Iars1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Iars1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Iars1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa34934 NM_001100572.1
Latest version!
Rattus norvegicus isoleucyl-tRNA synthetase 1 (Iars1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa34934 XM_006253706.3
Latest version!
Rattus norvegicus isoleucyl-tRNA synthetase (Iars), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa34934 XM_008771533.2
Latest version!
Rattus norvegicus isoleucyl-tRNA synthetase (Iars), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa34934
    Clone ID Related Accession (Same CDS sequence) XM_008771533.2 , XM_006253706.3 , NM_001100572.1
    Accession Version NM_001100572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-12
    Organism Rattus norvegicus(Norway rat)
    Product isoleucine--tRNA ligase, cytoplasmic
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07072539.1. On or before Oct 3, 2008 this sequence version replaced XM_001055989.1, XM_225196.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCGGA AAACATCAGC TTTCCTGCTG AAGAAGAAAA AATCTTACAG 
    TTCTGGTCCA AATACAACTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGCT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTCGCT ACTGGACTGC CTCACTACGG ACACATACTT
    GCGGGGACAA TTAAAGATAT AGTTACAAGA TACGCTCACC AGAGCGGGTT TCACGTCGAC
    AGAAGATTCG GGTGGGATTG TCATGGCTTA CCTGTGGAGT ACGAGATTGA CAAGACCCTG
    GGGATCAAAG GGCCAGAGGA CGTGGCCAAG ATGGGGATCG CAGAGTACAA CAAGCAGTGC
    CGAGCTATTG TGATGAGGTA CTCCGCCGAG TGGAAGTCTA CTGTTACCAG GCTTGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACC CTGTACCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA AGTCATGCCC
    TTCTCCACCG CGTGTGGAAC TCCACTCTCC AACTTCGAGT CGAACCAGAA TTACAAGGAC
    GTTCAGGACC CTTCAGTATT TGTAACGTTC CCTTTGGAGG AAGATGAAAA CACATCCTTA
    GTTGCTTGGA CAACCACTCC GTGGACCCTA CCCAGTAACC TTGCGCTATG TGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGACGTT TCCAGGGGGA AGTTGTTCAT TTTGATGGAA
    GCCAGATTGT CGGCCCTCTA TAAACAAGAG AGCGACTATG AGATCCTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTTGAC CACTATGTGA AGGATGAAGA AGGCACAGGT
    GTCGTTCACC AGGCTCCTTA CTTTGGCGCT GACGATCATC GAGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCAGC CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTCGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATCAGGATG
    TTGAAGGAGC AGGGCCGACT GCTCGTGGCA AGCACCTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCGG ACACTCCCCT CATTTACAAG TCCGTGCCCA GCTGGTTCGT GCGGGTGGAG
    CACATGGTGG ACCAGCTACT GAAGAACAAT GATCTGTGTT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCACGTG ACTGGGCAAT TTCCCGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGTGAAG ACTTTGAGGA GGTAGTGTGC
    ATCGGGTCAG TGGCAGAACT TGAAGAATTG TCAGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATACCATCC CGCTGCGGGA AAGGACCGTT GCGGCGTGTC
    TCTGAGGTGT TTGACTGTTG GTTCGAGAGT GGAAGTATGC CTTACGCCCA GGTTCATTAC
    CCCTTTGAGA GCAAGCGAGA GTTCGAGGAT GCCTTTCCCG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTAGTGC TAGCCACAGC CCTCTTTGGA
    CAACCACCTT TCAAGAACGT GATTGTGAAC GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA CTACCCAGAT CCGGTTTCCA TCATCAATAA GTACGGTGCC
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTGA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GCGTACGGGA TGTCCTCAAA GACGTGCTGC TCCCATGGTA CAACGCATAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTG CACAAGGAAG AAGAAGTAGA GTTTCTGTAC
    AACGAGCACA CGGTCCGAGA AAGCCCCAAC ATCACCGACC GGTGGATTCT GTCCTTCATG
    CAGTCGCTCA TTGCGTTCTT TGAGACGGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTG
    CCTCGCCTGG TCAAGTTCGT AGATATTCTG ACCAACTGGT ACGTCCGAAT GAACAGGAGA
    AGATTAAAGG GGGAGAATGG CATGGAAGAC TGTGTTATGG CTCTGGAGAC TCTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACAC CCTTTCTTAC TGAACTGATG
    TACCAGAACC TGAAGCTGCT GATTGACCCT GCATCTATCC GGGACAAGGA CACCCTCAGC
    ATCCACTACC TCATGCTGCC TCATGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GAATGCAGTC CGTGATTGAG CTTGGGAGAG TGATTCGAGA CCGGAAAACT
    ATTCCAATAA AGTATCCCTT GAAGGAAATT GTCGTCATCC ACCAAGACCC AGAAGCTCTT
    GAGGATATCA AGTCCTTGGA GAAGTATATC ATTGAGGAAC TAAATGTTCG AAAAGTTACA
    CTGTCTACTG ATAAAAACAA GTATGGCATT CGACTGAGGG CTGAGCCAGA TCACATGGTC
    CTTGGGAAAC GTCTGAAGGG AGCCTTTAAA GCAGTGATGG TGGCCGTAAA GCAGCTGAGC
    AATGAGGAGC TGGAGCAGTT TCAGAAGAGT GGGAGCATCG TCGTGGAAGG CCATGAATTA
    CACGAAGAAG ATATTCGTCT CATGTACACC TTTGACCAGG CCACAGGAGG CACTGCACAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTTTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGACG AAGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGCAAGAAG
    TGCAACCTGG TTCCGACGGA TGAAATAACA GTTTACTATA ATGCCAAATC TGAAGGAAGA
    TACTTGAGTA ATGTCATCGA AAGCCACACT GACTTCATAT ATGCCACTAT CAAAGCTCCC
    TTGAAGCCCT ATCCAGTTCC TACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTACG AGCTGGAAAT CACGATCACC AGAGGATCCT GTGTGCCTGG GCCCGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACTGAGC AAGGTGGAGT GTTACTCCTG
    GAGAATCCGA AGGGTGATAA TCAGTTGAAC CTTGAGAAGC TGAAGAGCGT CATTACCAGC
    ATATTCGGTG TTAAAAGCAC AGGGCTGTCA GTCTTCAACG GTGGAACAGA ATTACAGAAC
    CAGACTGACT TGCTGAGCCT CAGTGGAAGG ACGCTGTGTG TGACTGCAGG AGCATCCCTG
    GCTCCAGCCA GCAGCCCCAG CACCCTTCTC TGTCAGTACA TCAACCTGCA GCTCGTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTT
    GGACAGAACG GGCTCACCCA CCAAGGGCTT GTGTACGAAG CAGCCAAGGT GTTTGGACTG
    CGCAGCCGGA GGCTAAAGCT GTTCCTGAAT GAGACTCAGA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACGACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001094042.1
    CDS211..3999
    Translation

    Target ORF information:

    RefSeq Version NM_001100572.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus isoleucyl-tRNA synthetase 1 (Iars1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001100572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCGGA AAACATCAGC TTTCCTGCTG AAGAAGAAAA AATCTTACAG 
    TTCTGGTCCA AATACAACTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGCT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTCGCT ACTGGACTGC CTCACTACGG ACACATACTT
    GCGGGGACAA TTAAAGATAT AGTTACAAGA TACGCTCACC AGAGCGGGTT TCACGTCGAC
    AGAAGATTCG GGTGGGATTG TCATGGCTTA CCTGTGGAGT ACGAGATTGA CAAGACCCTG
    GGGATCAAAG GGCCAGAGGA CGTGGCCAAG ATGGGGATCG CAGAGTACAA CAAGCAGTGC
    CGAGCTATTG TGATGAGGTA CTCCGCCGAG TGGAAGTCTA CTGTTACCAG GCTTGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACC CTGTACCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA AGTCATGCCC
    TTCTCCACCG CGTGTGGAAC TCCACTCTCC AACTTCGAGT CGAACCAGAA TTACAAGGAC
    GTTCAGGACC CTTCAGTATT TGTAACGTTC CCTTTGGAGG AAGATGAAAA CACATCCTTA
    GTTGCTTGGA CAACCACTCC GTGGACCCTA CCCAGTAACC TTGCGCTATG TGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGACGTT TCCAGGGGGA AGTTGTTCAT TTTGATGGAA
    GCCAGATTGT CGGCCCTCTA TAAACAAGAG AGCGACTATG AGATCCTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTTGAC CACTATGTGA AGGATGAAGA AGGCACAGGT
    GTCGTTCACC AGGCTCCTTA CTTTGGCGCT GACGATCATC GAGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCAGC CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTCGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATCAGGATG
    TTGAAGGAGC AGGGCCGACT GCTCGTGGCA AGCACCTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCGG ACACTCCCCT CATTTACAAG TCCGTGCCCA GCTGGTTCGT GCGGGTGGAG
    CACATGGTGG ACCAGCTACT GAAGAACAAT GATCTGTGTT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCACGTG ACTGGGCAAT TTCCCGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGTGAAG ACTTTGAGGA GGTAGTGTGC
    ATCGGGTCAG TGGCAGAACT TGAAGAATTG TCAGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATACCATCC CGCTGCGGGA AAGGACCGTT GCGGCGTGTC
    TCTGAGGTGT TTGACTGTTG GTTCGAGAGT GGAAGTATGC CTTACGCCCA GGTTCATTAC
    CCCTTTGAGA GCAAGCGAGA GTTCGAGGAT GCCTTTCCCG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTAGTGC TAGCCACAGC CCTCTTTGGA
    CAACCACCTT TCAAGAACGT GATTGTGAAC GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA CTACCCAGAT CCGGTTTCCA TCATCAATAA GTACGGTGCC
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTGA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GCGTACGGGA TGTCCTCAAA GACGTGCTGC TCCCATGGTA CAACGCATAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTG CACAAGGAAG AAGAAGTAGA GTTTCTGTAC
    AACGAGCACA CGGTCCGAGA AAGCCCCAAC ATCACCGACC GGTGGATTCT GTCCTTCATG
    CAGTCGCTCA TTGCGTTCTT TGAGACGGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTG
    CCTCGCCTGG TCAAGTTCGT AGATATTCTG ACCAACTGGT ACGTCCGAAT GAACAGGAGA
    AGATTAAAGG GGGAGAATGG CATGGAAGAC TGTGTTATGG CTCTGGAGAC TCTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACAC CCTTTCTTAC TGAACTGATG
    TACCAGAACC TGAAGCTGCT GATTGACCCT GCATCTATCC GGGACAAGGA CACCCTCAGC
    ATCCACTACC TCATGCTGCC TCATGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GAATGCAGTC CGTGATTGAG CTTGGGAGAG TGATTCGAGA CCGGAAAACT
    ATTCCAATAA AGTATCCCTT GAAGGAAATT GTCGTCATCC ACCAAGACCC AGAAGCTCTT
    GAGGATATCA AGTCCTTGGA GAAGTATATC ATTGAGGAAC TAAATGTTCG AAAAGTTACA
    CTGTCTACTG ATAAAAACAA GTATGGCATT CGACTGAGGG CTGAGCCAGA TCACATGGTC
    CTTGGGAAAC GTCTGAAGGG AGCCTTTAAA GCAGTGATGG TGGCCGTAAA GCAGCTGAGC
    AATGAGGAGC TGGAGCAGTT TCAGAAGAGT GGGAGCATCG TCGTGGAAGG CCATGAATTA
    CACGAAGAAG ATATTCGTCT CATGTACACC TTTGACCAGG CCACAGGAGG CACTGCACAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTTTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGACG AAGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGCAAGAAG
    TGCAACCTGG TTCCGACGGA TGAAATAACA GTTTACTATA ATGCCAAATC TGAAGGAAGA
    TACTTGAGTA ATGTCATCGA AAGCCACACT GACTTCATAT ATGCCACTAT CAAAGCTCCC
    TTGAAGCCCT ATCCAGTTCC TACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTACG AGCTGGAAAT CACGATCACC AGAGGATCCT GTGTGCCTGG GCCCGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACTGAGC AAGGTGGAGT GTTACTCCTG
    GAGAATCCGA AGGGTGATAA TCAGTTGAAC CTTGAGAAGC TGAAGAGCGT CATTACCAGC
    ATATTCGGTG TTAAAAGCAC AGGGCTGTCA GTCTTCAACG GTGGAACAGA ATTACAGAAC
    CAGACTGACT TGCTGAGCCT CAGTGGAAGG ACGCTGTGTG TGACTGCAGG AGCATCCCTG
    GCTCCAGCCA GCAGCCCCAG CACCCTTCTC TGTCAGTACA TCAACCTGCA GCTCGTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTT
    GGACAGAACG GGCTCACCCA CCAAGGGCTT GTGTACGAAG CAGCCAAGGT GTTTGGACTG
    CGCAGCCGGA GGCTAAAGCT GTTCCTGAAT GAGACTCAGA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACGACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa34934
    Clone ID Related Accession (Same CDS sequence) XM_008771533.2 , XM_006253706.3 , NM_001100572.1
    Accession Version XM_008771533.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCGGA AAACATCAGC TTTCCTGCTG AAGAAGAAAA AATCTTACAG 
    TTCTGGTCCA AATACAACTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGCT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTCGCT ACTGGACTGC CTCACTACGG ACACATACTT
    GCGGGGACAA TTAAAGATAT AGTTACAAGA TACGCTCACC AGAGCGGGTT TCACGTCGAC
    AGAAGATTCG GGTGGGATTG TCATGGCTTA CCTGTGGAGT ACGAGATTGA CAAGACCCTG
    GGGATCAAAG GGCCAGAGGA CGTGGCCAAG ATGGGGATCG CAGAGTACAA CAAGCAGTGC
    CGAGCTATTG TGATGAGGTA CTCCGCCGAG TGGAAGTCTA CTGTTACCAG GCTTGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACC CTGTACCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA AGTCATGCCC
    TTCTCCACCG CGTGTGGAAC TCCACTCTCC AACTTCGAGT CGAACCAGAA TTACAAGGAC
    GTTCAGGACC CTTCAGTATT TGTAACGTTC CCTTTGGAGG AAGATGAAAA CACATCCTTA
    GTTGCTTGGA CAACCACTCC GTGGACCCTA CCCAGTAACC TTGCGCTATG TGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGACGTT TCCAGGGGGA AGTTGTTCAT TTTGATGGAA
    GCCAGATTGT CGGCCCTCTA TAAACAAGAG AGCGACTATG AGATCCTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTTGAC CACTATGTGA AGGATGAAGA AGGCACAGGT
    GTCGTTCACC AGGCTCCTTA CTTTGGCGCT GACGATCATC GAGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCAGC CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTCGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATCAGGATG
    TTGAAGGAGC AGGGCCGACT GCTCGTGGCA AGCACCTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCGG ACACTCCCCT CATTTACAAG TCCGTGCCCA GCTGGTTCGT GCGGGTGGAG
    CACATGGTGG ACCAGCTACT GAAGAACAAT GATCTGTGTT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCACGTG ACTGGGCAAT TTCCCGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGTGAAG ACTTTGAGGA GGTAGTGTGC
    ATCGGGTCAG TGGCAGAACT TGAAGAATTG TCAGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATACCATCC CGCTGCGGGA AAGGACCGTT GCGGCGTGTC
    TCTGAGGTGT TTGACTGTTG GTTCGAGAGT GGAAGTATGC CTTACGCCCA GGTTCATTAC
    CCCTTTGAGA GCAAGCGAGA GTTCGAGGAT GCCTTTCCCG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTAGTGC TAGCCACAGC CCTCTTTGGA
    CAACCACCTT TCAAGAACGT GATTGTGAAC GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA CTACCCAGAT CCGGTTTCCA TCATCAATAA GTACGGTGCC
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTGA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GCGTACGGGA TGTCCTCAAA GACGTGCTGC TCCCATGGTA CAACGCATAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTG CACAAGGAAG AAGAAGTAGA GTTTCTGTAC
    AACGAGCACA CGGTCCGAGA AAGCCCCAAC ATCACCGACC GGTGGATTCT GTCCTTCATG
    CAGTCGCTCA TTGCGTTCTT TGAGACGGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTG
    CCTCGCCTGG TCAAGTTCGT AGATATTCTG ACCAACTGGT ACGTCCGAAT GAACAGGAGA
    AGATTAAAGG GGGAGAATGG CATGGAAGAC TGTGTTATGG CTCTGGAGAC TCTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACAC CCTTTCTTAC TGAACTGATG
    TACCAGAACC TGAAGCTGCT GATTGACCCT GCATCTATCC GGGACAAGGA CACCCTCAGC
    ATCCACTACC TCATGCTGCC TCATGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GAATGCAGTC CGTGATTGAG CTTGGGAGAG TGATTCGAGA CCGGAAAACT
    ATTCCAATAA AGTATCCCTT GAAGGAAATT GTCGTCATCC ACCAAGACCC AGAAGCTCTT
    GAGGATATCA AGTCCTTGGA GAAGTATATC ATTGAGGAAC TAAATGTTCG AAAAGTTACA
    CTGTCTACTG ATAAAAACAA GTATGGCATT CGACTGAGGG CTGAGCCAGA TCACATGGTC
    CTTGGGAAAC GTCTGAAGGG AGCCTTTAAA GCAGTGATGG TGGCCGTAAA GCAGCTGAGC
    AATGAGGAGC TGGAGCAGTT TCAGAAGAGT GGGAGCATCG TCGTGGAAGG CCATGAATTA
    CACGAAGAAG ATATTCGTCT CATGTACACC TTTGACCAGG CCACAGGAGG CACTGCACAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTTTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGACG AAGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGCAAGAAG
    TGCAACCTGG TTCCGACGGA TGAAATAACA GTTTACTATA ATGCCAAATC TGAAGGAAGA
    TACTTGAGTA ATGTCATCGA AAGCCACACT GACTTCATAT ATGCCACTAT CAAAGCTCCC
    TTGAAGCCCT ATCCAGTTCC TACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTACG AGCTGGAAAT CACGATCACC AGAGGATCCT GTGTGCCTGG GCCCGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACTGAGC AAGGTGGAGT GTTACTCCTG
    GAGAATCCGA AGGGTGATAA TCAGTTGAAC CTTGAGAAGC TGAAGAGCGT CATTACCAGC
    ATATTCGGTG TTAAAAGCAC AGGGCTGTCA GTCTTCAACG GTGGAACAGA ATTACAGAAC
    CAGACTGACT TGCTGAGCCT CAGTGGAAGG ACGCTGTGTG TGACTGCAGG AGCATCCCTG
    GCTCCAGCCA GCAGCCCCAG CACCCTTCTC TGTCAGTACA TCAACCTGCA GCTCGTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTT
    GGACAGAACG GGCTCACCCA CCAAGGGCTT GTGTACGAAG CAGCCAAGGT GTTTGGACTG
    CGCAGCCGGA GGCTAAAGCT GTTCCTGAAT GAGACTCAGA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACGACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008769755.1
    CDS316..4104
    Translation

    Target ORF information:

    RefSeq Version XM_008771533.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus isoleucyl-tRNA synthetase (Iars), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008771533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCGGA AAACATCAGC TTTCCTGCTG AAGAAGAAAA AATCTTACAG 
    TTCTGGTCCA AATACAACTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGCT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTCGCT ACTGGACTGC CTCACTACGG ACACATACTT
    GCGGGGACAA TTAAAGATAT AGTTACAAGA TACGCTCACC AGAGCGGGTT TCACGTCGAC
    AGAAGATTCG GGTGGGATTG TCATGGCTTA CCTGTGGAGT ACGAGATTGA CAAGACCCTG
    GGGATCAAAG GGCCAGAGGA CGTGGCCAAG ATGGGGATCG CAGAGTACAA CAAGCAGTGC
    CGAGCTATTG TGATGAGGTA CTCCGCCGAG TGGAAGTCTA CTGTTACCAG GCTTGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACC CTGTACCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA AGTCATGCCC
    TTCTCCACCG CGTGTGGAAC TCCACTCTCC AACTTCGAGT CGAACCAGAA TTACAAGGAC
    GTTCAGGACC CTTCAGTATT TGTAACGTTC CCTTTGGAGG AAGATGAAAA CACATCCTTA
    GTTGCTTGGA CAACCACTCC GTGGACCCTA CCCAGTAACC TTGCGCTATG TGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGACGTT TCCAGGGGGA AGTTGTTCAT TTTGATGGAA
    GCCAGATTGT CGGCCCTCTA TAAACAAGAG AGCGACTATG AGATCCTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTTGAC CACTATGTGA AGGATGAAGA AGGCACAGGT
    GTCGTTCACC AGGCTCCTTA CTTTGGCGCT GACGATCATC GAGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCAGC CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTCGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATCAGGATG
    TTGAAGGAGC AGGGCCGACT GCTCGTGGCA AGCACCTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCGG ACACTCCCCT CATTTACAAG TCCGTGCCCA GCTGGTTCGT GCGGGTGGAG
    CACATGGTGG ACCAGCTACT GAAGAACAAT GATCTGTGTT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCACGTG ACTGGGCAAT TTCCCGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGTGAAG ACTTTGAGGA GGTAGTGTGC
    ATCGGGTCAG TGGCAGAACT TGAAGAATTG TCAGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATACCATCC CGCTGCGGGA AAGGACCGTT GCGGCGTGTC
    TCTGAGGTGT TTGACTGTTG GTTCGAGAGT GGAAGTATGC CTTACGCCCA GGTTCATTAC
    CCCTTTGAGA GCAAGCGAGA GTTCGAGGAT GCCTTTCCCG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTAGTGC TAGCCACAGC CCTCTTTGGA
    CAACCACCTT TCAAGAACGT GATTGTGAAC GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA CTACCCAGAT CCGGTTTCCA TCATCAATAA GTACGGTGCC
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTGA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GCGTACGGGA TGTCCTCAAA GACGTGCTGC TCCCATGGTA CAACGCATAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTG CACAAGGAAG AAGAAGTAGA GTTTCTGTAC
    AACGAGCACA CGGTCCGAGA AAGCCCCAAC ATCACCGACC GGTGGATTCT GTCCTTCATG
    CAGTCGCTCA TTGCGTTCTT TGAGACGGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTG
    CCTCGCCTGG TCAAGTTCGT AGATATTCTG ACCAACTGGT ACGTCCGAAT GAACAGGAGA
    AGATTAAAGG GGGAGAATGG CATGGAAGAC TGTGTTATGG CTCTGGAGAC TCTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACAC CCTTTCTTAC TGAACTGATG
    TACCAGAACC TGAAGCTGCT GATTGACCCT GCATCTATCC GGGACAAGGA CACCCTCAGC
    ATCCACTACC TCATGCTGCC TCATGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GAATGCAGTC CGTGATTGAG CTTGGGAGAG TGATTCGAGA CCGGAAAACT
    ATTCCAATAA AGTATCCCTT GAAGGAAATT GTCGTCATCC ACCAAGACCC AGAAGCTCTT
    GAGGATATCA AGTCCTTGGA GAAGTATATC ATTGAGGAAC TAAATGTTCG AAAAGTTACA
    CTGTCTACTG ATAAAAACAA GTATGGCATT CGACTGAGGG CTGAGCCAGA TCACATGGTC
    CTTGGGAAAC GTCTGAAGGG AGCCTTTAAA GCAGTGATGG TGGCCGTAAA GCAGCTGAGC
    AATGAGGAGC TGGAGCAGTT TCAGAAGAGT GGGAGCATCG TCGTGGAAGG CCATGAATTA
    CACGAAGAAG ATATTCGTCT CATGTACACC TTTGACCAGG CCACAGGAGG CACTGCACAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTTTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGACG AAGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGCAAGAAG
    TGCAACCTGG TTCCGACGGA TGAAATAACA GTTTACTATA ATGCCAAATC TGAAGGAAGA
    TACTTGAGTA ATGTCATCGA AAGCCACACT GACTTCATAT ATGCCACTAT CAAAGCTCCC
    TTGAAGCCCT ATCCAGTTCC TACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTACG AGCTGGAAAT CACGATCACC AGAGGATCCT GTGTGCCTGG GCCCGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACTGAGC AAGGTGGAGT GTTACTCCTG
    GAGAATCCGA AGGGTGATAA TCAGTTGAAC CTTGAGAAGC TGAAGAGCGT CATTACCAGC
    ATATTCGGTG TTAAAAGCAC AGGGCTGTCA GTCTTCAACG GTGGAACAGA ATTACAGAAC
    CAGACTGACT TGCTGAGCCT CAGTGGAAGG ACGCTGTGTG TGACTGCAGG AGCATCCCTG
    GCTCCAGCCA GCAGCCCCAG CACCCTTCTC TGTCAGTACA TCAACCTGCA GCTCGTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTT
    GGACAGAACG GGCTCACCCA CCAAGGGCTT GTGTACGAAG CAGCCAAGGT GTTTGGACTG
    CGCAGCCGGA GGCTAAAGCT GTTCCTGAAT GAGACTCAGA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACGACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa34934
    Clone ID Related Accession (Same CDS sequence) XM_008771533.2 , XM_006253706.3 , NM_001100572.1
    Accession Version XM_006253706.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCGGA AAACATCAGC TTTCCTGCTG AAGAAGAAAA AATCTTACAG 
    TTCTGGTCCA AATACAACTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGCT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTCGCT ACTGGACTGC CTCACTACGG ACACATACTT
    GCGGGGACAA TTAAAGATAT AGTTACAAGA TACGCTCACC AGAGCGGGTT TCACGTCGAC
    AGAAGATTCG GGTGGGATTG TCATGGCTTA CCTGTGGAGT ACGAGATTGA CAAGACCCTG
    GGGATCAAAG GGCCAGAGGA CGTGGCCAAG ATGGGGATCG CAGAGTACAA CAAGCAGTGC
    CGAGCTATTG TGATGAGGTA CTCCGCCGAG TGGAAGTCTA CTGTTACCAG GCTTGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACC CTGTACCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA AGTCATGCCC
    TTCTCCACCG CGTGTGGAAC TCCACTCTCC AACTTCGAGT CGAACCAGAA TTACAAGGAC
    GTTCAGGACC CTTCAGTATT TGTAACGTTC CCTTTGGAGG AAGATGAAAA CACATCCTTA
    GTTGCTTGGA CAACCACTCC GTGGACCCTA CCCAGTAACC TTGCGCTATG TGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGACGTT TCCAGGGGGA AGTTGTTCAT TTTGATGGAA
    GCCAGATTGT CGGCCCTCTA TAAACAAGAG AGCGACTATG AGATCCTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTTGAC CACTATGTGA AGGATGAAGA AGGCACAGGT
    GTCGTTCACC AGGCTCCTTA CTTTGGCGCT GACGATCATC GAGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCAGC CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTCGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATCAGGATG
    TTGAAGGAGC AGGGCCGACT GCTCGTGGCA AGCACCTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCGG ACACTCCCCT CATTTACAAG TCCGTGCCCA GCTGGTTCGT GCGGGTGGAG
    CACATGGTGG ACCAGCTACT GAAGAACAAT GATCTGTGTT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCACGTG ACTGGGCAAT TTCCCGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGTGAAG ACTTTGAGGA GGTAGTGTGC
    ATCGGGTCAG TGGCAGAACT TGAAGAATTG TCAGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATACCATCC CGCTGCGGGA AAGGACCGTT GCGGCGTGTC
    TCTGAGGTGT TTGACTGTTG GTTCGAGAGT GGAAGTATGC CTTACGCCCA GGTTCATTAC
    CCCTTTGAGA GCAAGCGAGA GTTCGAGGAT GCCTTTCCCG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTAGTGC TAGCCACAGC CCTCTTTGGA
    CAACCACCTT TCAAGAACGT GATTGTGAAC GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA CTACCCAGAT CCGGTTTCCA TCATCAATAA GTACGGTGCC
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTGA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GCGTACGGGA TGTCCTCAAA GACGTGCTGC TCCCATGGTA CAACGCATAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTG CACAAGGAAG AAGAAGTAGA GTTTCTGTAC
    AACGAGCACA CGGTCCGAGA AAGCCCCAAC ATCACCGACC GGTGGATTCT GTCCTTCATG
    CAGTCGCTCA TTGCGTTCTT TGAGACGGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTG
    CCTCGCCTGG TCAAGTTCGT AGATATTCTG ACCAACTGGT ACGTCCGAAT GAACAGGAGA
    AGATTAAAGG GGGAGAATGG CATGGAAGAC TGTGTTATGG CTCTGGAGAC TCTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACAC CCTTTCTTAC TGAACTGATG
    TACCAGAACC TGAAGCTGCT GATTGACCCT GCATCTATCC GGGACAAGGA CACCCTCAGC
    ATCCACTACC TCATGCTGCC TCATGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GAATGCAGTC CGTGATTGAG CTTGGGAGAG TGATTCGAGA CCGGAAAACT
    ATTCCAATAA AGTATCCCTT GAAGGAAATT GTCGTCATCC ACCAAGACCC AGAAGCTCTT
    GAGGATATCA AGTCCTTGGA GAAGTATATC ATTGAGGAAC TAAATGTTCG AAAAGTTACA
    CTGTCTACTG ATAAAAACAA GTATGGCATT CGACTGAGGG CTGAGCCAGA TCACATGGTC
    CTTGGGAAAC GTCTGAAGGG AGCCTTTAAA GCAGTGATGG TGGCCGTAAA GCAGCTGAGC
    AATGAGGAGC TGGAGCAGTT TCAGAAGAGT GGGAGCATCG TCGTGGAAGG CCATGAATTA
    CACGAAGAAG ATATTCGTCT CATGTACACC TTTGACCAGG CCACAGGAGG CACTGCACAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTTTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGACG AAGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGCAAGAAG
    TGCAACCTGG TTCCGACGGA TGAAATAACA GTTTACTATA ATGCCAAATC TGAAGGAAGA
    TACTTGAGTA ATGTCATCGA AAGCCACACT GACTTCATAT ATGCCACTAT CAAAGCTCCC
    TTGAAGCCCT ATCCAGTTCC TACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTACG AGCTGGAAAT CACGATCACC AGAGGATCCT GTGTGCCTGG GCCCGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACTGAGC AAGGTGGAGT GTTACTCCTG
    GAGAATCCGA AGGGTGATAA TCAGTTGAAC CTTGAGAAGC TGAAGAGCGT CATTACCAGC
    ATATTCGGTG TTAAAAGCAC AGGGCTGTCA GTCTTCAACG GTGGAACAGA ATTACAGAAC
    CAGACTGACT TGCTGAGCCT CAGTGGAAGG ACGCTGTGTG TGACTGCAGG AGCATCCCTG
    GCTCCAGCCA GCAGCCCCAG CACCCTTCTC TGTCAGTACA TCAACCTGCA GCTCGTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTT
    GGACAGAACG GGCTCACCCA CCAAGGGCTT GTGTACGAAG CAGCCAAGGT GTTTGGACTG
    CGCAGCCGGA GGCTAAAGCT GTTCCTGAAT GAGACTCAGA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACGACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006253768.1
    CDS103..3891
    Translation

    Target ORF information:

    RefSeq Version XM_006253706.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus isoleucyl-tRNA synthetase (Iars), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006253706.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCGGA AAACATCAGC TTTCCTGCTG AAGAAGAAAA AATCTTACAG 
    TTCTGGTCCA AATACAACTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGCT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTCGCT ACTGGACTGC CTCACTACGG ACACATACTT
    GCGGGGACAA TTAAAGATAT AGTTACAAGA TACGCTCACC AGAGCGGGTT TCACGTCGAC
    AGAAGATTCG GGTGGGATTG TCATGGCTTA CCTGTGGAGT ACGAGATTGA CAAGACCCTG
    GGGATCAAAG GGCCAGAGGA CGTGGCCAAG ATGGGGATCG CAGAGTACAA CAAGCAGTGC
    CGAGCTATTG TGATGAGGTA CTCCGCCGAG TGGAAGTCTA CTGTTACCAG GCTTGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACC CTGTACCCAC AATTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA AGTCATGCCC
    TTCTCCACCG CGTGTGGAAC TCCACTCTCC AACTTCGAGT CGAACCAGAA TTACAAGGAC
    GTTCAGGACC CTTCAGTATT TGTAACGTTC CCTTTGGAGG AAGATGAAAA CACATCCTTA
    GTTGCTTGGA CAACCACTCC GTGGACCCTA CCCAGTAACC TTGCGCTATG TGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGACGTT TCCAGGGGGA AGTTGTTCAT TTTGATGGAA
    GCCAGATTGT CGGCCCTCTA TAAACAAGAG AGCGACTATG AGATCCTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTTGAC CACTATGTGA AGGATGAAGA AGGCACAGGT
    GTCGTTCACC AGGCTCCTTA CTTTGGCGCT GACGATCATC GAGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCAGC CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTCGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATCAGGATG
    TTGAAGGAGC AGGGCCGACT GCTCGTGGCA AGCACCTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCGG ACACTCCCCT CATTTACAAG TCCGTGCCCA GCTGGTTCGT GCGGGTGGAG
    CACATGGTGG ACCAGCTACT GAAGAACAAT GATCTGTGTT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCACGTG ACTGGGCAAT TTCCCGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGTGAAG ACTTTGAGGA GGTAGTGTGC
    ATCGGGTCAG TGGCAGAACT TGAAGAATTG TCAGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATACCATCC CGCTGCGGGA AAGGACCGTT GCGGCGTGTC
    TCTGAGGTGT TTGACTGTTG GTTCGAGAGT GGAAGTATGC CTTACGCCCA GGTTCATTAC
    CCCTTTGAGA GCAAGCGAGA GTTCGAGGAT GCCTTTCCCG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTAGTGC TAGCCACAGC CCTCTTTGGA
    CAACCACCTT TCAAGAACGT GATTGTGAAC GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAAC GGAAAAAGAA CTACCCAGAT CCGGTTTCCA TCATCAATAA GTACGGTGCC
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTGA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GCGTACGGGA TGTCCTCAAA GACGTGCTGC TCCCATGGTA CAACGCATAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTG CACAAGGAAG AAGAAGTAGA GTTTCTGTAC
    AACGAGCACA CGGTCCGAGA AAGCCCCAAC ATCACCGACC GGTGGATTCT GTCCTTCATG
    CAGTCGCTCA TTGCGTTCTT TGAGACGGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTG
    CCTCGCCTGG TCAAGTTCGT AGATATTCTG ACCAACTGGT ACGTCCGAAT GAACAGGAGA
    AGATTAAAGG GGGAGAATGG CATGGAAGAC TGTGTTATGG CTCTGGAGAC TCTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACAC CCTTTCTTAC TGAACTGATG
    TACCAGAACC TGAAGCTGCT GATTGACCCT GCATCTATCC GGGACAAGGA CACCCTCAGC
    ATCCACTACC TCATGCTGCC TCATGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GAATGCAGTC CGTGATTGAG CTTGGGAGAG TGATTCGAGA CCGGAAAACT
    ATTCCAATAA AGTATCCCTT GAAGGAAATT GTCGTCATCC ACCAAGACCC AGAAGCTCTT
    GAGGATATCA AGTCCTTGGA GAAGTATATC ATTGAGGAAC TAAATGTTCG AAAAGTTACA
    CTGTCTACTG ATAAAAACAA GTATGGCATT CGACTGAGGG CTGAGCCAGA TCACATGGTC
    CTTGGGAAAC GTCTGAAGGG AGCCTTTAAA GCAGTGATGG TGGCCGTAAA GCAGCTGAGC
    AATGAGGAGC TGGAGCAGTT TCAGAAGAGT GGGAGCATCG TCGTGGAAGG CCATGAATTA
    CACGAAGAAG ATATTCGTCT CATGTACACC TTTGACCAGG CCACAGGAGG CACTGCACAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTTTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGACG AAGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGCAAGAAG
    TGCAACCTGG TTCCGACGGA TGAAATAACA GTTTACTATA ATGCCAAATC TGAAGGAAGA
    TACTTGAGTA ATGTCATCGA AAGCCACACT GACTTCATAT ATGCCACTAT CAAAGCTCCC
    TTGAAGCCCT ATCCAGTTCC TACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTACG AGCTGGAAAT CACGATCACC AGAGGATCCT GTGTGCCTGG GCCCGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACTGAGC AAGGTGGAGT GTTACTCCTG
    GAGAATCCGA AGGGTGATAA TCAGTTGAAC CTTGAGAAGC TGAAGAGCGT CATTACCAGC
    ATATTCGGTG TTAAAAGCAC AGGGCTGTCA GTCTTCAACG GTGGAACAGA ATTACAGAAC
    CAGACTGACT TGCTGAGCCT CAGTGGAAGG ACGCTGTGTG TGACTGCAGG AGCATCCCTG
    GCTCCAGCCA GCAGCCCCAG CACCCTTCTC TGTCAGTACA TCAACCTGCA GCTCGTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTT
    GGACAGAACG GGCTCACCCA CCAAGGGCTT GTGTACGAAG CAGCCAAGGT GTTTGGACTG
    CGCAGCCGGA GGCTAAAGCT GTTCCTGAAT GAGACTCAGA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACGACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.