Ano1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ano1
Entrez Gene ID	309135
Full Name	anoctamin 1
Synonyms	Tmem16a
General protein information	Preferred Names anoctamin 1 Names anoctamin-1 anoctamin 1, calcium activated chloride channel transmembrane protein 16A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q42

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589235.1	XP_017444724.1	anoctamin-1 isoform X1
XM_017589236.1	XP_017444725.1	anoctamin-1 isoform X2
XM_017589239.1	XP_017444728.1	anoctamin-1 isoform X5
XM_017589237.1	XP_017444726.1	anoctamin-1 isoform X3
XM_017589238.1	XP_017444727.1	anoctamin-1 isoform X4
XM_006230780.3	XP_006230842.1	anoctamin-1 isoform X6
NM_001107564.1	NP_001101034.1	anoctamin-1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANO1	XP_854489.4
Rattus norvegicus (Norway rat)	Ano1	NP_001101034.1
Pan troglodytes (chimpanzee)	ANO1	XP_003313240.1
Mus musculus (house mouse)	Ano1	NP_848757.4
Gallus gallus (chicken)	ANO1	XP_004941586.1
Homo sapiens (human)	ANO1	NP_060513.5
Danio rerio (zebrafish)	ano1	NP_001155062.1
	LOC708723	XP_002799504.1
Bos taurus (cattle)	ANO1	NP_001179646.1
Drosophila melanogaster (fruit fly)	CG16718	NP_001262736.1
Xenopus tropicalis (tropical clawed frog)	ano1	NP_001123799.1

Gene Ontology
Bibliography

Related articles in PubMed

TMEM16A and TMEM16B channel proteins generate Ca
Yamamura H, Nishimura K, Hagihara Y, Suzuki Y, Imaizumi Y
The Journal of biological chemistry293(3)995-1006(2018 Jan)

Bile acids stimulate cholangiocyte fluid secretion by activation of membraneTMEM16A Cl
Li Q, Dutta A, Kresge C, Bugde A, Feranchak AP
Hepatology (Baltimore, Md.)(2018 Jan)

Structural basis for anion conduction in the calcium-activated chloride channel TMEM16A.
Paulino C, Neldner Y, Lam AK, Kalienkova V, Brunner JD, Schenck S, Dutzler R
eLife6(2017 May)

ANO1 inhibits cardiac fibrosis after myocardial infraction via TGF-?/smad3 pathway.
Gao Y, Zhang YM, Qian LJ, Chu M, Hong J, Xu D
Scientific reports7(1)2355(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Local coupling of TRPC6 to ANO1/TMEM16A channels in smooth muscle cells amplifies vasoconstriction in cerebral arteries.
Title: Local coupling of TRPC6 to ANO1/TMEM16A channels in smooth muscle cells amplifies vasoconstriction in cerebral arteries.

ANO1 is essential for the development of prostate hyperplasia.
Title: Anoctamin 1 (TMEM16A) is essential for testosterone-induced prostate hyperplasia.

There is functional anoctamin-1 and bestrophin-1 expression in rats at sites related to nociceptive processing.
Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats.

Overexpression of ANO1/TMEM16A, an arterial Ca2+-activated Cl- channel, contributes to spontaneous hypertension
Title: Overexpression of ANO1/TMEM16A, an arterial Ca2+-activated Cl- channel, contributes to spontaneous hypertension.

Data suggest that ANO1 is responsible for most of the iodide efflux across the apical membrane of thyroid cells.
Title: Anoctamin-1/TMEM16A is the major apical iodide channel of the thyrocyte.

The following Ano1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ano1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12697	NM_001107564.1 Latest version!	Rattus norvegicus anoctamin 1 (Ano1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$762.30 $1089.00
ORa56624	XM_017589235.1 Latest version!	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa56625	XM_017589236.1 Latest version!	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa56626	XM_017589237.1 Latest version!	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa56627	XM_017589238.1 Latest version!	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa56628	XM_017589239.1 Latest version!	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa23757	XM_006230780.3 Latest version!	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ano1 ( NM_001107564.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101034.1 Rattus norvegicus anoctamin 1 (Ano1), mRNA.

Price & Availability↑

CloneID	ORa12697
Clone ID Related Accession (Same CDS sequence)	NM_001107564.1
Accession Version	NM_001107564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	anoctamin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473953.1. On or before Oct 4, 2007 this sequence version replaced XM_219695.4, XM_001066475.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTGTA CTCAGCTGCG 
TACCCTCTGC ACGATGGTGA CTATGAAGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG CTATGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGAGAAAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCCAT TGTGGGTGTC ATTGTCTTTC TCTACGGATG TGCCACCGTG 
GACGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCGCGTGCC 
AGTCACCTCT TTGACAATCC TGCCACTGTC TTCTTTTCTG TGTTTATGGC CCTCTGGGCT 
GCCACCTTCA TGGAGCACTG GAAACGGAAG CAGATGAGAC TCAACTACCG CTGGGACCTC 
ACGGGCTTCG AGGAAGAGGA GGTGAGTAGC TGTGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACTGA CAAGGTGAAG 
TTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTTCTTGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCATCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACTGCTGTCA TCATTAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCG AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCCTATA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCT GGTGACTACG TTTATATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC AGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATCATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATCGGCAT CCCGAAGATG 
AAAAAATTCA TCCGCTACCT GAAGCTGCGC AGGCAGAGCC CCTCGGACCG CGAGGAGTAC 
ATAAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCCTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTCGTCA CCCTGTTCGT TGCGTCCTTC 
CCTCTGGCCC CACTCTTCGC CCTGCTGAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGACCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATTC TCAGAGGTGT CGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTA TACCTCTACA TGTACAGTCA AAACGGGACC 
ATGCACGGCT TCGTCAACCA CACGCTCTCC TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TACGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAGC CCCCGTGGTC AGAACACAAG TATGATATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GGCTGGCATT CGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAGGAG 
AAGGTTCTCA TGGTGGAGTT GTTCATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG TCCCACAGCC 
CACCCAGAGG CAGGTGACAG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGGCGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101034.1
CDS	51..3173
Translation	MQDTQDSDISLEGLTREGAPADRECQRGPETIVHEAQDAGTPNSGDATGAVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDVGSPEAPMDYHEDDKRFRREEYEGNLLEAGLELERDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEVSSCDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYIKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTAHPEAGDSSPVPSYEYHGGAL

Target ORF information:

RefSeq Version	NM_001107564.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus anoctamin 1 (Ano1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107564.1

ORF Insert Sequence:

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTGTA CTCAGCTGCG 
TACCCTCTGC ACGATGGTGA CTATGAAGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG CTATGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGAGAAAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCCAT TGTGGGTGTC ATTGTCTTTC TCTACGGATG TGCCACCGTG 
GACGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCGCGTGCC 
AGTCACCTCT TTGACAATCC TGCCACTGTC TTCTTTTCTG TGTTTATGGC CCTCTGGGCT 
GCCACCTTCA TGGAGCACTG GAAACGGAAG CAGATGAGAC TCAACTACCG CTGGGACCTC 
ACGGGCTTCG AGGAAGAGGA GGTGAGTAGC TGTGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACTGA CAAGGTGAAG 
TTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTTCTTGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCATCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACTGCTGTCA TCATTAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCG AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCCTATA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCT GGTGACTACG TTTATATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC AGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATCATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATCGGCAT CCCGAAGATG 
AAAAAATTCA TCCGCTACCT GAAGCTGCGC AGGCAGAGCC CCTCGGACCG CGAGGAGTAC 
ATAAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCCTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTCGTCA CCCTGTTCGT TGCGTCCTTC 
CCTCTGGCCC CACTCTTCGC CCTGCTGAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGACCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATTC TCAGAGGTGT CGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTA TACCTCTACA TGTACAGTCA AAACGGGACC 
ATGCACGGCT TCGTCAACCA CACGCTCTCC TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TACGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAGC CCCCGTGGTC AGAACACAAG TATGATATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GGCTGGCATT CGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAGGAG 
AAGGTTCTCA TGGTGGAGTT GTTCATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG TCCCACAGCC 
CACCCAGAGG CAGGTGACAG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGGCGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_017589235.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444724.1 Rattus norvegicus anoctamin 1 (Ano1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56624
Clone ID Related Accession (Same CDS sequence)	XM_017589235.1
Accession Version	XM_017589235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	anoctamin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTGTA CTCAGCTGCG 
TACCCTCTGC ACGATGGTGA CTATGAAGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG CTATGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGAGAAAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCCAT TGTGGGTGTC ATTGTCTTTC TCTACGGATG TGCCACCGTG 
GACGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCGCGTGCC 
AGTCACCTCT TTGACAATCC TGCCACTGTC TTCTTTTCTG TGTTTATGGC CCTCTGGGCT 
GCCACCTTCA TGGAGCACTG GAAACGGAAG CAGATGAGAC TCAACTACCG CTGGGACCTC 
ACGGGCTTCG AGGAAGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGCG CCGGAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACTGACA AGGTGAAGTT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TTCTTGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CATCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC TGCTGTCATC ATTAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCGAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCCTATACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCTGG TGACTACGTT 
TATATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCAG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT CATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATCGGCATCC CGAAGATGAA AAAATTCATC CGCTACCTGA AGCTGCGCAG GCAGAGCCCC 
TCGGACCGCG AGGAGTACAT AAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCCTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTCGTCACC 
CTGTTCGTTG CGTCCTTCCC TCTGGCCCCA CTCTTCGCCC TGCTGAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GACCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATTCTC AGAGGTGTCG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTATA CCTCTACATG 
TACAGTCAAA ACGGGACCAT GCACGGCTTC GTCAACCACA CGCTCTCCTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA CGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAGCCC CCGTGGTCAG AACACAAGTA TGATATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGG CTGGCATTCG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAGGAGAA GGTTCTCATG GTGGAGTTGT TCATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGTC CCACAGCCCA CCCAGAGGCA GGTGACAGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG GCGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444724.1
CDS	118..3318
Translation	MQDTQDSDISLEGLTREGAPADRECQRGPETIVHEAQDAGTPNSGDATGAVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDVGSPEAPMDYHEDDKRFRREEYEGNLLEAGLELERDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKRRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYIKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTAHPEAGDSSPVPSYEYHGGAL

Target ORF information:

RefSeq Version	XM_017589235.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589235.1

ORF Insert Sequence:

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTGTA CTCAGCTGCG 
TACCCTCTGC ACGATGGTGA CTATGAAGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG CTATGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGAGAAAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCCAT TGTGGGTGTC ATTGTCTTTC TCTACGGATG TGCCACCGTG 
GACGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCGCGTGCC 
AGTCACCTCT TTGACAATCC TGCCACTGTC TTCTTTTCTG TGTTTATGGC CCTCTGGGCT 
GCCACCTTCA TGGAGCACTG GAAACGGAAG CAGATGAGAC TCAACTACCG CTGGGACCTC 
ACGGGCTTCG AGGAAGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGAAGCG CCGGAATGTT 
CCAGAAGAAC CGACCAACAA ATGGAAGCAG AGGGTTAGGA CAGCCATGGC AGGGGTGAAA 
TTGACTGACA AGGTGAAGTT GACCTGGAGG GACCGATTCC CAGCCTATTT CACCAATCTT 
GTCTCCATCA TCTTCATGAT CGCAGTGACA TTTGCAATCG TTCTTGGAGT TATCATCTAT 
AGAATCTCCA CAGCTGCAGC CTTGGCCATG AACTCCTCCC CATCTGTGCG GTCCAACATC 
CGGGTTACAG TCACGGCCAC TGCTGTCATC ATTAACCTCG TGGTCATCAT TCTGCTGGAT 
GAAGTTTACG GCTGCATTGC CAGGTGGCTC ACCAAGATTG AGGTCCCGAA GACAGAGAAG 
AGCTTTGAGG AGAGGCTAAC CTTCAAGGCC TTCCTGCTCA AGTTTGTGAA CTCCTATACT 
CCCATCTTCT ATGTCGCCTT CTTCAAAGGC CGGTTTGTTG GTCGGCCTGG TGACTACGTT 
TATATCTTCC GCTCTTTCCG GATGGAGGAG TGTGCCCCAG GCGGCTGCCT CATGGAGCTC 
TGTATCCAGC TGAGCATCAT CATGCTGGGC AAGCAGCTAA TCCAGAACAA TCTCTTCGAG 
ATCGGCATCC CGAAGATGAA AAAATTCATC CGCTACCTGA AGCTGCGCAG GCAGAGCCCC 
TCGGACCGCG AGGAGTACAT AAAGCGGAAG CAGCGCTATG AGGTGGACTT CAACCTCGAA 
CCCTTCGCCG GCCTCACGCC CGAGTACATG GAAATGATCA TTCAGTTCGG CTTCGTCACC 
CTGTTCGTTG CGTCCTTCCC TCTGGCCCCA CTCTTCGCCC TGCTGAACAA CATCATTGAG 
ATCCGCCTGG ATGCCAAAAA GTTTGTCACC GAGCTACGGA GACCAGTAGC CATCAGAGCC 
AAAGACATCG GCATCTGGTA TAACATTCTC AGAGGTGTCG GGAAGCTGGC TGTCATCATT 
AATGCCTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTATA CCTCTACATG 
TACAGTCAAA ACGGGACCAT GCACGGCTTC GTCAACCACA CGCTCTCCTC CTTCAATGTC 
AGCGACTTCC AGAATGGCAC AGCACCCAAT GACCCACTGG ACCTGGGCTA CGAGGTTCAG 
ATCTGCAGGT ATAAAGATTA CCGGGAGCCC CCGTGGTCAG AACACAAGTA TGATATCTCC 
AAAGACTTCT GGGCTGTCCT GGCCGCCCGG CTGGCATTCG TCATTGTCTT CCAGAACCTG 
GTGATGTTCA TGAGTGACTT TGTGGACTGG GTGATCCCTG ATATCCCCAA AGACATCAGC 
CAGCAGATCC ACAAGGAGAA GGTTCTCATG GTGGAGTTGT TCATGCGTGA GGAGCAGGGC 
AAGCAGCAGC TACTGGACAC ATGGATGGAG AAGGAGAAGC CAAGGGATGT GCCTTGTAAC 
AACCACAGTC CCACAGCCCA CCCAGAGGCA GGTGACAGCA GCCCAGTCCC CAGCTACGAG 
TACCATGGGG GCGCGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_017589236.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444725.1 Rattus norvegicus anoctamin 1 (Ano1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa56625
Clone ID Related Accession (Same CDS sequence)	XM_017589236.1
Accession Version	XM_017589236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	anoctamin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGCTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCAGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTCAACTC TCTGTCCGTG 
GACCCTGACG CCGAATGCAA GTATGGCCTA TACTTCAGGG ATGGCAAACG CAAGGTGGAC 
TACATCTTGG TGTACCATCA CAAGAGAGCC TCGGGTAGCA GGACTCTGGC CAGGAGAGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGTGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGTC CTGTGGATGT GGGCTCACCG GAAGCCCCCA TGGATTACCA CGAAGATGAC 
AAGCGCTTCA GAAGGGAGGA GTACGAGGGC AACCTGTTGG AGGCAGGCCT GGAGCTGGAA 
CGTGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGCA GGGAAGCTGA GTTTTTGAAA CTGAAGATGC CCACGAAGAA GGTGTACCAC 
ATCAGTGAGA CCCGAGGCCT CCTGAAAACC ATCAACTCCG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGA CCACAGACCA CAAAGAGGCT CTCCTACCCC 
TTCTCCAGGG AGAAGCAACA CTTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GGCAAGGAGA GGGAAGAAGG AAGGACTCTG CCCTTCTAAG TAAACGGCGG 
AAGTGTGGGA AATACGGTAT CACCAGCCTC CTGGCCAATG GCGTGTACTC AGCTGCGTAC 
CCTCTGCACG ATGGTGACTA TGAAGGTGAC AACGTTGAGT TCAACGACAG GAAACTCCTG 
TATGAGGAAT GGGCAAGCTA TGGAGTCTTC TACAAATACC AGCCCATTGA CCTGGTCAGG 
AAATACTTTG GAGAAAAGGT TGGCCTGTAC TTTGCCTGGC TTGGAGCCTA CACCCAGATG 
CTCATCCCTG CCTCCATTGT GGGTGTCATT GTCTTTCTCT ACGGATGTGC CACCGTGGAC 
GAAAACATCC CCAGCATGGA GATGTGTGAC CAGAGACACA ACATCACCAT GTGTCCTCTG 
TGTGACAAGA CCTGCAGCTA CTGGAAGATG AGCTCAGCCT GTGCCACAGC GCGTGCCAGT 
CACCTCTTTG ACAATCCTGC CACTGTCTTC TTTTCTGTGT TTATGGCCCT CTGGGCTGCC 
ACCTTCATGG AGCACTGGAA ACGGAAGCAG ATGAGACTCA ACTACCGCTG GGACCTCACG 
GGCTTCGAGG AAGAGGAGGA AGCTGTCAAG GATCATCCCA GAGCAGAGTA TGAAGCCAGA 
GTCTTAGAGA AGTCACTGAG AAAAGAATCC AGAAACAAAG AGAAGCGCCG GAATGTTCCA 
GAAGAACCGA CCAACAAATG GAAGCAGAGG GTTAGGACAG CCATGGCAGG GGTGAAATTG 
ACTGACAAGG TGAAGTTGAC CTGGAGGGAC CGATTCCCAG CCTATTTCAC CAATCTTGTC 
TCCATCATCT TCATGATCGC AGTGACATTT GCAATCGTTC TTGGAGTTAT CATCTATAGA 
ATCTCCACAG CTGCAGCCTT GGCCATGAAC TCCTCCCCAT CTGTGCGGTC CAACATCCGG 
GTTACAGTCA CGGCCACTGC TGTCATCATT AACCTCGTGG TCATCATTCT GCTGGATGAA 
GTTTACGGCT GCATTGCCAG GTGGCTCACC AAGATTGAGG TCCCGAAGAC AGAGAAGAGC 
TTTGAGGAGA GGCTAACCTT CAAGGCCTTC CTGCTCAAGT TTGTGAACTC CTATACTCCC 
ATCTTCTATG TCGCCTTCTT CAAAGGCCGG TTTGTTGGTC GGCCTGGTGA CTACGTTTAT 
ATCTTCCGCT CTTTCCGGAT GGAGGAGTGT GCCCCAGGCG GCTGCCTCAT GGAGCTCTGT 
ATCCAGCTGA GCATCATCAT GCTGGGCAAG CAGCTAATCC AGAACAATCT CTTCGAGATC 
GGCATCCCGA AGATGAAAAA ATTCATCCGC TACCTGAAGC TGCGCAGGCA GAGCCCCTCG 
GACCGCGAGG AGTACATAAA GCGGAAGCAG CGCTATGAGG TGGACTTCAA CCTCGAACCC 
TTCGCCGGCC TCACGCCCGA GTACATGGAA ATGATCATTC AGTTCGGCTT CGTCACCCTG 
TTCGTTGCGT CCTTCCCTCT GGCCCCACTC TTCGCCCTGC TGAACAACAT CATTGAGATC 
CGCCTGGATG CCAAAAAGTT TGTCACCGAG CTACGGAGAC CAGTAGCCAT CAGAGCCAAA 
GACATCGGCA TCTGGTATAA CATTCTCAGA GGTGTCGGGA AGCTGGCTGT CATCATTAAT 
GCCTTTGTGA TCTCCTTCAC GTCTGACTTC ATCCCTCGCC TGGTATACCT CTACATGTAC 
AGTCAAAACG GGACCATGCA CGGCTTCGTC AACCACACGC TCTCCTCCTT CAATGTCAGC 
GACTTCCAGA ATGGCACAGC ACCCAATGAC CCACTGGACC TGGGCTACGA GGTTCAGATC 
TGCAGGTATA AAGATTACCG GGAGCCCCCG TGGTCAGAAC ACAAGTATGA TATCTCCAAA 
GACTTCTGGG CTGTCCTGGC CGCCCGGCTG GCATTCGTCA TTGTCTTCCA GAACCTGGTG 
ATGTTCATGA GTGACTTTGT GGACTGGGTG ATCCCTGATA TCCCCAAAGA CATCAGCCAG 
CAGATCCACA AGGAGAAGGT TCTCATGGTG GAGTTGTTCA TGCGTGAGGA GCAGGGCAAG 
CAGCAGCTAC TGGACACATG GATGGAGAAG GAGAAGCCAA GGGATGTGCC TTGTAACAAC 
CACAGTCCCA CAGCCCACCC AGAGGCAGGT GACAGCAGCC CAGTCCCCAG CTACGAGTAC 
CATGGGGGCG CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444725.1
CDS	118..3315
Translation	MQDTQDSDISLEGLTREGAPADRECQRGPETIVHEAQDAGTPNSDATGAVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDVGSPEAPMDYHEDDKRFRREEYEGNLLEAGLELERDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKRRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYIKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTAHPEAGDSSPVPSYEYHGGAL

Target ORF information:

RefSeq Version	XM_017589236.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589236.1

ORF Insert Sequence:

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGCTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCAGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTCAACTC TCTGTCCGTG 
GACCCTGACG CCGAATGCAA GTATGGCCTA TACTTCAGGG ATGGCAAACG CAAGGTGGAC 
TACATCTTGG TGTACCATCA CAAGAGAGCC TCGGGTAGCA GGACTCTGGC CAGGAGAGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGTGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGTC CTGTGGATGT GGGCTCACCG GAAGCCCCCA TGGATTACCA CGAAGATGAC 
AAGCGCTTCA GAAGGGAGGA GTACGAGGGC AACCTGTTGG AGGCAGGCCT GGAGCTGGAA 
CGTGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGCA GGGAAGCTGA GTTTTTGAAA CTGAAGATGC CCACGAAGAA GGTGTACCAC 
ATCAGTGAGA CCCGAGGCCT CCTGAAAACC ATCAACTCCG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGA CCACAGACCA CAAAGAGGCT CTCCTACCCC 
TTCTCCAGGG AGAAGCAACA CTTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GGCAAGGAGA GGGAAGAAGG AAGGACTCTG CCCTTCTAAG TAAACGGCGG 
AAGTGTGGGA AATACGGTAT CACCAGCCTC CTGGCCAATG GCGTGTACTC AGCTGCGTAC 
CCTCTGCACG ATGGTGACTA TGAAGGTGAC AACGTTGAGT TCAACGACAG GAAACTCCTG 
TATGAGGAAT GGGCAAGCTA TGGAGTCTTC TACAAATACC AGCCCATTGA CCTGGTCAGG 
AAATACTTTG GAGAAAAGGT TGGCCTGTAC TTTGCCTGGC TTGGAGCCTA CACCCAGATG 
CTCATCCCTG CCTCCATTGT GGGTGTCATT GTCTTTCTCT ACGGATGTGC CACCGTGGAC 
GAAAACATCC CCAGCATGGA GATGTGTGAC CAGAGACACA ACATCACCAT GTGTCCTCTG 
TGTGACAAGA CCTGCAGCTA CTGGAAGATG AGCTCAGCCT GTGCCACAGC GCGTGCCAGT 
CACCTCTTTG ACAATCCTGC CACTGTCTTC TTTTCTGTGT TTATGGCCCT CTGGGCTGCC 
ACCTTCATGG AGCACTGGAA ACGGAAGCAG ATGAGACTCA ACTACCGCTG GGACCTCACG 
GGCTTCGAGG AAGAGGAGGA AGCTGTCAAG GATCATCCCA GAGCAGAGTA TGAAGCCAGA 
GTCTTAGAGA AGTCACTGAG AAAAGAATCC AGAAACAAAG AGAAGCGCCG GAATGTTCCA 
GAAGAACCGA CCAACAAATG GAAGCAGAGG GTTAGGACAG CCATGGCAGG GGTGAAATTG 
ACTGACAAGG TGAAGTTGAC CTGGAGGGAC CGATTCCCAG CCTATTTCAC CAATCTTGTC 
TCCATCATCT TCATGATCGC AGTGACATTT GCAATCGTTC TTGGAGTTAT CATCTATAGA 
ATCTCCACAG CTGCAGCCTT GGCCATGAAC TCCTCCCCAT CTGTGCGGTC CAACATCCGG 
GTTACAGTCA CGGCCACTGC TGTCATCATT AACCTCGTGG TCATCATTCT GCTGGATGAA 
GTTTACGGCT GCATTGCCAG GTGGCTCACC AAGATTGAGG TCCCGAAGAC AGAGAAGAGC 
TTTGAGGAGA GGCTAACCTT CAAGGCCTTC CTGCTCAAGT TTGTGAACTC CTATACTCCC 
ATCTTCTATG TCGCCTTCTT CAAAGGCCGG TTTGTTGGTC GGCCTGGTGA CTACGTTTAT 
ATCTTCCGCT CTTTCCGGAT GGAGGAGTGT GCCCCAGGCG GCTGCCTCAT GGAGCTCTGT 
ATCCAGCTGA GCATCATCAT GCTGGGCAAG CAGCTAATCC AGAACAATCT CTTCGAGATC 
GGCATCCCGA AGATGAAAAA ATTCATCCGC TACCTGAAGC TGCGCAGGCA GAGCCCCTCG 
GACCGCGAGG AGTACATAAA GCGGAAGCAG CGCTATGAGG TGGACTTCAA CCTCGAACCC 
TTCGCCGGCC TCACGCCCGA GTACATGGAA ATGATCATTC AGTTCGGCTT CGTCACCCTG 
TTCGTTGCGT CCTTCCCTCT GGCCCCACTC TTCGCCCTGC TGAACAACAT CATTGAGATC 
CGCCTGGATG CCAAAAAGTT TGTCACCGAG CTACGGAGAC CAGTAGCCAT CAGAGCCAAA 
GACATCGGCA TCTGGTATAA CATTCTCAGA GGTGTCGGGA AGCTGGCTGT CATCATTAAT 
GCCTTTGTGA TCTCCTTCAC GTCTGACTTC ATCCCTCGCC TGGTATACCT CTACATGTAC 
AGTCAAAACG GGACCATGCA CGGCTTCGTC AACCACACGC TCTCCTCCTT CAATGTCAGC 
GACTTCCAGA ATGGCACAGC ACCCAATGAC CCACTGGACC TGGGCTACGA GGTTCAGATC 
TGCAGGTATA AAGATTACCG GGAGCCCCCG TGGTCAGAAC ACAAGTATGA TATCTCCAAA 
GACTTCTGGG CTGTCCTGGC CGCCCGGCTG GCATTCGTCA TTGTCTTCCA GAACCTGGTG 
ATGTTCATGA GTGACTTTGT GGACTGGGTG ATCCCTGATA TCCCCAAAGA CATCAGCCAG 
CAGATCCACA AGGAGAAGGT TCTCATGGTG GAGTTGTTCA TGCGTGAGGA GCAGGGCAAG 
CAGCAGCTAC TGGACACATG GATGGAGAAG GAGAAGCCAA GGGATGTGCC TTGTAACAAC 
CACAGTCCCA CAGCCCACCC AGAGGCAGGT GACAGCAGCC CAGTCCCCAG CTACGAGTAC 
CATGGGGGCG CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_017589237.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444726.1 Rattus norvegicus anoctamin 1 (Ano1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa56626
Clone ID Related Accession (Same CDS sequence)	XM_017589237.1
Accession Version	XM_017589237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	anoctamin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGTATCAC CAGCCTCCTG GCCAATGGCG TGTACTCAGC TGCGTACCCT 
CTGCACGATG GTGACTATGA AGGTGACAAC GTTGAGTTCA ACGACAGGAA ACTCCTGTAT 
GAGGAATGGG CAAGCTATGG AGTCTTCTAC AAATACCAGC CCATTGACCT GGTCAGGAAA 
TACTTTGGAG AAAAGGTTGG CCTGTACTTT GCCTGGCTTG GAGCCTACAC CCAGATGCTC 
ATCCCTGCCT CCATTGTGGG TGTCATTGTC TTTCTCTACG GATGTGCCAC CGTGGACGAA 
AACATCCCCA GCATGGAGAT GTGTGACCAG AGACACAACA TCACCATGTG TCCTCTGTGT 
GACAAGACCT GCAGCTACTG GAAGATGAGC TCAGCCTGTG CCACAGCGCG TGCCAGTCAC 
CTCTTTGACA ATCCTGCCAC TGTCTTCTTT TCTGTGTTTA TGGCCCTCTG GGCTGCCACC 
TTCATGGAGC ACTGGAAACG GAAGCAGATG AGACTCAACT ACCGCTGGGA CCTCACGGGC 
TTCGAGGAAG AGGAGGAAGC TGTCAAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA AGCGCCGGAA TGTTCCAGAA 
GAACCGACCA ACAAATGGAA GCAGAGGGTT AGGACAGCCA TGGCAGGGGT GAAATTGACT 
GACAAGGTGA AGTTGACCTG GAGGGACCGA TTCCCAGCCT ATTTCACCAA TCTTGTCTCC 
ATCATCTTCA TGATCGCAGT GACATTTGCA ATCGTTCTTG GAGTTATCAT CTATAGAATC 
TCCACAGCTG CAGCCTTGGC CATGAACTCC TCCCCATCTG TGCGGTCCAA CATCCGGGTT 
ACAGTCACGG CCACTGCTGT CATCATTAAC CTCGTGGTCA TCATTCTGCT GGATGAAGTT 
TACGGCTGCA TTGCCAGGTG GCTCACCAAG ATTGAGGTCC CGAAGACAGA GAAGAGCTTT 
GAGGAGAGGC TAACCTTCAA GGCCTTCCTG CTCAAGTTTG TGAACTCCTA TACTCCCATC 
TTCTATGTCG CCTTCTTCAA AGGCCGGTTT GTTGGTCGGC CTGGTGACTA CGTTTATATC 
TTCCGCTCTT TCCGGATGGA GGAGTGTGCC CCAGGCGGCT GCCTCATGGA GCTCTGTATC 
CAGCTGAGCA TCATCATGCT GGGCAAGCAG CTAATCCAGA ACAATCTCTT CGAGATCGGC 
ATCCCGAAGA TGAAAAAATT CATCCGCTAC CTGAAGCTGC GCAGGCAGAG CCCCTCGGAC 
CGCGAGGAGT ACATAAAGCG GAAGCAGCGC TATGAGGTGG ACTTCAACCT CGAACCCTTC 
GCCGGCCTCA CGCCCGAGTA CATGGAAATG ATCATTCAGT TCGGCTTCGT CACCCTGTTC 
GTTGCGTCCT TCCCTCTGGC CCCACTCTTC GCCCTGCTGA ACAACATCAT TGAGATCCGC 
CTGGATGCCA AAAAGTTTGT CACCGAGCTA CGGAGACCAG TAGCCATCAG AGCCAAAGAC 
ATCGGCATCT GGTATAACAT TCTCAGAGGT GTCGGGAAGC TGGCTGTCAT CATTAATGCC 
TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TATACCTCTA CATGTACAGT 
CAAAACGGGA CCATGCACGG CTTCGTCAAC CACACGCTCT CCTCCTTCAA TGTCAGCGAC 
TTCCAGAATG GCACAGCACC CAATGACCCA CTGGACCTGG GCTACGAGGT TCAGATCTGC 
AGGTATAAAG ATTACCGGGA GCCCCCGTGG TCAGAACACA AGTATGATAT CTCCAAAGAC 
TTCTGGGCTG TCCTGGCCGC CCGGCTGGCA TTCGTCATTG TCTTCCAGAA CCTGGTGATG 
TTCATGAGTG ACTTTGTGGA CTGGGTGATC CCTGATATCC CCAAAGACAT CAGCCAGCAG 
ATCCACAAGG AGAAGGTTCT CATGGTGGAG TTGTTCATGC GTGAGGAGCA GGGCAAGCAG 
CAGCTACTGG ACACATGGAT GGAGAAGGAG AAGCCAAGGG ATGTGCCTTG TAACAACCAC 
AGTCCCACAG CCCACCCAGA GGCAGGTGAC AGCAGCCCAG TCCCCAGCTA CGAGTACCAT 
GGGGGCGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444726.1
CDS	118..3252
Translation	MQDTQDSDISLEGLTREGAPADRECQRGPETIVHEAQDAGTPNSGDATGAVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDVGSPEAPMDYHEDDKRFRREEYEGNLLEAGLELERDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKEKRRNVPEEPTNKWKQRVRTAMAGVKLTDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYIKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTAHPEAGDSSPVPSYEYHGGAL

Target ORF information:

RefSeq Version	XM_017589237.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589237.1

ORF Insert Sequence:

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGTATCAC CAGCCTCCTG GCCAATGGCG TGTACTCAGC TGCGTACCCT 
CTGCACGATG GTGACTATGA AGGTGACAAC GTTGAGTTCA ACGACAGGAA ACTCCTGTAT 
GAGGAATGGG CAAGCTATGG AGTCTTCTAC AAATACCAGC CCATTGACCT GGTCAGGAAA 
TACTTTGGAG AAAAGGTTGG CCTGTACTTT GCCTGGCTTG GAGCCTACAC CCAGATGCTC 
ATCCCTGCCT CCATTGTGGG TGTCATTGTC TTTCTCTACG GATGTGCCAC CGTGGACGAA 
AACATCCCCA GCATGGAGAT GTGTGACCAG AGACACAACA TCACCATGTG TCCTCTGTGT 
GACAAGACCT GCAGCTACTG GAAGATGAGC TCAGCCTGTG CCACAGCGCG TGCCAGTCAC 
CTCTTTGACA ATCCTGCCAC TGTCTTCTTT TCTGTGTTTA TGGCCCTCTG GGCTGCCACC 
TTCATGGAGC ACTGGAAACG GAAGCAGATG AGACTCAACT ACCGCTGGGA CCTCACGGGC 
TTCGAGGAAG AGGAGGAAGC TGTCAAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA AGCGCCGGAA TGTTCCAGAA 
GAACCGACCA ACAAATGGAA GCAGAGGGTT AGGACAGCCA TGGCAGGGGT GAAATTGACT 
GACAAGGTGA AGTTGACCTG GAGGGACCGA TTCCCAGCCT ATTTCACCAA TCTTGTCTCC 
ATCATCTTCA TGATCGCAGT GACATTTGCA ATCGTTCTTG GAGTTATCAT CTATAGAATC 
TCCACAGCTG CAGCCTTGGC CATGAACTCC TCCCCATCTG TGCGGTCCAA CATCCGGGTT 
ACAGTCACGG CCACTGCTGT CATCATTAAC CTCGTGGTCA TCATTCTGCT GGATGAAGTT 
TACGGCTGCA TTGCCAGGTG GCTCACCAAG ATTGAGGTCC CGAAGACAGA GAAGAGCTTT 
GAGGAGAGGC TAACCTTCAA GGCCTTCCTG CTCAAGTTTG TGAACTCCTA TACTCCCATC 
TTCTATGTCG CCTTCTTCAA AGGCCGGTTT GTTGGTCGGC CTGGTGACTA CGTTTATATC 
TTCCGCTCTT TCCGGATGGA GGAGTGTGCC CCAGGCGGCT GCCTCATGGA GCTCTGTATC 
CAGCTGAGCA TCATCATGCT GGGCAAGCAG CTAATCCAGA ACAATCTCTT CGAGATCGGC 
ATCCCGAAGA TGAAAAAATT CATCCGCTAC CTGAAGCTGC GCAGGCAGAG CCCCTCGGAC 
CGCGAGGAGT ACATAAAGCG GAAGCAGCGC TATGAGGTGG ACTTCAACCT CGAACCCTTC 
GCCGGCCTCA CGCCCGAGTA CATGGAAATG ATCATTCAGT TCGGCTTCGT CACCCTGTTC 
GTTGCGTCCT TCCCTCTGGC CCCACTCTTC GCCCTGCTGA ACAACATCAT TGAGATCCGC 
CTGGATGCCA AAAAGTTTGT CACCGAGCTA CGGAGACCAG TAGCCATCAG AGCCAAAGAC 
ATCGGCATCT GGTATAACAT TCTCAGAGGT GTCGGGAAGC TGGCTGTCAT CATTAATGCC 
TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TATACCTCTA CATGTACAGT 
CAAAACGGGA CCATGCACGG CTTCGTCAAC CACACGCTCT CCTCCTTCAA TGTCAGCGAC 
TTCCAGAATG GCACAGCACC CAATGACCCA CTGGACCTGG GCTACGAGGT TCAGATCTGC 
AGGTATAAAG ATTACCGGGA GCCCCCGTGG TCAGAACACA AGTATGATAT CTCCAAAGAC 
TTCTGGGCTG TCCTGGCCGC CCGGCTGGCA TTCGTCATTG TCTTCCAGAA CCTGGTGATG 
TTCATGAGTG ACTTTGTGGA CTGGGTGATC CCTGATATCC CCAAAGACAT CAGCCAGCAG 
ATCCACAAGG AGAAGGTTCT CATGGTGGAG TTGTTCATGC GTGAGGAGCA GGGCAAGCAG 
CAGCTACTGG ACACATGGAT GGAGAAGGAG AAGCCAAGGG ATGTGCCTTG TAACAACCAC 
AGTCCCACAG CCCACCCAGA GGCAGGTGAC AGCAGCCCAG TCCCCAGCTA CGAGTACCAT 
GGGGGCGCGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_017589238.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444727.1 Rattus norvegicus anoctamin 1 (Ano1), transcript variant X4, mRNA.

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CloneID	ORa56627
Clone ID Related Accession (Same CDS sequence)	XM_017589238.1
Accession Version	XM_017589238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	anoctamin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTGTA CTCAGCTGCG 
TACCCTCTGC ACGATGGTGA CTATGAAGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG CTATGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGAGAAAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCCAT TGTGGGTGTC ATTGTCTTTC TCTACGGATG TGCCACCGTG 
GACGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCGCGTGCC 
AGTCACCTCT TTGACAATCC TGCCACTGTC TTCTTTTCTG TGTTTATGGC CCTCTGGGCT 
GCCACCTTCA TGGAGCACTG GAAACGGAAG CAGATGAGAC TCAACTACCG CTGGGACCTC 
ACGGGCTTCG AGGAAGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACTGA CAAGGTGAAG 
TTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTTCTTGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCATCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACTGCTGTCA TCATTAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCG AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCCTATA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCT GGTGACTACG TTTATATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC AGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATCATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATCGGCAT CCCGAAGATG 
AAAAAATTCA TCCGCTACCT GAAGCTGCGC AGGCAGAGCC CCTCGGACCG CGAGGAGTAC 
ATAAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCCTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTCGTCA CCCTGTTCGT TGCGTCCTTC 
CCTCTGGCCC CACTCTTCGC CCTGCTGAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGACCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATTC TCAGAGGTGT CGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTA TACCTCTACA TGTACAGTCA AAACGGGACC 
ATGCACGGCT TCGTCAACCA CACGCTCTCC TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TACGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAGC CCCCGTGGTC AGAACACAAG TATGATATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GGCTGGCATT CGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAGGAG 
AAGGTTCTCA TGGTGGAGTT GTTCATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG TCCCACAGCC 
CACCCAGAGG CAGGTGACAG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGGCGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444727.1
CDS	117..3239
Translation	MQDTQDSDISLEGLTREGAPADRECQRGPETIVHEAQDAGTPNSGDATGAVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDVGSPEAPMDYHEDDKRFRREEYEGNLLEAGLELERDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGQGEGRRKDSALLSKRRKCGKYGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYIKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTAHPEAGDSSPVPSYEYHGGAL

Target ORF information:

RefSeq Version	XM_017589238.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589238.1

ORF Insert Sequence:

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGGCAAGG AGAGGGAAGA AGGAAGGACT CTGCCCTTCT AAGTAAACGG 
CGGAAGTGTG GGAAATACGG TATCACCAGC CTCCTGGCCA ATGGCGTGTA CTCAGCTGCG 
TACCCTCTGC ACGATGGTGA CTATGAAGGT GACAACGTTG AGTTCAACGA CAGGAAACTC 
CTGTATGAGG AATGGGCAAG CTATGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC 
AGGAAATACT TTGGAGAAAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG 
ATGCTCATCC CTGCCTCCAT TGTGGGTGTC ATTGTCTTTC TCTACGGATG TGCCACCGTG 
GACGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAACATCAC CATGTGTCCT 
CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCGCGTGCC 
AGTCACCTCT TTGACAATCC TGCCACTGTC TTCTTTTCTG TGTTTATGGC CCTCTGGGCT 
GCCACCTTCA TGGAGCACTG GAAACGGAAG CAGATGAGAC TCAACTACCG CTGGGACCTC 
ACGGGCTTCG AGGAAGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC 
AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACTGA CAAGGTGAAG 
TTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG 
ATCGCAGTGA CATTTGCAAT CGTTCTTGGA GTTATCATCT ATAGAATCTC CACAGCTGCA 
GCCTTGGCCA TGAACTCCTC CCCATCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC 
ACTGCTGTCA TCATTAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT 
GCCAGGTGGC TCACCAAGAT TGAGGTCCCG AAGACAGAGA AGAGCTTTGA GGAGAGGCTA 
ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCCTATA CTCCCATCTT CTATGTCGCC 
TTCTTCAAAG GCCGGTTTGT TGGTCGGCCT GGTGACTACG TTTATATCTT CCGCTCTTTC 
CGGATGGAGG AGTGTGCCCC AGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC 
ATCATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATCGGCAT CCCGAAGATG 
AAAAAATTCA TCCGCTACCT GAAGCTGCGC AGGCAGAGCC CCTCGGACCG CGAGGAGTAC 
ATAAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCCTTCGC CGGCCTCACG 
CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTCGTCA CCCTGTTCGT TGCGTCCTTC 
CCTCTGGCCC CACTCTTCGC CCTGCTGAAC AACATCATTG AGATCCGCCT GGATGCCAAA 
AAGTTTGTCA CCGAGCTACG GAGACCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG 
TATAACATTC TCAGAGGTGT CGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC 
TTCACGTCTG ACTTCATCCC TCGCCTGGTA TACCTCTACA TGTACAGTCA AAACGGGACC 
ATGCACGGCT TCGTCAACCA CACGCTCTCC TCCTTCAATG TCAGCGACTT CCAGAATGGC 
ACAGCACCCA ATGACCCACT GGACCTGGGC TACGAGGTTC AGATCTGCAG GTATAAAGAT 
TACCGGGAGC CCCCGTGGTC AGAACACAAG TATGATATCT CCAAAGACTT CTGGGCTGTC 
CTGGCCGCCC GGCTGGCATT CGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC 
TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAGGAG 
AAGGTTCTCA TGGTGGAGTT GTTCATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC 
ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG TCCCACAGCC 
CACCCAGAGG CAGGTGACAG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGGCGCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_017589239.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444728.1 Rattus norvegicus anoctamin 1 (Ano1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa56628
Clone ID Related Accession (Same CDS sequence)	XM_017589239.1
Accession Version	XM_017589239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	anoctamin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGTATCAC CAGCCTCCTG GCCAATGGCG TGTACTCAGC TGCGTACCCT 
CTGCACGATG GTGACTATGA AGGTGACAAC GTTGAGTTCA ACGACAGGAA ACTCCTGTAT 
GAGGAATGGG CAAGCTATGG AGTCTTCTAC AAATACCAGC CCATTGACCT GGTCAGGAAA 
TACTTTGGAG AAAAGGTTGG CCTGTACTTT GCCTGGCTTG GAGCCTACAC CCAGATGCTC 
ATCCCTGCCT CCATTGTGGG TGTCATTGTC TTTCTCTACG GATGTGCCAC CGTGGACGAA 
AACATCCCCA GCATGGAGAT GTGTGACCAG AGACACAACA TCACCATGTG TCCTCTGTGT 
GACAAGACCT GCAGCTACTG GAAGATGAGC TCAGCCTGTG CCACAGCGCG TGCCAGTCAC 
CTCTTTGACA ATCCTGCCAC TGTCTTCTTT TCTGTGTTTA TGGCCCTCTG GGCTGCCACC 
TTCATGGAGC ACTGGAAACG GAAGCAGATG AGACTCAACT ACCGCTGGGA CCTCACGGGC 
TTCGAGGAAG AGGAGGAAGC TGTCAAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA CTGACAAGGT GAAGTTGACC 
TGGAGGGACC GATTCCCAGC CTATTTCACC AATCTTGTCT CCATCATCTT CATGATCGCA 
GTGACATTTG CAATCGTTCT TGGAGTTATC ATCTATAGAA TCTCCACAGC TGCAGCCTTG 
GCCATGAACT CCTCCCCATC TGTGCGGTCC AACATCCGGG TTACAGTCAC GGCCACTGCT 
GTCATCATTA ACCTCGTGGT CATCATTCTG CTGGATGAAG TTTACGGCTG CATTGCCAGG 
TGGCTCACCA AGATTGAGGT CCCGAAGACA GAGAAGAGCT TTGAGGAGAG GCTAACCTTC 
AAGGCCTTCC TGCTCAAGTT TGTGAACTCC TATACTCCCA TCTTCTATGT CGCCTTCTTC 
AAAGGCCGGT TTGTTGGTCG GCCTGGTGAC TACGTTTATA TCTTCCGCTC TTTCCGGATG 
GAGGAGTGTG CCCCAGGCGG CTGCCTCATG GAGCTCTGTA TCCAGCTGAG CATCATCATG 
CTGGGCAAGC AGCTAATCCA GAACAATCTC TTCGAGATCG GCATCCCGAA GATGAAAAAA 
TTCATCCGCT ACCTGAAGCT GCGCAGGCAG AGCCCCTCGG ACCGCGAGGA GTACATAAAG 
CGGAAGCAGC GCTATGAGGT GGACTTCAAC CTCGAACCCT TCGCCGGCCT CACGCCCGAG 
TACATGGAAA TGATCATTCA GTTCGGCTTC GTCACCCTGT TCGTTGCGTC CTTCCCTCTG 
GCCCCACTCT TCGCCCTGCT GAACAACATC ATTGAGATCC GCCTGGATGC CAAAAAGTTT 
GTCACCGAGC TACGGAGACC AGTAGCCATC AGAGCCAAAG ACATCGGCAT CTGGTATAAC 
ATTCTCAGAG GTGTCGGGAA GCTGGCTGTC ATCATTAATG CCTTTGTGAT CTCCTTCACG 
TCTGACTTCA TCCCTCGCCT GGTATACCTC TACATGTACA GTCAAAACGG GACCATGCAC 
GGCTTCGTCA ACCACACGCT CTCCTCCTTC AATGTCAGCG ACTTCCAGAA TGGCACAGCA 
CCCAATGACC CACTGGACCT GGGCTACGAG GTTCAGATCT GCAGGTATAA AGATTACCGG 
GAGCCCCCGT GGTCAGAACA CAAGTATGAT ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC 
GCCCGGCTGG CATTCGTCAT TGTCTTCCAG AACCTGGTGA TGTTCATGAG TGACTTTGTG 
GACTGGGTGA TCCCTGATAT CCCCAAAGAC ATCAGCCAGC AGATCCACAA GGAGAAGGTT 
CTCATGGTGG AGTTGTTCAT GCGTGAGGAG CAGGGCAAGC AGCAGCTACT GGACACATGG 
ATGGAGAAGG AGAAGCCAAG GGATGTGCCT TGTAACAACC ACAGTCCCAC AGCCCACCCA 
GAGGCAGGTG ACAGCAGCCC AGTCCCCAGC TACGAGTACC ATGGGGGCGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444728.1
CDS	117..3173
Translation	MQDTQDSDISLEGLTREGAPADRECQRGPETIVHEAQDAGTPNSGDATGAVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDVGSPEAPMDYHEDDKRFRREEYEGNLLEAGLELERDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYIKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTAHPEAGDSSPVPSYEYHGGAL

Target ORF information:

RefSeq Version	XM_017589239.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589239.1

ORF Insert Sequence:

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGGTGACGC CACCGGGGCT GTGGATGGGG AGCGCGAGGC CACCATGAGG 
GTCCCCGAGA AGTACTCGAC GCTCCCAGCG GAGGACCGCA GCGTCCACAT CGTGAACATC 
TGCGCCATCG AGGACCTGGG CTACCTGCCG TCCGAGGGCA CGTTGCTCAA CTCTCTGTCC 
GTGGACCCTG ACGCCGAATG CAAGTATGGC CTATACTTCA GGGATGGCAA ACGCAAGGTG 
GACTACATCT TGGTGTACCA TCACAAGAGA GCCTCGGGTA GCAGGACTCT GGCCAGGAGA 
GGACTACAAA ATGACATGGT CCTGGGGACC CGCAGTGTCA GGCAGGACCA GCCCCTTCCC 
GGGAAGGGGA GTCCTGTGGA TGTGGGCTCA CCGGAAGCCC CCATGGATTA CCACGAAGAT 
GACAAGCGCT TCAGAAGGGA GGAGTACGAG GGCAACCTGT TGGAGGCAGG CCTGGAGCTG 
GAACGTGACG AGGATACCAA AATCCATGGT GTCGGGTTTG TGAAGATCCA TGCGCCCTGG 
CATGTGCTCT GCAGGGAAGC TGAGTTTTTG AAACTGAAGA TGCCCACGAA GAAGGTGTAC 
CACATCAGTG AGACCCGAGG CCTCCTGAAA ACCATCAACT CCGTTCTGCA GAAGATCACA 
GACCCCATCC AGCCCAAGGT GGCTGAGCAC AGACCACAGA CCACAAAGAG GCTCTCCTAC 
CCCTTCTCCA GGGAGAAGCA ACACTTATTC GACCTGACTG ACAGGGACTC TTTTTTCGAC 
AGCAAAACCC GGAGCACAAT AGTCTATGAA ATCCTGAAGA GAACAACGTG CACCAAGGCC 
AAGTACAGCA TGGGTATCAC CAGCCTCCTG GCCAATGGCG TGTACTCAGC TGCGTACCCT 
CTGCACGATG GTGACTATGA AGGTGACAAC GTTGAGTTCA ACGACAGGAA ACTCCTGTAT 
GAGGAATGGG CAAGCTATGG AGTCTTCTAC AAATACCAGC CCATTGACCT GGTCAGGAAA 
TACTTTGGAG AAAAGGTTGG CCTGTACTTT GCCTGGCTTG GAGCCTACAC CCAGATGCTC 
ATCCCTGCCT CCATTGTGGG TGTCATTGTC TTTCTCTACG GATGTGCCAC CGTGGACGAA 
AACATCCCCA GCATGGAGAT GTGTGACCAG AGACACAACA TCACCATGTG TCCTCTGTGT 
GACAAGACCT GCAGCTACTG GAAGATGAGC TCAGCCTGTG CCACAGCGCG TGCCAGTCAC 
CTCTTTGACA ATCCTGCCAC TGTCTTCTTT TCTGTGTTTA TGGCCCTCTG GGCTGCCACC 
TTCATGGAGC ACTGGAAACG GAAGCAGATG AGACTCAACT ACCGCTGGGA CCTCACGGGC 
TTCGAGGAAG AGGAGGAAGC TGTCAAGGAT CATCCCAGAG CAGAGTATGA AGCCAGAGTC 
TTAGAGAAGT CACTGAGAAA AGAATCCAGA AACAAAGAGA CTGACAAGGT GAAGTTGACC 
TGGAGGGACC GATTCCCAGC CTATTTCACC AATCTTGTCT CCATCATCTT CATGATCGCA 
GTGACATTTG CAATCGTTCT TGGAGTTATC ATCTATAGAA TCTCCACAGC TGCAGCCTTG 
GCCATGAACT CCTCCCCATC TGTGCGGTCC AACATCCGGG TTACAGTCAC GGCCACTGCT 
GTCATCATTA ACCTCGTGGT CATCATTCTG CTGGATGAAG TTTACGGCTG CATTGCCAGG 
TGGCTCACCA AGATTGAGGT CCCGAAGACA GAGAAGAGCT TTGAGGAGAG GCTAACCTTC 
AAGGCCTTCC TGCTCAAGTT TGTGAACTCC TATACTCCCA TCTTCTATGT CGCCTTCTTC 
AAAGGCCGGT TTGTTGGTCG GCCTGGTGAC TACGTTTATA TCTTCCGCTC TTTCCGGATG 
GAGGAGTGTG CCCCAGGCGG CTGCCTCATG GAGCTCTGTA TCCAGCTGAG CATCATCATG 
CTGGGCAAGC AGCTAATCCA GAACAATCTC TTCGAGATCG GCATCCCGAA GATGAAAAAA 
TTCATCCGCT ACCTGAAGCT GCGCAGGCAG AGCCCCTCGG ACCGCGAGGA GTACATAAAG 
CGGAAGCAGC GCTATGAGGT GGACTTCAAC CTCGAACCCT TCGCCGGCCT CACGCCCGAG 
TACATGGAAA TGATCATTCA GTTCGGCTTC GTCACCCTGT TCGTTGCGTC CTTCCCTCTG 
GCCCCACTCT TCGCCCTGCT GAACAACATC ATTGAGATCC GCCTGGATGC CAAAAAGTTT 
GTCACCGAGC TACGGAGACC AGTAGCCATC AGAGCCAAAG ACATCGGCAT CTGGTATAAC 
ATTCTCAGAG GTGTCGGGAA GCTGGCTGTC ATCATTAATG CCTTTGTGAT CTCCTTCACG 
TCTGACTTCA TCCCTCGCCT GGTATACCTC TACATGTACA GTCAAAACGG GACCATGCAC 
GGCTTCGTCA ACCACACGCT CTCCTCCTTC AATGTCAGCG ACTTCCAGAA TGGCACAGCA 
CCCAATGACC CACTGGACCT GGGCTACGAG GTTCAGATCT GCAGGTATAA AGATTACCGG 
GAGCCCCCGT GGTCAGAACA CAAGTATGAT ATCTCCAAAG ACTTCTGGGC TGTCCTGGCC 
GCCCGGCTGG CATTCGTCAT TGTCTTCCAG AACCTGGTGA TGTTCATGAG TGACTTTGTG 
GACTGGGTGA TCCCTGATAT CCCCAAAGAC ATCAGCCAGC AGATCCACAA GGAGAAGGTT 
CTCATGGTGG AGTTGTTCAT GCGTGAGGAG CAGGGCAAGC AGCAGCTACT GGACACATGG 
ATGGAGAAGG AGAAGCCAAG GGATGTGCCT TGTAACAACC ACAGTCCCAC AGCCCACCCA 
GAGGCAGGTG ACAGCAGCCC AGTCCCCAGC TACGAGTACC ATGGGGGCGC GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano1 ( XM_006230780.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230842.1 Rattus norvegicus anoctamin 1 (Ano1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa23757
Clone ID Related Accession (Same CDS sequence)	XM_006230780.3
Accession Version	XM_006230780.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	anoctamin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230780.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGCTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCAGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTCAACTC TCTGTCCGTG 
GACCCTGACG CCGAATGCAA GTATGGCCTA TACTTCAGGG ATGGCAAACG CAAGGTGGAC 
TACATCTTGG TGTACCATCA CAAGAGAGCC TCGGGTAGCA GGACTCTGGC CAGGAGAGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGTGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGTC CTGTGGATGT GGGCTCACCG GAAGCCCCCA TGGATTACCA CGAAGATGAC 
AAGCGCTTCA GAAGGGAGGA GTACGAGGGC AACCTGTTGG AGGCAGGCCT GGAGCTGGAA 
CGTGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGCA GGGAAGCTGA GTTTTTGAAA CTGAAGATGC CCACGAAGAA GGTGTACCAC 
ATCAGTGAGA CCCGAGGCCT CCTGAAAACC ATCAACTCCG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGA CCACAGACCA CAAAGAGGCT CTCCTACCCC 
TTCTCCAGGG AGAAGCAACA CTTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GTATCACCAG CCTCCTGGCC AATGGCGTGT ACTCAGCTGC GTACCCTCTG 
CACGATGGTG ACTATGAAGG TGACAACGTT GAGTTCAACG ACAGGAAACT CCTGTATGAG 
GAATGGGCAA GCTATGGAGT CTTCTACAAA TACCAGCCCA TTGACCTGGT CAGGAAATAC 
TTTGGAGAAA AGGTTGGCCT GTACTTTGCC TGGCTTGGAG CCTACACCCA GATGCTCATC 
CCTGCCTCCA TTGTGGGTGT CATTGTCTTT CTCTACGGAT GTGCCACCGT GGACGAAAAC 
ATCCCCAGCA TGGAGATGTG TGACCAGAGA CACAACATCA CCATGTGTCC TCTGTGTGAC 
AAGACCTGCA GCTACTGGAA GATGAGCTCA GCCTGTGCCA CAGCGCGTGC CAGTCACCTC 
TTTGACAATC CTGCCACTGT CTTCTTTTCT GTGTTTATGG CCCTCTGGGC TGCCACCTTC 
ATGGAGCACT GGAAACGGAA GCAGATGAGA CTCAACTACC GCTGGGACCT CACGGGCTTC 
GAGGAAGAGG AGGAAGCTGT CAAGGATCAT CCCAGAGCAG AGTATGAAGC CAGAGTCTTA 
GAGAAGTCAC TGAGAAAAGA ATCCAGAAAC AAAGAGACTG ACAAGGTGAA GTTGACCTGG 
AGGGACCGAT TCCCAGCCTA TTTCACCAAT CTTGTCTCCA TCATCTTCAT GATCGCAGTG 
ACATTTGCAA TCGTTCTTGG AGTTATCATC TATAGAATCT CCACAGCTGC AGCCTTGGCC 
ATGAACTCCT CCCCATCTGT GCGGTCCAAC ATCCGGGTTA CAGTCACGGC CACTGCTGTC 
ATCATTAACC TCGTGGTCAT CATTCTGCTG GATGAAGTTT ACGGCTGCAT TGCCAGGTGG 
CTCACCAAGA TTGAGGTCCC GAAGACAGAG AAGAGCTTTG AGGAGAGGCT AACCTTCAAG 
GCCTTCCTGC TCAAGTTTGT GAACTCCTAT ACTCCCATCT TCTATGTCGC CTTCTTCAAA 
GGCCGGTTTG TTGGTCGGCC TGGTGACTAC GTTTATATCT TCCGCTCTTT CCGGATGGAG 
GAGTGTGCCC CAGGCGGCTG CCTCATGGAG CTCTGTATCC AGCTGAGCAT CATCATGCTG 
GGCAAGCAGC TAATCCAGAA CAATCTCTTC GAGATCGGCA TCCCGAAGAT GAAAAAATTC 
ATCCGCTACC TGAAGCTGCG CAGGCAGAGC CCCTCGGACC GCGAGGAGTA CATAAAGCGG 
AAGCAGCGCT ATGAGGTGGA CTTCAACCTC GAACCCTTCG CCGGCCTCAC GCCCGAGTAC 
ATGGAAATGA TCATTCAGTT CGGCTTCGTC ACCCTGTTCG TTGCGTCCTT CCCTCTGGCC 
CCACTCTTCG CCCTGCTGAA CAACATCATT GAGATCCGCC TGGATGCCAA AAAGTTTGTC 
ACCGAGCTAC GGAGACCAGT AGCCATCAGA GCCAAAGACA TCGGCATCTG GTATAACATT 
CTCAGAGGTG TCGGGAAGCT GGCTGTCATC ATTAATGCCT TTGTGATCTC CTTCACGTCT 
GACTTCATCC CTCGCCTGGT ATACCTCTAC ATGTACAGTC AAAACGGGAC CATGCACGGC 
TTCGTCAACC ACACGCTCTC CTCCTTCAAT GTCAGCGACT TCCAGAATGG CACAGCACCC 
AATGACCCAC TGGACCTGGG CTACGAGGTT CAGATCTGCA GGTATAAAGA TTACCGGGAG 
CCCCCGTGGT CAGAACACAA GTATGATATC TCCAAAGACT TCTGGGCTGT CCTGGCCGCC 
CGGCTGGCAT TCGTCATTGT CTTCCAGAAC CTGGTGATGT TCATGAGTGA CTTTGTGGAC 
TGGGTGATCC CTGATATCCC CAAAGACATC AGCCAGCAGA TCCACAAGGA GAAGGTTCTC 
ATGGTGGAGT TGTTCATGCG TGAGGAGCAG GGCAAGCAGC AGCTACTGGA CACATGGATG 
GAGAAGGAGA AGCCAAGGGA TGTGCCTTGT AACAACCACA GTCCCACAGC CCACCCAGAG 
GCAGGTGACA GCAGCCCAGT CCCCAGCTAC GAGTACCATG GGGGCGCGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230842.1
CDS	1..3054
Translation	MQDTQDSDISLEGLTREGAPADRECQRGPETIVHEAQDAGTPNSDATGAVDGEREATMRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDVGSPEAPMDYHEDDKRFRREEYEGNLLEAGLELERDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYIKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTAHPEAGDSSPVPSYEYHGGAL

Target ORF information:

RefSeq Version	XM_006230780.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus anoctamin 1 (Ano1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230780.3

ORF Insert Sequence:

ATGCAGGACA CACAGGACAG CGACATCAGC CTGGAGGGCT TGACCCGAGA GGGAGCCCCT 
GCGGACCGAG AGTGCCAGAG GGGACCGGAA ACAATCGTAC ACGAGGCACA GGACGCAGGA 
ACCCCCAACT CAGACGCCAC CGGGGCTGTG GATGGGGAGC GCGAGGCCAC CATGAGGGTC 
CCCGAGAAGT ACTCGACGCT CCCAGCGGAG GACCGCAGCG TCCACATCGT GAACATCTGC 
GCCATCGAGG ACCTGGGCTA CCTGCCGTCC GAGGGCACGT TGCTCAACTC TCTGTCCGTG 
GACCCTGACG CCGAATGCAA GTATGGCCTA TACTTCAGGG ATGGCAAACG CAAGGTGGAC 
TACATCTTGG TGTACCATCA CAAGAGAGCC TCGGGTAGCA GGACTCTGGC CAGGAGAGGA 
CTACAAAATG ACATGGTCCT GGGGACCCGC AGTGTCAGGC AGGACCAGCC CCTTCCCGGG 
AAGGGGAGTC CTGTGGATGT GGGCTCACCG GAAGCCCCCA TGGATTACCA CGAAGATGAC 
AAGCGCTTCA GAAGGGAGGA GTACGAGGGC AACCTGTTGG AGGCAGGCCT GGAGCTGGAA 
CGTGACGAGG ATACCAAAAT CCATGGTGTC GGGTTTGTGA AGATCCATGC GCCCTGGCAT 
GTGCTCTGCA GGGAAGCTGA GTTTTTGAAA CTGAAGATGC CCACGAAGAA GGTGTACCAC 
ATCAGTGAGA CCCGAGGCCT CCTGAAAACC ATCAACTCCG TTCTGCAGAA GATCACAGAC 
CCCATCCAGC CCAAGGTGGC TGAGCACAGA CCACAGACCA CAAAGAGGCT CTCCTACCCC 
TTCTCCAGGG AGAAGCAACA CTTATTCGAC CTGACTGACA GGGACTCTTT TTTCGACAGC 
AAAACCCGGA GCACAATAGT CTATGAAATC CTGAAGAGAA CAACGTGCAC CAAGGCCAAG 
TACAGCATGG GTATCACCAG CCTCCTGGCC AATGGCGTGT ACTCAGCTGC GTACCCTCTG 
CACGATGGTG ACTATGAAGG TGACAACGTT GAGTTCAACG ACAGGAAACT CCTGTATGAG 
GAATGGGCAA GCTATGGAGT CTTCTACAAA TACCAGCCCA TTGACCTGGT CAGGAAATAC 
TTTGGAGAAA AGGTTGGCCT GTACTTTGCC TGGCTTGGAG CCTACACCCA GATGCTCATC 
CCTGCCTCCA TTGTGGGTGT CATTGTCTTT CTCTACGGAT GTGCCACCGT GGACGAAAAC 
ATCCCCAGCA TGGAGATGTG TGACCAGAGA CACAACATCA CCATGTGTCC TCTGTGTGAC 
AAGACCTGCA GCTACTGGAA GATGAGCTCA GCCTGTGCCA CAGCGCGTGC CAGTCACCTC 
TTTGACAATC CTGCCACTGT CTTCTTTTCT GTGTTTATGG CCCTCTGGGC TGCCACCTTC 
ATGGAGCACT GGAAACGGAA GCAGATGAGA CTCAACTACC GCTGGGACCT CACGGGCTTC 
GAGGAAGAGG AGGAAGCTGT CAAGGATCAT CCCAGAGCAG AGTATGAAGC CAGAGTCTTA 
GAGAAGTCAC TGAGAAAAGA ATCCAGAAAC AAAGAGACTG ACAAGGTGAA GTTGACCTGG 
AGGGACCGAT TCCCAGCCTA TTTCACCAAT CTTGTCTCCA TCATCTTCAT GATCGCAGTG 
ACATTTGCAA TCGTTCTTGG AGTTATCATC TATAGAATCT CCACAGCTGC AGCCTTGGCC 
ATGAACTCCT CCCCATCTGT GCGGTCCAAC ATCCGGGTTA CAGTCACGGC CACTGCTGTC 
ATCATTAACC TCGTGGTCAT CATTCTGCTG GATGAAGTTT ACGGCTGCAT TGCCAGGTGG 
CTCACCAAGA TTGAGGTCCC GAAGACAGAG AAGAGCTTTG AGGAGAGGCT AACCTTCAAG 
GCCTTCCTGC TCAAGTTTGT GAACTCCTAT ACTCCCATCT TCTATGTCGC CTTCTTCAAA 
GGCCGGTTTG TTGGTCGGCC TGGTGACTAC GTTTATATCT TCCGCTCTTT CCGGATGGAG 
GAGTGTGCCC CAGGCGGCTG CCTCATGGAG CTCTGTATCC AGCTGAGCAT CATCATGCTG 
GGCAAGCAGC TAATCCAGAA CAATCTCTTC GAGATCGGCA TCCCGAAGAT GAAAAAATTC 
ATCCGCTACC TGAAGCTGCG CAGGCAGAGC CCCTCGGACC GCGAGGAGTA CATAAAGCGG 
AAGCAGCGCT ATGAGGTGGA CTTCAACCTC GAACCCTTCG CCGGCCTCAC GCCCGAGTAC 
ATGGAAATGA TCATTCAGTT CGGCTTCGTC ACCCTGTTCG TTGCGTCCTT CCCTCTGGCC 
CCACTCTTCG CCCTGCTGAA CAACATCATT GAGATCCGCC TGGATGCCAA AAAGTTTGTC 
ACCGAGCTAC GGAGACCAGT AGCCATCAGA GCCAAAGACA TCGGCATCTG GTATAACATT 
CTCAGAGGTG TCGGGAAGCT GGCTGTCATC ATTAATGCCT TTGTGATCTC CTTCACGTCT 
GACTTCATCC CTCGCCTGGT ATACCTCTAC ATGTACAGTC AAAACGGGAC CATGCACGGC 
TTCGTCAACC ACACGCTCTC CTCCTTCAAT GTCAGCGACT TCCAGAATGG CACAGCACCC 
AATGACCCAC TGGACCTGGG CTACGAGGTT CAGATCTGCA GGTATAAAGA TTACCGGGAG 
CCCCCGTGGT CAGAACACAA GTATGATATC TCCAAAGACT TCTGGGCTGT CCTGGCCGCC 
CGGCTGGCAT TCGTCATTGT CTTCCAGAAC CTGGTGATGT TCATGAGTGA CTTTGTGGAC 
TGGGTGATCC CTGATATCCC CAAAGACATC AGCCAGCAGA TCCACAAGGA GAAGGTTCTC 
ATGGTGGAGT TGTTCATGCG TGAGGAGCAG GGCAAGCAGC AGCTACTGGA CACATGGATG 
GAGAAGGAGA AGCCAAGGGA TGTGCCTTGT AACAACCACA GTCCCACAGC CCACCCAGAG 
GCAGGTGACA GCAGCCCAGT CCCCAGCTAC GAGTACCATG GGGGCGCGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TMEM16A and TMEM16B channel proteins generate Ca
The Journal of biological chemistry293(3)995-1006(2018 Jan)
Yamamura H,Nishimura K,Hagihara Y,Suzuki Y,Imaizumi Y

Bile acids stimulate cholangiocyte fluid secretion by activation of membraneTMEM16A Cl
Hepatology (Baltimore, Md.)(2018 Jan)
Li Q,Dutta A,Kresge C,Bugde A,Feranchak AP

Structural basis for anion conduction in the calcium-activated chloride channel TMEM16A.
eLife6(2017 May)
Paulino C,Neldner Y,Lam AK,Kalienkova V,Brunner JD,Schenck S,Dutzler R

ANO1 inhibits cardiac fibrosis after myocardial infraction via TGF-?/smad3 pathway.
Scientific reports7(1)2355(2017 May)
Gao Y,Zhang YM,Qian LJ,Chu M,Hong J,Xu D

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Ano1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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