Slc1a4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc1a4
Entrez Gene ID	305540
Full Name	solute carrier family 1 member 4
Synonyms	ASCT1
General protein information	Preferred Names solute carrier family 1 member 4 Names neutral amino acid transporter A neutral amino acid transporter ASCT1 solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q22
Summary	L-serine transporter; involved in uptake of L-serine in primary neurons [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_198763.1	NP_942058.1	neutral amino acid transporter A

Pathways from BioSystems

Homologies

	SLC1A4	XP_001087755.2
Canis lupus familiaris (dog)	SLC1A4	XP_538511.4
Bos taurus (cattle)	SLC1A4	NP_001075046.1
Mus musculus (house mouse)	Slc1a4	NP_061349.3
Rattus norvegicus (Norway rat)	Slc1a4	NP_942058.1
Gallus gallus (chicken)	SLC1A4	XP_001232900.3
Homo sapiens (human)	SLC1A4	NP_003029.2
Pan troglodytes (chimpanzee)	SLC1A4	XP_001166088.1
Danio rerio (zebrafish)	slc1a4	NP_001002513.2
Xenopus tropicalis (tropical clawed frog)	slc1a4	NP_001096391.1

Gene Ontology
Bibliography

Related articles in PubMed

D-Serine Is a Substrate for Neutral Amino Acid Transporters ASCT1/SLC1A4 and ASCT2/SLC1A5, and Is Transported by Both Subtypes in Rat Hippocampal Astrocyte Cultures.
Foster AC, Farnsworth J, Lind GE, Li YX, Yang JY, Dang V, Penjwini M, Viswanath V, Staubli U, Kavanaugh MP
PloS one11(6)e0156551(2016)

Protein kinase C-mediated phosphorylation of RKIP regulates inhibition of Na-alanine cotransport by leukotriene D(4) in intestinal epithelial cells.
Arthur S, Sundaram U
American journal of physiology. Cell physiology307(11)C1010-6(2014 Dec)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Quantitative proteomics identifies a Dab2/integrin module regulating cell migration.
Teckchandani A, Toida N, Goodchild J, Henderson C, Watts J, Wollscheid B, Cooper JA
The Journal of cell biology186(1)99-111(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the selectivity profile of D-serine uptake in cultured rat hippocampal astrocytes is consistent with uptake mediated by both ASCT1 and ASCT2. Together these data suggest that SLC1A4 (ASCT1) may represent an important route of Na-dependent D-serine flux in the brain that has the ability to regulate extracellular D-serine and thereby NMDA receptor activity.
Title: D-Serine Is a Substrate for Neutral Amino Acid Transporters ASCT1/SLC1A4 and ASCT2/SLC1A5, and Is Transported by Both Subtypes in Rat Hippocampal Astrocyte Cultures.

LTD4 inhibits ASCT1 through PKC-mediated phosphorylation of RKIP, leading to the subsequent activation of PKA pathway, possibly through beta2-andrenergic receptor activation.
Title: Protein kinase C-mediated phosphorylation of RKIP regulates inhibition of Na-alanine cotransport by leukotriene D(4) in intestinal epithelial cells.

findings indicate that neurons accumulate a high level of L-serine by using a Na(+)-dependent, high-affinity transport system, operating predominantly through the ASCT1 transporter subtype(ASCT1, SLC1A4).
Title: Characterization of rapid and high-affinity uptake of L-serine in neurons and astrocytes in primary culture.

The following Slc1a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc1a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04994	NM_198763.1 Latest version!	Rattus norvegicus solute carrier family 1 member 4 (Slc1a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc1a4 ( NM_198763.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_942058.1 Rattus norvegicus solute carrier family 1 member 4 (Slc1a4), mRNA.

Price & Availability↑

CloneID	ORa04994
Clone ID Related Accession (Same CDS sequence)	NM_198763.1
Accession Version	NM_198763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	neutral amino acid transporter A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB103401.1. On Dec 17, 2003 this sequence version replaced XM_223649.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB103401.1, FQ210448.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAGA CCAACGGATA CCTCGACGGC GCCCAGGCTC AGCCTGCGGC CCGTCCCCGA 
ACGCCCGAGA CGGGGACGAG CAAAGCGCAG CGCTGTGCTA GCTTTTTTCG GCGCAACGCG 
CTGGTGCTGC TCACCGTGTC GGGGGTGTTG GCGGGCGCCG GCATGGGCGC GGCGCTTCGC 
GGGCTGCAGC TCACCCGCAC GCAAATCACT TACCTGGCCT TCCCGGGCGA GATGCTGCTC 
CGTATGCTGC GCATGATCAT CCTACCGCTG GTGGTTTGCA GTCTGGTGTC GGGCGCCGCC 
TCCCTGGACG CCAGCTCCCT CGGGCGTCTG GGTGGCATCG CTGTTGCTTA CTTCGGCCTC 
ACCACGCTGA GTGCCTCTGC GCTCGCTGTT GCTCTGGCGT TCATCATCAA GCCAGGAGTG 
GGCGCGCAGA CCCTTCATTC CAGCAGCCTG GGGCTGGAGA ACTCAGAACC TCCTCCAGTC 
TCCAAAGAGA CAGTGGACTC TTTCCTGGAT TTACTCAGAA ACCTGTTCCC TTCCAATCTT 
GTGGTTGCCG CATTCTCTAC GTCTGCAACC AGTTACACAG TGGTCGTCCA TAACTCCAGC 
TTGGGGAACG TGACCAAAGA GAAGATCCCC GTCGTCACTG ATGTGAAAGG GATGAACATC 
TTAGGACTGG TCCTTTTTGC CCTGATGTTA GGAGTGGCTC TAAAGAAGCT AGGCCCCGAG 
GGAGACGAGC TCATCCGATT CTTCAATTCC TTCAATGAGG CAACAATGGT GTTGGTGTCG 
TGGATCATGT GGTACGTACC TATCGGCATC ATGTTCCTGA TCGGAAGCAA GATTGTGGAA 
ATGCAGGACC TCATCGTGCT CGTGACTAGC CTCGGGAAAT ACATCTTCGC CTCTATACTG 
GGCCATGTTA TCCATGGAGG AATTGTTCTG CCTCTTGTCT ATTTTGCTTT TACGAGGAAA 
AACCCGTTCA CGTTCCTCCT GGGCCTCCTC ACCCCGTTTG CGACGGCTTT TGCGACCTGT 
TCCAGCTCAG CAACCCTTCC GTCTATGATG AAGTGCATCG AGGAAAACAA TGGCGTGGAC 
AAGAGGATCA GCAGGTTCAT CCTTCCCATC GGGGCCACAG TCAACATGGA TGGGGCCGCC 
ATCTTCCAGT GTGTGGCTGC AGTGTTCATC GCCCAGCTCA ACAACGTGGA CCTGAACGCG 
GGACAGATTT TCACCATTCT GGTGACCGCC ACCGCATCCA GTGTTGGAGC AGCGGGTGTG 
CCGGCTGGAG GGGTCCTCAC CATTGCCATC ATCCTAGAGG CCATTGGACT GCCCACGAAC 
GACCTCTCTC TGATCCTGGC TGTGGACTGG ATTGTGGACA GGACCACCAC TGTGGTGAAC 
GTGGAAGGGG ATGCCCTGGG AGCTGGGATC CTCAACCACC TGAATCAGAG GACAGTGAAG 
AAGGGTGAGC AGGAGCTGCA AGAAGTGAAG GTGGAAGCCA TTCCCAATTC CAAGTCTGAG 
GAGGAGACGT CGCCCCTGGT GACACACCAG AACCGGGCAG GCCCTGTAGC CGTTGCTCCT 
GAACTCGAAT CCAAGGAGTC AGTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942058.1
CDS	14..1603
Translation	MEETNGYLDGAQAQPAARPRTPETGTSKAQRCASFFRRNALVLLTVSGVLAGAGMGAALRGLQLTRTQITYLAFPGEMLLRMLRMIILPLVVCSLVSGAASLDASSLGRLGGIAVAYFGLTTLSASALAVALAFIIKPGVGAQTLHSSSLGLENSEPPPVSKETVDSFLDLLRNLFPSNLVVAAFSTSATSYTVVVHNSSLGNVTKEKIPVVTDVKGMNILGLVLFALMLGVALKKLGPEGDELIRFFNSFNEATMVLVSWIMWYVPIGIMFLIGSKIVEMQDLIVLVTSLGKYIFASILGHVIHGGIVLPLVYFAFTRKNPFTFLLGLLTPFATAFATCSSSATLPSMMKCIEENNGVDKRISRFILPIGATVNMDGAAIFQCVAAVFIAQLNNVDLNAGQIFTILVTATASSVGAAGVPAGGVLTIAIILEAIGLPTNDLSLILAVDWIVDRTTTVVNVEGDALGAGILNHLNQRTVKKGEQELQEVKVEAIPNSKSEEETSPLVTHQNRAGPVAVAPELESKESVL

Target ORF information:

RefSeq Version	NM_198763.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 1 member 4 (Slc1a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198763.1

ORF Insert Sequence:

ATGGAGGAGA CCAACGGATA CCTCGACGGC GCCCAGGCTC AGCCTGCGGC CCGTCCCCGA 
ACGCCCGAGA CGGGGACGAG CAAAGCGCAG CGCTGTGCTA GCTTTTTTCG GCGCAACGCG 
CTGGTGCTGC TCACCGTGTC GGGGGTGTTG GCGGGCGCCG GCATGGGCGC GGCGCTTCGC 
GGGCTGCAGC TCACCCGCAC GCAAATCACT TACCTGGCCT TCCCGGGCGA GATGCTGCTC 
CGTATGCTGC GCATGATCAT CCTACCGCTG GTGGTTTGCA GTCTGGTGTC GGGCGCCGCC 
TCCCTGGACG CCAGCTCCCT CGGGCGTCTG GGTGGCATCG CTGTTGCTTA CTTCGGCCTC 
ACCACGCTGA GTGCCTCTGC GCTCGCTGTT GCTCTGGCGT TCATCATCAA GCCAGGAGTG 
GGCGCGCAGA CCCTTCATTC CAGCAGCCTG GGGCTGGAGA ACTCAGAACC TCCTCCAGTC 
TCCAAAGAGA CAGTGGACTC TTTCCTGGAT TTACTCAGAA ACCTGTTCCC TTCCAATCTT 
GTGGTTGCCG CATTCTCTAC GTCTGCAACC AGTTACACAG TGGTCGTCCA TAACTCCAGC 
TTGGGGAACG TGACCAAAGA GAAGATCCCC GTCGTCACTG ATGTGAAAGG GATGAACATC 
TTAGGACTGG TCCTTTTTGC CCTGATGTTA GGAGTGGCTC TAAAGAAGCT AGGCCCCGAG 
GGAGACGAGC TCATCCGATT CTTCAATTCC TTCAATGAGG CAACAATGGT GTTGGTGTCG 
TGGATCATGT GGTACGTACC TATCGGCATC ATGTTCCTGA TCGGAAGCAA GATTGTGGAA 
ATGCAGGACC TCATCGTGCT CGTGACTAGC CTCGGGAAAT ACATCTTCGC CTCTATACTG 
GGCCATGTTA TCCATGGAGG AATTGTTCTG CCTCTTGTCT ATTTTGCTTT TACGAGGAAA 
AACCCGTTCA CGTTCCTCCT GGGCCTCCTC ACCCCGTTTG CGACGGCTTT TGCGACCTGT 
TCCAGCTCAG CAACCCTTCC GTCTATGATG AAGTGCATCG AGGAAAACAA TGGCGTGGAC 
AAGAGGATCA GCAGGTTCAT CCTTCCCATC GGGGCCACAG TCAACATGGA TGGGGCCGCC 
ATCTTCCAGT GTGTGGCTGC AGTGTTCATC GCCCAGCTCA ACAACGTGGA CCTGAACGCG 
GGACAGATTT TCACCATTCT GGTGACCGCC ACCGCATCCA GTGTTGGAGC AGCGGGTGTG 
CCGGCTGGAG GGGTCCTCAC CATTGCCATC ATCCTAGAGG CCATTGGACT GCCCACGAAC 
GACCTCTCTC TGATCCTGGC TGTGGACTGG ATTGTGGACA GGACCACCAC TGTGGTGAAC 
GTGGAAGGGG ATGCCCTGGG AGCTGGGATC CTCAACCACC TGAATCAGAG GACAGTGAAG 
AAGGGTGAGC AGGAGCTGCA AGAAGTGAAG GTGGAAGCCA TTCCCAATTC CAAGTCTGAG 
GAGGAGACGT CGCCCCTGGT GACACACCAG AACCGGGCAG GCCCTGTAGC CGTTGCTCCT 
GAACTCGAAT CCAAGGAGTC AGTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

D-Serine Is a Substrate for Neutral Amino Acid Transporters ASCT1/SLC1A4 and ASCT2/SLC1A5, and Is Transported by Both Subtypes in Rat Hippocampal Astrocyte Cultures.
PloS one11(6)e0156551(2016)
Foster AC,Farnsworth J,Lind GE,Li YX,Yang JY,Dang V,Penjwini M,Viswanath V,Staubli U,Kavanaugh MP

Protein kinase C-mediated phosphorylation of RKIP regulates inhibition of Na-alanine cotransport by leukotriene D(4) in intestinal epithelial cells.
American journal of physiology. Cell physiology307(11)C1010-6(2014 Dec)
Arthur S,Sundaram U

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Quantitative proteomics identifies a Dab2/integrin module regulating cell migration.
The Journal of cell biology186(1)99-111(2009 Jul)
Teckchandani A,Toida N,Goodchild J,Henderson C,Watts J,Wollscheid B,Cooper JA

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Slc1a4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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