimate tryptophan residue of neprilysin 2 is not involved in protein maturation and enzymatic activity.
Biochemical and biophysical research communications335(2)356-60(2005 Sep)
Voisin S,Ouimet T

A three-dimensional model of the neprilysin 2 active site based on the X-ray structure of neprilysin. Identification of residues involved in substrate hydrolysis and inhibitor binding of neprilysin 2.
The Journal of biological chemistry279(44)46172-81(2004 Oct)
Voisin S,Rognan D,Gros C,Ouimet T

Ontogeny, regional and cellular distribution of the novel metalloprotease neprilysin 2 in the rat: a comparison with neprilysin and endothelin-converting enzyme-1.
Neuroscience118(3)627-39(2003)
Facchinetti P,Rose C,Schwartz JC,Ouimet T

Cell-specific activity of neprilysin 2 isoforms and enzymic specificity compared with neprilysin.
The Biochemical journal363(Pt 3)697-705(2002 May)
Rose C,Voisin S,Gros C,Schwartz JC,Ouimet T

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Mmel1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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