Sf3a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sf3a1
Entrez Gene ID	305479
Full Name	splicing factor 3a, subunit 1
General protein information	Preferred Names splicing factor 3a, subunit 1 Names splicing factor 3A subunit 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107235.1	NP_001100705.1	splicing factor 3A subunit 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sf3a1	NP_001100705.1
Schizosaccharomyces pombe (fission yeast)	sap114	NP_593239.1
Magnaporthe oryzae (rice blast fungus)	MGG_02946	XP_003720761.1
	SF3A1	XP_001109090.1
Canis lupus familiaris (dog)	SF3A1	XP_534733.2
Mus musculus (house mouse)	Sf3a1	NP_080451.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011328	XP_564160.3
Eremothecium gossypii	AGOS_AFR645W	NP_986193.2
Bos taurus (cattle)	SF3A1	NP_001074979.1
Danio rerio (zebrafish)	sf3a1	NP_956388.1
Neurospora crassa	NCU01913	XP_965544.1
Caenorhabditis elegans (roundworm)	prp-21	NP_493746.1
Xenopus tropicalis (tropical clawed frog)	LOC734097	XP_004910689.1
Homo sapiens (human)	SF3A1	NP_005868.1
Pan troglodytes (chimpanzee)	SF3A1	XP_515067.2
Gallus gallus (chicken)	SF3A1	NP_001026643.1
Drosophila melanogaster (fruit fly)	CG16941	NP_650583.1
Kluyveromyces lactis	KLLA0C03322g	XP_452346.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of the human activated spliceosome in three conformational states.
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell research(2018 Jan)

The nuclear cap-binding complex interacts with the U4/U6?U5 tri-snRNP and promotes spliceosome assembly in mammalian cells.
Pabis M, Neufeld N, Steiner MC, Bojic T, Shav-Tal Y, Neugebauer KM
RNA (New York, N.Y.)19(8)1054-63(2013 Aug)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Interaction domains and nuclear targeting signals in subunits of the U2 small nuclear ribonucleoprotein particle-associated splicing factor SF3a.
Huang CJ, Ferfoglia F, Raleff F, Kr?mer A
The Journal of biological chemistry286(15)13106-14(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sf3a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sf3a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03146	NM_001107235.1 Latest version!	Rattus norvegicus splicing factor 3a, subunit 1 (Sf3a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sf3a1 ( NM_001107235.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100705.1 Rattus norvegicus splicing factor 3a, subunit 1 (Sf3a1), mRNA.

Price & Availability↑

CloneID	ORa03146
Clone ID Related Accession (Same CDS sequence)	NM_001107235.1
Accession Version	NM_001107235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	splicing factor 3A subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473963.1. On or before Oct 4, 2007 this sequence version replaced XM_223566.4, XM_001066200.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGCCG GACCCGTGCA GGCGGTACCC CCGCCGCCGC CAGTAGTCAC TGAGCCCAAA 
CAGCCAAATG AAGAAGAAGC ATCATCAAAG GAGGATTCCA CCCCTTCCAA GCCAGTGGTG 
GGGATTATTT ACCCTCCTCC AGAGGTCAGG AATATCGTCG ACAAGACCGC CAGTTTTGTG 
GCCAGAAATG GGCCTGAGTT TGAAGCTAGG ATTCGACAGA ATGAGATCAA CAACCCCAAG 
TTCAATTTCT TAAATCCCAA TGACCCTTAC CATGCCTACT ACCGCCACAA GGTCAGCGAG 
TTCAAGGAGG GGAAGGCTCA GGAACCCTCA GCTGCCATCC CCAAGGTTAT GCAGCAGCAG 
CAGCAAGCCA CACAACAACA GCTGCCCCAG AAGGTCCAAG CCCAGGTGAT CCAAGAGACC 
ATAGTACCGA AGGAGCCCCC TCCTGAGTTC GAGTTCATCG CTGACCCCCC CTCCATCTCA 
GCCTTCGACC TGGATGTAGT GAAGCTGACA GCTCAGTTTG TGGCCAGGAA TGGGCGTCAG 
TTTCTGACCC AGCTGATGCA GAAAGAACAG CGCAACTACC AGTTTGACTT TCTCCGACCC 
CAGCACAGCC TCTTCAATTA CTTCACAAAG CTGGTGGAGC AGTATACCAA GATCTTGATT 
CCACCCAAAG GCTTATTTGC AAAGCTCAAG AAAGAGGCTG AAAATCCCCG AGAAGTTTTG 
GACCAGGTTT GTTACCGAGT AGAATGGGCC AAATTTCAGG AACGTGAGAG GAAGAAGGAG 
GAAGAAGAAA AAGAGAAGGA GCGAGTAGCC TATGCTCAGA TCGACTGGCA TGACTTTGTG 
GTAGTGGAGA CAGTGGATTT TCAACCCAAT GAGCAAGGGA ACTTTCCACC CCCAACAACA 
CCGGAGGAAC TGGGAGCTCG GATCCTCATT CAGGAACGCT ACGAGAAGTT TGGGGAAAGT 
GAGGAGGTGG AGATGGAGGT TGAGTCTGAT GAGGAAGATC AAGAAAAGAC TGAGGAGACT 
CCTTCCCAGC TGGACCAGGA TACTCAAGTA CAGGACATGG ATGAGGGTTC AGACGATGAA 
GAAGAAGGAC AGAAAGTGCC TCCACCCCCA GAGACACCCA TGCCTCCACC TCTGCCCCCA 
ACACCAGACC AAGTCATTGT GCGAAAGGAC TATGATCCCA AAGCTTCCAA GCCCCTTCCT 
CCAGCTCCTG CTCCTGATGA GTATCTTGTG TCCCCCATAA CTGGGGAGAA GATTCCTGCC 
AGCAAAATGC AAGAACACAT GCGCATCGGG CTTCTTGACC CCCGCTGGCT GGAGCAACGG 
GATCGCTCCA TCCGAGAGAA GCAGAGCGAT GATGAGGTGT ACGCACCAGG TCTGGATATT 
GAGAGCAGCC TAAAGCAGTT GGCTGAGCGA CGTACTGACA TCTTTGGGGT AGAAGAGACA 
GCCATTGGTA AGAAGATTGG TGAAGAGGAG ATCCAGAAGC CAGAGGAAAA GGTGACTTGG 
GATGGCCATT CAGGCAGCAT GGCCCGGACC CAGCAGGCTG CCCAGGCCAA CATCACCCTC 
CAGGAGCAGA TTGAGGCCAT CCATAAGGCC AAGGGCCTGG TGCCAGAAGA TGACACCAAA 
GAGAAGATTG GCCCTAGCAA GCCCAATGAA ATCCCTCAGC AGCCACCGCC TCCATCTTCA 
GCCACCAATA TCCCCAGCTC AGCGCCCCCC ATCACGTCAG TTCCCCGGCC ACCTGCGATG 
CCACCTCCAG TCCGCACCAC TGTCGTATCT GCAGTGCCTG TCATGCCTCG GCCCCCCATG 
GCATCTGTGG TCCGGCTGCC CCCAGGCTCA GTGATTGCCC CGATGCCACC CATCATCCAC 
GCACCTAGGA TCAACGTGGT ACCCATGCCT CCTGCAGCAC CTCCCATCAT GGCACCCCGC 
CCACCTCCCA TGATTGTGCC AACAGCCTTT GTGCCTGCTC CCCCTGTGGC TCCAGTGCCA 
GCCCCAGCTC CAATGCCTCC CGTACACCCC CCACCTCCTA TGGAAGATGA GCCTCCATCC 
AAGAAACTAA AGACCGAGGA CAGCCTCATG CCTGAGGAAG AGTTCCTGCG CAGGAACAAG 
GGTCCAGTAT CCATCAAGGT GCAGGTACCC AACATGCAGG ATAAGACAGA ATGGAAGCTG 
AATGGGCAGG GGCTGGTCTT CACTCTCCCG CTCACAGACC AGGTCTCTGT CATCAAAGTG 
AAGATTCATG AAGCCACGGG CATGCCTGCA GGGAAGCAGA AACTACAGTA TGAAGGCATC 
TTCATCAAGG ACTCCAACTC CCTGGCTTAC TACAACATGG CCAGTGGTGC TGTCATCCAC 
TTGGCCCTCA AGGAAAGAGG CGGCCGGAAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100705.1
CDS	133..2508
Translation	MQAGPVQAVPPPPPVVTEPKQPNEEEASSKEDSTPSKPVVGIIYPPPEVRNIVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPNDPYHAYYRHKVSEFKEGKAQEPSAAIPKVMQQQQQATQQQLPQKVQAQVIQETIVPKEPPPEFEFIADPPSISAFDLDVVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYFTKLVEQYTKILIPPKGLFAKLKKEAENPREVLDQVCYRVEWAKFQERERKKEEEEKEKERVAYAQIDWHDFVVVETVDFQPNEQGNFPPPTTPEELGARILIQERYEKFGESEEVEMEVESDEEDQEKTEETPSQLDQDTQVQDMDEGSDDEEEGQKVPPPPETPMPPPLPPTPDQVIVRKDYDPKASKPLPPAPAPDEYLVSPITGEKIPASKMQEHMRIGLLDPRWLEQRDRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPPSSATNIPSSAPPITSVPRPPAMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVPMPPAAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPPSKKLKTEDSLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWKLNGQGLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSLAYYNMASGAVIHLALKERGGRKK

Target ORF information:

RefSeq Version	NM_001107235.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus splicing factor 3a, subunit 1 (Sf3a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107235.1

ORF Insert Sequence:

ATGCAGGCCG GACCCGTGCA GGCGGTACCC CCGCCGCCGC CAGTAGTCAC TGAGCCCAAA 
CAGCCAAATG AAGAAGAAGC ATCATCAAAG GAGGATTCCA CCCCTTCCAA GCCAGTGGTG 
GGGATTATTT ACCCTCCTCC AGAGGTCAGG AATATCGTCG ACAAGACCGC CAGTTTTGTG 
GCCAGAAATG GGCCTGAGTT TGAAGCTAGG ATTCGACAGA ATGAGATCAA CAACCCCAAG 
TTCAATTTCT TAAATCCCAA TGACCCTTAC CATGCCTACT ACCGCCACAA GGTCAGCGAG 
TTCAAGGAGG GGAAGGCTCA GGAACCCTCA GCTGCCATCC CCAAGGTTAT GCAGCAGCAG 
CAGCAAGCCA CACAACAACA GCTGCCCCAG AAGGTCCAAG CCCAGGTGAT CCAAGAGACC 
ATAGTACCGA AGGAGCCCCC TCCTGAGTTC GAGTTCATCG CTGACCCCCC CTCCATCTCA 
GCCTTCGACC TGGATGTAGT GAAGCTGACA GCTCAGTTTG TGGCCAGGAA TGGGCGTCAG 
TTTCTGACCC AGCTGATGCA GAAAGAACAG CGCAACTACC AGTTTGACTT TCTCCGACCC 
CAGCACAGCC TCTTCAATTA CTTCACAAAG CTGGTGGAGC AGTATACCAA GATCTTGATT 
CCACCCAAAG GCTTATTTGC AAAGCTCAAG AAAGAGGCTG AAAATCCCCG AGAAGTTTTG 
GACCAGGTTT GTTACCGAGT AGAATGGGCC AAATTTCAGG AACGTGAGAG GAAGAAGGAG 
GAAGAAGAAA AAGAGAAGGA GCGAGTAGCC TATGCTCAGA TCGACTGGCA TGACTTTGTG 
GTAGTGGAGA CAGTGGATTT TCAACCCAAT GAGCAAGGGA ACTTTCCACC CCCAACAACA 
CCGGAGGAAC TGGGAGCTCG GATCCTCATT CAGGAACGCT ACGAGAAGTT TGGGGAAAGT 
GAGGAGGTGG AGATGGAGGT TGAGTCTGAT GAGGAAGATC AAGAAAAGAC TGAGGAGACT 
CCTTCCCAGC TGGACCAGGA TACTCAAGTA CAGGACATGG ATGAGGGTTC AGACGATGAA 
GAAGAAGGAC AGAAAGTGCC TCCACCCCCA GAGACACCCA TGCCTCCACC TCTGCCCCCA 
ACACCAGACC AAGTCATTGT GCGAAAGGAC TATGATCCCA AAGCTTCCAA GCCCCTTCCT 
CCAGCTCCTG CTCCTGATGA GTATCTTGTG TCCCCCATAA CTGGGGAGAA GATTCCTGCC 
AGCAAAATGC AAGAACACAT GCGCATCGGG CTTCTTGACC CCCGCTGGCT GGAGCAACGG 
GATCGCTCCA TCCGAGAGAA GCAGAGCGAT GATGAGGTGT ACGCACCAGG TCTGGATATT 
GAGAGCAGCC TAAAGCAGTT GGCTGAGCGA CGTACTGACA TCTTTGGGGT AGAAGAGACA 
GCCATTGGTA AGAAGATTGG TGAAGAGGAG ATCCAGAAGC CAGAGGAAAA GGTGACTTGG 
GATGGCCATT CAGGCAGCAT GGCCCGGACC CAGCAGGCTG CCCAGGCCAA CATCACCCTC 
CAGGAGCAGA TTGAGGCCAT CCATAAGGCC AAGGGCCTGG TGCCAGAAGA TGACACCAAA 
GAGAAGATTG GCCCTAGCAA GCCCAATGAA ATCCCTCAGC AGCCACCGCC TCCATCTTCA 
GCCACCAATA TCCCCAGCTC AGCGCCCCCC ATCACGTCAG TTCCCCGGCC ACCTGCGATG 
CCACCTCCAG TCCGCACCAC TGTCGTATCT GCAGTGCCTG TCATGCCTCG GCCCCCCATG 
GCATCTGTGG TCCGGCTGCC CCCAGGCTCA GTGATTGCCC CGATGCCACC CATCATCCAC 
GCACCTAGGA TCAACGTGGT ACCCATGCCT CCTGCAGCAC CTCCCATCAT GGCACCCCGC 
CCACCTCCCA TGATTGTGCC AACAGCCTTT GTGCCTGCTC CCCCTGTGGC TCCAGTGCCA 
GCCCCAGCTC CAATGCCTCC CGTACACCCC CCACCTCCTA TGGAAGATGA GCCTCCATCC 
AAGAAACTAA AGACCGAGGA CAGCCTCATG CCTGAGGAAG AGTTCCTGCG CAGGAACAAG 
GGTCCAGTAT CCATCAAGGT GCAGGTACCC AACATGCAGG ATAAGACAGA ATGGAAGCTG 
AATGGGCAGG GGCTGGTCTT CACTCTCCCG CTCACAGACC AGGTCTCTGT CATCAAAGTG 
AAGATTCATG AAGCCACGGG CATGCCTGCA GGGAAGCAGA AACTACAGTA TGAAGGCATC 
TTCATCAAGG ACTCCAACTC CCTGGCTTAC TACAACATGG CCAGTGGTGC TGTCATCCAC 
TTGGCCCTCA AGGAAAGAGG CGGCCGGAAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of the human activated spliceosome in three conformational states.
Cell research(2018 Jan)
Zhang X,Yan C,Zhan X,Li L,Lei J,Shi Y

The nuclear cap-binding complex interacts with the U4/U6?U5 tri-snRNP and promotes spliceosome assembly in mammalian cells.
RNA (New York, N.Y.)19(8)1054-63(2013 Aug)
Pabis M,Neufeld N,Steiner MC,Bojic T,Shav-Tal Y,Neugebauer KM

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Interaction domains and nuclear targeting signals in subunits of the U2 small nuclear ribonucleoprotein particle-associated splicing factor SF3a.
The Journal of biological chemistry286(15)13106-14(2011 Apr)
Huang CJ,Ferfoglia F,Raleff F,Kr?mer A