Gfi1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gfi1b
Entrez Gene ID	311832
Full Name	growth factor independent 1B transcriptional repressor
General protein information	Preferred Names growth factor independent 1B transcriptional repressor Names zinc finger protein Gfi-1b growth factor independent 1B transcription repressor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12

mRNA and Protein(s)

mRNA	Protein	Name
XM_008761647.2	XP_008759869.1	zinc finger protein Gfi-1b isoform X1
XM_008761651.1	XP_008759873.1	zinc finger protein Gfi-1b isoform X3
XM_008761646.1	XP_008759868.1	zinc finger protein Gfi-1b isoform X1
XM_006233789.1	XP_006233851.1	zinc finger protein Gfi-1b isoform X2
XM_008761650.2	XP_008759872.1	zinc finger protein Gfi-1b isoform X1
NM_001107823.1	NP_001101293.1	zinc finger protein Gfi-1b
XM_008761649.1	XP_008759871.1	zinc finger protein Gfi-1b isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gfi1b	XP_002936223.1
Pan troglodytes (chimpanzee)	GFI1B	XP_528450.2
Bos taurus (cattle)	GFI1B	NP_001179849.1
Mus musculus (house mouse)	Gfi1b	NP_032140.1
Danio rerio (zebrafish)	gfi1b	NP_001258770.1
Gallus gallus (chicken)	GFI1B	NP_990422.1
Homo sapiens (human)	GFI1B	NP_004179.3
	GFI1B	XP_001103109.2
Canis lupus familiaris (dog)	GFI1B	XP_005625226.1
Rattus norvegicus (Norway rat)	Gfi1b	NP_001101293.1

Gene Ontology
Bibliography

Related articles in PubMed

A dominant-negative GFI1B mutation in the gray platelet syndrome.
Monteferrario D, Bolar NA, Marneth AE, Hebeda KM, Bergevoet SM, Veenstra H, Laros-van Gorkom BA, MacKenzie MA, Khandanpour C, Botezatu L, Fransen E, Van Camp G, Duijnhouwer AL, Salemink S, Willemsen B, Huls G, Preijers F, Van Heerde W, Jansen JH, Kempers MJ, Loeys BL, Van Laer L, Van der Reijden BA
The New England journal of medicine370(3)245-53(2014 Jan)

Growth factor independent 1b (Gfi1b) and a new splice variant of Gfi1b are highly expressed in patients with acute and chronic leukemia.
Vassen L, Khandanpour C, Ebeling P, van der Reijden BA, Jansen JH, Mahlmann S, D?hrsen U, M?r?y T
International journal of hematology89(4)422-30(2009 May)

Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1.
Saleque S, Kim J, Rooke HM, Orkin SH
Molecular cell27(4)562-72(2007 Aug)

Growth factor-independent 1B gene (GFI1B) is overexpressed in erythropoietic and megakaryocytic malignancies and increases their proliferation rate.
Elmaagacli AH, Koldehoff M, Zakrzewski JL, Steckel NK, Ottinger H, Beelen DW
British journal of haematology136(2)212-9(2007 Jan)

GATA-1 forms distinct activating and repressive complexes in erythroid cells.
Rodriguez P, Bonte E, Krijgsveld J, Kolodziej KE, Guyot B, Heck AJ, Vyas P, de Boer E, Grosveld F, Strouboulis J
The EMBO journal24(13)2354-66(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gfi1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gfi1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06064	NM_001107823.1 Latest version!	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa25586	XM_006233789.1 Latest version!	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa47074	XM_008761646.1 Latest version!	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa47074	XM_008761647.2 Latest version!	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa47074	XM_008761650.2 Latest version!	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa47074	XM_008761649.1 Latest version!	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa47075	XM_008761651.1 Latest version!	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gfi1b ( NM_001107823.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101293.1 Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), mRNA.

Price & Availability↑

CloneID	ORa06064
Clone ID Related Accession (Same CDS sequence)	NM_001107823.1
Accession Version	NM_001107823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474001.2. On or before Oct 4, 2007 this sequence version replaced XM_231109.3, XM_001079155.1. ##Evidence-Data-START## Transcript exon combination :: BC158685.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689594, SAMEA2689599 [ECO:0000350] ##Evidence-Data-END##

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG TGGCAAAAGA ACATACCCAG 
AGTGGCAGCC CTGTTCTCAG CACACTGCTT CCAAGCCAGA CCTTGGACTG GAGCGCAGTC 
AAACAGAAGC AGGAGATGCT GCTGAACCAG AGCCTTCCCA AGATGGCCTC AGCCCCAGAG 
GGGCCTCACG TGACATCCCA ACCCCAGGAT GGGGAATCGC CACTCTCCGA GTCACCCCCT 
TTCTATAAGC CCAGCTTCTC TTGGGATACG CTGGCCTCCT CCTACAGACA CAGCTACCAA 
CAGACCCCCT CCACCATGCA GTCAGCTTTC CTGGAGCGCT CCGTGAGGCT GTACGGCAGC 
CCGCTTGTGC CCAGCACCGA GTCCCCCTTG GACTTCAGCC TCCGTTATTC TCCGGGCATG 
GACACTTACC ACTGTGTCAA GTGTAACAAG GTGTTCTCCA CCCCTCATGG GCTAGAAGTG 
CATGTCCGCC GCTCTCACAG CGGAACCCGG CCCTTTGCCT GTGACATCTG TGGCAAAACC 
TTTGGCCACG CTGTGAGTTT GGAGCAGCAC ACGCACGTGC ATTCCCAGGA GCGTAGCTTC 
GAGTGCCGGA TGTGTGGCAA AGCCTTCAAG CGCTCATCCA CTCTGTCCAC CCACCTGCTC 
ATCCATTCGG ACACTCGGCC CTACCCCTGC CAGTTCTGTG GGAAACGCTT CCACCAGAAG 
TCGGACATGA AGAAACACAC CTACATCCAC ACAGGTGAGA AGCCTCACAA GTGCCAGGTG 
TGTGGAAAAG CCTTCAGCCA AAGCTCCAAC CTCATCACCC ACAGCCGCAA GCACACAGGC 
TTCAAGCCCT TCAGCTGTGA GCTGTGCACA AAGGGCTTCC AGCGCAAGGT GGACCTGCGA 
CGTCACCGTG AGAGCCAACA TAACCTCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101293.1
CDS	120..1112
Translation	MPRSFLVKSKKAHTYHQPRAQGDDLVWPPAVIPVAKEHTQSGSPVLSTLLPSQTLDWSAVKQKQEMLLNQSLPKMASAPEGPHVTSQPQDGESPLSESPPFYKPSFSWDTLASSYRHSYQQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFSLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDICGKTFGHAVSLEQHTHVHSQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK

Target ORF information:

RefSeq Version	NM_001107823.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107823.1

ORF Insert Sequence:

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG TGGCAAAAGA ACATACCCAG 
AGTGGCAGCC CTGTTCTCAG CACACTGCTT CCAAGCCAGA CCTTGGACTG GAGCGCAGTC 
AAACAGAAGC AGGAGATGCT GCTGAACCAG AGCCTTCCCA AGATGGCCTC AGCCCCAGAG 
GGGCCTCACG TGACATCCCA ACCCCAGGAT GGGGAATCGC CACTCTCCGA GTCACCCCCT 
TTCTATAAGC CCAGCTTCTC TTGGGATACG CTGGCCTCCT CCTACAGACA CAGCTACCAA 
CAGACCCCCT CCACCATGCA GTCAGCTTTC CTGGAGCGCT CCGTGAGGCT GTACGGCAGC 
CCGCTTGTGC CCAGCACCGA GTCCCCCTTG GACTTCAGCC TCCGTTATTC TCCGGGCATG 
GACACTTACC ACTGTGTCAA GTGTAACAAG GTGTTCTCCA CCCCTCATGG GCTAGAAGTG 
CATGTCCGCC GCTCTCACAG CGGAACCCGG CCCTTTGCCT GTGACATCTG TGGCAAAACC 
TTTGGCCACG CTGTGAGTTT GGAGCAGCAC ACGCACGTGC ATTCCCAGGA GCGTAGCTTC 
GAGTGCCGGA TGTGTGGCAA AGCCTTCAAG CGCTCATCCA CTCTGTCCAC CCACCTGCTC 
ATCCATTCGG ACACTCGGCC CTACCCCTGC CAGTTCTGTG GGAAACGCTT CCACCAGAAG 
TCGGACATGA AGAAACACAC CTACATCCAC ACAGGTGAGA AGCCTCACAA GTGCCAGGTG 
TGTGGAAAAG CCTTCAGCCA AAGCTCCAAC CTCATCACCC ACAGCCGCAA GCACACAGGC 
TTCAAGCCCT TCAGCTGTGA GCTGTGCACA AAGGGCTTCC AGCGCAAGGT GGACCTGCGA 
CGTCACCGTG AGAGCCAACA TAACCTCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1b ( XM_006233789.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233851.1 Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa25586
Clone ID Related Accession (Same CDS sequence)	XM_006233789.1
Accession Version	XM_006233789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG TGGCAAAAGA ACATACCCAG 
AGTGGCAGCC CTGTTCTCAG CACACTGCTT CCAAGCCAGA CCTTGGACTG GAGCGCAGTC 
AAACAGAAGC AGGAGATGCT GCTGAACCAG AGCCTTCCCA AGATGGCCTC AGCCCCAGAG 
GGGCCTCACG TGACATCCCA ACCCCAGGAT GGGGAATCGC CACTCTCCGA GTCACCCCCT 
TTCTATAAGC CCAGCTTCTC TTGGGATACG CTGGCCTCCT CCTACAGACA CAGCTACCAA 
CAGACCCCCT CCACCATGCA GTCAGCTTTC CTGGAGCGCT CCGTGAGGCT GTACGGCAGC 
CCGCTTGTGC CCAGCACCGA GTCCCCCTTG GACTTCAGCC TCCGTTATTC TCCGGGCATG 
GACACTTACC ACTGTGTCAA GTGTAACAAG GTGTTCTCCA CCCCTCATGG GCTAGAAGTG 
CATGTCCGCC GCTCTCACAG CGGAACCCGG CCCTTTGCCT GTGACATCTG TGGCAAAACC 
TTTGGCCACG CTGTGAGTTT GGAGCAGCAC ACGCACGTGC ATTCCCAGGG TGTCCCAGCC 
AGGTCCAGTC CCTCGCCCAG CTTGGCTGTC CCGGGCCTCG AGGCCCCACC TGCACCTGAC 
CCCCCAAGGC CTCGTTTCCT CCGGCAGGAG CGTAGCTTCG AGTGCCGGAT GTGTGGCAAA 
GCCTTCAAGC GCTCATCCAC TCTGTCCACC CACCTGCTCA TCCATTCGGA CACTCGGCCC 
TACCCCTGCC AGTTCTGTGG GAAACGCTTC CACCAGAAGT CGGACATGAA GAAACACACC 
TACATCCACA CAGGTGAGAA GCCTCACAAG TGCCAGGTGT GTGGAAAAGC CTTCAGCCAA 
AGCTCCAACC TCATCACCCA CAGCCGCAAG CACACAGGCT TCAAGCCCTT CAGCTGTGAG 
CTGTGCACAA AGGGCTTCCA GCGCAAGGTG GACCTGCGAC GTCACCGTGA GAGCCAACAT 
AACCTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233851.1
CDS	159..1250
Translation	MPRSFLVKSKKAHTYHQPRAQGDDLVWPPAVIPVAKEHTQSGSPVLSTLLPSQTLDWSAVKQKQEMLLNQSLPKMASAPEGPHVTSQPQDGESPLSESPPFYKPSFSWDTLASSYRHSYQQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFSLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDICGKTFGHAVSLEQHTHVHSQGVPARSSPSPSLAVPGLEAPPAPDPPRPRFLRQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK

Target ORF information:

RefSeq Version	XM_006233789.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233789.1

ORF Insert Sequence:

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG TGGCAAAAGA ACATACCCAG 
AGTGGCAGCC CTGTTCTCAG CACACTGCTT CCAAGCCAGA CCTTGGACTG GAGCGCAGTC 
AAACAGAAGC AGGAGATGCT GCTGAACCAG AGCCTTCCCA AGATGGCCTC AGCCCCAGAG 
GGGCCTCACG TGACATCCCA ACCCCAGGAT GGGGAATCGC CACTCTCCGA GTCACCCCCT 
TTCTATAAGC CCAGCTTCTC TTGGGATACG CTGGCCTCCT CCTACAGACA CAGCTACCAA 
CAGACCCCCT CCACCATGCA GTCAGCTTTC CTGGAGCGCT CCGTGAGGCT GTACGGCAGC 
CCGCTTGTGC CCAGCACCGA GTCCCCCTTG GACTTCAGCC TCCGTTATTC TCCGGGCATG 
GACACTTACC ACTGTGTCAA GTGTAACAAG GTGTTCTCCA CCCCTCATGG GCTAGAAGTG 
CATGTCCGCC GCTCTCACAG CGGAACCCGG CCCTTTGCCT GTGACATCTG TGGCAAAACC 
TTTGGCCACG CTGTGAGTTT GGAGCAGCAC ACGCACGTGC ATTCCCAGGG TGTCCCAGCC 
AGGTCCAGTC CCTCGCCCAG CTTGGCTGTC CCGGGCCTCG AGGCCCCACC TGCACCTGAC 
CCCCCAAGGC CTCGTTTCCT CCGGCAGGAG CGTAGCTTCG AGTGCCGGAT GTGTGGCAAA 
GCCTTCAAGC GCTCATCCAC TCTGTCCACC CACCTGCTCA TCCATTCGGA CACTCGGCCC 
TACCCCTGCC AGTTCTGTGG GAAACGCTTC CACCAGAAGT CGGACATGAA GAAACACACC 
TACATCCACA CAGGTGAGAA GCCTCACAAG TGCCAGGTGT GTGGAAAAGC CTTCAGCCAA 
AGCTCCAACC TCATCACCCA CAGCCGCAAG CACACAGGCT TCAAGCCCTT CAGCTGTGAG 
CTGTGCACAA AGGGCTTCCA GCGCAAGGTG GACCTGCGAC GTCACCGTGA GAGCCAACAT 
AACCTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1b ( XM_008761647.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759869.1 Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa47074
Clone ID Related Accession (Same CDS sequence)	XM_008761647.2 , XM_008761646.1 , XM_008761650.2 , XM_008761649.1
Accession Version	XM_008761647.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGGTG TCCCAGCCAG GTCCAGTCCC 
TCGCCCAGCT TGGCTGTCCC GGGCCTCGAG GCCCCACCTG CACCTGACCC CCCAAGGCCT 
CGTTTCCTCC GGCAGGAGCG TAGCTTCGAG TGCCGGATGT GTGGCAAAGC CTTCAAGCGC 
TCATCCACTC TGTCCACCCA CCTGCTCATC CATTCGGACA CTCGGCCCTA CCCCTGCCAG 
TTCTGTGGGA AACGCTTCCA CCAGAAGTCG GACATGAAGA AACACACCTA CATCCACACA 
GGTGAGAAGC CTCACAAGTG CCAGGTGTGT GGAAAAGCCT TCAGCCAAAG CTCCAACCTC 
ATCACCCACA GCCGCAAGCA CACAGGCTTC AAGCCCTTCA GCTGTGAGCT GTGCACAAAG 
GGCTTCCAGC GCAAGGTGGA CCTGCGACGT CACCGTGAGA GCCAACATAA CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759869.1
CDS	3431..4570
Translation	MPRSFLVKSKKAHTYHQPRAQGDDLVWPPAVIPGIPELMVVTLGSYLLTVAKEHTQSGSPVLSTLLPSQTLDWSAVKQKQEMLLNQSLPKMASAPEGPHVTSQPQDGESPLSESPPFYKPSFSWDTLASSYRHSYQQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFSLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDICGKTFGHAVSLEQHTHVHSQGVPARSSPSPSLAVPGLEAPPAPDPPRPRFLRQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK

Target ORF information:

RefSeq Version	XM_008761647.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761647.2

ORF Insert Sequence:

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGGTG TCCCAGCCAG GTCCAGTCCC 
TCGCCCAGCT TGGCTGTCCC GGGCCTCGAG GCCCCACCTG CACCTGACCC CCCAAGGCCT 
CGTTTCCTCC GGCAGGAGCG TAGCTTCGAG TGCCGGATGT GTGGCAAAGC CTTCAAGCGC 
TCATCCACTC TGTCCACCCA CCTGCTCATC CATTCGGACA CTCGGCCCTA CCCCTGCCAG 
TTCTGTGGGA AACGCTTCCA CCAGAAGTCG GACATGAAGA AACACACCTA CATCCACACA 
GGTGAGAAGC CTCACAAGTG CCAGGTGTGT GGAAAAGCCT TCAGCCAAAG CTCCAACCTC 
ATCACCCACA GCCGCAAGCA CACAGGCTTC AAGCCCTTCA GCTGTGAGCT GTGCACAAAG 
GGCTTCCAGC GCAAGGTGGA CCTGCGACGT CACCGTGAGA GCCAACATAA CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1b ( XM_008761646.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759868.1 Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47074
Clone ID Related Accession (Same CDS sequence)	XM_008761647.2 , XM_008761646.1 , XM_008761650.2 , XM_008761649.1
Accession Version	XM_008761646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGGTG TCCCAGCCAG GTCCAGTCCC 
TCGCCCAGCT TGGCTGTCCC GGGCCTCGAG GCCCCACCTG CACCTGACCC CCCAAGGCCT 
CGTTTCCTCC GGCAGGAGCG TAGCTTCGAG TGCCGGATGT GTGGCAAAGC CTTCAAGCGC 
TCATCCACTC TGTCCACCCA CCTGCTCATC CATTCGGACA CTCGGCCCTA CCCCTGCCAG 
TTCTGTGGGA AACGCTTCCA CCAGAAGTCG GACATGAAGA AACACACCTA CATCCACACA 
GGTGAGAAGC CTCACAAGTG CCAGGTGTGT GGAAAAGCCT TCAGCCAAAG CTCCAACCTC 
ATCACCCACA GCCGCAAGCA CACAGGCTTC AAGCCCTTCA GCTGTGAGCT GTGCACAAAG 
GGCTTCCAGC GCAAGGTGGA CCTGCGACGT CACCGTGAGA GCCAACATAA CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759868.1
CDS	159..1298
Translation	MPRSFLVKSKKAHTYHQPRAQGDDLVWPPAVIPGIPELMVVTLGSYLLTVAKEHTQSGSPVLSTLLPSQTLDWSAVKQKQEMLLNQSLPKMASAPEGPHVTSQPQDGESPLSESPPFYKPSFSWDTLASSYRHSYQQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFSLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDICGKTFGHAVSLEQHTHVHSQGVPARSSPSPSLAVPGLEAPPAPDPPRPRFLRQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK

Target ORF information:

RefSeq Version	XM_008761646.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761646.1

ORF Insert Sequence:

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGGTG TCCCAGCCAG GTCCAGTCCC 
TCGCCCAGCT TGGCTGTCCC GGGCCTCGAG GCCCCACCTG CACCTGACCC CCCAAGGCCT 
CGTTTCCTCC GGCAGGAGCG TAGCTTCGAG TGCCGGATGT GTGGCAAAGC CTTCAAGCGC 
TCATCCACTC TGTCCACCCA CCTGCTCATC CATTCGGACA CTCGGCCCTA CCCCTGCCAG 
TTCTGTGGGA AACGCTTCCA CCAGAAGTCG GACATGAAGA AACACACCTA CATCCACACA 
GGTGAGAAGC CTCACAAGTG CCAGGTGTGT GGAAAAGCCT TCAGCCAAAG CTCCAACCTC 
ATCACCCACA GCCGCAAGCA CACAGGCTTC AAGCCCTTCA GCTGTGAGCT GTGCACAAAG 
GGCTTCCAGC GCAAGGTGGA CCTGCGACGT CACCGTGAGA GCCAACATAA CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1b ( XM_008761650.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759872.1 Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa47074
Clone ID Related Accession (Same CDS sequence)	XM_008761647.2 , XM_008761646.1 , XM_008761650.2 , XM_008761649.1
Accession Version	XM_008761650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGGTG TCCCAGCCAG GTCCAGTCCC 
TCGCCCAGCT TGGCTGTCCC GGGCCTCGAG GCCCCACCTG CACCTGACCC CCCAAGGCCT 
CGTTTCCTCC GGCAGGAGCG TAGCTTCGAG TGCCGGATGT GTGGCAAAGC CTTCAAGCGC 
TCATCCACTC TGTCCACCCA CCTGCTCATC CATTCGGACA CTCGGCCCTA CCCCTGCCAG 
TTCTGTGGGA AACGCTTCCA CCAGAAGTCG GACATGAAGA AACACACCTA CATCCACACA 
GGTGAGAAGC CTCACAAGTG CCAGGTGTGT GGAAAAGCCT TCAGCCAAAG CTCCAACCTC 
ATCACCCACA GCCGCAAGCA CACAGGCTTC AAGCCCTTCA GCTGTGAGCT GTGCACAAAG 
GGCTTCCAGC GCAAGGTGGA CCTGCGACGT CACCGTGAGA GCCAACATAA CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759872.1
CDS	714..1853
Translation	MPRSFLVKSKKAHTYHQPRAQGDDLVWPPAVIPGIPELMVVTLGSYLLTVAKEHTQSGSPVLSTLLPSQTLDWSAVKQKQEMLLNQSLPKMASAPEGPHVTSQPQDGESPLSESPPFYKPSFSWDTLASSYRHSYQQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFSLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDICGKTFGHAVSLEQHTHVHSQGVPARSSPSPSLAVPGLEAPPAPDPPRPRFLRQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK

Target ORF information:

RefSeq Version	XM_008761650.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761650.2

ORF Insert Sequence:

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGGTG TCCCAGCCAG GTCCAGTCCC 
TCGCCCAGCT TGGCTGTCCC GGGCCTCGAG GCCCCACCTG CACCTGACCC CCCAAGGCCT 
CGTTTCCTCC GGCAGGAGCG TAGCTTCGAG TGCCGGATGT GTGGCAAAGC CTTCAAGCGC 
TCATCCACTC TGTCCACCCA CCTGCTCATC CATTCGGACA CTCGGCCCTA CCCCTGCCAG 
TTCTGTGGGA AACGCTTCCA CCAGAAGTCG GACATGAAGA AACACACCTA CATCCACACA 
GGTGAGAAGC CTCACAAGTG CCAGGTGTGT GGAAAAGCCT TCAGCCAAAG CTCCAACCTC 
ATCACCCACA GCCGCAAGCA CACAGGCTTC AAGCCCTTCA GCTGTGAGCT GTGCACAAAG 
GGCTTCCAGC GCAAGGTGGA CCTGCGACGT CACCGTGAGA GCCAACATAA CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1b ( XM_008761649.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759871.1 Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa47074
Clone ID Related Accession (Same CDS sequence)	XM_008761647.2 , XM_008761646.1 , XM_008761650.2 , XM_008761649.1
Accession Version	XM_008761649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGGTG TCCCAGCCAG GTCCAGTCCC 
TCGCCCAGCT TGGCTGTCCC GGGCCTCGAG GCCCCACCTG CACCTGACCC CCCAAGGCCT 
CGTTTCCTCC GGCAGGAGCG TAGCTTCGAG TGCCGGATGT GTGGCAAAGC CTTCAAGCGC 
TCATCCACTC TGTCCACCCA CCTGCTCATC CATTCGGACA CTCGGCCCTA CCCCTGCCAG 
TTCTGTGGGA AACGCTTCCA CCAGAAGTCG GACATGAAGA AACACACCTA CATCCACACA 
GGTGAGAAGC CTCACAAGTG CCAGGTGTGT GGAAAAGCCT TCAGCCAAAG CTCCAACCTC 
ATCACCCACA GCCGCAAGCA CACAGGCTTC AAGCCCTTCA GCTGTGAGCT GTGCACAAAG 
GGCTTCCAGC GCAAGGTGGA CCTGCGACGT CACCGTGAGA GCCAACATAA CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759871.1
CDS	233..1372
Translation	MPRSFLVKSKKAHTYHQPRAQGDDLVWPPAVIPGIPELMVVTLGSYLLTVAKEHTQSGSPVLSTLLPSQTLDWSAVKQKQEMLLNQSLPKMASAPEGPHVTSQPQDGESPLSESPPFYKPSFSWDTLASSYRHSYQQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFSLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDICGKTFGHAVSLEQHTHVHSQGVPARSSPSPSLAVPGLEAPPAPDPPRPRFLRQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK

Target ORF information:

RefSeq Version	XM_008761649.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761649.1

ORF Insert Sequence:

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGGTG TCCCAGCCAG GTCCAGTCCC 
TCGCCCAGCT TGGCTGTCCC GGGCCTCGAG GCCCCACCTG CACCTGACCC CCCAAGGCCT 
CGTTTCCTCC GGCAGGAGCG TAGCTTCGAG TGCCGGATGT GTGGCAAAGC CTTCAAGCGC 
TCATCCACTC TGTCCACCCA CCTGCTCATC CATTCGGACA CTCGGCCCTA CCCCTGCCAG 
TTCTGTGGGA AACGCTTCCA CCAGAAGTCG GACATGAAGA AACACACCTA CATCCACACA 
GGTGAGAAGC CTCACAAGTG CCAGGTGTGT GGAAAAGCCT TCAGCCAAAG CTCCAACCTC 
ATCACCCACA GCCGCAAGCA CACAGGCTTC AAGCCCTTCA GCTGTGAGCT GTGCACAAAG 
GGCTTCCAGC GCAAGGTGGA CCTGCGACGT CACCGTGAGA GCCAACATAA CCTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1b ( XM_008761651.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759873.1 Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa47075
Clone ID Related Accession (Same CDS sequence)	XM_008761651.1
Accession Version	XM_008761651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1b isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGAGC GTAGCTTCGA GTGCCGGATG 
TGTGGCAAAG CCTTCAAGCG CTCATCCACT CTGTCCACCC ACCTGCTCAT CCATTCGGAC 
ACTCGGCCCT ACCCCTGCCA GTTCTGTGGG AAACGCTTCC ACCAGAAGTC GGACATGAAG 
AAACACACCT ACATCCACAC AGGTGAGAAG CCTCACAAGT GCCAGGTGTG TGGAAAAGCC 
TTCAGCCAAA GCTCCAACCT CATCACCCAC AGCCGCAAGC ACACAGGCTT CAAGCCCTTC 
AGCTGTGAGC TGTGCACAAA GGGCTTCCAG CGCAAGGTGG ACCTGCGACG TCACCGTGAG 
AGCCAACATA ACCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759873.1
CDS	159..1199
Translation	MPRSFLVKSKKAHTYHQPRAQGDDLVWPPAVIPGIPELMVVTLGSYLLTVAKEHTQSGSPVLSTLLPSQTLDWSAVKQKQEMLLNQSLPKMASAPEGPHVTSQPQDGESPLSESPPFYKPSFSWDTLASSYRHSYQQTPSTMQSAFLERSVRLYGSPLVPSTESPLDFSLRYSPGMDTYHCVKCNKVFSTPHGLEVHVRRSHSGTRPFACDICGKTFGHAVSLEQHTHVHSQERSFECRMCGKAFKRSSTLSTHLLIHSDTRPYPCQFCGKRFHQKSDMKKHTYIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFSCELCTKGFQRKVDLRRHRESQHNLK

Target ORF information:

RefSeq Version	XM_008761651.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1B transcriptional repressor (Gfi1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761651.1

ORF Insert Sequence:

ATGCCGCGGT CCTTTCTAGT GAAGAGTAAG AAGGCGCACA CCTACCACCA GCCCCGAGCA 
CAGGGCGATG ACCTGGTCTG GCCTCCTGCC GTAATTCCTG GCATTCCAGA ACTGATGGTG 
GTGACTCTTG GGTCTTATCT CCTCACAGTG GCAAAAGAAC ATACCCAGAG TGGCAGCCCT 
GTTCTCAGCA CACTGCTTCC AAGCCAGACC TTGGACTGGA GCGCAGTCAA ACAGAAGCAG 
GAGATGCTGC TGAACCAGAG CCTTCCCAAG ATGGCCTCAG CCCCAGAGGG GCCTCACGTG 
ACATCCCAAC CCCAGGATGG GGAATCGCCA CTCTCCGAGT CACCCCCTTT CTATAAGCCC 
AGCTTCTCTT GGGATACGCT GGCCTCCTCC TACAGACACA GCTACCAACA GACCCCCTCC 
ACCATGCAGT CAGCTTTCCT GGAGCGCTCC GTGAGGCTGT ACGGCAGCCC GCTTGTGCCC 
AGCACCGAGT CCCCCTTGGA CTTCAGCCTC CGTTATTCTC CGGGCATGGA CACTTACCAC 
TGTGTCAAGT GTAACAAGGT GTTCTCCACC CCTCATGGGC TAGAAGTGCA TGTCCGCCGC 
TCTCACAGCG GAACCCGGCC CTTTGCCTGT GACATCTGTG GCAAAACCTT TGGCCACGCT 
GTGAGTTTGG AGCAGCACAC GCACGTGCAT TCCCAGGAGC GTAGCTTCGA GTGCCGGATG 
TGTGGCAAAG CCTTCAAGCG CTCATCCACT CTGTCCACCC ACCTGCTCAT CCATTCGGAC 
ACTCGGCCCT ACCCCTGCCA GTTCTGTGGG AAACGCTTCC ACCAGAAGTC GGACATGAAG 
AAACACACCT ACATCCACAC AGGTGAGAAG CCTCACAAGT GCCAGGTGTG TGGAAAAGCC 
TTCAGCCAAA GCTCCAACCT CATCACCCAC AGCCGCAAGC ACACAGGCTT CAAGCCCTTC 
AGCTGTGAGC TGTGCACAAA GGGCTTCCAG CGCAAGGTGG ACCTGCGACG TCACCGTGAG 
AGCCAACATA ACCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A dominant-negative GFI1B mutation in the gray platelet syndrome.
The New England journal of medicine370(3)245-53(2014 Jan)
Monteferrario D,Bolar NA,Marneth AE,Hebeda KM,Bergevoet SM,Veenstra H,Laros-van Gorkom BA,MacKenzie MA,Khandanpour C,Botezatu L,Fransen E,Van Camp G,Duijnhouwer AL,Salemink S,Willemsen B,Huls G,Preijers F,Van Heerde W,Jansen JH,Kempers MJ,Loeys BL,Van Laer L,Van der Reijden BA

Growth factor independent 1b (Gfi1b) and a new splice variant of Gfi1b are highly expressed in patients with acute and chronic leukemia.
International journal of hematology89(4)422-30(2009 May)
Vassen L,Khandanpour C,Ebeling P,van der Reijden BA,Jansen JH,Mahlmann S,D?hrsen U,M?r?y T

Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1.
Molecular cell27(4)562-72(2007 Aug)
Saleque S,Kim J,Rooke HM,Orkin SH

Growth factor-independent 1B gene (GFI1B) is overexpressed in erythropoietic and megakaryocytic malignancies and increases their proliferation rate.
British journal of haematology136(2)212-9(2007 Jan)
Elmaagacli AH,Koldehoff M,Zakrzewski JL,Steckel NK,Ottinger H,Beelen DW

GATA-1 forms distinct activating and repressive complexes in erythroid cells.
The EMBO journal24(13)2354-66(2005 Jul)
Rodriguez P,Bonte E,Krijgsveld J,Kolodziej KE,Guyot B,Heck AJ,Vyas P,de Boer E,Grosveld F,Strouboulis J

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Gfi1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

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