Sphk2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sphk2
Entrez Gene ID	308589
Full Name	sphingosine kinase 2
General protein information	Preferred Names sphingosine kinase 2 Names sphingosine kinase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_008759384.2	XP_008757606.1	sphingosine kinase 2 isoform X1
XM_006229088.3	XP_006229150.1	sphingosine kinase 2 isoform X1
XM_008759383.2	XP_008757605.1	sphingosine kinase 2 isoform X1
NM_001012066.1	NP_001012066.1	sphingosine kinase 2
XM_006229085.2	XP_006229147.1	sphingosine kinase 2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPHK2	XP_002695244.2
Homo sapiens (human)	SPHK2	NP_064511.2
Pan troglodytes (chimpanzee)	SPHK2	XP_001171404.2
Danio rerio (zebrafish)	sphk2	XP_003200262.2
	SPHK2	XP_001113908.1
Mus musculus (house mouse)	Sphk2	NP_064395.2
Rattus norvegicus (Norway rat)	Sphk2	NP_001012066.1
Drosophila melanogaster (fruit fly)	Sk1	NP_572717.1
Caenorhabditis elegans (roundworm)	sphk-1	NP_001022018.1
Xenopus tropicalis (tropical clawed frog)	sphk2	XP_002938325.1
Canis lupus familiaris (dog)	SPHK2	XP_005616402.1

Gene Ontology
Bibliography

Related articles in PubMed

Hypoxic preconditioning protects cardiomyocytes against hypoxia/reoxygenation-induced cell apoptosis via sphingosine kinase 2 and FAK/AKT pathway.
Zhang R, Li L, Yuan L, Zhao M
Experimental and molecular pathology100(1)51-8(2016 Feb)

Sphingosine kinase 2 and sphingosine-1-phosphate promotes mitochondrial function in dopaminergic neurons of mouse model of Parkinson's disease and in MPP+ -treated MN9D cells in vitro.
Sivasubramanian M, Kanagaraj N, Dheen ST, Tay SS
Neuroscience290636-48(2015 Apr)

Sphingosine 1-phosphate induces filopodia formation through S1PR2 activation of ERM proteins.
Gandy KA, Canals D, Adada M, Wada M, Roddy P, Snider AJ, Hannun YA, Obeid LM
The Biochemical journal449(3)661-72(2013 Feb)

The sphingosine 1-phosphate transporter, SPNS2, functions as a transporter of the phosphorylated form of the immunomodulating agent FTY720.
Hisano Y, Kobayashi N, Kawahara A, Yamaguchi A, Nishi T
The Journal of biological chemistry286(3)1758-66(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

hypoxic preconditioning significantly increased SphK2 expression in primary cardiomyocytes
Title: Hypoxic preconditioning protects cardiomyocytes against hypoxia/reoxygenation-induced cell apoptosis via sphingosine kinase 2 and FAK/AKT pathway.

Glucose-supported beta-cell growth appears to be in part mediated by SPHK activity.
Title: ATP-independent glucose stimulation of sphingosine kinase in rat pancreatic islets.

S1P modulates spinal nociceptive processing through inhibition of neuronal cAMP synthesis and sphingosine kinase 1, but not sphingosine kinase 2
Title: Sphingosine 1-phosphate modulates spinal nociceptive processing.

The following Sphk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sphk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02143	NM_001012066.1 Latest version!	Rattus norvegicus sphingosine kinase 2 (Sphk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa02143	XM_006229085.2 Latest version!	Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa02143	XM_008759383.2 Latest version!	Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa02143	XM_008759384.2 Latest version!	Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa02143	XM_006229088.3 Latest version!	Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sphk2 ( NM_001012066.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012066.1 Rattus norvegicus sphingosine kinase 2 (Sphk2), mRNA.

Price & Availability↑

CloneID	ORa02143
Clone ID Related Accession (Same CDS sequence)	XM_008759384.2 , XM_006229088.3 , XM_008759383.2 , NM_001012066.1 , XM_006229085.2
Accession Version	NM_001012066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079120.1. On Feb 11, 2005 this sequence version replaced XM_218558.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC079120.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642782 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012066.1
CDS	97..1947
Translation	MAPPPLLPVAASTPILHGEFGSYPAHGPRFALTLTTQALHIQRLRPKPEARPRDGLVPLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGAATYVENRAEAQRWATALMCLLRGVPLSGDQEITPELLPRKPRLLLLVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQTVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLATLHTYRGRLSYLPATTEPALPIPGHGLPRAKSELALAPAPAPAATHSPLHRSVSDLPLPLPQPALASPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNAFKTAQLSPIAEGPSEMPASGLLPPIHNAPEASTWGPADHLLPPLGSPLPQDWVTIEGEFVLMLAILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGVLTVDGELVEYGPIQAQVHPSLATLLTGPAGQKPRA

Target ORF information:

RefSeq Version	NM_001012066.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 2 (Sphk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012066.1

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( XM_008759384.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757606.1 Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa02143
Clone ID Related Accession (Same CDS sequence)	XM_008759384.2 , XM_006229088.3 , XM_008759383.2 , NM_001012066.1 , XM_006229085.2
Accession Version	XM_008759384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757606.1
CDS	528..2378
Translation	MAPPPLLPVAASTPILHGEFGSYPAHGPRFALTLTTQALHIQRLRPKPEARPRDGLVPLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGAATYVENRAEAQRWATALMCLLRGVPLSGDQEITPELLPRKPRLLLLVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQTVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLATLHTYRGRLSYLPATTEPALPIPGHGLPRAKSELALAPAPAPAATHSPLHRSVSDLPLPLPQPALASPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNAFKTAQLSPIAEGPSEMPASGLLPPIHNAPEASTWGPADHLLPPLGSPLPQDWVTIEGEFVLMLAILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGVLTVDGELVEYGPIQAQVHPSLATLLTGPAGQKPRA

Target ORF information:

RefSeq Version	XM_008759384.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759384.2

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( XM_006229088.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229150.1 Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa02143
Clone ID Related Accession (Same CDS sequence)	XM_008759384.2 , XM_006229088.3 , XM_008759383.2 , NM_001012066.1 , XM_006229085.2
Accession Version	XM_006229088.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229150.1
CDS	246..2096
Translation	MAPPPLLPVAASTPILHGEFGSYPAHGPRFALTLTTQALHIQRLRPKPEARPRDGLVPLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGAATYVENRAEAQRWATALMCLLRGVPLSGDQEITPELLPRKPRLLLLVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQTVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLATLHTYRGRLSYLPATTEPALPIPGHGLPRAKSELALAPAPAPAATHSPLHRSVSDLPLPLPQPALASPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNAFKTAQLSPIAEGPSEMPASGLLPPIHNAPEASTWGPADHLLPPLGSPLPQDWVTIEGEFVLMLAILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGVLTVDGELVEYGPIQAQVHPSLATLLTGPAGQKPRA

Target ORF information:

RefSeq Version	XM_006229088.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229088.3

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( XM_008759383.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757605.1 Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X2, mRNA.

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CloneID	ORa02143
Clone ID Related Accession (Same CDS sequence)	XM_008759384.2 , XM_006229088.3 , XM_008759383.2 , NM_001012066.1 , XM_006229085.2
Accession Version	XM_008759383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757605.1
CDS	225..2075
Translation	MAPPPLLPVAASTPILHGEFGSYPAHGPRFALTLTTQALHIQRLRPKPEARPRDGLVPLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGAATYVENRAEAQRWATALMCLLRGVPLSGDQEITPELLPRKPRLLLLVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQTVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLATLHTYRGRLSYLPATTEPALPIPGHGLPRAKSELALAPAPAPAATHSPLHRSVSDLPLPLPQPALASPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNAFKTAQLSPIAEGPSEMPASGLLPPIHNAPEASTWGPADHLLPPLGSPLPQDWVTIEGEFVLMLAILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGVLTVDGELVEYGPIQAQVHPSLATLLTGPAGQKPRA

Target ORF information:

RefSeq Version	XM_008759383.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759383.2

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( XM_006229085.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229147.1 Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02143
Clone ID Related Accession (Same CDS sequence)	XM_008759384.2 , XM_006229088.3 , XM_008759383.2 , NM_001012066.1 , XM_006229085.2
Accession Version	XM_006229085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229147.1
CDS	186..2036
Translation	MAPPPLLPVAASTPILHGEFGSYPAHGPRFALTLTTQALHIQRLRPKPEARPRDGLVPLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGAATYVENRAEAQRWATALMCLLRGVPLSGDQEITPELLPRKPRLLLLVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQTVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLATLHTYRGRLSYLPATTEPALPIPGHGLPRAKSELALAPAPAPAATHSPLHRSVSDLPLPLPQPALASPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNAFKTAQLSPIAEGPSEMPASGLLPPIHNAPEASTWGPADHLLPPLGSPLPQDWVTIEGEFVLMLAILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGVLTVDGELVEYGPIQAQVHPSLATLLTGPAGQKPRA

Target ORF information:

RefSeq Version	XM_006229085.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229085.2

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CGATCCTGCA CGGCGAGTTT 
GGTTCCTACC CAGCCCATGG CCCCAGGTTT GCCCTCACCC TCACGACACA AGCCCTACAC 
ATACAGCGAC TGCGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CCCTCTGGAC 
GAGGTCTCAG GCTGTGGCAC TCTGCAGAGC CGTAGCCCCG AAGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGGCGT GGGGGCCGGC GCAGAGCTAC TCGTACCTTC 
CGGGCCGACG GGGCCGCCAC TTATGTGGAG AATCGTGCAG AGGCCCAGCG TTGGGCAACT 
GCTCTCATGT GTCTCCTCCG AGGAGTGCCT CTGTCTGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCCTA TTGGTCAATC CTTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCCATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAAAACCAT GCACGTGAGC TGGTGCAGGG GTTGAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCC GGAGATGGGC TGCTTTATGA GGTGCTGAAT 
GGGCTCCTCG ACCGCCCAGA TTGGGAAGAC GCTGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGTTCGG GCAATGCACT AGCTGGGGCA GTTAACCATC ATGGCGGGTT TGAGCAAACT 
GTTGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGGGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCA GGATCCCGCT GTTTTTCCTT CCTGTCAGTT 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGCGAGC GCTTCAGAGC CCTGGGCAGT 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCACAT TGCATACCTA CCGTGGACGT 
CTCTCCTACC TCCCTGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA TGGTCTGCCA 
AGAGCCAAGT CAGAACTAGC CTTGGCCCCA GCCCCAGCCC CAGCCGCCAC CCACTCACCT 
CTTCATCGAT CTGTGTCTGA CCTGCCCCTG CCTCTTCCCC AGCCTGCCCT GGCCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGGG GAGCTGGGGA TGCACCTTTG TCCCCAGACC CATTGCTGCC TTCATCCCCC 
AACGCTTTCA AAACAGCTCA GCTTTCACCC ATCGCCGAAG GGCCCTCAGA AATGCCAGCA 
TCAGGGCTGC TGCCTCCCAT CCACAATGCT CCAGAAGCCT CTACCTGGGG CCCAGCGGAC 
CACCTGCTCC CTCCCCTGGG CTCTCCACTG CCCCAAGACT GGGTGACAAT AGAGGGGGAG 
TTTGTACTCA TGTTGGCCAT CTTGCCAAGC CACCTCTGCG CAGACCTGAT GGCAGCCCCA 
CACGCGCGTT TTGACGATGG TGTGGTGCAC CTGTGCTGGG TGCGGAGCGG CATCTCACGG 
GCCGCACTTC TACGAATTTT GCTGGCCATG GAACATGGAA ACCACTTCAG CCTGGGCTGC 
CCACATCTGG GCTATGCTGC AGCACGTGCC TTCCGCCTTG AGCCACTCAC GCCTCGTGGG 
GTGCTCACTG TAGATGGGGA ATTAGTAGAG TATGGGCCAA TACAGGCACA GGTGCATCCA 
AGTCTCGCCA CACTGCTCAC TGGGCCTGCA GGTCAAAAGC CTCGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypoxic preconditioning protects cardiomyocytes against hypoxia/reoxygenation-induced cell apoptosis via sphingosine kinase 2 and FAK/AKT pathway.
Experimental and molecular pathology100(1)51-8(2016 Feb)
Zhang R,Li L,Yuan L,Zhao M

Sphingosine kinase 2 and sphingosine-1-phosphate promotes mitochondrial function in dopaminergic neurons of mouse model of Parkinson's disease and in MPP+ -treated MN9D cells in vitro.
Neuroscience290636-48(2015 Apr)
Sivasubramanian M,Kanagaraj N,Dheen ST,Tay SS

Sphingosine 1-phosphate induces filopodia formation through S1PR2 activation of ERM proteins.
The Biochemical journal449(3)661-72(2013 Feb)
Gandy KA,Canals D,Adada M,Wada M,Roddy P,Snider AJ,Hannun YA,Obeid LM

The sphingosine 1-phosphate transporter, SPNS2, functions as a transporter of the phosphorylated form of the immunomodulating agent FTY720.
The Journal of biological chemistry286(3)1758-66(2011 Jan)
Hisano Y,Kobayashi N,Kawahara A,Yamaguchi A,Nishi T

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Sphk2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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