Wdr77 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wdr77
Entrez Gene ID	310769
Full Name	WD repeat domain 77
Synonyms	Ac2-269,MEP-50,RGD1310479
General protein information	Preferred Names WD repeat domain 77 Names methylosome protein 50 WD repeat-containing protein 77
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008771.3	NP_001008771.2	methylosome protein 50

Pathways from BioSystems

Homologies

Homo sapiens (human)	WDR77	NP_077007.1
Canis lupus familiaris (dog)	WDR77	XP_537028.1
Pan troglodytes (chimpanzee)	WDR77	NP_001233410.1
Rattus norvegicus (Norway rat)	Wdr77	NP_001008771.2
Gallus gallus (chicken)	WDR77	NP_001026087.1
Mus musculus (house mouse)	Wdr77	NP_081708.1
Xenopus tropicalis (tropical clawed frog)	wdr77	NP_001120009.1
	WDR77	XP_001105103.2
Bos taurus (cattle)	WDR77	NP_001015584.1
Rattus norvegicus (Norway rat)	LOC100911453	XP_003749421.1
Danio rerio (zebrafish)	wdr77	NP_956828.1

Gene Ontology
Bibliography

Related articles in PubMed

5-Hydroxymethylcytosine plays a critical role in glioblastomagenesis by recruiting the CHTOP-methylosome complex.
Takai H, Masuda K, Sato T, Sakaguchi Y, Suzuki T, Suzuki T, Koyama-Nasu R, Nasu-Nishimura Y, Katou Y, Ogawa H, Morishita Y, Kozuka-Hata H, Oyama M, Todo T, Ino Y, Mukasa A, Saito N, Toyoshima C, Shirahige K, Akiyama T
Cell reports9(1)48-60(2014 Oct)

RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity.
Guderian G, Peter C, Wiesner J, Sickmann A, Schulze-Osthoff K, Fischer U, Grimmler M
The Journal of biological chemistry286(3)1976-86(2011 Jan)

APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process.
Vascotto C, Fantini D, Romanello M, Cesaratto L, Deganuto M, Leonardi A, Radicella JP, Kelley MR, D'Ambrosio C, Scaloni A, Quadrifoglio F, Tell G
Molecular and cellular biology29(7)1834-54(2009 Apr)

An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs.
Chari A, Golas MM, Klingenh?ger M, Neuenkirchen N, Sander B, Englbrecht C, Sickmann A, Stark H, Fischer U
Cell135(3)497-509(2008 Oct)

Distinct nuclear and cytoplasmic functions of androgen receptor cofactor p44 and association with androgen-independent prostate cancer.
Peng Y, Chen F, Melamed J, Chiriboga L, Wei J, Kong X, McLeod M, Li Y, Li CX, Feng A, Garabedian MJ, Wang Z, Roeder RG, Lee P
Proceedings of the National Academy of Sciences of the United States of America105(13)5236-41(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Wdr77 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wdr77 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11738	NM_001008771.3 Latest version!	Rattus norvegicus WD repeat domain 77 (Wdr77), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wdr77 ( NM_001008771.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008771.2 Rattus norvegicus WD repeat domain 77 (Wdr77), mRNA.

Price & Availability↑

CloneID	ORa11738
Clone ID Related Accession (Same CDS sequence)	NM_001008771.3
Accession Version	NM_001008771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	methylosome protein 50
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB581439.1, BC097367.1 and CK842118.1. On Mar 30, 2010 this sequence version replaced NM_001008771.2. ##Evidence-Data-START## Transcript exon combination :: FQ213175.1, FQ226328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGAAGG AAAGCCCACC TCCGCTCGTG CCCCCGGCGG CCCGCGAGTG GAACCTGCCC 
CCCAATGCGC CCGCCTGCAT GGAACGGCAG TTGGAAGCTG CACGGTACCG CTCTGATGGT 
TCCCTTCTGC TCGGGGCTTC CAGTCTGAGT GGTCGCTGCT GGGCAGGATC TCTGTGGTTT 
TTCAAGGATC CTAGTGCGGC CCCCAACGAA GGTTTCTGCT CTGCTGGCGT CCAGACGGAG 
GCTGGAGTAG CTGACCTCAC TTGGGTTGGG GACAAAGGTA TCTTAGTGGC TTCTGATTCA 
GGTGCTGTTG AATTGTGGGA GCTAGATGAG AACGAGACAC TTATTGTCAG CAAGTTCTGC 
AAGTATGAGC ATGATGACAT TGTGTCTACT GTCACTGTCC TGAGCTCCGG CACACAGGCT 
GTCAGTGGTA GCAAAGACTT CTGCATCAAA ATTTGGGACC TGGCTCAGCA GATGTCGCTG 
AATTCATACC GAGCTCATGC GGGACAGGTC ACCTGTGTTG CTGCCTCTCC CCACAGAGAA 
ACTGTGTTTC TTTCATGCAG TGAGGACAGT AGAATTTTGC TCTGGGATAC CCGCTGTCCC 
AAGCCGGCAT CACAGATGGG CTGCAATGCC TCTGGCTACC TCCCTACCTC CCTGGCTTGG 
CATCCTCAGC AGAGTGAAAT CTTTGTCTTT GGTGATGAGA ATGGATCTGT CTCCCTTGTG 
GACACCAAGA ATGCAAGCTG CACCCTCAGC TCAGCTGTGC ACTCGCAGTG TGTCACTAGA 
CTGGTGTTCT CTCCACACAG TGTTCCCTTC CTGGCCTCTC TCAGTGAAGA CTGTTCACTT 
GCTGTGCTGG ATTCAAGCTT TTCTGAGGTG TTTAGAAGTC GAGCCCACAG AGACTTTGTG 
AGAGATGCTA CATGGTCTCC ACTCAATCAC TCCCTTCTTA CCACAGTTGG CTGGGACCAT 
CAGGTCATCC ACCATGTTGT GCCGTTAGAG CCTCTCCCAG CCCCTGGACC TGACAGTGTT 
GTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008771.2
CDS	97..1125
Translation	MRKESPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGSLLLGASSLSGRCWAGSLWFFKDPSAAPNEGFCSAGVQTEAGVADLTWVGDKGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVTVLSSGTQAVSGSKDFCIKIWDLAQQMSLNSYRAHAGQVTCVAASPHRETVFLSCSEDSRILLWDTRCPKPASQMGCNASGYLPTSLAWHPQQSEIFVFGDENGSVSLVDTKNASCTLSSAVHSQCVTRLVFSPHSVPFLASLSEDCSLAVLDSSFSEVFRSRAHRDFVRDATWSPLNHSLLTTVGWDHQVIHHVVPLEPLPAPGPDSVVE

Target ORF information:

RefSeq Version	NM_001008771.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WD repeat domain 77 (Wdr77), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008771.3

ORF Insert Sequence:

ATGCGGAAGG AAAGCCCACC TCCGCTCGTG CCCCCGGCGG CCCGCGAGTG GAACCTGCCC 
CCCAATGCGC CCGCCTGCAT GGAACGGCAG TTGGAAGCTG CACGGTACCG CTCTGATGGT 
TCCCTTCTGC TCGGGGCTTC CAGTCTGAGT GGTCGCTGCT GGGCAGGATC TCTGTGGTTT 
TTCAAGGATC CTAGTGCGGC CCCCAACGAA GGTTTCTGCT CTGCTGGCGT CCAGACGGAG 
GCTGGAGTAG CTGACCTCAC TTGGGTTGGG GACAAAGGTA TCTTAGTGGC TTCTGATTCA 
GGTGCTGTTG AATTGTGGGA GCTAGATGAG AACGAGACAC TTATTGTCAG CAAGTTCTGC 
AAGTATGAGC ATGATGACAT TGTGTCTACT GTCACTGTCC TGAGCTCCGG CACACAGGCT 
GTCAGTGGTA GCAAAGACTT CTGCATCAAA ATTTGGGACC TGGCTCAGCA GATGTCGCTG 
AATTCATACC GAGCTCATGC GGGACAGGTC ACCTGTGTTG CTGCCTCTCC CCACAGAGAA 
ACTGTGTTTC TTTCATGCAG TGAGGACAGT AGAATTTTGC TCTGGGATAC CCGCTGTCCC 
AAGCCGGCAT CACAGATGGG CTGCAATGCC TCTGGCTACC TCCCTACCTC CCTGGCTTGG 
CATCCTCAGC AGAGTGAAAT CTTTGTCTTT GGTGATGAGA ATGGATCTGT CTCCCTTGTG 
GACACCAAGA ATGCAAGCTG CACCCTCAGC TCAGCTGTGC ACTCGCAGTG TGTCACTAGA 
CTGGTGTTCT CTCCACACAG TGTTCCCTTC CTGGCCTCTC TCAGTGAAGA CTGTTCACTT 
GCTGTGCTGG ATTCAAGCTT TTCTGAGGTG TTTAGAAGTC GAGCCCACAG AGACTTTGTG 
AGAGATGCTA CATGGTCTCC ACTCAATCAC TCCCTTCTTA CCACAGTTGG CTGGGACCAT 
CAGGTCATCC ACCATGTTGT GCCGTTAGAG CCTCTCCCAG CCCCTGGACC TGACAGTGTT 
GTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

5-Hydroxymethylcytosine plays a critical role in glioblastomagenesis by recruiting the CHTOP-methylosome complex.
Cell reports9(1)48-60(2014 Oct)
Takai H,Masuda K,Sato T,Sakaguchi Y,Suzuki T,Suzuki T,Koyama-Nasu R,Nasu-Nishimura Y,Katou Y,Ogawa H,Morishita Y,Kozuka-Hata H,Oyama M,Todo T,Ino Y,Mukasa A,Saito N,Toyoshima C,Shirahige K,Akiyama T

RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity.
The Journal of biological chemistry286(3)1976-86(2011 Jan)
Guderian G,Peter C,Wiesner J,Sickmann A,Schulze-Osthoff K,Fischer U,Grimmler M

APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process.
Molecular and cellular biology29(7)1834-54(2009 Apr)
Vascotto C,Fantini D,Romanello M,Cesaratto L,Deganuto M,Leonardi A,Radicella JP,Kelley MR,D'Ambrosio C,Scaloni A,Quadrifoglio F,Tell G

An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs.
Cell135(3)497-509(2008 Oct)
Chari A,Golas MM,Klingenh?ger M,Neuenkirchen N,Sander B,Englbrecht C,Sickmann A,Stark H,Fischer U

Distinct nuclear and cytoplasmic functions of androgen receptor cofactor p44 and association with androgen-independent prostate cancer.
Proceedings of the National Academy of Sciences of the United States of America105(13)5236-41(2008 Apr)
Peng Y,Chen F,Melamed J,Chiriboga L,Wei J,Kong X,McLeod M,Li Y,Li CX,Feng A,Garabedian MJ,Wang Z,Roeder RG,Lee P

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Wdr77 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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